MD00G1032500.v1.1

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015991
Physical Location & Seq
Reverse (-)
5679204 .. 5683207
4004 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1032500.v1.1.491

Sequence Viewer

Length: 1560 bp
ATGAACAAGAAGAAGTCAACTTCCCAACTTACCCTTTTCGTCTTCCTCTCATTCTCTCTCACTTTCTCCTTTTCTTCTTCCCCAACACCCAGAAAGCCGCCTCCTCTTCCCATTTTGCCCCTCCCCTCTTCCCCGCAGCTCCAATGGCAGCTCGGCCACATGGCAATGTTCCTCCACTTCGGACCCAACACCTTCACGGATTCCGAGTGGGGCACCGGCCACGTCGACCCCTCGGTCTTCAACCCCGCCAAGCTCAACGCCGCTCAGTGGGTCCGAGTCGCCAAAGACTCGGGCTTTTCGCGCGTAATTCTCACCGCCAAACACCACGACGGGTTCTGCTTGTGGCCAACTGAGTACACCAACTACTCCGTAAGCTCCAGCCCATGGAGGAGTGGCGGCGGTGACGTCGTTGGTGAGCTGGCGGCGGCGGCTAGGGACGCGGGTATTGCATTGGGACTTTACCTTTCGCCGTGGGATCGGCACGAACCGTGCTATGGGAAGACTTTGGAGTACAATGAGTTCTATATGGGTCAAATGACAGAATTGCTCACAAGGTATGGAGAGATTAAGGAGGTTTGGTTGGATGGTGCAAAGGGGGAAGGGGAGAAGGACATGGAATATTTCTTTGATTCTTGGTTCAGTCTCATTCACCAACACCAGCCCGGGGCTGTCATTTTTTCTGATGCCGGTCCTGACACCAGGTGGGTTGGGGATGAAGCTGGCGTTGCTGGGTCTACTTGTTGGTCCCTTTTCAATCGGAGTGATGCCAAGATTGGCGACACTGATTACCAATACTCAAATGGAGGAGATCCCTTTGGGCATGATTGGGTACCAGCTGAGTGCGATGTTTCAATCAGGCCTGGTTGGTTTTGGCATTCGTTGGAAGTTCCAAAATCCGCCAGGACCCTTCTTGACCTATACTACAAATCGATTGGAAGAAACTGTCTCTTGTTACTCAATGTACCTCCAAACTCTTCAGGTCTCATATCTGCAGAAGATATTCAAGTGCTTCAAGAATTCAATGACCTCCGGAAGTCGATATTCTCATATAATCTGGCCAAAACGGCCCTTATCAACGCGAGCAGTACACGAGGAGGCAGTAACAATTCTTGGTTCACCCCCAGTAATGTCGTTCAAGAAGGTATTGATTCATATTGGGCTCCTGAGGGAAACCAGTCGAAATGGGTTCTATCCTTGAACCTTCAGAAATTAGTGTCATTCAACATTCTGCAAGTTCAAGAGCCGATTCAAATGGGACAGCGAGTTATCGAGTTCCATCTTGAGGTCCTGAATGAGGAGAGGAAGTGGAATAAAGTCATCAACGGAACCACAATCGGATATCAGAGGCTTCTGCAGTTTCCAACCGTAAAATCTCAGTACTTGAGGTTTGTTATTGACAAGTCCCGGGCAGACCCGCTTATCTCGTACCTGGGTTTATATATCGATCGATATTCGATTTTGAGCAACTTATCTCATACAACCTCACACGCAAGCAGAAATCGCAGTCAAATTCTTAATTACCATATCGAATCCAACCATTCTCAGGTTGCTGCAATATAG

Protein Analysis

520

Amino Acids

58.42

Weight (kDa)

6.11

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha_L_fucos PF01120 78 - 340 2.6e-34 Alpha-L-fucosidase
F5_F8_type_C PF00754 358 - 467 5.5e-07 F5/8 type C domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015991)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28100
fragaria_vesca FvH4_3g30940
malus_domestica MD00G1032500.v1.1
prunus_persica Prupe.6G109300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0057471
rosa_laevigata RLG00000035158
