Rmu_ssc0000022.1_g000034

P21-Rho-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000022.1
OG0014369
Physical Location & Seq
Forward (+)
178661 .. 182303
3643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000022.1_g000034.1.cds

Sequence Viewer

Length: 1449 bp
atggctcgtctctcccgatccaaatcttgcggactcgtcggactcaccgagttcaccgtcgctcctccgacccagttctgccaaaaccatgaagacgaaacagagagagaagaagacgaggaagacgaaaaagggtctgaatttgatgaagaagacgacgagtcgtgtgaggtgtcgaccccatttctcagtccgatgagcagagccaatggggctcataaaggtcgagactttaacagtagtgggccgcagaacaaccagtttgcgatcatggatattctggtggctgctctgaggaagtctctgattacttgcagtgtggagacggacgacgtggcgtcctcctttgatataagttcacctaccgaggtgaggcatgtttcgcatgtgacctttgataggtttaatgggtttctgggtttgcctactgagcttgagcctgaggttcccaggaaggcacccagtgctagtgctagtgtgtttggggtctctgctaagtcgatgcagtgttcttatgatgatagaggaaacagtgtgccaacaatacttcttatgatgcaaaagcgattgtactcaggaggaggccttaaagcagaaggaatattcagaataaatgctgaaaatagccaagaagagcgtgttcggaaccagttgaatagtggtgtagtgccacatggaattgatgttcattgcttagcaggtttaataaaggcatggtttagagagcttccgacacgggtgcttgattctcttacaccagaacaggtgatgcgctgcaacacagaagatgactgcacaggacttgtaaagtcacttcctgcaacggaagcatcactgctagattgggccctcaatttgatggcagatgttgcgcagaatgaacaacacaataagatgaatgctcgcaacattgccatggtttttgctccgaacatgacagagatggcagatcctttaacagcactgctgcatgcagtgcaagttatgaatttcctcaagacactaatcttaaagatcctgcgcgaaagagaggaatcagctgctgctgaggcttcgcagagctcatcttgctcaaaatctcccaaccacaactatgatcttccttattccattatgggacatgttcaggagaactcattgaggacaaccactgtggacatccctgaagcctgcattgacgagaaactttggtgctctcctgtaatgagtgatgaggaagaggaaatcgaatccgattccaatagtagcccatctagcaagcatgaacttgagtgttcggaaacagtttgtagatcaggtggatatgaagctggagactggttaagtttgaggaaaggagtgcggaagctgtgcaaccacccggtctttcagttgagtaagccagctaagaagactgcaaatgttgggattgtaaatactaggggaggggatggagaagcttgggcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0008150 GO:0009505 GO:0009531 GO:0009664 GO:0009987 GO:0016020 GO:0016043 GO:0030312 GO:0044464 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

482

Amino Acids

53.03

Weight (kDa)

4.85

Isoelectric Point (pI)

55.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46710
fragaria_vesca FvH4_7g17360
malus_domestica MD01G1086700.v1.1 MD07G1156800.v1.1
prunus_persica Prupe.2G195100_v2.0.a1
pyrus_communis pycom01g11060 pycom07g15340
rosa_chinensis RchiOBHm_Chr1g0360801
rosa_laevigata RLG00000027785
rosa_multiflora Rmu_ssc0000022.1_g000034
