Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0010174

Physical Location & Seq

Genomic Coordinates Forward (+)

32899466 .. 32913358

Gene Structure 13893 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1149500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1767 bp

ATGATGGTTACGGTGTCGTTGGATTTCCTAGTCCCGTCCTTGTGGGAGATCAAGGTCACTGTGGCTGCCTCCGTCTTCGTCATCTGCGCTTACTGGTTTTTCACATTCAGAGGCAGCGGAGGCGGCTCTGCTGATCGCCCACTTTCCGATAATTCCGGTGCGTTTGGGGATGCCATAGATGACAAAGACAAGATCCAGTTGAAAGGAGATCTTCAAAACAGTTCTGCATATCTAATCAAGCTGGAATTGTTGGCGGCTAAGAATCTCATTGGTGCAAACTTAAATGGGACATCGGATCCTTATGCTATGATCACTTGTGGAACAGAAAAGCGATTCAGTTCAATGGTCCCTGGTTCAAGAAATCCCATGTGGGGAGAGGAGTTTAATTTTTCTGTTGATGAGCTTCCGGTTCAGATTAATGTGACAATATATGACTGGGATATAATATGGAAAAGCACTGTTCTGGGTTCTGTGACTGTTCCAGTTGAAACCGAGGGTCAAACAGGTGCAGTATGGTATACATTGGATAGCCCATCAGGGCAGGTGTGTCTCCATATAAAAACAATAAAACTACCTGTTAACTCTCCAAGTGGCAGGGCAATGAATGGCTATGCTGGAGCGAATGCTCGAAGAAGGGTACCTTTAGATAGACAAGGGCCTACGGTGGTTCATCAAAAGCCAGGGCCTCTGCAAACTATATTTAATTTACTTCCAGATGAGGTTGTGGAGAATAGTTATTCATGTGCACTTGAGAGGTCATTCTTGTATCATGGCCGTATGTATGTCTCTACATGGCACATATGCTTCCACTCTAATGTGTTTTCTAAGCAAATGAAGGTCATCATACCGTTTGGTGATATTGATGAGATCCGGAGGAGTCAGCATGCATTCATTAATCCTGCTATAACAATTATACTGCGCATGGGTGCTGGTGGGCATGGTGTACCTCCTTTGGGGAGTCCCGATGGTAGAGTCAGATATAAATTTGCATCATTTTGGAACAGGAACCACGTGTTTAGAGGTCTATTGCGTGTAGAAAAGAATTACCATACAATGCTCGAAGCTGAGAAGAAGGAAAAGGCAGAGTCAGCACTGCGTGCGCATAGCAGTTCTGTAAAAGGAAGTCAGGCTAAGATTCCTGAAAATATCATCCCCAAAACTGGAAAGCTTCAAGCTTTTATCAAGGAGGAAGCTGTAACCAGCATATACACTGATGTTTTCCCGTGCACAGCTGAAAAGTTCTTCAATTTATTGTTGAGTGATGATTCAAATTATTTGCGTGAGTATCGTTCAGCACGAAAGGACACTAATCTTGTTATTGGACAGTGGCATCCTGCAGAGGAGTATGATGGTCAAGTCAGAGAAATAACATTTAGGTGCATTTGTAACAGTCCTATGTGCCCTCCAGATACAGCCATGACAGAATGGCAGCATGCTGTTCTATCACCGGACAAGATAACACTGGTGTTTGAGACGGTGCAACAGGCTCATGATGTTCCATTTGGGTCTTATTTTGAGGTCCATGTTAGGTGGAGCTTGGACACTATAGATGAGAACTCATGTAATATAGACATAAAAGTAGGTGTACATTTCAAGAAATGGTGTGTGATGCAATCCAAAATAAGGGCAGGGGCGATTAACGAGTACAAGAGAGAGATTGATCTGATGTTGGATATTGCCCGTTCATATATCATCAGGTCGAGTAGTTCAAATGGTGAGACTGACAAAGCCACGTCTTCACCCTCGGTAACTCGAGATAGTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0012505 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 DUF4782 GRAM