rosa_multiflora Rmu_sc0000508.1_g000012 Rmu_sc0003730.1_g000020
rosa_roxburghii Rroxscaffold_1G00022980
rosa_rugosa Rorug05G0307500
rosa_samantha Rh5AG376100 Rh5BG387000 Rh5CG410400 Rh5DG401200
rosa_wichuraiana Rw5G035510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AatII GACGTC 1 cut(s) 408
Acc65I GGTACC 1 cut(s) 829
AccB1I GGYRCC 2 cut(s) 212, 829
AccB7I CCANNNNNTGG 1 cut(s) 384
AccBSI CCGCTC 1 cut(s) 263
AccI GTMKAC 2 cut(s) 225, 734
AccII CGCG 4 cut(s) 301, 303, 440, 1079
AccIII TCCGGA 1 cut(s) 1029
AclWI GGATC 2 cut(s) 483, 803
AcoI YGGCCR 4 cut(s) 154, 217, 344, 1056
AcsI RAATTY 2 cut(s) 1016, 1509
AcuI CTGAAG 2 cut(s) 960, 1187
AcyI GRCGYC 1 cut(s) 405
AdeI CACNNNGTG 1 cut(s) 702
AfaI GTAC 7 cut(s) 356, 512, 831, 963, 1087, 1379, 1427
AfiI CCNNNNNNNGG 7 cut(s) 267, 384, 494, 664, 1282, 1294, 1543
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 4 cut(s) 698, 859, 899, 1428
AluBI AGCT 7 cut(s) 139, 151, 253, 375, 418, 719, 836
AluI AGCT 7 cut(s) 139, 151, 253, 375, 418, 719, 836
Alw26I GTCTC 3 cut(s) 647, 950, 986
AlwI GGATC 2 cut(s) 483, 803
Ama87I CYCGRG 3 cut(s) 289, 662, 1404
Aor13HI TCCGGA 1 cut(s) 1029
AoxI GGCC 6 cut(s) 154, 217, 344, 857, 1056, 1065
ApeKI GCWGC 3 cut(s) 136, 148, 1550
ApoI RAATTY 2 cut(s) 1016, 1509
Asp700I GAANNNNTTC 1 cut(s) 999
Asp718I GGTACC 1 cut(s) 829
AspLEI GCGC 1 cut(s) 303
AspS9I GGNCC 7 cut(s) 182, 271, 689, 744, 903, 1066, 1285
AsuC2I CCSGG 4 cut(s) 663, 664, 1405, 1406
AsuHPI GGTGA 5 cut(s) 304, 413, 425, 641, 1108
AvaI CYCGRG 3 cut(s) 289, 662, 1404
AvaII GGWCC 6 cut(s) 182, 271, 689, 744, 903, 1285
AxyI CCTNAGG 1 cut(s) 1164
BaeGI GKGCMC 1 cut(s) 215
BalI TGGCCA 2 cut(s) 346, 1058
BanI GGYRCC 2 cut(s) 212, 829
BanII GRGCYC 1 cut(s) 1162
BauI CACGAG 1 cut(s) 1089
BbsI GAAGAC 3 cut(s) 34, 229, 506
BbvI GCAGC 3 cut(s) 148, 160, 1537
BccI CCATC 2 cut(s) 578, 1284
BceAI ACGGC 2 cut(s) 454, 1080
BciT130I CCWGG 4 cut(s) 700, 861, 901, 1430
BcnI CCSGG 4 cut(s) 663, 664, 1405, 1406
BcoDI GTCTC 3 cut(s) 647, 950, 986
BfaI CTAG 1 cut(s) 432
BfmI CTRYAG 2 cut(s) 990, 1352
BglI GCCNNNNNGGC 1 cut(s) 1064
BisI GCNGC 9 cut(s) 98, 137, 149, 261, 397, 423, 426, 429, 1551
BlsI GCNGC 9 cut(s) 99, 138, 150, 262, 398, 424, 427, 430, 1552
BmcAI AGTACT 1 cut(s) 1379
Bme1390I CCNGG 8 cut(s) 663, 664, 700, 861, 901, 1405, 1406, 1430
Bme18I GGWCC 6 cut(s) 182, 271, 689, 744, 903, 1285
