Prupe.4G045300_v2.0.a1

DUF3700

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0017391
Physical Location & Seq
Forward (+)
2167567 .. 2169080
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G045300.1

Sequence Viewer

Length: 813 bp
ATGTTGGCGGTTTTTGAGAAAGCAATTGGAAAACCACCTGAGGAGCTGAGGCTTCCTTCGAATGTGTTGGAGAAGGCAAAGACCAGAGAGGAAATTTTGGAGACTTTCCAATCTTCATGTCCTGAGAGCACGGTGTACTATCTTTCAAATGGGAATTTCATGGCCTTGTCCCATGCCGACGAAAGTATTCAATCACACCCAAGGTCCATAGTTGTCATAGACGATGTCTTCTGCATTTTTATAGGGATTTTGGAGAACACTTGTGACCTTAGAAGGCACTACGGCCTTCCAAGACAAGCAACAGAAGCCATGGTTGTGGTTGAAGCTTATAAAGTTCTAAGAGATCGAGCACCCTACCCGCCAGATCAAGTCATCAAAGATCTGCAAGGAAAATTCGCATTTATTCTCTTCGATGCTAGATTTGGTACCCTTTTTGCAGCGAGGGATCGAGATGGAAGTGTGGAGCTTCAGTGGGCCATGGCAGGTGATGGATCCCTAGTCTACTCTCATGATCTCGACATCATCAGAGAGGCTTGTGGAAAATCTTTTGCACCTTTTCCTCCAGGCTGCATATTTACAAGTGGAAGTGGGCTGACGAGCTTTGTTCATCCCTTACACAAGGTAAGGGCAAGCACACACGAAGATGATGATGGCCATATTTGTGGAGTTACTTTTCAGGTGGATCTGTTCACCCGACTCCCCAGCATCCCGCGCACCGGCAGTGCAGGAAATTGGGCTGATCAAGCACCTGTGGTCAAGGAAGGAGAAGGAAATTGGTCTGATCAAGCAACTGTGGTCAAGGAAGGAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

271

Amino Acids

29.85

Weight (kDa)

5.13

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017391)

