Rroxscaffold_4G00295800

P21-Rho-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014369
Physical Location & Seq
Reverse (-)
15875378 .. 15879576
4199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00295800.1

Sequence Viewer

Length: 1455 bp
ATGGCTCGTCTCTTCCGATCCAAATCTTGCGGACTCGTCGGACTCACCGAGTTCACCGTCGCTCCTCCGACCCAGTTCTGCCAAAACCATGAAGACGAAACAGAGAGAGAAGAAGAAGAGGAAGACGAAAAAGGGTCTGAATTTGATGAAGAAGACGAAGAAGAGGAATCGTGTGAGGTGTCGACCCCATTTCTCAGTCCTATGAGCAGAGCCAATGGGACTCATAAAGGTCGAGACTTTAACAGTAGTGGGCCGCAGAACAACCAGTTTGCGATCATGGATATTCTGGTGGCTGCTCTGAGGAAGTCTCTGATTACTTGCAGCGTCGAGACGGACGATGTGGTGTCCTCCTTTGATATAAGTTCACCTACCGAGGTGAGGCATGTTTCGCATGTGACCTTTGATAGGTTTAATGGGTTTCTGGGTTTGCCTACGGAGCTTGAGCCTGAGGTTCCCAGGAAGGCACCTAGTGCTAGTGCTAGTGTGTTTGGGGTCTCTGCTAAGTCGATGCAGTGTTCTTATGATGATAGAGGAAACAGTGTGCCAACAATACTTCTCATGATGCAAAAGCGATTGTACTCAGGAGGAGGTCTTAAAGCAGAAGGAATATTCAGAATAAATGCTGAAAATAGCCAAGAAGAGCGCGTTCGGAACCAGTTGAATAGTGGTGTAGTGCCACATGGAATTGATGTTCATTGCTTAGCAGGTTTAATAAAGGCATGGTTTAGAGAGCTTCCGACACGGGTACTTGATTCTCTTACACCAGAACAGGTGATGCGCTGCAACACAGAAGATGACTGCACAGGACTCGTAAAGTCACTTCCTGCAACGGAAGCATCACTGCTAGATTGGGCCCTCAATTTGATGGCAGATGTTGCGCAGAATGAACAACACAATAAGATGAACGCTCGCAACATTGCCATGGTTTTTGCTCCTAACATGACAGAGATGGCAGATCCTTTAACAGCACTGCTACATGCAGTGCAAGTTATGAATTTCCTCAAGACACTAATCTTAAAGATCCTGCGCGAAAGAGAGGAATCAGCTGCTGCTGAGGCTTTGCAGAGGTCATCTTGCTCAAAATCTCCCAACCACAACTATGATCTTCCTTATTCCATTATGGGACATGTTCAGGAGAACTCATTGAGGACAACCACTATGGACAGCCCTGAAGCCTGCATTGACGAGAAACTTTGGAGCTCTCCTGTAATGAGTGATGAGGAAGAGGAAATCGAATCCGATTCCAATAGTAGCCCATATAGCAAGCATGAACTTGAGTGTTCGGAAACTGTTTGTAGATCAGGTGGATATGAAGCTGGAGACTGGTTAAGTTTGAGGAAAGGAGTGCGGAAGCTGTGCAGCCACCCGGTCTTTCAGTTGAGTAAGCCGGCTAAGAAGACTGCAAATGTTGGGATTGTAAATACTAGGGGAGGAGATGGAGAAGCTTGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0008150 GO:0009505 GO:0009531 GO:0009664 GO:0009987 GO:0016020 GO:0016043 GO:0030312 GO:0044464 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

484

Amino Acids

53.57

Weight (kDa)

4.85

Isoelectric Point (pI)

59.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 182 - 316 1.2e-22 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46710
fragaria_vesca FvH4_7g17360
malus_domestica MD01G1086700.v1.1 MD07G1156800.v1.1
prunus_persica Prupe.2G195100_v2.0.a1
pyrus_communis pycom01g11060 pycom07g15340
