Rh2BG125400

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0020378
Physical Location & Seq
Reverse (-)
10706788 .. 10707284
497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG125400.1

Sequence Viewer

Length: 402 bp
ATGTCACGGTTGCCACCATCATCGGACACAAGAAGGAAGCTGCTTAGTGTGACGGCGTTGGTGTTCTTGGCATTAATTGCCTGGGCCTTCGGTGGCATGATCTTCATCCTGGTTTACTCCATCGCTAGAATCTCAGGCGGTCACATCAACCCTGCGGTGACTTTCGGGCTGTTGGTTGTGAGGAGGGTGTCACTGCTCCGAACAGTGGCTTACATTGTTGCACAATGCTTAGGAGGCATATGCGGCGTAGGGTTAGTGAAAGCTTTGATGAAGCATTACTACCTCACGCATGGAGGTGGTGCCAACTCGGTTGCTCCTGATTATTCCAAAGGCGCTGCTTTGGGAGCTGAAATAGTTGGCACTTTTCTTCTGGTCTACAGTCTTCTCTGGAACAGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

133

Amino Acids

14.2

Weight (kDa)

10.03

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 22 - 128 8.2e-35 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020378)

Species Orthologous Gene IDs
malus_domestica MD00G1065600.v1.1 MD02G1111400.v1.1
rosa_chinensis RchiOBHm_Chr2g0098701
rosa_multiflora Rmu_co8065218.1_g000001
rosa_samantha Rh2BG125400 Rh2DG127500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 375
AciI CCGC 3 cut(s) 138, 155, 243
AfiI CCNNNNNNNGG 1 cut(s) 205
AjnI CCWGG 2 cut(s) 80, 108
AluBI AGCT 3 cut(s) 40, 263, 347
AluI AGCT 3 cut(s) 40, 263, 347
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 2 cut(s) 40, 335
AseI ATTAAT 1 cut(s) 74
AspLEI GCGC 1 cut(s) 335
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 169
BanI GGYRCC 1 cut(s) 299
BarI GAAGNNNNNNTAC 2 cut(s) 263, 295
BbsI GAAGAC 1 cut(s) 374
BbvI GCAGC 2 cut(s) 27, 322
BccI CCATC 2 cut(s) 25, 128
BceAI ACGGC 1 cut(s) 69
BciT130I CCWGG 2 cut(s) 82, 110
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 1 cut(s) 376
BfoI RGCGCY 1 cut(s) 336
BisI GCNGC 3 cut(s) 41, 244, 336
BlsI GCNGC 3 cut(s) 42, 245, 337
Bme1390I CCNGG 2 cut(s) 82, 110
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 301
BmrFI CCNGG 2 cut(s) 82, 110
BmsI GCATC 1 cut(s) 386
BpiI GAAGAC 1 cut(s) 374
Bpu10I CCTNAGC 1 cut(s) 229
BsaBI GATNNNNATC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 81
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse8I GATNNNNATC 1 cut(s) 104
BseBI CCWGG 2 cut(s) 82, 110
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 1 cut(s) 105
BseJI GATNNNNATC 1 cut(s) 104
BseLI CCNNNNNNNGG 1 cut(s) 205
BseMII CTCAG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 196
BseXI GCAGC 2 cut(s) 27, 322
BshFI GGCC 1 cut(s) 86
BshNI GGYRCC 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 205
BsnI GGCC 1 cut(s) 86
Bsp143I GATC 1 cut(s) 99
BspACI CCGC 3 cut(s) 138, 155, 243
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 1 cut(s) 146
BspLI GGNNCC 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 1 cut(s) 99
Bst2UI CCWGG 2 cut(s) 82, 110
Bst4CI ACNGT 3 cut(s) 9, 205, 380
BstAPI GCANNNNNTGC 1 cut(s) 77
BstDEI CTNAG 3 cut(s) 44, 133, 229
BstF5I GGATG 1 cut(s) 105
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 1 cut(s) 102
BstMBI GATC 1 cut(s) 99
BstMWI GCNNNNNNNGC 4 cut(s) 77, 234, 243, 344
BstNI CCWGG 2 cut(s) 82, 110
BstSCI CCNGG 2 cut(s) 80, 108
BstSFI CTRYAG 1 cut(s) 376
BstV1I GCAGC 2 cut(s) 27, 322
BstV2I GAAGAC 1 cut(s) 374
BsuRI GGCC 1 cut(s) 86
