MD00G1003400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013224
Physical Location & Seq
Forward (+)
544586 .. 548067
3482 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1003400.v1.1.491

Sequence Viewer

Length: 2976 bp
ATGGGTGACAAAAAAGGAGAAACAACCAAGAAAAACCCACAACAACCTCAGCAGCAGCAGCAGCAGCAACAGATTTCACCTTCTCCCAACGACCAACAACCGCCGCCAACGGCTGTTAATATCAGTGGAGCTGCAGCTGCAGCTGGTGTCTCCTTCAATATTCCTCCTCCTTTGTTTGTCCCTACCGGTACTCCAACAACCTCATCGTCATCTCCCTTCGATCAGCATCAATTTGAGGCAGCTGTGAATCCAAAGAGGCCAAGATTTACAAGTACAACCGGACAATGGAAACTTCTTCCTTCTCCATCTTCCCAACAGAAACAACCCCAAATACCAATTCTACCCTCAGAATCAAACCCATCTCGATCTCCAAATCCAAAAAATACTCAATTATCTCAACCCCATGTCTCCACCACCAGAGCAGCCTCCTCCTCCGACACCGCCTCGTCGTGGCCGCCGCACTCTCCTCTACCGTCTCTATCAGCAACATCAGGGCACGACACAAACAAGACAGAGGAAGGAGACCCTCAAAACCCGGTTCACCACCACCAGCAGTTCAGAAAAGGAAAGTATGTGAGTCCGGTTTGGAAACCAAACGAGATGCTATGGCTGGCTAGGGGATGGAGGATTCAGTACCAAGGTGGGGCAGCCCGTGGATCTTCGGATCATGGGTCGGGCTCATCTTCAAGAGCAGAGCAGTTACAAACAGAGACAGGCGGCGCCACCACCACTACTCACACCAGAAACAAAACGAGGGCTGACAAGGACAGGGAAGTCGCCGACTTCCTTCAGAAGCATGGCGTCAACAGGGACGCGAAAACGGCGGGAACCAAGTGGGATAACATGCTGGGAGAGTTTAGAAAGGTTTATGAATGGGAGAGAGGTTCCGAGAGAGAACAAGTTGGAAAGAGTTACTTTAGGCTTTCACCGTACGAGAGAAAGCTTCATAGGTTGCCTGCTTCTTTTGATGAGGAGGTTTTCGAGGAGCTCTCGCAGTTCATGGGGTCGAGGATGAGGACTACTCAGAGCAGATTAGGGTCGGTTGGGGACGACAGTCGATCGGCTCTTGTTATGACAAGAAGTCTTCCCCAACCGCCTTCGTTCAAAGAAGACGACTTCCCTCTCTCAGGTAGGGGAAAGCAACTAATGATCATGAGCGGTGGAGGGGAACCGTTTTACCACGGAGGAAGAGGGACCTTGCTAGGGTTTCCTCATCATGATCAGTCTTCCCTAGACTTTGCAACTGGCCTCTCTTCATCTTCTTCCAAGGAGCTCCGTCGAATCGGAAAGATTCGAATGACGTGGGAAGAATCGGTGAGTCTGTGGGCGGAAGAAGGGGAGCACCACAGAGGGAGACTGAGGCTACAAGGCTCGAGCTTCTTGAATGCCGACGAGCTCACTTTCTTTGATGATTCCATGGTTGCTTGCACCATGGAAGCCTTCGAAGATGGCCCCTTTCGAGGCTTCTCCGTTGACCGATTTGTGTCCGGACAACAAGTCAAAGTCTTTGGTAGAAGAAGACCTTCATCCGCTACTTCTGCAATGCCTCCATTTGAATTTCAAGACCCAACGGAGTACTACGTGGGGTGTCTTCGAGTCCCACCGCCATCACTTCCTACATTATCCGAGCTTCAATGGTACGTACAAGAGCCACCGCCGGAGGAACTCCGTTTCCCACTCCGAAAAGACTTGTACCGCGACTTACCACAAGGGAAAGAGATCTTCTTCACCACCTCAACCGAATTGCTAGACTGTAGAGCCATCACATACGATATTCTAAGCCCCATAATCCGAACCAATCCAAGTCTTAGTGCTGCCACTGCCACTGCCACAAGTAGAGACTCCTTCATTCCCATTTGGGACGACTGCATCAACCGAATCGTCTCCAAATTTTGTTCAGTGGAAATGGTCTTCATCCGCAAGCCTAACGCTTCATCCCCAACTGAACCATTACAGGATCAATGGCCGAACGTGACAGGCTTCGTTCGAAATTTCTGTCTATGGAGAGGGGAAGAAGCCGATAATTTAAGAGATGACCGGGACATTAATCCGTCAAGCTCAATAGTTGAGAAACTTTTGTGGACTTACATGGATCTTCCTTACATATTAGGGTACTACGCAATCGGTCATGTTGTAACCTTCTGTGCATTGAGTCGGGTTCAAGATCGAATAATTCGCACTGATTTAGGAACCGTTGATCTGTCATCGACCTCTGAGAGACTGAAAGCCCTAGTCCCATGTTTCAGGATTGCAAGCCTATTGCCATTGCTAGCGGATCGATGCTTCAACATTAGTAGTAATTGCAATGGTGGTTCTTACAAAATGCCACCTTATAGCGATTTCGAGAGGATCGACATGGGGAGCGGAAACGCAATTGAAATGACGCCAACCACAGTGACTCGATTTTACAGTAACATTCGGAAGGCGACCGCCGTAAAGGAAATCTACGATTTTCTCGACCAGAGAATTCCCCATGCAGAGTACATCCACAGATCGTTGGAGAAAGGTTGGGCTTTGGTGTTTAAGCCTAGAGGGTGCAAGTTCAAGCCTACAAATTGCGACCAGCTCGTTGAGGCTCTCATGTACGTGACAAAAGCGCTGGTGGCATTGCACGACTTGTCGTTTATGCATAGGGATATATGTTGGGAGAAAGTGATGAGGAATGAGAGGGACAACGAGTGGTTTGTTTGCGGGTTCGAGGAGGCTGTCGGGGCTCCGCAGATATACCCGCACAGTGTGGGGATGGCTGGAGATGCGGTGGCGCGTGGGAGGCACGCGCCGGAGATGGAGAGAGGGTTGCATGGGGTGAAGGTGGACGTGTGGGGAGTAGGGAATCTGGTGAGGAGTTGCGGGTTGACAGGTGTGCCGAAGTTGCTGAGGGAGCTCCAAAACCGGTGTTTGGATCAGAACCCGGAGAAGAGGCCAACTGCAGCCGACTGTTACCACCACCTGCTGCAGCTGCAGTCGTCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

992

Amino Acids

111.19

Weight (kDa)

7.83

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51800
fragaria_vesca FvH4_7g28640 FvH4_7g28640
malus_domestica MD00G1003400.v1.1 MD01G1192000.v1.1
prunus_persica Prupe.2G286400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0376121
rosa_laevigata RLG00000026631
rosa_multiflora Rmu_ssc0000116.1_g000035
rosa_roxburghii Rroxscaffold_4G00282390
rosa_rugosa Rorug01G0393900 Rorug01G0394000
rosa_samantha Rh1AG405300 Rh1BG368900 Rh1BG369000 Rh1CG381900 Rh1DG398800
rosa_wichuraiana Rw1G035970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2958
AasI GACNNNNNNGTC 1 cut(s) 773
Acc36I ACCTGC 1 cut(s) 2958
AccB1I GGYRCC 1 cut(s) 719
AccBSI CCGCTC 2 cut(s) 1158, 2364
AccII CGCG 4 cut(s) 815, 1698, 2764, 2777
AccIII TCCGGA 1 cut(s) 1487
AclWI GGATC 7 cut(s) 664, 672, 1965, 2100, 2283, 2357, 2910
AcoI YGGCCR 2 cut(s) 452, 1964
AcsI RAATTY 4 cut(s) 1556, 1889, 1990, 2466
AcuI CTGAAG 1 cut(s) 773
AcyI GRCGYC 3 cut(s) 720, 801, 2384
AdeI CACNNNGTG 1 cut(s) 2737
AfeI AGCGCT 1 cut(s) 2598
AfiI CCNNNNNNNGG 7 cut(s) 285, 450, 643, 1127, 1203, 1460, 2899
AflIII ACRYGT 1 cut(s) 2817
AgeI ACCGGT 2 cut(s) 185, 2892
AhdI GACNNNNNGTC 2 cut(s) 1080, 1496
AjiI CACGTC 2 cut(s) 1302, 2818