rosa_chinensis RchiOBHm_Chr1g0360801
rosa_laevigata RLG00000027785
rosa_multiflora Rmu_ssc0000022.1_g000034
rosa_roxburghii Rroxscaffold_4G00295800
rosa_rugosa Rorug01G0281600
rosa_samantha Rh1AG293600 Rh1BG258200 Rh1CG276100 Rh1DG288500
rosa_wichuraiana Rw1G025980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 879
Acc36I ACCTGC 1 cut(s) 695
AccB1I GGYRCC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 182
AccII CGCG 2 cut(s) 645, 1029
AciI CCGC 3 cut(s) 30, 254, 1348
AclWI GGATC 3 cut(s) 12, 950, 1015
AcsI RAATTY 2 cut(s) 140, 994
AcuI CTGAAG 1 cut(s) 1191
AdeI CACNNNGTG 1 cut(s) 470
AfaI GTAC 2 cut(s) 578, 747
AfiI CCNNNNNNNGG 2 cut(s) 378, 405
AflIII ACRYGT 1 cut(s) 1126
AgsI TTSAA 1 cut(s) 661
AjnI CCWGG 1 cut(s) 455
AloI GAACNNNNNNTCC 2 cut(s) 675, 707
AluBI AGCT 7 cut(s) 439, 733, 1046, 1200, 1316, 1354, 1445
AluI AGCT 7 cut(s) 439, 733, 1046, 1200, 1316, 1354, 1445
Alw21I GWGCWC 1 cut(s) 1202
Alw26I GTCTC 6 cut(s) 14, 228, 312, 323, 499, 1314
AlwI GGATC 3 cut(s) 12, 950, 1015
AlwNI CAGNNNCTG 1 cut(s) 1049
AoxI GGCC 2 cut(s) 251, 852
ApaI GGGCCC 1 cut(s) 856
ApeKI GCWGC 6 cut(s) 293, 321, 780, 1046, 1049, 1359
ApoI RAATTY 2 cut(s) 140, 994
AspLEI GCGC 4 cut(s) 645, 780, 880, 1029
AspS9I GGNCC 3 cut(s) 251, 852, 853
AsuC2I CCSGG 1 cut(s) 1367
AsuHPI GGTGA 5 cut(s) 37, 46, 357, 388, 784
AxyI CCTNAGG 1 cut(s) 447
BaeGI GKGCMC 1 cut(s) 856
BanI GGYRCC 1 cut(s) 463
BanII GRGCYC 2 cut(s) 856, 1202
BarI GAAGNNNNNNTAC 2 cut(s) 804, 836
BbsI GAAGAC 4 cut(s) 99, 129, 159, 1403
Bbv12I GWGCWC 1 cut(s) 1202
BbvCI CCTCAGC 1 cut(s) 1053
BbvI GCAGC 6 cut(s) 280, 333, 767, 1033, 1036, 1371
BccI CCATC 3 cut(s) 859, 943, 1430
BcgI CGANNNNNNTGC 2 cut(s) 790, 824
BciT130I CCWGG 1 cut(s) 457
BcnI CCSGG 1 cut(s) 1367
BcoDI GTCTC 6 cut(s) 14, 228, 312, 323, 499, 1314
BfaI CTAG 5 cut(s) 468, 474, 480, 845, 1425
BfuAI ACCTGC 1 cut(s) 695
BisI GCNGC 7 cut(s) 254, 294, 322, 781, 1047, 1050, 1360
BlpI GCTNAGC 1 cut(s) 700
BlsI GCNGC 7 cut(s) 255, 295, 323, 782, 1048, 1051, 1361
Bme1390I CCNGG 2 cut(s) 457, 1367
BmgT120I GGNCC 3 cut(s) 251, 852, 853
BmiI GGNNCC 4 cut(s) 453, 465, 653, 854
BmrFI CCNGG 2 cut(s) 457, 1367
BmrI ACTGGG 1 cut(s) 67
BmsI GCATC 4 cut(s) 498, 552, 765, 845
BmuI ACTGGG 1 cut(s) 67
BpiI GAAGAC 4 cut(s) 99, 129, 159, 1403
