Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0017265

Physical Location & Seq

Genomic Coordinates Forward (+)

31245585 .. 31247168

Gene Structure 1584 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1213700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGTCACGTATAAATAATGAGCATTTAGCCATTCAGGCATACATTTCAAAAATCGCCACCAAACATAAAACTCAAAACTCTCACATTTTCTCTTCTGCCATGGAGCACTGGACCCGAGGACCGACCGTCGGCCGCGGCTCCACTGCCACAGTCTCCCTCGCCACCAGCACCATCTCCGGCGAACGATTTGCGGTGAAGTCTACCGAGCTCTCCAGCTCGTCTTTTTTGCAGAAGGAGCAATGTTTCTTGTCCAAGCTAAGTTGCCCCCATATAGTTAAGTACTTGGGCTGCAATGTTAGCATTGAAAACAACGAGCATATGTACAATCTCTGCATGGAGTACGTATCCGGCGGCACGCTTTTCGATGCGATTCGGAGGCGGGGAGGGCCGCTAGAGGAGGCCATGATCCAACTGTACACACATCAAATTGTGCAAGGATTGGAGTATTTGCATGAGAATAAGTTGGTACATTGTGACATCAAGAGCCAAAACATTTTGGCGACCGAAGAGAGCGCCAAAATTGCCGATTTGGGTTGTGCTAAATTGGTACAAGGAGTTGCAGAGGACGAATACTCTGTCGAGGCAAGTGCTACTATTTCGGGTACGCCGGTGTTTATGGCGCCAGAGGTTGCTCGCGGCGAGGAGCAGGGGTTTGGGGCTGACATATGGGCTCTAGGGTGCACAATTATAGAAATGGCAACCGGTGGCGGGCCTTGGCCGGAGATAAATGATCCGGTTTCAGCTATCTACCGGATTGGGTTCTCCGGTGAGGTACCGGAGATTCCAAGCCGACTTTCGAGCAAGGGTAAGGATTTCTTGAGCAAGTGTTTGATGAAAGATCCAAGAGAGAGGTGGACAGCTAAGCAGCTTCTTGAGCATCCATTTCTGGAAGAGCATTTGGGGTCCAAGTTCAATACAGAGCAAGTAATGGGGTCTCCAATTAGTGTGTTGGATCAAGGCCTTTGGGATTCATTTGAAGGATCTGAAAGTCCAACGGGATTGCAATCTGTTGAGGTTGAAGGTGTTTGTTTGAATTCTCCAACAGAAAGGATCAAGAAACTGATTGGATTTTCGACATCTTCATATGCACCCAATTGGGAATTTGACGAAAGTTGGTCAACTGTTAGAAGCAATGGCGACGAAGAACTCGAAAACTTGGATGTTGCAAACAACAATGTCGTCTCAGACGATGAAGTTTTTGCTGCAACTTCTTCAACTGTGGCTTCAATTGTTCTTGAAGAGACTGAGAGTTTAAACTCCGATGAGGATTTAGATTTTGAATTTATGATTAGTAATAGTGTGATAGAAGATTATTTTGTATCATACCATCTTGAAATTGAATCAGATAATCAGAATTATGATTTTATTCACTCCCATTGTTATCATTCGTGTCTTAGTTTTGCCTGCCAAAGTAACATTAGTGGAGTTTCTTTTCTATTTTGTTTTAGTTTTAGTAGTTCCAACTTCCAAGGGCGACAAGCCTATAGCATTGACAAATCACCGACTAGCTACCCGTTTAGCGATCCTGCTGCTCCCAATACTAGAAGAGGTCTCAAACTTGAATTGTCAAAGTTTCATAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009653 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0099120 GO:0099568 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

528

Amino Acids

58.33

Weight (kDa)

4.82

Isoelectric Point (pI)

