Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0017265

Physical Location & Seq

Genomic Coordinates Reverse (-)

35176069 .. 35177340

Gene Structure 1272 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1159200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGAGCACTGGACCCGAGGACCGACCGTCGGCCGCGGCTCCACCGCCACAGTCTCCCTCGCCGCCAGCTCCGATGGCGAACGATTCGCGGTAAAGTCAGCCGAGCTCTCCAGCTCGGCTCTTTTGCAGAAGGAGCAATGTTTTTTGTCCAAGCTTGGTTCCCCCCATATAGTCAAGTACTTGGGCTATAATGTCAGCATTGAAAACAACAAGCCTATGTACAATCTCTGCATGGAGTACGTACCCGGCGGTACGCTTTTCGACGCGATTAGGAGGCGGGGAGGCCGGCTAGAGGAGGCCGTCATTCAATTGTACACGCACCAGATTGTGCAAGGATTGGAGTATTTGCATGTCAATAAATTGGTACACTGTGACATCAAGAGCCAAAACATTTTGGTGACCGGAGAGACCGCCAAAATTGCCGATTTGGGTTGCGCTAAATTGGTACAAGGGGTTGAGGCATGTACTACTATTTCGGGTACGCCGGTGTTTATGGCGCCGGAGGTTGCCCGTGGCGAAGAGCAGGGGTTTGAGGCTGACATATGGGCTCTTGGGTGCACGATTATTGAAATGGCAACCGGTGGCGGGCCTTGGCCGGAGATTAATGATCCGGTTTCAGCTCTCTACAGAATTGGATTCTCCGGCGAGCTGCCGGAGATTCCAAGCCAGCTTTCGAGCAAGGGTAAGGATTTCTTAAGCAAGTGTTTGATGAAGGATCCAAGAGAGAGGTGGACAGCTAAGCAGCTCCTTGATCATCCATTTCTTGAACAGCATTTGGGGTCCAAGTTTAATACAGAGCAAGTAATGGGGTCTCCAATTAGTGTGTTGGATCAAGGCCTTTGGGATTCTTTTGAAGCGTCTGAAAGTCCAATGGAATTGCTATCTGTTGAAGGTGTTTGTTTGAATTCCCCAACAGAAAGGATCAAGAAACTGATTGGATTTTCGACATTTTCGAATGTACCCAACTGGGAATTTGATGAAAATTGGTGCACTGTTAGAAGCAACATCGATGAAGAAATCGAAAACTTTGTGGTTTCAAACAACAATGTTGTCTCAGATGAAGAAGTGTTTGCTGCAAATTCTTCAACTGTGGCTTCAATTGTTCTTGAAGAGATTGAGAGTATACACTGTGATGAGGATTTAGATTTTGAATTTATGATTAGTAACAGTACGACAGAAGTTTCTTTTGTATCAAACAATCTTGAAATTGAATCAGATCATCCAAATTTTGATTTTATTCACTCCCATTGTTTTGCGTCTATTTTGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009653 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0099120 GO:0099568 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

424

Amino Acids

46.81

Weight (kDa)

4.64

Isoelectric Point (pI)

50.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	6 - 255	3.4e-58	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	9 - 250	4.4e-33	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0017265)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g23120