BmeT110I CYCGRG 3 cut(s) 289, 662, 1404
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 7 cut(s) 182, 271, 689, 744, 903, 1066, 1285
BmiI GGNNCC 8 cut(s) 184, 214, 272, 746, 831, 905, 1161, 1327
BmrFI CCNGG 8 cut(s) 663, 664, 700, 861, 901, 1405, 1406, 1430
BmrI ACTGGG 1 cut(s) 1116
BmsI GCATC 2 cut(s) 673, 754
BmuI ACTGGG 1 cut(s) 1116
BpiI GAAGAC 3 cut(s) 34, 229, 506
BpmI CTGGAG 1 cut(s) 361
BpuEI CTTGAG 2 cut(s) 1301, 1402
BpuMI CCSGG 4 cut(s) 663, 664, 1405, 1406
Bsa29I ATCGAT 3 cut(s) 929, 1443, 1447
BsaHI GRCGYC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 986
BsaJI CCNNGG 7 cut(s) 231, 383, 470, 662, 663, 1404, 1429
BsaWI WCCGGW 1 cut(s) 1029
BsaXI ACNNNNNCTCC 4 cut(s) 563, 593, 1289, 1319
Bsc4I CCNNNNNNNGG 7 cut(s) 267, 384, 494, 664, 1282, 1294, 1543
Bse118I RCCGGY 2 cut(s) 215, 686
Bse1I ACTGG 2 cut(s) 1122, 1174
Bse21I CCTNAGG 1 cut(s) 1164
Bse3DI GCAATG 1 cut(s) 171
BseAI TCCGGA 1 cut(s) 1029
BseBI CCWGG 4 cut(s) 700, 861, 901, 1430
BseCI ATCGAT 3 cut(s) 929, 1443, 1447
BseDI CCNNGG 7 cut(s) 231, 383, 470, 662, 663, 1404, 1429
BseGI GGATG 2 cut(s) 589, 718
BseLI CCNNNNNNNGG 7 cut(s) 267, 384, 494, 664, 1282, 1294, 1543
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 6 cut(s) 278, 342, 828, 1155, 1388, 1556
BseNI ACTGG 2 cut(s) 1122, 1174
BseRI GAGGAG 5 cut(s) 93, 403, 819, 1107, 1310
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 3 cut(s) 148, 160, 1537
BseYI CCCAGC 1 cut(s) 728
Bsh1236I CGCG 4 cut(s) 301, 303, 440, 1079
Bsh1285I CGRYCG 1 cut(s) 1447
BshFI GGCC 6 cut(s) 156, 219, 346, 859, 1058, 1067
BshNI GGYRCC 2 cut(s) 212, 829
BshVI ATCGAT 3 cut(s) 929, 1443, 1447
BsiEI CGRYCG 1 cut(s) 1447
BsiHKCI CYCGRG 3 cut(s) 289, 662, 1404
BsiSI CCGG 5 cut(s) 216, 663, 687, 1030, 1405
BslFI GGGAC 5 cut(s) 449, 468, 730, 1269, 1387
BslI CCNNNNNNNGG 7 cut(s) 267, 384, 494, 664, 1282, 1294, 1543
BsmAI GTCTC 3 cut(s) 647, 950, 986
BsmFI GGGAC 5 cut(s) 449, 468, 730, 1269, 1387
BsmI GAATGC 1 cut(s) 874
BsnI GGCC 6 cut(s) 156, 219, 346, 859, 1058, 1067
Bso31I GGTCTC 1 cut(s) 986
BsoBI CYCGRG 3 cut(s) 289, 662, 1404
Bsp1286I GDGCHC 2 cut(s) 215, 1162
Bsp13I TCCGGA 1 cut(s) 1029
Bsp143I GATC 3 cut(s) 475, 808, 1444
Bsp19I CCATGG 1 cut(s) 383
BspANI GGCC 6 cut(s) 156, 219, 346, 859, 1058, 1067
BspCNI CTCAG 6 cut(s) 277, 343, 829, 1156, 1387, 1555
BspDI ATCGAT 3 cut(s) 929, 1443, 1447