51.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	39 - 296	2e-57	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	42 - 292	3.7e-34	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0017265)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g23120
malus_domestica	MD00G1159200.v1.1 MD11G1213700.v1.1
prunus_persica	Prupe.4G217600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0040771
rosa_laevigata	RLG00000034000
rosa_multiflora	Rmu_ssc0000274.1_g000002
rosa_rugosa	Rorug05G0187100
rosa_samantha	Rh5AG272900 Rh5BG278000 Rh5CG310000 Rh5DG285900
rosa_wichuraiana	Rw5G025660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	772
AccB1I	GGYRCC	2 cut(s)	619, 772
AccI	GTMKAC	1 cut(s)	200
AccII	CGCG	2 cut(s)	135, 636
AciI	CCGC	8 cut(s)	133, 135, 191, 351, 379, 389, 636, 708
AclWI	GGATC	7 cut(s)	400, 725, 833, 960, 988, 1058, 1517
AcoI	YGGCCR	2 cut(s)	130, 716
AcsI	RAATTY	3 cut(s)	1033, 1100, 1280
AcyI	GRCGYC	1 cut(s)	620
AfaI	GTAC	8 cut(s)	281, 323, 341, 416, 468, 549, 604, 774
AfiI	CCNNNNNNNGG	2 cut(s)	128, 708
AgeI	ACCGGT	1 cut(s)	701
AhdI	GACNNNNNGTC	1 cut(s)	125
AjuI	GAANNNNNNNTTGG	2 cut(s)	778, 810
AluBI	AGCT	7 cut(s)	208, 216, 256, 743, 860, 868, 1509
AluI	AGCT	7 cut(s)	208, 216, 256, 743, 860, 868, 1509
Alw21I	GWGCWC	3 cut(s)	108, 210, 683
Alw26I	GTCTC	5 cut(s)	157, 939, 1186, 1235, 1556
Alw44I	GTGCAC	1 cut(s)	679
AlwI	GGATC	7 cut(s)	400, 725, 833, 960, 988, 1058, 1517
Ama87I	CYCGRG	1 cut(s)	114
AoxI	GGCC	6 cut(s)	130, 386, 399, 710, 716, 958
ApaLI	GTGCAC	1 cut(s)	679
ApeKI	GCWGC	4 cut(s)	288, 865, 1202, 1529
ApoI	RAATTY	3 cut(s)	1033, 1100, 1280
ArsI	GACNNNNNNTTYG	2 cut(s)	561, 593
AsiGI	ACCGGT	1 cut(s)	701
Asp718I	GGTACC	1 cut(s)	772
AspLEI	GCGC	2 cut(s)	515, 622
AspS9I	GGNCC	5 cut(s)	111, 119, 386, 710, 903
AsuHPI	GGTGA	3 cut(s)	205, 779, 1491
AvaI	CYCGRG	1 cut(s)	114
AvaII	GGWCC	3 cut(s)	111, 119, 903
BaeGI	GKGCMC	1 cut(s)	683
BaeI	ACNNNNGTAYC	2 cut(s)	594, 627
BanI	GGYRCC	2 cut(s)	619, 772
BanII	GRGCYC	2 cut(s)	210, 673
Bbv12I	GWGCWC	3 cut(s)	108, 210, 683
BbvI	GCAGC	4 cut(s)	275, 877, 1189, 1516
BccI	CCATC	2 cut(s)	179, 1335
BcgI	CGANNNNNNTGC	8 cut(s)	170, 204, 343, 377, 569, 603, 1511, 1545
