MD01G1173800.v1.1

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014805
Physical Location & Seq
Reverse (-)
27526649 .. 27529005
2357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1173800.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGCAGGAACTTCAATCGGATTGGTGCTCCCCTACTCGCCTCCAAACCAGCCCCATTGCCCGATTCGATTTTCCGGGAGATCGGTTTATACCCAGTAGGAGTTTGATGGATCTTGATCAAGCCCGCAGTTTATTGACCAACAGAACAACGCCGACCCGCAATTCGAATTTCAATGATGTATACAGACGGAGCTTAGAGGACAAACTGACTTTGGATTCGGATGGGAATCCATTTAGGATGTTGGTTTTTAGGGGAAGTCCGAAATCAAACCGAAAGCCGATCCGTTCTGTTGATCTGATGCGACAGGATGAAGCAAAGGAATTGGACGGTAGTGTTAAAAATGTTCTGCCTCGAAGAATGCCTAAGGGGGAAGCTAGGGTTCTGGATGCAGTAAACATAAAAAATGACTTTTACTCCAGCATCATGGATTGGGGGAAGAACAGTATTCTTGCAGTTGCTCTGGGGAGCGATTTGTTCCTCTGGAACTCGACGAACAGCGAAGTGCACAAGCTGCTGCATGTTGATGATCAGCGTGATTTCCCAACAAGTGTAGCCTGGTCTGAAAATGCTAAAACTTTAGCCGTTGGATATAGGCGATCCAAGCTCCAACTTTGGGATGTTGAGACCTCCAAGCTTGTTAGGAGGTTTGAAAACCATAAGGACAGGATAGCCACCGTTGCGTGGAATGGTCACGTTTTAACTTCCGGCAGCCGCGACACATCCATCATCAACCATGATGTCAGAGCAGGGAGCAATGTGGCTTCCAGATTGCAGGCACACAAAGAAGAAGTGTGCGGTTTGAAATGGTCAGAAGGAGGCAATGTACTGGCAAGTGGAGGCAATGAGAATCTTCTGTACATCTGGAACTCATCGAAAATGAACTCGAGCCGTTTTTTGTTTCGGTTTAGAGAGCATTGTGCTGCAGTCAAGGCCCTCGCATGGTGTCCATATCAGTCTGAGGTGCTTGCCTCTGGTGGAGGCACAGAAGATGGGTGTATTAAGTTATGGAATACAAAAAAGGGAACCTGCATTAAAACCATTGAAACCAATGCTCAGGTCTGTGGACTGGAGTGGAACAGGCATCACAAGGAGATTCTGAGTGCCCATGGCTATAGTCGAGGCGAGCTCATCAAGAATCAGCTGTGCATTTGGAGGTATCCCTCTATGGAGAATGTCGGAAACTTGAATCGCCACAGATCCAGAATCCTCCACGTTTCCCAGAGCCCTGACGGGTTAACTGTGGTATCAGCTGTCGGAGATGAGACACTTCGATTCTGGGAGATCTTCGGACCACCAAGCATTGACAAAATCTCTCCTCTGGATAGTCTCTTATCTCTCAAGACTTCTCCCTTACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

453

Amino Acids

51.14

Weight (kDa)

9.25

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 91 - 229 2.6e-09 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 128 - 426 3.7e-118 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 133 - 297 7.2e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR19_1st PF23389 140 - 242 3.9e-11 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 147 - 288 1.6e-09 WDR36/Utp21 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 148 - 293 9e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_Aladin PF25460 149 - 297 1.1e-07 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 150 - 289 1.4e-22 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 150 - 294 1.5e-13 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 157 - 292 2.8e-07 WDR55
Beta-prop_TEP1_2nd PF25047 167 - 266 4.7e-07 TEP-1 second beta-propeller
ANAPC4_WD40 PF12894 182 - 229 8.4e-07 Anaphase-promoting complex subunit 4 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 182 - 427 5.3e-08 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 182 - 255 3.2e-09 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 182 - 369 6.5e-26 WDR5 beta-propeller domain
