Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004986

Physical Location & Seq

Genomic Coordinates Forward (+)

18316873 .. 18322302

Gene Structure 5430 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1076500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1689 bp

ATGGAGAAAATCATAGAGGACTTCGAAGCAATGACAAAGGATGCTGAGAGGGTTCAGAGAAAAACCCTGAAGAAGATTTTGGAAGAAAACGGATCCGTGGAGTATTTGCAGGGTTTCGGACTTAACGGAAGAACTGATCCAGAAAGTTTCAAGGCTTGTGTTCCGCTTGTTACTCACCAGGATGTGGACCCTTATATTCAGAGAATTGCAGATGGTGATTCCTCTCCCATCCTCACTGGGAAACCAGTCCCAACCTTCTCATTGAGCTCTGGTACTACTCAGGGAAGGCCAAAGTCCGTGCCTTTCAACGACGAATTGATGGAAACTACCATGCAGATATTTCAAACTTCTTTCGCGTTTAGAAACAGGGAGTACCCCATTGCGAATGGAAAGGCTTTGCAGTTTATCTATGGCAGCAAGCACCTGAAAACGCGAGGCGGTTTGGCAACCGGAACTGCCACCACCAACGTTTACCGCAATCAGAAGTTCAAAAACACAATGAAATCAGTGCAGTCTCAATGCTGCAGCCCAGACGAAGTGATATTCGGCCCCGATTTCCAACAATCGTTGTATTGCCATCTCTTGTGTGGCCTAATATTCAACGATGAAGTTCAGATGATATCCTCTACATTTGCTCATAGCATTGTCCTTGCATTCGGAACCTTCGAGCTGCTGTGGGAAGAACTATGTGCTAACATTCGAGATGGGGTACTCAGCGACCGAATCACAGTGCCATCAATCCGATCAGCTGTGTCGAAACTGCTCACCCCGAACCCTGAATTGGCTGATTTGATTCATGGAAAATGTTCAGGATTGAGTAACTGGTATGGATTGATACCAGCACTATTTCCCAATGCCAAGTACATTTATGGGATCATGACAGGGTCCATGGAGCCTTATTTGAAGAAACTGAGGCACTATGCGGGGGAGTTGCCATTGGTGAGTGCAGATTATGGCGCTTCTGAAGGGTGGATTGGGGCAAATGTCAACCCTACATTGCCCGCTGAGTTGTCTACTTTTGCTGTGCTCCCAAATATTGGGTACTTTGAGTTCATCCCTATGAAGAACAACCGTGGTGAGCAGCCAGTTGGACTCACTGAAGTCAAGGTTGGTGAAGAGTATGAGGTGGTCCTCACTAATGTTGCAGGTTTGTACCGTTACAGACTAGGAGATGTAGTGAAGGTTGTGGGGTTCTACAACTCAACTCCGGAGCTCAAGTTCGTGTGCCGGAGCAATCTTTTGCTTACTATTAACATTGACAAGAACACCGAGAAAGATTTGCAACTATCCGTTGAAGAAGCTGCAAAGATTTTAGCTGCAGAAAAGTTGGAAGTTGTTGATTTCTCAAGCCATGTGGACTTGTCCACGGATCCAGGTCACTATGTCATATTTCTGGAACTCAATGGTGAACCAAGCCATGAGGTTCTTCGTGAGTGCTGCAACTGTTTGGACAAATCTTTTGTTGATCCGGGATACATGAGTTCGCGCAAGGTCAACACCATAGGCCCGCTCGAGCTTCGAATTCTTCGTAAGGGAACCTTCCAAGAGATTCTAAACTATTACATAGCTCAAGGAACTGTTGTTAACCAGTTCAAAACACCAAGATGTGTAGGGCCTAACAACACCAAGGTTTTGCAACTCTTGTGTGGCAACGTGGTTAGGAGCTTCTTTAGTACTGCCTATAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

GH3

Protein Analysis

563

Amino Acids

62.59

Weight (kDa)

5.84

Isoelectric Point (pI)

