Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0016245

Physical Location & Seq

Genomic Coordinates Reverse (-)

37646735 .. 37648715

Gene Structure 1981 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1336200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGGGTTGTGTTCAGACGAAAACTAACTCCCCAAGTCATAACCTTCAAGTCCTTGACAAGTTGAAATGGGACAATGGCTATGTCAAGGGGAGCACTGGAGGAAGACCCGTTACCCAAAAACACTCGAGGAAGGAGATTAAGCCGGTGTTCAATGGGGAGGATAAGATTGCTGATGGTGGTAGAACCGGTGGAGCAGGAGGAGGAGGAGGAGGAGGAGGAGGAGAAGGTTTGGTTGTTCGAGAGAAAGAGAGAGGTAAGAACGGTGGGAATGTTTCGGGAAGGATGACGTTGGAGAAGATTGGAGGAGATGATGATATTGTGAATGGCTGGCCAAAGTGGTTGGTTGAGAATGTGCCTCAAGATGTCTTGGCTGGTTTCGTTCCAAAGACTGCGGACTCCTATGATAAACTTGCTAAGATAGGGCAGGGAACCTACAGCAATGTGTACAAAGCTCGTGACAGGGACACCAAAAAGATTGTCGCTTTGAAAAAAGTCCGGTTTGACACATCAGAGCCAGAGAGTGTCAAGTTTATGGCGAGGGAGATAACGATGCTACAGAAGCTAGATCATCCCAATATCATCAAGCTTGAAGGGGTAGCTACATCAAGAATGCAGTATAGTCTATATCTCGTCTTTGATGTCATGCAGTCCGACTTGACTAGAGTAATCTCACGTCCTGGAAAGAGGCTCACTGAACCCCAGGTCAAGTGCTATATGCAGCAGCTTCTTGCTGGTCTCCAGCACTGCCATGAAAACGGGATTTTACACCGAGACATTAAAGCTTCCAACTTGCTAATAGACAGAAATGGGATGTTGAAAATTGCAGATTTTGGGCTTGCAAATTTTTTCCCTCCTAAGAAACGTCCTCTTACAAGCCGAGTTGTGACACTCTGGTACAGAGCGCCAGAGCTCTTGTTAGGTTCTACAGATTATGGAGTTGGTATTGATCTTTGGAGTGCAGGATGCCTGTTGGCAGAGATGTTTGTTGGGAGGCCAGTTATGCCTGGGAGGACCGAGGTTGAGCAACTTCATAAGATCTTCAAGCTTTGTGGTTTACCCGGAGAGGATTACTGGAAGAAAATGAAGTTACCAACAAGCTTCCGACCACCACAACATTACAAACCTAGTTATGAAGAATTCTTCAGGGACTTCTCAAGATCATCATTTGATCTCTTAACAACACTTCTTGCTCTGGACCCGGCATCTCGTGGCAGTGCTGCTTCTGCTCTCCAAAGTCAATTTTTTAGTTCGAGTCCGTTGGCATGTGACCTTTCAGCTCTACCAGTAATGTATAAAGAGGAGGACGAACGCACTCACGCCAAGGATCGGAAGAAGTATGCTATTTCACATTTCAATTATACATTGCAATTTACTTTCGTTCACTATTTTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

465

Amino Acids

51.85

Weight (kDa)

9.29

Isoelectric Point (pI)

