Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0015116

Physical Location & Seq

Genomic Coordinates Forward (+)

23561286 .. 23572479

Gene Structure 11194 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1111700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGTCGTTGTACGACGCGGCGTTTGTGAACTCGCAGCTCTCGAAACCGACATCAATCTTCGGGCTCCGTCTCTGGGTCGTCATTGGAATCCTTGTCGGTTCTCTGATAGTCCTGATCCTCTTCCTCCTCTCCCTCTGCCTCACCTCCCGTCATCGCTCCCGGGCCCACAAGGCCAATCCCAACCCCACCGCCGCCGACTTGCCCCCTACTCCAGTCAAATCCAAGGAAATCCAGGAAATCGTCAACGACCTCCACGTCGAGATCGGGAAGCCGGAGCACCGGGTGGTGGTGTATTCGGACCGGGCGTCGAGCGGGGAGAGCAGGGGGACATTGAATAGCGGGTGCGAGACGGCGTCGTTTGGGAGCGGGAATGTGGTGGGGCCGGAGGTGTCGCATCTGGGTTGGGGGAGATGGTACACTCTGAGGGAGCTGGAGGCGGCGACGAATGGGTTGTGTGAGGAGAATGTGATTGGGGAAGGAGGGTATGGGATTGTTTACAGTGGGATTCTCAGTGACGGGAGTAAAGTTGCTGTCAAGAACTTGTTGAATAACAGGGGTCAAGCAGAGAAGGAATTTAAAGTGGAGGTGGAAGCAATTGGGCGTGTGAGACACAAAAATCTTGTAAGATTACTTGGATACTGTGTTGAGGGTGCATACAGGATGCTTGTGTATGAGTACGTTGACAATGGCAATCTGGATCAGTGGCTTCATGGGGATGTTGGTGAAGTCAGCCCGCTAACATGGGATATCCGTATGAATATTATACTGGGAACAGCAAAAGGTTTGGCCTATCTTCATGAGGGTCTTGAACCAAAGGTTGTTCATCGAGATGTGAAATCTAGCAACATACTAATTGATCGCCAGTGGAACCCCAAAGTATCTGATTTTGGGCTTGCTAAGCTCCTGTGCTCCGAGAACAGTTATGTGACAACTCGAGTGATGGGAACATTTGGTTATGTTGCACCGGAATATGCTTGCACGGGAATGCTGAACGAGAAGAGTGATGTTTATAGCTTTGGAATACTTATTATGGAGCTTATATCCGGGAGAAGCCCCGTTGATTATAGTCGACCACAAGGAGAGGTGAATCTAGTTGAATGGTTAAAAACCATGGTTGGGAAACGAAAAGCAGAGGATGTGGTTGATCCCAAGCTGCCCGAGAAGCCTGCTTCAAAAGCACTTAAACGTGCTCTGTTGGTTGCTCTTCGATGTGTTGATCCTGATGCCACAAAAAGGCCAAAAATGGGACATGTGATCCACATGCTCGAGGCTGATGACTTGCTATTCCGTGATGAACGCCAGCCTGGTAGAGATGCGTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

441

Amino Acids

48.61

Weight (kDa)

6.84

Isoelectric Point (pI)

