Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0017265

Physical Location & Seq

Genomic Coordinates Reverse (-)

34237123 .. 34239148

Gene Structure 2026 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG310000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGTGATGGAGTGGACTAGAGGACCAACGATCGGCCGCGGCTCCACCGCCACCGTCTCTCTAGCCACATCAGCCCTCTCCGGTGAGGTCTTCGCGGTGAAGTCGGCCGAGCTTTCGCAGTCGTCGTTCTTGCAAAAGGAGCAGTGTTTTCTATCTAAGCTGAGCTCTCCTCATGTGGTCAAGTACTTAGGTTGTGATGTTAGCACTGAACACAATGAGCCTACGTACAATCTTTTCATGGAGTACGTACCCGGCGGCACGCTTTCCGATGCGATTCGAAGACATGGAAAGGAGCTCGAGGAGTCTATGATCCAATCATACACGCACCAGATTGTGGAGGGGTTGGGATATCTTCATTCAAATGGGTTGGCACATTGTGACATTAAGAGCCAGAACATTTTGGTGTCTGAAGAGAGTGCCAAGATCAAAATTGCTGATCTGGGTTGTGCTAAATTGGTGCATGAAGCATCCACGTCGGCATTTTCCGGCACGCCGGTGTTCATGGCGCCGGAGGTGGCACGCGGAGAAGAGCAGGGTTTTGAAGCAGATATATGGGCACTTGGCTGTACATTGATTGAAATGGCCACAGGGGCCAATAGCCCCTGGCCGGATGTAAGTGAACCTGTTTCGGCTCTCTACCGGATTGGATTCTCCGGTGACTTGCCGGAGTTTCCGAGGTGGCTTTCAAAAGAGGGTAAGGATTTCTTGAGCAAGTGTTTGACGACATGCCCAAGAGAGAGGTGGACAGCTAAGAAGCTTCTTGAGCATCCATTTCTCAAAGACTACTCAATGGGGTCTACGAGTTTGAGCTCATCTCCTATTAGTGTGTTGGATCAAGGCCTTTGGGATTCATTTGAAGTCTTTGAAGAAGCTCCTCAGAATCAAGTTTGTGAAAGCATTTGTTCCAGTTCTTCTCCGCAAGAAAGGATCAAGATGGTGATCGGAGGTACTTGTTCTACATTTTCAGGTATACCCAATTGGGAATTTGATGAGAATTGGTGCACTGTTAGAAGCAATGGTGCCGAGGATAGCGAAGCCTTATTGAAGAACAACAATGTCATTGTTGCTACTTCAGAGCAGGAATATATGGCGGCAACTGTTACATCAGATATGGATTCCATTATTGATGAGGAGCTTCACAGTTCAATGTGTGGTGATGATGAAGATGATGATGAGGAATTAGATTCTGAATGTTTTGTAGAGTCTAACAGTAGAATTGGTAGTGAAGGAGATTTTGTAATTTCATACATTGTTACAGACGATTCTTTCGTAAACAATCTTGATATTGAACAAGATACTCGGAATTATTCTTTTATTCACTCCCATTGTTATCATTCGTGTTTTGATTTTTCTGGTCAATCTGTGGTGCTTAAGTTCCTCTTGTTAATGTCTTCTCTGTTTTGTGTTAGTTTGGTACAATGCCACATGACAGATCAATTTCTCTACGGGGTAAATGCATATATATATATGTTAAGGGCCAAATGCCTTATTCGAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009653 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0099120 GO:0099568 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

499

Amino Acids

55.25

Weight (kDa)

4.55

Isoelectric Point (pI)

49.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	7 - 259	1.3e-57	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	10 - 255	4.8e-35	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0017265)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g23120