Protein Analysis

589

Amino Acids

65.8

Weight (kDa)

6.74

Isoelectric Point (pI)

42.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	80 - 175	7.7e-17	C2 domain
GRAM	PF02893	231 - 304	2.3e-16	GRAM domain
VASt	PF16016	402 - 547	4.2e-33	VAD1 Analog of StAR-related lipid transfer domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010174)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59660 AT3G59660
fragaria_vesca	FvH4_3g33330
malus_domestica	MD00G1149500.v1.1
prunus_persica	Prupe.1G207300_v2.0.a1 Prupe.1G207300_v2.0.a1 Prupe.1G207300_v2.0.a1 Prupe.1G207300_v2.0.a1 Prupe.1G207300_v2.0.a1
pyrus_communis	pycom13g26060
rosa_chinensis	RchiOBHm_Chr5g0060731
rosa_laevigata	RLG00000035392
rosa_roxburghii	Rroxscaffold_1G00019990 Rroxscaffold_3G00245380 Rroxscaffold_3G00245390
rosa_rugosa	Rorug05G0333300
rosa_samantha	Rh5AG396200 Rh5BG408300 Rh5CG433000 Rh5DG423400
rosa_wichuraiana	Rw5G037350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	532
Acc16I	TGCGCA	2 cut(s)	920, 1101
Acc36I	ACCTGC	1 cut(s)	532
Acc65I	GGTACC	1 cut(s)	637
AccB1I	GGYRCC	1 cut(s)	637
AccI	GTMKAC	1 cut(s)	518
AccIII	TCCGGA	1 cut(s)	870
AciI	CCGC	3 cut(s)	117, 123, 254
AclWI	GGATC	4 cut(s)	187, 290, 303, 862
AcoI	YGGCCR	1 cut(s)	772
AcsI	RAATTY	1 cut(s)	983
AcvI	CACGTG	1 cut(s)	1012
AdeI	CACNNNGTG	1 cut(s)	1097
AfaI	GTAC	4 cut(s)	639, 945, 1587, 1646
AfiI	CCNNNNNNNGG	4 cut(s)	371, 953, 1160, 1623
AflIII	ACRYGT	1 cut(s)	1011
AjiI	CACGTC	1 cut(s)	1734
AjnI	CCWGG	2 cut(s)	349, 679
AluBI	AGCT	8 cut(s)	241, 403, 1064, 1168, 1175, 1193, 1232, 1536
AluI	AGCT	8 cut(s)	241, 403, 1064, 1168, 1175, 1193, 1232, 1536
Alw21I	GWGCWC	2 cut(s)	748, 1229
Alw26I	GTCTC	4 cut(s)	554, 790, 1466, 1712
Alw44I	GTGCAC	2 cut(s)	744, 1225
AlwI	GGATC	4 cut(s)	187, 290, 303, 862
Ama87I	CYCGRG	1 cut(s)	1752
Aor13HI	TCCGGA	1 cut(s)	870
AoxI	GGCC	3 cut(s)	656, 683, 772
ApaLI	GTGCAC	2 cut(s)	744, 1225
ApeKI	GCWGC	3 cut(s)	65, 114, 1429
ApoI	RAATTY	1 cut(s)	983
AseI	ATTAAT	2 cut(s)	417, 894
Asp718I	GGTACC	1 cut(s)	637