BspEI TCCGGA 1 cut(s) 1029
BspFNI CGCG 4 cut(s) 301, 303, 440, 1079
BspLI GGNNCC 8 cut(s) 184, 214, 272, 746, 831, 905, 1161, 1327
BspMAI CTGCAG 2 cut(s) 994, 1356
BspPI GGATC 2 cut(s) 483, 803
BspT107I GGYRCC 2 cut(s) 212, 829
BspTNI GGTCTC 1 cut(s) 986
BsrBI CCGCTC 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 171
BsrFI RCCGGY 2 cut(s) 215, 686
BsrI ACTGG 2 cut(s) 1122, 1174
BssAI RCCGGY 2 cut(s) 215, 686
BssECI CCNNGG 7 cut(s) 231, 383, 470, 662, 663, 1404, 1429
BssMI GATC 3 cut(s) 475, 808, 1444
BssNI GRCGYC 1 cut(s) 405
BssSI CACGAG 1 cut(s) 1089
BssT1I CCWWGG 1 cut(s) 383
Bst2BI CACGAG 1 cut(s) 1089
Bst2UI CCWGG 4 cut(s) 700, 861, 901, 1430
Bst4CI ACNGT 3 cut(s) 489, 944, 1366
Bst6I CTCTTC 3 cut(s) 111, 133, 979
BstACI GRCGYC 1 cut(s) 405
BstC8I GCNNGC 4 cut(s) 420, 721, 1081, 1492
BstDEI CTNAG 6 cut(s) 264, 351, 837, 1164, 1374, 1542
BstDSI CCRYGG 2 cut(s) 383, 470
BstENI CCTNNNNNAGG 1 cut(s) 1292
BstF5I GGATG 2 cut(s) 589, 718
BstFNI CGCG 4 cut(s) 301, 303, 440, 1079
BstHHI GCGC 1 cut(s) 303
BstKTI GATC 3 cut(s) 478, 811, 1447
BstMAI GTCTC 3 cut(s) 647, 950, 986
BstMBI GATC 3 cut(s) 475, 808, 1444
BstMCI CGRYCG 1 cut(s) 1447
BstMWI GCNNNNNNNGC 8 cut(s) 145, 300, 428, 437, 446, 725, 1064, 1500
BstNI CCWGG 4 cut(s) 700, 861, 901, 1430
BstSCI CCNGG 8 cut(s) 661, 662, 698, 859, 899, 1403, 1404, 1428
BstSFI CTRYAG 2 cut(s) 990, 1352
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 4 cut(s) 301, 303, 440, 1079
BstV1I GCAGC 3 cut(s) 148, 160, 1537
BstV2I GAAGAC 3 cut(s) 34, 229, 506
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
Bsu15I ATCGAT 3 cut(s) 929, 1443, 1447
Bsu36I CCTNAGG 1 cut(s) 1164
BsuRI GGCC 6 cut(s) 156, 219, 346, 859, 1058, 1067
BsuTUI ATCGAT 3 cut(s) 929, 1443, 1447
BtgI CCRYGG 2 cut(s) 383, 470
BtgZI GCGATG 1 cut(s) 858
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 2 cut(s) 589, 718
BtsIMutI CAGTG 2 cut(s) 272, 780
Cac8I GCNNGC 4 cut(s) 420, 721, 1081, 1492
CfoI GCGC 1 cut(s) 303
Cfr10I RCCGGY 2 cut(s) 215, 686
Cfr13I GGNCC 7 cut(s) 182, 271, 689, 744, 903, 1066, 1285
Cfr9I CCCGGG 2 cut(s) 662, 1404
ClaI ATCGAT 3 cut(s) 929, 1443, 1447
CseI GACGC 1 cut(s) 446
CsiI ACCWGGT 1 cut(s) 698
Csp6I GTAC 7 cut(s) 355, 511, 830, 962, 1086, 1378, 1426
CviAII CATG 4 cut(s) 160, 384, 613, 821
CviQI GTAC 7 cut(s) 355, 511, 830, 962, 1086, 1378, 1426
DdeI CTNAG 6 cut(s) 264, 351, 837, 1164, 1374, 1542
