MD17G1208800.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0021194
Physical Location & Seq
Forward (+)
25165196 .. 25166091
896 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1208800.v1.1.491

Sequence Viewer

Length: 270 bp
ATGAATATGCTATCTGAGCTTTATTTGGGTGGCACTGCAATTAAGGAACTGCCCGCGTCAGTTTTAAATCTCACTAGTCTTGTTACTTTGATGCTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATATGTCACATGAAATCTCTTCGATACCTTGATGTTTCTGGATGCTCAAATCTTGAGAAGTTTCCGGAAAATTCAGAGGTCATGAATAAGATATCTACAGAGAACTTGAGAGTTTTCCAAGCAGCATTTCTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

90

Amino Acids

9.92

Weight (kDa)

4.97

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 1 - 64 2.2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_8 PF13855 4 - 59 9.3e-06 Leucine rich repeat
LRR_14 PF23598 7 - 77 1.5e-09 Leucine-rich repeat region
LRR_Zer-1 PF25013 21 - 77 2.9e-08 Zer-1-like, Leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021194)

Species Orthologous Gene IDs
malus_domestica MD00G1020000.v1.1 MD00G1021300.v1.1 MD17G1208800.v1.1
pyrus_communis pycom07g23420 pycom17g21280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 56
AccIII TCCGGA 1 cut(s) 196
AciI CCGC 1 cut(s) 54
AcsI RAATTY 1 cut(s) 202
AhlI ACTAGT 1 cut(s) 74
AjuI GAANNNNNNNTTGG 1 cut(s) 243
AluBI AGCT 1 cut(s) 19
AluI AGCT 1 cut(s) 19
Aor13HI TCCGGA 1 cut(s) 196
ApeKI GCWGC 2 cut(s) 129, 254
ApoI RAATTY 1 cut(s) 202
BbsI GAAGAC 1 cut(s) 113
BbvI GCAGC 2 cut(s) 141, 266
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 228
BisI GCNGC 2 cut(s) 130, 255
BlsI GCNGC 2 cut(s) 131, 256
BmsI GCATC 2 cut(s) 81, 164
BpiI GAAGAC 1 cut(s) 113
BpuEI CTTGAG 3 cut(s) 134, 206, 259
BsaWI WCCGGW 1 cut(s) 196
BseAI TCCGGA 1 cut(s) 196
BseGI GGATG 1 cut(s) 179
BseMII CTCAG 1 cut(s) 6
BseXI GCAGC 2 cut(s) 141, 266
Bsh1236I CGCG 1 cut(s) 56
BsiSI CCGG 1 cut(s) 197
Bsp13I TCCGGA 1 cut(s) 196
BspACI CCGC 1 cut(s) 54
BspCNI CTCAG 1 cut(s) 7
BspEI TCCGGA 1 cut(s) 196
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 1 cut(s) 213
Bst6I CTCTTC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 1 cut(s) 15
BstF5I GGATG 1 cut(s) 179
BstFNI CGCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 1 cut(s) 16
BstSFI CTRYAG 1 cut(s) 228
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 2 cut(s) 141, 266
BstV2I GAAGAC 1 cut(s) 113
BtsCI GGATG 1 cut(s) 179
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 54
CciI TCATGA 1 cut(s) 213
CseI GACGC 1 cut(s) 45
CviAII CATG 3 cut(s) 142, 214, 267
CviJI RGCY 1 cut(s) 19
CviKI_1 RGCY 1 cut(s) 19
DdeI CTNAG 1 cut(s) 15
DraI TTTAAA 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco32I GATATC 1 cut(s) 225
EcoRV GATATC 1 cut(s) 225
FaeI CATG 3 cut(s) 145, 217, 270
FaiI YATR 6 cut(s) 8, 134, 136, 143, 215, 268
FatI CATG 3 cut(s) 141, 213, 266
FauI CCCGC 1 cut(s) 61
FauNDI CATATG 1 cut(s) 134
Fnu4HI GCNGC 2 cut(s) 130, 255
FokI GGATG 1 cut(s) 186
Fsp4HI GCNGC 2 cut(s) 130, 255
FspBI CTAG 1 cut(s) 75
GluI GCNGC 2 cut(s) 130, 255
HapII CCGG 1 cut(s) 197
HgaI GACGC 1 cut(s) 45
Hin1II CATG 3 cut(s) 145, 217, 270
HinfI GANTC 1 cut(s) 118
HpaII CCGG 1 cut(s) 197
Hpy188I TCNGA 2 cut(s) 16, 208
Hpy188III TCNNGA 4 cut(s) 171, 185, 197, 214
HpyCH4V TGCA 2 cut(s) 38, 105
HpyF10VI GCNNNNNNNGC 1 cut(s) 16
HpyF3I CTNAG 1 cut(s) 15
Hsp92II CATG 3 cut(s) 145, 217, 270
Kpn2I TCCGGA 1 cut(s) 196
LpnPI CCDG 2 cut(s) 156, 210
Lsp1109I GCAGC 2 cut(s) 141, 266
LweI GCATC 2 cut(s) 81, 164
MaeI CTAG 1 cut(s) 75
MaeIII GTNAC 2 cut(s) 82, 137
MboII GAAGA 2 cut(s) 113, 143
MluCI AATT 2 cut(s) 39, 202
MlyI GAGTC 1 cut(s) 127
MnlI CCTC 1 cut(s) 202
MroI TCCGGA 1 cut(s) 196
MseI TTAA 2 cut(s) 42, 65
MspI CCGG 1 cut(s) 197
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 1 cut(s) 16
NdeI CATATG 1 cut(s) 134
NlaIII CATG 3 cut(s) 145, 217, 270
NmuCI GTSAC 1 cut(s) 137
PagI TCATGA 1 cut(s) 213
PkrI GCNGC 2 cut(s) 131, 256
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
SaqAI TTAA 2 cut(s) 42, 65
SatI GCNGC 2 cut(s) 130, 255
SchI GAGTC 1 cut(s) 127
SetI ASST 3 cut(s) 21, 162, 213
SfaNI GCATC 2 cut(s) 81, 164
SfcI CTRYAG 1 cut(s) 228
SmlI CTYRAG 3 cut(s) 113, 185, 238
SmoI CTYRAG 3 cut(s) 113, 185, 238
SpeI ACTAGT 1 cut(s) 74
Sse9I AATT 2 cut(s) 39, 202
SsiI CCGC 1 cut(s) 54
SspMI CTAG 1 cut(s) 75
TaqI TCGA 1 cut(s) 154
TasI AATT 2 cut(s) 39, 202
Tru1I TTAA 2 cut(s) 42, 65
Tru9I TTAA 2 cut(s) 42, 65
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 2 cut(s) 129, 254
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 3 cut(s) 17, 158, 230
TspRI CASTG 1 cut(s) 40
XapI RAATTY 1 cut(s) 202
XspI CTAG 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.