BciVI	GTATCC	1 cut(s)	355
BcoDI	GTCTC	5 cut(s)	157, 939, 1186, 1235, 1556
BfaI	CTAG	4 cut(s)	392, 674, 1506, 1542
BfmI	CTRYAG	1 cut(s)	1483
BfoI	RGCGCY	2 cut(s)	516, 623
BfuI	GTATCC	1 cut(s)	355
BglI	GCCNNNNNGGC	1 cut(s)	35
BisI	GCNGC	9 cut(s)	133, 136, 289, 352, 389, 637, 866, 1203, 1530
BlpI	GCTNAGC	1 cut(s)	861
BlsI	GCNGC	9 cut(s)	134, 137, 290, 353, 390, 638, 867, 1204, 1531
BmcAI	AGTACT	1 cut(s)	281
Bme18I	GGWCC	3 cut(s)	111, 119, 903
BmeRI	GACNNNNNGTC	1 cut(s)	125
BmeT110I	CYCGRG	1 cut(s)	114
BmgT120I	GGNCC	5 cut(s)	111, 119, 386, 710, 903
BmiI	GGNNCC	5 cut(s)	113, 139, 621, 774, 904
BmsI	GCATC	2 cut(s)	355, 886
BpmI	CTGGAG	1 cut(s)	196
Bpu1102I	GCTNAGC	1 cut(s)	861
BpuEI	CTTGAG	2 cut(s)	838, 893
BsaAI	YACGTR	2 cut(s)	8, 343
BsaBI	GATNNNNATC	1 cut(s)	1003
BsaHI	GRCGYC	1 cut(s)	620
BsaI	GGTCTC	2 cut(s)	939, 1556
BsaJI	CCNNGG	5 cut(s)	99, 115, 133, 713, 1468
BsaWI	WCCGGW	5 cut(s)	701, 733, 750, 764, 775
BsaXI	ACNNNNNCTCC	2 cut(s)	635, 665
Bsc4I	CCNNNNNNNGG	2 cut(s)	128, 708
Bse118I	RCCGGY	2 cut(s)	607, 701
Bse1I	ACTGG	1 cut(s)	113
Bse3DI	GCAATG	3 cut(s)	245, 298, 1138
Bse8I	GATNNNNATC	1 cut(s)	1003
BseDI	CCNNGG	5 cut(s)	99, 115, 133, 713, 1468
BseGI	GGATG	2 cut(s)	877, 1165
BseJI	GATNNNNATC	1 cut(s)	1003
BseLI	CCNNNNNNNGG	2 cut(s)	128, 708
BseMI	GCAATG	3 cut(s)	245, 298, 1138
BseMII	CTCAG	2 cut(s)	1197, 1236
BseNI	ACTGG	1 cut(s)	113
BseRI	GAGGAG	2 cut(s)	410, 656
BseSI	GKGCMC	1 cut(s)	683
BseX3I	CGGCCG	1 cut(s)	130
BseXI	GCAGC	4 cut(s)	275, 877, 1189, 1516
Bsh1236I	CGCG	2 cut(s)	135, 636
Bsh1285I	CGRYCG	3 cut(s)	126, 133, 504
BshFI	GGCC	6 cut(s)	132, 388, 401, 712, 718, 960
BshNI	GGYRCC	2 cut(s)	619, 772
BshTI	ACCGGT	1 cut(s)	701
BsiEI	CGRYCG	3 cut(s)	126, 133, 504
BsiHKAI	GWGCWC	3 cut(s)	108, 210, 683
BsiHKCI	CYCGRG	1 cut(s)	114
BsiSI	CCGG	9 cut(s)	177, 348, 608, 702, 719, 734, 751, 765, 776
BslI	CCNNNNNNNGG	2 cut(s)	128, 708
BsmAI	GTCTC	5 cut(s)	157, 939, 1186, 1235, 1556
BsmBI	CGTCTC	1 cut(s)	1186
BsnI	GGCC	6 cut(s)	132, 388, 401, 712, 718, 960
Bso31I	GGTCTC	2 cut(s)	939, 1556
BsoBI	CYCGRG	1 cut(s)	114
Bsp1286I	GDGCHC	4 cut(s)	108, 210, 673, 683