43.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	134 - 415	9.2e-70	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	138 - 347	7.9e-34	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016245)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23160
malus_domestica	MD01G1014800.v1.1 MD15G1336200.v1.1
prunus_persica	Prupe.6G167000_v2.0.a1 Prupe.6G167000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0117881
rosa_laevigata	RLG00000018382
rosa_multiflora	Rmu_sc0004968.1_g000006
rosa_roxburghii	Rroxscaffold_2G00126130
rosa_rugosa	Rorug02G0214800
rosa_samantha	Rh2AG271000 Rh2BG282600 Rh2DG278900 Rh2DG296800
rosa_wichuraiana	Rw2G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	392
AclWI	GGATC	1 cut(s)	1332
AcoI	YGGCCR	1 cut(s)	329
AcsI	RAATTY	2 cut(s)	841, 1136
AcuI	CTGAAG	1 cut(s)	1126
AfaI	GTAC	2 cut(s)	446, 896
AfiI	CCNNNNNNNGG	1 cut(s)	1326
AgeI	ACCGGT	1 cut(s)	185
AgsI	TTSAA	8 cut(s)	47, 64, 151, 487, 590, 817, 1042, 1354
AjiI	CACGTC	1 cut(s)	674
AjnI	CCWGG	3 cut(s)	676, 699, 1003
Alw21I	GWGCWC	2 cut(s)	95, 912
Alw26I	GTCTC	2 cut(s)	740, 765
AlwI	GGATC	1 cut(s)	1332
Ama87I	CYCGRG	1 cut(s)	124
AoxI	GGCC	2 cut(s)	329, 992
ApeKI	GCWGC	3 cut(s)	718, 721, 1217
ApoI	RAATTY	2 cut(s)	841, 1136
ArsI	GACNNNNNNTTYG	2 cut(s)	462, 494
AsiGI	ACCGGT	1 cut(s)	185
AspLEI	GCGC	1 cut(s)	904
AspS9I	GGNCC	2 cut(s)	1011, 1195
AsuC2I	CCSGG	2 cut(s)	1059, 1199
AvaI	CYCGRG	1 cut(s)	124
AvaII	GGWCC	2 cut(s)	1011, 1195
BalI	TGGCCA	1 cut(s)	331
BanII	GRGCYC	1 cut(s)	912
BarI	GAAGNNNNNNTAC	2 cut(s)	94, 126
BauI	CACGAG	2 cut(s)	453, 1206
BbsI	GAAGAC	1 cut(s)	109
Bbv12I	GWGCWC	2 cut(s)	95, 912
BbvI	GCAGC	3 cut(s)	730, 733, 1204
BccI	CCATC	1 cut(s)	167
BciT130I	CCWGG	3 cut(s)	678, 701, 1005
BcnI	CCSGG	2 cut(s)	1059, 1199
BcoDI	GTCTC	2 cut(s)	740, 765
BfaI	CTAG	3 cut(s)	563, 660, 1125
BfmI	CTRYAG	3 cut(s)	433, 554, 924
BfoI	RGCGCY	1 cut(s)	905
BglII	AGATCT	1 cut(s)	1035
BisI	GCNGC	3 cut(s)	719, 722, 1218
BlsI	GCNGC	3 cut(s)	720, 723, 1219
Bme1390I	CCNGG	5 cut(s)	678, 701, 1005, 1059, 1199
Bme18I	GGWCC	2 cut(s)	1011, 1195
BmeT110I	CYCGRG	1 cut(s)	124
BmgBI	CACGTC	1 cut(s)	674
BmgT120I	GGNCC	2 cut(s)	1011, 1195
BmiI	GGNNCC	2 cut(s)	430, 1197
BmrFI	CCNGG	5 cut(s)	678, 701, 1005, 1059, 1199
BmsI	GCATC	3 cut(s)	540, 953, 1211
BpiI	GAAGAC	1 cut(s)	109
BpmI	CTGGAG	2 cut(s)	117, 722
BpuEI	CTTGAG	2 cut(s)	342, 1138
BpuMI	CCSGG	2 cut(s)	1059, 1199
BsaI	GGTCTC	1 cut(s)	740
BsaJI	CCNNGG	4 cut(s)	699, 1004, 1014, 1320
BsaWI	WCCGGW	2 cut(s)	185, 495
Bsc4I	CCNNNNNNNGG	1 cut(s)	1326
Bse118I	RCCGGY	2 cut(s)	142, 185
