Rorug01G0394000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0013224
Physical Location & Seq
Reverse (-)
50416648 .. 50431235
14588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0394000.1

Sequence Viewer

Length: 5499 bp
ATGGGCGTCCGCCACCTCACCGTGCTCGGCGAGTTCAAGCCCTTCGGCTTAATCGCCGAAGCTCTCGACGGCAAGCCCGCCGATGACGTCCCGGACAAGTACGATTACTTCCTCTTCGACCCCGACACCGTCCGAGATCGCGACGAGACCGACGACCACGACGTTCTCAGCTCCGCCTTCAGCGACCGCTGCGACCACGAGCTCTTCATTCGAGGCAATCGGATATTATGGTCAACAGGTCCACGAGTCTGTAAGAGATTCACCTTGCCTTCTCCAGTAATCAAGGCTTGCTGGTGTCGTCTGGGTGATAGGACTAAAGCTAGTCTCTGTGTATTACAAATGGCTTGTTTAACAATCTGCAACACCTCGGGTGAAGTAGTATCAATACCTGTATCGGAAACCATTACATCGATATGGCCTCTACCATTTGGTTTACTACTCCAGCAAGCAGCTCAACCGAGTTTGACTACATATGTTCCTTCGTTATCTTCAAGCCCTTTGTTTGGTGCAAGTCCTTCTGTGTCTTCTCTAAGACGAGAAGTACTGCATACGAGAAGAGAAGTTGGACTAAGTCCACAGCATAATTTCAGTTTTGTGAGTGCAATTGATCATGTTGTCAAGGAAGAAACAAGTTCAATGTCCTCACATCTAATTCTGCAAGATCCATTGGAAGAACCGCAATCGGCATATATTGAAGAAAGAGGAAAGTCAAACATAATGAAGGAATTTGATGAAAAAACTATCTGGACAAGTGATCGGATTCCCCTTATGGCATCATATAATACAGGAAAGATGCAACATTCTGTGTGGGTCGCGGAAACCAGTAATTTTAATCTTGATCTGGCAAATGCTAACTTAGTAGATGCAGTTCCTACTGATATTGTAGCAAAACACTTCTCATTTCGTAGAATATGGCAAGGGAAGGGTGCGAAGACAGCTGCTAGCAAGGTTTTCCTCGCGACTGATGACGATGCAGCTCCTATTATTTGCTTTCTTCACCAAGAACAAAAAAAGTTGCTGTCTGTAAGGCTTCAAATTGTTGAAATAAATAATGAGCTTCTCATTGACATTAAACCTGACATGAGCTGGAGCATACCTGCTGTGGCAGCCGTACCTGTAATTGTTACCCGCCCTAGAGCAAAAGTAGGACTGCTGCCATATTCGGACATGCTCATTTTGGCACCTGAAAATGCCCTTCTTCTCTATTCAGGGAAGCAATGCCTGTGTAGGTATATGTTGCCTTCTTGTTTTAGTAAAGGCCGGTTTTCACATAGTATGGAGTTTCCAGAAACATCCGTTTCCCAGGGTATGAAGATTGTGGGATTGGCTGATGCTGTTGAGGGGCGTATCAATGTTACAGTAAATAAGGGGCAGATATATCGGTGTGTATTACGAAGAAGCCCTACATCTTCTCTGGCAAATGATTGCATCACAGCTATGGCTGAGGGACTCAGTTCCAGCTTCTACAGCCATTTTCTTAGTCTTCTATGGAAAGATGGCGACTTCGCTTATTTATCAGAAGCTGATTCTAGTGTTCATTCAGAATGGGATTCTTTTCGCAGCATTATCATGGAAATGTGTCAATCAAGTACCACTTCTAAAATGATCGCGAACCCAATACCTCAGTCATCTTGGGAGTTTCTTATTAGCAGTAAATTTCACGAAATTTTTTGCAAAAATAACTTCATTGCTCAAAATTCTTCTGTAATGTCATTGGATGTACGAAGACTGGACGCCTCTCGGTTAAATACAAATAGCACCCAAAGGCTAGAGAAAACCTTTTACTCTGAGCTCTTGATGGAGAGTTTACATTGTTTGCATGCAGTATATGAAAGCCTGAAACTAAATAGTCTTCGCAAGCGGGACTTGGAGCTCCTCGGATCTCTGTTATGCTATATTGCTAGGTTTCTGGGTGAGGAAAGCTATGTTGACCATTACATTCGTGACTTTCCTGGCCTTTCCGGAAGTGTTGGGACATGTGAAATATCCTTTTCACAGGAATCTCCTCCAAGTTTATTCAGATGGCTTGAAAATTGTTTGCTATATGGACACAACTCAGCTAATACCAATGATCTTCCACCATTGATTTGTAAAGAGGGAAAGTCTGTTGTGAACTGGGCCCGGAAGATAGTGTTATTCTATAGTTTGTTAACCGGTGCCAAAGAAATAGGGAAGAAACTCTCGTCTGGTATTTACTGTAATATTGCCACGGGATCATACTGCACAAATGAGGAACTCACAGTTCTAGCAATGGTTGGGGAAAAATTTGGACTGCAACAGCTTGACTCACTGCCTTCTGGCATGTCTCTTCCTTTGCGACACGCATTGGCTAAGTGTCGGGAATCTCCTCCCACTGGTTGGCCTGCAGCTGCATATGTTCTTCTGGGCCGTGAAGATTTGGCTTTGTCATGTTTGGCACAAACATGCAATTCTAAGGAACTTGAAACTGAAACTAATGTGAATTTCATCTCTATGTCTGCACCTTACATGCTACATTTGCACCCGGTGACAATTCCTTCTGCAGTTTCTGATACAAATGGATTGGACAATACTAAGTTTGAGGACACAGATTCGATTGACGGGTCTATGACAGATGGTATGGAGCATATCTTCAACTCAAGCACACAGTTGCGATATGGTCGTGATTTGCGACTGAATGAGGTTAGACGGCTCCTGTGCTCTGCAAGACCCGTGGCAATTCAAACATCTGTTAATCCTAGTGCGTCTGATCAGGACCTCCAACAGGCCCAGCTATGGCACCTTGCACAAAGGACTACTGCCCTTCCTCTTGGCCGTGGAGCATTCACTCTTGCAACCATATATACACTTCTGACAGAGGCCTTTGTAGTCCCGAAGATTGTATTAGCAGGTCGATTGCCTGCCCAGCAAAATGCAACTGTTAATCTGGACCCGAATGTGAGGAATGTAGAAGAAATTAAAACTTGGCCTGAATTCCACAATGCTGTTGCTGCTGGACTAAGGCTGGCTCCTCTTCAGGGAAAGATGTCAAAGACGTGGATCAAATATAATAAACCAGAGGAGCCAAATGCTGTTCATGCCGGACTTCTCTTTGCTTTAGGATTACATGGTTACCTACGTGTTTTGAATATTACTGACATATTTCAGTATTTTCGACAGGAACATGAAATCACAACTCTAGGACTAATGCTTGGCCTGGCAGCTACCTATAGAGGGACTATGCAACCAGAAATATCTAAGTGTCTTTTTGTTCATATACCTGCTCGACATCCATCTTCATTTCCAGAATTGGAGTTACCAACGCTTCTACAGTCAGCAGCTCTAATGTCAGTTGGGCTTCTTTATGAAGGATCAGCGCACGCACAAACAATGCAGATTCTCTTGGGGGAAATAGGACGTAGAACTGCAGGTGATAATGTACTAGAAAGGGAAGGTTATGCTGTTTCTGCAGGATTTTCCCTGGGTCTTCTTGCATTGGGTCGGGGAGAAGATGCTCTCGGTTTCATGGAGACAGTGGTCGACAAATTGTTCCATTATATTGGTGGAAAAGAAGTTAATGATAGATCCATTTCATCAACACTATCAGTAGATGACAATCGTGCTGCTGCGCAGATGATGGAAGGGACTGCAGTGAATGTTGATGTCACAGCACCTGGAGCCACAATTGCTCTTGCTTTAATGTTTTTAAGGACCGAATCACAGGCAATTGTGTCTAAGCTCTCTATTCCTCGTACACAATTTGAGCTGCAATATGTGAGGCCTGATTTCATTATGCTTCGCGTCATTGCTCGAAATTTGATAATGTGGAGCAGGATTCATCCTTCCCAAGATTGGGTACAGTCTCAGATTCCGGATATTATCAAAAATGGTGTCAAATTCCTTGGGGATGATACTGATGATATTGATGAAATGGATGCTGAATCATTTGTTCAGGCATATGTCAATATAGTGGCTGGAGCCTGCATTTCTCTTGGGTTGAGATTCGCTGGTACAAAGGATGGGCACGCACAGGAGTTACTTTACAACTATGCTGTTTACTTTTTAAACGAGATCAAGCCAGTTTCTGCTACAAGTGGAAGCTTCCCAAAGGGATTGTCTCGCTATGTTGATCGGGGGACTCTGGAAATATGCCTGCATCTCATTGTTCTTTCCTTGTGTGTGGTAATGGCTGGTTCTGGGCATTTACAAACCTTTAAGCTATTGAGATTTCTCCGCAGCAGAAATTCTGCTGATGGGCATGTTAACTATGGCGTTCAGATGGCTGTAAGCCTAGCAATTGGTTTCTTGTTCCTTGGTGGAGGAATGCAGACATTTTCAACAAGCAACAGTTCAGTTGCCGCTTTGCTGATTACTCTTTACCCACGCTTGCCAACTGGACCAAATGATAATAGATGCCATTTCCAGGCATTTCGACATTTGTACGTACTGGCAACGGAAGCTCGCTGGATCCAGACTGTTGATGTTGACACTGGTCTACCAGTGTATGCCCCCCTTGAAGTTACTATAAGGGAAACTGAGCATTATGCAGAAACAAGTTTTTGTGAGGTTACACCGTGCCTTCTGCCAGAGCGTGCCATTTTGAAGACACTACGTATTTGTGGTCCTCGGTACTGGCCTCAAGTCATAGATCTCGTCCCTGAAGATAAACCTTGGTGGACCTCTGGGGACAAGAATAGCCCATTTAATTCTGGTGTTTTGTATATCAAGAGAAAGGTTGGAGCTTGTTCATATATTGATGATCCCGTAGGGTGTCAGTCGTTGCTGTCACGTGCAATGCATAAGGTGTTTGGTTTGACAAGTTTAAAAGCACCTAGCCCATCCTCTAGTGGCCAACATGGACTTGGTTCAGTTACAGTTGATCAGTTGGTTGGTACCTTCTCCTCCAATCCTAGCTTAATTGCATTTGCACAACTCTGCTGTGACCCTTCCTGGAAGAGCAGATCTGATATTGACTTCCAGGAATTTTGCTTGCAAGTGTTATTTGAGTGTGTGAGCAAGGACAGGCCTGCTCTTTTACAGGTCTATTTGTCATTATACACGACAATGGGGTCAATGGCAGGTCAAGTAACTAGTGATACTGTTGTTTTATGTGACTCGTTATCTATCTCCAATCTTAAGCTTGCACTAGCATACAATGAGGCTTTGTTGAGTGGAAGATTGACCTCTTCGAGAGGTGGCATTGTTCAATCCAACTTTATAGCATGTCTTCGGAAGCAGGTGGAAGCACTCCTAGATTGTTCTCAGGACTTGAAGGATGATTTTTGTAATTATATGTATTCCGGGAAGTGGCCAAATGCGGAAGAATCCCAGGGAAATAAACGTTCAATACTTCTTTCTTGGTACCTTCAATGGTTTTGTGTACCAGCACCATATGCAATTAAGACAGCTGTGGACAAAGTCAAGCCCAAGCTCAAGTCATCCTCATCTTTGGTCCCTTTTCTGCGGTTATTATTTCCGAAAGCTCATGTCAATGCTATTGCTGAGATAGATAAGCTCTTCTCATCCACTAATGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1832

Amino Acids

202.83

Weight (kDa)

6.2

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC1 PF12859 31 - 274 7.8e-28 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
ANAPC1 PF12859 303 - 522 1.1e-31 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
Apc1_MidN PF20518 543 - 801 6.9e-19 Anaphase-promoting complex subunit 1 middle domain
APC1_3rd PF21282 1474 - 1537 1.8e-15 APC1 beta sandwich domain
APC1_C PF18122 1614 - 1781 5.3e-33 Anaphase-promoting complex sub unit 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51800
fragaria_vesca FvH4_7g28640 FvH4_7g28640
malus_domestica MD00G1003400.v1.1 MD01G1192000.v1.1
prunus_persica Prupe.2G286400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0376121
rosa_laevigata RLG00000026631
rosa_multiflora Rmu_ssc0000116.1_g000035
rosa_roxburghii Rroxscaffold_4G00282390
rosa_rugosa Rorug01G0393900 Rorug01G0394000
rosa_samantha Rh1AG405300 Rh1BG368900 Rh1BG369000 Rh1CG381900 Rh1DG398800
rosa_wichuraiana Rw1G035970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 3377, 5185
AasI GACNNNNNNGTC 1 cut(s) 5026
AatII GACGTC 1 cut(s) 90
Acc16I TGCGCA 1 cut(s) 3588
Acc36I ACCTGC 6 cut(s) 1105, 2858, 3247, 3377, 5027, 5185
Acc65I GGTACC 2 cut(s) 4850, 5319
AccB1I GGYRCC 5 cut(s) 1180, 2156, 2757, 4850, 5319
AccB7I CCANNNNNTGG 1 cut(s) 2358
AccI GTMKAC 2 cut(s) 3498, 4453
AccII CGCG 5 cut(s) 141, 815, 959, 1610, 3759
AccIII TCCGGA 2 cut(s) 1961, 3829
AclI AACGTT 1 cut(s) 5299
AclWI GGATC 9 cut(s) 656, 1888, 2221, 3026, 3337, 3537, 4420, 4433, 4712
AcoI YGGCCR 3 cut(s) 2791, 4807, 5267
AcuI CTGAAG 3 cut(s) 163, 2978, 4638
AcvI CACGTG 1 cut(s) 4748
AcyI GRCGYC 3 cut(s) 6, 87, 1734
AdeI CACNNNGTG 1 cut(s) 2506
AflII CTTAAG 1 cut(s) 5093
AflIII ACRYGT 2 cut(s) 1976, 3097
AgeI ACCGGT 1 cut(s) 2153
AhlI ACTAGT 1 cut(s) 5048
AjiI CACGTC 1 cut(s) 3015
AjnI CCWGG 9 cut(s) 1302, 1951, 3174, 3438, 3631, 4380, 4907, 4935, 5286
Alw21I GWGCWC 5 cut(s) 27, 204, 1794, 1875, 2681
Alw26I GTCTC 6 cut(s) 140, 329, 2310, 3482, 3825, 4080
