Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0010174

Physical Location & Seq

Genomic Coordinates Reverse (-)

66248760 .. 66259733

Gene Structure 10974 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG408300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGAACATCTTTTATATAGTTCATGAGTTTAACAATCAGAATTTGAAAGCCATCATGGAAACTATGTCGTTGGATTTCCTTGTGCCGTCCTTGTGGGAGATCAAGGTTACAGTGGCTGCCTCCATCTATGTGATCTTCGCTTACTGGTTCTTCACGTACACAGGCGGCGGAGATGGCTACTGTGATCGCTCACTTGCTGACAATTCGGGTGAATCTGGGGATGCCATAGAGGAGAAGGATGGCACGTTGAAAGGGGATGCACACAACAATTCTCCATATTTAATCAAGGTTGAGCTGTTAGCTGCTAAGAATCTTATTGGTGCAAACTTAAATGGCACGTCGGATCCATATGCTATCATCACATGTGGAACAGAAAAGCGATTTAGTTCAATGGTCCCTGGTTCAAGAAATCCCATGTGGGGAGAGGAGTTTAATTTTTCTGTTGATGAGCTTCCTGTCCAGATTAATGTGACAATATATGACTGGGATATTATATGGAAAAGCACAGTTCTTGGTTCAGTGACTGTTCCAGTTGAAAGTGAAGGTCAAACAGGTGCTTTGTGGTATACATTAGATAGCCCATCGGGTCAGGTTTGTCTCCATATAAAATCAATAAAACTTCCCGTAACTTCTCCAAGTGGTAGGGTTGTGAATGGTTATGGTGGTGGCCGAAGGACATCTTTAGATAAACAAGAACCCACAGTTGTTCATCAAAAGCCAGGACCCCTTCAAACCATATTTGATCTACTTCCAGATGAGATTGTGGAACATAGTTATTCATGTGCACTTGAGAGGTCGTTCTTATATCATGGCCGTATGTATGTCTCTGCATGGCACATTTGCTTCCACTCTAATGTGTTTTCTAAGCAAATGAAGGTGCTCATACCAGTTGGTGATATTGATGAGATTCGGAGGACTCAACATGCATTCATTAATCCTGCTGTAACAATTATACTCCGCATGGGTGCCGGTGGGCATGGTGTACCTCCTTTGGGGAGTCCTGATGGTAGAGTCAGGTATAAATTTGCATCATTTTGGAACAGGAATCATGCATTCAGAGCTCTTCAGCGTGCAAGAAAGAATTACCATGGAATGCTAGAAGCTGAAAAGAAGGAGAAGGCAGAGTCAGCGTTGCGTGCACATAGCAGTTCTATAAGAGGAAGTAAAAGTCAGGCTATGATTCCTGAAGATATCATCCCCAATACTGGGAAGCGCCAAGCTTTTATCAAGGATGAAGCTTTGAACAGTATATTTAATGATACTTTTCCCTGCACAGCTGAGAATTACTTCAATTTATTGTTGAGTGATAATTCAAATTTTACAAACGAGTACCGTTCAGCACGAAAGGATACTAATCTTGTAATTGGACATTGGCATCCAGCAGATGAATATGATGGTCAAGTAAGAGAAATAACATTCAGGGGTCTTTGTAACAGTCCCATGTGTCCTCCGGACACTGCCATGACAGAGTGGCAGCATGCTGTTTTGTCACCTGACAAGGAAACACTGGTGTTTGAGACAGTACAACAGGCTCATGATGTTCCATTTGGTTCTTACTTTGAGATCCATTGTAGGTGGAAGTTGGAGACTAACGCTGAAAACTCATGTATCATAGATATAAAAGTGGGTGCACATTTTAAGAAATGGTGTCCGATGCAGTCTAAAATAAAGTCGAGCGCTATTAATGAGTACAAGAAAGAGGTTGAGGTGATGCTGGAAGTTGCCCGCTCCTACATTAAGTCAAGTACTTCGGCCGGTGAGACTGACAAAGTCACTTCCTCACCCTCTCAAGAGAGCAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0012505 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 DUF4782 GRAM