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AloI GAACNNNNNNTCC 2 cut(s) 1656, 1688
Alw21I GWGCWC 5 cut(s) 990, 1275, 1344, 1398, 2886
Alw26I GTCTC 9 cut(s) 154, 412, 480, 516, 704, 1348, 1833, 1888, 2212
AlwI GGATC 7 cut(s) 664, 672, 1965, 2100, 2283, 2357, 2910
Ama87I CYCGRG 1 cut(s) 1372
Aor13HI TCCGGA 1 cut(s) 1487
Aor51HI AGCGCT 1 cut(s) 2598
AoxI GGCC 6 cut(s) 257, 452, 1246, 1450, 1964, 2921
ApoI RAATTY 4 cut(s) 1556, 1889, 1990, 2466
ArsI GACNNNNNNTTYG 2 cut(s) 1981, 2013
AseI ATTAAT 1 cut(s) 2046
AsiGI ACCGGT 2 cut(s) 185, 2892
Asp700I GAANNNNTTC 1 cut(s) 1522
AspLEI GCGC 4 cut(s) 722, 2599, 2764, 2779
AspS9I GGNCC 2 cut(s) 1194, 1451
AsuC2I CCSGG 3 cut(s) 536, 2039, 2912
AsuHPI GGTGA 8 cut(s) 17, 69, 533, 918, 1327, 1720, 2818, 2851
AsuII TTCGAA 3 cut(s) 1294, 1443, 1987
AsuNHI GCTAGC 1 cut(s) 2269
AvaI CYCGRG 1 cut(s) 1372
AvaII GGWCC 1 cut(s) 1194
BaeGI GKGCMC 1 cut(s) 498
BanI GGYRCC 1 cut(s) 719
BanII GRGCYC 6 cut(s) 680, 990, 1275, 1398, 2716, 2886
BbsI GAAGAC 6 cut(s) 1076, 1116, 1218, 1525, 1583, 1903
Bbv12I GWGCWC 5 cut(s) 990, 1275, 1344, 1398, 2886
BbvCI CCTCAGC 2 cut(s) 48, 2876
BccI CCATC 8 cut(s) 313, 367, 615, 1442, 1615, 1769, 2737, 2779
BceAI ACGGC 3 cut(s) 126, 837, 2417
BclI TGATCA 2 cut(s) 1149, 1219
BcnI CCSGG 3 cut(s) 536, 2039, 2912
BcoDI GTCTC 9 cut(s) 154, 412, 480, 516, 704, 1348, 1833, 1888, 2212
BfaI CTAG 7 cut(s) 615, 1202, 1232, 1748, 2231, 2270, 2529
BfmI CTRYAG 7 cut(s) 132, 138, 1753, 2928, 2954, 2960, 2972
BfoI RGCGCY 2 cut(s) 723, 2600
BfuAI ACCTGC 1 cut(s) 2958
BglII AGATCT 1 cut(s) 1719
BmcAI AGTACT 1 cut(s) 1577
Bme1390I CCNGG 3 cut(s) 536, 2039, 2912
Bme18I GGWCC 1 cut(s) 1194
BmeRI GACNNNNNGTC 2 cut(s) 1080, 1496
BmeT110I CYCGRG 1 cut(s) 1372
BmgBI CACGTC 2 cut(s) 1302, 2818
BmgT120I GGNCC 2 cut(s) 1194, 1451
BmiI GGNNCC 8 cut(s) 721, 829, 886, 1170, 1195, 1453, 2191, 2715
BmrFI CCNGG 3 cut(s) 536, 2039, 2912
BmsI GCATC 5 cut(s) 235, 591, 1878, 2270, 2743
BmtI GCTAGC 1 cut(s) 2273
BoxI GACNNNNGTC 1 cut(s) 1053
BpiI GAAGAC 6 cut(s) 1076, 1116, 1218, 1525, 1583, 1903
BplI GAGNNNNNCTC 4 cut(s) 974, 1006, 1006, 1038
BpmI CTGGAG 1 cut(s) 2769
Bpu10I CCTNAGC 2 cut(s) 48, 2876
Bpu14I TTCGAA 3 cut(s) 1294, 1443, 1987
BpuMI CCSGG 3 cut(s) 536, 2039, 2912
Bsa29I ATCGAT 1 cut(s) 2278
BsaAI YACGTR 3 cut(s) 1582, 1642, 2587