rosa_roxburghii Rroxscaffold_4G00295800
rosa_rugosa Rorug01G0281600
rosa_samantha Rh1AG293600 Rh1BG258200 Rh1CG276100 Rh1DG288500
rosa_wichuraiana Rw1G025980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 873
Acc36I ACCTGC 1 cut(s) 689
AccB1I GGYRCC 1 cut(s) 457
AccI GTMKAC 1 cut(s) 176
AccII CGCG 1 cut(s) 1023
AciI CCGC 3 cut(s) 30, 248, 1342
AclWI GGATC 3 cut(s) 12, 944, 1009
AcsI RAATTY 2 cut(s) 140, 988
AcuI CTGAAG 1 cut(s) 1185
AcyI GRCGYC 1 cut(s) 338
AdeI CACNNNGTG 1 cut(s) 464
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 2 cut(s) 372, 399
AflIII ACRYGT 1 cut(s) 1120
AgsI TTSAA 1 cut(s) 655
AhdI GACNNNNNGTC 2 cut(s) 160, 337
AjiI CACGTC 1 cut(s) 334
AjnI CCWGG 1 cut(s) 449
AloI GAACNNNNNNTCC 2 cut(s) 669, 701
AluBI AGCT 8 cut(s) 433, 727, 1040, 1062, 1310, 1348, 1385, 1439
AluI AGCT 8 cut(s) 433, 727, 1040, 1062, 1310, 1348, 1385, 1439
Alw21I GWGCWC 2 cut(s) 1064, 1196
Alw26I GTCTC 6 cut(s) 14, 222, 306, 317, 493, 1308
AlwI GGATC 3 cut(s) 12, 944, 1009
AlwNI CAGNNNCTG 1 cut(s) 1043
AoxI GGCC 3 cut(s) 245, 583, 846
ApaI GGGCCC 1 cut(s) 850
ApeKI GCWGC 5 cut(s) 287, 774, 967, 1040, 1043
ApoI RAATTY 2 cut(s) 140, 988
AspLEI GCGC 3 cut(s) 774, 874, 1023
AspS9I GGNCC 3 cut(s) 245, 846, 847
AsuC2I CCSGG 1 cut(s) 1361
AsuHPI GGTGA 5 cut(s) 37, 46, 351, 382, 778
AxyI CCTNAGG 1 cut(s) 441
BaeGI GKGCMC 1 cut(s) 850
BanI GGYRCC 1 cut(s) 457
BanII GRGCYC 3 cut(s) 217, 850, 1064
BarI GAAGNNNNNNTAC 2 cut(s) 798, 830
BbsI GAAGAC 5 cut(s) 99, 120, 129, 159, 1397
Bbv12I GWGCWC 2 cut(s) 1064, 1196
BbvCI CCTCAGC 1 cut(s) 1047
BbvI GCAGC 5 cut(s) 274, 761, 954, 1027, 1030
BccI CCATC 4 cut(s) 853, 937, 1258, 1424
BcgI CGANNNNNNTGC 2 cut(s) 721, 755
BciT130I CCWGG 1 cut(s) 451
BcnI CCSGG 1 cut(s) 1361
BcoDI GTCTC 6 cut(s) 14, 222, 306, 317, 493, 1308
BfaI CTAG 5 cut(s) 468, 474, 839, 1254, 1419
BfuAI ACCTGC 1 cut(s) 689
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 6 cut(s) 248, 288, 775, 968, 1041, 1044
BlpI GCTNAGC 1 cut(s) 694
BlsI GCNGC 6 cut(s) 249, 289, 776, 969, 1042, 1045
Bme1390I CCNGG 2 cut(s) 451, 1361
BmeRI GACNNNNNGTC 2 cut(s) 160, 337
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 3 cut(s) 245, 846, 847
BmiI GGNNCC 4 cut(s) 447, 459, 647, 848
BmrFI CCNGG 2 cut(s) 451, 1361
BmrI ACTGGG 2 cut(s) 67, 456
BmsI GCATC 4 cut(s) 492, 546, 759, 839