malus_domestica	MD00G1159200.v1.1 MD11G1213700.v1.1
prunus_persica	Prupe.4G217600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0040771
rosa_laevigata	RLG00000034000
rosa_multiflora	Rmu_ssc0000274.1_g000002
rosa_rugosa	Rorug05G0187100
rosa_samantha	Rh5AG272900 Rh5BG278000 Rh5CG310000 Rh5DG285900
rosa_wichuraiana	Rw5G025660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	505, 1019
AccB7I	CCANNNNNTGG	1 cut(s)	334
AccI	GTMKAC	2 cut(s)	797, 970
AccII	CGCG	3 cut(s)	39, 95, 522
AciI	CCGC	8 cut(s)	37, 39, 48, 95, 255, 522, 917, 1091
AclWI	GGATC	3 cut(s)	304, 840, 935
AcoI	YGGCCR	4 cut(s)	34, 105, 582, 605
AcsI	RAATTY	1 cut(s)	983
AcuI	CTGAAG	2 cut(s)	429, 1056
AcyI	GRCGYC	1 cut(s)	506
AdeI	CACNNNGTG	1 cut(s)	377
AfaI	GTAC	7 cut(s)	185, 227, 245, 249, 568, 949, 1416
AfiI	CCNNNNNNNGG	3 cut(s)	32, 334, 607
AflII	CTTAAG	1 cut(s)	1370
AgsI	TTSAA	9 cut(s)	360, 542, 578, 687, 857, 866, 1045, 1146, 1289
AjiI	CACGTC	1 cut(s)	474
AjnI	CCWGG	1 cut(s)	602
AleI	CACNNNNGTG	1 cut(s)	494
AluBI	AGCT	9 cut(s)	112, 160, 165, 295, 749, 757, 810, 872, 1135
AluI	AGCT	9 cut(s)	112, 160, 165, 295, 749, 757, 810, 872, 1135
Alw21I	GWGCWC	4 cut(s)	167, 297, 812, 1004
Alw26I	GTCTC	1 cut(s)	61
Alw44I	GTGCAC	1 cut(s)	1000
AlwI	GGATC	3 cut(s)	304, 840, 935
Ama87I	CYCGRG	1 cut(s)	296
AoxI	GGCC	7 cut(s)	34, 105, 582, 591, 605, 838, 1476
ApaLI	GTGCAC	1 cut(s)	1000
ApoI	RAATTY	1 cut(s)	983
ArsI	GACNNNNNNTTYG	2 cut(s)	1250, 1282
AspLEI	GCGC	1 cut(s)	508
AspS9I	GGNCC	3 cut(s)	23, 591, 1476
AsuC2I	CCSGG	1 cut(s)	252
AsuHPI	GGTGA	6 cut(s)	16, 95, 109, 668, 949, 1166
AsuII	TTCGAA	1 cut(s)	277
AvaI	CYCGRG	1 cut(s)	296
AvaII	GGWCC	1 cut(s)	23
BaeGI	GKGCMC	2 cut(s)	559, 1004
BalI	TGGCCA	1 cut(s)	584
BanI	GGYRCC	2 cut(s)	505, 1019
BanII	GRGCYC	3 cut(s)	167, 297, 812
BbsI	GAAGAC	3 cut(s)	82, 286, 1383
Bbv12I	GWGCWC	4 cut(s)	167, 297, 812, 1004
BccI	CCATC	1 cut(s)	928
BcgI	CGANNNNNNTGC	2 cut(s)	456, 490
BciT130I	CCWGG	1 cut(s)	604
BcnI	CCSGG	1 cut(s)	252
BcoDI	GTCTC	1 cut(s)	61
BfaI	CTAG	2 cut(s)	18, 62
BfoI	RGCGCY	1 cut(s)	509
BfrI	CTTAAG	1 cut(s)	1370