BsaBI GATNNNNATC 1 cut(s) 225
BsaHI GRCGYC 3 cut(s) 720, 801, 2384
BsaI GGTCTC 1 cut(s) 516
BsaJI CCNNGG 6 cut(s) 637, 652, 1180, 1266, 1416, 1431
BsaWI WCCGGW 5 cut(s) 185, 278, 580, 1487, 2892
BsaXI ACNNNNNCTCC 6 cut(s) 175, 205, 2742, 2772, 2780, 2810
Bsc4I CCNNNNNNNGG 7 cut(s) 285, 450, 643, 1127, 1203, 1460, 2899
Bse118I RCCGGY 2 cut(s) 185, 2892
Bse1I ACTGG 1 cut(s) 1249
Bse3DI GCAATG 4 cut(s) 1548, 2264, 2311, 2606
Bse8I GATNNNNATC 1 cut(s) 225
BseAI TCCGGA 1 cut(s) 1487
BseCI ATCGAT 1 cut(s) 2278
BseDI CCNNGG 6 cut(s) 637, 652, 1180, 1266, 1416, 1431
BseGI GGATG 7 cut(s) 626, 1017, 1526, 1914, 1934, 2484, 2748
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 7 cut(s) 285, 450, 643, 1127, 1203, 1460, 2899
BseMI GCAATG 4 cut(s) 1548, 2264, 2311, 2606
BseMII CTCAG 7 cut(s) 62, 360, 1037, 1140, 1349, 2205, 2867
BseNI ACTGG 1 cut(s) 1249
BseRI GAGGAG 8 cut(s) 156, 418, 421, 456, 986, 998, 2714, 2857
BseSI GKGCMC 1 cut(s) 498
BseYI CCCAGC 1 cut(s) 847
Bsh1236I CGCG 4 cut(s) 815, 1698, 2764, 2777
Bsh1285I CGRYCG 2 cut(s) 1061, 2430
BshFI GGCC 6 cut(s) 259, 454, 1248, 1452, 1966, 2923
BshNI GGYRCC 1 cut(s) 719
BshTI ACCGGT 2 cut(s) 185, 2892
BshVI ATCGAT 1 cut(s) 2278
BsiEI CGRYCG 2 cut(s) 1061, 2430
BsiHKAI GWGCWC 5 cut(s) 990, 1275, 1344, 1398, 2886
BsiHKCI CYCGRG 1 cut(s) 1372
BsiWI CGTACG 1 cut(s) 930
BslFI GGGAC 9 cut(s) 164, 824, 1061, 1207, 1583, 1874, 2054, 2219, 2684
BslI CCNNNNNNNGG 7 cut(s) 285, 450, 643, 1127, 1203, 1460, 2899
BsmAI GTCTC 9 cut(s) 154, 412, 480, 516, 704, 1348, 1833, 1888, 2212
BsmBI CGTCTC 2 cut(s) 480, 1888
BsmFI GGGAC 9 cut(s) 164, 824, 1061, 1207, 1583, 1874, 2054, 2219, 2684
BsmI GAATGC 1 cut(s) 1390
BsnI GGCC 6 cut(s) 259, 454, 1248, 1452, 1966, 2923
Bso31I GGTCTC 1 cut(s) 516
BsoBI CYCGRG 1 cut(s) 1372
Bsp119I TTCGAA 3 cut(s) 1294, 1443, 1987
Bsp1286I GDGCHC 8 cut(s) 498, 680, 990, 1275, 1344, 1398, 2716, 2886
Bsp13I TCCGGA 1 cut(s) 1487
Bsp19I CCATGG 2 cut(s) 1416, 1431
BspANI GGCC 6 cut(s) 259, 454, 1248, 1452, 1966, 2923
BspCNI CTCAG 7 cut(s) 61, 359, 1036, 1139, 1350, 2206, 2868
BspDI ATCGAT 1 cut(s) 2278
BspEI TCCGGA 1 cut(s) 1487
BspFNI CGCG 4 cut(s) 815, 1698, 2764, 2777
BspHI TCATGA 2 cut(s) 1152, 1216
BspLI GGNNCC 8 cut(s) 721, 829, 886, 1170, 1195, 1453, 2191, 2715
