MD01G1119300.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016938
Physical Location & Seq
Reverse (-)
23333010 .. 23334419
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1119300.v1.1.491

Sequence Viewer

Length: 1410 bp
ATGGAGCTCTTGTTAGCAATGTGTTTATTGCTTATATTCTTTGCTTGCATCTACCTGTGTGAGTTGCTGCTTCAATGGAGGCACCAACAATGCTACTTATTAGCCTATGAGTGCTACAAGGCCACAGACGAGCGGAAGCTGGACACCGAATCCTGCGTCAAGATCATCCGCCGCAACACAAATCTGGGGCTGGAAGAATACAGGTTTCTCCTCAAAACCATCGTTAACTCGGGCCTTGGAGAGGAAACCTACGGTCCAAGAAATGTCATCGCCGGCTGCGAAGACTGTCCTAGTTTGGAAGACTCACTGTGGGAGATGGACGACATAATGTTTGGCACGCTTGACAAGCTATTCCACAAAACAGGAATTTCACCTTCAAAAATTGACATACTAGTGGTCAACGTGTCGTTGTTCTCTCCTGCACCTTCTCTAACGGCTAGGATCATAAACCGTTACAAGATGAAGACGGACATCAAGGCGTTCAACCTCTCGGGGATGGGGTGCAGTGGAAGCCTCGTGGCCATTGATCTTGTGCAGAACTTGTTCAAGACGTACAAGAATGTAAATGCCGTCCTTGTCAGCACAGAATCGCTTGCTCCGAATTGGTACCGCGGCAAAGTTCAGCCCATGATGCTTGCTAACTGCCTGTATCGCTCAGGAGGGTGTTCTATGATCTTTACAAACAAAATGCATCTCAAGCATCGATCACTTTTGAAATTGCAATGCATCGTGCGAACGCATCTTGGTTCACTCGATGAAGGATATGGCTGCTCCATGCAAGTAGAAGATGAAAATGGATTCCGTGGTTTTAATCTCAGCAAAAGCATAGCCAAAGTAGCAGCCAAAGCTCTTACCATGAACCTTCGAATCCTACTGCGAAAAGTGTTACCGCTTACAGAATTACTTCGCTATGTTATAGTGTCAAGCCGTCAAAACAAAGTTGCAAATGCTGGCTTGAATCTCAAGGCAGGACTTGATCATTTTTGCATACCCCCAACAGAAGCTGCAGTTGTAAATGGGGTTGGAAAGAGCTTAGGACTCAACGACTATGATCTCGAGCCTGTGCGAATGGCGCTGCACCGATTTGGGAACACGTCTTCGGGTGGGCTTTGGTATGTTTTGGGGTACATGGAGGCTAAGAGGAGGCTTAAAAAGGGTGAGAAAATTCTTATGGTGAGCTTTGGAGCGGGCTATGAGTGCAACAATTGTGTGTGGGAGATTTTGAGGGACATGGAAAATGCCAATGTGTGGAAAGATTGCATAGAAACTTACCCTCCAGAAACCATAGTGAATCCTTTCATGGAGAAGTATAGCTGGATCAACGATGAATATTTGAGCTTTGTCAGATTTGAGGAGGTTATGAATGCAATTTGTGTTGATGGTCAACGCTTTCAGGAGAGGCATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

470

Amino Acids

53.21

Weight (kDa)

8.19

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 26 - 307 9.9e-110 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 324 - 406 3.1e-09 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016938)