33.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH3	PF03321	5 - 313	9.4e-113	GH3 family N-terminal domain
GH3_M	PF23571	341 - 410	8.4e-29	GH3 family central domain
GH3_C	PF23572	425 - 538	2.3e-32	GH3 family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46370 AT2G46370 AT2G46370 AT2G46370
fragaria_vesca	FvH4_7g16040 FvH4_7g16040 FvH4_7g16040 FvH4_7g16040 FvH4_7g16040 FvH4_7g16040
malus_domestica	MD01G1076500.v1.1 MD07G1145900.v1.1
prunus_persica	Prupe.2G184100_v2.0.a1 Prupe.2G184100_v2.0.a1 Prupe.2G184100_v2.0.a1 Prupe.2G184100_v2.0.a1
pyrus_communis	pycom01g10570 pycom07g14550 pycom07g14560
rosa_chinensis	RchiOBHm_Chr1g0358981
rosa_laevigata	RLG00000027911
rosa_multiflora	Rmu_sc0004044.1_g000003
rosa_roxburghii	Rroxscaffold_4G00297490
rosa_rugosa	Rorug01G0266700 Rorug01G0266800.1
rosa_samantha	Rh1AG280100 Rh1BG247100 Rh1CG264500 Rh1DG275400
rosa_wichuraiana	Rw1G024900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1136
AccB7I	CCANNNNNTGG	2 cut(s)	184, 1037
AccBSI	CCGCTC	1 cut(s)	1510
AccI	GTMKAC	1 cut(s)	1013
AccII	CGCG	3 cut(s)	356, 433, 1486
AccIII	TCCGGA	1 cut(s)	1207
AciI	CCGC	6 cut(s)	164, 438, 475, 923, 1002, 1508
AclI	AACGTT	1 cut(s)	468
AclWI	GGATC	7 cut(s)	87, 100, 131, 881, 1364, 1377, 1460
AcsI	RAATTY	1 cut(s)	1521
AcuI	CTGAAG	3 cut(s)	89, 984, 1119
AfaI	GTAC	7 cut(s)	274, 374, 711, 863, 1043, 1154, 1675
AfiI	CCNNNNNNNGG	3 cut(s)	184, 781, 1037
AgsI	TTSAA	8 cut(s)	151, 307, 344, 490, 601, 904, 1295, 1594
AjnI	CCWGG	2 cut(s)	177, 1372
AjuI	GAANNNNNNNTTGG	8 cut(s)	62, 94, 764, 796, 957, 989, 1092, 1124
AloI	GAACNNNNNNTCC	2 cut(s)	1202, 1234
AluBI	AGCT	9 cut(s)	267, 670, 749, 1213, 1301, 1316, 1516, 1568, 1665
AluI	AGCT	9 cut(s)	267, 670, 749, 1213, 1301, 1316, 1516, 1568, 1665
Alw21I	GWGCWC	3 cut(s)	269, 1029, 1215
Alw26I	GTCTC	1 cut(s)	519
AlwI	GGATC	7 cut(s)	87, 100, 131, 881, 1364, 1377, 1460
Ama87I	CYCGRG	1 cut(s)	1511
Aor13HI	TCCGGA	1 cut(s)	1207
AoxI	GGCC	5 cut(s)	287, 547, 589, 1504, 1613
ApeKI	GCWGC	8 cut(s)	414, 522, 525, 670, 1081, 1301, 1316, 1437
ApoI	RAATTY	1 cut(s)	1521
ArsI	GACNNNNNNTTYG	2 cut(s)	1442, 1474
Asp700I	GAANNNNTTC	1 cut(s)	805
AspLEI	GCGC	2 cut(s)	959, 1488
AspS9I	GGNCC	6 cut(s)	187, 548, 885, 1129, 1505, 1613
AsuC2I	CCSGG	1 cut(s)	1470