BglI	GCCNNNNNGGC	1 cut(s)	590
BisI	GCNGC	4 cut(s)	37, 40, 256, 1092
BlpI	GCTNAGC	1 cut(s)	161
BlsI	GCNGC	4 cut(s)	38, 41, 257, 1093
BmcAI	AGTACT	1 cut(s)	185
Bme1390I	CCNGG	2 cut(s)	252, 604
Bme18I	GGWCC	1 cut(s)	23
BmeT110I	CYCGRG	1 cut(s)	296
BmgBI	CACGTC	1 cut(s)	474
BmgT120I	GGNCC	3 cut(s)	23, 591, 1476
BmiI	GGNNCC	4 cut(s)	43, 507, 592, 1021
BmrFI	CCNGG	2 cut(s)	252, 604
BmsI	GCATC	3 cut(s)	259, 476, 775
BpiI	GAAGAC	3 cut(s)	82, 286, 1383
BplI	GAGNNNNNCTC	4 cut(s)	154, 186, 799, 831
Bpu1102I	GCTNAGC	1 cut(s)	161
Bpu14I	TTCGAA	1 cut(s)	277
BpuEI	CTTGAG	2 cut(s)	727, 782
BpuMI	CCSGG	1 cut(s)	252
BsaAI	YACGTR	2 cut(s)	225, 247
BsaBI	GATNNNNATC	1 cut(s)	938
BsaHI	GRCGYC	1 cut(s)	506
BsaJI	CCNNGG	4 cut(s)	37, 602, 674, 1023
BsaWI	WCCGGW	3 cut(s)	80, 639, 653
BsaXI	ACNNNNNCTCC	2 cut(s)	1221, 1251
Bsc4I	CCNNNNNNNGG	3 cut(s)	32, 334, 607
Bse118I	RCCGGY	1 cut(s)	493
Bse1I	ACTGG	1 cut(s)	906
Bse3DI	GCAATG	1 cut(s)	1021
Bse8I	GATNNNNATC	1 cut(s)	938
BseBI	CCWGG	1 cut(s)	604
BseDI	CCNNGG	4 cut(s)	37, 602, 674, 1023
BseGI	GGATG	3 cut(s)	467, 616, 766
BseJI	GATNNNNATC	1 cut(s)	938
BseLI	CCNNNNNNNGG	3 cut(s)	32, 334, 607
BseMI	GCAATG	1 cut(s)	1021
BseMII	CTCAG	2 cut(s)	152, 890
BseNI	ACTGG	1 cut(s)	906
BseRI	GAGGAG	4 cut(s)	159, 314, 864, 1145
BseSI	GKGCMC	2 cut(s)	559, 1004
BseX3I	CGGCCG	2 cut(s)	34, 105
Bsh1236I	CGCG	3 cut(s)	39, 95, 522
Bsh1285I	CGRYCG	3 cut(s)	33, 37, 108
BshFI	GGCC	7 cut(s)	36, 107, 584, 593, 607, 840, 1478
BshNI	GGYRCC	2 cut(s)	505, 1019
BsiEI	CGRYCG	3 cut(s)	33, 37, 108
BsiHKAI	GWGCWC	4 cut(s)	167, 297, 812, 1004
BsiHKCI	CYCGRG	1 cut(s)	296
BsiSI	CCGG	9 cut(s)	81, 252, 486, 494, 509, 608, 640, 654, 665
BslI	CCNNNNNNNGG	3 cut(s)	32, 334, 607
BsmAI	GTCTC	1 cut(s)	61
BsmBI	CGTCTC	1 cut(s)	61
BsnI	GGCC	7 cut(s)	36, 107, 584, 593, 607, 840, 1478
BsoBI	CYCGRG	1 cut(s)	296
Bsp119I	TTCGAA	1 cut(s)	277
Bsp1286I	GDGCHC	5 cut(s)	167, 297, 559, 812, 1004
Bsp1407I	TGTACA	1 cut(s)	566
Bsp143I	GATC	8 cut(s)	30, 309, 423, 436, 832, 927, 939, 1432
Bsp1720I	GCTNAGC	1 cut(s)	161
BspACI	CCGC	8 cut(s)	37, 39, 48, 95, 255, 522, 917, 1091
BspANI	GGCC	7 cut(s)	36, 107, 584, 593, 607, 840, 1478
BspCNI	CTCAG	2 cut(s)	153, 889
BspFNI	CGCG	3 cut(s)	39, 95, 522
