Rroxscaffold_1G00022980

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015991
Physical Location & Seq
Forward (+)
28433258 .. 28436329
3072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022980.1

Sequence Viewer

Length: 888 bp
ATGGCTTCTGCTTGTGGCCCACCGAGTACACCAACTACTCGGTCAGCTCCAGCCCCCTGGAGGAGCGGCGGTGGTGACGTGGTGGGTGAGCTCGCTGAGGCTGCTAGGGACGCCGGTATTGGATTGGGGCTTTACCTTTCGCCTTGGGATCGGCACGAGCCGTGTTATGGGAAGACTTTGGAGTATAATGAATTCTATATGGCCCAAATGACAGAGCTCCTCACAAGGTATGGAGATATCAAGGAAGTATGGTTGGATGGTGCAAAAGGGGAAGGGGAGAAGGATATGGATTATTTCTTTGATTCTTGGTTCAGTCTGATTCATCAACACCAGCCAGGCGCTGTCATTTTCTCTGATGCTGGTCCCGACACCAGGTGGGTTGGCGACGAAGCTGGTGTTGCTGGGTCTACTTGTTGGTCTCTTTTCAATAGGAGTTCTGCCAAGATTGGTGACACTGATTCTGAATATTCAAGAGGAGGTGACCCACTTGGTCATGATTGGGTACCAGCTGAGTGTGACGTCTCAATTCGTCCTGGTTGGTTTTGGCATTCATCAGAAGTTCCAAAATCTGCAAGGACTCTGCTTGAACTATACTACAAATCAGTTGGTAGGAACTGTCTCTTGTTACTAAATGTACCCCCAAACTCTTCTGGTCTCATATCAGCAGAAGACATTCAAGTGCTTAAAGAATTCAGTGAGCTCCGAAGATCTATATTCTCACAGAATCTAGCTATGGATGCCATTATTCGTGCGAGCAGTACACGAGGAAGCACCAATAATTCTTGGTTCAATGCCTATAATGTCGTGGAAGAAGGTTTTTATTCCTATTGGGCACCTGATGAGAATCAGTCTAATTGGGTTTTATACTTGGATCTTCAAGAGAGCTAG

Protein Analysis

295

Amino Acids

32.79

Weight (kDa)