malus_domestica	MD00G1159200.v1.1 MD11G1213700.v1.1
prunus_persica	Prupe.4G217600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0040771
rosa_laevigata	RLG00000034000
rosa_multiflora	Rmu_ssc0000274.1_g000002
rosa_rugosa	Rorug05G0187100
rosa_samantha	Rh5AG272900 Rh5BG278000 Rh5CG310000 Rh5DG285900
rosa_wichuraiana	Rw5G025660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	496
AccI	GTMKAC	1 cut(s)	1123
AccII	CGCG	3 cut(s)	36, 89, 266
AciI	CCGC	9 cut(s)	34, 36, 45, 63, 89, 249, 277, 411, 585
AclWI	GGATC	5 cut(s)	602, 710, 723, 837, 929
AcoI	YGGCCR	2 cut(s)	31, 593
AcsI	RAATTY	5 cut(s)	904, 971, 1078, 1151, 1225
AcyI	GRCGYC	1 cut(s)	497
AfiI	CCNNNNNNNGG	3 cut(s)	29, 292, 585
AflII	CTTAAG	1 cut(s)	694
AgeI	ACCGGT	1 cut(s)	578
AhdI	GACNNNNNGTC	1 cut(s)	26
AjuI	GAANNNNNNNTTGG	2 cut(s)	655, 687
AluBI	AGCT	9 cut(s)	69, 106, 114, 154, 620, 649, 670, 737, 745
AluI	AGCT	9 cut(s)	69, 106, 114, 154, 620, 649, 670, 737, 745
Alw21I	GWGCWC	4 cut(s)	9, 108, 560, 992
Alw26I	GTCTC	4 cut(s)	58, 401, 816, 1057
Alw44I	GTGCAC	2 cut(s)	556, 988
AlwI	GGATC	5 cut(s)	602, 710, 723, 837, 929
Ama87I	CYCGRG	1 cut(s)	15
AoxI	GGCC	6 cut(s)	31, 283, 297, 587, 593, 835
ApaLI	GTGCAC	2 cut(s)	556, 988
ApeKI	GCWGC	3 cut(s)	649, 742, 1073
ApoI	RAATTY	5 cut(s)	904, 971, 1078, 1151, 1225
AseI	ATTAAT	1 cut(s)	603
AsiGI	ACCGGT	1 cut(s)	578
AspLEI	GCGC	2 cut(s)	437, 499
AspS9I	GGNCC	4 cut(s)	12, 20, 587, 780
AsuC2I	CCSGG	1 cut(s)	246
AsuHPI	GGTGA	1 cut(s)	409
AsuII	TTCGAA	1 cut(s)	953
AvaI	CYCGRG	1 cut(s)	15
AvaII	GGWCC	3 cut(s)	12, 20, 780
BaeGI	GKGCMC	2 cut(s)	560, 992
BaeI	ACNNNNGTAYC	2 cut(s)	471, 504
BamHI	GGATCC	1 cut(s)	715
BanI	GGYRCC	1 cut(s)	496
BanII	GRGCYC	2 cut(s)	108, 550
Bbv12I	GWGCWC	4 cut(s)	9, 108, 560, 992
BbvI	GCAGC	3 cut(s)	636, 754, 1060
BccI	CCATC	1 cut(s)	68
BceAI	ACGGC	1 cut(s)	284
BclI	TGATCA	1 cut(s)	751
BcnI	CCSGG	1 cut(s)	246
BcoDI	GTCTC	4 cut(s)	58, 401, 816, 1057
BfaI	CTAG	1 cut(s)	290
BfmI	CTRYAG	1 cut(s)	625
BfoI	RGCGCY	1 cut(s)	500
BfrI	CTTAAG	1 cut(s)	694
BisI	GCNGC	6 cut(s)	34, 37, 63, 650, 743, 1074
BlpI	GCTNAGC	1 cut(s)	738
BlsI	GCNGC	6 cut(s)	35, 38, 64, 651, 744, 1075
BmcAI	AGTACT	1 cut(s)	179
Bme1390I	CCNGG	1 cut(s)	246
Bme18I	GGWCC	3 cut(s)	12, 20, 780
BmeRI	GACNNNNNGTC	1 cut(s)	26
BmeT110I	CYCGRG	1 cut(s)	15
BmgT120I	GGNCC	4 cut(s)	12, 20, 587, 780
BmiI	GGNNCC	6 cut(s)	14, 40, 160, 498, 717, 781
BmrFI	CCNGG	1 cut(s)	246
BmrI	ACTGGG	1 cut(s)	976
BmuI	ACTGGG	1 cut(s)	976
BpmI	CTGGAG	1 cut(s)	94
Bpu1102I	GCTNAGC	1 cut(s)	738
Bpu14I	TTCGAA	1 cut(s)	953
BpuMI	CCSGG	1 cut(s)	246
Bsa29I	ATCGAT	1 cut(s)	1008
BsaAI	YACGTR	1 cut(s)	241
BsaHI	GRCGYC	1 cut(s)	497
BsaI	GGTCTC	2 cut(s)	401, 816
BsaJI	CCNNGG	4 cut(s)	16, 34, 511, 590
BsaWI	WCCGGW	3 cut(s)	401, 578, 610
Bsc4I	CCNNNNNNNGG	3 cut(s)	29, 292, 585
Bse118I	RCCGGY	3 cut(s)	285, 484, 578
Bse1I	ACTGG	2 cut(s)	14, 971
Bse3DI	GCAATG	1 cut(s)	143
BseCI	ATCGAT	1 cut(s)	1008
BseDI	CCNNGG	4 cut(s)	16, 34, 511, 590
BseGI	GGATG	2 cut(s)	754, 1219
BseLI	CCNNNNNNNGG	3 cut(s)	29, 292, 585
BseMI	GCAATG	1 cut(s)	143
BseMII	CTCAG	1 cut(s)	1068
BseNI	ACTGG	2 cut(s)	14, 971
BseRI	GAGGAG	1 cut(s)	308
BseSI	GKGCMC	2 cut(s)	560, 992
BseX3I	CGGCCG	1 cut(s)	31
BseXI	GCAGC	3 cut(s)	636, 754, 1060
Bsh1236I	CGCG	3 cut(s)	36, 89, 266
Bsh1285I	CGRYCG	2 cut(s)	27, 34
BshFI	GGCC	6 cut(s)	33, 285, 299, 589, 595, 837
BshNI	GGYRCC	1 cut(s)	496
BshTI	ACCGGT	1 cut(s)	578
BshVI	ATCGAT	1 cut(s)	1008
BsiEI	CGRYCG	2 cut(s)	27, 34
BsiHKAI	GWGCWC	4 cut(s)	9, 108, 560, 992
BsiHKCI	CYCGRG	1 cut(s)	15
BslI	CCNNNNNNNGG	3 cut(s)	29, 292, 585
BsmAI	GTCTC	4 cut(s)	58, 401, 816, 1057
BsnI	GGCC	6 cut(s)	33, 285, 299, 589, 595, 837
Bso31I	GGTCTC	2 cut(s)	401, 816
BsoBI	CYCGRG	1 cut(s)	15
Bsp119I	TTCGAA	1 cut(s)	953
Bsp1286I	GDGCHC	5 cut(s)	9, 108, 550, 560, 992
Bsp1407I	TGTACA	2 cut(s)	219, 312
Bsp143I	GATC	6 cut(s)	607, 715, 751, 829, 921, 1216
Bsp1720I	GCTNAGC	1 cut(s)	738
BspACI	CCGC	9 cut(s)	34, 36, 45, 63, 89, 249, 277, 411, 585
BspANI	GGCC	6 cut(s)	33, 285, 299, 589, 595, 837
BspCNI	CTCAG	1 cut(s)	1067
BspDI	ATCGAT	1 cut(s)	1008
BspFNI	CGCG	3 cut(s)	36, 89, 266
