MD01G1086700.v1.1

P21-Rho-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014369
Physical Location & Seq
Forward (+)
19534709 .. 19538856
4148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1086700.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGGCTCGGCTCTTCCGATCCAAATCTTGCCATCTTGTTGGACTCACTGAGTTCAGCATTCCCCCTCCTAGTCCATTCTCCTACCACCACAACACCAGCAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGATGATGAAGGGCTGGAATATGAGGAGTTGGATTTTGAAGACGAGGAGGAGGCTGTAGGAAATCCCAGGTCTACCCCATTTCTGAGTCCCAAGATTAGAGCCAGTGGGAACAACAAAGGTCGAGACTTCCACGGGCATAACGGTCGACAACACCAGCACCACCAGTCCGCCATACTCGACATTTTGGTGGCGGCTCTGAGGAAGTCTCTGGTAACTTGCAGTGTGGAGAGGGATGACGTGGCGTCCTCTTTCGACATCAGTTCTCCGAGCGAGGTGAGACATGTCTCGCATGTCACTTTTGATAGGTTTAATGGGTTTCTGGGTTTGCCCACTGAGCTCGAGCCCGAGGTCCCCAGGAAGGTGCCAAGTGCCAGTGTAAGTGTGTTTGGGGTCTCTGCCAAGTCGATGCAGTGTTCTTATGATAATAATGGGAACAGTGTGCCAACTATACTTCTTATGATGCAAGAACGATTGTATTCAGGAGGAGGCCTTAAAGCAGAAGGAATATTCAGAATTAATGCTGAAAATGGTCAAGAAGAGCGTCTTCGCGACCAGTTGAACAAAGGCATTGTGCCACATGGAATTGATGTTCATTGTTTAGCAGGTTTGATAAAGGCATGGTTTAGAGAGCTTCCTACAAGGGTACTTGATTCGCTCACACCAGAACAGGTGATGAGCTGCAACACAGAAGATGACTGCACAGAACTTGTGAAGACACTTCCTCCAACAGAAGCTTCACTGCTAGATTGGGCCATCAATTTGATGGCAGATGTTGCGCAGAACGAACAGCACAACAAGATGAATGCCCGCAACATTGCTATGGTTTTTGCACCTAACATGACCGAGATGGCAGATCCCTTGACTGCACTAATACACGCGGTGCAAGTTATGAATTTCCTCAAGACATTGATTTTAAAGACCCTGCGCACAAGAGAGGAATCAGCTGCGGAGGCTACATTGCACGCATCATGCAAAAAATCTCTTAACCACAACAGACAAACCATGTATTCGATTATGGGTGAATCTTTTGTTCATGATAGCTCATTCAGCACCGCCACTTTGGACAGCCCAAAAGCTGACATCAATGAGAAACTTTGGTGCTCTCCGGTAATGACTGATGGGGAAGATGAACTCGAATCCACTTCGCACAGCAGCGCATCTACTAAGTGTGAACTCGAGTGTTCAGAAAACGGATGTAGAAGTGGATATGAAGCTGGAGACTGGTTAAGTTGGAGGAAAGGAGTGCGGAAGCTATCCAGCCACCTGGTGTTTCAGTTGAGTAAGCCGGCTAAGAAGAGTACGAAACTTGAGATTGTAAATACTAGGGGAGAAGGTGGAGAAGCATGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0008150 GO:0009505 GO:0009531 GO:0009664 GO:0009987 GO:0016020 GO:0016043 GO:0030312 GO:0044464 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

496

Amino Acids

55.08

Weight (kDa)

5.15

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 193 - 327 2.5e-23 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46710
fragaria_vesca FvH4_7g17360
malus_domestica MD01G1086700.v1.1 MD07G1156800.v1.1
prunus_persica Prupe.2G195100_v2.0.a1
pyrus_communis pycom01g11060 pycom07g15340
rosa_chinensis RchiOBHm_Chr1g0360801
rosa_laevigata RLG00000027785
rosa_multiflora Rmu_ssc0000022.1_g000034