BspMAI CTGCAG 5 cut(s) 136, 142, 2932, 2958, 2964
BspMI ACCTGC 1 cut(s) 2958
BspOI GCTAGC 1 cut(s) 2273
BspPI GGATC 7 cut(s) 664, 672, 1965, 2100, 2283, 2357, 2910
BspT104I TTCGAA 3 cut(s) 1294, 1443, 1987
BspT107I GGYRCC 1 cut(s) 719
BspTNI GGTCTC 1 cut(s) 516
BsrBI CCGCTC 2 cut(s) 1158, 2364
BsrDI GCAATG 4 cut(s) 1548, 2264, 2311, 2606
BsrFI RCCGGY 2 cut(s) 185, 2892
BsrI ACTGG 1 cut(s) 1249
BssAI RCCGGY 2 cut(s) 185, 2892
BssECI CCNNGG 6 cut(s) 637, 652, 1180, 1266, 1416, 1431
BssNI GRCGYC 3 cut(s) 720, 801, 2384
BssT1I CCWWGG 4 cut(s) 637, 1266, 1416, 1431
Bst6I CTCTTC 3 cut(s) 1183, 1258, 2912
BstACI GRCGYC 3 cut(s) 720, 801, 2384
BstBAI YACGTR 3 cut(s) 1582, 1642, 2587
BstBI TTCGAA 3 cut(s) 1294, 1443, 1987
BstC8I GCNNGC 7 cut(s) 612, 957, 1426, 1922, 2254, 2271, 2775
BstDEI CTNAG 9 cut(s) 48, 346, 1023, 1126, 1358, 1778, 1808, 2214, 2876
BstDSI CCRYGG 4 cut(s) 652, 1180, 1416, 1431
BstENI CCTNNNNNAGG 1 cut(s) 1125
BstF5I GGATG 7 cut(s) 626, 1017, 1526, 1914, 1934, 2484, 2748
BstFNI CGCG 4 cut(s) 815, 1698, 2764, 2777
BstH2I RGCGCY 2 cut(s) 723, 2600
BstHHI GCGC 4 cut(s) 722, 2599, 2764, 2779
BstMAI GTCTC 9 cut(s) 154, 412, 480, 516, 704, 1348, 1833, 1888, 2212
BstMCI CGRYCG 2 cut(s) 1061, 2430
BstNSI RCATGY 1 cut(s) 847
BstPAI GACNNNNGTC 1 cut(s) 1053
BstSCI CCNGG 3 cut(s) 534, 2037, 2910
BstSFI CTRYAG 7 cut(s) 132, 138, 1753, 2928, 2954, 2960, 2972
BstSLI GKGCMC 1 cut(s) 498
BstSNI TACGTA 1 cut(s) 1642
BstUI CGCG 4 cut(s) 815, 1698, 2764, 2777
BstV2I GAAGAC 6 cut(s) 1076, 1116, 1218, 1525, 1583, 1903
BstX2I RGATCY 3 cut(s) 656, 1719, 2092
BstYI RGATCY 3 cut(s) 656, 1719, 2092
Bsu15I ATCGAT 1 cut(s) 2278
BsuRI GGCC 6 cut(s) 259, 454, 1248, 1452, 1966, 2923
BsuTUI ATCGAT 1 cut(s) 2278
BtgI CCRYGG 4 cut(s) 652, 1180, 1416, 1431
BtrI CACGTC 2 cut(s) 1302, 2818
BtsCI GGATG 7 cut(s) 626, 1017, 1526, 1914, 1934, 2484, 2748
BtsI GCAGTG 2 cut(s) 1818, 1824
BtsIMutI CAGTG 7 cut(s) 130, 1818, 1824, 1905, 2178, 2400, 2740
BveI ACCTGC 1 cut(s) 2958
Cac8I GCNNGC 7 cut(s) 612, 957, 1426, 1922, 2254, 2271, 2775
CciI TCATGA 2 cut(s) 1152, 1216
CfoI GCGC 4 cut(s) 722, 2599, 2764, 2779
Cfr10I RCCGGY 2 cut(s) 185, 2892
Cfr13I GGNCC 2 cut(s) 1194, 1451
ClaI ATCGAT 1 cut(s) 2278
CseI GACGC 3 cut(s) 790, 821, 2392
CspAI ACCGGT 2 cut(s) 185, 2892