AsuHPI	GGTGA	7 cut(s)	167, 227, 757, 952, 1088, 1124, 1418
AsuII	TTCGAA	2 cut(s)	24, 1519
AvaI	CYCGRG	1 cut(s)	1511
AvaII	GGWCC	3 cut(s)	187, 885, 1129
BaeI	ACNNNNGTAYC	2 cut(s)	1465, 1498
BamHI	GGATCC	2 cut(s)	92, 1369
BanII	GRGCYC	2 cut(s)	269, 1215
Bbv12I	GWGCWC	3 cut(s)	269, 1029, 1215
BbvI	GCAGC	8 cut(s)	426, 509, 537, 657, 1093, 1288, 1303, 1424
BccI	CCATC	6 cut(s)	206, 236, 313, 585, 698, 742
BciT130I	CCWGG	2 cut(s)	179, 1374
BciVI	GTATCC	1 cut(s)	1466
BcnI	CCSGG	1 cut(s)	1470
BcoDI	GTCTC	1 cut(s)	519
BfaI	CTAG	1 cut(s)	1166
BfmI	CTRYAG	2 cut(s)	523, 1317
BfoI	RGCGCY	1 cut(s)	960
BfuAI	ACCTGC	1 cut(s)	1136
BfuI	GTATCC	1 cut(s)	1466
BisI	GCNGC	8 cut(s)	415, 523, 526, 671, 1082, 1302, 1317, 1438
BlsI	GCNGC	8 cut(s)	416, 524, 527, 672, 1083, 1303, 1318, 1439
BmcAI	AGTACT	1 cut(s)	1675
Bme1390I	CCNGG	3 cut(s)	179, 1374, 1470
Bme18I	GGWCC	3 cut(s)	187, 885, 1129
BmeT110I	CYCGRG	1 cut(s)	1511
BmgT120I	GGNCC	6 cut(s)	187, 548, 885, 1129, 1505, 1613
BmiI	GGNNCC	8 cut(s)	94, 189, 550, 661, 886, 894, 1371, 1537
BmrFI	CCNGG	3 cut(s)	179, 1374, 1470
BmrI	ACTGGG	1 cut(s)	246
BmsI	GCATC	1 cut(s)	31
BmuI	ACTGGG	1 cut(s)	246
Bpu14I	TTCGAA	2 cut(s)	24, 1519
BpuEI	CTTGAG	3 cut(s)	1199, 1330, 1554
BpuMI	CCSGG	1 cut(s)	1470
BsaJI	CCNNGG	5 cut(s)	96, 888, 1072, 1365, 1626
BsaWI	WCCGGW	2 cut(s)	449, 1207
BsaXI	ACNNNNNCTCC	4 cut(s)	1161, 1191, 1202, 1232
Bsc4I	CCNNNNNNNGG	3 cut(s)	184, 781, 1037
Bse1I	ACTGG	5 cut(s)	241, 245, 827, 1085, 1588
Bse3DI	GCAATG	3 cut(s)	36, 378, 995
BseAI	TCCGGA	1 cut(s)	1207
BseBI	CCWGG	2 cut(s)	179, 1374
BseDI	CCNNGG	5 cut(s)	96, 888, 1072, 1365, 1626
BseGI	GGATG	4 cut(s)	46, 187, 228, 1053
BseLI	CCNNNNNNNGG	3 cut(s)	184, 781, 1037
BseMI	GCAATG	3 cut(s)	36, 378, 995
BseMII	CTCAG	5 cut(s)	36, 293, 727, 902, 996
BseNI	ACTGG	5 cut(s)	241, 245, 827, 1085, 1588
BseXI	GCAGC	8 cut(s)	426, 509, 537, 657, 1093, 1288, 1303, 1424
BsgI	GTGCAG	2 cut(s)	530, 966
Bsh1236I	CGCG	3 cut(s)	356, 433, 1486
Bsh1285I	CGRYCG	1 cut(s)	721
BshFI	GGCC	5 cut(s)	289, 549, 591, 1506, 1615
BsiEI	CGRYCG	1 cut(s)	721
BsiHKAI	GWGCWC	3 cut(s)	269, 1029, 1215
BsiHKCI	CYCGRG	1 cut(s)	1511
BsiSI	CCGG	4 cut(s)	450, 1208, 1228, 1469
BslFI	GGGAC	1 cut(s)	233
BslI	CCNNNNNNNGG	3 cut(s)	184, 781, 1037