31.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	154 - 419	1e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	154 - 421	2.8e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015116)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01540 AT1G01540 AT4G01330 AT4G01330 AT4G01330
fragaria_vesca	FvH4_7g15080
malus_domestica	MD00G1111700.v1.1 MD01G1069300.v1.1
prunus_persica	Prupe.2G175400_v2.0.a1
pyrus_communis	pycom01g09870
rosa_chinensis	RchiOBHm_Chr1g0357191
rosa_laevigata	RLG00000028045
rosa_multiflora	Rmu_sc0005967.1_g000006
rosa_rugosa	Rorug01G0254800
rosa_samantha	Rh1BG236900
rosa_wichuraiana	Rw1G023740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	254
AccBSI	CCGCTC	2 cut(s)	312, 366
AccI	GTMKAC	1 cut(s)	1069
AccII	CGCG	1 cut(s)	17
AciI	CCGC	8 cut(s)	17, 189, 192, 312, 339, 366, 437, 734
AclWI	GGATC	5 cut(s)	109, 705, 1139, 1211, 1249
AcsI	RAATTY	1 cut(s)	572
AcyI	GRCGYC	2 cut(s)	305, 353
AdeI	CACNNNGTG	1 cut(s)	283
AfaI	GTAC	3 cut(s)	11, 416, 677
AfiI	CCNNNNNNNGG	5 cut(s)	73, 286, 1116, 1233, 1244
AflIII	ACRYGT	1 cut(s)	1249
AgsI	TTSAA	5 cut(s)	334, 547, 809, 1097, 1173
AhdI	GACNNNNNGTC	1 cut(s)	304
AjiI	CACGTC	1 cut(s)	256
AjnI	CCWGG	2 cut(s)	231, 1303
AluBI	AGCT	6 cut(s)	37, 430, 901, 1014, 1036, 1153
AluI	AGCT	6 cut(s)	37, 430, 901, 1014, 1036, 1153
Alw21I	GWGCWC	3 cut(s)	279, 911, 1192
Alw26I	GTCTC	3 cut(s)	74, 341, 601
AlwI	GGATC	5 cut(s)	109, 705, 1139, 1211, 1249
Ama87I	CYCGRG	4 cut(s)	159, 933, 1157, 1265
AoxI	GGCC	5 cut(s)	162, 171, 380, 786, 1235
ApaI	GGGCCC	1 cut(s)	166
ApeKI	GCWGC	2 cut(s)	34, 1153
ApoI	RAATTY	1 cut(s)	572
ArsI	GACNNNNNNTTYG	1 cut(s)	37
AspS9I	GGNCC	4 cut(s)	162, 163, 298, 380
AsuC2I	CCSGG	5 cut(s)	160, 161, 281, 302, 1045
AsuHPI	GGTGA	3 cut(s)	133, 734, 1096
AvaI	CYCGRG	4 cut(s)	159, 933, 1157, 1265
AvaII	GGWCC	1 cut(s)	298
BaeGI	GKGCMC	1 cut(s)	166
BanII	GRGCYC	2 cut(s)	66, 166
Bbv12I	GWGCWC	3 cut(s)	279, 911, 1192
BbvI	GCAGC	2 cut(s)	46, 1140
BccI	CCATC	2 cut(s)	405, 934
BceAI	ACGGC	1 cut(s)	366
BcgI	CGANNNNNNTGC	2 cut(s)	1148, 1182
BciT130I	CCWGG	2 cut(s)	233, 1305
BciVI	GTATCC	1 cut(s)	629
BcnI	CCSGG	5 cut(s)	160, 161, 281, 302, 1045
BcoDI	GTCTC	3 cut(s)	74, 341, 601
BfaI	CTAG	2 cut(s)	840, 1091
BfuI	GTATCC	1 cut(s)	629
BglI	GCCNNNNNGGC	1 cut(s)	170
BisI	GCNGC	5 cut(s)	18, 35, 192, 438, 1154
BlpI	GCTNAGC	1 cut(s)	897
BlsI	GCNGC	5 cut(s)	19, 36, 193, 439, 1155