WD40_Prp19 PF24814 193 - 325 1.2e-13 Prp19 WD40 domain
WD40_Prp19 PF24814 230 - 428 4.3e-16 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 253 - 368 8.2e-15 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 254 - 347 1.8e-07 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 255 - 349 1.4e-06 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 257 - 367 1.6e-06 EIF3I
WD40_WDHD1_1st PF24817 258 - 322 1e-05 WDHD1 first WD40 domain
Beta-prop_EML PF23409 259 - 370 2.3e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 260 - 368 7.2e-19 THOC3 beta-propeller domain
Beta-prop_EML PF23409 302 - 430 5.2e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 319 - 428 6.4e-07 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1034
AccI GTMKAC 1 cut(s) 180
AccII CGCG 1 cut(s) 714
AciI CCGC 4 cut(s) 124, 157, 712, 795
AclWI GGATC 4 cut(s) 117, 274, 591, 1191
AcsI RAATTY 1 cut(s) 166
AfaI GTAC 2 cut(s) 825, 857
AfiI CCNNNNNNNGG 3 cut(s) 613, 681, 939
AgsI TTSAA 6 cut(s) 14, 172, 650, 802, 1043, 1186
AjnI CCWGG 1 cut(s) 554
AluBI AGCT 8 cut(s) 192, 374, 511, 604, 634, 1126, 1141, 1250
AluI AGCT 8 cut(s) 192, 374, 511, 604, 634, 1126, 1141, 1250
Alw21I GWGCWC 3 cut(s) 29, 507, 1128
Alw26I GTCTC 3 cut(s) 617, 1256, 1331
Alw44I GTGCAC 1 cut(s) 503
AlwI GGATC 4 cut(s) 117, 274, 591, 1191
Ama87I CYCGRG 1 cut(s) 883
AoxI GGCC 1 cut(s) 930
ApaLI GTGCAC 1 cut(s) 503
ApeKI GCWGC 4 cut(s) 511, 514, 708, 920
ApoI RAATTY 1 cut(s) 166
ArsI GACNNNNNNTTYG 4 cut(s) 145, 177, 199, 231
AspS9I GGNCC 2 cut(s) 931, 1289
AsuC2I CCSGG 1 cut(s) 75
AsuII TTCGAA 1 cut(s) 164
AvaI CYCGRG 1 cut(s) 883
AvaII GGWCC 1 cut(s) 1289
AxyI CCTNAGG 1 cut(s) 363
BaeGI GKGCMC 2 cut(s) 507, 1105
BaeI ACNNNNGTAYC 2 cut(s) 1227, 1260
BanII GRGCYC 2 cut(s) 1128, 1226
Bbv12I GWGCWC 3 cut(s) 29, 507, 1128
BbvI GCAGC 4 cut(s) 498, 501, 720, 907
BccI CCATC 4 cut(s) 100, 215, 731, 983
BceAI ACGGC 2 cut(s) 566, 873
BciT130I CCWGG 1 cut(s) 556
BciVI GTATCC 1 cut(s) 1167
BclI TGATCA 2 cut(s) 115, 526
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 3 cut(s) 617, 1256, 1331
BfaI CTAG 1 cut(s) 375
BfmI CTRYAG 2 cut(s) 921, 1111
BfuAI ACCTGC 1 cut(s) 1034
BfuI GTATCC 1 cut(s) 1167
BglII AGATCT 1 cut(s) 1281
BisI GCNGC 5 cut(s) 512, 515, 709, 712, 921
BlsI GCNGC 5 cut(s) 513, 516, 710, 713, 922
Bme1390I CCNGG 2 cut(s) 75, 556
Bme18I GGWCC 1 cut(s) 1289
BmeT110I CYCGRG 1 cut(s) 883
BmgT120I GGNCC 2 cut(s) 931, 1289
BmiI GGNNCC 1 cut(s) 1024
BmrFI CCNGG 2 cut(s) 75, 556
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 4 cut(s) 288, 376, 429, 1090
BmuI ACTGGG 1 cut(s) 87
BplI GAGNNNNNCTC 2 cut(s) 1110, 1142
BpmI CTGGAG 2 cut(s) 400, 1088
Bpu10I CCTNAGC 1 cut(s) 1053
Bpu14I TTCGAA 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 1322