4.6

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha_L_fucos PF01120 25 - 232 1.4e-17 Alpha-L-fucosidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015991)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28100
fragaria_vesca FvH4_3g30940
malus_domestica MD00G1032500.v1.1
prunus_persica Prupe.6G109300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0057471
rosa_laevigata RLG00000035158
rosa_multiflora Rmu_sc0000508.1_g000012 Rmu_sc0003730.1_g000020
rosa_roxburghii Rroxscaffold_1G00022980
rosa_rugosa Rorug05G0307500
rosa_samantha Rh5AG376100 Rh5BG387000 Rh5CG410400 Rh5DG401200
rosa_wichuraiana Rw5G035510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 522
Acc65I GGTACC 1 cut(s) 502
AccB1I GGYRCC 2 cut(s) 502, 832
AccBSI CCGCTC 1 cut(s) 66
AccI GTMKAC 1 cut(s) 407
AciI CCGC 2 cut(s) 66, 69
AclWI GGATC 2 cut(s) 156, 879
AcsI RAATTY 2 cut(s) 191, 689
AcyI GRCGYC 2 cut(s) 111, 519
AdeI CACNNNGTG 1 cut(s) 375
AfaI GTAC 4 cut(s) 28, 504, 636, 760
AfiI CCNNNNNNNGG 3 cut(s) 60, 167, 372
AgsI TTSAA 6 cut(s) 427, 471, 587, 677, 790, 878
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 4 cut(s) 56, 334, 371, 532
AluBI AGCT 8 cut(s) 47, 91, 217, 392, 509, 700, 731, 885
AluI AGCT 8 cut(s) 47, 91, 217, 392, 509, 700, 731, 885
Alw21I GWGCWC 3 cut(s) 93, 219, 702
Alw26I GTCTC 4 cut(s) 423, 526, 623, 659
AlwI GGATC 2 cut(s) 156, 879
AlwNI CAGNNNCTG 1 cut(s) 341
AoxI GGCC 2 cut(s) 16, 201
ApeKI GCWGC 1 cut(s) 101
ApoI RAATTY 2 cut(s) 191, 689
Asp700I GAANNNNTTC 1 cut(s) 672
Asp718I GGTACC 1 cut(s) 502
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 3 cut(s) 17, 202, 362
AsuHPI GGTGA 4 cut(s) 86, 98, 461, 491
AvaII GGWCC 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 835
BanI GGYRCC 2 cut(s) 502, 832
BanII GRGCYC 3 cut(s) 93, 219, 702
BauI CACGAG 2 cut(s) 155, 762
BbsI GAAGAC 2 cut(s) 179, 675
Bbv12I GWGCWC 3 cut(s) 93, 219, 702
BbvCI CCTCAGC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 88
BccI CCATC 1 cut(s) 251
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 4 cut(s) 58, 336, 373, 534
BcoDI GTCTC 4 cut(s) 423, 526, 623, 659
BfaI CTAG 3 cut(s) 105, 728, 886
BfoI RGCGCY 1 cut(s) 342
BglII AGATCT 1 cut(s) 707
BisI GCNGC 2 cut(s) 67, 102
BlsI GCNGC 2 cut(s) 68, 103
Bme1390I CCNGG 4 cut(s) 58, 336, 373, 534
Bme18I GGWCC 1 cut(s) 362
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 3 cut(s) 17, 202, 362
BmiI GGNNCC 3 cut(s) 364, 504, 834
BmrFI CCNGG 4 cut(s) 58, 336, 373, 534
BmsI GCATC 2 cut(s) 346, 727
BpiI GAAGAC 2 cut(s) 179, 675
BpmI CTGGAG 2 cut(s) 33, 79
Bpu10I CCTNAGC 1 cut(s) 96
BsaBI GATNNNNATC 1 cut(s) 843
BsaHI GRCGYC 2 cut(s) 111, 519
BsaI GGTCTC 2 cut(s) 423, 659
BsaJI CCNNGG 2 cut(s) 56, 143
BsaXI ACNNNNNCTCC 2 cut(s) 55, 85
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 167, 372
Bse118I RCCGGY 1 cut(s) 113
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 4 cut(s) 58, 336, 373, 534
BseDI CCNNGG 2 cut(s) 56, 143
BseGI GGATG 2 cut(s) 262, 742
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 3 cut(s) 60, 167, 372
BseMII CTCAG 2 cut(s) 87, 501
BseRI GAGGAG 3 cut(s) 76, 209, 489
BseSI GKGCMC 1 cut(s) 835
BseXI GCAGC 1 cut(s) 88
BseYI CCCAGC 1 cut(s) 401
BshFI GGCC 2 cut(s) 18, 203
BshNI GGYRCC 2 cut(s) 502, 832
BsiHKAI GWGCWC 3 cut(s) 93, 219, 702
BsiSI CCGG 1 cut(s) 114
BslFI GGGAC 2 cut(s) 122, 348
BslI CCNNNNNNNGG 3 cut(s) 60, 167, 372
BsmAI GTCTC 4 cut(s) 423, 526, 623, 659
BsmBI CGTCTC 1 cut(s) 526
BsmFI GGGAC 2 cut(s) 122, 348
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 2 cut(s) 18, 203