BspLI	GGNNCC	4 cut(s)	43, 507, 592, 1021
BspPI	GGATC	3 cut(s)	304, 840, 935
BspQI	GCTCTTC	1 cut(s)	522
BspT104I	TTCGAA	1 cut(s)	277
BspT107I	GGYRCC	2 cut(s)	505, 1019
BspTI	CTTAAG	1 cut(s)	1370
BsrDI	GCAATG	1 cut(s)	1021
BsrFI	RCCGGY	1 cut(s)	493
BsrGI	TGTACA	1 cut(s)	566
BsrI	ACTGG	1 cut(s)	906
BssAI	RCCGGY	1 cut(s)	493
BssECI	CCNNGG	4 cut(s)	37, 602, 674, 1023
BssMI	GATC	8 cut(s)	30, 309, 423, 436, 832, 927, 939, 1432
BssNAI	GTATAC	1 cut(s)	971
BssNI	GRCGYC	1 cut(s)	506
Bst1107I	GTATAC	1 cut(s)	971
Bst2UI	CCWGG	1 cut(s)	604
Bst4CI	ACNGT	5 cut(s)	55, 1006, 1099, 1142, 1211
Bst6I	CTCTTC	2 cut(s)	405, 522
BstACI	GRCGYC	1 cut(s)	506
BstAFI	CTTAAG	1 cut(s)	1370
BstAUI	TGTACA	1 cut(s)	566
BstBAI	YACGTR	2 cut(s)	225, 247
BstBI	TTCGAA	1 cut(s)	277
BstC8I	GCNNGC	3 cut(s)	260, 491, 520
BstDEI	CTNAG	5 cut(s)	156, 161, 187, 750, 876
BstDSI	CCRYGG	1 cut(s)	37
BstF5I	GGATG	3 cut(s)	467, 616, 766
BstFNI	CGCG	3 cut(s)	39, 95, 522
BstH2I	RGCGCY	1 cut(s)	509
BstHHI	GCGC	1 cut(s)	508
BstKTI	GATC	8 cut(s)	33, 312, 426, 439, 835, 930, 942, 1435
BstMAI	GTCTC	1 cut(s)	61
BstMBI	GATC	8 cut(s)	30, 309, 423, 436, 832, 927, 939, 1432
BstMCI	CGRYCG	3 cut(s)	33, 37, 108
BstMWI	GCNNNNNNNGC	4 cut(s)	71, 139, 590, 763
BstNI	CCWGG	1 cut(s)	604
BstNSI	RCATGY	1 cut(s)	729
BstSCI	CCNGG	2 cut(s)	250, 602
BstSLI	GKGCMC	2 cut(s)	559, 1004
BstSNI	TACGTA	2 cut(s)	225, 247
BstUI	CGCG	3 cut(s)	39, 95, 522
BstV2I	GAAGAC	3 cut(s)	82, 286, 1383
BstZ17I	GTATAC	1 cut(s)	971
BstZI	CGGCCG	2 cut(s)	34, 105
BsuRI	GGCC	7 cut(s)	36, 107, 584, 593, 607, 840, 1478
BtgI	CCRYGG	1 cut(s)	37
BtrI	CACGTC	1 cut(s)	474
BtsCI	GGATG	3 cut(s)	467, 616, 766
BtsI	GCAGTG	1 cut(s)	149
BtsIMutI	CAGTG	3 cut(s)	149, 204, 1002
Cac8I	GCNNGC	3 cut(s)	260, 491, 520
CfoI	GCGC	1 cut(s)	508
Cfr10I	RCCGGY	1 cut(s)	493
Cfr13I	GGNCC	3 cut(s)	23, 591, 1476
Cfr42I	CCGCGG	1 cut(s)	40
Csp6I	GTAC	7 cut(s)	184, 226, 244, 248, 567, 948, 1415
CviAII	CATG	7 cut(s)	173, 238, 284, 461, 502, 726, 1426
CviQI	GTAC	7 cut(s)	184, 226, 244, 248, 567, 948, 1415
DdeI	CTNAG	5 cut(s)	156, 161, 187, 750, 876
DinI	GGCGCC	1 cut(s)	507
DpnI	GATC	8 cut(s)	32, 311, 425, 438, 834, 929, 941, 1434
DpnII	GATC	8 cut(s)	30, 309, 423, 436, 832, 927, 939, 1432