BmuI ACTGGG 2 cut(s) 67, 456
BpiI GAAGAC 5 cut(s) 99, 120, 129, 159, 1397
BplI GAGNNNNNCTC 2 cut(s) 286, 318
BpmI CTGGAG 1 cut(s) 1332
Bpu10I CCTNAGC 1 cut(s) 1047
Bpu1102I GCTNAGC 1 cut(s) 694
BpuEI CTTGAG 3 cut(s) 455, 980, 1289
BpuMI CCSGG 1 cut(s) 1361
BsaBI GATNNNNATC 1 cut(s) 22
BsaHI GRCGYC 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 3 cut(s) 366, 449, 915
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 2 cut(s) 372, 399
Bse1I ACTGG 5 cut(s) 73, 259, 462, 649, 1322
Bse21I CCTNAGG 1 cut(s) 441
Bse3DI GCAATG 2 cut(s) 688, 909
Bse8I GATNNNNATC 1 cut(s) 22
BseBI CCWGG 1 cut(s) 451
BseDI CCNNGG 3 cut(s) 366, 449, 915
BseGI GGATG 2 cut(s) 1158, 1435
BseJI GATNNNNATC 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 372, 399
BseMI GCAATG 2 cut(s) 688, 909
BseMII CTCAG 6 cut(s) 202, 284, 420, 432, 588, 1038
BseNI ACTGG 5 cut(s) 73, 259, 462, 649, 1322
BseRI GAGGAG 2 cut(s) 54, 594
BseSI GKGCMC 1 cut(s) 850
BseXI GCAGC 5 cut(s) 274, 761, 954, 1027, 1030
BsgI GTGCAG 1 cut(s) 778
Bsh1236I CGCG 1 cut(s) 1023
BshFI GGCC 3 cut(s) 247, 585, 848
BshNI GGYRCC 1 cut(s) 457
BsiHKAI GWGCWC 2 cut(s) 1064, 1196
BsiSI CCGG 1 cut(s) 1361
BslFI GGGAC 1 cut(s) 1131
BslI CCNNNNNNNGG 2 cut(s) 372, 399
BsmAI GTCTC 6 cut(s) 14, 222, 306, 317, 493, 1308
BsmBI CGTCTC 2 cut(s) 14, 317
BsmFI GGGAC 1 cut(s) 1131
BsmI GAATGC 1 cut(s) 904
BsnI GGCC 3 cut(s) 247, 585, 848
Bso31I GGTCTC 1 cut(s) 493
Bsp120I GGGCCC 1 cut(s) 846
Bsp1286I GDGCHC 4 cut(s) 217, 850, 1064, 1196
Bsp143I GATC 6 cut(s) 17, 267, 949, 1014, 1096, 1292
Bsp1720I GCTNAGC 1 cut(s) 694
Bsp19I CCATGG 1 cut(s) 915
BspACI CCGC 3 cut(s) 30, 248, 1342
BspANI GGCC 3 cut(s) 247, 585, 848
BspCNI CTCAG 6 cut(s) 201, 285, 421, 433, 587, 1039
BspFNI CGCG 1 cut(s) 1023
BspLI GGNNCC 4 cut(s) 447, 459, 647, 848
BspMI ACCTGC 1 cut(s) 689
BspPI GGATC 3 cut(s) 12, 944, 1009
BspQI GCTCTTC 1 cut(s) 627
BspT107I GGYRCC 1 cut(s) 457
BspTNI GGTCTC 1 cut(s) 493
BsrDI GCAATG 2 cut(s) 688, 909
BsrI ACTGG 5 cut(s) 73, 259, 462, 649, 1322
BssECI CCNNGG 3 cut(s) 366, 449, 915
BssMI GATC 6 cut(s) 17, 267, 949, 1014, 1096, 1292
BssNI GRCGYC 1 cut(s) 338
BssT1I CCWWGG 1 cut(s) 915
Bst2UI CCWGG 1 cut(s) 451
Bst4CI ACNGT 5 cut(s) 58, 239, 533, 1153, 1285
Bst6I CTCTTC 2 cut(s) 627, 1212
BstACI GRCGYC 1 cut(s) 338
BstAPI GCANNNNNTGC 3 cut(s) 464, 869, 976
