FvH4_7g26510

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014805
Physical Location & Seq
Reverse (-)
19998362 .. 20001051
2690 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26510.t1

Sequence Viewer

Length: 1227 bp
ATGTGGGAAGCTCAATCCGATTGGTACTCCCCAACTCGCCTGAACATGAGCCCAAATACCCAGTACGAATTTCCGGGTGATCGGTTCATACCCAATAGGAGTTTGATGGATCTTGATCAAGCCCAGAGCTTATTGACGGACCGAACTATACCGAATCAGAACTCGAATTTCAATGAGGCATACAGAAAGATATTGCAGGATCAACTGACTCTGGATTCAGAAGGGAACCCTTTTAAGATGTTGGTTTTTAGGGGAAGTCCAAAATCTAATAGAAAACCGATTCGTTGTGTTGATGAGATACGCTGGAGTGATGAATTTGATAGTAATGTCAGACCTATTCAGCTTCGGAGTTTACCAAAGGGAGAAAGTAGAATTCTGGATGCACCAAACATGAGAAATGACTTCTACATGAACATCATGGACTGGGGCAAGAACAATGTTCTTGCAGTCGCTTTGGGCCTCGATTTATACCTCTGGAACTCGGACACAGCAAATGTACGTAACCTGTTTCATGTCAATGAGAATGATCACCCTACAAGTTTATCATGGTCTCAAGATGCCAAATATTTGGCAGTTGGATATCAGGGCTCAAAAATCCAACTGTGGGATGCTGAGGCTTCGAAGCTGGTTAGAAGTCTAAAAGGCCATCATAGAAGGGTAGCGACTATTGCATGGAATGGTAACACATTAACATCAGGCAGTCGAGACCGGTCCATTATTAACCATGATGTCAGAGCAAGAAACAATGTGACTTGCCATTTACGTGCACACTCTCAAGAAGTCTGTGGCTTGAAATGGTCGGAAACAGGCAATTTATTGGCAAGCGGTGGCAATGAAAACCTCTTTCTAGTGTACTCGCCTCTGGAGGAGGCACTGAAGATGGGTGCATTAAGTTATGGAATACGCAAAAGGGGACCTGTCTGTGGATTGGAGTGGAACATGCATCACAAAGAGATATTGAGTGGCCACGGTTATAGTCCGAGCGAGCACATCAAGAATCAGCTATGCCTCTGGAGATATCCTTCCATGGAAAAAGTGGGGTACTTGAAACGCCACACCTCCAGAGTCCTTCAGCTTTCTCAGAGCCCTGATGGGTTAACTGTAGTATCAGCTGTTGCAGACGGGACTCTTCGGTTTTGGGATATATTTGGACCCCCTTGTGCTGACAATTTAAGGCTCTCAGCTCTGGATAGTTTTTTGTCTAATAAAACTTCTTTAATAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

409

Amino Acids

46.48

Weight (kDa)

