MD00G1065600.v1.1

Major intrinsic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0020378
Physical Location & Seq
Forward (+)
12625233 .. 12629439
4207 bp
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UTR
Exon/CDS
Intron
MD00G1065600.v1.1.491

Sequence Viewer

Length: 222 bp
ATGTGTGCCTCTGTGGGCATCCAAGGAAGTGCTTGGGCCTTTGGTGGGACCATCTTTGCCTTTGTTTACTCCAATGCTGGTATCTCAGGAGGGCACATCAACCCAGCAGTGACCTTTGGGCTCTTCATGGCAAGGAAACTCTCCCTTACCAAAGCTGTTTTCTACATAGTGATGCAGACCGTTGGTGTAATTGCTGGTGCTGGTGTTGTGAAGGGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0008150 GO:0016020 GO:0016021 GO:0031224 GO:0031226 GO:0034220 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

74

Amino Acids

7.46

Weight (kDa)

9.86

Isoelectric Point (pI)

13.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 5 - 72 8.1e-23 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020378)

Species Orthologous Gene IDs
malus_domestica MD00G1065600.v1.1 MD02G1111400.v1.1
rosa_chinensis RchiOBHm_Chr2g0098701
rosa_multiflora Rmu_co8065218.1_g000001
rosa_samantha Rh2BG125400 Rh2DG127500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 1 cut(s) 45
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 1 cut(s) 155
AluI AGCT 1 cut(s) 155
AoxI GGCC 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 215
AspS9I GGNCC 2 cut(s) 36, 48
AvaII GGWCC 1 cut(s) 48
BaeGI GKGCMC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 123
BccI CCATC 1 cut(s) 59
BfaI CTAG 1 cut(s) 220
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 2 cut(s) 36, 48
BmiI GGNNCC 1 cut(s) 49
BmsI GCATC 2 cut(s) 27, 162
BsaJI CCNNGG 1 cut(s) 22
Bsc4I CCNNNNNNNGG 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 22
BseGI GGATG 1 cut(s) 18
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 96
BseYI CCCAGC 1 cut(s) 103
BshFI GGCC 1 cut(s) 38
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 96, 123
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 98
BspLI GGNNCC 1 cut(s) 49
BspQI GCTCTTC 1 cut(s) 128
BssECI CCNNGG 1 cut(s) 22
BssT1I CCWWGG 1 cut(s) 22
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 1 cut(s) 128
BstDEI CTNAG 1 cut(s) 85
BstF5I GGATG 1 cut(s) 18
BstSLI GKGCMC 1 cut(s) 96
BsuRI GGCC 1 cut(s) 38
BtsCI GGATG 1 cut(s) 18
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 114
Cfr13I GGNCC 2 cut(s) 36, 48
CviAII CATG 1 cut(s) 127
CviJI RGCY 4 cut(s) 38, 121, 155, 216
CviKI_1 RGCY 4 cut(s) 38, 121, 155, 216
DdeI CTNAG 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco130I CCWWGG 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 22
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 1 cut(s) 130
FaiI YATR 2 cut(s) 128, 167
FaqI GGGAC 1 cut(s) 61
FatI CATG 1 cut(s) 126
FokI GGATG 1 cut(s) 5
FriOI GRGCYC 1 cut(s) 123
FspBI CTAG 1 cut(s) 220
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 1 cut(s) 38
Hin1II CATG 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 67
Hpy188III TCNNGA 1 cut(s) 87
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4V TGCA 1 cut(s) 175
HpyF3I CTNAG 1 cut(s) 85
Hsp92II CATG 1 cut(s) 130
LguI GCTCTTC 1 cut(s) 128
LpnPI CCDG 5 cut(s) 63, 72, 117, 180, 186
LweI GCATC 2 cut(s) 27, 162
MaeI CTAG 1 cut(s) 220
MaeIII GTNAC 1 cut(s) 109
MboII GAAGA 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 96, 123
MluCI AATT 1 cut(s) 189
MnlI CCTC 2 cut(s) 19, 83
MroXI GAANNNNTTC 1 cut(s) 215
MslI CAYNNNNRTG 1 cut(s) 170
NlaIII CATG 1 cut(s) 130
NlaIV GGNNCC 1 cut(s) 49
NmuCI GTSAC 1 cut(s) 109
PciSI GCTCTTC 1 cut(s) 128
PdmI GAANNNNTTC 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 49
PspPI GGNCC 2 cut(s) 36, 48
RseI CAYNNNNRTG 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 128
Sau96I GGNCC 2 cut(s) 36, 48
SduI GDGCHC 2 cut(s) 96, 123
SetI ASST 2 cut(s) 116, 157
SfaNI GCATC 2 cut(s) 27, 162
SgeI CNNG 9 cut(s) 35, 45, 90, 99, 116, 139, 144, 207, 213
SinI GGWCC 1 cut(s) 48
SmiMI CAYNNNNRTG 1 cut(s) 170
Sse9I AATT 1 cut(s) 189
SspMI CTAG 1 cut(s) 220
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 1 cut(s) 181
TasI AATT 1 cut(s) 189
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 1 cut(s) 109
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 115
TspRI CASTG 1 cut(s) 114
VpaK11BI GGWCC 1 cut(s) 48
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.