rosa_roxburghii Rroxscaffold_4G00295800
rosa_rugosa Rorug01G0281600
rosa_samantha Rh1AG293600 Rh1BG258200 Rh1CG276100 Rh1DG288500
rosa_wichuraiana Rw1G025980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 912, 1061
Acc36I ACCTGC 1 cut(s) 728
AccB1I GGYRCC 1 cut(s) 496
AccI GTMKAC 2 cut(s) 206, 280
AccII CGCG 2 cut(s) 684, 1013
AciI CCGC 7 cut(s) 303, 326, 943, 1013, 1082, 1188, 1381
AclWI GGATC 2 cut(s) 12, 983
AcsI RAATTY 1 cut(s) 1027
AcyI GRCGYC 1 cut(s) 377
AdeI CACNNNGTG 2 cut(s) 1015, 1402
AfaI GTAC 2 cut(s) 780, 1435
AfiI CCNNNNNNNGG 1 cut(s) 493
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 2 cut(s) 173, 694
AhdI GACNNNNNGTC 1 cut(s) 376
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 3 cut(s) 200, 488, 1398
AjuI GAANNNNNNNTTGG 2 cut(s) 853, 885
AloI GAACNNNNNNTCC 2 cut(s) 708, 740
AluBI AGCT 9 cut(s) 472, 766, 813, 869, 1079, 1176, 1211, 1349, 1387
AluI AGCT 9 cut(s) 472, 766, 813, 869, 1079, 1176, 1211, 1349, 1387
Alw21I GWGCWC 2 cut(s) 474, 1238
Alw26I GTCTC 6 cut(s) 252, 345, 406, 424, 532, 1347
AlwI GGATC 2 cut(s) 12, 983
Ama87I CYCGRG 3 cut(s) 473, 479, 1310
AoxI GGCC 2 cut(s) 622, 885
ApeKI GCWGC 3 cut(s) 813, 1079, 1287
ApoI RAATTY 1 cut(s) 1027
AseI ATTAAT 1 cut(s) 651
AspLEI GCGC 3 cut(s) 913, 1062, 1292
AspS9I GGNCC 2 cut(s) 484, 885
AsuHPI GGTGA 3 cut(s) 421, 817, 1166
AvaI CYCGRG 3 cut(s) 473, 479, 1310
AvaII GGWCC 1 cut(s) 484
BanI GGYRCC 1 cut(s) 496
BanII GRGCYC 2 cut(s) 474, 480
BbsI GAAGAC 3 cut(s) 180, 671, 854
Bbv12I GWGCWC 2 cut(s) 474, 1238
BbvI GCAGC 3 cut(s) 800, 1066, 1299
BccI CCATC 5 cut(s) 39, 892, 896, 976, 1247
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 3 cut(s) 202, 490, 1400
BcoDI GTCTC 6 cut(s) 252, 345, 406, 424, 532, 1347
BfaI CTAG 3 cut(s) 69, 878, 1458
BfmI CTRYAG 1 cut(s) 189
BfuAI ACCTGC 1 cut(s) 728
BisI GCNGC 4 cut(s) 327, 814, 1080, 1288
BlsI GCNGC 4 cut(s) 328, 815, 1081, 1289
Bme1390I CCNGG 3 cut(s) 202, 490, 1400
Bme18I GGWCC 1 cut(s) 484
BmeRI GACNNNNNGTC 1 cut(s) 376
BmeT110I CYCGRG 3 cut(s) 473, 479, 1310
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 484, 885
BmiI GGNNCC 2 cut(s) 486, 498
BmrFI CCNGG 3 cut(s) 202, 490, 1400
BmsI GCATC 4 cut(s) 531, 585, 1109, 1301
BpiI GAAGAC 3 cut(s) 180, 671, 854
BplI GAGNNNNNCTC 2 cut(s) 325, 357
BpmI CTGGAG 1 cut(s) 1371
BpuEI CTTGAG 2 cut(s) 1019, 1463
BsaBI GATNNNNATC 1 cut(s) 22
BsaHI GRCGYC 1 cut(s) 377
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 4 cut(s) 200, 265, 480, 488