DdeI CTNAG 9 cut(s) 48, 346, 1023, 1126, 1358, 1778, 1808, 2214, 2876
DinI GGCGCC 1 cut(s) 721
DraIII CACNNNGTG 1 cut(s) 2737
DrdI GACNNNNNNGTC 1 cut(s) 773
DriI GACNNNNNGTC 2 cut(s) 1080, 1496
DseDI GACNNNNNNGTC 1 cut(s) 773
EaeI YGGCCR 2 cut(s) 452, 1964
Eam1104I CTCTTC 3 cut(s) 1183, 1258, 2912
Eam1105I GACNNNNNGTC 2 cut(s) 1080, 1496
EarI CTCTTC 3 cut(s) 1183, 1258, 2912
EciI GGCGGA 1 cut(s) 1343
Ecl136II GAGCTC 4 cut(s) 988, 1273, 1396, 2884
Eco105I TACGTA 1 cut(s) 1642
Eco130I CCWWGG 4 cut(s) 637, 1266, 1416, 1431
Eco24I GRGCYC 6 cut(s) 680, 990, 1275, 1398, 2716, 2886
Eco31I GGTCTC 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 1194
Eco47III AGCGCT 1 cut(s) 2598
Eco53kI GAGCTC 4 cut(s) 988, 1273, 1396, 2884
Eco57I CTGAAG 1 cut(s) 773
Eco88I CYCGRG 1 cut(s) 1372
EcoICRI GAGCTC 4 cut(s) 988, 1273, 1396, 2884
EcoNI CCTNNNNNAGG 1 cut(s) 1125
EcoO109I RGGNCCY 1 cut(s) 1194
EcoRI GAATTC 1 cut(s) 2466
EcoT14I CCWWGG 4 cut(s) 637, 1266, 1416, 1431
EcoT22I ATGCAT 1 cut(s) 2631
EcoT38I GRGCYC 6 cut(s) 680, 990, 1275, 1398, 2716, 2886
EgeI GGCGCC 1 cut(s) 721
EheI GGCGCC 1 cut(s) 721
ErhI CCWWGG 4 cut(s) 637, 1266, 1416, 1431
Esp3I CGTCTC 2 cut(s) 480, 1888
FalI AAGNNNNNCTT 4 cut(s) 899, 931, 1507, 1539
FaqI GGGAC 9 cut(s) 164, 824, 1061, 1207, 1583, 1874, 2054, 2219, 2684
FauI CCCGC 4 cut(s) 817, 2684, 2736, 2843
FbaI TGATCA 2 cut(s) 1149, 1219
FokI GGATG 7 cut(s) 633, 1024, 1513, 1901, 1921, 2471, 2755
FriOI GRGCYC 6 cut(s) 680, 990, 1275, 1398, 2716, 2886
FspBI CTAG 7 cut(s) 615, 1202, 1232, 1748, 2231, 2270, 2529
GlaI GCGC 4 cut(s) 721, 2598, 2763, 2778
GsaI CCCAGC 1 cut(s) 851
GsuI CTGGAG 1 cut(s) 2769
HaeII RGCGCY 2 cut(s) 723, 2600
HaeIII GGCC 6 cut(s) 259, 454, 1248, 1452, 1966, 2923
HgaI GACGC 3 cut(s) 790, 821, 2392
HhaI GCGC 4 cut(s) 722, 2599, 2764, 2779
Hin1I GRCGYC 3 cut(s) 720, 801, 2384
Hin6I GCGC 4 cut(s) 720, 2597, 2762, 2777
HinP1I GCGC 4 cut(s) 720, 2597, 2762, 2777
HincII GTYRAC 3 cut(s) 805, 1474, 2856
HindII GTYRAC 3 cut(s) 805, 1474, 2856
HindIII AAGCTT 1 cut(s) 941
HphI GGTGA 8 cut(s) 17, 69, 533, 918, 1327, 1720, 2818, 2851
Hpy166II GTNNAC 6 cut(s) 541, 805, 1474, 2082, 2815, 2856
Hpy8I GTNNAC 6 cut(s) 541, 805, 1474, 2082, 2815, 2856
Hpy99I CGWCG 3 cut(s) 451, 1281, 1394
HpyCH4IV ACGT 6 cut(s) 1301, 1581, 1641, 1971, 2586, 2817
