Rmu_ssc0000116.1_g000035

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000116.1
OG0013224
Physical Location & Seq
Reverse (-)
164815 .. 168163
3349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000116.1_g000035.1.cds

Sequence Viewer

Length: 2928 bp
atgggtgaaaccaaaggagataaccccaataagcagcaacaacaaccacaacaaatttcagcctctccaaatgacccccttgaagaagcatctccattaaccgtagttgtgaccggagctactgctccctttaacattccggctccattatatgttccaatcagtggtgcaacatcttcttcattaccctttgatcaacaacaatttgaggcggtgaatccgaagagacctagatacaccagtaccggccaatggaagcttctaccatctccgtcttcccaacagaaacagactcctacccaaaccccgtccccgaaccccaaaacccatccccaatcacacactactaccggagcttcttcctcggatacagcctcatccccacctcactctcctctcccgtctctctccgcaacctcaggccaagacacaaccaaaacagaaggagaagaacaaaacccatctcttcaccgcagtcaattccgaagaggcaagtatgtcagcccggtttggaagccccacgagatgttgtggctagccaaggcttggagggctgtttatcaaagccaaggctcagacgggtccggttcatcttcaagaacggaacaccactcagatataactggtcaacccaccagagccaaaacaagggctgataaggacaaggaagtagccgagtttcttcagaagaatgggatcaaccgagacgcgaaaacagccggtacaaaatgggataacatgctgggtgagtttagaaaagtctatgagtgggaaagaggagctgagagagaacaaattgggaacaagagctattttaggctttctccttacgagaggaagcttcacagattgcctgcttcttttgatgaagaggtatttgatgagctcgcacagtttatggggtccagaatgaggactcctccgatcagcagaaccagtgatagcacacggactcaatctctttatgtcaccacaagtactattttgccgcaaccaccttctttcagagacgacgacctccctctctcaggtagggcaaggcagctgatgatcatgagcggtggtggggaagtacctttttaccccagtggaagatcggggagcctgttagggtttgatcatctacctccctatcatcaccaatcttcactagactacgctgcaggtgtttcttcatcgacttcatctttgacgaaggagcttcgtcgaattgggaagattaggatgacatgggaagaatccgttagtttgtgggctgaagaaggtgagcaccaccgtgggagggtcaagattcaaggcggctccagcttcttgaatgcggacgagctcgctttctttgatgattccatggttgcctgcaccatggaagcttttgaagatggcactcttagaggtttctctgttgaccgttttgtttctggtcaacaagtcaaggtctttggcagacgaaagccatccaccccagcaatgcctccatgggaatttcaagacccaagtgagtactacattacttgtcttcgagttccacccccatcgcttccacgcttatccgagctttcaagctacttactacagccaccgcccgaggagctccgatttccactccgaaaagacgtttaccgggacttgccacaggggagagagactttcttcactgcatcaaatgattcgctggattgtagagccatcacgtacgatattgtaagccccattattcgaagcaactatggtcttagtgctgccaccagtagagactccttcattggcctttgggatgactgcatcaataggttcgtgtccaagttttcttgcgtcgacatggtcttcattcgaaaggctacttcttcaatatcaataactgaagctttgcaagatcaatggccaaatgtgacagggttcgtgaaaaactattgtttgtggagaggggaggagattgatcaagtaagagacagtcacattcatccttccaaatcattagcggagaaacttcaatggacatataatgatcttccttacattttcgggtactactctataggtaatatggtcacattttgtgcgttaagtaaaggaggacaagatcgaattattgagactaatttacacacgattgatctatctttgccatcggatagactcaaagccctagtcctttgttacagagttgctgggttattgccattgctagccgatagatgcttcaacataaacagtgggactactagtactagcaatggggccgctgcaagttacaagtttatgttcggtccttatagtgattttgagagggttgatttgggtaatggaaatattgttgagttgaccccaaatacagtgaccagatttttctccaaccaaaggaagtggttagcggtaaaagaaatctacgacttccttgatcacagaataccccatgcagagtacattcacaggtcctcggagaaagacttggccttggttttcaagccaagagggtgcaagttcaagccaagaaactgcgacgagctcgtggaggcgctcaagtacgtgaccaaagctctggtggcgctacacgacttgtcgttcatgcacagggacatatgctgggacaaggtgatgaggagcacggagagagacaacgagtggctcctttgtgggttcgacgaggcggttggggcaccacagttgaatccgtaccaggttgctgcggcggagcgtggcgaacacgcaccggagctggagagggcgttgcatggggtgaaagtggacgtgtggggtgtgggtcatctggtgaggacttgtgggttggcaggtgtgccgaagatgctgagggagctccagaatcggtgtttggaccaaaacccagagcttaggccaactgcagccgactgctaccaccacctgcttcagctgcagtcgtcactgtcagttgtcaccgctatgatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

975

Amino Acids

109.46

Weight (kDa)

6.83

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51800
fragaria_vesca FvH4_7g28640 FvH4_7g28640
malus_domestica MD00G1003400.v1.1 MD01G1192000.v1.1
prunus_persica Prupe.2G286400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0376121
rosa_laevigata RLG00000026631
rosa_multiflora Rmu_ssc0000116.1_g000035
rosa_roxburghii Rroxscaffold_4G00282390
rosa_rugosa Rorug01G0393900 Rorug01G0394000
rosa_samantha Rh1AG405300 Rh1BG368900 Rh1BG369000 Rh1CG381900 Rh1DG398800
rosa_wichuraiana Rw1G035970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1154, 2780, 2889
Acc36I ACCTGC 3 cut(s) 1154, 2780, 2889
AccB1I GGYRCC 1 cut(s) 2656
AccB7I CCANNNNNTGG 2 cut(s) 164, 546
AccBSI CCGCTC 1 cut(s) 1059
AccI GTMKAC 1 cut(s) 1815
AccII CGCG 1 cut(s) 710
AclWI GGATC 1 cut(s) 704
AcoI YGGCCR 2 cut(s) 247, 1880
AcsI RAATTY 2 cut(s) 54, 1481
AcuI CTGAAG 4 cut(s) 668, 1278, 1881, 2870
AfiI CCNNNNNNNGG 8 cut(s) 164, 252, 546, 648, 912, 1028, 1282, 2358
AflIII ACRYGT 1 cut(s) 2748
AhlI ACTAGT 1 cut(s) 2222
AjiI CACGTC 1 cut(s) 2749
AjnI CCWGG 1 cut(s) 2676
AjuI GAANNNNNNNTTGG 4 cut(s) 243, 275, 2813, 2845
AleI CACNNNNGTG 1 cut(s) 1275
Alw21I GWGCWC 7 cut(s) 888, 1272, 1329, 1593, 2508, 2606, 2817
Alw26I GTCTC 9 cut(s) 220, 408, 699, 1002, 1637, 1746, 1941, 2087, 2607