8.79

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 126 - 286 7.4e-60 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 137 - 227 1.1e-07 WDHD1 first WD40 domain
Beta-prop_WDR19_1st PF23389 140 - 260 3e-11 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 145 - 279 4.7e-09 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 145 - 279 3.3e-08 G protein beta WD-40 repeat protein
Beta-prop_Aladin PF25460 148 - 286 1.5e-06 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 149 - 282 9.7e-21 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 149 - 321 7e-13 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 155 - 284 2e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 155 - 281 3.5e-07 WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st PF23383 156 - 240 1.6e-06 IFT140 first beta-propeller
Beta-prop_WDR5 PF25175 157 - 285 2.4e-18 WDR5 beta-propeller domain
ANAPC4_WD40 PF12894 167 - 226 3.4e-07 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_THOC3 PF25174 179 - 382 3.8e-25 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 179 - 291 6.3e-07 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 297 - 380 2e-22 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 825
AclWI GGATC 2 cut(s) 117, 207
AcoI YGGCCR 1 cut(s) 964
AcsI RAATTY 4 cut(s) 68, 166, 314, 372
AcuI CTGAAG 2 cut(s) 896, 1055
AfaI GTAC 5 cut(s) 26, 65, 498, 854, 1043
AfiI CCNNNNNNNGG 2 cut(s) 604, 923
AgeI ACCGGT 1 cut(s) 708
AgsI TTSAA 3 cut(s) 172, 793, 1048
AluBI AGCT 8 cut(s) 11, 129, 343, 625, 1003, 1075, 1112, 1184
AluI AGCT 8 cut(s) 11, 129, 343, 625, 1003, 1075, 1112, 1184
Alw21I GWGCWC 2 cut(s) 769, 990
Alw26I GTCTC 2 cut(s) 555, 699
Alw44I GTGCAC 1 cut(s) 765
AlwI GGATC 2 cut(s) 117, 207
AoxI GGCC 3 cut(s) 457, 643, 964
ApaLI GTGCAC 1 cut(s) 765
ApoI RAATTY 4 cut(s) 68, 166, 314, 372
AsiGI ACCGGT 1 cut(s) 708
AspS9I GGNCC 5 cut(s) 139, 457, 711, 914, 1151
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 2 cut(s) 89, 521
AsuII TTCGAA 1 cut(s) 620
AvaII GGWCC 4 cut(s) 139, 711, 914, 1151
BaeGI GKGCMC 1 cut(s) 769
BaeI ACNNNNGTAYC 2 cut(s) 1089, 1122
BalI TGGCCA 1 cut(s) 966
BanII GRGCYC 3 cut(s) 53, 590, 1088
Bbv12I GWGCWC 2 cut(s) 769, 990
BbvCI CCTCAGC 1 cut(s) 612
BccI CCATC 4 cut(s) 100, 654, 874, 1085
BclI TGATCA 2 cut(s) 115, 526
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 2 cut(s) 555, 699
BfaI CTAG 1 cut(s) 848
BfmI CTRYAG 1 cut(s) 1101
Bme1390I CCNGG 1 cut(s) 75
Bme18I GGWCC 4 cut(s) 139, 711, 914, 1151
BmgT120I GGNCC 5 cut(s) 139, 457, 711, 914, 1151