Bme1390I	CCNGG	7 cut(s)	160, 161, 233, 281, 302, 1045, 1305
Bme18I	GGWCC	1 cut(s)	298
BmeRI	GACNNNNNGTC	1 cut(s)	304
BmeT110I	CYCGRG	4 cut(s)	159, 933, 1157, 1265
BmgBI	CACGTC	1 cut(s)	256
BmgT120I	GGNCC	4 cut(s)	162, 163, 298, 380
BmiI	GGNNCC	4 cut(s)	65, 164, 381, 869
BmrFI	CCNGG	7 cut(s)	160, 161, 233, 281, 302, 1045, 1305
BmrI	ACTGGG	1 cut(s)	776
BmsI	GCATC	4 cut(s)	403, 651, 1213, 1303
BmuI	ACTGGG	1 cut(s)	776
BpmI	CTGGAG	2 cut(s)	195, 452
Bpu1102I	GCTNAGC	1 cut(s)	897
BpuMI	CCSGG	5 cut(s)	160, 161, 281, 302, 1045
BsaHI	GRCGYC	2 cut(s)	305, 353
BsaJI	CCNNGG	3 cut(s)	159, 222, 1110
BsaWI	WCCGGW	1 cut(s)	964
Bsc4I	CCNNNNNNNGG	5 cut(s)	73, 286, 1116, 1233, 1244
Bse1I	ACTGG	3 cut(s)	212, 771, 862
BseBI	CCWGG	2 cut(s)	233, 1305
BseDI	CCNNGG	3 cut(s)	159, 222, 1110
BseGI	GGATG	3 cut(s)	666, 721, 1141
BseLI	CCNNNNNNNGG	5 cut(s)	73, 286, 1116, 1233, 1244
BseMII	CTCAG	2 cut(s)	413, 523
BseNI	ACTGG	3 cut(s)	212, 771, 862
BseRI	GAGGAG	2 cut(s)	116, 473
BseSI	GKGCMC	1 cut(s)	166
BseXI	GCAGC	2 cut(s)	46, 1140
Bsh1236I	CGCG	1 cut(s)	17
BshFI	GGCC	5 cut(s)	164, 173, 382, 788, 1237
BsiHKAI	GWGCWC	3 cut(s)	279, 911, 1192
BsiHKCI	CYCGRG	4 cut(s)	159, 933, 1157, 1265
BsiSI	CCGG	7 cut(s)	160, 272, 280, 301, 383, 965, 1044
BslFI	GGGAC	2 cut(s)	340, 1260
BslI	CCNNNNNNNGG	5 cut(s)	73, 286, 1116, 1233, 1244
BsmAI	GTCTC	3 cut(s)	74, 341, 601
BsmBI	CGTCTC	2 cut(s)	74, 341
BsmFI	GGGAC	2 cut(s)	340, 1260
BsmI	GAATGC	1 cut(s)	990
BsnI	GGCC	5 cut(s)	164, 173, 382, 788, 1237
BsoBI	CYCGRG	4 cut(s)	159, 933, 1157, 1265
Bsp120I	GGGCCC	1 cut(s)	162
Bsp1286I	GDGCHC	5 cut(s)	66, 166, 279, 911, 1192
Bsp143I	GATC	7 cut(s)	114, 261, 697, 856, 1144, 1216, 1254
Bsp1720I	GCTNAGC	1 cut(s)	897
Bsp19I	CCATGG	1 cut(s)	1110
BspACI	CCGC	8 cut(s)	17, 189, 192, 312, 339, 366, 437, 734
BspANI	GGCC	5 cut(s)	164, 173, 382, 788, 1237
BspCNI	CTCAG	2 cut(s)	414, 522
BspFNI	CGCG	1 cut(s)	17
BspHI	TCATGA	1 cut(s)	796
BspLI	GGNNCC	4 cut(s)	65, 164, 381, 869
BspPI	GGATC	5 cut(s)	109, 705, 1139, 1211, 1249
BspQI	GCTCTTC	1 cut(s)	1209
BsrBI	CCGCTC	2 cut(s)	312, 366
BsrI	ACTGG	3 cut(s)	212, 771, 862
BssECI	CCNNGG	3 cut(s)	159, 222, 1110
BssMI	GATC	7 cut(s)	114, 261, 697, 856, 1144, 1216, 1254
BssNI	GRCGYC	2 cut(s)	305, 353
BssT1I	CCWWGG	2 cut(s)	222, 1110
Bst2UI	CCWGG	2 cut(s)	233, 1305
Bst4CI	ACNGT	3 cut(s)	500, 641, 920
Bst6I	CTCTTC	3 cut(s)	125, 992, 1209