BspLI	GGNNCC	6 cut(s)	14, 40, 160, 498, 717, 781
BspPI	GGATC	5 cut(s)	602, 710, 723, 837, 929
BspQI	GCTCTTC	1 cut(s)	513
BspT104I	TTCGAA	1 cut(s)	953
BspT107I	GGYRCC	1 cut(s)	496
BspTI	CTTAAG	1 cut(s)	694
BspTNI	GGTCTC	2 cut(s)	401, 816
BsrDI	GCAATG	1 cut(s)	143
BsrFI	RCCGGY	3 cut(s)	285, 484, 578
BsrGI	TGTACA	2 cut(s)	219, 312
BsrI	ACTGG	2 cut(s)	14, 971
BssAI	RCCGGY	3 cut(s)	285, 484, 578
BssECI	CCNNGG	4 cut(s)	16, 34, 511, 590
BssMI	GATC	6 cut(s)	607, 715, 751, 829, 921, 1216
BssNAI	GTATAC	1 cut(s)	1124
BssNI	GRCGYC	1 cut(s)	497
BssT1I	CCWWGG	1 cut(s)	590
Bst1107I	GTATAC	1 cut(s)	1124
Bst4CI	ACNGT	7 cut(s)	28, 52, 371, 994, 1090, 1130, 1169
Bst6I	CTCTTC	2 cut(s)	513, 1104
BstACI	GRCGYC	1 cut(s)	497
BstAFI	CTTAAG	1 cut(s)	694
BstAUI	TGTACA	2 cut(s)	219, 312
BstBAI	YACGTR	1 cut(s)	241
BstBI	TTCGAA	1 cut(s)	953
BstC8I	GCNNGC	5 cut(s)	67, 287, 587, 647, 668
BstDEI	CTNAG	2 cut(s)	738, 1054
BstDSI	CCRYGG	2 cut(s)	34, 511
BstEII	GGTNACC	1 cut(s)	397
BstF5I	GGATG	2 cut(s)	754, 1219
BstFNI	CGCG	3 cut(s)	36, 89, 266
BstH2I	RGCGCY	1 cut(s)	500
BstHHI	GCGC	2 cut(s)	437, 499
BstKTI	GATC	6 cut(s)	610, 718, 754, 832, 924, 1219
BstMAI	GTCTC	4 cut(s)	58, 401, 816, 1057
BstMBI	GATC	6 cut(s)	607, 715, 751, 829, 921, 1216
BstMCI	CGRYCG	2 cut(s)	27, 34
BstMWI	GCNNNNNNNGC	3 cut(s)	75, 133, 419
BstNSI	RCATGY	2 cut(s)	353, 465
BstPI	GGTNACC	1 cut(s)	397
BstSCI	CCNGG	1 cut(s)	244
BstSFI	CTRYAG	1 cut(s)	625
BstSLI	GKGCMC	2 cut(s)	560, 992
BstSNI	TACGTA	1 cut(s)	241
BstUI	CGCG	3 cut(s)	36, 89, 266
BstV1I	GCAGC	3 cut(s)	636, 754, 1060
BstX2I	RGATCY	1 cut(s)	715
BstYI	RGATCY	1 cut(s)	715
BstZ17I	GTATAC	1 cut(s)	1124
BstZI	CGGCCG	1 cut(s)	31
Bsu15I	ATCGAT	1 cut(s)	1008
BsuRI	GGCC	6 cut(s)	33, 285, 299, 589, 595, 837
BsuTUI	ATCGAT	1 cut(s)	1008
BtgI	CCRYGG	2 cut(s)	34, 511
BtsCI	GGATG	2 cut(s)	754, 1219
BtsIMutI	CAGTG	4 cut(s)	7, 367, 990, 1126
Cac8I	GCNNGC	5 cut(s)	67, 287, 587, 647, 668
CfoI	GCGC	2 cut(s)	437, 499
Cfr10I	RCCGGY	3 cut(s)	285, 484, 578
Cfr13I	GGNCC	4 cut(s)	12, 20, 587, 780
Cfr42I	CCGCGG	1 cut(s)	37