Bsp1407I	TGTACA	2 cut(s)	321, 414
Bsp143I	GATC	7 cut(s)	405, 730, 838, 952, 980, 1050, 1522
Bsp1720I	GCTNAGC	1 cut(s)	861
Bsp19I	CCATGG	1 cut(s)	99
BspACI	CCGC	8 cut(s)	133, 135, 191, 351, 379, 389, 636, 708
BspANI	GGCC	6 cut(s)	132, 388, 401, 712, 718, 960
BspCNI	CTCAG	2 cut(s)	1196, 1237
BspFNI	CGCG	2 cut(s)	135, 636
BspLI	GGNNCC	5 cut(s)	113, 139, 621, 774, 904
BspPI	GGATC	7 cut(s)	400, 725, 833, 960, 988, 1058, 1517
BspQI	GCTCTTC	1 cut(s)	885
BspT107I	GGYRCC	2 cut(s)	619, 772
BspTNI	GGTCTC	2 cut(s)	939, 1556
BsrDI	GCAATG	3 cut(s)	245, 298, 1138
BsrFI	RCCGGY	2 cut(s)	607, 701
BsrGI	TGTACA	2 cut(s)	321, 414
BsrI	ACTGG	1 cut(s)	113
BssAI	RCCGGY	2 cut(s)	607, 701
BssECI	CCNNGG	5 cut(s)	99, 115, 133, 713, 1468
BssMI	GATC	7 cut(s)	405, 730, 838, 952, 980, 1050, 1522
BssNI	GRCGYC	1 cut(s)	620
BssT1I	CCWWGG	3 cut(s)	99, 713, 1468
Bst4CI	ACNGT	5 cut(s)	127, 151, 414, 1123, 1219
Bst6I	CTCTTC	5 cut(s)	97, 501, 885, 1233, 1540
BstACI	GRCGYC	1 cut(s)	620
BstAUI	TGTACA	2 cut(s)	321, 414
BstBAI	YACGTR	2 cut(s)	8, 343
BstC8I	GCNNGC	4 cut(s)	356, 634, 710, 1405
BstDEI	CTNAG	5 cut(s)	257, 861, 1183, 1245, 1394
BstDSI	CCRYGG	2 cut(s)	99, 133
BstF5I	GGATG	2 cut(s)	877, 1165
BstFNI	CGCG	2 cut(s)	135, 636
BstH2I	RGCGCY	2 cut(s)	516, 623
BstHHI	GCGC	2 cut(s)	515, 622
BstKTI	GATC	7 cut(s)	408, 733, 841, 955, 983, 1053, 1525
BstMAI	GTCTC	5 cut(s)	157, 939, 1186, 1235, 1556
BstMBI	GATC	7 cut(s)	405, 730, 838, 952, 980, 1050, 1522
BstMCI	CGRYCG	3 cut(s)	126, 133, 504
BstMWI	GCNNNNNNNGC	6 cut(s)	35, 235, 297, 385, 521, 874
BstSFI	CTRYAG	1 cut(s)	1483
BstSLI	GKGCMC	1 cut(s)	683
BstSNI	TACGTA	1 cut(s)	343
BstUI	CGCG	2 cut(s)	135, 636
BstV1I	GCAGC	4 cut(s)	275, 877, 1189, 1516
BstX2I	RGATCY	2 cut(s)	838, 980
BstYI	RGATCY	2 cut(s)	838, 980
BstZI	CGGCCG	1 cut(s)	130
BsuI	GTATCC	1 cut(s)	355
BsuRI	GGCC	6 cut(s)	132, 388, 401, 712, 718, 960
BtgI	CCRYGG	2 cut(s)	99, 133
BtsCI	GGATG	2 cut(s)	877, 1165
BtsI	GCAGTG	1 cut(s)	141
BtsIMutI	CAGTG	2 cut(s)	106, 141
Cac8I	GCNNGC	4 cut(s)	356, 634, 710, 1405
CfoI	GCGC	2 cut(s)	515, 622
Cfr10I	RCCGGY	2 cut(s)	607, 701
Cfr13I	GGNCC	5 cut(s)	111, 119, 386, 710, 903