BplI GAGNNNNNCTC 2 cut(s) 292, 324
BpmI CTGGAG 1 cut(s) 1338
Bpu10I CCTNAGC 1 cut(s) 1053
Bpu1102I GCTNAGC 1 cut(s) 700
BpuEI CTTGAG 3 cut(s) 461, 986, 1295
BpuMI CCSGG 1 cut(s) 1367
BsaBI GATNNNNATC 1 cut(s) 22
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 3 cut(s) 372, 455, 921
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 2 cut(s) 378, 405
Bse118I RCCGGY 1 cut(s) 1387
Bse1I ACTGG 4 cut(s) 73, 265, 655, 1328
Bse21I CCTNAGG 1 cut(s) 447
Bse3DI GCAATG 2 cut(s) 694, 915
Bse8I GATNNNNATC 1 cut(s) 22
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 3 cut(s) 372, 455, 921
BseJI GATNNNNATC 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 378, 405
BseMI GCAATG 2 cut(s) 694, 915
BseMII CTCAG 5 cut(s) 208, 290, 438, 594, 1044
BseNI ACTGG 4 cut(s) 73, 265, 655, 1328
BseRI GAGGAG 3 cut(s) 54, 600, 1446
BseSI GKGCMC 1 cut(s) 856
BseXI GCAGC 6 cut(s) 280, 333, 767, 1033, 1036, 1371
BsgI GTGCAG 2 cut(s) 784, 1378
Bsh1236I CGCG 2 cut(s) 645, 1029
BshFI GGCC 2 cut(s) 253, 854
BshNI GGYRCC 1 cut(s) 463
BsiHKAI GWGCWC 1 cut(s) 1202
BsiSI CCGG 2 cut(s) 1367, 1388
BslFI GGGAC 2 cut(s) 232, 1137
BslI CCNNNNNNNGG 2 cut(s) 378, 405
BsmAI GTCTC 6 cut(s) 14, 228, 312, 323, 499, 1314
BsmBI CGTCTC 2 cut(s) 14, 323
BsmFI GGGAC 2 cut(s) 232, 1137
BsnI GGCC 2 cut(s) 253, 854
Bso31I GGTCTC 1 cut(s) 499
Bsp120I GGGCCC 1 cut(s) 852
Bsp1286I GDGCHC 2 cut(s) 856, 1202
Bsp143I GATC 6 cut(s) 17, 273, 955, 1020, 1102, 1298
Bsp1720I GCTNAGC 1 cut(s) 700
Bsp19I CCATGG 1 cut(s) 921
BspACI CCGC 3 cut(s) 30, 254, 1348
BspANI GGCC 2 cut(s) 253, 854
BspCNI CTCAG 5 cut(s) 207, 291, 439, 593, 1045
BspFNI CGCG 2 cut(s) 645, 1029
BspHI TCATGA 1 cut(s) 558
BspLI GGNNCC 4 cut(s) 453, 465, 653, 854
BspMI ACCTGC 1 cut(s) 695
BspPI GGATC 3 cut(s) 12, 950, 1015
BspQI GCTCTTC 1 cut(s) 633
BspT107I GGYRCC 1 cut(s) 463
BspTNI GGTCTC 1 cut(s) 499
BsrDI GCAATG 2 cut(s) 694, 915
BsrFI RCCGGY 1 cut(s) 1387
BsrI ACTGG 4 cut(s) 73, 265, 655, 1328
BssAI RCCGGY 1 cut(s) 1387
BssECI CCNNGG 3 cut(s) 372, 455, 921
BssMI GATC 6 cut(s) 17, 273, 955, 1020, 1102, 1298
BssT1I CCWWGG 1 cut(s) 921
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 4 cut(s) 58, 245, 539, 1291
Bst6I CTCTTC 5 cut(s) 17, 111, 156, 633, 1218
BstAPI GCANNNNNTGC 2 cut(s) 470, 875
BstC8I GCNNGC 4 cut(s) 910, 1177, 1265, 1389