AlwI GGATC 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 1583
AoxI GGCC 7 cut(s) 247, 421, 1765, 1880, 2238, 2451, 2852
ApeKI GCWGC 8 cut(s) 34, 1042, 1160, 1739, 2243, 2684, 2861, 2890
ApoI RAATTY 2 cut(s) 54, 1481
ArsI GACNNNNNNTTYG 4 cut(s) 1171, 1203, 2133, 2165
AspLEI GCGC 2 cut(s) 2518, 2548
AspS9I GGNCC 6 cut(s) 582, 903, 2238, 2267, 2433, 2833
AsuC2I CCSGG 2 cut(s) 506, 1622
AsuII TTCGAA 2 cut(s) 1717, 1831
AsuNHI GCTAGC 2 cut(s) 535, 2185
AvaI CYCGRG 1 cut(s) 1583
AvaII GGWCC 5 cut(s) 582, 903, 2267, 2433, 2833
AxyI CCTNAGG 1 cut(s) 418
BaeGI GKGCMC 1 cut(s) 2659
BaeI ACNNNNGTAYC 4 cut(s) 226, 226, 259, 259
BalI TGGCCA 1 cut(s) 1882
BanI GGYRCC 1 cut(s) 2656
BanII GRGCYC 5 cut(s) 888, 1329, 1593, 2508, 2817
BauI CACGAG 2 cut(s) 521, 2507
BbsI GAAGAC 3 cut(s) 267, 1508, 1816
Bbv12I GWGCWC 7 cut(s) 888, 1272, 1329, 1593, 2508, 2606, 2817
BbvCI CCTCAGC 1 cut(s) 2807
BbvI GCAGC 8 cut(s) 46, 1054, 1147, 1726, 2230, 2671, 2873, 2877
BccI CCATC 8 cut(s) 274, 336, 469, 1373, 1462, 1540, 1694, 2134
BciT130I CCWGG 1 cut(s) 2678
BciVI GTATCC 1 cut(s) 361
BclI TGATCA 5 cut(s) 193, 1050, 1117, 1936, 2398
BcnI CCSGG 2 cut(s) 506, 1622
BcoDI GTCTC 9 cut(s) 220, 408, 699, 1002, 1637, 1746, 1941, 2087, 2607
BcuI ACTAGT 1 cut(s) 2222
BfaI CTAG 7 cut(s) 231, 536, 1151, 2147, 2186, 2223, 2229
BfmI CTRYAG 5 cut(s) 1161, 1571, 2034, 2859, 2891
BfoI RGCGCY 2 cut(s) 2519, 2549
BfuAI ACCTGC 3 cut(s) 1154, 2780, 2889
BfuI GTATCC 1 cut(s) 361
BmcAI AGTACT 3 cut(s) 979, 1502, 2227
Bme1390I CCNGG 3 cut(s) 506, 1622, 2678
Bme18I GGWCC 5 cut(s) 582, 903, 2267, 2433, 2833
BmeT110I CYCGRG 1 cut(s) 1583
BmgBI CACGTC 1 cut(s) 2749
BmgT120I GGNCC 6 cut(s) 582, 903, 2238, 2267, 2433, 2833
BmiI GGNNCC 8 cut(s) 144, 583, 904, 1103, 1303, 2239, 2627, 2658
BmrFI CCNGG 3 cut(s) 506, 1622, 2678
BmrI ACTGGG 1 cut(s) 1080
BmsI GCATC 5 cut(s) 98, 1667, 1791, 2186, 2793
BmtI GCTAGC 2 cut(s) 539, 2189
BmuI ACTGGG 1 cut(s) 1080
BpiI GAAGAC 3 cut(s) 267, 1508, 1816
BplI GAGNNNNNCTC 2 cut(s) 904, 936
BpmI CTGGAG 3 cut(s) 1288, 2738, 2801
Bpu10I CCTNAGC 2 cut(s) 2807, 2849
Bpu14I TTCGAA 2 cut(s) 1717, 1831
BpuEI CTTGAG 1 cut(s) 2504
BpuMI CCSGG 2 cut(s) 506, 1622
BsaAI YACGTR 2 cut(s) 1692, 2527
BsaI GGTCTC 1 cut(s) 220