AspLEI	GCGC	3 cut(s)	89, 921, 1102
AspS9I	GGNCC	4 cut(s)	346, 656, 683, 1519
AsuHPI	GGTGA	4 cut(s)	866, 1437, 1727, 1731
AvaI	CYCGRG	1 cut(s)	1752
AvaII	GGWCC	2 cut(s)	346, 1519
BaeGI	GKGCMC	3 cut(s)	748, 1229, 1403
BamHI	GGATCC	1 cut(s)	295
BanI	GGYRCC	1 cut(s)	637
BbrPI	CACGTG	1 cut(s)	1012
BbsI	GAAGAC	2 cut(s)	67, 1728
Bbv12I	GWGCWC	2 cut(s)	748, 1229
BbvI	GCAGC	3 cut(s)	52, 126, 1441
BccI	CCATC	3 cut(s)	541, 959, 1343
BceAI	ACGGC	1 cut(s)	759
BciT130I	CCWGG	2 cut(s)	351, 681
BclI	TGATCA	1 cut(s)	309
BcoDI	GTCTC	4 cut(s)	554, 790, 1466, 1712
BfaI	CTAG	1 cut(s)	29
BfmI	CTRYAG	2 cut(s)	1335, 1545
BfuAI	ACCTGC	1 cut(s)	532
BglII	AGATCT	1 cut(s)	208
BisI	GCNGC	5 cut(s)	66, 115, 124, 255, 1430
BlsI	GCNGC	5 cut(s)	67, 116, 125, 256, 1431
Bme1390I	CCNGG	2 cut(s)	351, 681
Bme18I	GGWCC	2 cut(s)	346, 1519
BmeT110I	CYCGRG	1 cut(s)	1752
BmgBI	CACGTC	1 cut(s)	1734
BmgT120I	GGNCC	4 cut(s)	346, 656, 683, 1519
BmiI	GGNNCC	4 cut(s)	297, 348, 639, 1007
BmrFI	CCNGG	2 cut(s)	351, 681
BmrI	ACTGGG	1 cut(s)	445
BmsI	GCATC	4 cut(s)	160, 998, 1339, 1599
BmuI	ACTGGG	1 cut(s)	445
BpiI	GAAGAC	2 cut(s)	67, 1728
BpmI	CTGGAG	2 cut(s)	636, 1389
BpuEI	CTTGAG	1 cut(s)	770
BsaAI	YACGTR	1 cut(s)	1012
BsaJI	CCNNGG	4 cut(s)	349, 492, 680, 1743
BsaWI	WCCGGW	4 cut(s)	155, 406, 870, 1447
Bsc4I	CCNNNNNNNGG	4 cut(s)	371, 953, 1160, 1623
Bse1I	ACTGG	6 cut(s)	98, 196, 440, 482, 1165, 1467
Bse3DI	GCAATG	1 cut(s)	606
BseAI	TCCGGA	1 cut(s)	870
BseBI	CCWGG	2 cut(s)	351, 681
BseDI	CCNNGG	4 cut(s)	349, 492, 680, 1743
BseGI	GGATG	3 cut(s)	175, 1149, 1330
BseLI	CCNNNNNNNGG	4 cut(s)	371, 953, 1160, 1623
BseMI	GCAATG	1 cut(s)	606
BseMII	CTCAG	1 cut(s)	1056
BseNI	ACTGG	6 cut(s)	98, 196, 440, 482, 1165, 1467
BseRI	GAGGAG	3 cut(s)	392, 889, 1355
BseSI	GKGCMC	3 cut(s)	748, 1229, 1403
BseXI	GCAGC	3 cut(s)	52, 126, 1441
BsgI	GTGCAG	1 cut(s)	528
BshFI	GGCC	3 cut(s)	658, 685, 774
BshNI	GGYRCC	1 cut(s)	637
BsiHKAI	GWGCWC	2 cut(s)	748, 1229
BsiHKCI	CYCGRG	1 cut(s)	1752
BsiSI	CCGG	4 cut(s)	156, 407, 871, 1448