Bso31I GGTCTC 2 cut(s) 423, 659
Bsp1286I GDGCHC 4 cut(s) 93, 219, 702, 835
Bsp143I GATC 3 cut(s) 148, 707, 871
BspACI CCGC 2 cut(s) 66, 69
BspANI GGCC 2 cut(s) 18, 203
BspCNI CTCAG 2 cut(s) 88, 502
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 3 cut(s) 364, 504, 834
BspPI GGATC 2 cut(s) 156, 879
BspT107I GGYRCC 2 cut(s) 502, 832
BspTNI GGTCTC 2 cut(s) 423, 659
BsrBI CCGCTC 1 cut(s) 66
BsrFI RCCGGY 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 2 cut(s) 56, 143
BssMI GATC 3 cut(s) 148, 707, 871
BssNI GRCGYC 2 cut(s) 111, 519
BssSI CACGAG 2 cut(s) 155, 762
BssT1I CCWWGG 1 cut(s) 143
Bst2BI CACGAG 2 cut(s) 155, 762
Bst2UI CCWGG 4 cut(s) 58, 336, 373, 534
Bst4CI ACNGT 1 cut(s) 617
Bst6I CTCTTC 1 cut(s) 652
BstACI GRCGYC 2 cut(s) 111, 519
BstC8I GCNNGC 2 cut(s) 93, 754
BstDEI CTNAG 2 cut(s) 96, 510
BstEII GGTNACC 1 cut(s) 479
BstF5I GGATG 2 cut(s) 262, 742
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 3 cut(s) 151, 710, 874
BstMAI GTCTC 4 cut(s) 423, 526, 623, 659
BstMBI GATC 3 cut(s) 148, 707, 871
BstMWI GCNNNNNNNGC 4 cut(s) 101, 110, 398, 737
BstNI CCWGG 4 cut(s) 58, 336, 373, 534
BstPI GGTNACC 1 cut(s) 479
BstSCI CCNGG 4 cut(s) 56, 334, 371, 532
BstSLI GKGCMC 1 cut(s) 835
BstV1I GCAGC 1 cut(s) 88
BstV2I GAAGAC 2 cut(s) 179, 675
BstX2I RGATCY 2 cut(s) 707, 871
BstXI CCANNNNNNTGG 1 cut(s) 57
BstYI RGATCY 2 cut(s) 707, 871
BsuRI GGCC 2 cut(s) 18, 203
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 2 cut(s) 262, 742
BtsIMutI CAGTG 2 cut(s) 453, 700
Cac8I GCNNGC 2 cut(s) 93, 754
CaiI CAGNNNCTG 1 cut(s) 341
CciI TCATGA 1 cut(s) 493
CfoI GCGC 1 cut(s) 341
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 3 cut(s) 17, 202, 362
CseI GACGC 1 cut(s) 119
CsiI ACCWGGT 1 cut(s) 371
Csp6I GTAC 4 cut(s) 27, 503, 635, 759
CviAII CATG 1 cut(s) 494
CviQI GTAC 4 cut(s) 27, 503, 635, 759
DdeI CTNAG 2 cut(s) 96, 510
DpnI GATC 3 cut(s) 150, 709, 873
DpnII GATC 3 cut(s) 148, 707, 871
DraIII CACNNNGTG 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 652
EarI CTCTTC 1 cut(s) 652
Ecl136II GAGCTC 3 cut(s) 91, 217, 700
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 3 cut(s) 93, 219, 702
Eco31I GGTCTC 2 cut(s) 423, 659
Eco32I GATATC 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 362
Eco53kI GAGCTC 3 cut(s) 91, 217, 700
Eco91I GGTNACC 1 cut(s) 479
EcoICRI GAGCTC 3 cut(s) 91, 217, 700
EcoO65I GGTNACC 1 cut(s) 479
EcoRI GAATTC 2 cut(s) 191, 689
EcoRII CCWGG 4 cut(s) 56, 334, 371, 532
EcoRV GATATC 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 3 cut(s) 93, 219, 702
ErhI CCWWGG 1 cut(s) 143
Esp3I CGTCTC 1 cut(s) 526
FaeI CATG 1 cut(s) 497
FaqI GGGAC 2 cut(s) 122, 348
FatI CATG 1 cut(s) 493
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 2 cut(s) 67, 102
FokI GGATG 2 cut(s) 269, 749
FriOI GRGCYC 3 cut(s) 93, 219, 702
Fsp4HI GCNGC 2 cut(s) 67, 102
FspBI CTAG 3 cut(s) 105, 728, 886
GlaI GCGC 1 cut(s) 340
GluI GCNGC 2 cut(s) 67, 102
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 2 cut(s) 33, 79
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 2 cut(s) 18, 203
HapII CCGG 1 cut(s) 114
HgaI GACGC 1 cut(s) 119
HhaI GCGC 1 cut(s) 341
Hin1I GRCGYC 2 cut(s) 111, 519
Hin1II CATG 1 cut(s) 497
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HinfI GANTC 6 cut(s) 302, 319, 458, 577, 724, 844
HpaII CCGG 1 cut(s) 114
HphI GGTGA 4 cut(s) 86, 98, 461, 491
Hpy166II GTNNAC 3 cut(s) 29, 408, 761
Hpy188I TCNGA 5 cut(s) 318, 355, 463, 556, 704
Hpy188III TCNNGA 4 cut(s) 365, 471, 494, 878
