MD00G1095400.v1.1

DUF3700

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0017391
Physical Location & Seq
Forward (+)
19920238 .. 19922067
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1095400.v1.1.491

Sequence Viewer

Length: 780 bp
ATGTTGACGGCTTTTGAGAAAGCGGTCGGAAAACCGCCCGAGGAGCTGAGGGTTCCTTCGAATGTGGTGGAAAAGGCAAAGACCAGAGAGGAGATTTTGGAGATTTTCCAGTCGTCGTGTCCCGAGTCCACTGTTTACCACCTTTCTAATGGAAGTTTCATGGCTTTGTCACAGGACAATGAGAGTGTTCAGCCACACCCAAGGTGCATTGTTGTCCTAGACGATGTCTTCTGCATTTTTATAGGCACTTTGGAAAACACTTCTGACCTGAGACGGCACTACGGCCTTCCAAGACAAGCAACAGAAGCTATGGTCGTTGTTGAAGCCTATAAAGTCCTAAGAGATCGCGCACCCTACCCTCCGGATCAAGTCATCAAAGATCTACAAGGAAAATTCGCATTTATTCTCTTCGATGCTAGAACTGATACACTTTTTGCTGCCAGGGACCGAGATGGAAGCGTGGAGCTTCACTGGGGAATGGCAGGTGATGGATCCCTAGTCTGCTCACACAATCTTGACATCATCATCGAGGCTTGTGGAAAATTTTGTTCACCTTTCCCTCCAGGTTGCATATTCACAAGTGGAAGTGGGTTGATGAGCTTTGTTCATCCAATGCACAAGGTAAGGGCAAGTAGGCGTGAAGACGACAATGGACAAGTTTGTGGAGTGACGTTTCAGGTGGATCTTTACACGCGAATCCCCAGCATTCCGCGCACTGGTAGTGCTTCAAATTGGGCCGACATAACTCTTGTCAAGGAGGAGAATAACATTCATCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

260

Amino Acids

28.82

Weight (kDa)

5.49

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 2 - 227 2e-88 Aluminium induced protein
GATase_6 PF13522 60 - 166 3.3e-06 Glutamine amidotransferase domain
GATase_7 PF13537 74 - 169 9.8e-10 Glutamine amidotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017391)