BsaWI WCCGGW 1 cut(s) 1240
BsaXI ACNNNNNCTCC 2 cut(s) 841, 871
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse118I RCCGGY 1 cut(s) 1420
Bse1I ACTGG 5 cut(s) 237, 298, 507, 688, 1361
Bse3DI GCAATG 2 cut(s) 948, 1091
Bse8I GATNNNNATC 1 cut(s) 22
BseBI CCWGG 3 cut(s) 202, 490, 1400
BseDI CCNNGG 4 cut(s) 200, 265, 480, 488
BseGI GGATG 2 cut(s) 373, 1334
BseJI GATNNNNATC 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMI GCAATG 2 cut(s) 948, 1091
BseMII CTCAG 4 cut(s) 39, 209, 323, 459
BseNI ACTGG 5 cut(s) 237, 298, 507, 688, 1361
BseRI GAGGAG 4 cut(s) 173, 194, 197, 633
BseXI GCAGC 3 cut(s) 800, 1066, 1299
BsgI GTGCAG 2 cut(s) 817, 984
Bsh1236I CGCG 2 cut(s) 684, 1013
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 2 cut(s) 624, 887
BshNI GGYRCC 1 cut(s) 496
BsiEI CGRYCG 1 cut(s) 280
BsiHKAI GWGCWC 2 cut(s) 474, 1238
BsiHKCI CYCGRG 3 cut(s) 473, 479, 1310
BsiSI CCGG 2 cut(s) 1241, 1421
BslFI GGGAC 2 cut(s) 207, 470
BslI CCNNNNNNNGG 1 cut(s) 493
BsmAI GTCTC 6 cut(s) 252, 345, 406, 424, 532, 1347
BsmFI GGGAC 2 cut(s) 207, 470
BsmI GAATGC 2 cut(s) 57, 943
BsnI GGCC 2 cut(s) 624, 887
Bso31I GGTCTC 1 cut(s) 532
BsoBI CYCGRG 3 cut(s) 473, 479, 1310
Bsp1286I GDGCHC 3 cut(s) 474, 480, 1238
Bsp143I GATC 2 cut(s) 17, 988
Bsp68I TCGCGA 1 cut(s) 684
BspACI CCGC 7 cut(s) 303, 326, 943, 1013, 1082, 1188, 1381
BspANI GGCC 2 cut(s) 624, 887
BspCNI CTCAG 4 cut(s) 40, 210, 324, 460
BspFNI CGCG 2 cut(s) 684, 1013
BspHI TCATGA 1 cut(s) 1168
BspLI GGNNCC 2 cut(s) 486, 498
BspMI ACCTGC 1 cut(s) 728
BspPI GGATC 2 cut(s) 12, 983
BspQI GCTCTTC 2 cut(s) 17, 666
BspT107I GGYRCC 1 cut(s) 496
BspTNI GGTCTC 1 cut(s) 532
BsrDI GCAATG 2 cut(s) 948, 1091
BsrFI RCCGGY 1 cut(s) 1420
BsrI ACTGG 5 cut(s) 237, 298, 507, 688, 1361
BssAI RCCGGY 1 cut(s) 1420
BssECI CCNNGG 4 cut(s) 200, 265, 480, 488
BssMI GATC 2 cut(s) 17, 988
BssNI GRCGYC 1 cut(s) 377
Bst2UI CCWGG 3 cut(s) 202, 490, 1400
Bst4CI ACNGT 2 cut(s) 278, 572
Bst6I CTCTTC 3 cut(s) 17, 666, 1424
BstACI GRCGYC 1 cut(s) 377
BstAPI GCANNNNNTGC 1 cut(s) 908
BstC8I GCNNGC 3 cut(s) 943, 1098, 1422
BstDEI CTNAG 6 cut(s) 48, 218, 332, 468, 1299, 1425
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 2 cut(s) 373, 1334
BstFNI CGCG 2 cut(s) 684, 1013
BstHHI GCGC 3 cut(s) 913, 1062, 1292
BstKTI GATC 2 cut(s) 20, 991
BstMAI GTCTC 6 cut(s) 252, 345, 406, 424, 532, 1347
BstMBI GATC 2 cut(s) 17, 988