Hpy8I GTNNAC 3 cut(s) 29, 408, 761
Hpy99I CGWCG 1 cut(s) 389
HpyAV CCTTC 3 cut(s) 266, 274, 806
HpyCH4III ACNGT 1 cut(s) 617
HpyCH4IV ACGT 2 cut(s) 78, 519
HpyCH4V TGCA 2 cut(s) 263, 572
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 110, 398, 737
HpyF3I CTNAG 2 cut(s) 96, 510
HpySE526I ACGT 2 cut(s) 78, 519
Hsp92I GRCGYC 2 cut(s) 111, 519
Hsp92II CATG 1 cut(s) 497
HspAI GCGC 1 cut(s) 339
KpnI GGTACC 1 cut(s) 506
Kzo9I GATC 3 cut(s) 148, 707, 871
LmnI GCTCC 4 cut(s) 52, 63, 222, 705
Lsp1109I GCAGC 1 cut(s) 88
LweI GCATC 2 cut(s) 346, 727
MabI ACCWGGT 1 cut(s) 371
MaeI CTAG 3 cut(s) 105, 728, 886
MaeII ACGT 2 cut(s) 78, 519
MaeIII GTNAC 5 cut(s) 74, 449, 479, 515, 624
MalI GATC 3 cut(s) 150, 709, 873
MbiI CCGCTC 1 cut(s) 66
MboI GATC 3 cut(s) 148, 707, 871
MboII GAAGA 6 cut(s) 184, 639, 680, 717, 821, 866
MflI RGATCY 2 cut(s) 707, 871
MhlI GDGCHC 4 cut(s) 93, 219, 702, 835
MluCI AATT 5 cut(s) 191, 525, 689, 778, 853
MlyI GAGTC 1 cut(s) 571
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 6 cut(s) 54, 91, 230, 467, 470, 758
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 1 cut(s) 684
MslI CAYNNNNRTG 1 cut(s) 677
MspA1I CMGCKG 1 cut(s) 509
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 4 cut(s) 58, 336, 373, 534
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 4 cut(s) 58, 336, 373, 534
MwoI GCNNNNNNNGC 4 cut(s) 101, 110, 398, 737
NdeII GATC 3 cut(s) 148, 707, 871
NlaIII CATG 1 cut(s) 497
NlaIV GGNNCC 3 cut(s) 364, 504, 834
NmuCI GTSAC 4 cut(s) 74, 449, 479, 515
PagI TCATGA 1 cut(s) 493
PctI GAATGC 1 cut(s) 547
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 5 cut(s) 302, 319, 458, 724, 844
PkrI GCNGC 2 cut(s) 68, 103
PleI GAGTC 1 cut(s) 571
PpsI GAGTC 1 cut(s) 571
Psp124BI GAGCTC 3 cut(s) 93, 219, 702
Psp6I CCWGG 4 cut(s) 56, 334, 371, 532
PspEI GGTNACC 1 cut(s) 479
PspFI CCCAGC 1 cut(s) 401
PspGI CCWGG 4 cut(s) 56, 334, 371, 532
PspN4I GGNNCC 3 cut(s) 364, 504, 834
PspPI GGNCC 3 cut(s) 17, 202, 362
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 2 cut(s) 707, 871
PvuII CAGCTG 1 cut(s) 509
RsaI GTAC 4 cut(s) 28, 504, 636, 760
RsaNI GTAC 4 cut(s) 27, 503, 635, 759
RseI CAYNNNNRTG 1 cut(s) 677
SacI GAGCTC 3 cut(s) 93, 219, 702
SaqAI TTAA 1 cut(s) 684
SatI GCNGC 2 cut(s) 67, 102
Sau3AI GATC 3 cut(s) 148, 707, 871
Sau96I GGNCC 3 cut(s) 17, 202, 362
SchI GAGTC 1 cut(s) 571
ScrFI CCNGG 4 cut(s) 58, 336, 373, 534
SduI GDGCHC 4 cut(s) 93, 219, 702, 835
SexAI ACCWGGT 1 cut(s) 371
SfaNI GCATC 2 cut(s) 346, 727
SinI GGWCC 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 677
Sse9I AATT 5 cut(s) 191, 525, 689, 778, 853
SsiI CCGC 2 cut(s) 66, 69
SspI AATATT 1 cut(s) 467
SspMI CTAG 3 cut(s) 105, 728, 886
SstI GAGCTC 3 cut(s) 93, 219, 702
StyD4I CCNGG 4 cut(s) 56, 334, 371, 532
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 1 cut(s) 617
TaiI ACGT 2 cut(s) 81, 522
TaqII GACCGA 1 cut(s) 30
TasI AATT 5 cut(s) 191, 525, 689, 778, 853
TatI WGTACW 2 cut(s) 26, 758
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 5 cut(s) 302, 319, 458, 724, 844
Tru1I TTAA 1 cut(s) 684
Tru9I TTAA 1 cut(s) 684
TscAI CASTG 2 cut(s) 460, 700
TseFI GTSAC 4 cut(s) 74, 449, 479, 515
TseI GCWGC 1 cut(s) 101
Tsp45I GTSAC 4 cut(s) 74, 449, 479, 515
TspDTI ATGAA 3 cut(s) 204, 311, 540
TspRI CASTG 2 cut(s) 460, 700
VpaK11BI GGWCC 1 cut(s) 362
XapI RAATTY 2 cut(s) 191, 689
XmiI GTMKAC 1 cut(s) 407
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 3 cut(s) 105, 728, 886
ZraI GACGTC 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.