DpnI GATC 3 cut(s) 477, 810, 1446
DpnII GATC 3 cut(s) 475, 808, 1444
DraIII CACNNNGTG 1 cut(s) 702
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 4 cut(s) 154, 217, 344, 1056
Eam1104I CTCTTC 3 cut(s) 111, 133, 979
EarI CTCTTC 3 cut(s) 111, 133, 979
EciI GGCGGA 1 cut(s) 886
Eco130I CCWWGG 1 cut(s) 383
Eco147I AGGCCT 1 cut(s) 859
Eco24I GRGCYC 1 cut(s) 1162
Eco31I GGTCTC 1 cut(s) 986
Eco32I GATATC 1 cut(s) 1340
Eco47I GGWCC 6 cut(s) 182, 271, 689, 744, 903, 1285
Eco57I CTGAAG 2 cut(s) 960, 1187
Eco81I CCTNAGG 1 cut(s) 1164
Eco88I CYCGRG 3 cut(s) 289, 662, 1404
EcoNI CCTNNNNNAGG 1 cut(s) 1292
EcoO109I RGGNCCY 2 cut(s) 903, 1285
EcoRI GAATTC 1 cut(s) 1016
EcoRII CCWGG 4 cut(s) 698, 859, 899, 1428
EcoRV GATATC 1 cut(s) 1340
EcoT14I CCWWGG 1 cut(s) 383
EcoT38I GRGCYC 1 cut(s) 1162
ErhI CCWWGG 1 cut(s) 383
FaeI CATG 4 cut(s) 163, 387, 616, 824
FaqI GGGAC 5 cut(s) 449, 468, 730, 1269, 1387
FatI CATG 4 cut(s) 159, 383, 612, 820
FauI CCCGC 4 cut(s) 141, 253, 433, 1422
FblI GTMKAC 2 cut(s) 225, 734
Fnu4HI GCNGC 9 cut(s) 98, 137, 149, 261, 397, 423, 426, 429, 1551
FokI GGATG 2 cut(s) 596, 725
FriOI GRGCYC 1 cut(s) 1162
Fsp4HI GCNGC 9 cut(s) 98, 137, 149, 261, 397, 423, 426, 429, 1551
FspBI CTAG 1 cut(s) 432
GlaI GCGC 1 cut(s) 302
GluI GCNGC 9 cut(s) 98, 137, 149, 261, 397, 423, 426, 429, 1551
GsaI CCCAGC 1 cut(s) 732
GsuI CTGGAG 1 cut(s) 361
HaeIII GGCC 6 cut(s) 156, 219, 346, 859, 1058, 1067
HapII CCGG 5 cut(s) 216, 663, 687, 1030, 1405
HgaI GACGC 1 cut(s) 446
HhaI GCGC 1 cut(s) 303
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 4 cut(s) 163, 387, 616, 824
Hin6I GCGC 1 cut(s) 301
HinP1I GCGC 1 cut(s) 301
HincII GTYRAC 2 cut(s) 18, 226
HindII GTYRAC 2 cut(s) 18, 226
HinfI GANTC 7 cut(s) 200, 276, 287, 629, 1148, 1246, 1529
HpaII CCGG 5 cut(s) 216, 663, 687, 1030, 1405
HphI GGTGA 5 cut(s) 304, 413, 425, 641, 1108
Hpy166II GTNNAC 6 cut(s) 18, 226, 357, 735, 1088, 1116
Hpy188I TCNGA 8 cut(s) 182, 205, 275, 682, 759, 1206, 1337, 1344
Hpy188III TCNNGA 9 cut(s) 692, 911, 1013, 1030, 1136, 1163, 1238, 1280, 1288
Hpy8I GTNNAC 6 cut(s) 18, 226, 357, 735, 1088, 1116
Hpy99I CGWCG 3 cut(s) 227, 332, 410
HpyAV CCTTC 6 cut(s) 202, 593, 601, 917, 1133, 1211
HpyCH4III ACNGT 3 cut(s) 489, 944, 1366
HpyCH4IV ACGT 2 cut(s) 222, 405
HpyCH4V TGCA 6 cut(s) 449, 590, 992, 1231, 1354, 1553