BmiI GGNNCC 3 cut(s) 227, 915, 1153
BmrFI CCNGG 1 cut(s) 75
BmrI ACTGGG 2 cut(s) 55, 433
BmsI GCATC 4 cut(s) 370, 547, 598, 952
BmuI ACTGGG 2 cut(s) 55, 433
BpmI CTGGAG 4 cut(s) 325, 884, 1033, 1045
Bpu10I CCTNAGC 1 cut(s) 612
Bpu14I TTCGAA 1 cut(s) 620
BpuEI CTTGAG 2 cut(s) 537, 759
BpuMI CCSGG 1 cut(s) 75
BsaAI YACGTR 2 cut(s) 500, 764
BsaBI GATNNNNATC 1 cut(s) 114
BsaI GGTCTC 2 cut(s) 555, 699
BsaJI CCNNGG 2 cut(s) 967, 1026
BsaWI WCCGGW 1 cut(s) 708
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 923
Bse118I RCCGGY 1 cut(s) 708
Bse1I ACTGG 2 cut(s) 61, 428
Bse3DI GCAATG 1 cut(s) 838
Bse8I GATNNNNATC 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 967, 1026
BseGI GGATG 2 cut(s) 385, 613
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 2 cut(s) 604, 923
BseMI GCAATG 1 cut(s) 838
BseMII CTCAG 3 cut(s) 603, 1094, 1194
BseNI ACTGG 2 cut(s) 61, 428
BseRI GAGGAG 1 cut(s) 881
BseSI GKGCMC 1 cut(s) 769
BshFI GGCC 3 cut(s) 459, 645, 966
BshTI ACCGGT 1 cut(s) 708
BsiHKAI GWGCWC 2 cut(s) 769, 990
BsiSI CCGG 2 cut(s) 74, 709
BslFI GGGAC 2 cut(s) 927, 1138
BslI CCNNNNNNNGG 2 cut(s) 604, 923
BsmAI GTCTC 2 cut(s) 555, 699
BsmFI GGGAC 2 cut(s) 927, 1138
BsnI GGCC 3 cut(s) 459, 645, 966
Bso31I GGTCTC 2 cut(s) 555, 699
Bsp119I TTCGAA 1 cut(s) 620
Bsp1286I GDGCHC 5 cut(s) 53, 590, 769, 990, 1088
Bsp143I GATC 5 cut(s) 79, 109, 115, 199, 526
Bsp19I CCATGG 1 cut(s) 1026
BspACI CCGC 1 cut(s) 825
BspANI GGCC 3 cut(s) 459, 645, 966
BspCNI CTCAG 3 cut(s) 604, 1093, 1193
BspLI GGNNCC 3 cut(s) 227, 915, 1153
BspPI GGATC 2 cut(s) 117, 207
BspT104I TTCGAA 1 cut(s) 620
BspTNI GGTCTC 2 cut(s) 555, 699
BsrDI GCAATG 1 cut(s) 838
BsrFI RCCGGY 1 cut(s) 708
BsrI ACTGG 2 cut(s) 61, 428
BssAI RCCGGY 1 cut(s) 708
BssECI CCNNGG 2 cut(s) 967, 1026
BssMI GATC 5 cut(s) 79, 109, 115, 199, 526
BssT1I CCWWGG 1 cut(s) 1026
Bst4CI ACNGT 3 cut(s) 603, 971, 1102
Bst6I CTCTTC 1 cut(s) 1134
BstBAI YACGTR 2 cut(s) 500, 764
BstBI TTCGAA 1 cut(s) 620
BstC8I GCNNGC 2 cut(s) 823, 986
BstDEI CTNAG 3 cut(s) 612, 1080, 1180
BstDSI CCRYGG 2 cut(s) 967, 1026
BstF5I GGATG 2 cut(s) 385, 613
BstKTI GATC 5 cut(s) 82, 112, 118, 202, 529
BstMAI GTCTC 2 cut(s) 555, 699
BstMBI GATC 5 cut(s) 79, 109, 115, 199, 526
BstMWI GCNNNNNNNGC 1 cut(s) 668
BstNSI RCATGY 1 cut(s) 943
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 1101
BstSLI GKGCMC 1 cut(s) 769
BstSNI TACGTA 1 cut(s) 500
BstX2I RGATCY 1 cut(s) 109
BstXI CCANNNNNNTGG 1 cut(s) 568
BstYI RGATCY 1 cut(s) 109
BsuRI GGCC 3 cut(s) 459, 645, 966