BstACI	GRCGYC	2 cut(s)	305, 353
BstC8I	GCNNGC	5 cut(s)	734, 894, 976, 1167, 1301
BstDEI	CTNAG	3 cut(s)	422, 509, 897
BstDSI	CCRYGG	1 cut(s)	1110
BstF5I	GGATG	3 cut(s)	666, 721, 1141
BstFNI	CGCG	1 cut(s)	17
BstKTI	GATC	7 cut(s)	117, 264, 700, 859, 1147, 1219, 1257
BstMAI	GTCTC	3 cut(s)	74, 341, 601
BstMBI	GATC	7 cut(s)	114, 261, 697, 856, 1144, 1216, 1254
BstMWI	GCNNNNNNNGC	5 cut(s)	170, 318, 898, 1162, 1175
BstNI	CCWGG	2 cut(s)	233, 1305
BstNSI	RCATGY	2 cut(s)	1253, 1264
BstSCI	CCNGG	7 cut(s)	158, 159, 231, 279, 300, 1043, 1303
BstSLI	GKGCMC	1 cut(s)	166
BstUI	CGCG	1 cut(s)	17
BstV1I	GCAGC	2 cut(s)	46, 1140
BsuI	GTATCC	1 cut(s)	629
BsuRI	GGCC	5 cut(s)	164, 173, 382, 788, 1237
BtgI	CCRYGG	1 cut(s)	1110
BtgZI	GCGATG	1 cut(s)	137
BtrI	CACGTC	1 cut(s)	256
BtsCI	GGATG	3 cut(s)	666, 721, 1141
BtsIMutI	CAGTG	4 cut(s)	505, 517, 707, 869
Cac8I	GCNNGC	5 cut(s)	734, 894, 976, 1167, 1301
CciI	TCATGA	1 cut(s)	796
Cfr13I	GGNCC	4 cut(s)	162, 163, 298, 380
Cfr9I	CCCGGG	1 cut(s)	159
CpoI	CGGWCCG	1 cut(s)	298
CseI	GACGC	4 cut(s)	23, 294, 342, 1305
Csp6I	GTAC	3 cut(s)	10, 415, 676
CspI	CGGWCCG	1 cut(s)	298
CviAII	CATG	6 cut(s)	710, 741, 797, 1111, 1250, 1261
CviQI	GTAC	3 cut(s)	10, 415, 676
DdeI	CTNAG	3 cut(s)	422, 509, 897
DpnI	GATC	7 cut(s)	116, 263, 699, 858, 1146, 1218, 1256
DpnII	GATC	7 cut(s)	114, 261, 697, 856, 1144, 1216, 1254
DraI	TTTAAA	1 cut(s)	577
DraIII	CACNNNGTG	1 cut(s)	283
DrdI	GACNNNNNNGTC	1 cut(s)	254
DriI	GACNNNNNGTC	1 cut(s)	304
DseDI	GACNNNNNNGTC	1 cut(s)	254
Eam1104I	CTCTTC	3 cut(s)	125, 992, 1209
Eam1105I	GACNNNNNGTC	1 cut(s)	304
EarI	CTCTTC	3 cut(s)	125, 992, 1209
Eco130I	CCWWGG	2 cut(s)	222, 1110
Eco24I	GRGCYC	2 cut(s)	66, 166
Eco32I	GATATC	1 cut(s)	748
Eco47I	GGWCC	1 cut(s)	298
Eco88I	CYCGRG	4 cut(s)	159, 933, 1157, 1265
EcoRII	CCWGG	2 cut(s)	231, 1303
EcoRV	GATATC	1 cut(s)	748
EcoT14I	CCWWGG	2 cut(s)	222, 1110
EcoT38I	GRGCYC	2 cut(s)	66, 166
ErhI	CCWWGG	2 cut(s)	222, 1110
Esp3I	CGTCTC	2 cut(s)	74, 341
FaeI	CATG	6 cut(s)	713, 744, 800, 1114, 1253, 1264
FaqI	GGGAC	2 cut(s)	340, 1260
FatI	CATG	6 cut(s)	709, 740, 796, 1110, 1249, 1260
FauI	CCCGC	4 cut(s)	305, 332, 359, 741
FblI	GTMKAC	1 cut(s)	1069
Fnu4HI	GCNGC	5 cut(s)	18, 35, 192, 438, 1154
FokI	GGATG	3 cut(s)	673, 728, 1148
FriOI	GRGCYC	2 cut(s)	66, 166
Fsp4HI	GCNGC	5 cut(s)	18, 35, 192, 438, 1154
FspBI	CTAG	2 cut(s)	840, 1091
GluI	GCNGC	5 cut(s)	18, 35, 192, 438, 1154