Species Orthologous Gene IDs
malus_domestica MD00G1095400.v1.1
prunus_persica Prupe.4G045300_v2.0.a1
pyrus_communis pycom10g24780
rosa_chinensis RchiOBHm_Chr5g0008201
rosa_laevigata RLG00000031546
rosa_multiflora Rmu_sc0001626.1_g000020 Rmu_sc0035773.1_g000001
rosa_rugosa Rorug04G0434200.1
rosa_samantha Rh5AG062300 Rh5BG058800 Rh5CG069300 Rh5DG058200
rosa_wichuraiana Rw5G005840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 1 cut(s) 473
AccII CGCG 3 cut(s) 348, 694, 712
AccIII TCCGGA 1 cut(s) 361
AciI CCGC 3 cut(s) 23, 35, 710
AclWI GGATC 4 cut(s) 372, 486, 499, 690
AcsI RAATTY 2 cut(s) 392, 542
AfiI CCNNNNNNNGG 1 cut(s) 716
AgsI TTSAA 2 cut(s) 323, 729
AjnI CCWGG 2 cut(s) 440, 562
AluBI AGCT 4 cut(s) 46, 308, 466, 600
AluI AGCT 4 cut(s) 46, 308, 466, 600
Alw26I GTCTC 1 cut(s) 265
AlwI GGATC 4 cut(s) 372, 486, 499, 690
Ama87I CYCGRG 2 cut(s) 38, 122
Aor13HI TCCGGA 1 cut(s) 361
AoxI GGCC 2 cut(s) 283, 735
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 2 cut(s) 392, 542
AspLEI GCGC 2 cut(s) 350, 714
AspS9I GGNCC 2 cut(s) 445, 735
AsuHPI GGTGA 2 cut(s) 497, 543
AsuII TTCGAA 1 cut(s) 59
AvaI CYCGRG 2 cut(s) 38, 122
AvaII GGWCC 1 cut(s) 445
BamHI GGATCC 1 cut(s) 491
BbsI GAAGAC 2 cut(s) 220, 648
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 424
BccI CCATC 2 cut(s) 446, 482
BceAI ACGGC 3 cut(s) 24, 290, 298
BciT130I CCWGG 2 cut(s) 442, 564
BcoDI GTCTC 1 cut(s) 265
BfaI CTAG 3 cut(s) 218, 417, 497
BfuAI ACCTGC 1 cut(s) 473
BglII AGATCT 1 cut(s) 379
BisI GCNGC 1 cut(s) 438
BlsI GCNGC 1 cut(s) 439
Bme1390I CCNGG 2 cut(s) 442, 564
Bme18I GGWCC 1 cut(s) 445
BmeT110I CYCGRG 2 cut(s) 38, 122
BmgT120I GGNCC 2 cut(s) 445, 735
BmiI GGNNCC 3 cut(s) 54, 446, 493
BmrFI CCNGG 2 cut(s) 442, 564
BmrI ACTGGG 1 cut(s) 481
BmsI GCATC 1 cut(s) 403
BmuI ACTGGG 1 cut(s) 481
BpiI GAAGAC 2 cut(s) 220, 648
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 47
Bpu14I TTCGAA 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 39, 200, 441
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 1 cut(s) 716
Bse1I ACTGG 3 cut(s) 109, 476, 721
BseAI TCCGGA 1 cut(s) 361
BseBI CCWGG 2 cut(s) 442, 564
BseDI CCNNGG 3 cut(s) 39, 200, 441
BseGI GGATG 2 cut(s) 607, 772
BseLI CCNNNNNNNGG 1 cut(s) 716
BseMII CTCAG 2 cut(s) 38, 260
BseNI ACTGG 3 cut(s) 109, 476, 721
BseRI GAGGAG 3 cut(s) 56, 104, 773
BseXI GCAGC 1 cut(s) 424
BseYI CCCAGC 1 cut(s) 701
Bsh1236I CGCG 3 cut(s) 348, 694, 712
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 2 cut(s) 285, 737
BsiEI CGRYCG 1 cut(s) 27
BsiHKCI CYCGRG 2 cut(s) 38, 122
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 2 cut(s) 105, 458
BslI CCNNNNNNNGG 1 cut(s) 716
BsmAI GTCTC 1 cut(s) 265
BsmBI CGTCTC 1 cut(s) 265
BsmFI GGGAC 2 cut(s) 105, 458
BsmI GAATGC 1 cut(s) 705
BsnI GGCC 2 cut(s) 285, 737
BsoBI CYCGRG 2 cut(s) 38, 122
Bsp119I TTCGAA 1 cut(s) 59
Bsp13I TCCGGA 1 cut(s) 361
Bsp143I GATC 5 cut(s) 343, 364, 379, 491, 682
BspACI CCGC 3 cut(s) 23, 35, 710
BspANI GGCC 2 cut(s) 285, 737
BspCNI CTCAG 2 cut(s) 39, 261
BspEI TCCGGA 1 cut(s) 361
BspFNI CGCG 3 cut(s) 348, 694, 712
BspLI GGNNCC 3 cut(s) 54, 446, 493
BspMI ACCTGC 1 cut(s) 473
BspPI GGATC 4 cut(s) 372, 486, 499, 690
BspT104I TTCGAA 1 cut(s) 59
BsrI ACTGG 3 cut(s) 109, 476, 721
BssECI CCNNGG 3 cut(s) 39, 200, 441
BssMI GATC 5 cut(s) 343, 364, 379, 491, 682
BssT1I CCWWGG 1 cut(s) 200
Bst2UI CCWGG 2 cut(s) 442, 564
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 1 cut(s) 413
BstBI TTCGAA 1 cut(s) 59
BstDEI CTNAG 3 cut(s) 47, 269, 338
BstF5I GGATG 2 cut(s) 607, 772
BstFNI CGCG 3 cut(s) 348, 694, 712
BstHHI GCGC 2 cut(s) 350, 714
BstKTI GATC 5 cut(s) 346, 367, 382, 494, 685
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 5 cut(s) 343, 364, 379, 491, 682
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 3 cut(s) 43, 305, 711
BstNI CCWGG 2 cut(s) 442, 564
BstSCI CCNGG 2 cut(s) 440, 562
BstUI CGCG 3 cut(s) 348, 694, 712
BstV1I GCAGC 1 cut(s) 424
BstV2I GAAGAC 2 cut(s) 220, 648
BstX2I RGATCY 3 cut(s) 379, 491, 682
BstYI RGATCY 3 cut(s) 379, 491, 682