AlwI GGATC 9 cut(s) 656, 1888, 2221, 3026, 3337, 3537, 4420, 4433, 4712
AlwNI CAGNNNCTG 4 cut(s) 1523, 2528, 3632, 4043
Ama87I CYCGRG 1 cut(s) 367
Aor13HI TCCGGA 2 cut(s) 1961, 3829
ApaI GGGCCC 1 cut(s) 2122
ArsI GACNNNNNNTTYG 4 cut(s) 3834, 3866, 4058, 4090
AsiGI ACCGGT 1 cut(s) 2153
Asp718I GGTACC 2 cut(s) 4850, 5319
AspLEI GCGC 2 cut(s) 3337, 3589
AsuC2I CCSGG 4 cut(s) 92, 2122, 2504, 5260
AsuHPI GGTGA 8 cut(s) 10, 253, 317, 383, 989, 1925, 2518, 3401
AsuNHI GCTAGC 1 cut(s) 941
AvaI CYCGRG 1 cut(s) 367
AvaII GGWCC 8 cut(s) 239, 2734, 2908, 3669, 4355, 4580, 4635, 5410
BaeGI GKGCMC 2 cut(s) 2122, 3984
BaeI ACNNNNGTAYC 2 cut(s) 5046, 5079
BalI TGGCCA 2 cut(s) 4809, 5269
BamHI GGATCC 1 cut(s) 4425
BanI GGYRCC 5 cut(s) 1180, 2156, 2757, 4850, 5319
BanII GRGCYC 4 cut(s) 204, 1794, 1875, 2122
BarI GAAGNNNNNNTAC 4 cut(s) 92, 124, 1387, 1419
BauI CACGAG 2 cut(s) 197, 243
BbrPI CACGTG 1 cut(s) 4748
BbsI GAAGAC 8 cut(s) 516, 938, 1475, 1732, 1844, 3437, 4568, 5177
Bbv12I GWGCWC 5 cut(s) 27, 204, 1794, 1875, 2681
BbvCI CCTCAGC 1 cut(s) 1443
BceAI ACGGC 5 cut(s) 85, 1094, 2373, 2684, 2778
BcgI CGANNNNNNTGC 2 cut(s) 1361, 1395
BciT130I CCWGG 9 cut(s) 1304, 1953, 3176, 3440, 3633, 4382, 4909, 4937, 5288
BclI TGATCA 3 cut(s) 607, 2728, 4837
BcnI CCSGG 4 cut(s) 92, 2122, 2504, 5260
BcoDI GTCTC 6 cut(s) 140, 329, 2310, 3482, 3825, 4080
BcuI ACTAGT 1 cut(s) 5048
BfmI CTRYAG 9 cut(s) 1465, 2140, 2364, 2520, 3187, 3287, 3384, 3426, 3606
BfrI CTTAAG 1 cut(s) 5093
BfuAI ACCTGC 6 cut(s) 1105, 2858, 3247, 3377, 5027, 5185
BglII AGATCT 2 cut(s) 4606, 4919
BmcAI AGTACT 1 cut(s) 543
Bme18I GGWCC 8 cut(s) 239, 2734, 2908, 3669, 4355, 4580, 4635, 5410
BmeT110I CYCGRG 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 3015
BmrI ACTGGG 1 cut(s) 2125
BmtI GCTAGC 1 cut(s) 945
BmuI ACTGGG 1 cut(s) 2125
BoxI GACNNNNGTC 2 cut(s) 3494, 4449
BpiI GAAGAC 8 cut(s) 516, 938, 1475, 1732, 1844, 3437, 4568, 5177
BpmI CTGGAG 5 cut(s) 258, 425, 1108, 3654, 3954
Bpu10I CCTNAGC 1 cut(s) 1443
BpuEI CTTGAG 3 cut(s) 2602, 4581, 5375
BpuMI CCSGG 4 cut(s) 92, 2122, 2504, 5260
Bsa29I ATCGAT 1 cut(s) 410
BsaAI YACGTR 4 cut(s) 3098, 4402, 4571, 4748
BsaBI GATNNNNATC 1 cut(s) 3573
BsaHI GRCGYC 3 cut(s) 6, 87, 1734
BsaI GGTCTC 1 cut(s) 140
BsaWI WCCGGW 3 cut(s) 1961, 2153, 3829
Bse118I RCCGGY 2 cut(s) 1260, 2153
Bse3DI GCAATG 5 cut(s) 1223, 1686, 2256, 3762, 4758
Bse8I GATNNNNATC 1 cut(s) 3573
BseAI TCCGGA 2 cut(s) 1961, 3829
BseBI CCWGG 9 cut(s) 1304, 1953, 3176, 3440, 3633, 4382, 4909, 4937, 5288
BseCI ATCGAT 1 cut(s) 410
BseJI GATNNNNATC 1 cut(s) 3573