BtgI CCRYGG 2 cut(s) 967, 1026
BtsCI GGATG 2 cut(s) 385, 613
BtsIMutI CAGTG 1 cut(s) 872
Cac8I GCNNGC 2 cut(s) 823, 986
Cfr10I RCCGGY 1 cut(s) 708
Cfr13I GGNCC 5 cut(s) 139, 457, 711, 914, 1151
CpoI CGGWCCG 1 cut(s) 139
Csp6I GTAC 5 cut(s) 25, 64, 497, 853, 1042
CspAI ACCGGT 1 cut(s) 708
CspI CGGWCCG 1 cut(s) 139
CviQI GTAC 5 cut(s) 25, 64, 497, 853, 1042
DdeI CTNAG 3 cut(s) 612, 1080, 1180
DpnI GATC 5 cut(s) 81, 111, 117, 201, 528
DpnII GATC 5 cut(s) 79, 109, 115, 199, 526
EaeI YGGCCR 1 cut(s) 964
Eam1104I CTCTTC 1 cut(s) 1134
EarI CTCTTC 1 cut(s) 1134
Eco105I TACGTA 1 cut(s) 500
Eco130I CCWWGG 1 cut(s) 1026
Eco24I GRGCYC 3 cut(s) 53, 590, 1088
Eco31I GGTCTC 2 cut(s) 555, 699
Eco32I GATATC 2 cut(s) 581, 1019
Eco47I GGWCC 4 cut(s) 139, 711, 914, 1151
Eco57I CTGAAG 2 cut(s) 896, 1055
EcoO109I RGGNCCY 1 cut(s) 914
EcoRI GAATTC 1 cut(s) 372
EcoRV GATATC 2 cut(s) 581, 1019
EcoT14I CCWWGG 1 cut(s) 1026
EcoT22I ATGCAT 1 cut(s) 945
EcoT38I GRGCYC 3 cut(s) 53, 590, 1088
ErhI CCWWGG 1 cut(s) 1026
FaqI GGGAC 2 cut(s) 927, 1138
FbaI TGATCA 2 cut(s) 115, 526
FokI GGATG 2 cut(s) 392, 620
FriOI GRGCYC 3 cut(s) 53, 590, 1088
FspBI CTAG 1 cut(s) 848
GsuI CTGGAG 4 cut(s) 325, 884, 1033, 1045
HaeIII GGCC 3 cut(s) 459, 645, 966
HapII CCGG 2 cut(s) 74, 709
HincII GTYRAC 1 cut(s) 1098
HindII GTYRAC 1 cut(s) 1098
HinfI GANTC 7 cut(s) 154, 208, 215, 280, 997, 1065, 1126
HpaI GTTAAC 1 cut(s) 1098
HpaII CCGG 2 cut(s) 74, 709
HphI GGTGA 2 cut(s) 89, 521
Hpy166II GTNNAC 4 cut(s) 353, 767, 853, 1098
Hpy8I GTNNAC 4 cut(s) 353, 767, 853, 1098
HpyAV CCTTC 4 cut(s) 215, 648, 1032, 1079
HpyCH4III ACNGT 3 cut(s) 603, 971, 1102
HpyCH4IV ACGT 2 cut(s) 499, 763
HpyCH4V TGCA 8 cut(s) 196, 383, 446, 671, 767, 887, 943, 1118
HpyF10VI GCNNNNNNNGC 1 cut(s) 668
HpyF3I CTNAG 3 cut(s) 612, 1080, 1180
HpySE526I ACGT 2 cut(s) 499, 763
Ksp22I TGATCA 2 cut(s) 115, 526
KspAI GTTAAC 1 cut(s) 1098
Kzo9I GATC 5 cut(s) 79, 109, 115, 199, 526
LweI GCATC 4 cut(s) 370, 547, 598, 952
MaeI CTAG 1 cut(s) 848
MaeII ACGT 2 cut(s) 499, 763
MaeIII GTNAC 3 cut(s) 500, 680, 748
MalI GATC 5 cut(s) 81, 111, 117, 201, 528
MboI GATC 5 cut(s) 79, 109, 115, 199, 526
MboII GAAGA 2 cut(s) 889, 1121
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 5 cut(s) 53, 590, 769, 990, 1088
MlsI TGGCCA 1 cut(s) 966
MluCI AATT 6 cut(s) 68, 166, 314, 372, 811, 1168
MluNI TGGCCA 1 cut(s) 966
MlyI GAGTC 3 cut(s) 202, 1074, 1120
MmeI TCCRAC 3 cut(s) 556, 622, 780
Mox20I TGGCCA 1 cut(s) 966
Mph1103I ATGCAT 1 cut(s) 945
MscI TGGCCA 1 cut(s) 966