BslFI	GGGAC	4 cut(s)	17, 301, 332, 945
BslI	CCNNNNNNNGG	4 cut(s)	371, 953, 1160, 1623
BsmAI	GTCTC	4 cut(s)	554, 790, 1466, 1712
BsmBI	CGTCTC	1 cut(s)	1466
BsmFI	GGGAC	4 cut(s)	17, 301, 332, 945
BsmI	GAATGC	2 cut(s)	628, 887
BsnI	GGCC	3 cut(s)	658, 685, 774
BsoBI	CYCGRG	1 cut(s)	1752
Bsp1286I	GDGCHC	3 cut(s)	748, 1229, 1403
Bsp13I	TCCGGA	1 cut(s)	870
Bsp1407I	TGTACA	1 cut(s)	1585
Bsp143I	GATC	8 cut(s)	48, 133, 192, 208, 295, 309, 867, 1660
BspACI	CCGC	3 cut(s)	117, 123, 254
BspANI	GGCC	3 cut(s)	658, 685, 774
BspCNI	CTCAG	1 cut(s)	1057
BspEI	TCCGGA	1 cut(s)	870
BspHI	TCATGA	1 cut(s)	1489
BspLI	GGNNCC	4 cut(s)	297, 348, 639, 1007
BspMAI	CTGCAG	1 cut(s)	1339
BspMI	ACCTGC	1 cut(s)	532
BspPI	GGATC	4 cut(s)	187, 290, 303, 862
BspT107I	GGYRCC	1 cut(s)	637
BsrDI	GCAATG	1 cut(s)	606
BsrGI	TGTACA	1 cut(s)	1585
BsrI	ACTGG	6 cut(s)	98, 196, 440, 482, 1165, 1467
BssECI	CCNNGG	4 cut(s)	349, 492, 680, 1743
BssMI	GATC	8 cut(s)	48, 133, 192, 208, 295, 309, 867, 1660
BssNAI	GTATAC	1 cut(s)	519
Bst1107I	GTATAC	1 cut(s)	519
Bst2UI	CCWGG	2 cut(s)	351, 681
BstAPI	GCANNNNNTGC	1 cut(s)	1097
BstAUI	TGTACA	1 cut(s)	1585
BstBAI	YACGTR	1 cut(s)	1012
BstC8I	GCNNGC	3 cut(s)	885, 1098, 1434
BstDEI	CTNAG	4 cut(s)	258, 825, 1065, 1131
BstF5I	GGATG	3 cut(s)	175, 1149, 1330
BstHHI	GCGC	3 cut(s)	89, 921, 1102
BstKTI	GATC	8 cut(s)	51, 136, 195, 211, 298, 312, 870, 1663
BstMAI	GTCTC	4 cut(s)	554, 790, 1466, 1712
BstMBI	GATC	8 cut(s)	48, 133, 192, 208, 295, 309, 867, 1660
BstMWI	GCNNNNNNNGC	4 cut(s)	120, 123, 1088, 1097
BstNI	CCWGG	2 cut(s)	351, 681
BstNSI	RCATGY	2 cut(s)	887, 1436
BstSCI	CCNGG	2 cut(s)	349, 679
BstSFI	CTRYAG	2 cut(s)	1335, 1545
BstSLI	GKGCMC	3 cut(s)	748, 1229, 1403
BstV1I	GCAGC	3 cut(s)	52, 126, 1441
BstV2I	GAAGAC	2 cut(s)	67, 1728
BstX2I	RGATCY	4 cut(s)	192, 208, 295, 867
BstYI	RGATCY	4 cut(s)	192, 208, 295, 867
BstZ17I	GTATAC	1 cut(s)	519
BsuRI	GGCC	3 cut(s)	658, 685, 774
BtrI	CACGTC	1 cut(s)	1734
BtsCI	GGATG	3 cut(s)	175, 1149, 1330
BtsI	GCAGTG	1 cut(s)	1091
BtsIMutI	CAGTG	6 cut(s)	57, 456, 1091, 1209, 1331, 1460