BsmAI	GTCTC	1 cut(s)	519
BsmFI	GGGAC	1 cut(s)	233
BsmI	GAATGC	1 cut(s)	653
BsnI	GGCC	5 cut(s)	289, 549, 591, 1506, 1615
BsoBI	CYCGRG	1 cut(s)	1511
Bsp119I	TTCGAA	2 cut(s)	24, 1519
Bsp1286I	GDGCHC	3 cut(s)	269, 1029, 1215
Bsp13I	TCCGGA	1 cut(s)	1207
Bsp143I	GATC	6 cut(s)	92, 136, 743, 873, 1369, 1465
Bsp19I	CCATGG	1 cut(s)	888
BspACI	CCGC	6 cut(s)	164, 438, 475, 923, 1002, 1508
BspANI	GGCC	5 cut(s)	289, 549, 591, 1506, 1615
BspCNI	CTCAG	5 cut(s)	37, 292, 726, 903, 997
BspEI	TCCGGA	1 cut(s)	1207
BspFNI	CGCG	3 cut(s)	356, 433, 1486
BspHI	TCATGA	1 cut(s)	876
BspLI	GGNNCC	8 cut(s)	94, 189, 550, 661, 886, 894, 1371, 1537
BspMAI	CTGCAG	2 cut(s)	527, 1321
BspMI	ACCTGC	1 cut(s)	1136
BspPI	GGATC	7 cut(s)	87, 100, 131, 881, 1364, 1377, 1460
BspT104I	TTCGAA	2 cut(s)	24, 1519
BsrBI	CCGCTC	1 cut(s)	1510
BsrDI	GCAATG	3 cut(s)	36, 378, 995
BsrI	ACTGG	5 cut(s)	241, 245, 827, 1085, 1588
BssECI	CCNNGG	5 cut(s)	96, 888, 1072, 1365, 1626
BssMI	GATC	6 cut(s)	92, 136, 743, 873, 1369, 1465
BssT1I	CCWWGG	2 cut(s)	888, 1626
Bst2UI	CCWGG	2 cut(s)	179, 1374
Bst4CI	ACNGT	5 cut(s)	730, 1073, 1157, 1445, 1579
Bst6I	CTCTTC	1 cut(s)	1110
BstBI	TTCGAA	2 cut(s)	24, 1519
BstC8I	GCNNGC	3 cut(s)	419, 1002, 1508
BstDEI	CTNAG	5 cut(s)	45, 279, 713, 911, 1005
BstDSI	CCRYGG	4 cut(s)	96, 888, 1072, 1365
BstF5I	GGATG	4 cut(s)	46, 187, 228, 1053
BstFNI	CGCG	3 cut(s)	356, 433, 1486
BstH2I	RGCGCY	1 cut(s)	960
BstHHI	GCGC	2 cut(s)	959, 1488
BstKTI	GATC	6 cut(s)	95, 139, 746, 876, 1372, 1468
BstMAI	GTCTC	1 cut(s)	519
BstMBI	GATC	6 cut(s)	92, 136, 743, 873, 1369, 1465
BstMCI	CGRYCG	1 cut(s)	721
BstNI	CCWGG	2 cut(s)	179, 1374
BstSCI	CCNGG	3 cut(s)	177, 1372, 1468
BstSFI	CTRYAG	2 cut(s)	523, 1317
BstUI	CGCG	3 cut(s)	356, 433, 1486
BstV1I	GCAGC	8 cut(s)	426, 509, 537, 657, 1093, 1288, 1303, 1424
BstX2I	RGATCY	2 cut(s)	92, 1369
BstYI	RGATCY	2 cut(s)	92, 1369
BsuI	GTATCC	1 cut(s)	1466
BsuRI	GGCC	5 cut(s)	289, 549, 591, 1506, 1615
BtgI	CCRYGG	4 cut(s)	96, 888, 1072, 1365
BtsCI	GGATG	4 cut(s)	46, 187, 228, 1053
BtsIMutI	CAGTG	4 cut(s)	234, 513, 735, 1095
BveI	ACCTGC	1 cut(s)	1136
Cac8I	GCNNGC	3 cut(s)	419, 1002, 1508
CciI	TCATGA	1 cut(s)	876
CfoI	GCGC	2 cut(s)	959, 1488
Cfr13I	GGNCC	6 cut(s)	187, 548, 885, 1129, 1505, 1613
Csp6I	GTAC	7 cut(s)	273, 373, 710, 862, 1042, 1153, 1674