Protein Analysis

600

Amino Acids

67.24

Weight (kDa)

6.04

Isoelectric Point (pI)

41.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	95 - 191	3.2e-17	C2 domain
GRAM	PF02893	244 - 310	1.4e-14	GRAM domain
VASt	PF16016	417 - 562	1.7e-30	VAD1 Analog of StAR-related lipid transfer domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010174)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59660 AT3G59660
fragaria_vesca	FvH4_3g33330
malus_domestica	MD00G1149500.v1.1
prunus_persica	Prupe.1G207300_v2.0.a1 Prupe.1G207300_v2.0.a1 Prupe.1G207300_v2.0.a1 Prupe.1G207300_v2.0.a1 Prupe.1G207300_v2.0.a1
pyrus_communis	pycom13g26060
rosa_chinensis	RchiOBHm_Chr5g0060731
rosa_laevigata	RLG00000035392
rosa_roxburghii	Rroxscaffold_1G00019990 Rroxscaffold_3G00245380 Rroxscaffold_3G00245390
rosa_rugosa	Rorug05G0333300
rosa_samantha	Rh5AG396200 Rh5BG408300 Rh5CG433000 Rh5DG423400
rosa_wichuraiana	Rw5G037350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	965
AccBSI	CCGCTC	1 cut(s)	1728
AccI	GTMKAC	1 cut(s)	566
AccIII	TCCGGA	1 cut(s)	1450
AciI	CCGC	4 cut(s)	165, 168, 958, 1726
AclWI	GGATC	3 cut(s)	338, 351, 1558
AcoI	YGGCCR	3 cut(s)	667, 811, 1752
AcsI	RAATTY	3 cut(s)	40, 1022, 1315
AcuI	CTGAAG	2 cut(s)	1049, 1206
AfaI	GTAC	6 cut(s)	158, 984, 1331, 1524, 1691, 1747
AfeI	AGCGCT	1 cut(s)	1678
AfiI	CCNNNNNNNGG	4 cut(s)	419, 992, 1205, 1206
AflIII	ACRYGT	1 cut(s)	362
AgsI	TTSAA	9 cut(s)	46, 250, 390, 405, 536, 731, 1243, 1291, 1314
AjiI	CACGTC	1 cut(s)	339
AjnI	CCWGG	2 cut(s)	397, 718
AluBI	AGCT	9 cut(s)	295, 302, 451, 1061, 1103, 1220, 1238, 1277, 1800
AluI	AGCT	9 cut(s)	295, 302, 451, 1061, 1103, 1220, 1238, 1277, 1800
Alw21I	GWGCWC	5 cut(s)	787, 882, 1063, 1141, 1633
Alw26I	GTCTC	5 cut(s)	602, 829, 1511, 1580, 1754
Alw44I	GTGCAC	3 cut(s)	783, 1137, 1629
AlwI	GGATC	3 cut(s)	338, 351, 1558
AlwNI	CAGNNNCTG	2 cut(s)	116, 524
Aor13HI	TCCGGA	1 cut(s)	1450
Aor51HI	AGCGCT	1 cut(s)	1678
AoxI	GGCC	3 cut(s)	667, 811, 1752
ApaLI	GTGCAC	3 cut(s)	783, 1137, 1629
ApeKI	GCWGC	4 cut(s)	116, 302, 1474, 1797
ApoI	RAATTY	3 cut(s)	40, 1022, 1315
AseI	ATTAAT	3 cut(s)	465, 933, 1683
Asp700I	GAANNNNTTC	1 cut(s)	1286