Species Orthologous Gene IDs
malus_domestica MD01G1119300.v1.1 MD07G1188100.v1.1
prunus_persica Prupe.2G226000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365261
rosa_laevigata RLG00000027436
rosa_multiflora Rmu_sc0020532.1_g000001
rosa_roxburghii Rroxscaffold_4G00291340 Rroxscaffold_4G00291880
rosa_rugosa Rorug01G0320500
rosa_samantha Rh1AG329500 Rh1BG290900 Rh1CG307800 Rh1DG322600
rosa_wichuraiana Rw1G029220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 606
AccB1I GGYRCC 2 cut(s) 81, 606
AccB7I CCANNNNNTGG 1 cut(s) 1248
AccBSI CCGCTC 2 cut(s) 133, 1187
AccII CGCG 1 cut(s) 612
AciI CCGC 7 cut(s) 133, 169, 172, 610, 612, 890, 1187
AclWI GGATC 2 cut(s) 449, 1325
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 2 cut(s) 366, 1164
AfaI GTAC 3 cut(s) 554, 608, 1127
AfiI CCNNNNNNNGG 2 cut(s) 241, 1248
AflIII ACRYGT 2 cut(s) 402, 1092
AgsI TTSAA 6 cut(s) 74, 378, 484, 547, 715, 958
AhlI ACTAGT 1 cut(s) 391
AjiI CACGTC 1 cut(s) 1095
AluBI AGCT 9 cut(s) 7, 139, 349, 848, 1004, 1032, 1179, 1314, 1338
AluI AGCT 9 cut(s) 7, 139, 349, 848, 1004, 1032, 1179, 1314, 1338
Alw21I GWGCWC 1 cut(s) 9
AlwI GGATC 2 cut(s) 449, 1325
AlwNI CAGNNNCTG 1 cut(s) 1004
Ama87I CYCGRG 3 cut(s) 229, 490, 1055
AoxI GGCC 3 cut(s) 120, 232, 519
ApeKI GCWGC 6 cut(s) 67, 276, 768, 839, 1004, 1075
ApoI RAATTY 2 cut(s) 366, 1164
ArsI GACNNNNNNTTYG 4 cut(s) 141, 173, 314, 346
Asp700I GAANNNNTTC 3 cut(s) 542, 903, 1295
Asp718I GGTACC 1 cut(s) 606
AspLEI GCGC 1 cut(s) 1075
AspS9I GGNCC 2 cut(s) 232, 254
AsuHPI GGTGA 3 cut(s) 363, 1169, 1186
AsuII TTCGAA 1 cut(s) 865
AvaI CYCGRG 3 cut(s) 229, 490, 1055
AvaII GGWCC 1 cut(s) 254
BalI TGGCCA 1 cut(s) 521
BanI GGYRCC 2 cut(s) 81, 606
BanII GRGCYC 1 cut(s) 9
BauI CACGAG 1 cut(s) 515
BbsI GAAGAC 4 cut(s) 288, 306, 470, 1089
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 6 cut(s) 54, 263, 755, 851, 991, 1062
BccI CCATC 4 cut(s) 227, 310, 490, 1373
BceAI ACGGC 3 cut(s) 450, 554, 912
BclI TGATCA 1 cut(s) 976
BcuI ACTAGT 1 cut(s) 391
BfaI CTAG 3 cut(s) 291, 392, 438
BfmI CTRYAG 1 cut(s) 1005
BfoI RGCGCY 1 cut(s) 1076
BisI GCNGC 8 cut(s) 68, 172, 277, 613, 769, 840, 1005, 1076
BlsI GCNGC 8 cut(s) 69, 173, 278, 614, 770, 841, 1006, 1077
Bme18I GGWCC 1 cut(s) 254
BmeT110I CYCGRG 3 cut(s) 229, 490, 1055
BmgBI CACGTC 1 cut(s) 1095
BmgT120I GGNCC 2 cut(s) 232, 254
BmiI GGNNCC 2 cut(s) 83, 608
BmsI GCATC 6 cut(s) 57, 621, 700, 709, 735, 748
BpiI GAAGAC 4 cut(s) 288, 306, 470, 1089
BpmI CTGGAG 1 cut(s) 1260
Bpu10I CCTNAGC 2 cut(s) 655, 1033
Bpu14I TTCGAA 1 cut(s) 865
BpuEI CTTGAG 2 cut(s) 680, 947
Bsa29I ATCGAT 1 cut(s) 703
BsaJI CCNNGG 3 cut(s) 235, 610, 802
BsaXI ACNNNNNCTCC 3 cut(s) 26, 1258, 1288
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 1248
Bse118I RCCGGY 1 cut(s) 272
Bse3DI GCAATG 2 cut(s) 24, 728
BseCI ATCGAT 1 cut(s) 703
BseDI CCNNGG 3 cut(s) 235, 610, 802
BseGI GGATG 2 cut(s) 165, 501
BseLI CCNNNNNNNGG 2 cut(s) 241, 1248