ClaI	ATCGAT	1 cut(s)	1008
CseI	GACGC	3 cut(s)	272, 846, 1245
CspAI	ACCGGT	1 cut(s)	578
CviAII	CATG	3 cut(s)	232, 350, 462
DdeI	CTNAG	2 cut(s)	738, 1054
DinI	GGCGCC	1 cut(s)	498
DpnI	GATC	6 cut(s)	609, 717, 753, 831, 923, 1218
DpnII	GATC	6 cut(s)	607, 715, 751, 829, 921, 1216
DriI	GACNNNNNGTC	1 cut(s)	26
EaeI	YGGCCR	2 cut(s)	31, 593
EagI	CGGCCG	1 cut(s)	31
Eam1104I	CTCTTC	2 cut(s)	513, 1104
Eam1105I	GACNNNNNGTC	1 cut(s)	26
EarI	CTCTTC	2 cut(s)	513, 1104
Ecl136II	GAGCTC	1 cut(s)	106
EclXI	CGGCCG	1 cut(s)	31
Eco105I	TACGTA	1 cut(s)	241
Eco130I	CCWWGG	1 cut(s)	590
Eco147I	AGGCCT	1 cut(s)	837
Eco24I	GRGCYC	2 cut(s)	108, 550
Eco31I	GGTCTC	2 cut(s)	401, 816
Eco47I	GGWCC	3 cut(s)	12, 20, 780
Eco52I	CGGCCG	1 cut(s)	31
Eco53kI	GAGCTC	1 cut(s)	106
Eco88I	CYCGRG	1 cut(s)	15
Eco91I	GGTNACC	1 cut(s)	397
EcoICRI	GAGCTC	1 cut(s)	106
EcoO65I	GGTNACC	1 cut(s)	397
EcoRI	GAATTC	1 cut(s)	904
EcoT14I	CCWWGG	1 cut(s)	590
EcoT38I	GRGCYC	2 cut(s)	108, 550
EgeI	GGCGCC	1 cut(s)	498
EheI	GGCGCC	1 cut(s)	498
ErhI	CCWWGG	1 cut(s)	590
FaeI	CATG	3 cut(s)	235, 353, 465
FalI	AAGNNNNNCTT	2 cut(s)	677, 709
FatI	CATG	3 cut(s)	231, 349, 461
FauI	CCCGC	2 cut(s)	270, 578
FauNDI	CATATG	1 cut(s)	542
FbaI	TGATCA	1 cut(s)	751
FblI	GTMKAC	1 cut(s)	1123
Fnu4HI	GCNGC	6 cut(s)	34, 37, 63, 650, 743, 1074
FokI	GGATG	2 cut(s)	741, 1206
FriOI	GRGCYC	2 cut(s)	108, 550
Fsp4HI	GCNGC	6 cut(s)	34, 37, 63, 650, 743, 1074
FspBI	CTAG	1 cut(s)	290
GlaI	GCGC	2 cut(s)	436, 498
GluI	GCNGC	6 cut(s)	34, 37, 63, 650, 743, 1074
GsuI	CTGGAG	1 cut(s)	94
HaeII	RGCGCY	1 cut(s)	500
HaeIII	GGCC	6 cut(s)	33, 285, 299, 589, 595, 837
HgaI	GACGC	3 cut(s)	272, 846, 1245
HhaI	GCGC	2 cut(s)	437, 499
Hin1I	GRCGYC	1 cut(s)	497
Hin1II	CATG	3 cut(s)	235, 353, 465
Hin6I	GCGC	2 cut(s)	435, 497
HinP1I	GCGC	2 cut(s)	435, 497
HindIII	AAGCTT	1 cut(s)	152
HinfI	GANTC	5 cut(s)	84, 636, 658, 845, 1211
HphI	GGTGA	1 cut(s)	409
Hpy166II	GTNNAC	6 cut(s)	315, 367, 558, 732, 990, 1124
Hpy188I	TCNGA	4 cut(s)	73, 862, 1057, 1216
Hpy188III	TCNNGA	5 cut(s)	379, 764, 925, 1106, 1202
