Rh5DG401200

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015991
Physical Location & Seq
Reverse (-)
63463479 .. 63466439
2961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG401200.1

Sequence Viewer

Length: 1398 bp
ATGGCCATGTTCCTCCACTTCGGACCCAACACCTTCACCGACTCCGAATGGGGCACCGGCCACGCTGCCCCCTCCATCTTCAACCCAACAAACCTCAACGCCACCCAGTGGGTCAAAGTCGCCAAAGACTCTGGCTTTTCGCGCGTGATCCTCACCGCCAAACACCACGATGGCTTCTGCTTGTGGCCCACCGAGTACACCAACTACTCGGTCAGCTCCAGCCCGTGGAGGAGCGGCGGTGGTGACGTGGTGGGTGACCTCGCTGAGGCTGCTAGGGACGCCGGTATTGGATTGGGCCTTTACCTTTCGCCTTGGGATCGGCACGAGCCGTGTTATGGGAAGACTTTGGAGTACAATGAATTCTATATGGCCCAAATGACAGAGCTCCTCACAAGGTATGGAGATATCAAGGAAGTATGGTTGGATGGTGCAAAAGGGGAAGGGGAGAAGGATATGGATTATTTCTTTGATTCTTGGTTCAGTCTGATTCATCAACACCAGCCAGGCGCTGTCATTTTCTCTGATGCTGGTCCCGACACCAGGTGGGTTGGCGACGAAGCTGGTGTTGCTGGGTCTACTTGTTGGTCTCTTTTCAATAGGAGTTCTGCCAAGATTGGTGACACTGATTTCGAATATTCAAGAGGAGGTGACCCACTTGGTCATGATTGGGTACCAGCTGAGTGTGACGTCTCAATTCGTCCTGGTTGGTTTTGGCATTCATCAGAAGTTCCAAAATCTGCAAGGACTCTGCTTGAACTATACTACAAATCAGTTGGTAGGAACTGTCTCTTGTTACTAAATGTACCCCCAAACTCTTCTGGTCTCATATCAGCAGAAGACATTCAAGTGCTTAAAGAATTCAGTGAGCTCCGAAGATCTATATTCTCACAGAATCTAGCTATGGATGCCATTATTCGTGCGAGCAGTACACGAGGAAGCACCAATAATTCTTGGTTCAATGCCTATAATGTCATGGAAGAAGGTTTTCATTCCTATTGGGCACCTGATGAGAATCAGTCTAATTGGGTTTTATACTTAGATCTTCAAGAACTAGTATCATTCAACATTCTTCAAGTTGAAGAGCCAATTCACATGGGACAGCGGGTTATTGAGTTCCATCTTGAGATCTTGAATGCAGGTGGCAAGTGGAACAAAGTAACTAATGGAACCACTGTGGGATATCGGAGGCTTTTGCAATTTCCAACAGTTAAGTCTCGAAACTTGAGGTTTGTTATTGACAAGTCCCGGGCAGACCCACTGATATCCTATCTGGGTATATATATGGATCCATTCTCCATTTTAAGCAACATATCTCATACAACCACACAGACAGGCATCAATAGTAGTCAAATAATTCACCATATTGAATTCAACCATTCTCAAATTGCTGTATTGTAG

Protein Analysis

465

Amino Acids

52.34

Weight (kDa)

5.3

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha_L_fucos PF01120 25 - 288 4.3e-35 Alpha-L-fucosidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015991)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28100
fragaria_vesca FvH4_3g30940
malus_domestica MD00G1032500.v1.1
prunus_persica Prupe.6G109300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0057471
rosa_laevigata RLG00000035158
rosa_multiflora Rmu_sc0000508.1_g000012 Rmu_sc0003730.1_g000020
rosa_roxburghii Rroxscaffold_1G00022980
rosa_rugosa Rorug05G0307500
rosa_samantha Rh5AG376100 Rh5BG387000 Rh5CG410400 Rh5DG401200
rosa_wichuraiana Rw5G035510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1127
AatII GACGTC 1 cut(s) 690
Acc36I ACCTGC 1 cut(s) 1127
Acc65I GGTACC 1 cut(s) 670
AccB1I GGYRCC 3 cut(s) 53, 670, 1000
AccB7I CCANNNNNTGG 2 cut(s) 108, 225