BseMI GCAATG 2 cut(s) 24, 728
BseMII CTCAG 2 cut(s) 669, 829
BseRI GAGGAG 3 cut(s) 200, 1156, 1367
BseXI GCAGC 6 cut(s) 54, 263, 755, 851, 991, 1062
BsgI GTGCAG 4 cut(s) 405, 523, 554, 1061
Bsh1236I CGCG 1 cut(s) 612
BshFI GGCC 3 cut(s) 122, 234, 521
BshNI GGYRCC 2 cut(s) 81, 606
BshVI ATCGAT 1 cut(s) 703
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 3 cut(s) 229, 490, 1055
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 1 cut(s) 1241
BslI CCNNNNNNNGG 2 cut(s) 241, 1248
BsmFI GGGAC 1 cut(s) 1241
BsmI GAATGC 1 cut(s) 1369
BsnI GGCC 3 cut(s) 122, 234, 521
BsoBI CYCGRG 3 cut(s) 229, 490, 1055
Bsp119I TTCGAA 1 cut(s) 865
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 8 cut(s) 162, 441, 526, 672, 704, 976, 1051, 1317
BspACI CCGC 7 cut(s) 133, 169, 172, 610, 612, 890, 1187
BspANI GGCC 3 cut(s) 122, 234, 521
BspCNI CTCAG 2 cut(s) 668, 828
BspDI ATCGAT 1 cut(s) 703
BspFNI CGCG 1 cut(s) 612
BspLI GGNNCC 2 cut(s) 83, 608
BspMAI CTGCAG 1 cut(s) 1009
BspPI GGATC 2 cut(s) 449, 1325
BspT104I TTCGAA 1 cut(s) 865
BspT107I GGYRCC 2 cut(s) 81, 606
BsrBI CCGCTC 2 cut(s) 133, 1187
BsrDI GCAATG 2 cut(s) 24, 728
BsrFI RCCGGY 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 3 cut(s) 235, 610, 802
BssMI GATC 8 cut(s) 162, 441, 526, 672, 704, 976, 1051, 1317
BssSI CACGAG 1 cut(s) 515
BssT1I CCWWGG 1 cut(s) 235
Bst2BI CACGAG 1 cut(s) 515
Bst4CI ACNGT 4 cut(s) 254, 287, 309, 452
BstBI TTCGAA 1 cut(s) 865
BstC8I GCNNGC 7 cut(s) 46, 274, 338, 594, 636, 952, 1189
BstDEI CTNAG 4 cut(s) 655, 815, 1033, 1137
BstDSI CCRYGG 2 cut(s) 610, 802
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 2 cut(s) 165, 501
BstFNI CGCG 1 cut(s) 612
BstH2I RGCGCY 1 cut(s) 1076
BstHHI GCGC 1 cut(s) 1075
BstKTI GATC 8 cut(s) 165, 444, 529, 675, 707, 979, 1054, 1320
BstMBI GATC 8 cut(s) 162, 441, 526, 672, 704, 976, 1051, 1317
BstMWI GCNNNNNNNGC 9 cut(s) 346, 510, 631, 651, 697, 836, 845, 1072, 1197
BstSFI CTRYAG 1 cut(s) 1005
BstUI CGCG 1 cut(s) 612
BstV1I GCAGC 6 cut(s) 54, 263, 755, 851, 991, 1062
BstV2I GAAGAC 4 cut(s) 288, 306, 470, 1089
Bsu15I ATCGAT 1 cut(s) 703
BsuRI GGCC 3 cut(s) 122, 234, 521
BsuTUI ATCGAT 1 cut(s) 703
BtgI CCRYGG 2 cut(s) 610, 802
BtgZI GCGATG 1 cut(s) 253
BtrI CACGTC 1 cut(s) 1095
BtsCI GGATG 2 cut(s) 165, 501
BtsI GCAGTG 1 cut(s) 511
BtsIMutI CAGTG 2 cut(s) 305, 511
Cac8I GCNNGC 7 cut(s) 46, 274, 338, 594, 636, 952, 1189
CaiI CAGNNNCTG 1 cut(s) 1004
CfoI GCGC 1 cut(s) 1075
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 2 cut(s) 232, 254
Cfr42I CCGCGG 1 cut(s) 613
ClaI ATCGAT 1 cut(s) 703
CseI GACGC 1 cut(s) 145
Csp6I GTAC 3 cut(s) 553, 607, 1126
CviAII CATG 6 cut(s) 628, 775, 856, 1129, 1231, 1300
CviQI GTAC 3 cut(s) 553, 607, 1126
DdeI CTNAG 4 cut(s) 655, 815, 1033, 1137
DpnI GATC 8 cut(s) 164, 443, 528, 674, 706, 978, 1053, 1319
DpnII GATC 8 cut(s) 162, 441, 526, 672, 704, 976, 1051, 1317
EaeI YGGCCR 1 cut(s) 519
EciI GGCGGA 1 cut(s) 158