BseMI GCAATG 5 cut(s) 1223, 1686, 2256, 3762, 4758
BseRI GAGGAG 6 cut(s) 1865, 1995, 2337, 2979, 3053, 4849
BseSI GKGCMC 2 cut(s) 2122, 3984
BseYI CCCAGC 2 cut(s) 2748, 2883
BsgI GTGCAG 2 cut(s) 2206, 2463
Bsh1236I CGCG 5 cut(s) 141, 815, 959, 1610, 3759
Bsh1285I CGRYCG 1 cut(s) 187
BshNI GGYRCC 5 cut(s) 1180, 2156, 2757, 4850, 5319
BshTI ACCGGT 1 cut(s) 2153
BshVI ATCGAT 1 cut(s) 410
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 5 cut(s) 27, 204, 1794, 1875, 2681
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 9 cut(s) 92, 1261, 1962, 2122, 2154, 2504, 3060, 3830, 5259
BsmAI GTCTC 6 cut(s) 140, 329, 2310, 3482, 3825, 4080
BsmI GAATGC 2 cut(s) 2801, 4287
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 367
Bsp120I GGGCCC 1 cut(s) 2118
Bsp1286I GDGCHC 7 cut(s) 27, 204, 1794, 1875, 2122, 2681, 3984
Bsp13I TCCGGA 2 cut(s) 1961, 3829
Bsp68I TCGCGA 3 cut(s) 141, 959, 1610
BspDI ATCGAT 1 cut(s) 410
BspEI TCCGGA 2 cut(s) 1961, 3829
BspFNI CGCG 5 cut(s) 141, 815, 959, 1610, 3759
BspMAI CTGCAG 5 cut(s) 2368, 2524, 3388, 3430, 3610
BspMI ACCTGC 6 cut(s) 1105, 2858, 3247, 3377, 5027, 5185
BspOI GCTAGC 1 cut(s) 945
BspPI GGATC 9 cut(s) 656, 1888, 2221, 3026, 3337, 3537, 4420, 4433, 4712
BspQI GCTCTTC 3 cut(s) 209, 4907, 5480
BspT107I GGYRCC 5 cut(s) 1180, 2156, 2757, 4850, 5319
BspTI CTTAAG 1 cut(s) 5093
BspTNI GGTCTC 1 cut(s) 140
BsrDI GCAATG 5 cut(s) 1223, 1686, 2256, 3762, 4758
BsrFI RCCGGY 2 cut(s) 1260, 2153
BssAI RCCGGY 2 cut(s) 1260, 2153
BssNI GRCGYC 3 cut(s) 6, 87, 1734
BssSI CACGAG 2 cut(s) 197, 243
BssT1I CCWWGG 3 cut(s) 3859, 4270, 4628
Bst2BI CACGAG 2 cut(s) 197, 243
Bst2UI CCWGG 9 cut(s) 1304, 1953, 3176, 3440, 3633, 4382, 4909, 4937, 5288
Bst6I CTCTTC 8 cut(s) 119, 209, 550, 2313, 2997, 4907, 5149, 5480
BstACI GRCGYC 3 cut(s) 6, 87, 1734
BstAFI CTTAAG 1 cut(s) 5093
BstAPI GCANNNNNTGC 1 cut(s) 5351
BstBAI YACGTR 4 cut(s) 3098, 4402, 4571, 4748
BstDSI CCRYGG 3 cut(s) 2208, 2691, 2794
BstEII GGTNACC 1 cut(s) 3089
BstENI CCTNNNNNAGG 1 cut(s) 2741
BstFNI CGCG 5 cut(s) 141, 815, 959, 1610, 3759
BstHHI GCGC 2 cut(s) 3337, 3589
BstMAI GTCTC 6 cut(s) 140, 329, 2310, 3482, 3825, 4080
BstMCI CGRYCG 1 cut(s) 187
BstNI CCWGG 9 cut(s) 1304, 1953, 3176, 3440, 3633, 4382, 4909, 4937, 5288
BstNSI RCATGY 8 cut(s) 1171, 1823, 1980, 2305, 2427, 2491, 4220, 5184
BstPAI GACNNNNGTC 2 cut(s) 3494, 4449
BstPI GGTNACC 1 cut(s) 3089
BstSFI CTRYAG 9 cut(s) 1465, 2140, 2364, 2520, 3187, 3287, 3384, 3426, 3606
BstSLI GKGCMC 2 cut(s) 2122, 3984
BstSNI TACGTA 2 cut(s) 4402, 4571
BstUI CGCG 5 cut(s) 141, 815, 959, 1610, 3759
BstV2I GAAGAC 8 cut(s) 516, 938, 1475, 1732, 1844, 3437, 4568, 5177