AccBSI CCGCTC 1 cut(s) 234
AccI GTMKAC 1 cut(s) 575
AccII CGCG 2 cut(s) 142, 144
AciI CCGC 4 cut(s) 156, 234, 237, 1102
AclWI GGATC 4 cut(s) 142, 324, 1280, 1293
AcoI YGGCCR 2 cut(s) 3, 58
AcsI RAATTY 3 cut(s) 359, 857, 1367
AcyI GRCGYC 2 cut(s) 279, 687
AdeI CACNNNGTG 2 cut(s) 108, 543
AfaI GTAC 5 cut(s) 197, 353, 672, 804, 928
AfiI CCNNNNNNNGG 5 cut(s) 108, 225, 265, 335, 540
AhlI ACTAGT 1 cut(s) 1051
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 3 cut(s) 502, 539, 700
AluBI AGCT 6 cut(s) 216, 385, 560, 677, 868, 899
AluI AGCT 6 cut(s) 216, 385, 560, 677, 868, 899
Alw21I GWGCWC 2 cut(s) 387, 870
Alw26I GTCTC 5 cut(s) 591, 694, 791, 827, 1218
AlwI GGATC 4 cut(s) 142, 324, 1280, 1293
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 1245
AoxI GGCC 5 cut(s) 3, 58, 185, 295, 369
ApeKI GCWGC 2 cut(s) 65, 269
ApoI RAATTY 3 cut(s) 359, 857, 1367
ArsI GACNNNNNNTTYG 2 cut(s) 611, 643
Asp700I GAANNNNTTC 2 cut(s) 840, 984
Asp718I GGTACC 1 cut(s) 670
AspLEI GCGC 2 cut(s) 144, 509
AspS9I GGNCC 5 cut(s) 23, 186, 295, 370, 530
AsuC2I CCSGG 2 cut(s) 1246, 1247
AsuHPI GGTGA 7 cut(s) 28, 145, 254, 266, 629, 659, 1349
AsuII TTCGAA 1 cut(s) 630
AvaI CYCGRG 1 cut(s) 1245
AvaII GGWCC 2 cut(s) 23, 530
BaeGI GKGCMC 2 cut(s) 56, 1003
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1285
BanI GGYRCC 3 cut(s) 53, 670, 1000
BanII GRGCYC 2 cut(s) 387, 870
BauI CACGAG 2 cut(s) 323, 930
BbsI GAAGAC 2 cut(s) 347, 843
Bbv12I GWGCWC 2 cut(s) 387, 870
BbvCI CCTCAGC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 52, 256
BccI CCATC 4 cut(s) 83, 164, 419, 1125
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 3 cut(s) 504, 541, 702
BcnI CCSGG 2 cut(s) 1246, 1247
BcoDI GTCTC 5 cut(s) 591, 694, 791, 827, 1218
BcuI ACTAGT 1 cut(s) 1051
BfaI CTAG 3 cut(s) 273, 896, 1052
BfoI RGCGCY 1 cut(s) 510
BfuAI ACCTGC 1 cut(s) 1127
BglII AGATCT 3 cut(s) 875, 1039, 1125
BisI GCNGC 3 cut(s) 66, 235, 270
BlsI GCNGC 3 cut(s) 67, 236, 271
Bme1390I CCNGG 5 cut(s) 504, 541, 702, 1246, 1247
Bme18I GGWCC 2 cut(s) 23, 530
BmeT110I CYCGRG 1 cut(s) 1245
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 5 cut(s) 23, 186, 295, 370, 530
BmiI GGNNCC 7 cut(s) 25, 55, 532, 672, 1002, 1168, 1287
BmrFI CCNGG 5 cut(s) 504, 541, 702, 1246, 1247
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 3 cut(s) 514, 895, 1344
BmuI ACTGGG 1 cut(s) 100
BpiI GAAGAC 2 cut(s) 347, 843
BpmI CTGGAG 1 cut(s) 202
Bpu10I CCTNAGC 1 cut(s) 264
Bpu14I TTCGAA 1 cut(s) 630
BpuEI CTTGAG 2 cut(s) 1142, 1243
BpuMI CCSGG 2 cut(s) 1246, 1247