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 9
Eco47I GGWCC 1 cut(s) 254
Eco53kI GAGCTC 1 cut(s) 7
Eco88I CYCGRG 3 cut(s) 229, 490, 1055
EcoICRI GAGCTC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 235
EcoT22I ATGCAT 2 cut(s) 693, 728
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 6 cut(s) 631, 778, 859, 1132, 1234, 1303
FaqI GGGAC 1 cut(s) 1241
FatI CATG 6 cut(s) 627, 774, 855, 1128, 1230, 1299
FauI CCCGC 1 cut(s) 1180
FbaI TGATCA 1 cut(s) 976
Fnu4HI GCNGC 8 cut(s) 68, 172, 277, 613, 769, 840, 1005, 1076
FokI GGATG 2 cut(s) 152, 508
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 8 cut(s) 68, 172, 277, 613, 769, 840, 1005, 1076
FspBI CTAG 3 cut(s) 291, 392, 438
GlaI GCGC 1 cut(s) 1074
GluI GCNGC 8 cut(s) 68, 172, 277, 613, 769, 840, 1005, 1076
GsuI CTGGAG 1 cut(s) 1260
HaeII RGCGCY 1 cut(s) 1076
HaeIII GGCC 3 cut(s) 122, 234, 521
HapII CCGG 1 cut(s) 273
HgaI GACGC 1 cut(s) 145
HhaI GCGC 1 cut(s) 1075
Hin1II CATG 6 cut(s) 631, 778, 859, 1132, 1234, 1303
Hin6I GCGC 1 cut(s) 1073
HinP1I GCGC 1 cut(s) 1073
HincII GTYRAC 3 cut(s) 226, 400, 1385
HindII GTYRAC 3 cut(s) 226, 400, 1385
HinfI GANTC 8 cut(s) 149, 302, 587, 798, 867, 958, 1038, 1291
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 1 cut(s) 273
HphI GGTGA 3 cut(s) 363, 1169, 1186
Hpy166II GTNNAC 4 cut(s) 226, 400, 749, 1385
Hpy188I TCNGA 2 cut(s) 600, 1346
Hpy188III TCNNGA 6 cut(s) 160, 547, 657, 1055, 1277, 1394
Hpy8I GTNNAC 4 cut(s) 226, 400, 749, 1385
HpyAV CCTTC 4 cut(s) 384, 435, 752, 872
HpyCH4III ACNGT 4 cut(s) 254, 287, 309, 452
HpyCH4IV ACGT 3 cut(s) 402, 551, 1094
HpyF10VI GCNNNNNNNGC 9 cut(s) 346, 510, 631, 651, 697, 836, 845, 1072, 1197
HpyF3I CTNAG 4 cut(s) 655, 815, 1033, 1137
HpySE526I ACGT 3 cut(s) 402, 551, 1094
Hsp92II CATG 6 cut(s) 631, 778, 859, 1132, 1234, 1303
HspAI GCGC 1 cut(s) 1073
KpnI GGTACC 1 cut(s) 610
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 976
KspAI GTTAAC 1 cut(s) 226
KspI CCGCGG 1 cut(s) 613
Kzo9I GATC 8 cut(s) 162, 441, 526, 672, 704, 976, 1051, 1317
LmnI GCTCC 4 cut(s) 4, 601, 776, 1184
Lsp1109I GCAGC 6 cut(s) 54, 263, 755, 851, 991, 1062
LweI GCATC 6 cut(s) 57, 621, 700, 709, 735, 748
MaeI CTAG 3 cut(s) 291, 392, 438
MaeII ACGT 3 cut(s) 402, 551, 1094
MaeIII GTNAC 2 cut(s) 452, 885
MalI GATC 8 cut(s) 164, 443, 528, 674, 706, 978, 1053, 1319
MbiI CCGCTC 2 cut(s) 133, 1187
MboI GATC 8 cut(s) 162, 441, 526, 672, 704, 976, 1051, 1317
MboII GAAGA 6 cut(s) 206, 293, 311, 475, 797, 1089
MfeI CAATTG 1 cut(s) 1204
MhlI GDGCHC 1 cut(s) 9
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 8 cut(s) 366, 381, 601, 716, 899, 1164, 1204, 1368
MluNI TGGCCA 1 cut(s) 521
MlyI GAGTC 2 cut(s) 296, 1032
MmeI TCCRAC 1 cut(s) 1003
Mox20I TGGCCA 1 cut(s) 521
Mph1103I ATGCAT 2 cut(s) 693, 728
MroNI GCCGGC 1 cut(s) 272
MroXI GAANNNNTTC 3 cut(s) 542, 903, 1295
MscI TGGCCA 1 cut(s) 521
MseI TTAA 3 cut(s) 225, 810, 1149
MslI CAYNNNNRTG 1 cut(s) 392
