Rh5BG058800

DUF3700

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017391
Physical Location & Seq
Forward (+)
4859866 .. 4861039
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG058800.1

Sequence Viewer

Length: 774 bp
ATGTTGACGGTTTTTGACAAGGCAACAGGAAACCCTCCTGTAGAGCTGGGGCTTCCTTCAATTGGACCGGAGAAGGCAAAGACCAGAGAGGAAATCTTGAAGCTTTTCCAATCATCACGTCCTGAGTGCACTGTATACTACCTTTCAGATGGGAATTTCATGGCTTTGTCTCATGATGACAATGAAAATCAGTCTCACCCAAGGTCAATTGTAGTCATAGACGATGTCTTCTGCATTTTCATTGGGACTTTGGAGAACACTTCTGACCTGAGACGATACTATGGCCTTCCAAGGCAAGCAACAGAAGCCATGGTTGTAGTTGAAGTCTATAAAGTCCTAAGAGATCGAGCACCCTACCCACCTGATCAAGTCATCAAAGATCTGCAGGGAAAATTTGCATTTATCCTCTTCGATGCTAGATCTGGTACCCTTTTTGCAGCCAGGGACCGAGATGGAAGCGTTGAACTTCACTGGGGAATGGCAGGTGATGGGTCTCTAGTCTGCTCCCACAAACTCAACATCATTAGAGAAGCTTGTGGAAAATGTTGTGCGCCTTTCCCTCCAGGCTGCATATTCACAAATGGAAGTGGTTTGATGAGCTTTGTTCATCCACTGCACAAGGTAAGGGCCATTGCACGCGAAGACGACAGTGGCAACATTTGTGGAGTTGCTTTTCAGGTGGATCTGTTCACACGAATCCCCAGCATTCCACGCACCGGTAGTGGACAGAATTGGGCGGATGCAACAGCTGTCAAGGAAGGAGATCACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

257

Amino Acids

28.31

Weight (kDa)

5.81

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 2 - 227 6.7e-86 Aluminium induced protein
GATase_7 PF13537 56 - 169 8.8e-10 Glutamine amidotransferase domain
GATase_6 PF13522 59 - 166 1.1e-06 Glutamine amidotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017391)