BstC8I GCNNGC 5 cut(s) 904, 972, 1171, 1259, 1383
BstDEI CTNAG 9 cut(s) 188, 293, 429, 441, 495, 574, 694, 1047, 1386
BstDSI CCRYGG 1 cut(s) 915
BstENI CCTNNNNNAGG 1 cut(s) 397
BstF5I GGATG 2 cut(s) 1158, 1435
BstFNI CGCG 1 cut(s) 1023
BstHHI GCGC 3 cut(s) 774, 874, 1023
BstKTI GATC 6 cut(s) 20, 270, 952, 1017, 1099, 1295
BstMAI GTCTC 6 cut(s) 14, 222, 306, 317, 493, 1308
BstMBI GATC 6 cut(s) 17, 267, 949, 1014, 1096, 1292
BstNI CCWGG 1 cut(s) 451
BstNSI RCATGY 4 cut(s) 380, 389, 974, 1124
BstSCI CCNGG 2 cut(s) 449, 1359
BstSLI GKGCMC 1 cut(s) 850
BstUI CGCG 1 cut(s) 1023
BstV1I GCAGC 5 cut(s) 274, 761, 954, 1027, 1030
BstV2I GAAGAC 5 cut(s) 99, 120, 129, 159, 1397
BstX2I RGATCY 2 cut(s) 949, 1014
BstYI RGATCY 2 cut(s) 949, 1014
Bsu36I CCTNAGG 1 cut(s) 441
BsuRI GGCC 3 cut(s) 247, 585, 848
BtgI CCRYGG 1 cut(s) 915
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 2 cut(s) 1158, 1435
BtsI GCAGTG 5 cut(s) 322, 512, 833, 962, 981
BtsIMutI CAGTG 8 cut(s) 322, 469, 512, 538, 833, 962, 981, 1149
BveI ACCTGC 1 cut(s) 689
Cac8I GCNNGC 5 cut(s) 904, 972, 1171, 1259, 1383
CaiI CAGNNNCTG 1 cut(s) 1043
CfoI GCGC 3 cut(s) 774, 874, 1023
Cfr13I GGNCC 3 cut(s) 245, 846, 847
CseI GACGC 1 cut(s) 327
Csp6I GTAC 1 cut(s) 571
CspCI CAANNNNNGTGG 2 cut(s) 1134, 1169
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 9 cut(s) 188, 293, 429, 441, 495, 574, 694, 1047, 1386
DpnI GATC 6 cut(s) 19, 269, 951, 1016, 1098, 1294
DpnII GATC 6 cut(s) 17, 267, 949, 1014, 1096, 1292
DraIII CACNNNGTG 1 cut(s) 464
DriI GACNNNNNGTC 2 cut(s) 160, 337
Eam1104I CTCTTC 2 cut(s) 627, 1212
Eam1105I GACNNNNNGTC 2 cut(s) 160, 337
EarI CTCTTC 2 cut(s) 627, 1212
Ecl136II GAGCTC 1 cut(s) 1062
Eco130I CCWWGG 1 cut(s) 915
Eco147I AGGCCT 1 cut(s) 585
Eco24I GRGCYC 3 cut(s) 217, 850, 1064
Eco31I GGTCTC 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 1062
Eco57I CTGAAG 1 cut(s) 1185
Eco81I CCTNAGG 1 cut(s) 441
EcoICRI GAGCTC 1 cut(s) 1062
EcoNI CCTNNNNNAGG 1 cut(s) 397
EcoO109I RGGNCCY 1 cut(s) 847
EcoRII CCWGG 1 cut(s) 449
EcoT14I CCWWGG 1 cut(s) 915
EcoT38I GRGCYC 3 cut(s) 217, 850, 1064
ErhI CCWWGG 1 cut(s) 915
Esp3I CGTCTC 2 cut(s) 14, 317
FaqI GGGAC 1 cut(s) 1131
FblI GTMKAC 1 cut(s) 176
Fnu4HI GCNGC 6 cut(s) 248, 288, 775, 968, 1041, 1044
FokI GGATG 2 cut(s) 1145, 1442
FriOI GRGCYC 3 cut(s) 217, 850, 1064