GsuI	CTGGAG	2 cut(s)	195, 452
HaeIII	GGCC	5 cut(s)	164, 173, 382, 788, 1237
HapII	CCGG	7 cut(s)	160, 272, 280, 301, 383, 965, 1044
HgaI	GACGC	4 cut(s)	23, 294, 342, 1305
Hin1I	GRCGYC	2 cut(s)	305, 353
Hin1II	CATG	6 cut(s)	713, 744, 800, 1114, 1253, 1264
HincII	GTYRAC	3 cut(s)	244, 682, 1070
HindII	GTYRAC	3 cut(s)	244, 682, 1070
HinfI	GANTC	3 cut(s)	87, 505, 1087
HpaII	CCGG	7 cut(s)	160, 272, 280, 301, 383, 965, 1044
HphI	GGTGA	3 cut(s)	133, 734, 1096
Hpy166II	GTNNAC	6 cut(s)	28, 244, 417, 496, 682, 1070
Hpy188I	TCNGA	5 cut(s)	105, 298, 423, 883, 913
Hpy8I	GTNNAC	6 cut(s)	28, 244, 417, 496, 682, 1070
Hpy99I	CGWCG	5 cut(s)	17, 260, 310, 358, 445
HpyAV	CCTTC	2 cut(s)	470, 562
HpyCH4III	ACNGT	3 cut(s)	500, 641, 920
HpyCH4IV	ACGT	3 cut(s)	255, 678, 1186
HpyCH4V	TGCA	3 cut(s)	653, 962, 978
HpyF10VI	GCNNNNNNNGC	5 cut(s)	170, 318, 898, 1162, 1175
HpyF3I	CTNAG	3 cut(s)	422, 509, 897
HpySE526I	ACGT	3 cut(s)	255, 678, 1186
Hsp92I	GRCGYC	2 cut(s)	305, 353
Hsp92II	CATG	6 cut(s)	713, 744, 800, 1114, 1253, 1264
Kzo9I	GATC	7 cut(s)	114, 261, 697, 856, 1144, 1216, 1254
LguI	GCTCTTC	1 cut(s)	1209
LmnI	GCTCC	8 cut(s)	69, 161, 274, 363, 427, 906, 914, 1033
Lsp1109I	GCAGC	2 cut(s)	46, 1140
LweI	GCATC	4 cut(s)	403, 651, 1213, 1303
MaeI	CTAG	2 cut(s)	840, 1091
MaeII	ACGT	3 cut(s)	255, 678, 1186
MaeIII	GTNAC	2 cut(s)	512, 925
MalI	GATC	7 cut(s)	116, 263, 699, 858, 1146, 1218, 1256
MbiI	CCGCTC	2 cut(s)	312, 366
MboI	GATC	7 cut(s)	114, 261, 697, 856, 1144, 1216, 1254
MboII	GAAGA	5 cut(s)	49, 112, 785, 1009, 1196
MfeI	CAATTG	1 cut(s)	594
MhlI	GDGCHC	5 cut(s)	66, 166, 279, 911, 1192
MluCI	AATT	3 cut(s)	572, 594, 852
MseI	TTAA	3 cut(s)	576, 1103, 1182
MslI	CAYNNNNRTG	3 cut(s)	714, 828, 983
MspI	CCGG	7 cut(s)	160, 272, 280, 301, 383, 965, 1044
MspR9I	CCNGG	7 cut(s)	160, 161, 233, 281, 302, 1045, 1305
MunI	CAATTG	1 cut(s)	594
Mva1269I	GAATGC	1 cut(s)	990
MvaI	CCWGG	2 cut(s)	233, 1305
MvnI	CGCG	1 cut(s)	17
MwoI	GCNNNNNNNGC	5 cut(s)	170, 318, 898, 1162, 1175
NciI	CCSGG	5 cut(s)	160, 161, 281, 302, 1045
NcoI	CCATGG	1 cut(s)	1110
NdeII	GATC	7 cut(s)	114, 261, 697, 856, 1144, 1216, 1254
NlaIII	CATG	6 cut(s)	713, 744, 800, 1114, 1253, 1264
NlaIV	GGNNCC	4 cut(s)	65, 164, 381, 869
NmuCI	GTSAC	2 cut(s)	512, 925
NspI	RCATGY	2 cut(s)	1253, 1264
PaeR7I	CTCGAG	2 cut(s)	933, 1265
PagI	TCATGA	1 cut(s)	796
PciI	ACATGT	1 cut(s)	1249