Cfr42I	CCGCGG	1 cut(s)	136
Csp6I	GTAC	8 cut(s)	280, 322, 340, 415, 467, 548, 603, 773
CspAI	ACCGGT	1 cut(s)	701
CviAII	CATG	4 cut(s)	100, 334, 403, 452
CviQI	GTAC	8 cut(s)	280, 322, 340, 415, 467, 548, 603, 773
DdeI	CTNAG	5 cut(s)	257, 861, 1183, 1245, 1394
DinI	GGCGCC	1 cut(s)	621
DpnI	GATC	7 cut(s)	407, 732, 840, 954, 982, 1052, 1524
DpnII	GATC	7 cut(s)	405, 730, 838, 952, 980, 1050, 1522
DraI	TTTAAA	1 cut(s)	1254
DriI	GACNNNNNGTC	1 cut(s)	125
EaeI	YGGCCR	2 cut(s)	130, 716
EagI	CGGCCG	1 cut(s)	130
Eam1104I	CTCTTC	5 cut(s)	97, 501, 885, 1233, 1540
Eam1105I	GACNNNNNGTC	1 cut(s)	125
EarI	CTCTTC	5 cut(s)	97, 501, 885, 1233, 1540
Ecl136II	GAGCTC	1 cut(s)	208
EclXI	CGGCCG	1 cut(s)	130
Eco105I	TACGTA	1 cut(s)	343
Eco130I	CCWWGG	3 cut(s)	99, 713, 1468
Eco147I	AGGCCT	1 cut(s)	960
Eco24I	GRGCYC	2 cut(s)	210, 673
Eco31I	GGTCTC	2 cut(s)	939, 1556
Eco47I	GGWCC	3 cut(s)	111, 119, 903
Eco52I	CGGCCG	1 cut(s)	130
Eco53kI	GAGCTC	1 cut(s)	208
Eco88I	CYCGRG	1 cut(s)	114
EcoICRI	GAGCTC	1 cut(s)	208
EcoRI	GAATTC	1 cut(s)	1033
EcoT14I	CCWWGG	3 cut(s)	99, 713, 1468
EcoT38I	GRGCYC	2 cut(s)	210, 673
EgeI	GGCGCC	1 cut(s)	621
EheI	GGCGCC	1 cut(s)	621
ErhI	CCWWGG	3 cut(s)	99, 713, 1468
Esp3I	CGTCTC	1 cut(s)	1186
FaeI	CATG	4 cut(s)	103, 337, 406, 455
FalI	AAGNNNNNCTT	2 cut(s)	800, 832
FatI	CATG	4 cut(s)	99, 333, 402, 451
FauI	CCCGC	2 cut(s)	372, 701
FauNDI	CATATG	3 cut(s)	318, 665, 1084
FblI	GTMKAC	1 cut(s)	200
Fnu4HI	GCNGC	9 cut(s)	133, 136, 289, 352, 389, 637, 866, 1203, 1530
FokI	GGATG	2 cut(s)	864, 1172
FriOI	GRGCYC	2 cut(s)	210, 673
Fsp4HI	GCNGC	9 cut(s)	133, 136, 289, 352, 389, 637, 866, 1203, 1530
FspBI	CTAG	4 cut(s)	392, 674, 1506, 1542
GlaI	GCGC	2 cut(s)	514, 621
GluI	GCNGC	9 cut(s)	133, 136, 289, 352, 389, 637, 866, 1203, 1530
GsuI	CTGGAG	1 cut(s)	196
HaeII	RGCGCY	2 cut(s)	516, 623
HaeIII	GGCC	6 cut(s)	132, 388, 401, 712, 718, 960
HapII	CCGG	9 cut(s)	177, 348, 608, 702, 719, 734, 751, 765, 776
HhaI	GCGC	2 cut(s)	515, 622
Hin1I	GRCGYC	1 cut(s)	620
Hin1II	CATG	4 cut(s)	103, 337, 406, 455
Hin6I	GCGC	2 cut(s)	513, 620
HinP1I	GCGC	2 cut(s)	513, 620
HincII	GTYRAC	1 cut(s)	1119
HindII	GTYRAC	1 cut(s)	1119