Hpy8I	GTNNAC	6 cut(s)	315, 367, 558, 732, 990, 1124
Hpy99I	CGWCG	2 cut(s)	32, 266
HpyAV	CCTTC	3 cut(s)	124, 706, 884
HpyCH4III	ACNGT	7 cut(s)	28, 52, 371, 994, 1090, 1130, 1169
HpyCH4IV	ACGT	1 cut(s)	240
HpyCH4V	TGCA	7 cut(s)	127, 231, 331, 349, 558, 990, 1076
HpyF10VI	GCNNNNNNNGC	3 cut(s)	75, 133, 419
HpyF3I	CTNAG	2 cut(s)	738, 1054
HpySE526I	ACGT	1 cut(s)	240
Hsp92I	GRCGYC	1 cut(s)	497
Hsp92II	CATG	3 cut(s)	235, 353, 465
HspAI	GCGC	2 cut(s)	435, 497
KasI	GGCGCC	1 cut(s)	496
KroI	GCCGGC	1 cut(s)	285
KroNI	GCCGGC	1 cut(s)	287
Ksp22I	TGATCA	1 cut(s)	751
KspI	CCGCGG	1 cut(s)	37
Kzo9I	GATC	6 cut(s)	607, 715, 751, 829, 921, 1216
LguI	GCTCTTC	1 cut(s)	513
LmnI	GCTCC	5 cut(s)	4, 44, 74, 133, 750
Lsp1109I	GCAGC	3 cut(s)	636, 754, 1060
MaeI	CTAG	1 cut(s)	290
MaeII	ACGT	1 cut(s)	240
MaeIII	GTNAC	3 cut(s)	371, 397, 1163
MalI	GATC	6 cut(s)	609, 717, 753, 831, 923, 1218
MboI	GATC	6 cut(s)	607, 715, 751, 829, 921, 1216
MboII	GAAGA	5 cut(s)	530, 1025, 1073, 1074, 1121
MfeI	CAATTG	2 cut(s)	308, 1098
MflI	RGATCY	1 cut(s)	715
MhlI	GDGCHC	5 cut(s)	9, 108, 550, 560, 992
Mly113I	GGCGCC	1 cut(s)	497
MmeI	TCCRAC	1 cut(s)	807
MroNI	GCCGGC	1 cut(s)	285
MseI	TTAA	3 cut(s)	603, 695, 789
MslI	CAYNNNNRTG	2 cut(s)	395, 1131
MspA1I	CMGCKG	1 cut(s)	36
MspCI	CTTAAG	1 cut(s)	694
MspR9I	CCNGG	1 cut(s)	246
MunI	CAATTG	2 cut(s)	308, 1098
MvnI	CGCG	3 cut(s)	36, 89, 266
MwoI	GCNNNNNNNGC	3 cut(s)	75, 133, 419
NaeI	GCCGGC	1 cut(s)	287
NarI	GGCGCC	1 cut(s)	497
NciI	CCSGG	1 cut(s)	246
NdeI	CATATG	1 cut(s)	542
NdeII	GATC	6 cut(s)	607, 715, 751, 829, 921, 1216
NgoMIV	GCCGGC	1 cut(s)	285
NlaIII	CATG	3 cut(s)	235, 353, 465
NlaIV	GGNNCC	6 cut(s)	14, 40, 160, 498, 717, 781
NmeAIII	GCCGAG	2 cut(s)	95, 127
NmuCI	GTSAC	2 cut(s)	371, 397
NspI	RCATGY	2 cut(s)	353, 465
NspV	TTCGAA	1 cut(s)	953
PceI	AGGCCT	1 cut(s)	837
PciSI	GCTCTTC	1 cut(s)	513
PcsI	WCGNNNNNNNCGW	1 cut(s)	950
PdiI	GCCGGC	1 cut(s)	287
PfeI	GAWTC	5 cut(s)	84, 636, 658, 845, 1211
PinAI	ACCGGT	1 cut(s)	578
PkrI	GCNGC	6 cut(s)	35, 38, 64, 651, 744, 1075
PluTI	GGCGCC	1 cut(s)	500
Ppu21I	YACGTR	1 cut(s)	241