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 469, 908, 1085, 1182
BstNI CCWGG 3 cut(s) 202, 490, 1400
BstNSI RCATGY 2 cut(s) 419, 428
BstSCI CCNGG 3 cut(s) 200, 488, 1398
BstSFI CTRYAG 1 cut(s) 189
BstUI CGCG 2 cut(s) 684, 1013
BstV1I GCAGC 3 cut(s) 800, 1066, 1299
BstV2I GAAGAC 3 cut(s) 180, 671, 854
BstX2I RGATCY 1 cut(s) 988
BstXI CCANNNNNNTGG 2 cut(s) 38, 1399
BstYI RGATCY 1 cut(s) 988
BsuRI GGCC 2 cut(s) 624, 887
BtgI CCRYGG 1 cut(s) 265
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 2 cut(s) 373, 1334
BtsI GCAGTG 3 cut(s) 361, 551, 872
BtsIMutI CAGTG 8 cut(s) 45, 244, 361, 465, 514, 551, 577, 872
BtuMI TCGCGA 1 cut(s) 684
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 3 cut(s) 943, 1098, 1422
CciI TCATGA 1 cut(s) 1168
CfoI GCGC 3 cut(s) 913, 1062, 1292
Cfr10I RCCGGY 1 cut(s) 1420
Cfr13I GGNCC 2 cut(s) 484, 885
CseI GACGC 2 cut(s) 366, 665
CsiI ACCWGGT 1 cut(s) 1398
Csp6I GTAC 2 cut(s) 779, 1434
CviAII CATG 9 cut(s) 416, 425, 713, 753, 973, 1104, 1138, 1169, 1479
CviQI GTAC 2 cut(s) 779, 1434
DdeI CTNAG 6 cut(s) 48, 218, 332, 468, 1299, 1425
DpnI GATC 2 cut(s) 19, 990
DpnII GATC 2 cut(s) 17, 988
DraI TTTAAA 1 cut(s) 1050
DraIII CACNNNGTG 2 cut(s) 1015, 1402
DriI GACNNNNNGTC 1 cut(s) 376
Eam1104I CTCTTC 3 cut(s) 17, 666, 1424
Eam1105I GACNNNNNGTC 1 cut(s) 376
EarI CTCTTC 3 cut(s) 17, 666, 1424
EciI GGCGGA 1 cut(s) 292
Ecl136II GAGCTC 1 cut(s) 472
Eco147I AGGCCT 1 cut(s) 624
Eco24I GRGCYC 2 cut(s) 474, 480
Eco31I GGTCTC 1 cut(s) 532
Eco47I GGWCC 1 cut(s) 484
Eco53kI GAGCTC 1 cut(s) 472
Eco88I CYCGRG 3 cut(s) 473, 479, 1310
EcoICRI GAGCTC 1 cut(s) 472
EcoO109I RGGNCCY 1 cut(s) 484
EcoRII CCWGG 3 cut(s) 200, 488, 1398
EcoT38I GRGCYC 2 cut(s) 474, 480
FaeI CATG 9 cut(s) 419, 428, 716, 756, 976, 1107, 1141, 1172, 1482
FalI AAGNNNNNCTT 2 cut(s) 663, 695
FaqI GGGAC 2 cut(s) 207, 470
FatI CATG 9 cut(s) 415, 424, 712, 752, 972, 1103, 1137, 1168, 1478
FauI CCCGC 1 cut(s) 950
FblI GTMKAC 2 cut(s) 206, 280
Fnu4HI GCNGC 4 cut(s) 327, 814, 1080, 1288
FokI GGATG 2 cut(s) 380, 1341
FriOI GRGCYC 2 cut(s) 474, 480
Fsp4HI GCNGC 4 cut(s) 327, 814, 1080, 1288
FspBI CTAG 3 cut(s) 69, 878, 1458
FspI TGCGCA 2 cut(s) 912, 1061
GlaI GCGC 3 cut(s) 912, 1061, 1291
GluI GCNGC 4 cut(s) 327, 814, 1080, 1288
GsuI CTGGAG 1 cut(s) 1371
HaeIII GGCC 2 cut(s) 624, 887
HapII CCGG 2 cut(s) 1241, 1421
HgaI GACGC 2 cut(s) 366, 665
HhaI GCGC 3 cut(s) 913, 1062, 1292
Hin1I GRCGYC 1 cut(s) 377