HinfI	GANTC	4 cut(s)	370, 781, 968, 1340
HpaII	CCGG	9 cut(s)	177, 348, 608, 702, 719, 734, 751, 765, 776
HphI	GGTGA	3 cut(s)	205, 779, 1491
Hpy166II	GTNNAC	5 cut(s)	201, 417, 681, 855, 1119
Hpy188I	TCNGA	6 cut(s)	375, 985, 1186, 1261, 1345, 1353
Hpy188III	TCNNGA	7 cut(s)	481, 817, 872, 887, 1054, 1235, 1331
Hpy8I	GTNNAC	5 cut(s)	201, 417, 681, 855, 1119
Hpy99I	CGWCG	2 cut(s)	131, 1142
HpyAV	CCTTC	3 cut(s)	226, 971, 1013
HpyCH4III	ACNGT	5 cut(s)	127, 151, 414, 1123, 1219
HpyCH4IV	ACGT	2 cut(s)	7, 342
HpyF10VI	GCNNNNNNNGC	6 cut(s)	35, 235, 297, 385, 521, 874
HpyF3I	CTNAG	5 cut(s)	257, 861, 1183, 1245, 1394
HpySE526I	ACGT	2 cut(s)	7, 342
Hsp92I	GRCGYC	1 cut(s)	620
Hsp92II	CATG	4 cut(s)	103, 337, 406, 455
HspAI	GCGC	2 cut(s)	513, 620
KasI	GGCGCC	1 cut(s)	619
KpnI	GGTACC	1 cut(s)	776
KspI	CCGCGG	1 cut(s)	136
Kzo9I	GATC	7 cut(s)	405, 730, 838, 952, 980, 1050, 1522
LguI	GCTCTTC	1 cut(s)	885
LmnI	GCTCC	5 cut(s)	103, 143, 235, 643, 1537
Lsp1109I	GCAGC	4 cut(s)	275, 877, 1189, 1516
LweI	GCATC	2 cut(s)	355, 886
MaeI	CTAG	4 cut(s)	392, 674, 1506, 1542
MaeII	ACGT	2 cut(s)	7, 342
MaeIII	GTNAC	3 cut(s)	3, 473, 1412
MalI	GATC	7 cut(s)	407, 732, 840, 954, 982, 1052, 1524
MboI	GATC	7 cut(s)	405, 730, 838, 952, 980, 1050, 1522
MboII	GAAGA	9 cut(s)	84, 518, 902, 1071, 1154, 1203, 1250, 1319, 1557
MfeI	CAATTG	2 cut(s)	1093, 1227
MflI	RGATCY	2 cut(s)	838, 980
MhlI	GDGCHC	4 cut(s)	108, 210, 673, 683
Mly113I	GGCGCC	1 cut(s)	620
MmeI	TCCRAC	5 cut(s)	433, 930, 1016, 1064, 1485
MseI	TTAA	2 cut(s)	276, 1253
MspA1I	CMGCKG	1 cut(s)	135
MspI	CCGG	9 cut(s)	177, 348, 608, 702, 719, 734, 751, 765, 776
MssI	GTTTAAAC	1 cut(s)	1254
MunI	CAATTG	2 cut(s)	1093, 1227
MvnI	CGCG	2 cut(s)	135, 636
MwoI	GCNNNNNNNGC	6 cut(s)	35, 235, 297, 385, 521, 874
NarI	GGCGCC	1 cut(s)	620
NcoI	CCATGG	1 cut(s)	99
NdeI	CATATG	3 cut(s)	318, 665, 1084
NdeII	GATC	7 cut(s)	405, 730, 838, 952, 980, 1050, 1522
NlaIII	CATG	4 cut(s)	103, 337, 406, 455
NlaIV	GGNNCC	5 cut(s)	113, 139, 621, 774, 904
NmuCI	GTSAC	2 cut(s)	3, 473
PceI	AGGCCT	1 cut(s)	960
PciSI	GCTCTTC	1 cut(s)	885
PcsI	WCGNNNNNNNCGW	2 cut(s)	1146, 1185
PfeI	GAWTC	4 cut(s)	370, 781, 968, 1340