AspLEI	GCGC	2 cut(s)	1215, 1679
AspS9I	GGNCC	2 cut(s)	394, 722
AsuHPI	GGTGA	6 cut(s)	221, 905, 1482, 1720, 1769, 1773
AvaII	GGWCC	2 cut(s)	394, 722
BaeGI	GKGCMC	3 cut(s)	787, 1141, 1633
BaeI	ACNNNNGTAYC	2 cut(s)	1591, 1624
BamHI	GGATCC	1 cut(s)	343
BanI	GGYRCC	1 cut(s)	965
BanII	GRGCYC	1 cut(s)	1063
Bbv12I	GWGCWC	5 cut(s)	787, 882, 1063, 1141, 1633
BbvI	GCAGC	3 cut(s)	103, 289, 1486
BccI	CCATC	7 cut(s)	59, 131, 167, 233, 589, 998, 1388
BceAI	ACGGC	2 cut(s)	70, 798
BciT130I	CCWGG	2 cut(s)	399, 720
BciVI	GTATCC	1 cut(s)	1342
BcoDI	GTCTC	5 cut(s)	602, 829, 1511, 1580, 1754
BfaI	CTAG	1 cut(s)	1097
BfoI	RGCGCY	2 cut(s)	1216, 1680
BfuI	GTATCC	1 cut(s)	1342
BisI	GCNGC	5 cut(s)	117, 166, 303, 1475, 1798
BlsI	GCNGC	5 cut(s)	118, 167, 304, 1476, 1799
BmcAI	AGTACT	1 cut(s)	1747
Bme1390I	CCNGG	2 cut(s)	399, 720
Bme18I	GGWCC	2 cut(s)	394, 722
BmgBI	CACGTC	1 cut(s)	339
BmgT120I	GGNCC	2 cut(s)	394, 722
BmiI	GGNNCC	4 cut(s)	345, 396, 724, 967
BmrFI	CCNGG	2 cut(s)	399, 720
BmrI	ACTGGG	2 cut(s)	493, 1215
BmsI	GCATC	6 cut(s)	211, 247, 1037, 1384, 1644, 1701
BmuI	ACTGGG	2 cut(s)	493, 1215
BpuEI	CTTGAG	2 cut(s)	809, 1773
BsaAI	YACGTR	1 cut(s)	156
BsaBI	GATNNNNATC	1 cut(s)	1353
BsaJI	CCNNGG	2 cut(s)	397, 1087
BsaWI	WCCGGW	1 cut(s)	1450
Bsc4I	CCNNNNNNNGG	4 cut(s)	419, 992, 1205, 1206
Bse118I	RCCGGY	2 cut(s)	968, 1754
Bse1I	ACTGG	6 cut(s)	149, 488, 530, 887, 1210, 1512
Bse8I	GATNNNNATC	1 cut(s)	1353
BseAI	TCCGGA	1 cut(s)	1450
BseBI	CCWGG	2 cut(s)	399, 720
BseDI	CCNNGG	2 cut(s)	397, 1087
BseGI	GGATG	6 cut(s)	226, 244, 262, 1194, 1237, 1375
BseJI	GATNNNNATC	1 cut(s)	1353
BseLI	CCNNNNNNNGG	4 cut(s)	419, 992, 1205, 1206
BseMII	CTCAG	1 cut(s)	1269
BseNI	ACTGG	6 cut(s)	149, 488, 530, 887, 1210, 1512
BseRI	GAGGAG	2 cut(s)	245, 440
BseSI	GKGCMC	3 cut(s)	787, 1141, 1633
BseX3I	CGGCCG	1 cut(s)	1752
BseXI	GCAGC	3 cut(s)	103, 289, 1486
BsgI	GTGCAG	1 cut(s)	1255
Bsh1285I	CGRYCG	1 cut(s)	1755
BshFI	GGCC	3 cut(s)	669, 813, 1754
BshNI	GGYRCC	1 cut(s)	965
BsiEI	CGRYCG	1 cut(s)	1755
BsiHKAI	GWGCWC	5 cut(s)	787, 882, 1063, 1141, 1633
BsiSI	CCGG	3 cut(s)	969, 1451, 1755
BslFI	GGGAC	2 cut(s)	380, 1422
