RchiOBHm_Chr5g0008201

DUF3700

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0017391
Physical Location & Seq
Forward (+)
5243448 .. 5245724
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28918

Sequence Viewer

Length: 771 bp
ATGTTGACGGTTTTCGACAAGGCAACAGGAAACCCTCCTGTAGAGCTGGGGCTTCCTTCGATTGGGCCGGAGAAGGCAAAGACCAGAGAGGAAATCTTGAAGCTTTTCCAATCATCACGTCCTGAGTGCACTATATACTACCTTTCAGATGGGAATTTCATGGCTTTGTCTCATGACAATGAAAATCAGTCTCACCCAAGGTCAATTGTAGTCATAGACGATGTCTTCTGCATTTTCTTTGGGACTTTGGAGAACACTTCTGACCTGAGACGATACTATGGCCTTCCAAGGCAAGCAACAGAAGCCATGGTTGTGGTTGAAGTCTATAAAGTCCTAAGAGATCGAGCACCCTACCCAGCTGATCAAGTCATCAAAGATCTACAGGGAAAATTTGCATTTATCCTCTTCGATGCTAGATCTGGTACCCTTTTTGCAGCCAGGGACCGAGATGCAAGCGTTGAACTTCACTGGGGAATGGCAGGTGATGGGTCTCTAGTCTGCTCCCACAAACTCAACATCATAAGAGAAGCTTGTGGAAAATGTTGTGCACCTTTCCCTCCAGGCTGCATATTCTCAAATGGAAGTGGTTTGATGAGCTTTATTCATCCACTACACAAGGTAAGGGCAATTGCACGCGAAGACGACAGTGGCAACATTTGTGGAGTTTCTTTTCAGGTGGATCTGTTCACGCGAATCCCCAGCATTCCACGCACCTTTAGTGGACAGAATTGGGCGGATGCAACTGCTGTCAAGGAAGGAGATCACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

256

Amino Acids

28.4

Weight (kDa)

5.98

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 2 - 226 1.4e-86 Aluminium induced protein
GATase_6 PF13522 62 - 164 5.3e-06 Glutamine amidotransferase domain
GATase_7 PF13537 73 - 168 2e-08 Glutamine amidotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017391)

