Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0016245

Physical Location & Seq

Genomic Coordinates Forward (+)

6903836 .. 6905850

Gene Structure 2015 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1014800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGGGTTGTGTTCAGGCGAAAACCAACTCCCCAAGTCATGACCTTCAAGTCCTTGACAAGTTGAAACGGGACAATGGCTATGTCAAGGGGAGCATTGAAGGAAAACCCGTTACCCTAAAACACTCAAGGAAGGACGTTAAGGCGGCGGCATTCAATGGTGAGGATAAGATTGCTGATGGTGGAGGAGGAGGTTTGGTTGTTAGAGAGACAGAGAGAGCTAAGAATGGAGGGAACGTTTCGCGAAGGATGATGTTGAAGAAGATTGGAGGAGATGATGATGATATTGTGGATGGCTGGCCGAAGTGGCTGGTTGAGAACGTGCCTCCAGATATCTTGGTTGGTTTTGTTCCAAAGACTGCCGACTCCTACGATAAACTTGCTAAGGTAGGGCAGGGAACCTACAGCAATGTGTACAAAGCTCGTGACAGGGACACCAAAAAGATTGTTGCTTTGAAAAAAGTCCGCTTTGACACATCAGAGCCAGAGAGTGTCAAGTTCATGGCAAGGGAGATAACGATGCTACAGAAGCTAGATCATCCCAATATCATCAAGCTTGAAGGGATAGCAACATCAAGAATGCAGTATAGTCTATATCTCGTATTTGATTTCATGCAGTCCGACTTGACCAGAGTAATCTCACGTCCTGGACAGAGGCTCACTGAACCCCAGGTCAAGTGCTATATGCAGCAGCTTCTTGCTGGTCTCCAGCACTGCCATGAAAACGGGATTTTACATCGAGACATTAAAGCTTCCAACTTGCTAATAGACAGAAATGGGATGCTGAAAATTGCAGATTTTGGGCTTGCAAATTTTTTCCCTCCAAAGAAACGTCCTCTTACAAGCCGAGTAGTGACACTCTGGTACAGAGCGCCAGAGCTCTTGTTAGGTTCTACGGATTATGGAGTTGGTATTGATCTTTGGAGTGCAGGATGCCTATTGGCAGAAATGTTTGTTGGGAGGCCAGTTATGCCTGGGAGAACCGAGGTTGAGCAACTTCATAGGATCTTCAAGCTTTGTGGTTCACCCGGAGAGGATTACTGGAAGAAAATGAAGTTACCAACAAGCTTCCGACCACCACAACATTACAAACCTAGTTTTGAAGAGTTCTTCAGGGACTTCTCAACATCATCATTTGATCTCTTAACAACACTTCTTGCTCTGGATCCGGCATCTCGTGGCAGTGCTGCTTCTGCACTCCAAAGTCAATTTTTTAGTTCAAGTCCGTCGGCATGTGACCTTTCAGCTCTACCAGTAATGTATAAAGAGGAGGACGCACGAACTCACACCAAGGACCGGAAGAAGTATGCTATTTCAAATTTCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

442

Amino Acids

49.44

Weight (kDa)

9.27

Isoelectric Point (pI)