Species Orthologous Gene IDs
malus_domestica MD00G1095400.v1.1
prunus_persica Prupe.4G045300_v2.0.a1
pyrus_communis pycom10g24780
rosa_chinensis RchiOBHm_Chr5g0008201
rosa_laevigata RLG00000031546
rosa_multiflora Rmu_sc0001626.1_g000020 Rmu_sc0035773.1_g000001
rosa_rugosa Rorug04G0434200.1
rosa_samantha Rh5AG062300 Rh5BG058800 Rh5CG069300 Rh5DG058200
rosa_wichuraiana Rw5G005840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 1 cut(s) 473
Acc65I GGTACC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 639
AciI CCGC 1 cut(s) 737
AclWI GGATC 1 cut(s) 690
AcsI RAATTY 2 cut(s) 154, 392
AfaI GTAC 1 cut(s) 427
AfiI CCNNNNNNNGG 2 cut(s) 62, 716
AgeI ACCGGT 1 cut(s) 716
AgsI TTSAA 4 cut(s) 60, 100, 323, 464
AjiI CACGTC 1 cut(s) 119
AjnI CCWGG 2 cut(s) 440, 562
AluBI AGCT 5 cut(s) 46, 103, 533, 600, 749
AluI AGCT 5 cut(s) 46, 103, 533, 600, 749
Alw21I GWGCWC 2 cut(s) 131, 352
Alw26I GTCTC 4 cut(s) 174, 198, 265, 498
Alw44I GTGCAC 1 cut(s) 127
AlwI GGATC 1 cut(s) 690
AoxI GGCC 2 cut(s) 283, 627
ApaLI GTGCAC 1 cut(s) 127
ApeKI GCWGC 2 cut(s) 437, 567
ApoI RAATTY 2 cut(s) 154, 392
AsiGI ACCGGT 1 cut(s) 716
Asp700I GAANNNNTTC 1 cut(s) 104
Asp718I GGTACC 1 cut(s) 425
AspLEI GCGC 1 cut(s) 553
AspS9I GGNCC 3 cut(s) 65, 445, 627
AsuHPI GGTGA 2 cut(s) 188, 497
AvaII GGWCC 2 cut(s) 65, 445
BaeGI GKGCMC 1 cut(s) 131
BanI GGYRCC 1 cut(s) 425
BbsI GAAGAC 2 cut(s) 220, 648
Bbv12I GWGCWC 2 cut(s) 131, 352
BbvI GCAGC 2 cut(s) 449, 554
BccI CCATC 3 cut(s) 143, 446, 482
BciT130I CCWGG 2 cut(s) 442, 564
BclI TGATCA 1 cut(s) 364
BcoDI GTCTC 4 cut(s) 174, 198, 265, 498
BfaI CTAG 3 cut(s) 417, 497, 772
BfmI CTRYAG 2 cut(s) 39, 383
BfuAI ACCTGC 1 cut(s) 473
BglII AGATCT 2 cut(s) 379, 419
BisI GCNGC 2 cut(s) 438, 568
BlsI GCNGC 2 cut(s) 439, 569
Bme1390I CCNGG 2 cut(s) 442, 564
Bme18I GGWCC 2 cut(s) 65, 445
BmgBI CACGTC 1 cut(s) 119
BmgT120I GGNCC 3 cut(s) 65, 445, 627
BmiI GGNNCC 2 cut(s) 427, 446
BmrFI CCNGG 2 cut(s) 442, 564
BmrI ACTGGG 1 cut(s) 481
BmsI GCATC 2 cut(s) 403, 730
BmuI ACTGGG 1 cut(s) 481
BpiI GAAGAC 2 cut(s) 220, 648
BpmI CTGGAG 1 cut(s) 546
BsaI GGTCTC 1 cut(s) 498
BsaJI CCNNGG 4 cut(s) 200, 290, 309, 441
BsaWI WCCGGW 2 cut(s) 67, 716
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 716
Bse118I RCCGGY 1 cut(s) 716
Bse1I ACTGG 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 630
BseBI CCWGG 2 cut(s) 442, 564
BseDI CCNNGG 4 cut(s) 200, 290, 309, 441
BseGI GGATG 2 cut(s) 607, 745
BseLI CCNNNNNNNGG 2 cut(s) 62, 716
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 2 cut(s) 114, 260
BseNI ACTGG 1 cut(s) 476
BseSI GKGCMC 1 cut(s) 131
BseXI GCAGC 2 cut(s) 449, 554
BseYI CCCAGC 2 cut(s) 46, 701
BsgI GTGCAG 1 cut(s) 599
Bsh1236I CGCG 1 cut(s) 639
BshFI GGCC 2 cut(s) 285, 629
BshNI GGYRCC 1 cut(s) 425
BshTI ACCGGT 1 cut(s) 716
BsiHKAI GWGCWC 2 cut(s) 131, 352
BsiSI CCGG 2 cut(s) 68, 717
BslFI GGGAC 2 cut(s) 259, 458
BslI CCNNNNNNNGG 2 cut(s) 62, 716
BsmAI GTCTC 4 cut(s) 174, 198, 265, 498
BsmBI CGTCTC 1 cut(s) 265
BsmFI GGGAC 2 cut(s) 259, 458
BsmI GAATGC 1 cut(s) 705
BsnI GGCC 2 cut(s) 285, 629
Bso31I GGTCTC 1 cut(s) 498
Bsp1286I GDGCHC 2 cut(s) 131, 352