BslI	CCNNNNNNNGG	4 cut(s)	419, 992, 1205, 1206
BsmAI	GTCTC	5 cut(s)	602, 829, 1511, 1580, 1754
BsmFI	GGGAC	2 cut(s)	380, 1422
BsmI	GAATGC	3 cut(s)	926, 1052, 1098
BsnI	GGCC	3 cut(s)	669, 813, 1754
Bsp1286I	GDGCHC	5 cut(s)	787, 882, 1063, 1141, 1633
Bsp13I	TCCGGA	1 cut(s)	1450
Bsp143I	GATC	6 cut(s)	99, 132, 184, 343, 742, 1563
Bsp19I	CCATGG	1 cut(s)	1087
BspACI	CCGC	4 cut(s)	165, 168, 958, 1726
BspANI	GGCC	3 cut(s)	669, 813, 1754
BspCNI	CTCAG	1 cut(s)	1270
BspEI	TCCGGA	1 cut(s)	1450
BspHI	TCATGA	2 cut(s)	22, 1534
BspLI	GGNNCC	4 cut(s)	345, 396, 724, 967
BspPI	GGATC	3 cut(s)	338, 351, 1558
BspQI	GCTCTTC	1 cut(s)	1068
BspT107I	GGYRCC	1 cut(s)	965
BsrBI	CCGCTC	1 cut(s)	1728
BsrFI	RCCGGY	2 cut(s)	968, 1754
BsrI	ACTGG	6 cut(s)	149, 488, 530, 887, 1210, 1512
BssAI	RCCGGY	2 cut(s)	968, 1754
BssECI	CCNNGG	2 cut(s)	397, 1087
BssMI	GATC	6 cut(s)	99, 132, 184, 343, 742, 1563
BssNAI	GTATAC	1 cut(s)	567
BssT1I	CCWWGG	1 cut(s)	1087
Bst1107I	GTATAC	1 cut(s)	567
Bst2UI	CCWGG	2 cut(s)	399, 720
Bst4CI	ACNGT	9 cut(s)	112, 182, 508, 526, 703, 1247, 1334, 1436, 1522
Bst6I	CTCTTC	1 cut(s)	1068
BstBAI	YACGTR	1 cut(s)	156
BstC8I	GCNNGC	4 cut(s)	1071, 1137, 1479, 1726
BstDEI	CTNAG	3 cut(s)	306, 864, 1278
BstDSI	CCRYGG	1 cut(s)	1087
BstF5I	GGATG	6 cut(s)	226, 244, 262, 1194, 1237, 1375
BstH2I	RGCGCY	2 cut(s)	1216, 1680
BstHHI	GCGC	2 cut(s)	1215, 1679
BstKTI	GATC	6 cut(s)	102, 135, 187, 346, 745, 1566
BstMAI	GTCTC	5 cut(s)	602, 829, 1511, 1580, 1754
BstMBI	GATC	6 cut(s)	99, 132, 184, 343, 742, 1563
BstMCI	CGRYCG	1 cut(s)	1755
BstMWI	GCNNNNNNNGC	4 cut(s)	174, 1058, 1127, 1136
BstNI	CCWGG	2 cut(s)	399, 720
BstNSI	RCATGY	3 cut(s)	366, 926, 1481
BstSCI	CCNGG	2 cut(s)	397, 718
BstSLI	GKGCMC	3 cut(s)	787, 1141, 1633
BstV1I	GCAGC	3 cut(s)	103, 289, 1486
BstX2I	RGATCY	2 cut(s)	343, 1563
BstYI	RGATCY	2 cut(s)	343, 1563
BstZ17I	GTATAC	1 cut(s)	567
BstZI	CGGCCG	1 cut(s)	1752
BsuI	GTATCC	1 cut(s)	1342
BsuRI	GGCC	3 cut(s)	669, 813, 1754
BtgI	CCRYGG	1 cut(s)	1087
BtrI	CACGTC	1 cut(s)	339
BtsCI	GGATG	6 cut(s)	226, 244, 262, 1194, 1237, 1375
BtsI	GCAGTG	1 cut(s)	1455
BtsIMutI	CAGTG	4 cut(s)	117, 525, 1455, 1505