PshBI	ATTAAT	1 cut(s)	603
Psp124BI	GAGCTC	1 cut(s)	108
PspEI	GGTNACC	1 cut(s)	397
PspN4I	GGNNCC	6 cut(s)	14, 40, 160, 498, 717, 781
PspPI	GGNCC	4 cut(s)	12, 20, 587, 780
PsuI	RGATCY	1 cut(s)	715
RseI	CAYNNNNRTG	2 cut(s)	395, 1131
SacI	GAGCTC	1 cut(s)	108
SacII	CCGCGG	1 cut(s)	37
SapI	GCTCTTC	1 cut(s)	513
SaqAI	TTAA	3 cut(s)	603, 695, 789
SatI	GCNGC	6 cut(s)	34, 37, 63, 650, 743, 1074
Sau3AI	GATC	6 cut(s)	607, 715, 751, 829, 921, 1216
Sau96I	GGNCC	4 cut(s)	12, 20, 587, 780
ScaI	AGTACT	1 cut(s)	179
ScrFI	CCNGG	1 cut(s)	246
SduI	GDGCHC	5 cut(s)	9, 108, 550, 560, 992
SfcI	CTRYAG	1 cut(s)	625
SfoI	GGCGCC	1 cut(s)	498
Sfr303I	CCGCGG	1 cut(s)	37
SfuI	TTCGAA	1 cut(s)	953
SgrAI	CRCCGGYG	1 cut(s)	484
SgrBI	CCGCGG	1 cut(s)	37
SinI	GGWCC	3 cut(s)	12, 20, 780
SmiMI	CAYNNNNRTG	2 cut(s)	395, 1131
SmlI	CTYRAG	1 cut(s)	694
SmoI	CTYRAG	1 cut(s)	694
SnaBI	TACGTA	1 cut(s)	241
SseBI	AGGCCT	1 cut(s)	837
SsiI	CCGC	9 cut(s)	34, 36, 45, 63, 89, 249, 277, 411, 585
SspDI	GGCGCC	1 cut(s)	496
SspMI	CTAG	1 cut(s)	290
SstI	GAGCTC	1 cut(s)	108
StuI	AGGCCT	1 cut(s)	837
StyD4I	CCNGG	1 cut(s)	244
StyI	CCWWGG	1 cut(s)	590
TaaI	ACNGT	7 cut(s)	28, 52, 371, 994, 1090, 1130, 1169
TaiI	ACGT	1 cut(s)	243
TaqI	TCGA	6 cut(s)	261, 674, 944, 953, 1008, 1020
TaqII	GACCGA	1 cut(s)	37
TatI	WGTACW	4 cut(s)	177, 219, 312, 464
TauI	GCSGC	3 cut(s)	36, 39, 65
TfiI	GAWTC	5 cut(s)	84, 636, 658, 845, 1211
Tru1I	TTAA	3 cut(s)	603, 695, 789
Tru9I	TTAA	3 cut(s)	603, 695, 789
TscAI	CASTG	4 cut(s)	14, 374, 997, 1133
TseFI	GTSAC	2 cut(s)	371, 397
TseI	GCWGC	3 cut(s)	649, 742, 1073
Tsp45I	GTSAC	2 cut(s)	371, 397
TspDTI	ATGAA	4 cut(s)	725, 993, 1026, 1074
TspRI	CASTG	4 cut(s)	14, 374, 997, 1133
Vha464I	CTTAAG	1 cut(s)	694
VneI	GTGCAC	2 cut(s)	556, 988
VpaK11BI	GGWCC	3 cut(s)	12, 20, 780
VspI	ATTAAT	1 cut(s)	603
XapI	RAATTY	5 cut(s)	904, 971, 1078, 1151, 1225
XceI	RCATGY	2 cut(s)	353, 465
XcmI	CCANNNNNNNNNTGG	1 cut(s)	726
XmiI	GTMKAC	1 cut(s)	1123
XspI	CTAG	1 cut(s)	290
ZrmI	AGTACT	1 cut(s)	179

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.