BsaBI GATNNNNATC 1 cut(s) 1011
BsaHI GRCGYC 2 cut(s) 279, 687
BsaI GGTCTC 2 cut(s) 591, 827
BsaJI CCNNGG 3 cut(s) 224, 311, 1245
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 5 cut(s) 108, 225, 265, 335, 540
Bse118I RCCGGY 2 cut(s) 56, 281
Bse1I ACTGG 1 cut(s) 106
Bse8I GATNNNNATC 1 cut(s) 1011
BseBI CCWGG 3 cut(s) 504, 541, 702
BseDI CCNNGG 3 cut(s) 224, 311, 1245
BseGI GGATG 2 cut(s) 430, 910
BseJI GATNNNNATC 1 cut(s) 1011
BseLI CCNNNNNNNGG 5 cut(s) 108, 225, 265, 335, 540
BseMII CTCAG 2 cut(s) 255, 669
BseNI ACTGG 1 cut(s) 106
BseRI GAGGAG 3 cut(s) 244, 377, 657
BseSI GKGCMC 2 cut(s) 56, 1003
BseXI GCAGC 2 cut(s) 52, 256
BseYI CCCAGC 1 cut(s) 569
Bsh1236I CGCG 2 cut(s) 142, 144
BshFI GGCC 5 cut(s) 5, 60, 187, 297, 371
BshNI GGYRCC 3 cut(s) 53, 670, 1000
BsiHKAI GWGCWC 2 cut(s) 387, 870
BsiHKCI CYCGRG 1 cut(s) 1245
BsiSI CCGG 3 cut(s) 57, 282, 1246
BslFI GGGAC 4 cut(s) 290, 516, 1110, 1228
BslI CCNNNNNNNGG 5 cut(s) 108, 225, 265, 335, 540
BsmAI GTCTC 5 cut(s) 591, 694, 791, 827, 1218
BsmBI CGTCTC 1 cut(s) 694
BsmFI GGGAC 4 cut(s) 290, 516, 1110, 1228
BsmI GAATGC 2 cut(s) 715, 1138
BsnI GGCC 5 cut(s) 5, 60, 187, 297, 371
Bso31I GGTCTC 2 cut(s) 591, 827
BsoBI CYCGRG 1 cut(s) 1245
Bsp119I TTCGAA 1 cut(s) 630
Bsp1286I GDGCHC 4 cut(s) 56, 387, 870, 1003
Bsp143I GATC 6 cut(s) 147, 316, 875, 1039, 1125, 1285
BspACI CCGC 4 cut(s) 156, 234, 237, 1102
BspANI GGCC 5 cut(s) 5, 60, 187, 297, 371
BspCNI CTCAG 2 cut(s) 256, 670
BspFNI CGCG 2 cut(s) 142, 144
BspHI TCATGA 1 cut(s) 661
BspLI GGNNCC 7 cut(s) 25, 55, 532, 672, 1002, 1168, 1287
BspMI ACCTGC 1 cut(s) 1127
BspPI GGATC 4 cut(s) 142, 324, 1280, 1293
BspQI GCTCTTC 1 cut(s) 1074
BspT104I TTCGAA 1 cut(s) 630
BspT107I GGYRCC 3 cut(s) 53, 670, 1000
BspTNI GGTCTC 2 cut(s) 591, 827
BsrBI CCGCTC 1 cut(s) 234
BsrFI RCCGGY 2 cut(s) 56, 281
BsrI ACTGG 1 cut(s) 106
BssAI RCCGGY 2 cut(s) 56, 281
BssECI CCNNGG 3 cut(s) 224, 311, 1245
BssMI GATC 6 cut(s) 147, 316, 875, 1039, 1125, 1285
BssNI GRCGYC 2 cut(s) 279, 687
BssSI CACGAG 2 cut(s) 323, 930
BssT1I CCWWGG 1 cut(s) 311
Bst2BI CACGAG 2 cut(s) 323, 930
Bst2UI CCWGG 3 cut(s) 504, 541, 702
Bst4CI ACNGT 3 cut(s) 785, 1174, 1207
Bst6I CTCTTC 2 cut(s) 820, 1074
BstACI GRCGYC 2 cut(s) 279, 687
BstBI TTCGAA 1 cut(s) 630
BstC8I GCNNGC 1 cut(s) 922
BstDEI CTNAG 3 cut(s) 264, 678, 1036
BstDSI CCRYGG 1 cut(s) 224
BstEII GGTNACC 2 cut(s) 254, 647
BstENI CCTNNNNNAGG 1 cut(s) 263
BstF5I GGATG 2 cut(s) 430, 910
BstFNI CGCG 2 cut(s) 142, 144
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 2 cut(s) 144, 509