Cac8I	GCNNGC	4 cut(s)	1071, 1137, 1479, 1726
CaiI	CAGNNNCTG	2 cut(s)	116, 524
CciI	TCATGA	2 cut(s)	22, 1534
CfoI	GCGC	2 cut(s)	1215, 1679
Cfr10I	RCCGGY	2 cut(s)	968, 1754
Cfr13I	GGNCC	2 cut(s)	394, 722
Csp6I	GTAC	6 cut(s)	157, 983, 1330, 1523, 1690, 1746
CviQI	GTAC	6 cut(s)	157, 983, 1330, 1523, 1690, 1746
DdeI	CTNAG	3 cut(s)	306, 864, 1278
DpnI	GATC	6 cut(s)	101, 134, 186, 345, 744, 1565
DpnII	GATC	6 cut(s)	99, 132, 184, 343, 742, 1563
EaeI	YGGCCR	3 cut(s)	667, 811, 1752
EagI	CGGCCG	1 cut(s)	1752
Eam1104I	CTCTTC	1 cut(s)	1068
EarI	CTCTTC	1 cut(s)	1068
EciI	GGCGGA	1 cut(s)	183
Ecl136II	GAGCTC	1 cut(s)	1061
EclXI	CGGCCG	1 cut(s)	1752
Eco130I	CCWWGG	1 cut(s)	1087
Eco24I	GRGCYC	1 cut(s)	1063
Eco32I	GATATC	1 cut(s)	1192
Eco47I	GGWCC	2 cut(s)	394, 722
Eco47III	AGCGCT	1 cut(s)	1678
Eco52I	CGGCCG	1 cut(s)	1752
Eco53kI	GAGCTC	1 cut(s)	1061
Eco57I	CTGAAG	2 cut(s)	1049, 1206
EcoICRI	GAGCTC	1 cut(s)	1061
EcoO109I	RGGNCCY	1 cut(s)	722
EcoRII	CCWGG	2 cut(s)	397, 718
EcoRV	GATATC	1 cut(s)	1192
EcoT14I	CCWWGG	1 cut(s)	1087
EcoT22I	ATGCAT	2 cut(s)	928, 1054
EcoT38I	GRGCYC	1 cut(s)	1063
ErhI	CCWWGG	1 cut(s)	1087
FalI	AAGNNNNNCTT	2 cut(s)	664, 696
FaqI	GGGAC	2 cut(s)	380, 1422
FauI	CCCGC	1 cut(s)	1733
FauNDI	CATATG	1 cut(s)	349
FblI	GTMKAC	1 cut(s)	566
Fnu4HI	GCNGC	5 cut(s)	117, 166, 303, 1475, 1798
FokI	GGATG	6 cut(s)	233, 251, 269, 1181, 1244, 1362
FriOI	GRGCYC	1 cut(s)	1063
Fsp4HI	GCNGC	5 cut(s)	117, 166, 303, 1475, 1798
FspBI	CTAG	1 cut(s)	1097
GlaI	GCGC	2 cut(s)	1214, 1678
GluI	GCNGC	5 cut(s)	117, 166, 303, 1475, 1798
HaeII	RGCGCY	2 cut(s)	1216, 1680
HaeIII	GGCC	3 cut(s)	669, 813, 1754
HapII	CCGG	3 cut(s)	969, 1451, 1755
HhaI	GCGC	2 cut(s)	1215, 1679
Hin6I	GCGC	2 cut(s)	1213, 1677
HinP1I	GCGC	2 cut(s)	1213, 1677
HindIII	AAGCTT	2 cut(s)	1218, 1236
HinfI	GANTC	9 cut(s)	212, 310, 907, 916, 997, 1011, 1045, 1124, 1180
HpaII	CCGG	3 cut(s)	969, 1451, 1755
HphI	GGTGA	6 cut(s)	221, 905, 1482, 1720, 1769, 1773
Hpy166II	GTNNAC	6 cut(s)	159, 567, 785, 983, 1139, 1631
Hpy188I	TCNGA	5 cut(s)	39, 343, 912, 1058, 1653
Hpy188III	TCNNGA	9 cut(s)	23, 405, 460, 752, 1001, 1184, 1451, 1535, 1790
