MD00G1206700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015228
Physical Location & Seq
Reverse (-)
50320759 .. 50321100
342 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1206700.v1.1.491

Sequence Viewer

Length: 342 bp
ATGGATGTGATGATCAGAAAGTCGTACGGCGGCTACTCGAAGTTGGACAAGGAAGATCCTGAAGATGTAAATCATCGACGGGCGCAGTTCCTGATCTACAAAGTGTTGCTGCAAGCAGAAATGCGGCGAAGGCCTTCGAATTTGAGGATTCGGATACGTAAACTGAAGGTGAAGATTGGGAGGAGATTAAAGAAGTTGAGGAAGAGCATGCTTCTAGGTATATCTGCCGCCAGGGTTTGTGTTTATAAGCGAGTTTTTAATCAACTAAAAACTTGCATGACCTTGTTTGGCCGCGGAGATCAGGGAACTGTCAATGCAACTCTTCCAGTTTTGCTTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

13.18

Weight (kDa)

11.02

Isoelectric Point (pI)

39.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11655
fragaria_vesca FvH4_3g02270
malus_domestica MD00G1206700.v1.1 MD05G1344200.v1.1 MD10G1320200.v1.1
prunus_persica Prupe.4G022200_v2.0.a1
pyrus_communis pycom05g31360
rosa_chinensis RchiOBHm_Chr5g0003391
rosa_laevigata RLG00000031135
rosa_multiflora Rmu_co8199484.1_g000001 Rmu_sc0010917.1_g000001
rosa_roxburghii Rroxscaffold_1G00072630
rosa_rugosa Rorug04G0403000
rosa_samantha Rh5AG029600 Rh5CG031800
rosa_wichuraiana Rw5G002770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 246
AccII CGCG 1 cut(s) 294
AciI CCGC 5 cut(s) 30, 124, 228, 292, 294
AclWI GGATC 1 cut(s) 50
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 1 cut(s) 139
AcuI CTGAAG 2 cut(s) 81, 185
AfaI GTAC 1 cut(s) 26
AjnI CCWGG 1 cut(s) 230
AlwI GGATC 1 cut(s) 50
AlwNI CAGNNNCTG 1 cut(s) 91
AoxI GGCC 2 cut(s) 131, 289
ApeKI GCWGC 1 cut(s) 109
ApoI RAATTY 1 cut(s) 139
Asp700I GAANNNNTTC 1 cut(s) 133
AspLEI GCGC 1 cut(s) 85
AsuHPI GGTGA 1 cut(s) 181
AsuII TTCGAA 1 cut(s) 137
BbvI GCAGC 1 cut(s) 96
BceAI ACGGC 1 cut(s) 43
BciT130I CCWGG 1 cut(s) 232
BciVI GTATCC 1 cut(s) 147
BclI TGATCA 1 cut(s) 12
BfaI CTAG 1 cut(s) 215
BfuI GTATCC 1 cut(s) 147
BisI GCNGC 5 cut(s) 31, 110, 125, 228, 292
BlsI GCNGC 5 cut(s) 32, 111, 126, 229, 293
Bme1390I CCNGG 1 cut(s) 232
BmrFI CCNGG 1 cut(s) 232
Bpu14I TTCGAA 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 158
BsaBI GATNNNNATC 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 231, 292
Bse1I ACTGG 1 cut(s) 326
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 2 cut(s) 231, 292
BseGI GGATG 1 cut(s) 10
BseJI GATNNNNATC 1 cut(s) 69
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 96
Bsh1236I CGCG 1 cut(s) 294
BshFI GGCC 2 cut(s) 133, 291
BsiWI CGTACG 1 cut(s) 24
BsnI GGCC 2 cut(s) 133, 291
Bsp119I TTCGAA 1 cut(s) 137
Bsp143I GATC 4 cut(s) 12, 55, 93, 298
BspACI CCGC 5 cut(s) 30, 124, 228, 292, 294
BspANI GGCC 2 cut(s) 133, 291
BspFNI CGCG 1 cut(s) 294
BspPI GGATC 1 cut(s) 50
BspQI GCTCTTC 1 cut(s) 197
BspT104I TTCGAA 1 cut(s) 137
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 2 cut(s) 231, 292
BssMI GATC 4 cut(s) 12, 55, 93, 298
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 2 cut(s) 197, 327
BstBAI YACGTR 1 cut(s) 158
BstBI TTCGAA 1 cut(s) 137
BstC8I GCNNGC 2 cut(s) 114, 209
BstDSI CCRYGG 1 cut(s) 292
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 294
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 4 cut(s) 15, 58, 96, 301
BstMBI GATC 4 cut(s) 12, 55, 93, 298
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 230
BstSNI TACGTA 1 cut(s) 158
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BsuI GTATCC 1 cut(s) 147
BsuRI GGCC 2 cut(s) 133, 291
BtgI CCRYGG 1 cut(s) 292
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 2 cut(s) 114, 209
CaiI CAGNNNCTG 1 cut(s) 91
CfoI GCGC 1 cut(s) 85
Cfr42I CCGCGG 1 cut(s) 295