BstKTI GATC 6 cut(s) 150, 319, 878, 1042, 1128, 1288
BstMAI GTCTC 5 cut(s) 591, 694, 791, 827, 1218
BstMBI GATC 6 cut(s) 147, 316, 875, 1039, 1125, 1285
BstMWI GCNNNNNNNGC 5 cut(s) 141, 269, 278, 566, 905
BstNI CCWGG 3 cut(s) 504, 541, 702
BstPI GGTNACC 2 cut(s) 254, 647
BstSCI CCNGG 5 cut(s) 502, 539, 700, 1244, 1245
BstSLI GKGCMC 2 cut(s) 56, 1003
BstUI CGCG 2 cut(s) 142, 144
BstV1I GCAGC 2 cut(s) 52, 256
BstV2I GAAGAC 2 cut(s) 347, 843
BstX2I RGATCY 4 cut(s) 875, 1039, 1125, 1285
BstYI RGATCY 4 cut(s) 875, 1039, 1125, 1285
BsuRI GGCC 5 cut(s) 5, 60, 187, 297, 371
BtgI CCRYGG 1 cut(s) 224
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 2 cut(s) 430, 910
BtsIMutI CAGTG 5 cut(s) 113, 621, 868, 1170, 1256
BveI ACCTGC 1 cut(s) 1127
Cac8I GCNNGC 1 cut(s) 922
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 661
CfoI GCGC 2 cut(s) 144, 509
Cfr10I RCCGGY 2 cut(s) 56, 281
Cfr13I GGNCC 5 cut(s) 23, 186, 295, 370, 530
Cfr9I CCCGGG 1 cut(s) 1245
CseI GACGC 1 cut(s) 287
CsiI ACCWGGT 1 cut(s) 539
Csp6I GTAC 5 cut(s) 196, 352, 671, 803, 927
CviAII CATG 4 cut(s) 7, 662, 973, 1093
CviQI GTAC 5 cut(s) 196, 352, 671, 803, 927
DdeI CTNAG 3 cut(s) 264, 678, 1036
DpnI GATC 6 cut(s) 149, 318, 877, 1041, 1127, 1287
DpnII GATC 6 cut(s) 147, 316, 875, 1039, 1125, 1285
DraIII CACNNNGTG 2 cut(s) 108, 543
EaeI YGGCCR 2 cut(s) 3, 58
Eam1104I CTCTTC 2 cut(s) 820, 1074
EarI CTCTTC 2 cut(s) 820, 1074
Ecl136II GAGCTC 2 cut(s) 385, 868
Eco130I CCWWGG 1 cut(s) 311
Eco24I GRGCYC 2 cut(s) 387, 870
Eco31I GGTCTC 2 cut(s) 591, 827
Eco32I GATATC 3 cut(s) 406, 1181, 1263
Eco47I GGWCC 2 cut(s) 23, 530
Eco53kI GAGCTC 2 cut(s) 385, 868
Eco88I CYCGRG 1 cut(s) 1245
Eco91I GGTNACC 2 cut(s) 254, 647
EcoICRI GAGCTC 2 cut(s) 385, 868
EcoNI CCTNNNNNAGG 1 cut(s) 263
EcoO65I GGTNACC 2 cut(s) 254, 647
EcoRI GAATTC 3 cut(s) 359, 857, 1367
EcoRII CCWGG 3 cut(s) 502, 539, 700
EcoRV GATATC 3 cut(s) 406, 1181, 1263
EcoT14I CCWWGG 1 cut(s) 311
EcoT38I GRGCYC 2 cut(s) 387, 870
ErhI CCWWGG 1 cut(s) 311
Esp3I CGTCTC 1 cut(s) 694
FaeI CATG 4 cut(s) 10, 665, 976, 1096
FaqI GGGAC 4 cut(s) 290, 516, 1110, 1228
FatI CATG 4 cut(s) 6, 661, 972, 1092
FauI CCCGC 1 cut(s) 1095
FblI GTMKAC 1 cut(s) 575
Fnu4HI GCNGC 3 cut(s) 66, 235, 270
FokI GGATG 2 cut(s) 437, 917
FriOI GRGCYC 2 cut(s) 387, 870
Fsp4HI GCNGC 3 cut(s) 66, 235, 270
FspBI CTAG 3 cut(s) 273, 896, 1052
GlaI GCGC 2 cut(s) 143, 508
GluI GCNGC 3 cut(s) 66, 235, 270
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 1 cut(s) 202
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 5 cut(s) 5, 60, 187, 297, 371