BsaWI WCCGGW 4 cut(s) 113, 350, 584, 2710
BsaXI ACNNNNNCTCC 2 cut(s) 2711, 2741
Bsc4I CCNNNNNNNGG 8 cut(s) 164, 252, 546, 648, 912, 1028, 1282, 2358
Bse118I RCCGGY 2 cut(s) 245, 719
Bse1I ACTGG 5 cut(s) 240, 628, 936, 1086, 1746
Bse21I CCTNAGG 1 cut(s) 418
Bse3DI GCAATG 3 cut(s) 1473, 2180, 2239
BseBI CCWGG 1 cut(s) 2678
BseGI GGATG 6 cut(s) 328, 377, 1230, 1454, 1780, 1960
BseLI CCNNNNNNNGG 8 cut(s) 164, 252, 546, 648, 912, 1028, 1282, 2358
BseMI GCAATG 3 cut(s) 1473, 2180, 2239
BseMII CTCAG 6 cut(s) 432, 588, 627, 774, 1041, 2798
BseNI ACTGG 5 cut(s) 240, 628, 936, 1086, 1746
BseRI GAGGAG 6 cut(s) 384, 792, 909, 1601, 1943, 2614
BseSI GKGCMC 1 cut(s) 2659
BseXI GCAGC 8 cut(s) 46, 1054, 1147, 1726, 2230, 2671, 2873, 2877
BseYI CCCAGC 4 cut(s) 742, 1462, 2168, 2583
BsgI GTGCAG 1 cut(s) 1342
Bsh1236I CGCG 1 cut(s) 710
BshFI GGCC 7 cut(s) 249, 423, 1767, 1882, 2240, 2453, 2854
BshNI GGYRCC 1 cut(s) 2656
BsiHKAI GWGCWC 7 cut(s) 888, 1272, 1329, 1593, 2508, 2606, 2817
BsiHKCI CYCGRG 1 cut(s) 1583
BsiSI CCGG 9 cut(s) 114, 140, 246, 351, 506, 585, 720, 1621, 2711
BsiWI CGTACG 1 cut(s) 1692
BslFI GGGAC 5 cut(s) 295, 1637, 2230, 2588, 2600
BslI CCNNNNNNNGG 8 cut(s) 164, 252, 546, 648, 912, 1028, 1282, 2358
BsmAI GTCTC 9 cut(s) 220, 408, 699, 1002, 1637, 1746, 1941, 2087, 2607
BsmBI CGTCTC 3 cut(s) 408, 699, 1002
BsmFI GGGAC 5 cut(s) 295, 1637, 2230, 2588, 2600
BsmI GAATGC 1 cut(s) 1321
BsnI GGCC 7 cut(s) 249, 423, 1767, 1882, 2240, 2453, 2854
Bso31I GGTCTC 1 cut(s) 220
BsoBI CYCGRG 1 cut(s) 1583
Bsp119I TTCGAA 2 cut(s) 1717, 1831
Bsp1286I GDGCHC 8 cut(s) 888, 1272, 1329, 1593, 2508, 2606, 2659, 2817
Bsp19I CCATGG 3 cut(s) 1347, 1362, 1475
BspANI GGCC 7 cut(s) 249, 423, 1767, 1882, 2240, 2453, 2854
BspCNI CTCAG 6 cut(s) 431, 587, 626, 775, 1040, 2799
BspFNI CGCG 1 cut(s) 710
BspHI TCATGA 1 cut(s) 1053
BspLI GGNNCC 8 cut(s) 144, 583, 904, 1103, 1303, 2239, 2627, 2658
BspMAI CTGCAG 3 cut(s) 1165, 2863, 2895
BspMI ACCTGC 3 cut(s) 1154, 2780, 2889
BspOI GCTAGC 2 cut(s) 539, 2189
BspPI GGATC 1 cut(s) 704
BspT104I TTCGAA 2 cut(s) 1717, 1831
BspT107I GGYRCC 1 cut(s) 2656
BspTNI GGTCTC 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 1059
BsrDI GCAATG 3 cut(s) 1473, 2180, 2239
BsrFI RCCGGY 2 cut(s) 245, 719
BsrI ACTGG 5 cut(s) 240, 628, 936, 1086, 1746