41.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	123 - 404	2.4e-69	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	127 - 333	7.7e-34	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016245)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23160
malus_domestica	MD01G1014800.v1.1 MD15G1336200.v1.1
prunus_persica	Prupe.6G167000_v2.0.a1 Prupe.6G167000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0117881
rosa_laevigata	RLG00000018382
rosa_multiflora	Rmu_sc0004968.1_g000006
rosa_roxburghii	Rroxscaffold_2G00126130
rosa_rugosa	Rorug02G0214800
rosa_samantha	Rh2AG271000 Rh2BG282600 Rh2DG278900 Rh2DG296800
rosa_wichuraiana	Rw2G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	47
AccII	CGCG	1 cut(s)	241
AciI	CCGC	3 cut(s)	143, 146, 463
AclI	AACGTT	1 cut(s)	234
AclWI	GGATC	3 cut(s)	1010, 1157, 1170
AcoI	YGGCCR	1 cut(s)	296
AcsI	RAATTY	2 cut(s)	808, 1315
AcuI	CTGAAG	1 cut(s)	1093
AfaI	GTAC	2 cut(s)	413, 863
AfiI	CCNNNNNNNGG	1 cut(s)	1293
AjiI	CACGTC	1 cut(s)	641
AjnI	CCWGG	3 cut(s)	643, 666, 970
AjuI	GAANNNNNNNTTGG	2 cut(s)	936, 968
Alw21I	GWGCWC	1 cut(s)	879
Alw26I	GTCTC	3 cut(s)	200, 707, 732
AlwI	GGATC	3 cut(s)	1010, 1157, 1170
AoxI	GGCC	2 cut(s)	296, 959
ApeKI	GCWGC	3 cut(s)	685, 688, 1184
ApoI	RAATTY	2 cut(s)	808, 1315
AspLEI	GCGC	1 cut(s)	871
AspS9I	GGNCC	1 cut(s)	1291
AsuC2I	CCSGG	1 cut(s)	1026
AsuHPI	GGTGA	2 cut(s)	170, 1014
AvaII	GGWCC	1 cut(s)	1291
BamHI	GGATCC	1 cut(s)	1162
BanII	GRGCYC	1 cut(s)	879
BauI	CACGAG	2 cut(s)	420, 1173
Bbv12I	GWGCWC	1 cut(s)	879
BbvI	GCAGC	3 cut(s)	697, 700, 1171
BccI	CCATC	2 cut(s)	170, 284
BcgI	CGANNNNNNTGC	2 cut(s)	359, 393
BciT130I	CCWGG	3 cut(s)	645, 668, 972
BcnI	CCSGG	1 cut(s)	1026
BcoDI	GTCTC	3 cut(s)	200, 707, 732
BfaI	CTAG	2 cut(s)	530, 1092
BfmI	CTRYAG	2 cut(s)	400, 521
BfoI	RGCGCY	1 cut(s)	872
BglI	GCCNNNNNGGC	1 cut(s)	304
BisI	GCNGC	5 cut(s)	144, 147, 686, 689, 1185
BlsI	GCNGC	5 cut(s)	145, 148, 687, 690, 1186
Bme1390I	CCNGG	4 cut(s)	645, 668, 972, 1026
Bme18I	GGWCC	1 cut(s)	1291
BmgBI	CACGTC	1 cut(s)	641
BmgT120I	GGNCC	1 cut(s)	1291
BmiI	GGNNCC	2 cut(s)	397, 1164
BmrFI	CCNGG	4 cut(s)	645, 668, 972, 1026
BmsI	GCATC	4 cut(s)	507, 768, 920, 1178
BpmI	CTGGAG	2 cut(s)	309, 689
Bpu10I	CCTNAGC	1 cut(s)	381
BpuEI	CTTGAG	1 cut(s)	109
BpuMI	CCSGG	1 cut(s)	1026
BsaI	GGTCTC	1 cut(s)	707
BsaJI	CCNNGG	4 cut(s)	666, 971, 981, 1287
BsaWI	WCCGGW	1 cut(s)	1293
BsaXI	ACNNNNNCTCC	4 cut(s)	180, 210, 219, 249
Bsc4I	CCNNNNNNNGG	1 cut(s)	1293
Bse1I	ACTGG	3 cut(s)	962, 1043, 1250