Species Orthologous Gene IDs
malus_domestica MD00G1095400.v1.1
prunus_persica Prupe.4G045300_v2.0.a1
pyrus_communis pycom10g24780
rosa_chinensis RchiOBHm_Chr5g0008201
rosa_laevigata RLG00000031546
rosa_multiflora Rmu_sc0001626.1_g000020 Rmu_sc0035773.1_g000001
rosa_rugosa Rorug04G0434200.1
rosa_samantha Rh5AG062300 Rh5BG058800 Rh5CG069300 Rh5DG058200
rosa_wichuraiana Rw5G005840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 470
Acc36I ACCTGC 1 cut(s) 470
Acc65I GGTACC 1 cut(s) 422
AccB1I GGYRCC 1 cut(s) 422
AccII CGCG 2 cut(s) 636, 691
AciI CCGC 1 cut(s) 734
AclWI GGATC 1 cut(s) 687
AcsI RAATTY 2 cut(s) 154, 389
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 1 cut(s) 62
AgsI TTSAA 3 cut(s) 100, 320, 461
AjiI CACGTC 1 cut(s) 119
AjnI CCWGG 2 cut(s) 437, 559
AluBI AGCT 5 cut(s) 46, 103, 359, 530, 597
AluI AGCT 5 cut(s) 46, 103, 359, 530, 597
Alw21I GWGCWC 3 cut(s) 131, 349, 550
Alw26I GTCTC 4 cut(s) 174, 195, 262, 495
Alw44I GTGCAC 2 cut(s) 127, 546
AlwI GGATC 1 cut(s) 687
AoxI GGCC 2 cut(s) 65, 280
ApaLI GTGCAC 2 cut(s) 127, 546
ApeKI GCWGC 2 cut(s) 434, 564
ApoI RAATTY 2 cut(s) 154, 389
Asp700I GAANNNNTTC 1 cut(s) 104
Asp718I GGTACC 1 cut(s) 422
AspS9I GGNCC 2 cut(s) 65, 442
AsuHPI GGTGA 2 cut(s) 185, 494
AvaII GGWCC 1 cut(s) 442
BaeGI GKGCMC 2 cut(s) 131, 550
BanI GGYRCC 1 cut(s) 422
BbsI GAAGAC 2 cut(s) 217, 645
Bbv12I GWGCWC 3 cut(s) 131, 349, 550
BbvI GCAGC 2 cut(s) 446, 551
BccI CCATC 2 cut(s) 143, 479
BciT130I CCWGG 2 cut(s) 439, 561
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 4 cut(s) 174, 195, 262, 495
BfaI CTAG 3 cut(s) 414, 494, 769
BfmI CTRYAG 2 cut(s) 39, 380
BfuAI ACCTGC 1 cut(s) 470
BglII AGATCT 2 cut(s) 376, 416
BisI GCNGC 2 cut(s) 435, 565
BlsI GCNGC 2 cut(s) 436, 566
Bme1390I CCNGG 2 cut(s) 439, 561
Bme18I GGWCC 1 cut(s) 442
BmgBI CACGTC 1 cut(s) 119
BmgT120I GGNCC 2 cut(s) 65, 442
BmiI GGNNCC 2 cut(s) 424, 443
BmrFI CCNGG 2 cut(s) 439, 561
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 3 cut(s) 400, 439, 727
BmuI ACTGGG 1 cut(s) 478
BpiI GAAGAC 2 cut(s) 217, 645
BpmI CTGGAG 1 cut(s) 543
BsaI GGTCTC 1 cut(s) 495
BsaJI CCNNGG 4 cut(s) 197, 287, 306, 438
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 473
BseBI CCWGG 2 cut(s) 439, 561
BseDI CCNNGG 4 cut(s) 197, 287, 306, 438
BseGI GGATG 2 cut(s) 604, 742
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 2 cut(s) 114, 257
BseNI ACTGG 1 cut(s) 473
BseSI GKGCMC 2 cut(s) 131, 550
BseXI GCAGC 2 cut(s) 446, 551
BseYI CCCAGC 3 cut(s) 46, 355, 698
Bsh1236I CGCG 2 cut(s) 636, 691
BshFI GGCC 2 cut(s) 67, 282
BshNI GGYRCC 1 cut(s) 422
BsiHKAI GWGCWC 3 cut(s) 131, 349, 550
BsiSI CCGG 1 cut(s) 68
BslFI GGGAC 2 cut(s) 256, 455
BslI CCNNNNNNNGG 1 cut(s) 62
BsmAI GTCTC 4 cut(s) 174, 195, 262, 495
BsmBI CGTCTC 1 cut(s) 262
BsmFI GGGAC 2 cut(s) 256, 455
BsmI GAATGC 1 cut(s) 702
BsnI GGCC 2 cut(s) 67, 282
Bso31I GGTCTC 1 cut(s) 495
Bsp1286I GDGCHC 3 cut(s) 131, 349, 550