CspCI	CAANNNNNGTGG	2 cut(s)	1335, 1370
CviAII	CATG	7 cut(s)	331, 797, 877, 889, 1352, 1418, 1477
CviQI	GTAC	7 cut(s)	273, 373, 710, 862, 1042, 1153, 1674
DdeI	CTNAG	5 cut(s)	45, 279, 713, 911, 1005
DpnI	GATC	6 cut(s)	94, 138, 745, 875, 1371, 1467
DpnII	GATC	6 cut(s)	92, 136, 743, 873, 1369, 1465
Eam1104I	CTCTTC	1 cut(s)	1110
EarI	CTCTTC	1 cut(s)	1110
Ecl136II	GAGCTC	2 cut(s)	267, 1213
Eco130I	CCWWGG	2 cut(s)	888, 1626
Eco24I	GRGCYC	2 cut(s)	269, 1215
Eco32I	GATATC	1 cut(s)	621
Eco47I	GGWCC	3 cut(s)	187, 885, 1129
Eco53kI	GAGCTC	2 cut(s)	267, 1213
Eco57I	CTGAAG	3 cut(s)	89, 984, 1119
Eco88I	CYCGRG	1 cut(s)	1511
EcoICRI	GAGCTC	2 cut(s)	267, 1213
EcoO109I	RGGNCCY	1 cut(s)	1613
EcoRI	GAATTC	1 cut(s)	1521
EcoRII	CCWGG	2 cut(s)	177, 1372
EcoRV	GATATC	1 cut(s)	621
EcoT14I	CCWWGG	2 cut(s)	888, 1626
EcoT38I	GRGCYC	2 cut(s)	269, 1215
ErhI	CCWWGG	2 cut(s)	888, 1626
FaeI	CATG	7 cut(s)	334, 800, 880, 892, 1355, 1421, 1480
FalI	AAGNNNNNCTT	2 cut(s)	1523, 1555
FaqI	GGGAC	1 cut(s)	233
FatI	CATG	7 cut(s)	330, 796, 876, 888, 1351, 1417, 1476
FauI	CCCGC	3 cut(s)	916, 1009, 1515
FblI	GTMKAC	1 cut(s)	1013
Fnu4HI	GCNGC	8 cut(s)	415, 523, 526, 671, 1082, 1302, 1317, 1438
FokI	GGATG	4 cut(s)	53, 194, 215, 1040
FriOI	GRGCYC	2 cut(s)	269, 1215
Fsp4HI	GCNGC	8 cut(s)	415, 523, 526, 671, 1082, 1302, 1317, 1438
FspBI	CTAG	1 cut(s)	1166
GlaI	GCGC	2 cut(s)	958, 1487
GluI	GCNGC	8 cut(s)	415, 523, 526, 671, 1082, 1302, 1317, 1438
HaeII	RGCGCY	1 cut(s)	960
HaeIII	GGCC	5 cut(s)	289, 549, 591, 1506, 1615
HapII	CCGG	4 cut(s)	450, 1208, 1228, 1469
HhaI	GCGC	2 cut(s)	959, 1488
Hin1II	CATG	7 cut(s)	334, 800, 880, 892, 1355, 1421, 1480
Hin6I	GCGC	2 cut(s)	957, 1486
HinP1I	GCGC	2 cut(s)	957, 1486
HincII	GTYRAC	3 cut(s)	988, 1495, 1585
HindII	GTYRAC	3 cut(s)	988, 1495, 1585
HinfI	GANTC	5 cut(s)	218, 723, 793, 1092, 1549
HpaI	GTTAAC	1 cut(s)	1585
HpaII	CCGG	4 cut(s)	450, 1208, 1228, 1469
HphI	GGTGA	7 cut(s)	167, 227, 757, 952, 1088, 1124, 1418
Hpy166II	GTNNAC	9 cut(s)	187, 472, 988, 1014, 1357, 1365, 1409, 1495, 1585
Hpy188I	TCNGA	8 cut(s)	57, 119, 201, 483, 615, 659, 743, 964
Hpy188III	TCNNGA	7 cut(s)	140, 701, 810, 877, 1208, 1394, 1430
Hpy8I	GTNNAC	9 cut(s)	187, 472, 988, 1014, 1357, 1365, 1409, 1495, 1585