Bse3DI	GCAATG	1 cut(s)	412
BseBI	CCWGG	3 cut(s)	645, 668, 972
BseDI	CCNNGG	4 cut(s)	666, 971, 981, 1287
BseGI	GGATG	5 cut(s)	252, 295, 535, 783, 935
BseLI	CCNNNNNNNGG	1 cut(s)	1293
BseMI	GCAATG	1 cut(s)	412
BseNI	ACTGG	3 cut(s)	962, 1043, 1250
BseRI	GAGGAG	4 cut(s)	198, 201, 282, 1280
BseXI	GCAGC	3 cut(s)	697, 700, 1171
BsgI	GTGCAG	2 cut(s)	945, 1176
Bsh1236I	CGCG	1 cut(s)	241
BshFI	GGCC	2 cut(s)	298, 961
BsiHKAI	GWGCWC	1 cut(s)	879
BsiSI	CCGG	3 cut(s)	1026, 1166, 1294
BslFI	GGGAC	3 cut(s)	83, 443, 1127
BslI	CCNNNNNNNGG	1 cut(s)	1293
BsmAI	GTCTC	3 cut(s)	200, 707, 732
BsmFI	GGGAC	3 cut(s)	83, 443, 1127
BsmI	GAATGC	2 cut(s)	149, 582
BsnI	GGCC	2 cut(s)	298, 961
Bso31I	GGTCTC	1 cut(s)	707
Bsp1286I	GDGCHC	1 cut(s)	879
Bsp1407I	TGTACA	1 cut(s)	411
Bsp143I	GATC	5 cut(s)	532, 913, 1002, 1135, 1162
Bsp68I	TCGCGA	1 cut(s)	241
BspACI	CCGC	3 cut(s)	143, 146, 463
BspANI	GGCC	2 cut(s)	298, 961
BspFNI	CGCG	1 cut(s)	241
BspHI	TCATGA	1 cut(s)	37
BspLI	GGNNCC	2 cut(s)	397, 1164
BspPI	GGATC	3 cut(s)	1010, 1157, 1170
BspTNI	GGTCTC	1 cut(s)	707
BsrDI	GCAATG	1 cut(s)	412
BsrGI	TGTACA	1 cut(s)	411
BsrI	ACTGG	3 cut(s)	962, 1043, 1250
BssECI	CCNNGG	4 cut(s)	666, 971, 981, 1287
BssMI	GATC	5 cut(s)	532, 913, 1002, 1135, 1162
BssSI	CACGAG	2 cut(s)	420, 1173
BssT1I	CCWWGG	1 cut(s)	1287
Bst2BI	CACGAG	2 cut(s)	420, 1173
Bst2UI	CCWGG	3 cut(s)	645, 668, 972
Bst6I	CTCTTC	1 cut(s)	1095
BstAUI	TGTACA	1 cut(s)	411
BstC8I	GCNNGC	2 cut(s)	296, 804
BstDEI	CTNAG	2 cut(s)	219, 381
BstF5I	GGATG	5 cut(s)	252, 295, 535, 783, 935
BstFNI	CGCG	1 cut(s)	241
BstH2I	RGCGCY	1 cut(s)	872
BstHHI	GCGC	1 cut(s)	871
BstKTI	GATC	5 cut(s)	535, 916, 1005, 1138, 1165
BstMAI	GTCTC	3 cut(s)	200, 707, 732
BstMBI	GATC	5 cut(s)	532, 913, 1002, 1135, 1162
BstMWI	GCNNNNNNNGC	4 cut(s)	304, 526, 967, 1190
BstNI	CCWGG	3 cut(s)	645, 668, 972
BstNSI	RCATGY	1 cut(s)	1233
BstSCI	CCNGG	4 cut(s)	643, 666, 970, 1024
BstSFI	CTRYAG	2 cut(s)	400, 521
BstUI	CGCG	1 cut(s)	241
BstV1I	GCAGC	3 cut(s)	697, 700, 1171
BstX2I	RGATCY	2 cut(s)	1002, 1162
BstYI	RGATCY	2 cut(s)	1002, 1162
BsuRI	GGCC	2 cut(s)	298, 961
BtrI	CACGTC	1 cut(s)	641
BtsCI	GGATG	5 cut(s)	252, 295, 535, 783, 935
BtsI	GCAGTG	2 cut(s)	709, 1186
BtsIMutI	CAGTG	3 cut(s)	657, 709, 1186
BtuMI	TCGCGA	1 cut(s)	241
Cac8I	GCNNGC	2 cut(s)	296, 804
CciI	TCATGA	1 cut(s)	37
CfoI	GCGC	1 cut(s)	871
Cfr13I	GGNCC	1 cut(s)	1291
CseI	GACGC	1 cut(s)	1280
Csp6I	GTAC	2 cut(s)	412, 862
CspCI	CAANNNNNGTGG	2 cut(s)	997, 1032
CviAII	CATG	5 cut(s)	38, 499, 610, 716, 1230
