Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0021194

Physical Location & Seq

Genomic Coordinates Forward (+)

3700105 .. 3702594

Gene Structure 2490 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1020000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGTTTCTGGATGCTCAAAGCTTGAAAGTTTCCAGAAAGTTTTGGAGGTTAGGAAGAAGCCTATCTGAGCTTTGTTTGGGTGGCACTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCATCAGTCTTGTTTGGTTGAGGCTGAATAATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTAAATACCTTGATGTTTCTGGATGCTCAAAGATTGAGAAGTTTCCAGAAAGTTTGGAGGTTAGGAAGGAGCTATCTGATCTTTGTTTGGGTGGCACTGCAATTAAGGCGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAAGTTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCATCATTTCTCACATGAAATCTCTTGGATACCTTGATGTTTCTGGATGCTCAAAGATTGAGAAGTTTCCAGAAATTTTGGAGGTTATGAAGGAGCTATCTATTCTTTGTTTGGGTGGCACTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGATACTTTGAAACTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCATCATTTCTCACATGAAATCTCTTAAATACCTTGATGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTTGGAGGTTATGAAGAAGCTATCTGAGCTTTGTTTGGGTGGCACTGCAATTAAGAAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGGTAGGTTGAGGCTGAATAATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTAAATACCTTGATGTTTCTGGATGCTCAAAGCTTGAAAAGTTTCCAGAAATTTTGGAGGTTAGGAAGAAGCTATCTGAGCTTTGTTTGGGTGGCACTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCATCAGTCTTGTTAGGTTGAGGCTGAATAATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTAAATACCTTGATGTTTCTGGATGCTCAAAGATTGAGAAGTTTCCAGAAATTTTGGAGGTTAGGAAGGAGCTATCTGATCTTTGTTTGGGTGGCACTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAATTTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTGAGTACCTTGATGTTTCTGGATGCTCAAAGATTGAGAAGTTTCCAGAAATTTTGGAGGTTAGGAACGTGCTATCTAAGCTTTGTTTGGGTGGCACTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAATTTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCTGCATTTCTCACATGAAATCTCTTGAATACCTTGATGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAAATTTGGAGGTTATGAATGAGCTATCTGAGCTTTGTTTGGGTGGCACTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAAGTTGAATGATTGCGGAGAACTTGAGAGTCTTCCAAGCATCATTTCTCACATGAAATCTCTTAAATACCTTGATGTTTCTGGATGCTCAAAGCTTGAGAAGTTTCCAGAAATTTTGGAGGTTAGGAAGGAGCTATCTAAGCTTTGTTTGGGTGGCACTGCAATTAAGAAGCTGCCTGCTTCTATTTTAAATCTCACCAAAATTTTGGAGGTTATGAAGAAGCTATCTGAGCTTTGTTTGGGTGGCACTGCAATTAAGAAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGGTAGGTTGAGGCTGAATAATTGCAGAGAACTTGAGAGTCTTCCAAGCATCATTTCTCAGATGAAATCTCTTGAATACCTTGATGTTTCTGGATGCTCAAATCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAAGGAGCTATCTATTCTTTGTTTGGGTGGCACTGCAATTAAGGAGCTGCCTGCTTCTATTTTAAATCTCACCAGTCTTGTTACTTTGAATTTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCTCACATGAAATCTCTTGAGTACCTTGATGTTTCTGGATGCTCAAAGATTGAGAAGTTTCCCGAAAATTTGGAGGTTATGAAGAAGTTTCTCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009626 GO:0009628 GO:0009639 GO:0009870 GO:0009987 GO:0010114 GO:0012501 GO:0012505 GO:0016020 GO:0023052 GO:0031347 GO:0031349 GO:0033554 GO:0034050 GO:0042742 GO:0042802 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0080134 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

764

Amino Acids

83.99

Weight (kDa)

7.03

Isoelectric Point (pI)

41.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	21 - 94	4.1e-07	Leucine-rich repeat region
LRR_13	PF23286	65 - 133	1.2e-07	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	95 - 166	2.6e-07	Leucine-rich repeat region
LRR_13	PF23286	137 - 204	7.1e-09	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	163 - 238	3.6e-09	Leucine-rich repeat region
LRR_13	PF23286	207 - 276	3.3e-10	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	237 - 308	2.5e-06	Leucine-rich repeat region
LRR_13	PF23286	278 - 347	5.9e-10	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	281 - 370	2.2e-08	Leucine-rich repeat region
LRR_13	PF23286	349 - 417	3.3e-10	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	379 - 434	1.7e-07	Leucine-rich repeat region
LRR_13	PF23286	420 - 488	3.2e-11	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	450 - 523	8.6e-10	Leucine-rich repeat region
LRR_13	PF23286	491 - 559	6.5e-10	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	521 - 585	5.7e-07	Leucine-rich repeat region
LRR_RPS2	PF23247	529 - 674	1.9e-07	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14	PF23598	592 - 685	2.3e-08	Leucine-rich repeat region
LRR_13	PF23286	664 - 749	1.2e-13	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	684 - 758	3.6e-09	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