Fsp4HI GCNGC 6 cut(s) 248, 288, 775, 968, 1041, 1044
FspBI CTAG 5 cut(s) 468, 474, 839, 1254, 1419
FspI TGCGCA 1 cut(s) 873
GlaI GCGC 3 cut(s) 773, 873, 1022
GluI GCNGC 6 cut(s) 248, 288, 775, 968, 1041, 1044
GsuI CTGGAG 1 cut(s) 1332
HaeIII GGCC 3 cut(s) 247, 585, 848
HapII CCGG 1 cut(s) 1361
HgaI GACGC 1 cut(s) 327
HhaI GCGC 3 cut(s) 774, 874, 1023
Hin1I GRCGYC 1 cut(s) 338
Hin6I GCGC 3 cut(s) 772, 872, 1021
HinP1I GCGC 3 cut(s) 772, 872, 1021
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 1437
HinfI GANTC 7 cut(s) 33, 42, 161, 746, 1034, 1229, 1235
HpaII CCGG 1 cut(s) 1361
HphI GGTGA 5 cut(s) 37, 46, 351, 382, 778
Hpy166II GTNNAC 4 cut(s) 54, 177, 359, 1156
Hpy188III TCNNGA 5 cut(s) 15, 227, 576, 997, 1127
Hpy8I GTNNAC 4 cut(s) 54, 177, 359, 1156
Hpy99I CGWCG 4 cut(s) 41, 62, 161, 335
HpyAV CCTTC 2 cut(s) 448, 590
HpyCH4III ACNGT 5 cut(s) 58, 239, 533, 1153, 1285
HpyCH4IV ACGT 1 cut(s) 333
HpyF3I CTNAG 9 cut(s) 188, 293, 429, 441, 495, 574, 694, 1047, 1386
HpySE526I ACGT 1 cut(s) 333
Hsp92I GRCGYC 1 cut(s) 338
HspAI GCGC 3 cut(s) 772, 872, 1021
Kzo9I GATC 6 cut(s) 17, 267, 949, 1014, 1096, 1292
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 2 cut(s) 67, 931
Lsp1109I GCAGC 5 cut(s) 274, 761, 954, 1027, 1030
LweI GCATC 4 cut(s) 492, 546, 759, 839
MaeI CTAG 5 cut(s) 468, 474, 839, 1254, 1419
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 2 cut(s) 388, 810
MalI GATC 6 cut(s) 19, 269, 951, 1016, 1098, 1294
MboI GATC 6 cut(s) 17, 267, 949, 1014, 1096, 1292
MflI RGATCY 2 cut(s) 949, 1014
MhlI GDGCHC 4 cut(s) 217, 850, 1064, 1196
MluCI AATT 4 cut(s) 140, 678, 853, 988
MlyI GAGTC 3 cut(s) 27, 36, 170
MmeI TCCRAC 3 cut(s) 19, 92, 755
MseI TTAA 7 cut(s) 234, 405, 588, 704, 956, 1010, 1322
MslI CAYNNNNRTG 2 cut(s) 914, 1092
MspA1I CMGCKG 1 cut(s) 1040
MspI CCGG 1 cut(s) 1361
MspR9I CCNGG 2 cut(s) 451, 1361
Mva1269I GAATGC 1 cut(s) 904
MvaI CCWGG 1 cut(s) 451
MvnI CGCG 1 cut(s) 1023
NciI CCSGG 1 cut(s) 1361
NcoI CCATGG 1 cut(s) 915
NdeII GATC 6 cut(s) 17, 267, 949, 1014, 1096, 1292
NlaIV GGNNCC 4 cut(s) 447, 459, 647, 848
NmuCI GTSAC 2 cut(s) 388, 810
NsbI TGCGCA 1 cut(s) 873
NspI RCATGY 4 cut(s) 380, 389, 974, 1124
PaeI GCATGC 1 cut(s) 974
PceI AGGCCT 1 cut(s) 585
PciI ACATGT 1 cut(s) 1120
PciSI GCTCTTC 1 cut(s) 627
PcsI WCGNNNNNNNCGW 3 cut(s) 13, 45, 123
PctI GAATGC 1 cut(s) 904