Bse1I	ACTGG	4 cut(s)	100, 995, 1076, 1283
Bse3DI	GCAATG	2 cut(s)	445, 1361
BseBI	CCWGG	3 cut(s)	678, 701, 1005
BseDI	CCNNGG	4 cut(s)	699, 1004, 1014, 1320
BseGI	GGATG	4 cut(s)	288, 568, 816, 968
BseLI	CCNNNNNNNGG	1 cut(s)	1326
BseMI	GCAATG	2 cut(s)	445, 1361
BseNI	ACTGG	4 cut(s)	100, 995, 1076, 1283
BseXI	GCAGC	3 cut(s)	730, 733, 1204
BsgI	GTGCAG	1 cut(s)	978
BshFI	GGCC	2 cut(s)	331, 994
BshTI	ACCGGT	1 cut(s)	185
BsiHKAI	GWGCWC	2 cut(s)	95, 912
BsiHKCI	CYCGRG	1 cut(s)	124
BsiSI	CCGG	5 cut(s)	143, 186, 496, 1059, 1199
BslFI	GGGAC	3 cut(s)	83, 476, 1160
BslI	CCNNNNNNNGG	1 cut(s)	1326
BsmAI	GTCTC	2 cut(s)	740, 765
BsmFI	GGGAC	3 cut(s)	83, 476, 1160
BsmI	GAATGC	1 cut(s)	615
BsnI	GGCC	2 cut(s)	331, 994
Bso31I	GGTCTC	1 cut(s)	740
BsoBI	CYCGRG	1 cut(s)	124
Bsp1286I	GDGCHC	2 cut(s)	95, 912
Bsp1407I	TGTACA	1 cut(s)	444
Bsp143I	GATC	6 cut(s)	565, 946, 1035, 1157, 1168, 1324
BspACI	CCGC	1 cut(s)	392
BspANI	GGCC	2 cut(s)	331, 994
BspLI	GGNNCC	2 cut(s)	430, 1197
BspPI	GGATC	1 cut(s)	1332
BspTNI	GGTCTC	1 cut(s)	740
BsrDI	GCAATG	2 cut(s)	445, 1361
BsrFI	RCCGGY	2 cut(s)	142, 185
BsrGI	TGTACA	1 cut(s)	444
BsrI	ACTGG	4 cut(s)	100, 995, 1076, 1283
BssAI	RCCGGY	2 cut(s)	142, 185
BssECI	CCNNGG	4 cut(s)	699, 1004, 1014, 1320
BssMI	GATC	6 cut(s)	565, 946, 1035, 1157, 1168, 1324
BssSI	CACGAG	2 cut(s)	453, 1206
BssT1I	CCWWGG	1 cut(s)	1320
Bst2BI	CACGAG	2 cut(s)	453, 1206
Bst2UI	CCWGG	3 cut(s)	678, 701, 1005
Bst4CI	ACNGT	1 cut(s)	263
BstAUI	TGTACA	1 cut(s)	444
BstC8I	GCNNGC	2 cut(s)	329, 837
BstDEI	CTNAG	2 cut(s)	414, 855
BstF5I	GGATG	4 cut(s)	288, 568, 816, 968
BstH2I	RGCGCY	1 cut(s)	905
BstHHI	GCGC	1 cut(s)	904
BstKTI	GATC	6 cut(s)	568, 949, 1038, 1160, 1171, 1327
BstMAI	GTCTC	2 cut(s)	740, 765
BstMBI	GATC	6 cut(s)	565, 946, 1035, 1157, 1168, 1324
BstMWI	GCNNNNNNNGC	3 cut(s)	559, 1000, 1223
BstNI	CCWGG	3 cut(s)	678, 701, 1005
BstNSI	RCATGY	1 cut(s)	1266
BstSCI	CCNGG	5 cut(s)	676, 699, 1003, 1057, 1197
BstSFI	CTRYAG	3 cut(s)	433, 554, 924
BstV1I	GCAGC	3 cut(s)	730, 733, 1204
BstV2I	GAAGAC	1 cut(s)	109
BstX2I	RGATCY	1 cut(s)	1035
BstYI	RGATCY	1 cut(s)	1035
BsuRI	GGCC	2 cut(s)	331, 994
BtrI	CACGTC	1 cut(s)	674
BtsCI	GGATG	4 cut(s)	288, 568, 816, 968
BtsI	GCAGTG	2 cut(s)	742, 1219
BtsIMutI	CAGTG	4 cut(s)	93, 690, 742, 1219
Cac8I	GCNNGC	2 cut(s)	329, 837
CfoI	GCGC	1 cut(s)	904
Cfr10I	RCCGGY	2 cut(s)	142, 185
Cfr13I	GGNCC	2 cut(s)	1011, 1195
Csp6I	GTAC	2 cut(s)	445, 895
CspAI	ACCGGT	1 cut(s)	185
CspCI	CAANNNNNGTGG	2 cut(s)	1030, 1065
CviAII	CATG	3 cut(s)	643, 749, 1263
CviQI	GTAC	2 cut(s)	445, 895