Species Orthologous Gene IDs
malus_domestica MD00G1095400.v1.1
prunus_persica Prupe.4G045300_v2.0.a1
pyrus_communis pycom10g24780
rosa_chinensis RchiOBHm_Chr5g0008201
rosa_laevigata RLG00000031546
rosa_multiflora Rmu_sc0001626.1_g000020 Rmu_sc0035773.1_g000001
rosa_rugosa Rorug04G0434200.1
rosa_samantha Rh5AG062300 Rh5BG058800 Rh5CG069300 Rh5DG058200
rosa_wichuraiana Rw5G005840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 330
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 1 cut(s) 473
Acc65I GGTACC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 501
AccII CGCG 1 cut(s) 712
AciI CCGC 3 cut(s) 8, 359, 710
AclWI GGATC 4 cut(s) 453, 486, 499, 690
AcoI YGGCCR 1 cut(s) 652
AcsI RAATTY 3 cut(s) 93, 154, 392
AcuI CTGAAG 1 cut(s) 452
AfaI GTAC 2 cut(s) 137, 427
AfiI CCNNNNNNNGG 1 cut(s) 716
AgsI TTSAA 3 cut(s) 147, 191, 323
AjnI CCWGG 1 cut(s) 562
AluBI AGCT 4 cut(s) 46, 326, 466, 600
AluI AGCT 4 cut(s) 46, 326, 466, 600
Alw21I GWGCWC 2 cut(s) 131, 352
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 4 cut(s) 453, 486, 499, 690
AoxI GGCC 4 cut(s) 162, 283, 474, 652
ApeKI GCWGC 2 cut(s) 437, 567
ApoI RAATTY 3 cut(s) 93, 154, 392
Asp700I GAANNNNTTC 1 cut(s) 186
Asp718I GGTACC 1 cut(s) 425
AspLEI GCGC 1 cut(s) 714
AspS9I GGNCC 2 cut(s) 204, 474
AsuHPI GGTGA 2 cut(s) 497, 682
AsuII TTCGAA 1 cut(s) 59
AvaII GGWCC 1 cut(s) 204
AxyI CCTNAGG 1 cut(s) 39
BalI TGGCCA 1 cut(s) 654
BamHI GGATCC 1 cut(s) 491
BanI GGYRCC 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 2 cut(s) 131, 352
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 2 cut(s) 449, 554
BccI CCATC 3 cut(s) 446, 482, 644
BceAI ACGGC 1 cut(s) 298
BciT130I CCWGG 1 cut(s) 564
BclI TGATCA 2 cut(s) 739, 781
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 2 cut(s) 417, 497
BfuAI ACCTGC 1 cut(s) 473
BglII AGATCT 1 cut(s) 379
BisI GCNGC 2 cut(s) 438, 568
BlsI GCNGC 2 cut(s) 439, 569
Bme1390I CCNGG 1 cut(s) 564
Bme18I GGWCC 1 cut(s) 204
BmgT120I GGNCC 2 cut(s) 204, 474
BmiI GGNNCC 2 cut(s) 427, 493
BmrFI CCNGG 1 cut(s) 564
BmsI GCATC 2 cut(s) 403, 714
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 47
Bpu14I TTCGAA 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 200, 309, 477
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 1 cut(s) 716
Bse118I RCCGGY 1 cut(s) 716
Bse21I CCTNAGG 1 cut(s) 39
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 200, 309, 477
BseGI GGATG 2 cut(s) 607, 705
BseLI CCNNNNNNNGG 1 cut(s) 716
BseMII CTCAG 3 cut(s) 30, 38, 114
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 2 cut(s) 449, 554
BseYI CCCAGC 1 cut(s) 701
BsgI GTGCAG 1 cut(s) 744
Bsh1236I CGCG 1 cut(s) 712
BshFI GGCC 4 cut(s) 164, 285, 476, 654
BshNI GGYRCC 1 cut(s) 425
BsiHKAI GWGCWC 2 cut(s) 131, 352
BsiSI CCGG 1 cut(s) 717
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 1 cut(s) 716
BsmAI GTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 4 cut(s) 164, 285, 476, 654
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 2 cut(s) 131, 352
Bsp143I GATC 9 cut(s) 343, 364, 379, 445, 491, 511, 682, 739, 781
Bsp19I CCATGG 2 cut(s) 309, 477
BspACI CCGC 3 cut(s) 8, 359, 710
BspANI GGCC 4 cut(s) 164, 285, 476, 654