Hpy8I	GTNNAC	6 cut(s)	159, 567, 785, 983, 1139, 1631
Hpy99I	CGWCG	1 cut(s)	343
HpyAV	CCTTC	7 cut(s)	229, 536, 666, 737, 868, 1105, 1111
HpyCH4III	ACNGT	9 cut(s)	112, 182, 508, 526, 703, 1247, 1334, 1436, 1522
HpyCH4IV	ACGT	3 cut(s)	155, 245, 338
HpyF10VI	GCNNNNNNNGC	4 cut(s)	174, 1058, 1127, 1136
HpyF3I	CTNAG	3 cut(s)	306, 864, 1278
HpySE526I	ACGT	3 cut(s)	155, 245, 338
HspAI	GCGC	2 cut(s)	1213, 1677
Kpn2I	TCCGGA	1 cut(s)	1450
Kzo9I	GATC	6 cut(s)	99, 132, 184, 343, 742, 1563
LguI	GCTCTTC	1 cut(s)	1068
LmnI	GCTCC	1 cut(s)	1733
Lsp1109I	GCAGC	3 cut(s)	103, 289, 1486
LweI	GCATC	6 cut(s)	211, 247, 1037, 1384, 1644, 1701
MaeI	CTAG	1 cut(s)	1097
MaeII	ACGT	3 cut(s)	155, 245, 338
MaeIII	GTNAC	8 cut(s)	106, 469, 520, 625, 943, 1430, 1488, 1771
MalI	GATC	6 cut(s)	101, 134, 186, 345, 744, 1565
MbiI	CCGCTC	1 cut(s)	1728
MboI	GATC	6 cut(s)	99, 132, 184, 343, 742, 1563
MboII	GAAGA	4 cut(s)	127, 142, 1055, 1199
MflI	RGATCY	2 cut(s)	343, 1563
MhlI	GDGCHC	5 cut(s)	787, 882, 1063, 1141, 1633
MlyI	GAGTC	4 cut(s)	910, 1006, 1020, 1133
MmeI	TCCRAC	3 cut(s)	51, 321, 1563
Mph1103I	ATGCAT	2 cut(s)	928, 1054
MroI	TCCGGA	1 cut(s)	1450
MroXI	GAANNNNTTC	1 cut(s)	1286
MslI	CAYNNNNRTG	3 cut(s)	128, 852, 1460
MspA1I	CMGCKG	1 cut(s)	1277
MspI	CCGG	3 cut(s)	969, 1451, 1755
MspR9I	CCNGG	2 cut(s)	399, 720
Mva1269I	GAATGC	3 cut(s)	926, 1052, 1098
MvaI	CCWGG	2 cut(s)	399, 720
MwoI	GCNNNNNNNGC	4 cut(s)	174, 1058, 1127, 1136
NcoI	CCATGG	1 cut(s)	1087
NdeI	CATATG	1 cut(s)	349
NdeII	GATC	6 cut(s)	99, 132, 184, 343, 742, 1563
NlaIV	GGNNCC	4 cut(s)	345, 396, 724, 967
NmuCI	GTSAC	4 cut(s)	469, 520, 1488, 1771
NsiI	ATGCAT	2 cut(s)	928, 1054
NspI	RCATGY	3 cut(s)	366, 926, 1481
PaeI	GCATGC	1 cut(s)	1481
PagI	TCATGA	2 cut(s)	22, 1534
PciI	ACATGT	1 cut(s)	362
PciSI	GCTCTTC	1 cut(s)	1068
PctI	GAATGC	3 cut(s)	926, 1052, 1098
PdmI	GAANNNNTTC	1 cut(s)	1286
PfeI	GAWTC	5 cut(s)	212, 310, 907, 1045, 1180
PflFI	GACNNNGTC	1 cut(s)	1769
PkrI	GCNGC	5 cut(s)	118, 167, 304, 1476, 1799
PleI	GAGTC	4 cut(s)	910, 1005, 1019, 1132
PpsI	GAGTC	4 cut(s)	910, 1005, 1019, 1132
Ppu21I	YACGTR	1 cut(s)	156
PpuMI	RGGWCCY	1 cut(s)	722
PscI	ACATGT	1 cut(s)	362