BveI	ACCTGC	1 cut(s)	532
Cac8I	GCNNGC	3 cut(s)	885, 1098, 1434
CciI	TCATGA	1 cut(s)	1489
CfoI	GCGC	3 cut(s)	89, 921, 1102
Cfr13I	GGNCC	4 cut(s)	346, 656, 683, 1519
Csp6I	GTAC	4 cut(s)	638, 944, 1586, 1645
CviQI	GTAC	4 cut(s)	638, 944, 1586, 1645
DdeI	CTNAG	4 cut(s)	258, 825, 1065, 1131
DpnI	GATC	8 cut(s)	50, 135, 194, 210, 297, 311, 869, 1662
DpnII	GATC	8 cut(s)	48, 133, 192, 208, 295, 309, 867, 1660
DraIII	CACNNNGTG	1 cut(s)	1097
EaeI	YGGCCR	1 cut(s)	772
Eco47I	GGWCC	2 cut(s)	346, 1519
Eco72I	CACGTG	1 cut(s)	1012
Eco88I	CYCGRG	1 cut(s)	1752
EcoO109I	RGGNCCY	2 cut(s)	656, 683
EcoRII	CCWGG	2 cut(s)	349, 679
EcoT22I	ATGCAT	1 cut(s)	889
Esp3I	CGTCTC	1 cut(s)	1466
FalI	AAGNNNNNCTT	4 cut(s)	195, 227, 625, 657
FaqI	GGGAC	4 cut(s)	17, 301, 332, 945
FauNDI	CATATG	1 cut(s)	800
FbaI	TGATCA	1 cut(s)	309
FblI	GTMKAC	1 cut(s)	518
Fnu4HI	GCNGC	5 cut(s)	66, 115, 124, 255, 1430
FokI	GGATG	3 cut(s)	182, 1136, 1317
Fsp4HI	GCNGC	5 cut(s)	66, 115, 124, 255, 1430
FspAI	RTGCGCAY	1 cut(s)	1101
FspBI	CTAG	1 cut(s)	29
FspI	TGCGCA	2 cut(s)	920, 1101
GlaI	GCGC	3 cut(s)	88, 920, 1101
GluI	GCNGC	5 cut(s)	66, 115, 124, 255, 1430
GsuI	CTGGAG	2 cut(s)	636, 1389
HaeIII	GGCC	3 cut(s)	658, 685, 774
HapII	CCGG	4 cut(s)	156, 407, 871, 1448
HhaI	GCGC	3 cut(s)	89, 921, 1102
Hin6I	GCGC	3 cut(s)	87, 919, 1100
HinP1I	GCGC	3 cut(s)	87, 919, 1100
HincII	GTYRAC	1 cut(s)	580
HindII	GTYRAC	1 cut(s)	580
HindIII	AAGCTT	2 cut(s)	1166, 1173
HinfI	GANTC	8 cut(s)	262, 333, 877, 958, 972, 1085, 1135, 1265
HpaI	GTTAAC	1 cut(s)	580
HpaII	CCGG	4 cut(s)	156, 407, 871, 1448
HphI	GGTGA	4 cut(s)	866, 1437, 1727, 1731
Hpy166II	GTNNAC	6 cut(s)	519, 580, 746, 944, 1227, 1586
Hpy188I	TCNGA	7 cut(s)	110, 148, 295, 414, 977, 1361, 1665
Hpy188III	TCNNGA	9 cut(s)	357, 713, 871, 962, 1139, 1406, 1490, 1594, 1754
Hpy8I	GTNNAC	6 cut(s)	519, 580, 746, 944, 1227, 1586
HpyAV	CCTTC	3 cut(s)	627, 829, 1066
HpyCH4IV	ACGT	2 cut(s)	1011, 1733
HpyF10VI	GCNNNNNNNGC	4 cut(s)	120, 123, 1088, 1097
HpyF3I	CTNAG	4 cut(s)	258, 825, 1065, 1131