HpyF10VI GCNNNNNNNGC 8 cut(s) 145, 300, 428, 437, 446, 725, 1064, 1500
HpyF3I CTNAG 6 cut(s) 264, 351, 837, 1164, 1374, 1542
HpySE526I ACGT 2 cut(s) 222, 405
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 4 cut(s) 163, 387, 616, 824
HspAI GCGC 1 cut(s) 301
Kpn2I TCCGGA 1 cut(s) 1029
KpnI GGTACC 1 cut(s) 833
Kzo9I GATC 3 cut(s) 475, 808, 1444
LmnI GCTCC 3 cut(s) 144, 380, 1165
Lsp1109I GCAGC 3 cut(s) 148, 160, 1537
LweI GCATC 2 cut(s) 673, 754
MabI ACCWGGT 1 cut(s) 698
MaeI CTAG 1 cut(s) 432
MaeII ACGT 2 cut(s) 222, 405
MaeIII GTNAC 3 cut(s) 401, 951, 1100
MalI GATC 3 cut(s) 477, 810, 1446
MbiI CCGCTC 1 cut(s) 263
MboI GATC 3 cut(s) 475, 808, 1444
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 2 cut(s) 215, 1162
MlsI TGGCCA 2 cut(s) 346, 1058
MluCI AATT 7 cut(s) 306, 542, 1016, 1105, 1208, 1509, 1516
MluNI TGGCCA 2 cut(s) 346, 1058
MlyI GAGTC 2 cut(s) 281, 285
MmeI TCCRAC 4 cut(s) 561, 861, 1385, 1557
Mox20I TGGCCA 2 cut(s) 346, 1058
MroI TCCGGA 1 cut(s) 1029
MroXI GAANNNNTTC 1 cut(s) 999
MscI TGGCCA 2 cut(s) 346, 1058
MseI TTAA 2 cut(s) 567, 1515
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 2 cut(s) 346, 1058
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 5 cut(s) 216, 663, 687, 1030, 1405
MspR9I CCNGG 8 cut(s) 663, 664, 700, 861, 901, 1405, 1406, 1430
Mva1269I GAATGC 1 cut(s) 874
MvaI CCWGG 4 cut(s) 700, 861, 901, 1430
MvnI CGCG 4 cut(s) 301, 303, 440, 1079
MwoI GCNNNNNNNGC 8 cut(s) 145, 300, 428, 437, 446, 725, 1064, 1500
NciI CCSGG 4 cut(s) 663, 664, 1405, 1406
NcoI CCATGG 1 cut(s) 383
NdeII GATC 3 cut(s) 475, 808, 1444
NlaIII CATG 4 cut(s) 163, 387, 616, 824
NlaIV GGNNCC 8 cut(s) 184, 214, 272, 746, 831, 905, 1161, 1327
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 1 cut(s) 401
PceI AGGCCT 1 cut(s) 859
PctI GAATGC 1 cut(s) 874
PdmI GAANNNNTTC 1 cut(s) 999
PfeI GAWTC 5 cut(s) 200, 629, 1148, 1246, 1529
PflMI CCANNNNNTGG 1 cut(s) 384
PkrI GCNGC 9 cut(s) 99, 138, 150, 262, 398, 424, 427, 430, 1552
Ple19I CGATCG 1 cut(s) 1447
PleI GAGTC 2 cut(s) 281, 284
PpsI GAGTC 2 cut(s) 281, 284
PpuMI RGGWCCY 2 cut(s) 903, 1285
Psp5II RGGWCCY 2 cut(s) 903, 1285
Psp6I CCWGG 4 cut(s) 698, 859, 899, 1428
PspFI CCCAGC 1 cut(s) 728
PspGI CCWGG 4 cut(s) 698, 859, 899, 1428
PspN4I GGNNCC 8 cut(s) 184, 214, 272, 746, 831, 905, 1161, 1327
PspPI GGNCC 7 cut(s) 182, 271, 689, 744, 903, 1066, 1285
PspPPI RGGWCCY 2 cut(s) 903, 1285