PciSI	GCTCTTC	1 cut(s)	1209
PcsI	WCGNNNNNNNCGW	3 cut(s)	38, 252, 302
PctI	GAATGC	1 cut(s)	990
PfeI	GAWTC	3 cut(s)	87, 505, 1087
PflFI	GACNNNGTC	1 cut(s)	352
PfoI	TCCNGGA	2 cut(s)	231, 1043
PkrI	GCNGC	5 cut(s)	19, 36, 193, 439, 1155
PscI	ACATGT	1 cut(s)	1249
Psp6I	CCWGG	2 cut(s)	231, 1303
PspGI	CCWGG	2 cut(s)	231, 1303
PspN4I	GGNNCC	4 cut(s)	65, 164, 381, 869
PspOMI	GGGCCC	1 cut(s)	162
PspPI	GGNCC	4 cut(s)	162, 163, 298, 380
PspXI	VCTCGAGB	2 cut(s)	933, 1265
PsyI	GACNNNGTC	1 cut(s)	352
RsaI	GTAC	3 cut(s)	11, 416, 677
RsaNI	GTAC	3 cut(s)	10, 415, 676
RseI	CAYNNNNRTG	3 cut(s)	714, 828, 983
Rsr2I	CGGWCCG	1 cut(s)	298
RsrII	CGGWCCG	1 cut(s)	298
SalI	GTCGAC	1 cut(s)	1068
SapI	GCTCTTC	1 cut(s)	1209
SaqAI	TTAA	3 cut(s)	576, 1103, 1182
SatI	GCNGC	5 cut(s)	18, 35, 192, 438, 1154
Sau3AI	GATC	7 cut(s)	114, 261, 697, 856, 1144, 1216, 1254
Sau96I	GGNCC	4 cut(s)	162, 163, 298, 380
ScrFI	CCNGG	7 cut(s)	160, 161, 233, 281, 302, 1045, 1305
SduI	GDGCHC	5 cut(s)	66, 166, 279, 911, 1192
SfaNI	GCATC	4 cut(s)	403, 651, 1213, 1303
SfiI	GGCCNNNNNGGCC	1 cut(s)	170
Sfr274I	CTCGAG	2 cut(s)	933, 1265
SinI	GGWCC	1 cut(s)	298
SlaI	CTCGAG	2 cut(s)	933, 1265
SmaI	CCCGGG	1 cut(s)	161
SmiMI	CAYNNNNRTG	3 cut(s)	714, 828, 983
SmlI	CTYRAG	2 cut(s)	933, 1265
SmoI	CTYRAG	2 cut(s)	933, 1265
Sse9I	AATT	3 cut(s)	572, 594, 852
SsiI	CCGC	8 cut(s)	17, 189, 192, 312, 339, 366, 437, 734
SspI	AATATT	1 cut(s)	760
SspMI	CTAG	2 cut(s)	840, 1091
StyD4I	CCNGG	7 cut(s)	158, 159, 231, 279, 300, 1043, 1303
StyI	CCWWGG	2 cut(s)	222, 1110
TaaI	ACNGT	3 cut(s)	500, 641, 920
TaiI	ACGT	3 cut(s)	258, 681, 1189
TaqI	TCGA	8 cut(s)	41, 258, 308, 826, 934, 1069, 1207, 1266
TasI	AATT	3 cut(s)	572, 594, 852
TauI	GCSGC	3 cut(s)	20, 194, 440
TfiI	GAWTC	3 cut(s)	87, 505, 1087
Tru1I	TTAA	3 cut(s)	576, 1103, 1182
Tru9I	TTAA	3 cut(s)	576, 1103, 1182
TscAI	CASTG	4 cut(s)	505, 517, 707, 869
TseFI	GTSAC	2 cut(s)	512, 925
TseI	GCWGC	2 cut(s)	34, 1153
Tsp45I	GTSAC	2 cut(s)	512, 925
TspDTI	ATGAA	5 cut(s)	698, 770, 785, 812, 1308
TspGWI	ACGGA	3 cut(s)	56, 740, 1277
TspMI	CCCGGG	1 cut(s)	159
TspRI	CASTG	4 cut(s)	505, 517, 707, 869
Tth111I	GACNNNGTC	1 cut(s)	352
VpaK11BI	GGWCC	1 cut(s)	298
XapI	RAATTY	1 cut(s)	572
XceI	RCATGY	2 cut(s)	1253, 1264
XhoI	CTCGAG	2 cut(s)	933, 1265
XmaI	CCCGGG	1 cut(s)	159
XmiI	GTMKAC	1 cut(s)	1069
XspI	CTAG	2 cut(s)	840, 1091

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.