Bsp143I GATC 6 cut(s) 343, 364, 379, 419, 682, 763
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 1 cut(s) 737
BspANI GGCC 2 cut(s) 285, 629
BspCNI CTCAG 2 cut(s) 115, 261
BspFNI CGCG 1 cut(s) 639
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 427, 446
BspMAI CTGCAG 1 cut(s) 387
BspMI ACCTGC 1 cut(s) 473
BspPI GGATC 1 cut(s) 690
BspT107I GGYRCC 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 498
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 716
BsrI ACTGG 1 cut(s) 476
BssAI RCCGGY 1 cut(s) 716
BssECI CCNNGG 4 cut(s) 200, 290, 309, 441
BssMI GATC 6 cut(s) 343, 364, 379, 419, 682, 763
BssNAI GTATAC 1 cut(s) 136
BssT1I CCWWGG 3 cut(s) 200, 290, 309
Bst1107I GTATAC 1 cut(s) 136
Bst2UI CCWGG 2 cut(s) 442, 564
Bst4CI ACNGT 3 cut(s) 10, 133, 650
Bst6I CTCTTC 1 cut(s) 413
BstC8I GCNNGC 2 cut(s) 297, 637
BstDEI CTNAG 3 cut(s) 123, 269, 338
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 2 cut(s) 607, 745
BstFNI CGCG 1 cut(s) 639
BstHHI GCGC 1 cut(s) 553
BstKTI GATC 6 cut(s) 346, 367, 382, 422, 685, 766
BstMAI GTCTC 4 cut(s) 174, 198, 265, 498
BstMBI GATC 6 cut(s) 343, 364, 379, 419, 682, 763
BstMWI GCNNNNNNNGC 2 cut(s) 305, 711
BstNI CCWGG 2 cut(s) 442, 564
BstSCI CCNGG 2 cut(s) 440, 562
BstSFI CTRYAG 2 cut(s) 39, 383
BstSLI GKGCMC 1 cut(s) 131
BstUI CGCG 1 cut(s) 639
BstV1I GCAGC 2 cut(s) 449, 554
BstV2I GAAGAC 2 cut(s) 220, 648
BstX2I RGATCY 3 cut(s) 379, 419, 682
BstYI RGATCY 3 cut(s) 379, 419, 682
BstZ17I GTATAC 1 cut(s) 136
BsuRI GGCC 2 cut(s) 285, 629
BtgI CCRYGG 1 cut(s) 309
BtrI CACGTC 1 cut(s) 119
BtsCI GGATG 2 cut(s) 607, 745
BtsI GCAGTG 2 cut(s) 611, 766
BtsIMutI CAGTG 5 cut(s) 129, 469, 611, 655, 766
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 2 cut(s) 297, 637
CciI TCATGA 1 cut(s) 172
CfoI GCGC 1 cut(s) 553
Cfr10I RCCGGY 1 cut(s) 716
Cfr13I GGNCC 3 cut(s) 65, 445, 627
Csp6I GTAC 1 cut(s) 426
CspAI ACCGGT 1 cut(s) 716
CspCI CAANNNNNGTGG 2 cut(s) 643, 678
CviAII CATG 3 cut(s) 160, 173, 310
CviQI GTAC 1 cut(s) 426
DdeI CTNAG 3 cut(s) 123, 269, 338
DpnI GATC 6 cut(s) 345, 366, 381, 421, 684, 765
DpnII GATC 6 cut(s) 343, 364, 379, 419, 682, 763
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
EciI GGCGGA 1 cut(s) 752
Eco130I CCWWGG 3 cut(s) 200, 290, 309
Eco31I GGTCTC 1 cut(s) 498
Eco47I GGWCC 2 cut(s) 65, 445
EcoRII CCWGG 2 cut(s) 440, 562
EcoT14I CCWWGG 3 cut(s) 200, 290, 309
ErhI CCWWGG 3 cut(s) 200, 290, 309
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 3 cut(s) 163, 176, 313
FaiI YATR 8 cut(s) 136, 161, 174, 218, 282, 311, 330, 572
FaqI GGGAC 2 cut(s) 259, 458
FatI CATG 3 cut(s) 159, 172, 309
FbaI TGATCA 1 cut(s) 364
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 438, 568
FokI GGATG 2 cut(s) 594, 752
Fsp4HI GCNGC 2 cut(s) 438, 568
FspBI CTAG 3 cut(s) 417, 497, 772
GlaI GCGC 1 cut(s) 552
GluI GCNGC 2 cut(s) 438, 568
GsaI CCCAGC 2 cut(s) 50, 705
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 2 cut(s) 285, 629
HapII CCGG 2 cut(s) 68, 717
HhaI GCGC 1 cut(s) 553
Hin1II CATG 3 cut(s) 163, 176, 313
Hin6I GCGC 1 cut(s) 551
HinP1I GCGC 1 cut(s) 551
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 101, 531
HinfI GANTC 1 cut(s) 696