HapII CCGG 3 cut(s) 57, 282, 1246
HgaI GACGC 1 cut(s) 287
HhaI GCGC 2 cut(s) 144, 509
Hin1I GRCGYC 2 cut(s) 279, 687
Hin1II CATG 4 cut(s) 10, 665, 976, 1096
Hin6I GCGC 2 cut(s) 142, 507
HinP1I GCGC 2 cut(s) 142, 507
HinfI GANTC 7 cut(s) 41, 128, 470, 487, 745, 892, 1012
HpaII CCGG 3 cut(s) 57, 282, 1246
HphI GGTGA 7 cut(s) 28, 145, 254, 266, 629, 659, 1349
Hpy166II GTNNAC 3 cut(s) 198, 576, 929
Hpy188I TCNGA 7 cut(s) 23, 46, 486, 523, 724, 872, 1185
Hpy188III TCNNGA 7 cut(s) 533, 639, 662, 1046, 1121, 1129, 1215
Hpy8I GTNNAC 3 cut(s) 198, 576, 929
Hpy99I CGWCG 1 cut(s) 557
HpyAV CCTTC 4 cut(s) 43, 434, 442, 974
HpyCH4III ACNGT 3 cut(s) 785, 1174, 1207
HpyCH4IV ACGT 2 cut(s) 246, 687
HpyCH4V TGCA 4 cut(s) 431, 740, 1136, 1195
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 269, 278, 566, 905
HpyF3I CTNAG 3 cut(s) 264, 678, 1036
HpySE526I ACGT 2 cut(s) 246, 687
Hsp92I GRCGYC 2 cut(s) 279, 687
Hsp92II CATG 4 cut(s) 10, 665, 976, 1096
HspAI GCGC 2 cut(s) 142, 507
KpnI GGTACC 1 cut(s) 674
Kzo9I GATC 6 cut(s) 147, 316, 875, 1039, 1125, 1285
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 4 cut(s) 221, 231, 390, 873
Lsp1109I GCAGC 2 cut(s) 52, 256
LweI GCATC 3 cut(s) 514, 895, 1344
MabI ACCWGGT 1 cut(s) 539
MaeI CTAG 3 cut(s) 273, 896, 1052
MaeII ACGT 2 cut(s) 246, 687
MaeIII GTNAC 7 cut(s) 242, 254, 617, 647, 683, 792, 1156
MalI GATC 6 cut(s) 149, 318, 877, 1041, 1127, 1287
MbiI CCGCTC 1 cut(s) 234
MboI GATC 6 cut(s) 147, 316, 875, 1039, 1125, 1285
MboII GAAGA 9 cut(s) 70, 352, 807, 848, 885, 989, 1034, 1061, 1091
MflI RGATCY 4 cut(s) 875, 1039, 1125, 1285
MhlI GDGCHC 4 cut(s) 56, 387, 870, 1003
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 35, 122, 739
MmeI TCCRAC 2 cut(s) 402, 1226
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 840, 984
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 852, 1209, 1301
MslI CAYNNNNRTG 2 cut(s) 168, 845
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 677, 1102
MspI CCGG 3 cut(s) 57, 282, 1246
MspR9I CCNGG 5 cut(s) 504, 541, 702, 1246, 1247
Mva1269I GAATGC 2 cut(s) 715, 1138
MvaI CCWGG 3 cut(s) 504, 541, 702
MvnI CGCG 2 cut(s) 142, 144
MwoI GCNNNNNNNGC 5 cut(s) 141, 269, 278, 566, 905
NciI CCSGG 2 cut(s) 1246, 1247
NdeII GATC 6 cut(s) 147, 316, 875, 1039, 1125, 1285
NlaIII CATG 4 cut(s) 10, 665, 976, 1096
NlaIV GGNNCC 7 cut(s) 25, 55, 532, 672, 1002, 1168, 1287
NmuCI GTSAC 5 cut(s) 242, 254, 617, 647, 683
NspV TTCGAA 1 cut(s) 630
PagI TCATGA 1 cut(s) 661
PaqCI CACCTGC 1 cut(s) 1127