PinAI	ACCGGT	1 cut(s)	701
PkrI	GCNGC	9 cut(s)	134, 137, 290, 353, 390, 638, 867, 1204, 1531
PluTI	GGCGCC	1 cut(s)	623
PmeI	GTTTAAAC	1 cut(s)	1254
Ppu21I	YACGTR	2 cut(s)	8, 343
Psp124BI	GAGCTC	1 cut(s)	210
PspN4I	GGNNCC	5 cut(s)	113, 139, 621, 774, 904
PspPI	GGNCC	5 cut(s)	111, 119, 386, 710, 903
PsuI	RGATCY	2 cut(s)	838, 980
RsaI	GTAC	8 cut(s)	281, 323, 341, 416, 468, 549, 604, 774
RsaNI	GTAC	8 cut(s)	280, 322, 340, 415, 467, 548, 603, 773
SacI	GAGCTC	1 cut(s)	210
SacII	CCGCGG	1 cut(s)	136
SapI	GCTCTTC	1 cut(s)	885
SaqAI	TTAA	2 cut(s)	276, 1253
SatI	GCNGC	9 cut(s)	133, 136, 289, 352, 389, 637, 866, 1203, 1530
Sau3AI	GATC	7 cut(s)	405, 730, 838, 952, 980, 1050, 1522
Sau96I	GGNCC	5 cut(s)	111, 119, 386, 710, 903
ScaI	AGTACT	1 cut(s)	281
SduI	GDGCHC	4 cut(s)	108, 210, 673, 683
SfaNI	GCATC	2 cut(s)	355, 886
SfcI	CTRYAG	1 cut(s)	1483
SfoI	GGCGCC	1 cut(s)	621
Sfr303I	CCGCGG	1 cut(s)	136
SgrAI	CRCCGGYG	1 cut(s)	607
SgrBI	CCGCGG	1 cut(s)	136
SinI	GGWCC	3 cut(s)	111, 119, 903
SmlI	CTYRAG	2 cut(s)	817, 872
SmoI	CTYRAG	2 cut(s)	817, 872
SnaBI	TACGTA	1 cut(s)	343
SseBI	AGGCCT	1 cut(s)	960
SsiI	CCGC	8 cut(s)	133, 135, 191, 351, 379, 389, 636, 708
SspDI	GGCGCC	1 cut(s)	619
SspMI	CTAG	4 cut(s)	392, 674, 1506, 1542
SstI	GAGCTC	1 cut(s)	210
StuI	AGGCCT	1 cut(s)	960
StyI	CCWWGG	3 cut(s)	99, 713, 1468
TaaI	ACNGT	5 cut(s)	127, 151, 414, 1123, 1219
TaiI	ACGT	2 cut(s)	10, 345
TaqI	TCGA	5 cut(s)	363, 579, 797, 1073, 1149
TaqII	GACCGA	2 cut(s)	136, 518
TatI	WGTACW	3 cut(s)	279, 321, 414
TauI	GCSGC	5 cut(s)	135, 138, 354, 391, 639
TfiI	GAWTC	4 cut(s)	370, 781, 968, 1340
Tru1I	TTAA	2 cut(s)	276, 1253
Tru9I	TTAA	2 cut(s)	276, 1253
TscAI	CASTG	2 cut(s)	113, 148
TseFI	GTSAC	2 cut(s)	3, 473
TseI	GCWGC	4 cut(s)	288, 865, 1202, 1529
Tsp45I	GTSAC	2 cut(s)	3, 473
TspDTI	ATGAA	5 cut(s)	848, 960, 1071, 1206, 1565
TspRI	CASTG	2 cut(s)	113, 148
VneI	GTGCAC	1 cut(s)	679
VpaK11BI	GGWCC	3 cut(s)	111, 119, 903
XapI	RAATTY	3 cut(s)	1033, 1100, 1280
XcmI	CCANNNNNNNNNTGG	1 cut(s)	849
XmiI	GTMKAC	1 cut(s)	200
XspI	CTAG	4 cut(s)	392, 674, 1506, 1542
ZrmI	AGTACT	1 cut(s)	281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.