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Orthologous Genes (Group: OG0021194)

Species	Orthologous Gene IDs
malus_domestica	MD00G1020000.v1.1 MD00G1021300.v1.1 MD17G1208800.v1.1
pyrus_communis	pycom07g23420 pycom17g21280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	1647
AfaI	GTAC	2 cut(s)	1274, 2216
Asp700I	GAANNNNTTC	1 cut(s)	882
AspLEI	GCGC	1 cut(s)	313
AsuHPI	GGTGA	9 cut(s)	328, 541, 754, 1180, 1393, 1606, 1819, 1909, 2122
BciVI	GTATCC	1 cut(s)	413
BfoI	RGCGCY	1 cut(s)	314
BfuI	GTATCC	1 cut(s)	413
Bse1I	ACTGG	8 cut(s)	339, 552, 765, 1191, 1404, 1617, 1920, 2133
BseMII	CTCAG	6 cut(s)	57, 696, 909, 1548, 1851, 1997
BseNI	ACTGG	8 cut(s)	339, 552, 765, 1191, 1404, 1617, 1920, 2133
Bsp143I	GATC	2 cut(s)	280, 1132
BspACI	CCGC	1 cut(s)	1647
BspCNI	CTCAG	6 cut(s)	58, 697, 910, 1549, 1852, 1996
BsrI	ACTGG	8 cut(s)	339, 552, 765, 1191, 1404, 1617, 1920, 2133
BssMI	GATC	2 cut(s)	280, 1132
BstDEI	CTNAG	8 cut(s)	66, 705, 918, 1344, 1557, 1770, 1860, 1983
BstH2I	RGCGCY	1 cut(s)	314
BstHHI	GCGC	1 cut(s)	313
BstKTI	GATC	2 cut(s)	283, 1135
BstMBI	GATC	2 cut(s)	280, 1132
BstMWI	GCNNNNNNNGC	8 cut(s)	308, 317, 706, 919, 1345, 1558, 1771, 1861
BstXI	CCANNNNNNTGG	1 cut(s)	1837
BsuI	GTATCC	1 cut(s)	413
CfoI	GCGC	1 cut(s)	313
Csp6I	GTAC	2 cut(s)	1273, 2215
CviQI	GTAC	2 cut(s)	1273, 2215
DdeI	CTNAG	8 cut(s)	66, 705, 918, 1344, 1557, 1770, 1860, 1983
DpnI	GATC	2 cut(s)	282, 1134
DpnII	GATC	2 cut(s)	280, 1132
GlaI	GCGC	1 cut(s)	312
HaeII	RGCGCY	1 cut(s)	314
HhaI	GCGC	1 cut(s)	313
Hin6I	GCGC	1 cut(s)	311
HinP1I	GCGC	1 cut(s)	311
HindIII	AAGCTT	7 cut(s)	19, 659, 872, 1346, 1511, 1724, 1772
HphI	GGTGA	9 cut(s)	328, 541, 754, 1180, 1393, 1606, 1819, 1909, 2122
Hpy188I	TCNGA	9 cut(s)	67, 280, 706, 919, 1132, 1558, 1861, 1986, 2053
HpyAV	CCTTC	5 cut(s)	262, 475, 1114, 1753, 2056
HpyCH4IV	ACGT	1 cut(s)	1335
HpyF10VI	GCNNNNNNNGC	8 cut(s)	308, 317, 706, 919, 1345, 1558, 1771, 1861
HpyF3I	CTNAG	8 cut(s)	66, 705, 918, 1344, 1557, 1770, 1860, 1983
HpySE526I	ACGT	1 cut(s)	1335
HspAI	GCGC	1 cut(s)	311
Kzo9I	GATC	2 cut(s)	280, 1132
MaeII	ACGT	1 cut(s)	1335
MaeIII	GTNAC	5 cut(s)	346, 1198, 1411, 1624, 2140
MalI	GATC	2 cut(s)	282, 1134
MboI	GATC	2 cut(s)	280, 1132
MroXI	GAANNNNTTC	1 cut(s)	882
MwoI	GCNNNNNNNGC	8 cut(s)	308, 317, 706, 919, 1345, 1558, 1771, 1861
NdeII	GATC	2 cut(s)	280, 1132
PdmI	GAANNNNTTC	1 cut(s)	882
RsaI	GTAC	2 cut(s)	1274, 2216
RsaNI	GTAC	2 cut(s)	1273, 2215
Sau3AI	GATC	2 cut(s)	280, 1132
SsiI	CCGC	1 cut(s)	1647
TaiI	ACGT	1 cut(s)	1338
XmnI	GAANNNNTTC	1 cut(s)	882

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.