Hin1II CATG 9 cut(s) 419, 428, 716, 756, 976, 1107, 1141, 1172, 1482
Hin6I GCGC 3 cut(s) 911, 1060, 1290
HinP1I GCGC 3 cut(s) 911, 1060, 1290
HincII GTYRAC 1 cut(s) 281
HindII GTYRAC 1 cut(s) 281
HindIII AAGCTT 1 cut(s) 867
HinfI GANTC 6 cut(s) 42, 220, 785, 1073, 1157, 1271
HpaII CCGG 2 cut(s) 1241, 1421
HphI GGTGA 3 cut(s) 421, 817, 1166
Hpy166II GTNNAC 3 cut(s) 207, 281, 1307
Hpy188I TCNGA 6 cut(s) 17, 219, 333, 402, 647, 1321
Hpy188III TCNNGA 6 cut(s) 257, 615, 668, 683, 1036, 1169
Hpy8I GTNNAC 3 cut(s) 207, 281, 1307
HpyAV CCTTC 4 cut(s) 137, 487, 629, 1460
HpyCH4III ACNGT 2 cut(s) 278, 572
HpyCH4IV ACGT 1 cut(s) 372
HpyF10VI GCNNNNNNNGC 4 cut(s) 469, 908, 1085, 1182
HpyF3I CTNAG 6 cut(s) 48, 218, 332, 468, 1299, 1425
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 377
Hsp92II CATG 9 cut(s) 419, 428, 716, 756, 976, 1107, 1141, 1172, 1482
HspAI GCGC 3 cut(s) 911, 1060, 1290
KroI GCCGGC 1 cut(s) 1420
KroNI GCCGGC 1 cut(s) 1422
Kzo9I GATC 2 cut(s) 17, 988
LguI GCTCTTC 2 cut(s) 17, 666
Lsp1109I GCAGC 3 cut(s) 800, 1066, 1299
LweI GCATC 4 cut(s) 531, 585, 1109, 1301
MabI ACCWGGT 1 cut(s) 1398
MaeI CTAG 3 cut(s) 69, 878, 1458
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 346, 427
MalI GATC 2 cut(s) 19, 990
MboI GATC 2 cut(s) 17, 988
MflI RGATCY 1 cut(s) 988
MhlI GDGCHC 3 cut(s) 474, 480, 1238
MluCI AATT 4 cut(s) 648, 717, 892, 1027
MlyI GAGTC 2 cut(s) 36, 229
MmeI TCCRAC 4 cut(s) 19, 144, 884, 1346
MroNI GCCGGC 1 cut(s) 1420
MseI TTAA 6 cut(s) 444, 627, 651, 1049, 1119, 1361
MslI CAYNNNNRTG 2 cut(s) 320, 953
MspA1I CMGCKG 1 cut(s) 1079
MspI CCGG 2 cut(s) 1241, 1421
MspR9I CCNGG 3 cut(s) 202, 490, 1400
Mva1269I GAATGC 2 cut(s) 57, 943
MvaI CCWGG 3 cut(s) 202, 490, 1400
MvnI CGCG 2 cut(s) 684, 1013
MwoI GCNNNNNNNGC 4 cut(s) 469, 908, 1085, 1182
NaeI GCCGGC 1 cut(s) 1422
NdeII GATC 2 cut(s) 17, 988
NgoMIV GCCGGC 1 cut(s) 1420
NlaIII CATG 9 cut(s) 419, 428, 716, 756, 976, 1107, 1141, 1172, 1482
NlaIV GGNNCC 2 cut(s) 486, 498
NmuCI GTSAC 1 cut(s) 427
NruI TCGCGA 1 cut(s) 684
NsbI TGCGCA 2 cut(s) 912, 1061
NspI RCATGY 2 cut(s) 419, 428
PaeR7I CTCGAG 2 cut(s) 473, 1310
PagI TCATGA 1 cut(s) 1168
PceI AGGCCT 1 cut(s) 624
PciI ACATGT 1 cut(s) 415
PciSI GCTCTTC 2 cut(s) 17, 666
PcsI WCGNNNNNNNCGW 1 cut(s) 13
PctI GAATGC 2 cut(s) 57, 943
PdiI GCCGGC 1 cut(s) 1422
PfeI GAWTC 4 cut(s) 785, 1073, 1157, 1271
PkrI GCNGC 4 cut(s) 328, 815, 1081, 1289
PleI GAGTC 2 cut(s) 36, 228
PpsI GAGTC 2 cut(s) 36, 228