BpuMI CCSGG 1 cut(s) 75
BsaBI GATNNNNATC 2 cut(s) 114, 225
BsaI GGTCTC 1 cut(s) 617
BsaJI CCNNGG 1 cut(s) 1105
BsaXI ACNNNNNCTCC 4 cut(s) 398, 428, 807, 837
Bsc4I CCNNNNNNNGG 3 cut(s) 613, 681, 939
Bse1I ACTGG 3 cut(s) 93, 831, 1071
Bse21I CCTNAGG 1 cut(s) 363
Bse3DI GCAATG 4 cut(s) 54, 760, 826, 847
Bse8I GATNNNNATC 2 cut(s) 114, 225
BseBI CCWGG 1 cut(s) 556
BseDI CCNNGG 1 cut(s) 1105
BseGI GGATG 6 cut(s) 226, 243, 313, 391, 622, 719
BseJI GATNNNNATC 2 cut(s) 114, 225
BseLI CCNNNNNNNGG 3 cut(s) 613, 681, 939
BseMI GCAATG 4 cut(s) 54, 760, 826, 847
BseMII CTCAG 3 cut(s) 948, 1067, 1088
BseNI ACTGG 3 cut(s) 93, 831, 1071
BseRI GAGGAG 1 cut(s) 1305
BseSI GKGCMC 2 cut(s) 507, 1105
BseXI GCAGC 4 cut(s) 498, 501, 720, 907
Bsh1236I CGCG 1 cut(s) 714
BshFI GGCC 1 cut(s) 932
BsiHKAI GWGCWC 3 cut(s) 29, 507, 1128
BsiHKCI CYCGRG 1 cut(s) 883
BsiSI CCGG 2 cut(s) 74, 705
BslI CCNNNNNNNGG 3 cut(s) 613, 681, 939
BsmAI GTCTC 3 cut(s) 617, 1256, 1331
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 1 cut(s) 932
Bso31I GGTCTC 1 cut(s) 617
BsoBI CYCGRG 1 cut(s) 883
Bsp119I TTCGAA 1 cut(s) 164
Bsp1286I GDGCHC 5 cut(s) 29, 507, 1105, 1128, 1226
Bsp1407I TGTACA 1 cut(s) 855
Bsp143I GATC 9 cut(s) 79, 109, 115, 279, 292, 526, 596, 1196, 1281
Bsp19I CCATGG 1 cut(s) 1105
BspACI CCGC 4 cut(s) 124, 157, 712, 795
BspANI GGCC 1 cut(s) 932
BspCNI CTCAG 3 cut(s) 949, 1066, 1089
BspFNI CGCG 1 cut(s) 714
BspLI GGNNCC 1 cut(s) 1024
BspMAI CTGCAG 1 cut(s) 925
BspMI ACCTGC 1 cut(s) 1034
BspPI GGATC 4 cut(s) 117, 274, 591, 1191
BspT104I TTCGAA 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 617
BsrDI GCAATG 4 cut(s) 54, 760, 826, 847
BsrGI TGTACA 1 cut(s) 855
BsrI ACTGG 3 cut(s) 93, 831, 1071
BssECI CCNNGG 1 cut(s) 1105
BssMI GATC 9 cut(s) 79, 109, 115, 279, 292, 526, 596, 1196, 1281
BssNAI GTATAC 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 1105
Bst1107I GTATAC 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 556
Bst4CI ACNGT 4 cut(s) 329, 443, 676, 1240
BstAPI GCANNNNNTGC 1 cut(s) 511
BstAUI TGTACA 1 cut(s) 855
BstBI TTCGAA 1 cut(s) 164
BstC8I GCNNGC 4 cut(s) 124, 774, 966, 1124
BstDEI CTNAG 5 cut(s) 193, 363, 957, 1053, 1097
BstDSI CCRYGG 1 cut(s) 1105
BstF5I GGATG 6 cut(s) 226, 243, 313, 391, 622, 719
BstFNI CGCG 1 cut(s) 714
BstKTI GATC 9 cut(s) 82, 112, 118, 282, 295, 529, 599, 1199, 1284
BstMAI GTCTC 3 cut(s) 617, 1256, 1331
BstMBI GATC 9 cut(s) 79, 109, 115, 279, 292, 526, 596, 1196, 1281
BstMWI GCNNNNNNNGC 4 cut(s) 511, 601, 677, 929
BstNI CCWGG 1 cut(s) 556
BstNSI RCATGY 1 cut(s) 521
BstSCI CCNGG 2 cut(s) 73, 554
BstSFI CTRYAG 2 cut(s) 921, 1111
BstSLI GKGCMC 2 cut(s) 507, 1105
BstUI CGCG 1 cut(s) 714
BstV1I GCAGC 4 cut(s) 498, 501, 720, 907
BstX2I RGATCY 3 cut(s) 109, 1196, 1281
BstXI CCANNNNNNTGG 1 cut(s) 424
BstYI RGATCY 3 cut(s) 109, 1196, 1281