DdeI	CTNAG	2 cut(s)	414, 855
DpnI	GATC	6 cut(s)	567, 948, 1037, 1159, 1170, 1326
DpnII	GATC	6 cut(s)	565, 946, 1035, 1157, 1168, 1324
EaeI	YGGCCR	1 cut(s)	329
Ecl136II	GAGCTC	1 cut(s)	910
Eco130I	CCWWGG	1 cut(s)	1320
Eco24I	GRGCYC	1 cut(s)	912
Eco31I	GGTCTC	1 cut(s)	740
Eco47I	GGWCC	2 cut(s)	1011, 1195
Eco53kI	GAGCTC	1 cut(s)	910
Eco57I	CTGAAG	1 cut(s)	1126
Eco88I	CYCGRG	1 cut(s)	124
EcoICRI	GAGCTC	1 cut(s)	910
EcoRI	GAATTC	1 cut(s)	1136
EcoRII	CCWGG	3 cut(s)	676, 699, 1003
EcoT14I	CCWWGG	1 cut(s)	1320
EcoT38I	GRGCYC	1 cut(s)	912
ErhI	CCWWGG	1 cut(s)	1320
FaeI	CATG	3 cut(s)	646, 752, 1266
FaqI	GGGAC	3 cut(s)	83, 476, 1160
FatI	CATG	3 cut(s)	642, 748, 1262
Fnu4HI	GCNGC	3 cut(s)	719, 722, 1218
FokI	GGATG	4 cut(s)	295, 555, 823, 975
FriOI	GRGCYC	1 cut(s)	912
Fsp4HI	GCNGC	3 cut(s)	719, 722, 1218
FspBI	CTAG	3 cut(s)	563, 660, 1125
GlaI	GCGC	1 cut(s)	903
GluI	GCNGC	3 cut(s)	719, 722, 1218
GsuI	CTGGAG	2 cut(s)	117, 722
HaeII	RGCGCY	1 cut(s)	905
HaeIII	GGCC	2 cut(s)	331, 994
HapII	CCGG	5 cut(s)	143, 186, 496, 1059, 1199
HhaI	GCGC	1 cut(s)	904
Hin1II	CATG	3 cut(s)	646, 752, 1266
Hin6I	GCGC	1 cut(s)	902
HinP1I	GCGC	1 cut(s)	902
HindIII	AAGCTT	4 cut(s)	584, 780, 1043, 1096
HinfI	GANTC	2 cut(s)	395, 1252
HpaII	CCGG	5 cut(s)	143, 186, 496, 1059, 1199
Hpy166II	GTNNAC	3 cut(s)	445, 1055, 1381
Hpy188I	TCNGA	5 cut(s)	15, 511, 652, 1103, 1329
Hpy188III	TCNNGA	7 cut(s)	239, 276, 359, 455, 606, 1155, 1193
Hpy8I	GTNNAC	3 cut(s)	445, 1055, 1381
HpyAV	CCTTC	5 cut(s)	53, 124, 218, 273, 584
HpyCH4III	ACNGT	1 cut(s)	263
HpyCH4IV	ACGT	3 cut(s)	287, 673, 862
HpyCH4V	TGCA	7 cut(s)	613, 646, 718, 824, 839, 959, 1366
HpyF10VI	GCNNNNNNNGC	3 cut(s)	559, 1000, 1223
HpyF3I	CTNAG	2 cut(s)	414, 855
HpySE526I	ACGT	3 cut(s)	287, 673, 862
Hsp92II	CATG	3 cut(s)	646, 752, 1266
HspAI	GCGC	1 cut(s)	902
Kzo9I	GATC	6 cut(s)	565, 946, 1035, 1157, 1168, 1324
LmnI	GCTCC	2 cut(s)	90, 191
Lsp1109I	GCAGC	3 cut(s)	730, 733, 1204
LweI	GCATC	3 cut(s)	540, 953, 1211
MaeI	CTAG	3 cut(s)	563, 660, 1125
MaeII	ACGT	3 cut(s)	287, 673, 862
MaeIII	GTNAC	5 cut(s)	109, 455, 883, 1086, 1265
MalI	GATC	6 cut(s)	567, 948, 1037, 1159, 1170, 1326
MboI	GATC	6 cut(s)	565, 946, 1035, 1157, 1168, 1324
MboII	GAAGA	7 cut(s)	114, 307, 1030, 1087, 1132, 1145, 1342
MflI	RGATCY	1 cut(s)	1035
MhlI	GDGCHC	2 cut(s)	95, 912
MlsI	TGGCCA	1 cut(s)	331
MluCI	AATT	6 cut(s)	819, 841, 1136, 1238, 1354, 1367
MluNI	TGGCCA	1 cut(s)	331
MlyI	GAGTC	2 cut(s)	389, 1261
MmeI	TCCRAC	4 cut(s)	270, 675, 810, 1126
Mox20I	TGGCCA	1 cut(s)	331
MscI	TGGCCA	1 cut(s)	331
MseI	TTAA	4 cut(s)	138, 777, 1175, 1393