BstDEI CTNAG 8 cut(s) 194, 299, 447, 501, 580, 700, 1053, 1392
BstDSI CCRYGG 1 cut(s) 921
BstENI CCTNNNNNAGG 1 cut(s) 403
BstFNI CGCG 2 cut(s) 645, 1029
BstHHI GCGC 4 cut(s) 645, 780, 880, 1029
BstKTI GATC 6 cut(s) 20, 276, 958, 1023, 1105, 1301
BstMAI GTCTC 6 cut(s) 14, 228, 312, 323, 499, 1314
BstMBI GATC 6 cut(s) 17, 273, 955, 1020, 1102, 1298
BstMWI GCNNNNNNNGC 7 cut(s) 388, 436, 470, 833, 875, 1055, 1260
BstNI CCWGG 1 cut(s) 457
BstNSI RCATGY 4 cut(s) 386, 395, 980, 1130
BstSCI CCNGG 2 cut(s) 455, 1365
BstSLI GKGCMC 1 cut(s) 856
BstUI CGCG 2 cut(s) 645, 1029
BstV1I GCAGC 6 cut(s) 280, 333, 767, 1033, 1036, 1371
BstV2I GAAGAC 4 cut(s) 99, 129, 159, 1403
BstX2I RGATCY 2 cut(s) 955, 1020
BstYI RGATCY 2 cut(s) 955, 1020
Bsu36I CCTNAGG 1 cut(s) 447
BsuRI GGCC 2 cut(s) 253, 854
BtgI CCRYGG 1 cut(s) 921
BtsI GCAGTG 4 cut(s) 518, 839, 968, 987
BtsIMutI CAGTG 5 cut(s) 518, 544, 839, 968, 987
BveI ACCTGC 1 cut(s) 695
Cac8I GCNNGC 4 cut(s) 910, 1177, 1265, 1389
CaiI CAGNNNCTG 1 cut(s) 1049
CciI TCATGA 1 cut(s) 558
CfoI GCGC 4 cut(s) 645, 780, 880, 1029
Cfr10I RCCGGY 1 cut(s) 1387
Cfr13I GGNCC 3 cut(s) 251, 852, 853
CseI GACGC 1 cut(s) 313
Csp6I GTAC 2 cut(s) 577, 746
CviQI GTAC 2 cut(s) 577, 746
DdeI CTNAG 8 cut(s) 194, 299, 447, 501, 580, 700, 1053, 1392
DpnI GATC 6 cut(s) 19, 275, 957, 1022, 1104, 1300
DpnII GATC 6 cut(s) 17, 273, 955, 1020, 1102, 1298
DraIII CACNNNGTG 1 cut(s) 470
Eam1104I CTCTTC 5 cut(s) 17, 111, 156, 633, 1218
EarI CTCTTC 5 cut(s) 17, 111, 156, 633, 1218
Ecl136II GAGCTC 1 cut(s) 1200
Eco130I CCWWGG 1 cut(s) 921
Eco24I GRGCYC 2 cut(s) 856, 1202
Eco31I GGTCTC 1 cut(s) 499
Eco53kI GAGCTC 1 cut(s) 1200
Eco57I CTGAAG 1 cut(s) 1191
Eco81I CCTNAGG 1 cut(s) 447
EcoICRI GAGCTC 1 cut(s) 1200
EcoNI CCTNNNNNAGG 1 cut(s) 403
EcoO109I RGGNCCY 1 cut(s) 853
EcoRII CCWGG 1 cut(s) 455
EcoT14I CCWWGG 1 cut(s) 921
EcoT38I GRGCYC 2 cut(s) 856, 1202
ErhI CCWWGG 1 cut(s) 921
Esp3I CGTCTC 2 cut(s) 14, 323
FaqI GGGAC 2 cut(s) 232, 1137
FblI GTMKAC 1 cut(s) 182
Fnu4HI GCNGC 7 cut(s) 254, 294, 322, 781, 1047, 1050, 1360
FriOI GRGCYC 2 cut(s) 856, 1202
Fsp4HI GCNGC 7 cut(s) 254, 294, 322, 781, 1047, 1050, 1360
FspBI CTAG 5 cut(s) 468, 474, 480, 845, 1425
FspI TGCGCA 1 cut(s) 879
GlaI GCGC 4 cut(s) 644, 779, 879, 1028