HpySE526I	ACGT	2 cut(s)	1011, 1733
HspAI	GCGC	3 cut(s)	87, 919, 1100
Kpn2I	TCCGGA	1 cut(s)	870
KpnI	GGTACC	1 cut(s)	641
Ksp22I	TGATCA	1 cut(s)	309
KspAI	GTTAAC	1 cut(s)	580
Kzo9I	GATC	8 cut(s)	48, 133, 192, 208, 295, 309, 867, 1660
LmnI	GCTCC	2 cut(s)	617, 1533
Lsp1109I	GCAGC	3 cut(s)	52, 126, 1441
LweI	GCATC	4 cut(s)	160, 998, 1339, 1599
MaeI	CTAG	1 cut(s)	29
MaeII	ACGT	2 cut(s)	1011, 1733
MaeIII	GTNAC	7 cut(s)	7, 55, 421, 472, 1195, 1385, 1747
MalI	GATC	8 cut(s)	50, 135, 194, 210, 297, 311, 869, 1662
MboI	GATC	8 cut(s)	48, 133, 192, 208, 295, 309, 867, 1660
MboII	GAAGA	6 cut(s)	67, 203, 642, 1081, 1234, 1728
MflI	RGATCY	4 cut(s)	192, 208, 295, 867
MhlI	GDGCHC	3 cut(s)	748, 1229, 1403
MluCI	AATT	9 cut(s)	151, 245, 385, 703, 909, 983, 1042, 1246, 1270
MlyI	GAGTC	4 cut(s)	886, 967, 981, 1094
MmeI	TCCRAC	1 cut(s)	1650
Mph1103I	ATGCAT	1 cut(s)	889
MroI	TCCGGA	1 cut(s)	870
MseI	TTAA	8 cut(s)	281, 384, 417, 579, 702, 894, 1638, 1765
MslI	CAYNNNNRTG	2 cut(s)	813, 1375
MspA1I	CMGCKG	2 cut(s)	117, 1232
MspI	CCGG	4 cut(s)	156, 407, 871, 1448
MspR9I	CCNGG	2 cut(s)	351, 681
Mva1269I	GAATGC	2 cut(s)	628, 887
MvaI	CCWGG	2 cut(s)	351, 681
MwoI	GCNNNNNNNGC	4 cut(s)	120, 123, 1088, 1097
NdeI	CATATG	1 cut(s)	800
NdeII	GATC	8 cut(s)	48, 133, 192, 208, 295, 309, 867, 1660
NlaIV	GGNNCC	4 cut(s)	297, 348, 639, 1007
NmuCI	GTSAC	3 cut(s)	55, 421, 472
NsbI	TGCGCA	2 cut(s)	920, 1101
NsiI	ATGCAT	1 cut(s)	889
NspI	RCATGY	2 cut(s)	887, 1436
PaeI	GCATGC	2 cut(s)	887, 1436
PaeR7I	CTCGAG	1 cut(s)	1752
PagI	TCATGA	1 cut(s)	1489
PaqCI	CACCTGC	1 cut(s)	532
PcsI	WCGNNNNNNNCGW	1 cut(s)	1294
PctI	GAATGC	2 cut(s)	628, 887
PfeI	GAWTC	4 cut(s)	262, 333, 1135, 1265
PkrI	GCNGC	5 cut(s)	67, 116, 125, 256, 1431
PleI	GAGTC	4 cut(s)	885, 966, 980, 1093
PmaCI	CACGTG	1 cut(s)	1012
PmlI	CACGTG	1 cut(s)	1012
PpsI	GAGTC	4 cut(s)	885, 966, 980, 1093
Ppu21I	YACGTR	1 cut(s)	1012
PshBI	ATTAAT	2 cut(s)	417, 894
Psp6I	CCWGG	2 cut(s)	349, 679
PspCI	CACGTG	1 cut(s)	1012
PspGI	CCWGG	2 cut(s)	349, 679