DraIII	CACNNNGTG	1 cut(s)	377
EaeI	YGGCCR	4 cut(s)	34, 105, 582, 605
EagI	CGGCCG	2 cut(s)	34, 105
Eam1104I	CTCTTC	2 cut(s)	405, 522
EarI	CTCTTC	2 cut(s)	405, 522
Ecl136II	GAGCTC	3 cut(s)	165, 295, 810
EclXI	CGGCCG	2 cut(s)	34, 105
Eco105I	TACGTA	2 cut(s)	225, 247
Eco147I	AGGCCT	1 cut(s)	840
Eco24I	GRGCYC	3 cut(s)	167, 297, 812
Eco32I	GATATC	1 cut(s)	350
Eco47I	GGWCC	1 cut(s)	23
Eco52I	CGGCCG	2 cut(s)	34, 105
Eco53kI	GAGCTC	3 cut(s)	165, 295, 810
Eco57I	CTGAAG	2 cut(s)	429, 1056
Eco88I	CYCGRG	1 cut(s)	296
EcoICRI	GAGCTC	3 cut(s)	165, 295, 810
EcoRII	CCWGG	1 cut(s)	602
EcoRV	GATATC	1 cut(s)	350
EcoT22I	ATGCAT	1 cut(s)	1459
EcoT38I	GRGCYC	3 cut(s)	167, 297, 812
EgeI	GGCGCC	1 cut(s)	507
EheI	GGCGCC	1 cut(s)	507
Esp3I	CGTCTC	1 cut(s)	61
FaeI	CATG	7 cut(s)	176, 241, 287, 464, 505, 729, 1429
FalI	AAGNNNNNCTT	4 cut(s)	689, 721, 1364, 1396
FatI	CATG	7 cut(s)	172, 237, 283, 460, 501, 725, 1425
FblI	GTMKAC	2 cut(s)	797, 970
Fnu4HI	GCNGC	4 cut(s)	37, 40, 256, 1092
FokI	GGATG	3 cut(s)	454, 623, 753
FriOI	GRGCYC	3 cut(s)	167, 297, 812
Fsp4HI	GCNGC	4 cut(s)	37, 40, 256, 1092
FspBI	CTAG	2 cut(s)	18, 62
GlaI	GCGC	1 cut(s)	507
GluI	GCNGC	4 cut(s)	37, 40, 256, 1092
HaeII	RGCGCY	1 cut(s)	509
HaeIII	GGCC	7 cut(s)	36, 107, 584, 593, 607, 840, 1478
HapII	CCGG	9 cut(s)	81, 252, 486, 494, 509, 608, 640, 654, 665
HhaI	GCGC	1 cut(s)	508
Hin1I	GRCGYC	1 cut(s)	506
Hin1II	CATG	7 cut(s)	176, 241, 287, 464, 505, 729, 1429
Hin6I	GCGC	1 cut(s)	506
HinP1I	GCGC	1 cut(s)	506
HindIII	AAGCTT	1 cut(s)	755
HinfI	GANTC	9 cut(s)	274, 302, 648, 848, 880, 1115, 1184, 1202, 1262
HpaII	CCGG	9 cut(s)	81, 252, 486, 494, 509, 608, 640, 654, 665
HphI	GGTGA	6 cut(s)	16, 95, 109, 668, 949, 1166
Hpy166II	GTNNAC	7 cut(s)	15, 620, 744, 798, 971, 1002, 1273
Hpy188I	TCNGA	9 cut(s)	268, 409, 675, 879, 944, 1075, 1108, 1189, 1302
Hpy188III	TCNNGA	4 cut(s)	706, 761, 931, 1280
Hpy8I	GTNNAC	7 cut(s)	15, 620, 744, 798, 971, 1002, 1273
Hpy99I	CGWCG	2 cut(s)	127, 478
HpyAV	CCTTC	1 cut(s)	1220
HpyCH4III	ACNGT	5 cut(s)	55, 1006, 1099, 1142, 1211
HpyCH4IV	ACGT	3 cut(s)	224, 246, 473
HpyCH4V	TGCA	4 cut(s)	133, 460, 1002, 1457
HpyF10VI	GCNNNNNNNGC	4 cut(s)	71, 139, 590, 763