BssAI RCCGGY 2 cut(s) 245, 719
BssSI CACGAG 2 cut(s) 521, 2507
BssT1I CCWWGG 6 cut(s) 540, 568, 1347, 1362, 1475, 2454
Bst2BI CACGAG 2 cut(s) 521, 2507
Bst2UI CCWGG 1 cut(s) 2678
Bst4CI ACNGT 9 cut(s) 103, 894, 1277, 1409, 1952, 2213, 2335, 2664, 2904
Bst6I CTCTTC 4 cut(s) 218, 471, 481, 864
BstBAI YACGTR 2 cut(s) 1692, 2527
BstBI TTCGAA 2 cut(s) 1717, 1831
BstC8I GCNNGC 6 cut(s) 537, 855, 888, 1329, 1357, 2187
BstDEI CTNAG 9 cut(s) 418, 574, 613, 783, 1027, 1388, 1733, 2807, 2849
BstDSI CCRYGG 4 cut(s) 1276, 1347, 1362, 1475
BstENI CCTNNNNNAGG 1 cut(s) 1026
BstF5I GGATG 6 cut(s) 328, 377, 1230, 1454, 1780, 1960
BstFNI CGCG 1 cut(s) 710
BstH2I RGCGCY 2 cut(s) 2519, 2549
BstHHI GCGC 2 cut(s) 2518, 2548
BstMAI GTCTC 9 cut(s) 220, 408, 699, 1002, 1637, 1746, 1941, 2087, 2607
BstMWI GCNNNNNNNGC 9 cut(s) 551, 716, 1305, 1588, 2543, 2654, 2803, 2812, 2890
BstNI CCWGG 1 cut(s) 2678
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 3 cut(s) 504, 1620, 2676
BstSFI CTRYAG 5 cut(s) 1161, 1571, 2034, 2859, 2891
BstSLI GKGCMC 1 cut(s) 2659
BstUI CGCG 1 cut(s) 710
BstV1I GCAGC 8 cut(s) 46, 1054, 1147, 1726, 2230, 2671, 2873, 2877
BstV2I GAAGAC 3 cut(s) 267, 1508, 1816
BstXI CCANNNNNNTGG 1 cut(s) 2539
Bsu36I CCTNAGG 1 cut(s) 418
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 7 cut(s) 249, 423, 1767, 1882, 2240, 2453, 2854
BtgI CCRYGG 4 cut(s) 1276, 1347, 1362, 1475
BtgZI GCGATG 1 cut(s) 1518
BtrI CACGTC 1 cut(s) 2749
BtsCI GGATG 6 cut(s) 328, 377, 1230, 1454, 1780, 1960
BtsI GCAGTG 1 cut(s) 1653
BtsIMutI CAGTG 7 cut(s) 169, 943, 1093, 1653, 2218, 2340, 2900
BveI ACCTGC 3 cut(s) 1154, 2780, 2889
Cac8I GCNNGC 6 cut(s) 537, 855, 888, 1329, 1357, 2187
CciI TCATGA 1 cut(s) 1053
CfoI GCGC 2 cut(s) 2518, 2548
Cfr10I RCCGGY 2 cut(s) 245, 719
Cfr13I GGNCC 6 cut(s) 582, 903, 2238, 2267, 2433, 2833
CseI GACGC 2 cut(s) 716, 1801
CsiI ACCWGGT 1 cut(s) 2676
CspCI CAANNNNNGTGG 2 cut(s) 2345, 2380
DdeI CTNAG 9 cut(s) 418, 574, 613, 783, 1027, 1388, 1733, 2807, 2849
EaeI YGGCCR 2 cut(s) 247, 1880
Eam1104I CTCTTC 4 cut(s) 218, 471, 481, 864
EarI CTCTTC 4 cut(s) 218, 471, 481, 864
EciI GGCGGA 1 cut(s) 2705
Ecl136II GAGCTC 5 cut(s) 886, 1327, 1591, 2506, 2815
Eco130I CCWWGG 6 cut(s) 540, 568, 1347, 1362, 1475, 2454
Eco24I GRGCYC 5 cut(s) 888, 1329, 1593, 2508, 2817