PciSI GCTCTTC 1 cut(s) 1074
PctI GAATGC 2 cut(s) 715, 1138
PdmI GAANNNNTTC 2 cut(s) 840, 984
PfeI GAWTC 4 cut(s) 470, 487, 892, 1012
PflMI CCANNNNNTGG 2 cut(s) 108, 225
PkrI GCNGC 3 cut(s) 67, 236, 271
PleI GAGTC 3 cut(s) 35, 122, 739
PpsI GAGTC 3 cut(s) 35, 122, 739
Psp124BI GAGCTC 2 cut(s) 387, 870
Psp6I CCWGG 3 cut(s) 502, 539, 700
PspEI GGTNACC 2 cut(s) 254, 647
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 3 cut(s) 502, 539, 700
PspN4I GGNNCC 7 cut(s) 25, 55, 532, 672, 1002, 1168, 1287
PspPI GGNCC 5 cut(s) 23, 186, 295, 370, 530
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 4 cut(s) 875, 1039, 1125, 1285
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 5 cut(s) 197, 353, 672, 804, 928
RsaNI GTAC 5 cut(s) 196, 352, 671, 803, 927
RseI CAYNNNNRTG 2 cut(s) 168, 845
SacI GAGCTC 2 cut(s) 387, 870
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 3 cut(s) 852, 1209, 1301
SatI GCNGC 3 cut(s) 66, 235, 270
Sau3AI GATC 6 cut(s) 147, 316, 875, 1039, 1125, 1285
Sau96I GGNCC 5 cut(s) 23, 186, 295, 370, 530
SchI GAGTC 3 cut(s) 35, 122, 739
ScrFI CCNGG 5 cut(s) 504, 541, 702, 1246, 1247
SduI GDGCHC 4 cut(s) 56, 387, 870, 1003
SexAI ACCWGGT 1 cut(s) 539
SfaNI GCATC 3 cut(s) 514, 895, 1344
SfuI TTCGAA 1 cut(s) 630
SinI GGWCC 2 cut(s) 23, 530
SmaI CCCGGG 1 cut(s) 1247
SmiMI CAYNNNNRTG 2 cut(s) 168, 845
SmlI CTYRAG 2 cut(s) 1121, 1222
SmoI CTYRAG 2 cut(s) 1121, 1222
SpeI ACTAGT 1 cut(s) 1051
SsiI CCGC 4 cut(s) 156, 234, 237, 1102
SspI AATATT 1 cut(s) 635
SspMI CTAG 3 cut(s) 273, 896, 1052
SstI GAGCTC 2 cut(s) 387, 870
StyD4I CCNGG 5 cut(s) 502, 539, 700, 1244, 1245
StyI CCWWGG 1 cut(s) 311
TaaI ACNGT 3 cut(s) 785, 1174, 1207
TaiI ACGT 2 cut(s) 249, 690
TaqI TCGA 2 cut(s) 630, 1216
TaqII GACCGA 1 cut(s) 199
TatI WGTACW 3 cut(s) 195, 351, 926
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 4 cut(s) 470, 487, 892, 1012
Tru1I TTAA 3 cut(s) 852, 1209, 1301
Tru9I TTAA 3 cut(s) 852, 1209, 1301
TscAI CASTG 5 cut(s) 113, 628, 868, 1177, 1263
TseFI GTSAC 5 cut(s) 242, 254, 617, 647, 683
TseI GCWGC 2 cut(s) 65, 269
Tsp45I GTSAC 5 cut(s) 242, 254, 617, 647, 683
TspDTI ATGAA 4 cut(s) 372, 479, 708, 977
TspMI CCCGGG 1 cut(s) 1245
TspRI CASTG 5 cut(s) 113, 628, 868, 1177, 1263
Van91I CCANNNNNTGG 2 cut(s) 108, 225
VpaK11BI GGWCC 2 cut(s) 23, 530
XagI CCTNNNNNAGG 1 cut(s) 263
XapI RAATTY 3 cut(s) 359, 857, 1367
XmaI CCCGGG 1 cut(s) 1245
XmiI GTMKAC 1 cut(s) 575
XmnI GAANNNNTTC 2 cut(s) 840, 984
XspI CTAG 3 cut(s) 273, 896, 1052
ZraI GACGTC 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.