BspCNI CTCAG 3 cut(s) 31, 39, 115
BspFNI CGCG 1 cut(s) 712
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 2 cut(s) 427, 493
BspMI ACCTGC 1 cut(s) 473
BspPI GGATC 4 cut(s) 453, 486, 499, 690
BspT104I TTCGAA 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 425
BsrFI RCCGGY 1 cut(s) 716
BssAI RCCGGY 1 cut(s) 716
BssECI CCNNGG 3 cut(s) 200, 309, 477
BssMI GATC 9 cut(s) 343, 364, 379, 445, 491, 511, 682, 739, 781
BssT1I CCWWGG 3 cut(s) 200, 309, 477
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 2 cut(s) 133, 793
Bst6I CTCTTC 1 cut(s) 413
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 631
BstDEI CTNAG 5 cut(s) 39, 47, 123, 269, 338
BstDSI CCRYGG 2 cut(s) 309, 477
BstF5I GGATG 2 cut(s) 607, 705
BstFNI CGCG 1 cut(s) 712
BstHHI GCGC 1 cut(s) 714
BstKTI GATC 9 cut(s) 346, 367, 382, 448, 494, 514, 685, 742, 784
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 9 cut(s) 343, 364, 379, 445, 491, 511, 682, 739, 781
BstMWI GCNNNNNNNGC 3 cut(s) 305, 711, 743
BstNI CCWGG 1 cut(s) 564
BstSCI CCNGG 1 cut(s) 562
BstUI CGCG 1 cut(s) 712
BstV1I GCAGC 2 cut(s) 449, 554
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 3 cut(s) 379, 491, 682
BstXI CCANNNNNNTGG 2 cut(s) 316, 662
BstYI RGATCY 3 cut(s) 379, 491, 682
Bsu36I CCTNAGG 1 cut(s) 39
BsuRI GGCC 4 cut(s) 164, 285, 476, 654
BtgI CCRYGG 2 cut(s) 309, 477
BtsCI GGATG 2 cut(s) 607, 705
BtsI GCAGTG 1 cut(s) 727
BtsIMutI CAGTG 2 cut(s) 476, 727
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 1 cut(s) 631
CciI TCATGA 1 cut(s) 508
CfoI GCGC 1 cut(s) 714
Cfr10I RCCGGY 1 cut(s) 716
Cfr13I GGNCC 2 cut(s) 204, 474
Csp6I GTAC 2 cut(s) 136, 426
CviAII CATG 6 cut(s) 117, 160, 173, 310, 478, 509
CviQI GTAC 2 cut(s) 136, 426
DdeI CTNAG 5 cut(s) 39, 47, 123, 269, 338
DpnI GATC 9 cut(s) 345, 366, 381, 447, 493, 513, 684, 741, 783
DpnII GATC 9 cut(s) 343, 364, 379, 445, 491, 511, 682, 739, 781
EaeI YGGCCR 1 cut(s) 652
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
Eco130I CCWWGG 3 cut(s) 200, 309, 477
Eco47I GGWCC 1 cut(s) 204
Eco57I CTGAAG 1 cut(s) 452
Eco81I CCTNAGG 1 cut(s) 39
EcoRII CCWGG 1 cut(s) 562
EcoT14I CCWWGG 3 cut(s) 200, 309, 477
ErhI CCWWGG 3 cut(s) 200, 309, 477
FaeI CATG 6 cut(s) 120, 163, 176, 313, 481, 512
FaqI GGGAC 1 cut(s) 154
FatI CATG 6 cut(s) 116, 159, 172, 309, 477, 508
FauI CCCGC 2 cut(s) 366, 717
FbaI TGATCA 2 cut(s) 739, 781
FblI GTMKAC 1 cut(s) 501
Fnu4HI GCNGC 2 cut(s) 438, 568
FokI GGATG 2 cut(s) 594, 692
Fsp4HI GCNGC 2 cut(s) 438, 568
FspBI CTAG 2 cut(s) 417, 497
GlaI GCGC 1 cut(s) 713
GluI GCNGC 2 cut(s) 438, 568
GsaI CCCAGC 1 cut(s) 705
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 4 cut(s) 164, 285, 476, 654
HapII CCGG 1 cut(s) 717
HhaI GCGC 1 cut(s) 714
Hin1II CATG 6 cut(s) 120, 163, 176, 313, 481, 512
Hin6I GCGC 1 cut(s) 712
HinP1I GCGC 1 cut(s) 712
HindIII AAGCTT 1 cut(s) 324
HinfI GANTC 1 cut(s) 696
HpaII CCGG 1 cut(s) 717
HphI GGTGA 2 cut(s) 497, 682
Hpy166II GTNNAC 3 cut(s) 136, 502, 690
Hpy188I TCNGA 2 cut(s) 527, 781
Hpy188III TCNNGA 4 cut(s) 122, 449, 509, 515
Hpy8I GTNNAC 3 cut(s) 136, 502, 690
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 7 cut(s) 66, 67, 267, 296, 755, 761, 797
HpyCH4III ACNGT 2 cut(s) 133, 793