Hpy99I	CGWCG	1 cut(s)	314
HpyAV	CCTTC	6 cut(s)	265, 279, 673, 959, 1174, 1549
HpyCH4III	ACNGT	5 cut(s)	730, 1073, 1157, 1445, 1579
HpyCH4IV	ACGT	2 cut(s)	468, 1653
HpyF3I	CTNAG	5 cut(s)	45, 279, 713, 911, 1005
HpySE526I	ACGT	2 cut(s)	468, 1653
Hsp92II	CATG	7 cut(s)	334, 800, 880, 892, 1355, 1421, 1480
HspAI	GCGC	2 cut(s)	957, 1486
Kpn2I	TCCGGA	1 cut(s)	1207
KspAI	GTTAAC	1 cut(s)	1585
Kzo9I	GATC	6 cut(s)	92, 136, 743, 873, 1369, 1465
LmnI	GCTCC	5 cut(s)	892, 1032, 1210, 1230, 1662
Lsp1109I	GCAGC	8 cut(s)	426, 509, 537, 657, 1093, 1288, 1303, 1424
LweI	GCATC	1 cut(s)	31
MaeI	CTAG	1 cut(s)	1166
MaeII	ACGT	2 cut(s)	468, 1653
MaeIII	GTNAC	4 cut(s)	169, 818, 1157, 1376
MalI	GATC	6 cut(s)	94, 138, 745, 875, 1371, 1467
MbiI	CCGCTC	1 cut(s)	1510
MboI	GATC	6 cut(s)	92, 136, 743, 873, 1369, 1465
MflI	RGATCY	2 cut(s)	92, 1369
MhlI	GDGCHC	3 cut(s)	269, 1029, 1215
MluCI	AATT	4 cut(s)	204, 314, 779, 1521
MlyI	GAGTC	1 cut(s)	1086
MmeI	TCCRAC	3 cut(s)	583, 1069, 1308
MroI	TCCGGA	1 cut(s)	1207
MroXI	GAANNNNTTC	1 cut(s)	805
MseI	TTAA	3 cut(s)	123, 1251, 1584
MslI	CAYNNNNRTG	3 cut(s)	180, 1058, 1603
MspA1I	CMGCKG	2 cut(s)	749, 1004
MspI	CCGG	4 cut(s)	450, 1208, 1228, 1469
MspR9I	CCNGG	3 cut(s)	179, 1374, 1470
Mva1269I	GAATGC	1 cut(s)	653
MvaI	CCWGG	2 cut(s)	179, 1374
MvnI	CGCG	3 cut(s)	356, 433, 1486
NciI	CCSGG	1 cut(s)	1470
NcoI	CCATGG	1 cut(s)	888
NdeII	GATC	6 cut(s)	92, 136, 743, 873, 1369, 1465
NlaIII	CATG	7 cut(s)	334, 800, 880, 892, 1355, 1421, 1480
NlaIV	GGNNCC	8 cut(s)	94, 189, 550, 661, 886, 894, 1371, 1537
NmuCI	GTSAC	1 cut(s)	1376
NspV	TTCGAA	2 cut(s)	24, 1519
PaeR7I	CTCGAG	1 cut(s)	1511
PagI	TCATGA	1 cut(s)	876
PcsI	WCGNNNNNNNCGW	2 cut(s)	663, 1525
PctI	GAATGC	1 cut(s)	653
PdmI	GAANNNNTTC	1 cut(s)	805
PfeI	GAWTC	4 cut(s)	218, 723, 793, 1549
PflFI	GACNNNGTC	1 cut(s)	883
PflMI	CCANNNNNTGG	2 cut(s)	184, 1037
PfoI	TCCNGGA	1 cut(s)	1468
PkrI	GCNGC	8 cut(s)	416, 524, 527, 672, 1083, 1303, 1318, 1439
PleI	GAGTC	1 cut(s)	1086
PpsI	GAGTC	1 cut(s)	1086
Psp124BI	GAGCTC	2 cut(s)	269, 1215
Psp1406I	AACGTT	1 cut(s)	468
Psp6I	CCWGG	2 cut(s)	177, 1372
PspGI	CCWGG	2 cut(s)	177, 1372
PspN4I	GGNNCC	8 cut(s)	94, 189, 550, 661, 886, 894, 1371, 1537
PspPI	GGNCC	6 cut(s)	187, 548, 885, 1129, 1505, 1613