MslI	CAYNNNNRTG	1 cut(s)	747
Msp20I	TGGCCA	1 cut(s)	331
MspI	CCGG	5 cut(s)	143, 186, 496, 1059, 1199
MspR9I	CCNGG	5 cut(s)	678, 701, 1005, 1059, 1199
Mva1269I	GAATGC	1 cut(s)	615
MvaI	CCWGG	3 cut(s)	678, 701, 1005
MwoI	GCNNNNNNNGC	3 cut(s)	559, 1000, 1223
NciI	CCSGG	2 cut(s)	1059, 1199
NdeII	GATC	6 cut(s)	565, 946, 1035, 1157, 1168, 1324
NlaIII	CATG	3 cut(s)	646, 752, 1266
NlaIV	GGNNCC	2 cut(s)	430, 1197
NmeAIII	GCCGAG	1 cut(s)	902
NmuCI	GTSAC	3 cut(s)	455, 883, 1265
NspI	RCATGY	1 cut(s)	1266
PaeR7I	CTCGAG	1 cut(s)	124
PctI	GAATGC	1 cut(s)	615
PfoI	TCCNGGA	1 cut(s)	676
PinAI	ACCGGT	1 cut(s)	185
PkrI	GCNGC	3 cut(s)	720, 723, 1219
PleI	GAGTC	2 cut(s)	389, 1260
PpsI	GAGTC	2 cut(s)	389, 1260
Psp124BI	GAGCTC	1 cut(s)	912
Psp6I	CCWGG	3 cut(s)	676, 699, 1003
PspGI	CCWGG	3 cut(s)	676, 699, 1003
PspN4I	GGNNCC	2 cut(s)	430, 1197
PspPI	GGNCC	2 cut(s)	1011, 1195
PspXI	VCTCGAGB	1 cut(s)	124
PsuI	RGATCY	1 cut(s)	1035
RsaI	GTAC	2 cut(s)	446, 896
RsaNI	GTAC	2 cut(s)	445, 895
RseI	CAYNNNNRTG	1 cut(s)	747
SacI	GAGCTC	1 cut(s)	912
SaqAI	TTAA	4 cut(s)	138, 777, 1175, 1393
SatI	GCNGC	3 cut(s)	719, 722, 1218
Sau3AI	GATC	6 cut(s)	565, 946, 1035, 1157, 1168, 1324
Sau96I	GGNCC	2 cut(s)	1011, 1195
SchI	GAGTC	2 cut(s)	389, 1261
ScrFI	CCNGG	5 cut(s)	678, 701, 1005, 1059, 1199
SduI	GDGCHC	2 cut(s)	95, 912
SfaNI	GCATC	3 cut(s)	540, 953, 1211
SfcI	CTRYAG	3 cut(s)	433, 554, 924
Sfr274I	CTCGAG	1 cut(s)	124
SinI	GGWCC	2 cut(s)	1011, 1195
SlaI	CTCGAG	1 cut(s)	124
SmiMI	CAYNNNNRTG	1 cut(s)	747
SmlI	CTYRAG	3 cut(s)	124, 357, 1153
SmoI	CTYRAG	3 cut(s)	124, 357, 1153
Sse9I	AATT	6 cut(s)	819, 841, 1136, 1238, 1354, 1367
SsiI	CCGC	1 cut(s)	392
SspMI	CTAG	3 cut(s)	563, 660, 1125
SstI	GAGCTC	1 cut(s)	912
StyD4I	CCNGG	5 cut(s)	676, 699, 1003, 1057, 1197
StyI	CCWWGG	1 cut(s)	1320
TaaI	ACNGT	1 cut(s)	263
TaiI	ACGT	3 cut(s)	290, 676, 865
TaqI	TCGA	3 cut(s)	125, 238, 1250
TaqII	GACCGA	1 cut(s)	1028
TasI	AATT	6 cut(s)	819, 841, 1136, 1238, 1354, 1367
TatI	WGTACW	1 cut(s)	444
Tru1I	TTAA	4 cut(s)	138, 777, 1175, 1393
Tru9I	TTAA	4 cut(s)	138, 777, 1175, 1393
TscAI	CASTG	4 cut(s)	100, 697, 749, 1219
TseFI	GTSAC	3 cut(s)	455, 883, 1265
TseI	GCWGC	3 cut(s)	718, 721, 1217
Tsp45I	GTSAC	3 cut(s)	455, 883, 1265
TspDTI	ATGAA	4 cut(s)	765, 1019, 1097, 1146
TspGWI	ACGGA	1 cut(s)	1245
TspRI	CASTG	4 cut(s)	100, 697, 749, 1219
VpaK11BI	GGWCC	2 cut(s)	1011, 1195
XapI	RAATTY	2 cut(s)	841, 1136
XceI	RCATGY	1 cut(s)	1266
XhoI	CTCGAG	1 cut(s)	124
XspI	CTAG	3 cut(s)	563, 660, 1125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.