PsrI GAACNNNNNNTAC 2 cut(s) 503, 535
PstI CTGCAG 2 cut(s) 994, 1356
PsuI RGATCY 1 cut(s) 808
PvuI CGATCG 1 cut(s) 1447
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 7 cut(s) 356, 512, 831, 963, 1087, 1379, 1427
RsaNI GTAC 7 cut(s) 355, 511, 830, 962, 1086, 1378, 1426
RseI CAYNNNNRTG 1 cut(s) 164
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 2 cut(s) 567, 1515
SatI GCNGC 9 cut(s) 98, 137, 149, 261, 397, 423, 426, 429, 1551
Sau3AI GATC 3 cut(s) 475, 808, 1444
Sau96I GGNCC 7 cut(s) 182, 271, 689, 744, 903, 1066, 1285
ScaI AGTACT 1 cut(s) 1379
SchI GAGTC 2 cut(s) 281, 285
ScrFI CCNGG 8 cut(s) 663, 664, 700, 861, 901, 1405, 1406, 1430
SduI GDGCHC 2 cut(s) 215, 1162
SexAI ACCWGGT 1 cut(s) 698
SfaNI GCATC 2 cut(s) 673, 754
SfcI CTRYAG 2 cut(s) 990, 1352
SfiI GGCCNNNNNGGCC 1 cut(s) 1064
SinI GGWCC 6 cut(s) 182, 271, 689, 744, 903, 1285
SmaI CCCGGG 2 cut(s) 664, 1406
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 2 cut(s) 1280, 1381
SmoI CTYRAG 2 cut(s) 1280, 1381
Sse9I AATT 7 cut(s) 306, 542, 1016, 1105, 1208, 1509, 1516
SseBI AGGCCT 1 cut(s) 859
SspI AATATT 1 cut(s) 620
SspMI CTAG 1 cut(s) 432
StuI AGGCCT 1 cut(s) 859
StyD4I CCNGG 8 cut(s) 661, 662, 698, 859, 899, 1403, 1404, 1428
StyI CCWWGG 1 cut(s) 383
TaaI ACNGT 3 cut(s) 489, 944, 1366
TaiI ACGT 2 cut(s) 225, 408
TaqI TCGA 9 cut(s) 225, 929, 1037, 1178, 1269, 1443, 1447, 1454, 1527
TaqII GACCGA 1 cut(s) 223
TasI AATT 7 cut(s) 306, 542, 1016, 1105, 1208, 1509, 1516
TatI WGTACW 4 cut(s) 354, 510, 1085, 1377
TauI GCSGC 6 cut(s) 100, 263, 399, 425, 428, 431
TfiI GAWTC 5 cut(s) 200, 629, 1148, 1246, 1529
Tru1I TTAA 2 cut(s) 567, 1515
Tru9I TTAA 2 cut(s) 567, 1515
TscAI CASTG 2 cut(s) 272, 787
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 3 cut(s) 136, 148, 1550
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 3 cut(s) 17, 729, 1140
TspGWI ACGGA 3 cut(s) 212, 358, 1338
TspMI CCCGGG 2 cut(s) 662, 1404
TspRI CASTG 2 cut(s) 272, 787
Van91I CCANNNNNTGG 1 cut(s) 384
VpaK11BI GGWCC 6 cut(s) 182, 271, 689, 744, 903, 1285
XagI CCTNNNNNAGG 1 cut(s) 1292
XapI RAATTY 2 cut(s) 1016, 1509
XcmI CCANNNNNNNNNTGG 1 cut(s) 797
XmaI CCCGGG 2 cut(s) 662, 1404
XmiI GTMKAC 2 cut(s) 225, 734
XmnI GAANNNNTTC 1 cut(s) 999
XspI CTAG 1 cut(s) 432
ZraI GACGTC 1 cut(s) 406
ZrmI AGTACT 1 cut(s) 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.