PfeI GAWTC 4 cut(s) 746, 1034, 1229, 1235
PkrI GCNGC 6 cut(s) 249, 289, 776, 969, 1042, 1045
PleI GAGTC 3 cut(s) 27, 36, 169
PpsI GAGTC 3 cut(s) 27, 36, 169
PscI ACATGT 1 cut(s) 1120
Psp124BI GAGCTC 1 cut(s) 1064
Psp6I CCWGG 1 cut(s) 449
PspGI CCWGG 1 cut(s) 449
PspN4I GGNNCC 4 cut(s) 447, 459, 647, 848
PspOMI GGGCCC 1 cut(s) 846
PspPI GGNCC 3 cut(s) 245, 846, 847
PstNI CAGNNNCTG 1 cut(s) 1043
PsuI RGATCY 2 cut(s) 949, 1014
PvuII CAGCTG 1 cut(s) 1040
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
RseI CAYNNNNRTG 2 cut(s) 914, 1092
SacI GAGCTC 1 cut(s) 1064
SalI GTCGAC 1 cut(s) 175
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 7 cut(s) 234, 405, 588, 704, 956, 1010, 1322
SatI GCNGC 6 cut(s) 248, 288, 775, 968, 1041, 1044
Sau3AI GATC 6 cut(s) 17, 267, 949, 1014, 1096, 1292
Sau96I GGNCC 3 cut(s) 245, 846, 847
SchI GAGTC 3 cut(s) 27, 36, 170
ScrFI CCNGG 2 cut(s) 451, 1361
SduI GDGCHC 4 cut(s) 217, 850, 1064, 1196
SfaNI GCATC 4 cut(s) 492, 546, 759, 839
SmiMI CAYNNNNRTG 2 cut(s) 914, 1092
SmlI CTYRAG 3 cut(s) 434, 995, 1268
SmoI CTYRAG 3 cut(s) 434, 995, 1268
SphI GCATGC 1 cut(s) 974
Sse9I AATT 4 cut(s) 140, 678, 853, 988
SseBI AGGCCT 1 cut(s) 585
SsiI CCGC 3 cut(s) 30, 248, 1342
SspI AATATT 1 cut(s) 603
SspMI CTAG 5 cut(s) 468, 474, 839, 1254, 1419
SstI GAGCTC 1 cut(s) 1064
StuI AGGCCT 1 cut(s) 585
StyD4I CCNGG 2 cut(s) 449, 1359
StyI CCWWGG 1 cut(s) 915
TaaI ACNGT 5 cut(s) 58, 239, 533, 1153, 1285
TaiI ACGT 1 cut(s) 336
TaqI TCGA 4 cut(s) 176, 226, 500, 1227
TasI AATT 4 cut(s) 140, 678, 853, 988
TatI WGTACW 1 cut(s) 570
TauI GCSGC 1 cut(s) 250
TfiI GAWTC 4 cut(s) 746, 1034, 1229, 1235
Tru1I TTAA 7 cut(s) 234, 405, 588, 704, 956, 1010, 1322
Tru9I TTAA 7 cut(s) 234, 405, 588, 704, 956, 1010, 1322
TscAI CASTG 8 cut(s) 322, 469, 512, 538, 840, 969, 981, 1156
TseFI GTSAC 2 cut(s) 388, 810
TseI GCWGC 5 cut(s) 287, 774, 967, 1040, 1043
Tsp45I GTSAC 2 cut(s) 388, 810
TspDTI ATGAA 8 cut(s) 105, 162, 677, 894, 911, 1001, 1278, 1320
TspGWI ACGGA 2 cut(s) 341, 839
TspRI CASTG 8 cut(s) 322, 469, 512, 538, 840, 969, 981, 1156
XagI CCTNNNNNAGG 1 cut(s) 397
XapI RAATTY 2 cut(s) 140, 988
XceI RCATGY 4 cut(s) 380, 389, 974, 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 656
XmiI GTMKAC 1 cut(s) 176
XspI CTAG 5 cut(s) 468, 474, 839, 1254, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.