BsuRI GGCC 2 cut(s) 285, 737
BtsCI GGATG 2 cut(s) 607, 772
BtsIMutI CAGTG 3 cut(s) 129, 469, 714
BveI ACCTGC 1 cut(s) 473
CfoI GCGC 2 cut(s) 350, 714
Cfr13I GGNCC 2 cut(s) 445, 735
CviAII CATG 1 cut(s) 160
DdeI CTNAG 3 cut(s) 47, 269, 338
DpnI GATC 5 cut(s) 345, 366, 381, 493, 684
DpnII GATC 5 cut(s) 343, 364, 379, 491, 682
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 445
Eco88I CYCGRG 2 cut(s) 38, 122
EcoRII CCWGG 2 cut(s) 440, 562
EcoT14I CCWWGG 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 200
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 1 cut(s) 163
FaiI YATR 6 cut(s) 161, 242, 311, 330, 572, 743
FaqI GGGAC 2 cut(s) 105, 458
FatI CATG 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 438
FokI GGATG 2 cut(s) 594, 759
Fsp4HI GCNGC 1 cut(s) 438
FspBI CTAG 3 cut(s) 218, 417, 497
GlaI GCGC 2 cut(s) 349, 713
GluI GCNGC 1 cut(s) 438
GsaI CCCAGC 1 cut(s) 705
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 2 cut(s) 285, 737
HapII CCGG 1 cut(s) 362
HhaI GCGC 2 cut(s) 350, 714
Hin1II CATG 1 cut(s) 163
Hin6I GCGC 2 cut(s) 348, 712
HinP1I GCGC 2 cut(s) 348, 712
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 2 cut(s) 125, 696
HpaII CCGG 1 cut(s) 362
HphI GGTGA 2 cut(s) 497, 543
Hpy166II GTNNAC 4 cut(s) 6, 129, 136, 551
Hpy188I TCNGA 2 cut(s) 29, 265
Hpy188III TCNNGA 3 cut(s) 122, 362, 515
Hpy8I GTNNAC 4 cut(s) 6, 129, 136, 551
Hpy99I CGWCG 1 cut(s) 118
HpyAV CCTTC 2 cut(s) 66, 296
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 671
HpyCH4V TGCA 4 cut(s) 207, 234, 570, 616
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 305, 711
HpyF3I CTNAG 3 cut(s) 47, 269, 338
HpySE526I ACGT 1 cut(s) 671
Hsp92II CATG 1 cut(s) 163
HspAI GCGC 2 cut(s) 348, 712
Kpn2I TCCGGA 1 cut(s) 361
Kzo9I GATC 5 cut(s) 343, 364, 379, 491, 682
LmnI GCTCC 2 cut(s) 43, 463
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 1 cut(s) 403
MaeI CTAG 3 cut(s) 218, 417, 497
MaeII ACGT 1 cut(s) 671
MaeIII GTNAC 2 cut(s) 168, 667
MalI GATC 5 cut(s) 345, 366, 381, 493, 684
MboI GATC 5 cut(s) 343, 364, 379, 491, 682
MboII GAAGA 3 cut(s) 220, 400, 653
MflI RGATCY 3 cut(s) 379, 491, 682
MluCI AATT 3 cut(s) 392, 542, 730
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 7 cut(s) 34, 42, 82, 369, 523, 570, 751
MroI TCCGGA 1 cut(s) 361
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 2 cut(s) 442, 564
Mva1269I GAATGC 1 cut(s) 705
MvaI CCWGG 2 cut(s) 442, 564
MvnI CGCG 3 cut(s) 348, 694, 712
MwoI GCNNNNNNNGC 3 cut(s) 43, 305, 711
NdeII GATC 5 cut(s) 343, 364, 379, 491, 682
NlaIII CATG 1 cut(s) 163
NlaIV GGNNCC 3 cut(s) 54, 446, 493
NmuCI GTSAC 2 cut(s) 168, 667
NspV TTCGAA 1 cut(s) 59
PaqCI CACCTGC 1 cut(s) 473
PctI GAATGC 1 cut(s) 705
PfeI GAWTC 1 cut(s) 696
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 1 cut(s) 439
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
Psp6I CCWGG 2 cut(s) 440, 562
PspFI CCCAGC 1 cut(s) 701
PspGI CCWGG 2 cut(s) 440, 562
PspN4I GGNNCC 3 cut(s) 54, 446, 493
PspPI GGNCC 2 cut(s) 445, 735
PsuI RGATCY 3 cut(s) 379, 491, 682
PsyI GACNNNGTC 1 cut(s) 224
SatI GCNGC 1 cut(s) 438
Sau3AI GATC 5 cut(s) 343, 364, 379, 491, 682
Sau96I GGNCC 2 cut(s) 445, 735
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 2 cut(s) 442, 564
SfaNI GCATC 1 cut(s) 403
SfuI TTCGAA 1 cut(s) 59
SinI GGWCC 1 cut(s) 445
Sse9I AATT 3 cut(s) 392, 542, 730
SsiI CCGC 3 cut(s) 23, 35, 710
SspMI CTAG 3 cut(s) 218, 417, 497
StyD4I CCNGG 2 cut(s) 440, 562
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 674
TaqI TCGA 3 cut(s) 59, 411, 528
TaqII GACCGA 1 cut(s) 462
TasI AATT 3 cut(s) 392, 542, 730
TfiI GAWTC 1 cut(s) 696
TscAI CASTG 3 cut(s) 136, 476, 721
TseFI GTSAC 2 cut(s) 168, 667
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 2 cut(s) 168, 667
TspDTI ATGAA 3 cut(s) 148, 596, 761
TspRI CASTG 3 cut(s) 136, 476, 721
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 445
XapI RAATTY 2 cut(s) 392, 542
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XspI CTAG 3 cut(s) 218, 417, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.