BtgZI GCGATG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 105
BtsI GCAGTG 1 cut(s) 191
BtsIMutI CAGTG 2 cut(s) 191, 210
CfoI GCGC 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 84
CviAII CATG 2 cut(s) 97, 290
CviJI RGCY 6 cut(s) 40, 86, 169, 209, 263, 347
CviKI_1 RGCY 6 cut(s) 40, 86, 169, 209, 263, 347
DdeI CTNAG 3 cut(s) 44, 133, 229
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
EcoRII CCWGG 2 cut(s) 80, 108
FaeI CATG 2 cut(s) 100, 293
FaiI YATR 4 cut(s) 98, 239, 241, 291
FatI CATG 2 cut(s) 96, 289
FauNDI CATATG 1 cut(s) 239
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 3 cut(s) 41, 244, 336
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 3 cut(s) 41, 244, 336
FspBI CTAG 1 cut(s) 126
GlaI GCGC 1 cut(s) 334
GluI GCNGC 3 cut(s) 41, 244, 336
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 1 cut(s) 86
HhaI GCGC 1 cut(s) 335
Hin1II CATG 2 cut(s) 100, 293
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 1 cut(s) 129
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 2 cut(s) 115, 376
Hpy188I TCNGA 2 cut(s) 25, 200
Hpy188III TCNNGA 2 cut(s) 317, 388
Hpy8I GTNNAC 2 cut(s) 115, 376
HpyAV CCTTC 2 cut(s) 27, 97
HpyCH4III ACNGT 3 cut(s) 9, 205, 380
HpyCH4V TGCA 1 cut(s) 221
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 234, 243, 344
HpyF3I CTNAG 3 cut(s) 44, 133, 229
Hsp92II CATG 2 cut(s) 100, 293
HspAI GCGC 1 cut(s) 333
Kzo9I GATC 1 cut(s) 99
LmnI GCTCC 3 cut(s) 201, 319, 344
LpnPI CCDG 9 cut(s) 67, 94, 95, 120, 122, 165, 330, 356, 373
Lsp1109I GCAGC 2 cut(s) 27, 322
LweI GCATC 1 cut(s) 386
MaeI CTAG 1 cut(s) 126
MaeIII GTNAC 5 cut(s) 3, 49, 140, 157, 189
MalI GATC 1 cut(s) 101
MboI GATC 1 cut(s) 99
MboII GAAGA 3 cut(s) 94, 359, 374
MluCI AATT 1 cut(s) 75
MnlI CCTC 5 cut(s) 174, 177, 227, 287, 293
MseI TTAA 1 cut(s) 74
MslI CAYNNNNRTG 1 cut(s) 294
MspR9I CCNGG 2 cut(s) 82, 110
MvaI CCWGG 2 cut(s) 82, 110
MwoI GCNNNNNNNGC 4 cut(s) 77, 234, 243, 344
NdeI CATATG 1 cut(s) 239
NdeII GATC 1 cut(s) 99
NlaIII CATG 2 cut(s) 100, 293
NlaIV GGNNCC 1 cut(s) 301
NmuCI GTSAC 5 cut(s) 3, 49, 140, 157, 189
PfeI GAWTC 1 cut(s) 129
PkrI GCNGC 3 cut(s) 42, 245, 337
PshBI ATTAAT 1 cut(s) 74
Psp6I CCWGG 2 cut(s) 80, 108
PspGI CCWGG 2 cut(s) 80, 108
PspN4I GGNNCC 1 cut(s) 301
PspPI GGNCC 1 cut(s) 84
RseI CAYNNNNRTG 1 cut(s) 294
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 3 cut(s) 41, 244, 336
Sau3AI GATC 1 cut(s) 99
Sau96I GGNCC 1 cut(s) 84
ScrFI CCNGG 2 cut(s) 82, 110
SetI ASST 5 cut(s) 42, 265, 285, 298, 349
SfaNI GCATC 1 cut(s) 386
SfcI CTRYAG 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 294
Sse9I AATT 1 cut(s) 75
SsiI CCGC 3 cut(s) 138, 155, 243
SspMI CTAG 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 80, 108
TaaI ACNGT 3 cut(s) 9, 205, 380
TasI AATT 1 cut(s) 75
TauI GCSGC 1 cut(s) 246
TfiI GAWTC 1 cut(s) 129
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 2 cut(s) 198, 210
TseFI GTSAC 5 cut(s) 3, 49, 140, 157, 189
TseI GCWGC 2 cut(s) 40, 335
Tsp45I GTSAC 5 cut(s) 3, 49, 140, 157, 189
TspDTI ATGAA 2 cut(s) 94, 284
TspRI CASTG 2 cut(s) 198, 210
VspI ATTAAT 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 375
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.