Eco31I GGTCTC 1 cut(s) 220
Eco47I GGWCC 5 cut(s) 582, 903, 2267, 2433, 2833
Eco53kI GAGCTC 5 cut(s) 886, 1327, 1591, 2506, 2815
Eco57I CTGAAG 4 cut(s) 668, 1278, 1881, 2870
Eco81I CCTNAGG 1 cut(s) 418
Eco88I CYCGRG 1 cut(s) 1583
EcoICRI GAGCTC 5 cut(s) 886, 1327, 1591, 2506, 2815
EcoNI CCTNNNNNAGG 1 cut(s) 1026
EcoO109I RGGNCCY 1 cut(s) 2433
EcoRII CCWGG 1 cut(s) 2676
EcoT14I CCWWGG 6 cut(s) 540, 568, 1347, 1362, 1475, 2454
EcoT38I GRGCYC 5 cut(s) 888, 1329, 1593, 2508, 2817
ErhI CCWWGG 6 cut(s) 540, 568, 1347, 1362, 1475, 2454
Esp3I CGTCTC 3 cut(s) 408, 699, 1002
FaqI GGGAC 5 cut(s) 295, 1637, 2230, 2588, 2600
FauNDI CATATG 1 cut(s) 2579
FbaI TGATCA 5 cut(s) 193, 1050, 1117, 1936, 2398
FblI GTMKAC 1 cut(s) 1815
FokI GGATG 6 cut(s) 315, 364, 1237, 1441, 1787, 1947
FriOI GRGCYC 5 cut(s) 888, 1329, 1593, 2508, 2817
FspBI CTAG 7 cut(s) 231, 536, 1151, 2147, 2186, 2223, 2229
GlaI GCGC 2 cut(s) 2517, 2547
GsaI CCCAGC 4 cut(s) 746, 1466, 2172, 2587
GsuI CTGGAG 3 cut(s) 1288, 2738, 2801
HaeII RGCGCY 2 cut(s) 2519, 2549
HaeIII GGCC 7 cut(s) 249, 423, 1767, 1882, 2240, 2453, 2854
HapII CCGG 9 cut(s) 114, 140, 246, 351, 506, 585, 720, 1621, 2711
HgaI GACGC 2 cut(s) 716, 1801
HhaI GCGC 2 cut(s) 2518, 2548
Hin6I GCGC 2 cut(s) 2516, 2546
HinP1I GCGC 2 cut(s) 2516, 2546
HincII GTYRAC 5 cut(s) 629, 1405, 1424, 1816, 2322
HindII GTYRAC 5 cut(s) 629, 1405, 1424, 1816, 2322
HindIII AAGCTT 4 cut(s) 257, 839, 1368, 1863
HpaII CCGG 9 cut(s) 114, 140, 246, 351, 506, 585, 720, 1621, 2711
Hpy166II GTNNAC 7 cut(s) 629, 1405, 1424, 1618, 1816, 2322, 2746
Hpy188III TCNNGA 8 cut(s) 597, 906, 1054, 1288, 1312, 1487, 1900, 2818
Hpy8I GTNNAC 7 cut(s) 629, 1405, 1424, 1618, 1816, 2322, 2746
Hpy99I CGWCG 5 cut(s) 1016, 1209, 1817, 2504, 2645
HpyAV CCTTC 6 cut(s) 437, 1008, 1189, 1256, 1768, 1974
HpyCH4III ACNGT 9 cut(s) 103, 894, 1277, 1409, 1952, 2213, 2335, 2664, 2904
HpyCH4IV ACGT 4 cut(s) 1614, 1691, 2526, 2748
HpyF10VI GCNNNNNNNGC 9 cut(s) 551, 716, 1305, 1588, 2543, 2654, 2803, 2812, 2890
HpyF3I CTNAG 9 cut(s) 418, 574, 613, 783, 1027, 1388, 1733, 2807, 2849
HpySE526I ACGT 4 cut(s) 1614, 1691, 2526, 2748
HspAI GCGC 2 cut(s) 2516, 2546
Ksp22I TGATCA 5 cut(s) 193, 1050, 1117, 1936, 2398
Lsp1109I GCAGC 8 cut(s) 46, 1054, 1147, 1726, 2230, 2671, 2873, 2877
