pycom05g31360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015228
Physical Location & Seq
Forward (+)
31113954 .. 31114295
342 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31360.1

Sequence Viewer

Length: 342 bp
ATGGCTATGATGATCAGAAAGTCGTACGGCGCCTACTCGAAGATGGACAAGGAAGATCCCGAAGAGGTAAATCATCGACGGGCGCAGTTCTTGATCTACAAAGTGATGCAACAAGTAGAAATGCGGCGGAGGCCTTCGAATTTGAGGATTCGGGTACGTAAACTGAAGCTGAAGATTGGGAGGAGATTAAAGAAGTTGAGGAAGAGCATGCTTCTAAGCGTATCTGCTGCCAGGGTTTGTGTTTATAAGCACGTTTTTAATCAATTAAAAACTTGCAAGACCTTGTTTGGCCGCGGAGATCAGGGAAATATCAACGTCACTCTTCCAGCTTTGTTCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

13.33

Weight (kDa)

11.05

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11655
fragaria_vesca FvH4_3g02270
malus_domestica MD00G1206700.v1.1 MD05G1344200.v1.1 MD10G1320200.v1.1
prunus_persica Prupe.4G022200_v2.0.a1
pyrus_communis pycom05g31360
rosa_chinensis RchiOBHm_Chr5g0003391
rosa_laevigata RLG00000031135
rosa_multiflora Rmu_co8199484.1_g000001 Rmu_sc0010917.1_g000001
rosa_roxburghii Rroxscaffold_1G00072630
rosa_rugosa Rorug04G0403000
rosa_samantha Rh5AG029600 Rh5CG031800
rosa_wichuraiana Rw5G002770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 29
AccII CGCG 1 cut(s) 294
AciI CCGC 4 cut(s) 124, 127, 292, 294
AclWI GGATC 1 cut(s) 50
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 1 cut(s) 139
AcuI CTGAAG 2 cut(s) 185, 191
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 2 cut(s) 26, 156
AjnI CCWGG 1 cut(s) 230
AluBI AGCT 2 cut(s) 169, 329
AluI AGCT 2 cut(s) 169, 329
AlwI GGATC 1 cut(s) 50
AoxI GGCC 2 cut(s) 131, 289
ApeKI GCWGC 1 cut(s) 227
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 2 cut(s) 32, 85
AsuHPI GGTGA 1 cut(s) 328
AsuII TTCGAA 1 cut(s) 137
BanI GGYRCC 1 cut(s) 29
BbvI GCAGC 1 cut(s) 214
BccI CCATC 1 cut(s) 37
BceAI ACGGC 1 cut(s) 43
BciT130I CCWGG 1 cut(s) 232
BclI TGATCA 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 33
BisI GCNGC 3 cut(s) 125, 228, 292
BlsI GCNGC 3 cut(s) 126, 229, 293
Bme1390I CCNGG 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 232
BmsI GCATC 1 cut(s) 96
Bpu14I TTCGAA 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 158
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 231, 292
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 2 cut(s) 231, 292
BseRI GAGGAG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 214
Bsh1236I CGCG 1 cut(s) 294
BshFI GGCC 2 cut(s) 133, 291
BshNI GGYRCC 1 cut(s) 29
BsiWI CGTACG 1 cut(s) 24
BsnI GGCC 2 cut(s) 133, 291
Bsp119I TTCGAA 1 cut(s) 137
Bsp143I GATC 4 cut(s) 12, 55, 93, 298
BspACI CCGC 4 cut(s) 124, 127, 292, 294
BspANI GGCC 2 cut(s) 133, 291
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 31
BspPI GGATC 1 cut(s) 50
BspQI GCTCTTC 1 cut(s) 197
BspT104I TTCGAA 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 29
BssECI CCNNGG 2 cut(s) 231, 292
BssMI GATC 4 cut(s) 12, 55, 93, 298
BssNI GRCGYC 1 cut(s) 30
Bst2UI CCWGG 1 cut(s) 232
Bst6I CTCTTC 3 cut(s) 57, 197, 327
BstACI GRCGYC 1 cut(s) 30
BstBAI YACGTR 1 cut(s) 158
BstBI TTCGAA 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 209
BstDEI CTNAG 1 cut(s) 215
BstDSI CCRYGG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 294
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 2 cut(s) 32, 85
BstKTI GATC 4 cut(s) 15, 58, 96, 301
BstMBI GATC 4 cut(s) 12, 55, 93, 298
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 230
BstSNI TACGTA 1 cut(s) 158
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BsuRI GGCC 2 cut(s) 133, 291
BtgI CCRYGG 1 cut(s) 292
Cac8I GCNNGC 1 cut(s) 209
CfoI GCGC 2 cut(s) 32, 85
Cfr42I CCGCGG 1 cut(s) 295
Csp6I GTAC 2 cut(s) 25, 155
CviAII CATG 1 cut(s) 208
CviJI RGCY 5 cut(s) 5, 133, 169, 291, 329
CviKI_1 RGCY 5 cut(s) 5, 133, 169, 291, 329
CviQI GTAC 2 cut(s) 25, 155