HpyF3I CTNAG 9 cut(s) 48, 346, 1023, 1126, 1358, 1778, 1808, 2214, 2876
HpySE526I ACGT 6 cut(s) 1301, 1581, 1641, 1971, 2586, 2817
Hsp92I GRCGYC 3 cut(s) 720, 801, 2384
HspAI GCGC 4 cut(s) 720, 2597, 2762, 2777
KasI GGCGCC 1 cut(s) 719
Kpn2I TCCGGA 1 cut(s) 1487
Ksp22I TGATCA 2 cut(s) 1149, 1219
LmnI GCTCC 9 cut(s) 128, 985, 1270, 1278, 1339, 2361, 2719, 2881, 2889
LweI GCATC 5 cut(s) 235, 591, 1878, 2270, 2743
MaeI CTAG 7 cut(s) 615, 1202, 1232, 1748, 2231, 2270, 2529
MaeII ACGT 6 cut(s) 1301, 1581, 1641, 1971, 2586, 2817
MaeIII GTNAC 9 cut(s) 5, 699, 911, 1972, 2134, 2395, 2411, 2587, 2939
MbiI CCGCTC 2 cut(s) 1158, 2364
MfeI CAATTG 1 cut(s) 2373
MflI RGATCY 3 cut(s) 656, 1719, 2092
MhlI GDGCHC 8 cut(s) 498, 680, 990, 1275, 1344, 1398, 2716, 2886
Mly113I GGCGCC 1 cut(s) 720
MlyI GAGTC 6 cut(s) 586, 1327, 1605, 1835, 2161, 2392
MmeI TCCRAC 4 cut(s) 218, 459, 883, 2478
Mph1103I ATGCAT 1 cut(s) 2631
MroI TCCGGA 1 cut(s) 1487
MroXI GAANNNNTTC 1 cut(s) 1522
MseI TTAA 4 cut(s) 117, 2027, 2046, 2523
MslI CAYNNNNRTG 1 cut(s) 2585
MspA1I CMGCKG 4 cut(s) 137, 143, 242, 2959
MspR9I CCNGG 3 cut(s) 536, 2039, 2912
MunI CAATTG 1 cut(s) 2373
Mva1269I GAATGC 1 cut(s) 1390
MvnI CGCG 4 cut(s) 815, 1698, 2764, 2777
NarI GGCGCC 1 cut(s) 720
NciI CCSGG 3 cut(s) 536, 2039, 2912
NcoI CCATGG 2 cut(s) 1416, 1431
NheI GCTAGC 1 cut(s) 2269
NlaIV GGNNCC 8 cut(s) 721, 829, 886, 1170, 1195, 1453, 2191, 2715
NmuCI GTSAC 4 cut(s) 5, 1972, 2395, 2587
NsiI ATGCAT 1 cut(s) 2631
NspI RCATGY 1 cut(s) 847
NspV TTCGAA 3 cut(s) 1294, 1443, 1987
PaeR7I CTCGAG 1 cut(s) 1372
PagI TCATGA 2 cut(s) 1152, 1216
PaqCI CACCTGC 1 cut(s) 2958
PcsI WCGNNNNNNNCGW 2 cut(s) 2349, 2454
PctI GAATGC 1 cut(s) 1390
PdmI GAANNNNTTC 1 cut(s) 1522
PfeI GAWTC 9 cut(s) 247, 350, 628, 1281, 1291, 1310, 1412, 1878, 2833
Pfl23II CGTACG 1 cut(s) 930
PinAI ACCGGT 2 cut(s) 185, 2892
Ple19I CGATCG 1 cut(s) 1061
PleI GAGTC 6 cut(s) 585, 1326, 1604, 1835, 2160, 2392
PluTI GGCGCC 1 cut(s) 723
PpsI GAGTC 6 cut(s) 585, 1326, 1604, 1835, 2160, 2392
Ppu21I YACGTR 3 cut(s) 1582, 1642, 2587
PpuMI RGGWCCY 1 cut(s) 1194
PshAI GACNNNNGTC 1 cut(s) 1053
PshBI ATTAAT 1 cut(s) 2046
Psp124BI GAGCTC 4 cut(s) 990, 1275, 1398, 2886
Psp5II RGGWCCY 1 cut(s) 1194
PspFI CCCAGC 1 cut(s) 847
PspLI CGTACG 1 cut(s) 930