PspN4I	GGNNCC	4 cut(s)	297, 348, 639, 1007
PspPI	GGNCC	4 cut(s)	346, 656, 683, 1519
PstI	CTGCAG	1 cut(s)	1339
PsuI	RGATCY	4 cut(s)	192, 208, 295, 867
PvuII	CAGCTG	1 cut(s)	1232
RsaI	GTAC	4 cut(s)	639, 945, 1587, 1646
RsaNI	GTAC	4 cut(s)	638, 944, 1586, 1645
RseI	CAYNNNNRTG	2 cut(s)	813, 1375
SaqAI	TTAA	8 cut(s)	281, 384, 417, 579, 702, 894, 1638, 1765
SatI	GCNGC	5 cut(s)	66, 115, 124, 255, 1430
Sau3AI	GATC	8 cut(s)	48, 133, 192, 208, 295, 309, 867, 1660
Sau96I	GGNCC	4 cut(s)	346, 656, 683, 1519
SchI	GAGTC	4 cut(s)	886, 967, 981, 1094
ScrFI	CCNGG	2 cut(s)	351, 681
SduI	GDGCHC	3 cut(s)	748, 1229, 1403
SfaNI	GCATC	4 cut(s)	160, 998, 1339, 1599
SfcI	CTRYAG	2 cut(s)	1335, 1545
Sfr274I	CTCGAG	1 cut(s)	1752
SinI	GGWCC	2 cut(s)	346, 1519
SlaI	CTCGAG	1 cut(s)	1752
SmiMI	CAYNNNNRTG	2 cut(s)	813, 1375
SmlI	CTYRAG	2 cut(s)	749, 1752
SmoI	CTYRAG	2 cut(s)	749, 1752
SphI	GCATGC	2 cut(s)	887, 1436
Sse9I	AATT	9 cut(s)	151, 245, 385, 703, 909, 983, 1042, 1246, 1270
SsiI	CCGC	3 cut(s)	117, 123, 254
SspMI	CTAG	1 cut(s)	29
StyD4I	CCNGG	2 cut(s)	349, 679
TaiI	ACGT	2 cut(s)	1014, 1736
TaqI	TCGA	4 cut(s)	628, 1059, 1700, 1753
TasI	AATT	9 cut(s)	151, 245, 385, 703, 909, 983, 1042, 1246, 1270
TatI	WGTACW	2 cut(s)	1585, 1644
TauI	GCSGC	2 cut(s)	126, 257
TfiI	GAWTC	4 cut(s)	262, 333, 1135, 1265
Tru1I	TTAA	8 cut(s)	281, 384, 417, 579, 702, 894, 1638, 1765
Tru9I	TTAA	8 cut(s)	281, 384, 417, 579, 702, 894, 1638, 1765
TscAI	CASTG	6 cut(s)	64, 463, 1098, 1216, 1331, 1467
TseFI	GTSAC	3 cut(s)	55, 421, 472
TseI	GCWGC	3 cut(s)	65, 114, 1429
Tsp45I	GTSAC	3 cut(s)	55, 421, 472
TspDTI	ATGAA	6 cut(s)	617, 659, 729, 848, 880, 1674
TspGWI	ACGGA	1 cut(s)	61
TspRI	CASTG	6 cut(s)	64, 463, 1098, 1216, 1331, 1467
VneI	GTGCAC	2 cut(s)	744, 1225
VpaK11BI	GGWCC	2 cut(s)	346, 1519
VspI	ATTAAT	2 cut(s)	417, 894
XapI	RAATTY	1 cut(s)	983
XceI	RCATGY	2 cut(s)	887, 1436
XhoI	CTCGAG	1 cut(s)	1752
XmiI	GTMKAC	1 cut(s)	518
XspI	CTAG	1 cut(s)	29
Zsp2I	ATGCAT	1 cut(s)	889

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.