PshBI	ATTAAT	3 cut(s)	465, 933, 1683
Psp124BI	GAGCTC	1 cut(s)	1063
Psp5II	RGGWCCY	1 cut(s)	722
Psp6I	CCWGG	2 cut(s)	397, 718
PspGI	CCWGG	2 cut(s)	397, 718
PspN4I	GGNNCC	4 cut(s)	345, 396, 724, 967
PspPI	GGNCC	2 cut(s)	394, 722
PspPPI	RGGWCCY	1 cut(s)	722
PstNI	CAGNNNCTG	2 cut(s)	116, 524
PsuI	RGATCY	2 cut(s)	343, 1563
PsyI	GACNNNGTC	1 cut(s)	1769
PvuII	CAGCTG	1 cut(s)	1277
RsaI	GTAC	6 cut(s)	158, 984, 1331, 1524, 1691, 1747
RsaNI	GTAC	6 cut(s)	157, 983, 1330, 1523, 1690, 1746
RseI	CAYNNNNRTG	3 cut(s)	128, 852, 1460
SacI	GAGCTC	1 cut(s)	1063
SapI	GCTCTTC	1 cut(s)	1068
SatI	GCNGC	5 cut(s)	117, 166, 303, 1475, 1798
Sau3AI	GATC	6 cut(s)	99, 132, 184, 343, 742, 1563
Sau96I	GGNCC	2 cut(s)	394, 722
ScaI	AGTACT	1 cut(s)	1747
SchI	GAGTC	4 cut(s)	910, 1006, 1020, 1133
ScrFI	CCNGG	2 cut(s)	399, 720
SduI	GDGCHC	5 cut(s)	787, 882, 1063, 1141, 1633
SfaNI	GCATC	6 cut(s)	211, 247, 1037, 1384, 1644, 1701
SinI	GGWCC	2 cut(s)	394, 722
SmiMI	CAYNNNNRTG	3 cut(s)	128, 852, 1460
SmlI	CTYRAG	2 cut(s)	788, 1788
SmoI	CTYRAG	2 cut(s)	788, 1788
SphI	GCATGC	1 cut(s)	1481
SsiI	CCGC	4 cut(s)	165, 168, 958, 1726
SspMI	CTAG	1 cut(s)	1097
SstI	GAGCTC	1 cut(s)	1063
StyD4I	CCNGG	2 cut(s)	397, 718
StyI	CCWWGG	1 cut(s)	1087
TaaI	ACNGT	9 cut(s)	112, 182, 508, 526, 703, 1247, 1334, 1436, 1522
TaiI	ACGT	3 cut(s)	158, 248, 341
TaqI	TCGA	1 cut(s)	1673
TatI	WGTACW	3 cut(s)	1522, 1689, 1745
TauI	GCSGC	1 cut(s)	168
TfiI	GAWTC	5 cut(s)	212, 310, 907, 1045, 1180
TscAI	CASTG	4 cut(s)	117, 525, 1462, 1512
TseFI	GTSAC	4 cut(s)	469, 520, 1488, 1771
TseI	GCWGC	4 cut(s)	116, 302, 1474, 1797
Tsp45I	GTSAC	4 cut(s)	469, 520, 1488, 1771
TspDTI	ATGAA	8 cut(s)	11, 17, 698, 768, 887, 919, 1248, 1401
TspRI	CASTG	4 cut(s)	117, 525, 1462, 1512
Tth111I	GACNNNGTC	1 cut(s)	1769
VneI	GTGCAC	3 cut(s)	783, 1137, 1629
VpaK11BI	GGWCC	2 cut(s)	394, 722
VspI	ATTAAT	3 cut(s)	465, 933, 1683
XapI	RAATTY	3 cut(s)	40, 1022, 1315
XceI	RCATGY	3 cut(s)	366, 926, 1481
XmiI	GTMKAC	1 cut(s)	566
XmnI	GAANNNNTTC	1 cut(s)	1286
XspI	CTAG	1 cut(s)	1097
ZrmI	AGTACT	1 cut(s)	1747
Zsp2I	ATGCAT	2 cut(s)	928, 1054

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.