HpyCH4V TGCA 6 cut(s) 234, 385, 437, 551, 570, 725
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 711, 743
HpyF3I CTNAG 5 cut(s) 39, 47, 123, 269, 338
Hsp92II CATG 6 cut(s) 120, 163, 176, 313, 481, 512
HspAI GCGC 1 cut(s) 712
KpnI GGTACC 1 cut(s) 429
Ksp22I TGATCA 2 cut(s) 739, 781
Kzo9I GATC 9 cut(s) 343, 364, 379, 445, 491, 511, 682, 739, 781
LmnI GCTCC 2 cut(s) 43, 463
Lsp1109I GCAGC 2 cut(s) 449, 554
LweI GCATC 2 cut(s) 403, 714
MaeI CTAG 2 cut(s) 417, 497
MaeIII GTNAC 2 cut(s) 263, 667
MalI GATC 9 cut(s) 345, 366, 381, 447, 493, 513, 684, 741, 783
MboI GATC 9 cut(s) 343, 364, 379, 445, 491, 511, 682, 739, 781
MboII GAAGA 4 cut(s) 105, 220, 400, 653
MfeI CAATTG 1 cut(s) 24
MflI RGATCY 3 cut(s) 379, 491, 682
MhlI GDGCHC 2 cut(s) 131, 352
MlsI TGGCCA 1 cut(s) 654
MluCI AATT 6 cut(s) 24, 93, 154, 392, 730, 772
MluNI TGGCCA 1 cut(s) 654
MlyI GAGTC 1 cut(s) 690
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 6 cut(s) 34, 42, 82, 435, 523, 570
Mox20I TGGCCA 1 cut(s) 654
MroXI GAANNNNTTC 1 cut(s) 186
MscI TGGCCA 1 cut(s) 654
MslI CAYNNNNRTG 3 cut(s) 314, 642, 660
Msp20I TGGCCA 1 cut(s) 654
MspI CCGG 1 cut(s) 717
MspR9I CCNGG 1 cut(s) 564
MunI CAATTG 1 cut(s) 24
MvaI CCWGG 1 cut(s) 564
MvnI CGCG 1 cut(s) 712
MwoI GCNNNNNNNGC 3 cut(s) 305, 711, 743
NcoI CCATGG 2 cut(s) 309, 477
NdeII GATC 9 cut(s) 343, 364, 379, 445, 491, 511, 682, 739, 781
NlaIII CATG 6 cut(s) 120, 163, 176, 313, 481, 512
NlaIV GGNNCC 2 cut(s) 427, 493
NmuCI GTSAC 1 cut(s) 263
NspV TTCGAA 1 cut(s) 59
PagI TCATGA 1 cut(s) 508
PaqCI CACCTGC 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 186
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 2 cut(s) 439, 569
PleI GAGTC 1 cut(s) 690
PpsI GAGTC 1 cut(s) 690
PsiI TTATAA 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 562
PspFI CCCAGC 1 cut(s) 701
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 2 cut(s) 427, 493
PspPI GGNCC 2 cut(s) 204, 474
PsuI RGATCY 3 cut(s) 379, 491, 682
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 2 cut(s) 137, 427
RsaNI GTAC 2 cut(s) 136, 426
RseI CAYNNNNRTG 3 cut(s) 314, 642, 660
SatI GCNGC 2 cut(s) 438, 568
Sau3AI GATC 9 cut(s) 343, 364, 379, 445, 491, 511, 682, 739, 781
Sau96I GGNCC 2 cut(s) 204, 474
SchI GAGTC 1 cut(s) 690
ScrFI CCNGG 1 cut(s) 564
SduI GDGCHC 2 cut(s) 131, 352
SfaNI GCATC 2 cut(s) 403, 714
SfuI TTCGAA 1 cut(s) 59
SinI GGWCC 1 cut(s) 204
SmiMI CAYNNNNRTG 3 cut(s) 314, 642, 660
Sse9I AATT 6 cut(s) 24, 93, 154, 392, 730, 772
SsiI CCGC 3 cut(s) 8, 359, 710
SspMI CTAG 2 cut(s) 417, 497
StyD4I CCNGG 1 cut(s) 562
StyI CCWWGG 3 cut(s) 200, 309, 477
TaaI ACNGT 2 cut(s) 133, 793
TaqI TCGA 5 cut(s) 59, 346, 411, 448, 516
TasI AATT 6 cut(s) 24, 93, 154, 392, 730, 772
TatI WGTACW 1 cut(s) 135
TscAI CASTG 2 cut(s) 476, 727
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 2 cut(s) 437, 567
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 3 cut(s) 105, 148, 596
TspRI CASTG 2 cut(s) 476, 727
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 204
XapI RAATTY 3 cut(s) 93, 154, 392
XmiI GTMKAC 1 cut(s) 501
XmnI GAANNNNTTC 1 cut(s) 186
XspI CTAG 2 cut(s) 417, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.