Bsp143I GATC 6 cut(s) 340, 361, 376, 416, 679, 760
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 1 cut(s) 734
BspANI GGCC 2 cut(s) 67, 282
BspCNI CTCAG 2 cut(s) 115, 258
BspFNI CGCG 2 cut(s) 636, 691
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 424, 443
BspMI ACCTGC 1 cut(s) 470
BspPI GGATC 1 cut(s) 687
BspT107I GGYRCC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 495
BsrI ACTGG 1 cut(s) 473
BssECI CCNNGG 4 cut(s) 197, 287, 306, 438
BssMI GATC 6 cut(s) 340, 361, 376, 416, 679, 760
BssT1I CCWWGG 3 cut(s) 197, 287, 306
Bst2UI CCWGG 2 cut(s) 439, 561
Bst4CI ACNGT 2 cut(s) 10, 647
Bst6I CTCTTC 1 cut(s) 410
BstC8I GCNNGC 3 cut(s) 294, 454, 634
BstDEI CTNAG 3 cut(s) 123, 266, 335
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 604, 742
BstFNI CGCG 2 cut(s) 636, 691
BstKTI GATC 6 cut(s) 343, 364, 379, 419, 682, 763
BstMAI GTCTC 4 cut(s) 174, 195, 262, 495
BstMBI GATC 6 cut(s) 340, 361, 376, 416, 679, 760
BstMWI GCNNNNNNNGC 2 cut(s) 302, 708
BstNI CCWGG 2 cut(s) 439, 561
BstSCI CCNGG 2 cut(s) 437, 559
BstSFI CTRYAG 2 cut(s) 39, 380
BstSLI GKGCMC 2 cut(s) 131, 550
BstUI CGCG 2 cut(s) 636, 691
BstV1I GCAGC 2 cut(s) 446, 551
BstV2I GAAGAC 2 cut(s) 217, 645
BstX2I RGATCY 3 cut(s) 376, 416, 679
BstXI CCANNNNNNTGG 1 cut(s) 313
BstYI RGATCY 3 cut(s) 376, 416, 679
BsuRI GGCC 2 cut(s) 67, 282
BtgI CCRYGG 1 cut(s) 306
BtrI CACGTC 1 cut(s) 119
BtsCI GGATG 2 cut(s) 604, 742
BtsIMutI CAGTG 2 cut(s) 466, 652
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 3 cut(s) 294, 454, 634
CciI TCATGA 1 cut(s) 172
Cfr13I GGNCC 2 cut(s) 65, 442
Csp6I GTAC 1 cut(s) 423
CspCI CAANNNNNGTGG 2 cut(s) 640, 675
CviAII CATG 3 cut(s) 160, 173, 307
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 3 cut(s) 123, 266, 335
DpnI GATC 6 cut(s) 342, 363, 378, 418, 681, 762
DpnII GATC 6 cut(s) 340, 361, 376, 416, 679, 760
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
EciI GGCGGA 1 cut(s) 749
Eco130I CCWWGG 3 cut(s) 197, 287, 306
Eco31I GGTCTC 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 442
EcoRII CCWGG 2 cut(s) 437, 559
EcoT14I CCWWGG 3 cut(s) 197, 287, 306
ErhI CCWWGG 3 cut(s) 197, 287, 306
Esp3I CGTCTC 1 cut(s) 262
FaeI CATG 3 cut(s) 163, 176, 310
FalI AAGNNNNNCTT 2 cut(s) 514, 546
FaqI GGGAC 2 cut(s) 256, 455
FatI CATG 3 cut(s) 159, 172, 306
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 2 cut(s) 435, 565
FokI GGATG 2 cut(s) 591, 749
Fsp4HI GCNGC 2 cut(s) 435, 565
FspBI CTAG 3 cut(s) 414, 494, 769
GluI GCNGC 2 cut(s) 435, 565
GsaI CCCAGC 3 cut(s) 50, 359, 702
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 2 cut(s) 67, 282
HapII CCGG 1 cut(s) 68
Hin1II CATG 3 cut(s) 163, 176, 310
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 101, 528
HinfI GANTC 1 cut(s) 693
HpaII CCGG 1 cut(s) 68
HphI GGTGA 2 cut(s) 185, 494
Hpy166II GTNNAC 5 cut(s) 6, 129, 548, 687, 722
Hpy188I TCNGA 2 cut(s) 148, 262
Hpy188III TCNNGA 3 cut(s) 97, 122, 173