PspN4I GGNNCC 8 cut(s) 721, 829, 886, 1170, 1195, 1453, 2191, 2715
PspPI GGNCC 2 cut(s) 1194, 1451
PspPPI RGGWCCY 1 cut(s) 1194
PspXI VCTCGAGB 1 cut(s) 1372
PstI CTGCAG 5 cut(s) 136, 142, 2932, 2958, 2964
PsuI RGATCY 3 cut(s) 656, 1719, 2092
PvuI CGATCG 1 cut(s) 1061
PvuII CAGCTG 4 cut(s) 137, 143, 242, 2959
RseI CAYNNNNRTG 1 cut(s) 2585
SacI GAGCTC 4 cut(s) 990, 1275, 1398, 2886
SaqAI TTAA 4 cut(s) 117, 2027, 2046, 2523
Sau96I GGNCC 2 cut(s) 1194, 1451
ScaI AGTACT 1 cut(s) 1577
SchI GAGTC 6 cut(s) 586, 1327, 1605, 1835, 2161, 2392
ScrFI CCNGG 3 cut(s) 536, 2039, 2912
SduI GDGCHC 8 cut(s) 498, 680, 990, 1275, 1344, 1398, 2716, 2886
SfaNI GCATC 5 cut(s) 235, 591, 1878, 2270, 2743
SfcI CTRYAG 7 cut(s) 132, 138, 1753, 2928, 2954, 2960, 2972
SfoI GGCGCC 1 cut(s) 721
Sfr274I CTCGAG 1 cut(s) 1372
SfuI TTCGAA 3 cut(s) 1294, 1443, 1987
SinI GGWCC 1 cut(s) 1194
SlaI CTCGAG 1 cut(s) 1372
SmiMI CAYNNNNRTG 1 cut(s) 2585
SmlI CTYRAG 1 cut(s) 1372
SmoI CTYRAG 1 cut(s) 1372
SnaBI TACGTA 1 cut(s) 1642
SspDI GGCGCC 1 cut(s) 719
SspI AATATT 1 cut(s) 160
SspMI CTAG 7 cut(s) 615, 1202, 1232, 1748, 2231, 2270, 2529
SstI GAGCTC 4 cut(s) 990, 1275, 1398, 2886
StyD4I CCNGG 3 cut(s) 534, 2037, 2910
StyI CCWWGG 4 cut(s) 637, 1266, 1416, 1431
TaiI ACGT 6 cut(s) 1304, 1584, 1644, 1974, 2589, 2820
TaqII GACCGA 2 cut(s) 1491, 2114
TatI WGTACW 3 cut(s) 272, 1575, 2481
TauI GCSGC 4 cut(s) 106, 457, 460, 720
TfiI GAWTC 9 cut(s) 247, 350, 628, 1281, 1291, 1310, 1412, 1878, 2833
Tru1I TTAA 4 cut(s) 117, 2027, 2046, 2523
Tru9I TTAA 4 cut(s) 117, 2027, 2046, 2523
TscAI CASTG 7 cut(s) 130, 1825, 1831, 1905, 2185, 2400, 2740
TseFI GTSAC 4 cut(s) 5, 1972, 2395, 2587
Tsp45I GTSAC 4 cut(s) 5, 1972, 2395, 2587
TspDTI ATGAA 8 cut(s) 885, 935, 988, 1245, 1515, 1837, 1903, 1923
TspGWI ACGGA 6 cut(s) 1197, 1265, 1459, 1586, 1658, 2040
TspRI CASTG 7 cut(s) 130, 1825, 1831, 1905, 2185, 2400, 2740
VpaK11BI GGWCC 1 cut(s) 1194
VspI ATTAAT 1 cut(s) 2046
XagI CCTNNNNNAGG 1 cut(s) 1125
XapI RAATTY 4 cut(s) 1556, 1889, 1990, 2466
XceI RCATGY 1 cut(s) 847
XhoI CTCGAG 1 cut(s) 1372
XmnI GAANNNNTTC 1 cut(s) 1522
XspI CTAG 7 cut(s) 615, 1202, 1232, 1748, 2231, 2270, 2529
ZrmI AGTACT 1 cut(s) 1577
Zsp2I ATGCAT 1 cut(s) 2631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.