HpyF3I	CTNAG	5 cut(s)	156, 161, 187, 750, 876
HpySE526I	ACGT	3 cut(s)	224, 246, 473
Hsp92I	GRCGYC	1 cut(s)	506
Hsp92II	CATG	7 cut(s)	176, 241, 287, 464, 505, 729, 1429
HspAI	GCGC	1 cut(s)	506
KasI	GGCGCC	1 cut(s)	505
KspI	CCGCGG	1 cut(s)	40
Kzo9I	GATC	8 cut(s)	30, 309, 423, 436, 832, 927, 939, 1432
LguI	GCTCTTC	1 cut(s)	522
LmnI	GCTCC	5 cut(s)	47, 139, 292, 877, 1132
LweI	GCATC	3 cut(s)	259, 476, 775
MaeI	CTAG	2 cut(s)	18, 62
MaeII	ACGT	3 cut(s)	224, 246, 473
MaeIII	GTNAC	4 cut(s)	377, 656, 1099, 1252
MalI	GATC	8 cut(s)	32, 311, 425, 438, 834, 929, 941, 1434
MboI	GATC	8 cut(s)	30, 309, 423, 436, 832, 927, 939, 1432
MfeI	CAATTG	1 cut(s)	976
MhlI	GDGCHC	5 cut(s)	167, 297, 559, 812, 1004
MlsI	TGGCCA	1 cut(s)	584
MluNI	TGGCCA	1 cut(s)	584
Mly113I	GGCGCC	1 cut(s)	506
MlyI	GAGTC	2 cut(s)	311, 1211
MmeI	TCCRAC	1 cut(s)	810
Mox20I	TGGCCA	1 cut(s)	584
Mph1103I	ATGCAT	1 cut(s)	1459
MscI	TGGCCA	1 cut(s)	584
MseI	TTAA	4 cut(s)	384, 1371, 1385, 1472
MslI	CAYNNNNRTG	3 cut(s)	360, 401, 494
Msp20I	TGGCCA	1 cut(s)	584
MspA1I	CMGCKG	1 cut(s)	39
MspCI	CTTAAG	1 cut(s)	1370
MspI	CCGG	9 cut(s)	81, 252, 486, 494, 509, 608, 640, 654, 665
MspR9I	CCNGG	2 cut(s)	252, 604
MunI	CAATTG	1 cut(s)	976
MvaI	CCWGG	1 cut(s)	604
MvnI	CGCG	3 cut(s)	39, 95, 522
MwoI	GCNNNNNNNGC	4 cut(s)	71, 139, 590, 763
NarI	GGCGCC	1 cut(s)	506
NciI	CCSGG	1 cut(s)	252
NdeII	GATC	8 cut(s)	30, 309, 423, 436, 832, 927, 939, 1432
NlaIII	CATG	7 cut(s)	176, 241, 287, 464, 505, 729, 1429
NlaIV	GGNNCC	4 cut(s)	43, 507, 592, 1021
NmeAIII	GCCGAG	2 cut(s)	133, 1048
NmuCI	GTSAC	2 cut(s)	377, 656
NsiI	ATGCAT	1 cut(s)	1459
NspI	RCATGY	1 cut(s)	729
NspV	TTCGAA	1 cut(s)	277
OliI	CACNNNNGTG	1 cut(s)	494
PaeR7I	CTCGAG	1 cut(s)	296
PceI	AGGCCT	1 cut(s)	840
PciSI	GCTCTTC	1 cut(s)	522
PcsI	WCGNNNNNNNCGW	2 cut(s)	122, 1266
PfeI	GAWTC	7 cut(s)	274, 648, 848, 880, 1115, 1184, 1262
PflMI	CCANNNNNTGG	1 cut(s)	334
PkrI	GCNGC	4 cut(s)	38, 41, 257, 1093
Ple19I	CGATCG	1 cut(s)	33
PleI	GAGTC	2 cut(s)	310, 1210
PluTI	GGCGCC	1 cut(s)	509
PpsI	GAGTC	2 cut(s)	310, 1210
Ppu21I	YACGTR	2 cut(s)	225, 247
Psp124BI	GAGCTC	3 cut(s)	167, 297, 812
Psp6I	CCWGG	1 cut(s)	602
PspGI	CCWGG	1 cut(s)	602
PspN4I	GGNNCC	4 cut(s)	43, 507, 592, 1021