GluI GCNGC 7 cut(s) 254, 294, 322, 781, 1047, 1050, 1360
GsuI CTGGAG 1 cut(s) 1338
HaeIII GGCC 2 cut(s) 253, 854
HapII CCGG 2 cut(s) 1367, 1388
HgaI GACGC 1 cut(s) 313
HhaI GCGC 4 cut(s) 645, 780, 880, 1029
Hin6I GCGC 4 cut(s) 643, 778, 878, 1027
HinP1I GCGC 4 cut(s) 643, 778, 878, 1027
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HindIII AAGCTT 1 cut(s) 1443
HinfI GANTC 9 cut(s) 33, 42, 167, 220, 752, 807, 1040, 1235, 1241
HpaII CCGG 2 cut(s) 1367, 1388
HphI GGTGA 5 cut(s) 37, 46, 357, 388, 784
Hpy166II GTNNAC 3 cut(s) 54, 183, 365
Hpy188III TCNNGA 6 cut(s) 233, 328, 559, 582, 1003, 1133
Hpy8I GTNNAC 3 cut(s) 54, 183, 365
Hpy99I CGWCG 3 cut(s) 41, 62, 329
HpyAV CCTTC 2 cut(s) 454, 596
HpyCH4III ACNGT 4 cut(s) 58, 245, 539, 1291
HpyF10VI GCNNNNNNNGC 7 cut(s) 388, 436, 470, 833, 875, 1055, 1260
HpyF3I CTNAG 8 cut(s) 194, 299, 447, 501, 580, 700, 1053, 1392
HspAI GCGC 4 cut(s) 643, 778, 878, 1027
KroI GCCGGC 1 cut(s) 1387
KroNI GCCGGC 1 cut(s) 1389
Kzo9I GATC 6 cut(s) 17, 273, 955, 1020, 1102, 1298
LguI GCTCTTC 1 cut(s) 633
LmnI GCTCC 4 cut(s) 67, 436, 937, 1197
Lsp1109I GCAGC 6 cut(s) 280, 333, 767, 1033, 1036, 1371
LweI GCATC 4 cut(s) 498, 552, 765, 845
MaeI CTAG 5 cut(s) 468, 474, 480, 845, 1425
MaeIII GTNAC 2 cut(s) 394, 816
MalI GATC 6 cut(s) 19, 275, 957, 1022, 1104, 1300
MboI GATC 6 cut(s) 17, 273, 955, 1020, 1102, 1298
MflI RGATCY 2 cut(s) 955, 1020
MhlI GDGCHC 2 cut(s) 856, 1202
MluCI AATT 4 cut(s) 140, 684, 859, 994
MlyI GAGTC 4 cut(s) 27, 36, 214, 801
MmeI TCCRAC 3 cut(s) 19, 92, 761
MroNI GCCGGC 1 cut(s) 1387
MseI TTAA 7 cut(s) 240, 411, 594, 710, 962, 1016, 1328
MslI CAYNNNNRTG 2 cut(s) 920, 1098
MspA1I CMGCKG 1 cut(s) 1046
MspI CCGG 2 cut(s) 1367, 1388
MspR9I CCNGG 2 cut(s) 457, 1367
MvaI CCWGG 1 cut(s) 457
MvnI CGCG 2 cut(s) 645, 1029
MwoI GCNNNNNNNGC 7 cut(s) 388, 436, 470, 833, 875, 1055, 1260
NaeI GCCGGC 1 cut(s) 1389
NciI CCSGG 1 cut(s) 1367
NcoI CCATGG 1 cut(s) 921
NdeII GATC 6 cut(s) 17, 273, 955, 1020, 1102, 1298
NgoMIV GCCGGC 1 cut(s) 1387
NlaIV GGNNCC 4 cut(s) 453, 465, 653, 854
NmuCI GTSAC 2 cut(s) 394, 816
NsbI TGCGCA 1 cut(s) 879
NspI RCATGY 4 cut(s) 386, 395, 980, 1130
PagI TCATGA 1 cut(s) 558
PciI ACATGT 1 cut(s) 1126
PciSI GCTCTTC 1 cut(s) 633
PcsI WCGNNNNNNNCGW 3 cut(s) 13, 45, 333
PdiI GCCGGC 1 cut(s) 1389