BstX2I RGATCY 6 cut(s) 661, 1880, 3542, 4425, 4606, 4919
BstXI CCANNNNNNTGG 1 cut(s) 3518
BstYI RGATCY 6 cut(s) 661, 1880, 3542, 4425, 4606, 4919
Bsu15I ATCGAT 1 cut(s) 410
BsuTUI ATCGAT 1 cut(s) 410
BtgI CCRYGG 3 cut(s) 2208, 2691, 2794
BtrI CACGTC 1 cut(s) 3015
BtsI GCAGTG 2 cut(s) 2288, 3615
BtsIMutI CAGTG 6 cut(s) 2288, 2352, 3498, 3615, 4446, 4464
BtuMI TCGCGA 3 cut(s) 141, 959, 1610
BveI ACCTGC 6 cut(s) 1105, 2858, 3247, 3377, 5027, 5185
CaiI CAGNNNCTG 4 cut(s) 1523, 2528, 3632, 4043
CfoI GCGC 2 cut(s) 3337, 3589
Cfr10I RCCGGY 2 cut(s) 1260, 2153
ClaI ATCGAT 1 cut(s) 410
CseI GACGC 3 cut(s) 1742, 2712, 3748
CspAI ACCGGT 1 cut(s) 2153
CspCI CAANNNNNGTGG 2 cut(s) 2673, 2708
DraI TTTAAA 2 cut(s) 4023, 4782
DraIII CACNNNGTG 1 cut(s) 2506
DrdI GACNNNNNNGTC 1 cut(s) 5026
DseDI GACNNNNNNGTC 1 cut(s) 5026
EaeI YGGCCR 3 cut(s) 2791, 4807, 5267
Eam1104I CTCTTC 8 cut(s) 119, 209, 550, 2313, 2997, 4907, 5149, 5480
EarI CTCTTC 8 cut(s) 119, 209, 550, 2313, 2997, 4907, 5149, 5480
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 3 cut(s) 202, 1792, 1873
Eco105I TACGTA 2 cut(s) 4402, 4571
Eco130I CCWWGG 3 cut(s) 3859, 4270, 4628
Eco147I AGGCCT 3 cut(s) 2840, 3739, 4984
Eco24I GRGCYC 4 cut(s) 204, 1794, 1875, 2122
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 8 cut(s) 239, 2734, 2908, 3669, 4355, 4580, 4635, 5410
Eco53kI GAGCTC 3 cut(s) 202, 1792, 1873
Eco57I CTGAAG 3 cut(s) 163, 2978, 4638
Eco72I CACGTG 1 cut(s) 4748
Eco88I CYCGRG 1 cut(s) 367
Eco91I GGTNACC 1 cut(s) 3089
EcoICRI GAGCTC 3 cut(s) 202, 1792, 1873
EcoNI CCTNNNNNAGG 1 cut(s) 2741
EcoO109I RGGNCCY 1 cut(s) 2734
EcoO65I GGTNACC 1 cut(s) 3089
EcoRI GAATTC 1 cut(s) 2951
EcoRII CCWGG 9 cut(s) 1302, 1951, 3174, 3438, 3631, 4380, 4907, 4935, 5286
EcoT14I CCWWGG 3 cut(s) 3859, 4270, 4628
EcoT22I ATGCAT 1 cut(s) 4758
EcoT38I GRGCYC 4 cut(s) 204, 1794, 1875, 2122
ErhI CCWWGG 3 cut(s) 3859, 4270, 4628
FalI AAGNNNNNCTT 4 cut(s) 1579, 1611, 1850, 1882
FauI CCCGC 3 cut(s) 85, 1136, 1854
FauNDI CATATG 4 cut(s) 472, 2374, 3916, 5350
FbaI TGATCA 3 cut(s) 607, 2728, 4837
FblI GTMKAC 2 cut(s) 3498, 4453
FriOI GRGCYC 4 cut(s) 204, 1794, 1875, 2122
FspI TGCGCA 1 cut(s) 3588
GlaI GCGC 2 cut(s) 3336, 3588
GsaI CCCAGC 2 cut(s) 2752, 2887
GsuI CTGGAG 5 cut(s) 258, 425, 1108, 3654, 3954
HapII CCGG 9 cut(s) 92, 1261, 1962, 2122, 2154, 2504, 3060, 3830, 5259
HgaI GACGC 3 cut(s) 1742, 2712, 3748
HhaI GCGC 2 cut(s) 3337, 3589
Hin1I GRCGYC 3 cut(s) 6, 87, 1734
Hin6I GCGC 2 cut(s) 3335, 3587
HinP1I GCGC 2 cut(s) 3335, 3587
HincII GTYRAC 6 cut(s) 234, 1930, 2151, 3499, 4222, 4444