LweI GCATC 5 cut(s) 98, 1667, 1791, 2186, 2793
MabI ACCWGGT 1 cut(s) 2676
MaeI CTAG 7 cut(s) 231, 536, 1151, 2147, 2186, 2223, 2229
MaeII ACGT 4 cut(s) 1614, 1691, 2526, 2748
MbiI CCGCTC 1 cut(s) 1059
MhlI GDGCHC 8 cut(s) 888, 1272, 1329, 1593, 2508, 2606, 2659, 2817
MlsI TGGCCA 1 cut(s) 1882
MluCI AATT 8 cut(s) 54, 203, 479, 795, 1209, 1481, 2085, 2098
MluNI TGGCCA 1 cut(s) 1882
MlyI GAGTC 5 cut(s) 286, 910, 946, 1748, 2130
MmeI TCCRAC 1 cut(s) 2376
Mox20I TGGCCA 1 cut(s) 1882
MscI TGGCCA 1 cut(s) 1882
MseI TTAA 3 cut(s) 98, 132, 2063
MslI CAYNNNNRTG 2 cut(s) 1275, 2918
Msp20I TGGCCA 1 cut(s) 1882
MspA1I CMGCKG 3 cut(s) 1045, 2243, 2890
MspI CCGG 9 cut(s) 114, 140, 246, 351, 506, 585, 720, 1621, 2711
MspR9I CCNGG 3 cut(s) 506, 1622, 2678
Mva1269I GAATGC 1 cut(s) 1321
MvaI CCWGG 1 cut(s) 2678
MvnI CGCG 1 cut(s) 710
MwoI GCNNNNNNNGC 9 cut(s) 551, 716, 1305, 1588, 2543, 2654, 2803, 2812, 2890
NciI CCSGG 2 cut(s) 506, 1622
NcoI CCATGG 3 cut(s) 1347, 1362, 1475
NdeI CATATG 1 cut(s) 2579
NheI GCTAGC 2 cut(s) 535, 2185
NlaIV GGNNCC 8 cut(s) 144, 583, 904, 1103, 1303, 2239, 2627, 2658
NmeAIII GCCGAG 1 cut(s) 700
NmuCI GTSAC 9 cut(s) 109, 967, 1888, 1952, 2047, 2335, 2527, 2898, 2911
NspI RCATGY 1 cut(s) 742
NspV TTCGAA 2 cut(s) 1717, 1831
OliI CACNNNNGTG 1 cut(s) 1275
PagI TCATGA 1 cut(s) 1053
PaqCI CACCTGC 3 cut(s) 1154, 2780, 2889
PctI GAATGC 1 cut(s) 1321
PfeI GAWTC 7 cut(s) 217, 1238, 1291, 1343, 1667, 2668, 2821
Pfl23II CGTACG 1 cut(s) 1692
PflFI GACNNNGTC 1 cut(s) 1820
PflMI CCANNNNNTGG 2 cut(s) 164, 546
PleI GAGTC 5 cut(s) 286, 910, 946, 1748, 2130
PpsI GAGTC 5 cut(s) 286, 910, 946, 1748, 2130
Ppu21I YACGTR 2 cut(s) 1692, 2527
PpuMI RGGWCCY 1 cut(s) 2433
Psp124BI GAGCTC 5 cut(s) 888, 1329, 1593, 2508, 2817
Psp5II RGGWCCY 1 cut(s) 2433
Psp6I CCWGG 1 cut(s) 2676
PspFI CCCAGC 4 cut(s) 742, 1462, 2168, 2583
PspGI CCWGG 1 cut(s) 2676
PspLI CGTACG 1 cut(s) 1692
PspN4I GGNNCC 8 cut(s) 144, 583, 904, 1103, 1303, 2239, 2627, 2658
PspPI GGNCC 6 cut(s) 582, 903, 2238, 2267, 2433, 2833
PspPPI RGGWCCY 1 cut(s) 2433
PstI CTGCAG 3 cut(s) 1165, 2863, 2895
PsyI GACNNNGTC 1 cut(s) 1820
PvuII CAGCTG 2 cut(s) 1045, 2890
RseI CAYNNNNRTG 2 cut(s) 1275, 2918
SacI GAGCTC 5 cut(s) 888, 1329, 1593, 2508, 2817
SalI GTCGAC 1 cut(s) 1814