MseI TTAA 7 cut(s) 234, 689, 720, 890, 1097, 1172, 1217
MslI CAYNNNNRTG 2 cut(s) 516, 762
Msp20I TGGCCA 1 cut(s) 966
MspA1I CMGCKG 1 cut(s) 1112
MspI CCGG 2 cut(s) 74, 709
MspR9I CCNGG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 668
NciI CCSGG 1 cut(s) 75
NcoI CCATGG 1 cut(s) 1026
NdeII GATC 5 cut(s) 79, 109, 115, 199, 526
NlaIV GGNNCC 3 cut(s) 227, 915, 1153
NmuCI GTSAC 1 cut(s) 748
NsiI ATGCAT 1 cut(s) 945
NspI RCATGY 1 cut(s) 943
NspV TTCGAA 1 cut(s) 620
PfeI GAWTC 4 cut(s) 154, 215, 280, 997
PinAI ACCGGT 1 cut(s) 708
PleI GAGTC 3 cut(s) 202, 1073, 1120
PpsI GAGTC 3 cut(s) 202, 1073, 1120
Ppu21I YACGTR 2 cut(s) 500, 764
PpuMI RGGWCCY 1 cut(s) 914
Psp5II RGGWCCY 1 cut(s) 914
PspN4I GGNNCC 3 cut(s) 227, 915, 1153
PspPI GGNCC 5 cut(s) 139, 457, 711, 914, 1151
PspPPI RGGWCCY 1 cut(s) 914
PsuI RGATCY 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 1112
RsaI GTAC 5 cut(s) 26, 65, 498, 854, 1043
RsaNI GTAC 5 cut(s) 25, 64, 497, 853, 1042
RseI CAYNNNNRTG 2 cut(s) 516, 762
Rsr2I CGGWCCG 1 cut(s) 139
RsrII CGGWCCG 1 cut(s) 139
SaqAI TTAA 7 cut(s) 234, 689, 720, 890, 1097, 1172, 1217
Sau3AI GATC 5 cut(s) 79, 109, 115, 199, 526
Sau96I GGNCC 5 cut(s) 139, 457, 711, 914, 1151
SchI GAGTC 3 cut(s) 202, 1074, 1120
ScrFI CCNGG 1 cut(s) 75
SduI GDGCHC 5 cut(s) 53, 590, 769, 990, 1088
SfaNI GCATC 4 cut(s) 370, 547, 598, 952
SfcI CTRYAG 1 cut(s) 1101
SfuI TTCGAA 1 cut(s) 620
SinI GGWCC 4 cut(s) 139, 711, 914, 1151
SmiMI CAYNNNNRTG 2 cut(s) 516, 762
SmlI CTYRAG 2 cut(s) 552, 774
SmoI CTYRAG 2 cut(s) 552, 774
SnaBI TACGTA 1 cut(s) 500
Sse9I AATT 6 cut(s) 68, 166, 314, 372, 811, 1168
SsiI CCGC 1 cut(s) 825
SspI AATATT 1 cut(s) 566
SspMI CTAG 1 cut(s) 848
StyD4I CCNGG 1 cut(s) 73
StyI CCWWGG 1 cut(s) 1026
TaaI ACNGT 3 cut(s) 603, 971, 1102
TaiI ACGT 2 cut(s) 502, 766
TaqI TCGA 4 cut(s) 164, 462, 620, 703
TaqII GACCGA 1 cut(s) 156
TasI AATT 6 cut(s) 68, 166, 314, 372, 811, 1168
TatI WGTACW 1 cut(s) 852
TfiI GAWTC 4 cut(s) 154, 215, 280, 997
Tru1I TTAA 7 cut(s) 234, 689, 720, 890, 1097, 1172, 1217
Tru9I TTAA 7 cut(s) 234, 689, 720, 890, 1097, 1172, 1217
TscAI CASTG 1 cut(s) 879
TseFI GTSAC 1 cut(s) 748
Tsp45I GTSAC 1 cut(s) 748
TspDTI ATGAA 5 cut(s) 76, 327, 425, 500, 849
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 1 cut(s) 879
VneI GTGCAC 1 cut(s) 765
VpaK11BI GGWCC 4 cut(s) 139, 711, 914, 1151
XapI RAATTY 4 cut(s) 68, 166, 314, 372
XceI RCATGY 1 cut(s) 943
XcmI CCANNNNNNNNNTGG 1 cut(s) 1033
XspI CTAG 1 cut(s) 848
Zsp2I ATGCAT 1 cut(s) 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.