HpaII CCGG 2 cut(s) 68, 717
HphI GGTGA 2 cut(s) 188, 497
Hpy166II GTNNAC 5 cut(s) 6, 129, 136, 690, 725
Hpy188I TCNGA 2 cut(s) 148, 265
Hpy188III TCNNGA 3 cut(s) 97, 122, 173
Hpy8I GTNNAC 5 cut(s) 6, 129, 136, 690, 725
HpyAV CCTTC 4 cut(s) 66, 67, 296, 752
HpyCH4III ACNGT 3 cut(s) 10, 133, 650
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 9 cut(s) 129, 234, 385, 398, 437, 570, 616, 635, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 711
HpyF3I CTNAG 3 cut(s) 123, 269, 338
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 3 cut(s) 163, 176, 313
HspAI GCGC 1 cut(s) 551
KpnI GGTACC 1 cut(s) 429
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 6 cut(s) 343, 364, 379, 419, 682, 763
LmnI GCTCC 1 cut(s) 509
Lsp1109I GCAGC 2 cut(s) 449, 554
LweI GCATC 2 cut(s) 403, 730
MaeI CTAG 3 cut(s) 417, 497, 772
MaeII ACGT 1 cut(s) 118
MalI GATC 6 cut(s) 345, 366, 381, 421, 684, 765
MboI GATC 6 cut(s) 343, 364, 379, 419, 682, 763
MboII GAAGA 3 cut(s) 220, 400, 653
MfeI CAATTG 2 cut(s) 60, 207
MflI RGATCY 3 cut(s) 379, 419, 682
MhlI GDGCHC 2 cut(s) 131, 352
MluCI AATT 5 cut(s) 60, 154, 207, 392, 730
MnlI CCTC 4 cut(s) 45, 82, 416, 570
MroXI GAANNNNTTC 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 2 cut(s) 68, 717
MspR9I CCNGG 2 cut(s) 442, 564
MunI CAATTG 2 cut(s) 60, 207
Mva1269I GAATGC 1 cut(s) 705
MvaI CCWGG 2 cut(s) 442, 564
MvnI CGCG 1 cut(s) 639
MwoI GCNNNNNNNGC 2 cut(s) 305, 711
NcoI CCATGG 1 cut(s) 309
NdeII GATC 6 cut(s) 343, 364, 379, 419, 682, 763
NlaIII CATG 3 cut(s) 163, 176, 313
NlaIV GGNNCC 2 cut(s) 427, 446
PagI TCATGA 1 cut(s) 172
PaqCI CACCTGC 1 cut(s) 473
PctI GAATGC 1 cut(s) 705
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 696
PflFI GACNNNGTC 1 cut(s) 224
PinAI ACCGGT 1 cut(s) 716
PkrI GCNGC 2 cut(s) 439, 569
Psp6I CCWGG 2 cut(s) 440, 562
PspFI CCCAGC 2 cut(s) 46, 701
PspGI CCWGG 2 cut(s) 440, 562
PspN4I GGNNCC 2 cut(s) 427, 446
PspPI GGNCC 3 cut(s) 65, 445, 627
PstI CTGCAG 1 cut(s) 387
PsuI RGATCY 3 cut(s) 379, 419, 682
PsyI GACNNNGTC 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 1 cut(s) 427
RsaNI GTAC 1 cut(s) 426
SatI GCNGC 2 cut(s) 438, 568
Sau3AI GATC 6 cut(s) 343, 364, 379, 419, 682, 763
Sau96I GGNCC 3 cut(s) 65, 445, 627
ScrFI CCNGG 2 cut(s) 442, 564
SduI GDGCHC 2 cut(s) 131, 352
SfaNI GCATC 2 cut(s) 403, 730
SfcI CTRYAG 2 cut(s) 39, 383
SinI GGWCC 2 cut(s) 65, 445
Sse9I AATT 5 cut(s) 60, 154, 207, 392, 730
SsiI CCGC 1 cut(s) 737
SspMI CTAG 3 cut(s) 417, 497, 772
StyD4I CCNGG 2 cut(s) 440, 562
StyI CCWWGG 3 cut(s) 200, 290, 309
TaaI ACNGT 3 cut(s) 10, 133, 650
TaiI ACGT 1 cut(s) 121
TaqI TCGA 2 cut(s) 346, 411
TaqII GACCGA 1 cut(s) 462
TasI AATT 5 cut(s) 60, 154, 207, 392, 730
TfiI GAWTC 1 cut(s) 696
TscAI CASTG 5 cut(s) 136, 476, 618, 655, 773
TseI GCWGC 2 cut(s) 437, 567
TspDTI ATGAA 4 cut(s) 148, 198, 229, 596
TspRI CASTG 5 cut(s) 136, 476, 618, 655, 773
Tth111I GACNNNGTC 1 cut(s) 224
VneI GTGCAC 1 cut(s) 127
VpaK11BI GGWCC 2 cut(s) 65, 445
XapI RAATTY 2 cut(s) 154, 392
XmiI GTMKAC 1 cut(s) 135
XmnI GAANNNNTTC 1 cut(s) 104
XspI CTAG 3 cut(s) 417, 497, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.