PpuMI RGGWCCY 1 cut(s) 484
PscI ACATGT 1 cut(s) 415
PshBI ATTAAT 1 cut(s) 651
Psp124BI GAGCTC 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 3 cut(s) 200, 488, 1398
PspGI CCWGG 3 cut(s) 200, 488, 1398
PspN4I GGNNCC 2 cut(s) 486, 498
PspPI GGNCC 2 cut(s) 484, 885
PspPPI RGGWCCY 1 cut(s) 484
PspXI VCTCGAGB 2 cut(s) 473, 1310
PsuI RGATCY 1 cut(s) 988
PvuII CAGCTG 1 cut(s) 1079
RruI TCGCGA 1 cut(s) 684
RsaI GTAC 2 cut(s) 780, 1435
RsaNI GTAC 2 cut(s) 779, 1434
RseI CAYNNNNRTG 2 cut(s) 320, 953
SacI GAGCTC 1 cut(s) 474
SalI GTCGAC 1 cut(s) 279
SapI GCTCTTC 2 cut(s) 17, 666
SaqAI TTAA 6 cut(s) 444, 627, 651, 1049, 1119, 1361
SatI GCNGC 4 cut(s) 327, 814, 1080, 1288
Sau3AI GATC 2 cut(s) 17, 988
Sau96I GGNCC 2 cut(s) 484, 885
SchI GAGTC 2 cut(s) 36, 229
ScrFI CCNGG 3 cut(s) 202, 490, 1400
SduI GDGCHC 3 cut(s) 474, 480, 1238
SexAI ACCWGGT 1 cut(s) 1398
SfaNI GCATC 4 cut(s) 531, 585, 1109, 1301
SfcI CTRYAG 1 cut(s) 189
Sfr274I CTCGAG 2 cut(s) 473, 1310
SinI GGWCC 1 cut(s) 484
SlaI CTCGAG 2 cut(s) 473, 1310
SmiMI CAYNNNNRTG 2 cut(s) 320, 953
SmlI CTYRAG 4 cut(s) 473, 1034, 1310, 1442
SmoI CTYRAG 4 cut(s) 473, 1034, 1310, 1442
Sse9I AATT 4 cut(s) 648, 717, 892, 1027
SseBI AGGCCT 1 cut(s) 624
SsiI CCGC 7 cut(s) 303, 326, 943, 1013, 1082, 1188, 1381
SspI AATATT 1 cut(s) 642
SspMI CTAG 3 cut(s) 69, 878, 1458
SstI GAGCTC 1 cut(s) 474
StuI AGGCCT 1 cut(s) 624
StyD4I CCNGG 3 cut(s) 200, 488, 1398
TaaI ACNGT 2 cut(s) 278, 572
TaiI ACGT 1 cut(s) 375
TaqI TCGA 9 cut(s) 256, 280, 312, 387, 474, 539, 1145, 1269, 1311
TaqII GACCGA 1 cut(s) 992
TasI AATT 4 cut(s) 648, 717, 892, 1027
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 4 cut(s) 785, 1073, 1157, 1271
Tru1I TTAA 6 cut(s) 444, 627, 651, 1049, 1119, 1361
Tru9I TTAA 6 cut(s) 444, 627, 651, 1049, 1119, 1361
TscAI CASTG 8 cut(s) 52, 244, 361, 472, 514, 551, 577, 879
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 3 cut(s) 813, 1079, 1287
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 7 cut(s) 156, 716, 950, 1040, 1157, 1278, 1359
TspGWI ACGGA 1 cut(s) 1341
TspRI CASTG 8 cut(s) 52, 244, 361, 472, 514, 551, 577, 879
VpaK11BI GGWCC 1 cut(s) 484
VspI ATTAAT 1 cut(s) 651
XapI RAATTY 1 cut(s) 1027
XceI RCATGY 2 cut(s) 419, 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 895
XhoI CTCGAG 2 cut(s) 473, 1310
XmiI GTMKAC 2 cut(s) 206, 280
XspI CTAG 3 cut(s) 69, 878, 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.