HindII GTYRAC 6 cut(s) 234, 1930, 2151, 3499, 4222, 4444
HindIII AAGCTT 2 cut(s) 4057, 5096
HpaI GTTAAC 2 cut(s) 2151, 4222
HpaII CCGG 9 cut(s) 92, 1261, 1962, 2122, 2154, 2504, 3060, 3830, 5259
HphI GGTGA 8 cut(s) 10, 253, 317, 383, 989, 1925, 2518, 3401
Hpy99I CGWCG 4 cut(s) 71, 146, 155, 164
HpyCH4IV ACGT 9 cut(s) 87, 162, 3014, 3097, 3376, 4401, 4570, 4747, 5299
HpySE526I ACGT 9 cut(s) 87, 162, 3014, 3097, 3376, 4401, 4570, 4747, 5299
Hsp92I GRCGYC 3 cut(s) 6, 87, 1734
HspAI GCGC 2 cut(s) 3335, 3587
Kpn2I TCCGGA 2 cut(s) 1961, 3829
KpnI GGTACC 2 cut(s) 4854, 5323
Ksp22I TGATCA 3 cut(s) 607, 2728, 4837
KspAI GTTAAC 2 cut(s) 2151, 4222
LguI GCTCTTC 3 cut(s) 209, 4907, 5480
MaeII ACGT 9 cut(s) 87, 162, 3014, 3097, 3376, 4401, 4570, 4747, 5299
MfeI CAATTG 4 cut(s) 603, 3642, 3684, 4254
MflI RGATCY 6 cut(s) 661, 1880, 3542, 4425, 4606, 4919
MhlI GDGCHC 7 cut(s) 27, 204, 1794, 1875, 2122, 2681, 3984
MlsI TGGCCA 2 cut(s) 4809, 5269
MluNI TGGCCA 2 cut(s) 4809, 5269
MlyI GAGTC 5 cut(s) 255, 1443, 2279, 4090, 5066
MmeI TCCRAC 4 cut(s) 544, 2764, 4675, 5193
Mox20I TGGCCA 2 cut(s) 4809, 5269
Mph1103I ATGCAT 1 cut(s) 4758
MroI TCCGGA 2 cut(s) 1961, 3829
MscI TGGCCA 2 cut(s) 4809, 5269
MslI CAYNNNNRTG 8 cut(s) 412, 1436, 1568, 1574, 2422, 2471, 5021, 5448
Msp20I TGGCCA 2 cut(s) 4809, 5269
MspA1I CMGCKG 4 cut(s) 189, 938, 2369, 5366
MspCI CTTAAG 1 cut(s) 5093
MspI CCGG 9 cut(s) 92, 1261, 1962, 2122, 2154, 2504, 3060, 3830, 5259
MunI CAATTG 4 cut(s) 603, 3642, 3684, 4254
Mva1269I GAATGC 2 cut(s) 2801, 4287
MvaI CCWGG 9 cut(s) 1304, 1953, 3176, 3440, 3633, 4382, 4909, 4937, 5288
MvnI CGCG 5 cut(s) 141, 815, 959, 1610, 3759
NciI CCSGG 4 cut(s) 92, 2122, 2504, 5260
NdeI CATATG 4 cut(s) 472, 2374, 3916, 5350
NheI GCTAGC 1 cut(s) 941
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 6 cut(s) 1943, 2506, 3622, 4743, 4898, 5069
NruI TCGCGA 3 cut(s) 141, 959, 1610
NsbI TGCGCA 1 cut(s) 3588
NsiI ATGCAT 1 cut(s) 4758
NspI RCATGY 8 cut(s) 1171, 1823, 1980, 2305, 2427, 2491, 4220, 5184
PaeI GCATGC 1 cut(s) 1823
PaqCI CACCTGC 2 cut(s) 3377, 5185
PasI CCCWGGG 3 cut(s) 1303, 3439, 5287
PceI AGGCCT 3 cut(s) 2840, 3739, 4984
PciI ACATGT 1 cut(s) 1976
PciSI GCTCTTC 3 cut(s) 209, 4907, 5480
PcsI WCGNNNNNNNCGW 3 cut(s) 150, 159, 2647
PctI GAATGC 2 cut(s) 2801, 4287
PflFI GACNNNGTC 3 cut(s) 128, 570, 5375
PflMI CCANNNNNTGG 1 cut(s) 2358
PfoI TCCNGGA 4 cut(s) 90, 4907, 4935, 5258
PinAI ACCGGT 1 cut(s) 2153
PleI GAGTC 5 cut(s) 254, 1443, 2279, 4090, 5066
PmaCI CACGTG 1 cut(s) 4748
PmlI CACGTG 1 cut(s) 4748
PpsI GAGTC 5 cut(s) 254, 1443, 2279, 4090, 5066