Csp6I GTAC 1 cut(s) 25
CviAII CATG 2 cut(s) 208, 277
CviJI RGCY 3 cut(s) 33, 133, 291
CviKI_1 RGCY 3 cut(s) 33, 133, 291
CviQI GTAC 1 cut(s) 25
DpnI GATC 4 cut(s) 14, 57, 95, 300
DpnII GATC 4 cut(s) 12, 55, 93, 298
EaeI YGGCCR 1 cut(s) 289
Eam1104I CTCTTC 2 cut(s) 197, 327
EarI CTCTTC 2 cut(s) 197, 327
Eco105I TACGTA 1 cut(s) 158
Eco147I AGGCCT 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 81, 185
EcoRII CCWGG 1 cut(s) 230
FaeI CATG 2 cut(s) 211, 280
FaiI YATR 4 cut(s) 209, 221, 246, 278
FatI CATG 2 cut(s) 207, 276
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 5 cut(s) 31, 110, 125, 228, 292
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 5 cut(s) 31, 110, 125, 228, 292
FspBI CTAG 1 cut(s) 215
GlaI GCGC 1 cut(s) 84
GluI GCNGC 5 cut(s) 31, 110, 125, 228, 292
HaeIII GGCC 2 cut(s) 133, 291
HhaI GCGC 1 cut(s) 85
Hin1II CATG 2 cut(s) 211, 280
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HinfI GANTC 1 cut(s) 148
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 161
Hpy188I TCNGA 2 cut(s) 17, 153
Hpy188III TCNNGA 2 cut(s) 59, 91
Hpy8I GTNNAC 1 cut(s) 161
Hpy99I CGWCG 1 cut(s) 81
HpyAV CCTTC 3 cut(s) 123, 144, 160
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 1 cut(s) 157
HpyCH4V TGCA 3 cut(s) 112, 276, 317
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 2 cut(s) 211, 280
HspAI GCGC 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 12
KspI CCGCGG 1 cut(s) 295
Kzo9I GATC 4 cut(s) 12, 55, 93, 298
LguI GCTCTTC 1 cut(s) 197
LpnPI CCDG 5 cut(s) 72, 104, 217, 244, 287
Lsp1109I GCAGC 1 cut(s) 96
MaeI CTAG 1 cut(s) 215
MaeII ACGT 1 cut(s) 157
MalI GATC 4 cut(s) 14, 57, 95, 300
MboI GATC 4 cut(s) 12, 55, 93, 298
MboII GAAGA 5 cut(s) 65, 74, 184, 214, 314
MflI RGATCY 1 cut(s) 55
MluCI AATT 1 cut(s) 139
MmeI TCCRAC 1 cut(s) 24
MnlI CCTC 3 cut(s) 138, 174, 192
MroXI GAANNNNTTC 1 cut(s) 133
MseI TTAA 2 cut(s) 188, 258
MspA1I CMGCKG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 232
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 1 cut(s) 130
NdeII GATC 4 cut(s) 12, 55, 93, 298
NlaIII CATG 2 cut(s) 211, 280
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 1 cut(s) 137
PaeI GCATGC 1 cut(s) 211
PceI AGGCCT 1 cut(s) 133
PciSI GCTCTTC 1 cut(s) 197
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 1 cut(s) 148
Pfl23II CGTACG 1 cut(s) 24
PkrI GCNGC 5 cut(s) 32, 111, 126, 229, 293
Ppu21I YACGTR 1 cut(s) 158
PsiI TTATAA 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 230
PspGI CCWGG 1 cut(s) 230
PspLI CGTACG 1 cut(s) 24
PstNI CAGNNNCTG 1 cut(s) 91
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SacII CCGCGG 1 cut(s) 295
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 2 cut(s) 188, 258
SatI GCNGC 5 cut(s) 31, 110, 125, 228, 292
Sau3AI GATC 4 cut(s) 12, 55, 93, 298
ScrFI CCNGG 1 cut(s) 232
SetI ASST 5 cut(s) 160, 171, 220, 284, 341
Sfr303I CCGCGG 1 cut(s) 295
SfuI TTCGAA 1 cut(s) 137
SgrBI CCGCGG 1 cut(s) 295
SnaBI TACGTA 1 cut(s) 158
SphI GCATGC 1 cut(s) 211
Sse9I AATT 1 cut(s) 139
SseBI AGGCCT 1 cut(s) 133
SsiI CCGC 5 cut(s) 30, 124, 228, 292, 294
SspMI CTAG 1 cut(s) 215
StuI AGGCCT 1 cut(s) 133
StyD4I CCNGG 1 cut(s) 230
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 1 cut(s) 160
TaqI TCGA 3 cut(s) 38, 76, 137
TasI AATT 1 cut(s) 139
TauI GCSGC 4 cut(s) 33, 127, 230, 294
TfiI GAWTC 1 cut(s) 148
Tru1I TTAA 2 cut(s) 188, 258
Tru9I TTAA 2 cut(s) 188, 258
TseI GCWGC 1 cut(s) 109
XapI RAATTY 1 cut(s) 139
XceI RCATGY 1 cut(s) 211
XmnI GAANNNNTTC 1 cut(s) 133
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.