BstZ17I GTATAC 1 cut(s) 181
Bsu36I CCTNAGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 1167
BsuRI GGCC 1 cut(s) 932
BtgI CCRYGG 1 cut(s) 1105
BtsCI GGATG 6 cut(s) 226, 243, 313, 391, 622, 719
BveI ACCTGC 1 cut(s) 1034
Cac8I GCNNGC 4 cut(s) 124, 774, 966, 1124
Cfr13I GGNCC 2 cut(s) 931, 1289
Csp6I GTAC 2 cut(s) 824, 856
CviAII CATG 5 cut(s) 424, 518, 734, 939, 1106
CviQI GTAC 2 cut(s) 824, 856
DdeI CTNAG 5 cut(s) 193, 363, 957, 1053, 1097
DpnI GATC 9 cut(s) 81, 111, 117, 281, 294, 528, 598, 1198, 1283
DpnII GATC 9 cut(s) 79, 109, 115, 279, 292, 526, 596, 1196, 1281
Ecl136II GAGCTC 1 cut(s) 1126
Eco130I CCWWGG 1 cut(s) 1105
Eco24I GRGCYC 2 cut(s) 1128, 1226
Eco31I GGTCTC 1 cut(s) 617
Eco47I GGWCC 1 cut(s) 1289
Eco53kI GAGCTC 1 cut(s) 1126
Eco81I CCTNAGG 1 cut(s) 363
Eco88I CYCGRG 1 cut(s) 883
EcoICRI GAGCTC 1 cut(s) 1126
EcoO109I RGGNCCY 1 cut(s) 931
EcoRII CCWGG 1 cut(s) 554
EcoT14I CCWWGG 1 cut(s) 1105
EcoT38I GRGCYC 2 cut(s) 1128, 1226
ErhI CCWWGG 1 cut(s) 1105
FaeI CATG 5 cut(s) 427, 521, 737, 942, 1109
FatI CATG 5 cut(s) 423, 517, 733, 938, 1105
FauI CCCGC 2 cut(s) 131, 164
FbaI TGATCA 2 cut(s) 115, 526
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 5 cut(s) 512, 515, 709, 712, 921
FokI GGATG 6 cut(s) 233, 250, 320, 398, 629, 706
FriOI GRGCYC 2 cut(s) 1128, 1226
Fsp4HI GCNGC 5 cut(s) 512, 515, 709, 712, 921
FspBI CTAG 1 cut(s) 375
GluI GCNGC 5 cut(s) 512, 515, 709, 712, 921
GsuI CTGGAG 2 cut(s) 400, 1088
HaeIII GGCC 1 cut(s) 932
HapII CCGG 2 cut(s) 74, 705
Hin1II CATG 5 cut(s) 427, 521, 737, 942, 1109
HincII GTYRAC 1 cut(s) 1236
HindII GTYRAC 1 cut(s) 1236
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 9 cut(s) 63, 215, 226, 847, 1093, 1135, 1186, 1203, 1272
HpaI GTTAAC 1 cut(s) 1236
HpaII CCGG 2 cut(s) 74, 705
Hpy166II GTNNAC 5 cut(s) 181, 394, 505, 1064, 1236
Hpy188III TCNNGA 9 cut(s) 113, 383, 481, 765, 862, 1132, 1200, 1319, 1339
Hpy8I GTNNAC 5 cut(s) 181, 394, 505, 1064, 1236
Hpy99I CGWCG 1 cut(s) 493
HpyAV CCTTC 1 cut(s) 806
HpyCH4III ACNGT 4 cut(s) 329, 443, 676, 1240
HpyCH4IV ACGT 2 cut(s) 693, 1212
HpyCH4V TGCA 9 cut(s) 4, 389, 452, 505, 517, 772, 923, 1029, 1146
HpyF10VI GCNNNNNNNGC 4 cut(s) 511, 601, 677, 929
HpyF3I CTNAG 5 cut(s) 193, 363, 957, 1053, 1097
HpySE526I ACGT 2 cut(s) 693, 1212
Hsp92II CATG 5 cut(s) 427, 521, 737, 942, 1109
Ksp22I TGATCA 2 cut(s) 115, 526
KspAI GTTAAC 1 cut(s) 1236
Kzo9I GATC 9 cut(s) 79, 109, 115, 279, 292, 526, 596, 1196, 1281
LmnI GCTCC 5 cut(s) 32, 189, 465, 609, 750
Lsp1109I GCAGC 4 cut(s) 498, 501, 720, 907
LweI GCATC 4 cut(s) 288, 376, 429, 1090
MaeI CTAG 1 cut(s) 375
MaeII ACGT 2 cut(s) 693, 1212
MaeIII GTNAC 1 cut(s) 689
MalI GATC 9 cut(s) 81, 111, 117, 281, 294, 528, 598, 1198, 1283
MboI GATC 9 cut(s) 79, 109, 115, 279, 292, 526, 596, 1196, 1281