CviQI	GTAC	2 cut(s)	412, 862
DdeI	CTNAG	2 cut(s)	219, 381
DpnI	GATC	5 cut(s)	534, 915, 1004, 1137, 1164
DpnII	GATC	5 cut(s)	532, 913, 1002, 1135, 1162
DrdI	GACNNNNNNGTC	1 cut(s)	47
DseDI	GACNNNNNNGTC	1 cut(s)	47
EaeI	YGGCCR	1 cut(s)	296
Eam1104I	CTCTTC	1 cut(s)	1095
EarI	CTCTTC	1 cut(s)	1095
Ecl136II	GAGCTC	1 cut(s)	877
Eco130I	CCWWGG	1 cut(s)	1287
Eco24I	GRGCYC	1 cut(s)	879
Eco31I	GGTCTC	1 cut(s)	707
Eco32I	GATATC	1 cut(s)	331
Eco47I	GGWCC	1 cut(s)	1291
Eco53kI	GAGCTC	1 cut(s)	877
Eco57I	CTGAAG	1 cut(s)	1093
EcoICRI	GAGCTC	1 cut(s)	877
EcoRII	CCWGG	3 cut(s)	643, 666, 970
EcoRV	GATATC	1 cut(s)	331
EcoT14I	CCWWGG	1 cut(s)	1287
EcoT38I	GRGCYC	1 cut(s)	879
ErhI	CCWWGG	1 cut(s)	1287
FaeI	CATG	5 cut(s)	41, 502, 613, 719, 1233
FaqI	GGGAC	3 cut(s)	83, 443, 1127
FatI	CATG	5 cut(s)	37, 498, 609, 715, 1229
Fnu4HI	GCNGC	5 cut(s)	144, 147, 686, 689, 1185
FokI	GGATG	5 cut(s)	259, 302, 522, 790, 942
FriOI	GRGCYC	1 cut(s)	879
Fsp4HI	GCNGC	5 cut(s)	144, 147, 686, 689, 1185
FspBI	CTAG	2 cut(s)	530, 1092
GlaI	GCGC	1 cut(s)	870
GluI	GCNGC	5 cut(s)	144, 147, 686, 689, 1185
GsuI	CTGGAG	2 cut(s)	309, 689
HaeII	RGCGCY	1 cut(s)	872
HaeIII	GGCC	2 cut(s)	298, 961
HapII	CCGG	3 cut(s)	1026, 1166, 1294
HgaI	GACGC	1 cut(s)	1280
HhaI	GCGC	1 cut(s)	871
Hin1II	CATG	5 cut(s)	41, 502, 613, 719, 1233
Hin6I	GCGC	1 cut(s)	869
HinP1I	GCGC	1 cut(s)	869
HindIII	AAGCTT	4 cut(s)	551, 747, 1010, 1063
HinfI	GANTC	1 cut(s)	362
HpaII	CCGG	3 cut(s)	1026, 1166, 1294
HphI	GGTGA	2 cut(s)	170, 1014
Hpy166II	GTNNAC	2 cut(s)	412, 1022
Hpy188I	TCNGA	3 cut(s)	478, 619, 1070
Hpy188III	TCNNGA	7 cut(s)	38, 240, 326, 422, 573, 737, 1160
Hpy8I	GTNNAC	2 cut(s)	412, 1022
Hpy99I	CGWCG	1 cut(s)	1228
HpyAV	CCTTC	5 cut(s)	53, 92, 124, 237, 551
HpyCH4IV	ACGT	5 cut(s)	135, 234, 318, 640, 829
HpyCH4V	TGCA	7 cut(s)	580, 613, 685, 791, 806, 926, 1193
HpyF10VI	GCNNNNNNNGC	4 cut(s)	304, 526, 967, 1190
HpyF3I	CTNAG	2 cut(s)	219, 381
HpySE526I	ACGT	5 cut(s)	135, 234, 318, 640, 829
Hsp92II	CATG	5 cut(s)	41, 502, 613, 719, 1233
HspAI	GCGC	1 cut(s)	869
Kzo9I	GATC	5 cut(s)	532, 913, 1002, 1135, 1162
LmnI	GCTCC	1 cut(s)	90
Lsp1109I	GCAGC	3 cut(s)	697, 700, 1171
LweI	GCATC	4 cut(s)	507, 768, 920, 1178
MaeI	CTAG	2 cut(s)	530, 1092
MaeII	ACGT	5 cut(s)	135, 234, 318, 640, 829
MaeIII	GTNAC	5 cut(s)	109, 422, 850, 1053, 1232
MalI	GATC	5 cut(s)	534, 915, 1004, 1137, 1164
MboI	GATC	5 cut(s)	532, 913, 1002, 1135, 1162
MboII	GAAGA	7 cut(s)	268, 271, 997, 1054, 1099, 1112, 1309
MflI	RGATCY	2 cut(s)	1002, 1162