SaqAI TTAA 3 cut(s) 98, 132, 2063
Sau96I GGNCC 6 cut(s) 582, 903, 2238, 2267, 2433, 2833
ScaI AGTACT 3 cut(s) 979, 1502, 2227
SchI GAGTC 5 cut(s) 286, 910, 946, 1748, 2130
ScrFI CCNGG 3 cut(s) 506, 1622, 2678
SduI GDGCHC 8 cut(s) 888, 1272, 1329, 1593, 2508, 2606, 2659, 2817
SexAI ACCWGGT 1 cut(s) 2676
SfaNI GCATC 5 cut(s) 98, 1667, 1791, 2186, 2793
SfcI CTRYAG 5 cut(s) 1161, 1571, 2034, 2859, 2891
SfuI TTCGAA 2 cut(s) 1717, 1831
SinI GGWCC 5 cut(s) 582, 903, 2267, 2433, 2833
SmiMI CAYNNNNRTG 2 cut(s) 1275, 2918
SmlI CTYRAG 1 cut(s) 2519
SmoI CTYRAG 1 cut(s) 2519
SpeI ACTAGT 1 cut(s) 2222
Sse9I AATT 8 cut(s) 54, 203, 479, 795, 1209, 1481, 2085, 2098
SspI AATATT 1 cut(s) 2311
SspMI CTAG 7 cut(s) 231, 536, 1151, 2147, 2186, 2223, 2229
SstI GAGCTC 5 cut(s) 888, 1329, 1593, 2508, 2817
StyD4I CCNGG 3 cut(s) 504, 1620, 2676
StyI CCWWGG 6 cut(s) 540, 568, 1347, 1362, 1475, 2454
TaaI ACNGT 9 cut(s) 103, 894, 1277, 1409, 1952, 2213, 2335, 2664, 2904
TaiI ACGT 4 cut(s) 1617, 1694, 2529, 2751
TaqI TCGA 8 cut(s) 1178, 1207, 1519, 1717, 1815, 1831, 2083, 2640
TaqII GACCGA 1 cut(s) 2255
TasI AATT 8 cut(s) 54, 203, 479, 795, 1209, 1481, 2085, 2098
TatI WGTACW 4 cut(s) 977, 1500, 2225, 2421
TauI GCSGC 4 cut(s) 991, 1302, 2243, 2690
TfiI GAWTC 7 cut(s) 217, 1238, 1291, 1343, 1667, 2668, 2821
Tru1I TTAA 3 cut(s) 98, 132, 2063
Tru9I TTAA 3 cut(s) 98, 132, 2063
TscAI CASTG 7 cut(s) 169, 943, 1093, 1660, 2218, 2340, 2907
TseFI GTSAC 9 cut(s) 109, 967, 1888, 1952, 2047, 2335, 2527, 2898, 2911
TseI GCWGC 8 cut(s) 34, 1042, 1160, 1739, 2243, 2684, 2861, 2890
Tsp45I GTSAC 9 cut(s) 109, 967, 1888, 1952, 2047, 2335, 2527, 2898, 2911
TspDTI ATGAA 9 cut(s) 171, 579, 882, 1164, 1173, 1750, 1816, 1949, 2554
TspGWI ACGGA 6 cut(s) 261, 617, 964, 1231, 2621, 2661
TspRI CASTG 7 cut(s) 169, 943, 1093, 1660, 2218, 2340, 2907
Tth111I GACNNNGTC 1 cut(s) 1820
Van91I CCANNNNNTGG 2 cut(s) 164, 546
VpaK11BI GGWCC 5 cut(s) 582, 903, 2267, 2433, 2833
XagI CCTNNNNNAGG 1 cut(s) 1026
XapI RAATTY 2 cut(s) 54, 1481
XceI RCATGY 1 cut(s) 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 2539
XmiI GTMKAC 1 cut(s) 1815
XspI CTAG 7 cut(s) 231, 536, 1151, 2147, 2186, 2223, 2229
ZrmI AGTACT 3 cut(s) 979, 1502, 2227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.