MboII GAAGA 6 cut(s) 366, 448, 797, 842, 998, 1276
MflI RGATCY 3 cut(s) 109, 1196, 1281
MhlI GDGCHC 5 cut(s) 29, 507, 1105, 1128, 1226
MluCI AATT 3 cut(s) 160, 166, 320
MmeI TCCRAC 4 cut(s) 565, 631, 1156, 1234
MseI TTAA 5 cut(s) 336, 698, 999, 1032, 1235
MslI CAYNNNNRTG 1 cut(s) 522
MspA1I CMGCKG 2 cut(s) 1141, 1250
MspI CCGG 2 cut(s) 74, 705
MspR9I CCNGG 2 cut(s) 75, 556
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 1 cut(s) 556
MvnI CGCG 1 cut(s) 714
MwoI GCNNNNNNNGC 4 cut(s) 511, 601, 677, 929
NciI CCSGG 1 cut(s) 75
NcoI CCATGG 1 cut(s) 1105
NdeII GATC 9 cut(s) 79, 109, 115, 279, 292, 526, 596, 1196, 1281
NlaIII CATG 5 cut(s) 427, 521, 737, 942, 1109
NlaIV GGNNCC 1 cut(s) 1024
NmuCI GTSAC 1 cut(s) 689
NspI RCATGY 1 cut(s) 521
NspV TTCGAA 1 cut(s) 164
PaeR7I CTCGAG 1 cut(s) 883
PctI GAATGC 1 cut(s) 363
PfeI GAWTC 9 cut(s) 63, 215, 226, 847, 1093, 1135, 1186, 1203, 1272
PfoI TCCNGGA 1 cut(s) 73
PkrI GCNGC 5 cut(s) 513, 516, 710, 713, 922
Psp124BI GAGCTC 1 cut(s) 1128
Psp6I CCWGG 1 cut(s) 554
PspGI CCWGG 1 cut(s) 554
PspN4I GGNNCC 1 cut(s) 1024
PspPI GGNCC 2 cut(s) 931, 1289
PspXI VCTCGAGB 1 cut(s) 883
PstI CTGCAG 1 cut(s) 925
PsuI RGATCY 3 cut(s) 109, 1196, 1281
PvuII CAGCTG 2 cut(s) 1141, 1250
RsaI GTAC 2 cut(s) 825, 857
RsaNI GTAC 2 cut(s) 824, 856
RseI CAYNNNNRTG 1 cut(s) 522
SacI GAGCTC 1 cut(s) 1128
SaqAI TTAA 5 cut(s) 336, 698, 999, 1032, 1235
SatI GCNGC 5 cut(s) 512, 515, 709, 712, 921
Sau3AI GATC 9 cut(s) 79, 109, 115, 279, 292, 526, 596, 1196, 1281
Sau96I GGNCC 2 cut(s) 931, 1289
ScrFI CCNGG 2 cut(s) 75, 556
SduI GDGCHC 5 cut(s) 29, 507, 1105, 1128, 1226
SfaNI GCATC 4 cut(s) 288, 376, 429, 1090
SfcI CTRYAG 2 cut(s) 921, 1111
Sfr274I CTCGAG 1 cut(s) 883
SfuI TTCGAA 1 cut(s) 164
SinI GGWCC 1 cut(s) 1289
SlaI CTCGAG 1 cut(s) 883
SmiMI CAYNNNNRTG 1 cut(s) 522
SmlI CTYRAG 2 cut(s) 883, 1337
SmoI CTYRAG 2 cut(s) 883, 1337
Sse9I AATT 3 cut(s) 160, 166, 320
SsiI CCGC 4 cut(s) 124, 157, 712, 795
SspMI CTAG 1 cut(s) 375
SstI GAGCTC 1 cut(s) 1128
StyD4I CCNGG 2 cut(s) 73, 554
StyI CCWWGG 1 cut(s) 1105
TaaI ACNGT 4 cut(s) 329, 443, 676, 1240
TaiI ACGT 2 cut(s) 696, 1215
TaqI TCGA 8 cut(s) 66, 164, 352, 488, 872, 884, 1117, 1270
TasI AATT 3 cut(s) 160, 166, 320
TatI WGTACW 2 cut(s) 823, 855
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 9 cut(s) 63, 215, 226, 847, 1093, 1135, 1186, 1203, 1272
Tru1I TTAA 5 cut(s) 336, 698, 999, 1032, 1235
Tru9I TTAA 5 cut(s) 336, 698, 999, 1032, 1235
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 4 cut(s) 511, 514, 708, 920
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 2 cut(s) 324, 893
TspGWI ACGGA 2 cut(s) 202, 272
VneI GTGCAC 1 cut(s) 503
VpaK11BI GGWCC 1 cut(s) 1289
XapI RAATTY 1 cut(s) 166
XceI RCATGY 1 cut(s) 521
XhoI CTCGAG 1 cut(s) 883
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.