MhlI	GDGCHC	1 cut(s)	879
MluCI	AATT	4 cut(s)	786, 808, 1205, 1315
MlyI	GAGTC	1 cut(s)	356
MmeI	TCCRAC	3 cut(s)	642, 777, 1093
MseI	TTAA	3 cut(s)	138, 744, 1142
MslI	CAYNNNNRTG	1 cut(s)	714
MspI	CCGG	3 cut(s)	1026, 1166, 1294
MspR9I	CCNGG	4 cut(s)	645, 668, 972, 1026
Mva1269I	GAATGC	2 cut(s)	149, 582
MvaI	CCWGG	3 cut(s)	645, 668, 972
MvnI	CGCG	1 cut(s)	241
MwoI	GCNNNNNNNGC	4 cut(s)	304, 526, 967, 1190
NciI	CCSGG	1 cut(s)	1026
NdeII	GATC	5 cut(s)	532, 913, 1002, 1135, 1162
NlaIII	CATG	5 cut(s)	41, 502, 613, 719, 1233
NlaIV	GGNNCC	2 cut(s)	397, 1164
NmeAIII	GCCGAG	1 cut(s)	869
NmuCI	GTSAC	3 cut(s)	422, 850, 1232
NruI	TCGCGA	1 cut(s)	241
NspI	RCATGY	1 cut(s)	1233
PagI	TCATGA	1 cut(s)	37
PctI	GAATGC	2 cut(s)	149, 582
PfoI	TCCNGGA	1 cut(s)	643
PkrI	GCNGC	5 cut(s)	145, 148, 687, 690, 1186
PleI	GAGTC	1 cut(s)	356
PpsI	GAGTC	1 cut(s)	356
Psp124BI	GAGCTC	1 cut(s)	879
Psp1406I	AACGTT	1 cut(s)	234
Psp6I	CCWGG	3 cut(s)	643, 666, 970
PspGI	CCWGG	3 cut(s)	643, 666, 970
PspN4I	GGNNCC	2 cut(s)	397, 1164
PspPI	GGNCC	1 cut(s)	1291
PsuI	RGATCY	2 cut(s)	1002, 1162
RruI	TCGCGA	1 cut(s)	241
RsaI	GTAC	2 cut(s)	413, 863
RsaNI	GTAC	2 cut(s)	412, 862
RseI	CAYNNNNRTG	1 cut(s)	714
SacI	GAGCTC	1 cut(s)	879
SaqAI	TTAA	3 cut(s)	138, 744, 1142
SatI	GCNGC	5 cut(s)	144, 147, 686, 689, 1185
Sau3AI	GATC	5 cut(s)	532, 913, 1002, 1135, 1162
Sau96I	GGNCC	1 cut(s)	1291
SchI	GAGTC	1 cut(s)	356
ScrFI	CCNGG	4 cut(s)	645, 668, 972, 1026
SduI	GDGCHC	1 cut(s)	879
SfaNI	GCATC	4 cut(s)	507, 768, 920, 1178
SfcI	CTRYAG	2 cut(s)	400, 521
SinI	GGWCC	1 cut(s)	1291
SmiMI	CAYNNNNRTG	1 cut(s)	714
SmlI	CTYRAG	1 cut(s)	124
SmoI	CTYRAG	1 cut(s)	124
Sse9I	AATT	4 cut(s)	786, 808, 1205, 1315
SsiI	CCGC	3 cut(s)	143, 146, 463
SspMI	CTAG	2 cut(s)	530, 1092
SstI	GAGCTC	1 cut(s)	879
StyD4I	CCNGG	4 cut(s)	643, 666, 970, 1024
StyI	CCWWGG	1 cut(s)	1287
TaiI	ACGT	5 cut(s)	138, 237, 321, 643, 832
TaqI	TCGA	1 cut(s)	736
TasI	AATT	4 cut(s)	786, 808, 1205, 1315
TatI	WGTACW	1 cut(s)	411
TauI	GCSGC	2 cut(s)	146, 149
Tru1I	TTAA	3 cut(s)	138, 744, 1142
Tru9I	TTAA	3 cut(s)	138, 744, 1142
TscAI	CASTG	3 cut(s)	664, 716, 1186
TseFI	GTSAC	3 cut(s)	422, 850, 1232
TseI	GCWGC	3 cut(s)	685, 688, 1184
Tsp45I	GTSAC	3 cut(s)	422, 850, 1232
TspDTI	ATGAA	5 cut(s)	487, 598, 732, 986, 1064
TspGWI	ACGGA	2 cut(s)	908, 1212
TspRI	CASTG	3 cut(s)	664, 716, 1186
VpaK11BI	GGWCC	1 cut(s)	1291
XapI	RAATTY	2 cut(s)	808, 1315
XceI	RCATGY	1 cut(s)	1233
XspI	CTAG	2 cut(s)	530, 1092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.