DdeI CTNAG 1 cut(s) 215
DinI GGCGCC 1 cut(s) 31
DpnI GATC 4 cut(s) 14, 57, 95, 300
DpnII GATC 4 cut(s) 12, 55, 93, 298
EaeI YGGCCR 1 cut(s) 289
Eam1104I CTCTTC 3 cut(s) 57, 197, 327
EarI CTCTTC 3 cut(s) 57, 197, 327
EciI GGCGGA 1 cut(s) 142
Eco105I TACGTA 1 cut(s) 158
Eco147I AGGCCT 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 185, 191
EcoRII CCWGG 1 cut(s) 230
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
FaeI CATG 1 cut(s) 211
FaiI YATR 3 cut(s) 8, 209, 246
FatI CATG 1 cut(s) 207
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 125, 228, 292
Fsp4HI GCNGC 3 cut(s) 125, 228, 292
GlaI GCGC 2 cut(s) 31, 84
GluI GCNGC 3 cut(s) 125, 228, 292
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 2 cut(s) 133, 291
HhaI GCGC 2 cut(s) 32, 85
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 1 cut(s) 211
Hin6I GCGC 2 cut(s) 30, 83
HinP1I GCGC 2 cut(s) 30, 83
HinfI GANTC 1 cut(s) 148
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 2 cut(s) 161, 336
Hpy188I TCNGA 1 cut(s) 17
Hpy188III TCNNGA 2 cut(s) 59, 91
Hpy8I GTNNAC 2 cut(s) 161, 336
Hpy99I CGWCG 1 cut(s) 81
HpyAV CCTTC 1 cut(s) 144
HpyCH4IV ACGT 3 cut(s) 157, 252, 315
HpyCH4V TGCA 2 cut(s) 109, 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 1 cut(s) 215
HpySE526I ACGT 3 cut(s) 157, 252, 315
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 1 cut(s) 211
HspAI GCGC 2 cut(s) 30, 83
KasI GGCGCC 1 cut(s) 29
Ksp22I TGATCA 1 cut(s) 12
KspI CCGCGG 1 cut(s) 295
Kzo9I GATC 4 cut(s) 12, 55, 93, 298
LguI GCTCTTC 1 cut(s) 197
LpnPI CCDG 3 cut(s) 217, 244, 287
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 1 cut(s) 96
MaeII ACGT 3 cut(s) 157, 252, 315
MaeIII GTNAC 1 cut(s) 316
MalI GATC 4 cut(s) 14, 57, 95, 300
MboI GATC 4 cut(s) 12, 55, 93, 298
MboII GAAGA 6 cut(s) 52, 65, 74, 184, 214, 314
MflI RGATCY 1 cut(s) 55
MluCI AATT 2 cut(s) 139, 263
Mly113I GGCGCC 1 cut(s) 30
MnlI CCTC 5 cut(s) 58, 123, 138, 174, 192
MseI TTAA 3 cut(s) 188, 258, 266
MspA1I CMGCKG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 232
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 1 cut(s) 130
NarI GGCGCC 1 cut(s) 30
NdeII GATC 4 cut(s) 12, 55, 93, 298
NlaIII CATG 1 cut(s) 211
NlaIV GGNNCC 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 316
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 1 cut(s) 137
PaeI GCATGC 1 cut(s) 211
PceI AGGCCT 1 cut(s) 133
PciSI GCTCTTC 1 cut(s) 197
PfeI GAWTC 1 cut(s) 148
Pfl23II CGTACG 1 cut(s) 24
PkrI GCNGC 3 cut(s) 126, 229, 293
PluTI GGCGCC 1 cut(s) 33
Ppu21I YACGTR 1 cut(s) 158
PsiI TTATAA 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 230
PspGI CCWGG 1 cut(s) 230
PspLI CGTACG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 31
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 2 cut(s) 26, 156
RsaNI GTAC 2 cut(s) 25, 155
SacII CCGCGG 1 cut(s) 295
SapI GCTCTTC 1 cut(s) 197
SaqAI TTAA 3 cut(s) 188, 258, 266
SatI GCNGC 3 cut(s) 125, 228, 292
Sau3AI GATC 4 cut(s) 12, 55, 93, 298
ScrFI CCNGG 1 cut(s) 232
SetI ASST 8 cut(s) 69, 160, 171, 255, 284, 318, 331, 341
SfaNI GCATC 1 cut(s) 96
SfoI GGCGCC 1 cut(s) 31
Sfr303I CCGCGG 1 cut(s) 295
SfuI TTCGAA 1 cut(s) 137
SgrBI CCGCGG 1 cut(s) 295
SnaBI TACGTA 1 cut(s) 158
SphI GCATGC 1 cut(s) 211
Sse9I AATT 2 cut(s) 139, 263
SseBI AGGCCT 1 cut(s) 133
SsiI CCGC 4 cut(s) 124, 127, 292, 294
SspDI GGCGCC 1 cut(s) 29
StuI AGGCCT 1 cut(s) 133
StyD4I CCNGG 1 cut(s) 230
TaiI ACGT 3 cut(s) 160, 255, 318
TaqI TCGA 3 cut(s) 38, 76, 137
TasI AATT 2 cut(s) 139, 263
TauI GCSGC 2 cut(s) 127, 294
TfiI GAWTC 1 cut(s) 148
Tru1I TTAA 3 cut(s) 188, 258, 266
Tru9I TTAA 3 cut(s) 188, 258, 266
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 1 cut(s) 227
Tsp45I GTSAC 1 cut(s) 316
XapI RAATTY 1 cut(s) 139
XceI RCATGY 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.