PspPI	GGNCC	3 cut(s)	23, 591, 1476
PspXI	VCTCGAGB	1 cut(s)	296
PvuI	CGATCG	1 cut(s)	33
RsaI	GTAC	7 cut(s)	185, 227, 245, 249, 568, 949, 1416
RsaNI	GTAC	7 cut(s)	184, 226, 244, 248, 567, 948, 1415
RseI	CAYNNNNRTG	3 cut(s)	360, 401, 494
SacI	GAGCTC	3 cut(s)	167, 297, 812
SacII	CCGCGG	1 cut(s)	40
SapI	GCTCTTC	1 cut(s)	522
SaqAI	TTAA	4 cut(s)	384, 1371, 1385, 1472
SatI	GCNGC	4 cut(s)	37, 40, 256, 1092
Sau3AI	GATC	8 cut(s)	30, 309, 423, 436, 832, 927, 939, 1432
Sau96I	GGNCC	3 cut(s)	23, 591, 1476
ScaI	AGTACT	1 cut(s)	185
SchI	GAGTC	2 cut(s)	311, 1211
ScrFI	CCNGG	2 cut(s)	252, 604
SduI	GDGCHC	5 cut(s)	167, 297, 559, 812, 1004
SfaNI	GCATC	3 cut(s)	259, 476, 775
SfiI	GGCCNNNNNGGCC	1 cut(s)	590
SfoI	GGCGCC	1 cut(s)	507
Sfr274I	CTCGAG	1 cut(s)	296
Sfr303I	CCGCGG	1 cut(s)	40
SfuI	TTCGAA	1 cut(s)	277
SgrAI	CRCCGGYG	1 cut(s)	493
SgrBI	CCGCGG	1 cut(s)	40
SinI	GGWCC	1 cut(s)	23
SlaI	CTCGAG	1 cut(s)	296
SmiMI	CAYNNNNRTG	3 cut(s)	360, 401, 494
SmlI	CTYRAG	4 cut(s)	296, 706, 761, 1370
SmoI	CTYRAG	4 cut(s)	296, 706, 761, 1370
SnaBI	TACGTA	2 cut(s)	225, 247
SseBI	AGGCCT	1 cut(s)	840
SsiI	CCGC	8 cut(s)	37, 39, 48, 95, 255, 522, 917, 1091
SspDI	GGCGCC	1 cut(s)	505
SspMI	CTAG	2 cut(s)	18, 62
SstI	GAGCTC	3 cut(s)	167, 297, 812
StuI	AGGCCT	1 cut(s)	840
StyD4I	CCNGG	2 cut(s)	250, 602
TaaI	ACNGT	5 cut(s)	55, 1006, 1099, 1142, 1211
TaiI	ACGT	3 cut(s)	227, 249, 476
TaqI	TCGA	3 cut(s)	277, 297, 1492
TatI	WGTACW	2 cut(s)	183, 566
TauI	GCSGC	4 cut(s)	39, 42, 258, 1094
TfiI	GAWTC	7 cut(s)	274, 648, 848, 880, 1115, 1184, 1262
Tru1I	TTAA	4 cut(s)	384, 1371, 1385, 1472
Tru9I	TTAA	4 cut(s)	384, 1371, 1385, 1472
TscAI	CASTG	3 cut(s)	149, 211, 1009
TseFI	GTSAC	2 cut(s)	377, 656
Tsp45I	GTSAC	2 cut(s)	377, 656
TspDTI	ATGAA	7 cut(s)	226, 344, 477, 490, 840, 1176, 1233
TspRI	CASTG	3 cut(s)	149, 211, 1009
Van91I	CCANNNNNTGG	1 cut(s)	334
Vha464I	CTTAAG	1 cut(s)	1370
VneI	GTGCAC	1 cut(s)	1000
VpaK11BI	GGWCC	1 cut(s)	23
XapI	RAATTY	1 cut(s)	983
XceI	RCATGY	1 cut(s)	729
XcmI	CCANNNNNNNNNTGG	1 cut(s)	738
XhoI	CTCGAG	1 cut(s)	296
XmiI	GTMKAC	2 cut(s)	797, 970
XspI	CTAG	2 cut(s)	18, 62
ZrmI	AGTACT	1 cut(s)	185
Zsp2I	ATGCAT	1 cut(s)	1459

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.