Msp20I TGGCCA 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 612
MspI CCGG 1 cut(s) 273
MunI CAATTG 1 cut(s) 1204
Mva1269I GAATGC 1 cut(s) 1369
MvnI CGCG 1 cut(s) 612
MwoI GCNNNNNNNGC 9 cut(s) 346, 510, 631, 651, 697, 836, 845, 1072, 1197
NaeI GCCGGC 1 cut(s) 274
NdeII GATC 8 cut(s) 162, 441, 526, 672, 704, 976, 1051, 1317
NgoMIV GCCGGC 1 cut(s) 272
NlaIII CATG 6 cut(s) 631, 778, 859, 1132, 1234, 1303
NlaIV GGNNCC 2 cut(s) 83, 608
NsiI ATGCAT 2 cut(s) 693, 728
NspV TTCGAA 1 cut(s) 865
PaeR7I CTCGAG 1 cut(s) 1055
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 596
PctI GAATGC 1 cut(s) 1369
PdiI GCCGGC 1 cut(s) 274
PdmI GAANNNNTTC 3 cut(s) 542, 903, 1295
PfeI GAWTC 6 cut(s) 149, 587, 798, 867, 958, 1291
PflMI CCANNNNNTGG 1 cut(s) 1248
PkrI GCNGC 8 cut(s) 69, 173, 278, 614, 770, 841, 1006, 1077
PleI GAGTC 2 cut(s) 296, 1032
PpsI GAGTC 2 cut(s) 296, 1032
Psp124BI GAGCTC 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 83, 608
PspPI GGNCC 2 cut(s) 232, 254
PstI CTGCAG 1 cut(s) 1009
PstNI CAGNNNCTG 1 cut(s) 1004
RsaI GTAC 3 cut(s) 554, 608, 1127
RsaNI GTAC 3 cut(s) 553, 607, 1126
RseI CAYNNNNRTG 1 cut(s) 392
SacI GAGCTC 1 cut(s) 9
SacII CCGCGG 1 cut(s) 613
SaqAI TTAA 3 cut(s) 225, 810, 1149
SatI GCNGC 8 cut(s) 68, 172, 277, 613, 769, 840, 1005, 1076
Sau3AI GATC 8 cut(s) 162, 441, 526, 672, 704, 976, 1051, 1317
Sau96I GGNCC 2 cut(s) 232, 254
SchI GAGTC 2 cut(s) 296, 1032
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 6 cut(s) 57, 621, 700, 709, 735, 748
SfcI CTRYAG 1 cut(s) 1005
Sfr274I CTCGAG 1 cut(s) 1055
Sfr303I CCGCGG 1 cut(s) 613
SfuI TTCGAA 1 cut(s) 865
SgrBI CCGCGG 1 cut(s) 613
SinI GGWCC 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 1055
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 3 cut(s) 695, 962, 1055
SmoI CTYRAG 3 cut(s) 695, 962, 1055
SpeI ACTAGT 1 cut(s) 391
Sse9I AATT 8 cut(s) 366, 381, 601, 716, 899, 1164, 1204, 1368
SsiI CCGC 7 cut(s) 133, 169, 172, 610, 612, 890, 1187
SspI AATATT 1 cut(s) 1331
SspMI CTAG 3 cut(s) 291, 392, 438
SstI GAGCTC 1 cut(s) 9
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 4 cut(s) 254, 287, 309, 452
TaiI ACGT 3 cut(s) 405, 554, 1097
TaqI TCGA 4 cut(s) 703, 753, 865, 1056
TasI AATT 8 cut(s) 366, 381, 601, 716, 899, 1164, 1204, 1368
TauI GCSGC 2 cut(s) 174, 615
TfiI GAWTC 6 cut(s) 149, 587, 798, 867, 958, 1291
Tru1I TTAA 3 cut(s) 225, 810, 1149
Tru9I TTAA 3 cut(s) 225, 810, 1149
TscAI CASTG 2 cut(s) 312, 511
TseI GCWGC 6 cut(s) 67, 276, 768, 839, 1004, 1075
TspDTI ATGAA 7 cut(s) 476, 771, 804, 872, 1288, 1341, 1376
TspGWI ACGGA 2 cut(s) 482, 791
TspRI CASTG 2 cut(s) 312, 511
Van91I CCANNNNNTGG 1 cut(s) 1248
VpaK11BI GGWCC 1 cut(s) 254
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 2 cut(s) 366, 1164
XhoI CTCGAG 1 cut(s) 1055
XmnI GAANNNNTTC 3 cut(s) 542, 903, 1295
XspI CTAG 3 cut(s) 291, 392, 438
Zsp2I ATGCAT 2 cut(s) 693, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.