Ppu21I YACGTR 4 cut(s) 3098, 4402, 4571, 4748
PpuMI RGGWCCY 1 cut(s) 2734
PscI ACATGT 1 cut(s) 1976
PshAI GACNNNNGTC 2 cut(s) 3494, 4449
Psp124BI GAGCTC 3 cut(s) 204, 1794, 1875
Psp1406I AACGTT 1 cut(s) 5299
Psp5II RGGWCCY 1 cut(s) 2734
Psp6I CCWGG 9 cut(s) 1302, 1951, 3174, 3438, 3631, 4380, 4907, 4935, 5286
PspCI CACGTG 1 cut(s) 4748
PspEI GGTNACC 1 cut(s) 3089
PspFI CCCAGC 2 cut(s) 2748, 2883
PspGI CCWGG 9 cut(s) 1302, 1951, 3174, 3438, 3631, 4380, 4907, 4935, 5286
PspOMI GGGCCC 1 cut(s) 2118
PspPPI RGGWCCY 1 cut(s) 2734
PsrI GAACNNNNNNTAC 2 cut(s) 852, 884
PstI CTGCAG 5 cut(s) 2368, 2524, 3388, 3430, 3610
PstNI CAGNNNCTG 4 cut(s) 1523, 2528, 3632, 4043
PsuI RGATCY 6 cut(s) 661, 1880, 3542, 4425, 4606, 4919
PsyI GACNNNGTC 3 cut(s) 128, 570, 5375
PvuII CAGCTG 3 cut(s) 938, 2369, 5366
RruI TCGCGA 3 cut(s) 141, 959, 1610
RseI CAYNNNNRTG 8 cut(s) 412, 1436, 1568, 1574, 2422, 2471, 5021, 5448
SacI GAGCTC 3 cut(s) 204, 1794, 1875
SalI GTCGAC 1 cut(s) 3497
SapI GCTCTTC 3 cut(s) 209, 4907, 5480
ScaI AGTACT 1 cut(s) 543
SchI GAGTC 5 cut(s) 255, 1443, 2279, 4090, 5066
SduI GDGCHC 7 cut(s) 27, 204, 1794, 1875, 2122, 2681, 3984
SfcI CTRYAG 9 cut(s) 1465, 2140, 2364, 2520, 3187, 3287, 3384, 3426, 3606
SinI GGWCC 8 cut(s) 239, 2734, 2908, 3669, 4355, 4580, 4635, 5410
SmiMI CAYNNNNRTG 8 cut(s) 412, 1436, 1568, 1574, 2422, 2471, 5021, 5448
SmlI CTYRAG 4 cut(s) 2617, 4596, 5093, 5390
SmoI CTYRAG 4 cut(s) 2617, 4596, 5093, 5390
SnaBI TACGTA 2 cut(s) 4402, 4571
SpeI ACTAGT 1 cut(s) 5048
SphI GCATGC 1 cut(s) 1823
SseBI AGGCCT 3 cut(s) 2840, 3739, 4984
SspI AATATT 2 cut(s) 2203, 3109
SstI GAGCTC 3 cut(s) 204, 1794, 1875
StuI AGGCCT 3 cut(s) 2840, 3739, 4984
StyI CCWWGG 3 cut(s) 3859, 4270, 4628
TaiI ACGT 9 cut(s) 90, 165, 3017, 3100, 3379, 4404, 4573, 4750, 5302
TaqII GACCGA 2 cut(s) 164, 3686
TatI WGTACW 2 cut(s) 541, 3397
TauI GCSGC 1 cut(s) 4319
TscAI CASTG 6 cut(s) 2295, 2359, 3498, 3615, 4453, 4464
TseFI GTSAC 6 cut(s) 1943, 2506, 3622, 4743, 4898, 5069
Tsp45I GTSAC 6 cut(s) 1943, 2506, 3622, 4743, 4898, 5069
TspGWI ACGGA 2 cut(s) 1285, 4427
TspRI CASTG 6 cut(s) 2295, 2359, 3498, 3615, 4453, 4464
Tth111I GACNNNGTC 3 cut(s) 128, 570, 5375
Van91I CCANNNNNTGG 1 cut(s) 2358
Vha464I CTTAAG 1 cut(s) 5093
VpaK11BI GGWCC 8 cut(s) 239, 2734, 2908, 3669, 4355, 4580, 4635, 5410
XagI CCTNNNNNAGG 1 cut(s) 2741
XceI RCATGY 8 cut(s) 1171, 1823, 1980, 2305, 2427, 2491, 4220, 5184
XcmI CCANNNNNNNNNTGG 2 cut(s) 3518, 4817
XmiI GTMKAC 2 cut(s) 3498, 4453
ZraI GACGTC 1 cut(s) 88
ZrmI AGTACT 1 cut(s) 543
Zsp2I ATGCAT 1 cut(s) 4758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.