Hpy8I GTNNAC 5 cut(s) 6, 129, 548, 687, 722
HpyAV CCTTC 4 cut(s) 66, 67, 293, 749
HpyCH4III ACNGT 2 cut(s) 10, 647
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 9 cut(s) 129, 231, 395, 434, 452, 548, 567, 632, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 708
HpyF3I CTNAG 3 cut(s) 123, 266, 335
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 3 cut(s) 163, 176, 310
KpnI GGTACC 1 cut(s) 426
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 6 cut(s) 340, 361, 376, 416, 679, 760
LmnI GCTCC 1 cut(s) 506
Lsp1109I GCAGC 2 cut(s) 446, 551
LweI GCATC 3 cut(s) 400, 439, 727
MaeI CTAG 3 cut(s) 414, 494, 769
MaeII ACGT 1 cut(s) 118
MalI GATC 6 cut(s) 342, 363, 378, 418, 681, 762
MboI GATC 6 cut(s) 340, 361, 376, 416, 679, 760
MboII GAAGA 3 cut(s) 217, 397, 650
MfeI CAATTG 2 cut(s) 204, 627
MflI RGATCY 3 cut(s) 376, 416, 679
MhlI GDGCHC 3 cut(s) 131, 349, 550
MluCI AATT 5 cut(s) 154, 204, 389, 627, 727
MnlI CCTC 4 cut(s) 45, 82, 413, 567
MroXI GAANNNNTTC 1 cut(s) 104
MslI CAYNNNNRTG 2 cut(s) 177, 311
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 2 cut(s) 439, 561
MunI CAATTG 2 cut(s) 204, 627
Mva1269I GAATGC 1 cut(s) 702
MvaI CCWGG 2 cut(s) 439, 561
MvnI CGCG 2 cut(s) 636, 691
MwoI GCNNNNNNNGC 2 cut(s) 302, 708
NcoI CCATGG 1 cut(s) 306
NdeII GATC 6 cut(s) 340, 361, 376, 416, 679, 760
NlaIII CATG 3 cut(s) 163, 176, 310
NlaIV GGNNCC 2 cut(s) 424, 443
PagI TCATGA 1 cut(s) 172
PaqCI CACCTGC 1 cut(s) 470
PctI GAATGC 1 cut(s) 702
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 693
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 2 cut(s) 436, 566
Psp6I CCWGG 2 cut(s) 437, 559
PspFI CCCAGC 3 cut(s) 46, 355, 698
PspGI CCWGG 2 cut(s) 437, 559
PspN4I GGNNCC 2 cut(s) 424, 443
PspPI GGNCC 2 cut(s) 65, 442
PsuI RGATCY 3 cut(s) 376, 416, 679
PsyI GACNNNGTC 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
RseI CAYNNNNRTG 2 cut(s) 177, 311
SatI GCNGC 2 cut(s) 435, 565
Sau3AI GATC 6 cut(s) 340, 361, 376, 416, 679, 760
Sau96I GGNCC 2 cut(s) 65, 442
ScrFI CCNGG 2 cut(s) 439, 561
SduI GDGCHC 3 cut(s) 131, 349, 550
SfaNI GCATC 3 cut(s) 400, 439, 727
SfcI CTRYAG 2 cut(s) 39, 380
SinI GGWCC 1 cut(s) 442
SmiMI CAYNNNNRTG 2 cut(s) 177, 311
Sse9I AATT 5 cut(s) 154, 204, 389, 627, 727
SsiI CCGC 1 cut(s) 734
SspMI CTAG 3 cut(s) 414, 494, 769
StyD4I CCNGG 2 cut(s) 437, 559
StyI CCWWGG 3 cut(s) 197, 287, 306
TaaI ACNGT 2 cut(s) 10, 647
TaiI ACGT 1 cut(s) 121
TaqI TCGA 4 cut(s) 15, 59, 343, 408
TaqII GACCGA 1 cut(s) 459
TasI AATT 5 cut(s) 154, 204, 389, 627, 727
TfiI GAWTC 1 cut(s) 693
TscAI CASTG 2 cut(s) 473, 652
TseI GCWGC 2 cut(s) 434, 564
TspDTI ATGAA 3 cut(s) 148, 195, 593
TspRI CASTG 2 cut(s) 473, 652
Tth111I GACNNNGTC 1 cut(s) 221
VneI GTGCAC 2 cut(s) 127, 546
VpaK11BI GGWCC 1 cut(s) 442
XapI RAATTY 2 cut(s) 154, 389
XmnI GAANNNNTTC 1 cut(s) 104
XspI CTAG 3 cut(s) 414, 494, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.