PfeI GAWTC 5 cut(s) 167, 752, 1040, 1235, 1241
PkrI GCNGC 7 cut(s) 255, 295, 323, 782, 1048, 1051, 1361
PleI GAGTC 4 cut(s) 27, 36, 214, 801
PpsI GAGTC 4 cut(s) 27, 36, 214, 801
PscI ACATGT 1 cut(s) 1126
Psp124BI GAGCTC 1 cut(s) 1202
Psp6I CCWGG 1 cut(s) 455
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 4 cut(s) 453, 465, 653, 854
PspOMI GGGCCC 1 cut(s) 852
PspPI GGNCC 3 cut(s) 251, 852, 853
PstNI CAGNNNCTG 1 cut(s) 1049
PsuI RGATCY 2 cut(s) 955, 1020
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 2 cut(s) 578, 747
RsaNI GTAC 2 cut(s) 577, 746
RseI CAYNNNNRTG 2 cut(s) 920, 1098
SacI GAGCTC 1 cut(s) 1202
SalI GTCGAC 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 633
SaqAI TTAA 7 cut(s) 240, 411, 594, 710, 962, 1016, 1328
SatI GCNGC 7 cut(s) 254, 294, 322, 781, 1047, 1050, 1360
Sau3AI GATC 6 cut(s) 17, 273, 955, 1020, 1102, 1298
Sau96I GGNCC 3 cut(s) 251, 852, 853
SchI GAGTC 4 cut(s) 27, 36, 214, 801
ScrFI CCNGG 2 cut(s) 457, 1367
SduI GDGCHC 2 cut(s) 856, 1202
SfaNI GCATC 4 cut(s) 498, 552, 765, 845
SmiMI CAYNNNNRTG 2 cut(s) 920, 1098
SmlI CTYRAG 3 cut(s) 440, 1001, 1274
SmoI CTYRAG 3 cut(s) 440, 1001, 1274
Sse9I AATT 4 cut(s) 140, 684, 859, 994
SsiI CCGC 3 cut(s) 30, 254, 1348
SspI AATATT 1 cut(s) 609
SspMI CTAG 5 cut(s) 468, 474, 480, 845, 1425
SstI GAGCTC 1 cut(s) 1202
StyD4I CCNGG 2 cut(s) 455, 1365
StyI CCWWGG 1 cut(s) 921
TaaI ACNGT 4 cut(s) 58, 245, 539, 1291
TaqI TCGA 5 cut(s) 182, 232, 327, 506, 1233
TasI AATT 4 cut(s) 140, 684, 859, 994
TatI WGTACW 1 cut(s) 576
TauI GCSGC 1 cut(s) 256
TfiI GAWTC 5 cut(s) 167, 752, 1040, 1235, 1241
Tru1I TTAA 7 cut(s) 240, 411, 594, 710, 962, 1016, 1328
Tru9I TTAA 7 cut(s) 240, 411, 594, 710, 962, 1016, 1328
TscAI CASTG 5 cut(s) 518, 544, 846, 975, 987
TseFI GTSAC 2 cut(s) 394, 816
TseI GCWGC 6 cut(s) 293, 321, 780, 1046, 1049, 1359
Tsp45I GTSAC 2 cut(s) 394, 816
TspDTI ATGAA 8 cut(s) 105, 162, 683, 900, 917, 1007, 1284, 1326
TspGWI ACGGA 3 cut(s) 347, 449, 845
TspRI CASTG 5 cut(s) 518, 544, 846, 975, 987
XagI CCTNNNNNAGG 1 cut(s) 403
XapI RAATTY 2 cut(s) 140, 994
XceI RCATGY 4 cut(s) 386, 395, 980, 1130
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XmiI GTMKAC 1 cut(s) 182
XspI CTAG 5 cut(s) 468, 474, 480, 845, 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.