PspXI	VCTCGAGB	1 cut(s)	1511
PsrI	GAACNNNNNNTAC	2 cut(s)	1034, 1066
PstI	CTGCAG	2 cut(s)	527, 1321
PsuI	RGATCY	2 cut(s)	92, 1369
PsyI	GACNNNGTC	1 cut(s)	883
PvuII	CAGCTG	1 cut(s)	749
RsaI	GTAC	7 cut(s)	274, 374, 711, 863, 1043, 1154, 1675
RsaNI	GTAC	7 cut(s)	273, 373, 710, 862, 1042, 1153, 1674
RseI	CAYNNNNRTG	3 cut(s)	180, 1058, 1603
SacI	GAGCTC	2 cut(s)	269, 1215
SaqAI	TTAA	3 cut(s)	123, 1251, 1584
SatI	GCNGC	8 cut(s)	415, 523, 526, 671, 1082, 1302, 1317, 1438
Sau3AI	GATC	6 cut(s)	92, 136, 743, 873, 1369, 1465
Sau96I	GGNCC	6 cut(s)	187, 548, 885, 1129, 1505, 1613
ScaI	AGTACT	1 cut(s)	1675
SchI	GAGTC	1 cut(s)	1086
ScrFI	CCNGG	3 cut(s)	179, 1374, 1470
SduI	GDGCHC	3 cut(s)	269, 1029, 1215
SfaNI	GCATC	1 cut(s)	31
SfcI	CTRYAG	2 cut(s)	523, 1317
Sfr274I	CTCGAG	1 cut(s)	1511
SfuI	TTCGAA	2 cut(s)	24, 1519
SinI	GGWCC	3 cut(s)	187, 885, 1129
SlaI	CTCGAG	1 cut(s)	1511
SmiMI	CAYNNNNRTG	3 cut(s)	180, 1058, 1603
SmlI	CTYRAG	4 cut(s)	1214, 1345, 1511, 1569
SmoI	CTYRAG	4 cut(s)	1214, 1345, 1511, 1569
Sse9I	AATT	4 cut(s)	204, 314, 779, 1521
SsiI	CCGC	6 cut(s)	164, 438, 475, 923, 1002, 1508
SspI	AATATT	2 cut(s)	597, 1036
SspMI	CTAG	1 cut(s)	1166
SstI	GAGCTC	2 cut(s)	269, 1215
StyD4I	CCNGG	3 cut(s)	177, 1372, 1468
StyI	CCWWGG	2 cut(s)	888, 1626
TaaI	ACNGT	5 cut(s)	730, 1073, 1157, 1445, 1579
TaiI	ACGT	2 cut(s)	471, 1656
TaqI	TCGA	6 cut(s)	24, 666, 700, 755, 1512, 1519
TaqII	GACCGA	1 cut(s)	735
TasI	AATT	4 cut(s)	204, 314, 779, 1521
TatI	WGTACW	2 cut(s)	861, 1673
TfiI	GAWTC	4 cut(s)	218, 723, 793, 1549
Tru1I	TTAA	3 cut(s)	123, 1251, 1584
Tru9I	TTAA	3 cut(s)	123, 1251, 1584
TscAI	CASTG	4 cut(s)	241, 513, 735, 1102
TseFI	GTSAC	1 cut(s)	1376
TseI	GCWGC	8 cut(s)	414, 522, 525, 670, 1081, 1301, 1316, 1437
Tsp45I	GTSAC	1 cut(s)	1376
TspDTI	ATGAA	5 cut(s)	515, 621, 785, 1042, 1076
TspGWI	ACGGA	6 cut(s)	85, 105, 141, 286, 1279, 1382
TspRI	CASTG	4 cut(s)	241, 513, 735, 1102
Tth111I	GACNNNGTC	1 cut(s)	883
Van91I	CCANNNNNTGG	2 cut(s)	184, 1037
VpaK11BI	GGWCC	3 cut(s)	187, 885, 1129
XapI	RAATTY	1 cut(s)	1521
XcmI	CCANNNNNNNNNTGG	1 cut(s)	584
XhoI	CTCGAG	1 cut(s)	1511
XmiI	GTMKAC	1 cut(s)	1013
XmnI	GAANNNNTTC	1 cut(s)	805
XspI	CTAG	1 cut(s)	1166
ZrmI	AGTACT	1 cut(s)	1675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.