Prupe.2G226000_v2.0.a1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0016938
Physical Location & Seq
Reverse (-)
25269543 .. 25271598
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G226000.1

Sequence Viewer

Length: 1425 bp
ATGGAGCTTTTCATGGCAATGTGTTTATTGCCTCTGTTCTGTGCTTGTCTCTACCTCTGTAAGTTGGTTCTTCAATGGAGGAACCAACAATGCTACTTGCTGGCCTATGAGTGCTACAAGGCCACCGATGACAGGAAACTCGACACTGAGTCCTGTGTCAAAATCATTCAGCGGAACAAGAACCTTGGCCTAGAAGAATACAGGTTTCTCCTCAAGACTATTGTCAATTCGGGCCTCGGGGAAGAAACTTACGGTCCAAGAAATGTCATTGCTGGCCGCGAAGATTGCCCTAGCTTGGAAGATGCACTGTGGGAGATGGATGATATATTCTCTGTCACACTTGACAAGCTGTTTGACAGAACAGGAATTTCACCTTCGAAAATCGACATACTTGTTGTCAATGTGTCACTCTTCTCCCCTGCGCCTTCTCTAACGTCTAGGATTATAAACCATTACAAGATGAAGAAGGATATCAAGGCCTTCAACCTCTCTGGCATGGGCTGCAGTGGGAGCCTTGTGGCCATTGATCTTGTGCAGAACTTGTTCAAGACATATAAGAATGTACATGCTGTTGTTGTGAGCACAGAGTCCCTTGGTCCAAATTGGTACCGCGGCAAAGTGAAGCCTATGATTCTTGCTAACTGTCTTTACCGGTCAGGAGGGTGTTCTATGCTTTTCACAAACAACAAGGCTCTCAAGCACAGAGCCCTCTTGAAATTGGAATGCATTGTAAGAACCCATCATGGTTCGAGTGATGAAGGCTATGGCTGCTCCATACAAGTAGAAGATAAACATGGGTTCAGGGGTTTTAATCTCAGCAAAAGCATAGCCAAAGTTGCTGCCAAAGCTCTTACTATGAACCTGCGTGTGCTGGTGCGCAAAGTGCTGCCGCTGACTGAATTGCTGCGCTATGTGATCATGTCTCGCCGCCAAAACAAAGTTGCTGAGCATCGCCGCGGTGTTGGAGCAGACGGGACAGGTTTGAATCTCAAGGCTGGACTTGAGCACTTCTGCATACCGCCTACAGAAGGTGCAGTTGTTAATGGGGTTGGCAATAGTTTAGGCCTCAATGACTATGATCTAGAGCCTGCTCGAATGGCACTTCACCGATTTGGCAACACATCCTCCGGTGGCCTTTGGTATGTTTTGGGATACATGGAGGCCAAGAAGCGGCTTAAGAAGCGAGACAAGATTCTGATGGTGAGCTTTGGTGCAGGTTATGAGTGCAATAATTGTGTGTGGGTGGTGTTGAGGGATTTGCATGATGCCAATGTGTGGAAAGACTGCATAGAAAGGTACCCTCCAACAACTTTGGTCAACCCTTTCATGGATAGGCTTAGTTGGATCAATGATGAACGCCTAAGCTTTGTCACATTTGAGGAGGTTATGAATGCAGTGCGTGTAGGTGGCGTTGGACCTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

475

Amino Acids

53.24

Weight (kDa)

8.95

Isoelectric Point (pI)

33.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016938)

Species Orthologous Gene IDs
malus_domestica MD01G1119300.v1.1 MD07G1188100.v1.1
prunus_persica Prupe.2G226000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365261
rosa_laevigata RLG00000027436
rosa_multiflora Rmu_sc0020532.1_g000001
rosa_roxburghii Rroxscaffold_4G00291340 Rroxscaffold_4G00291880
rosa_rugosa Rorug01G0320500
rosa_samantha Rh1AG329500 Rh1BG290900 Rh1CG307800 Rh1DG322600
rosa_wichuraiana Rw1G029220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
Acc16I TGCGCA 1 cut(s) 878
Acc36I ACCTGC 2 cut(s) 870, 1205
Acc65I GGTACC 2 cut(s) 606, 1296
AccB1I GGYRCC 2 cut(s) 606, 1296
AccB7I CCANNNNNTGG 1 cut(s) 1275
AccII CGCG 3 cut(s) 279, 612, 957
AclWI GGATC 1 cut(s) 1352
AcoI YGGCCR 2 cut(s) 274, 519
AcsI RAATTY 1 cut(s) 366
AfaI GTAC 3 cut(s) 564, 608, 1298
AfiI CCNNNNNNNGG 6 cut(s) 132, 295, 1028, 1170, 1275, 1327
AflII CTTAAG 1 cut(s) 1175
AgeI ACCGGT 1 cut(s) 651
AgsI TTSAA 5 cut(s) 74, 484, 547, 715, 985
AhdI GACNNNNNGTC 1 cut(s) 148
AjnI CCWGG 1 cut(s) 1417
AluBI AGCT 6 cut(s) 7, 294, 349, 848, 1206, 1365
AluI AGCT 6 cut(s) 7, 294, 349, 848, 1206, 1365
Alw21I GWGCWC 2 cut(s) 584, 1008
Alw26I GTCTC 3 cut(s) 53, 927, 1179
AlwI GGATC 1 cut(s) 1352
Ama87I CYCGRG 1 cut(s) 236
ApeKI GCWGC 5 cut(s) 501, 768, 839, 886, 904
ApoI RAATTY 1 cut(s) 366
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AsiGI ACCGGT 1 cut(s) 651
Asp700I GAANNNNTTC 1 cut(s) 542
Asp718I GGTACC 2 cut(s) 606, 1296
AspLEI GCGC 3 cut(s) 424, 879, 909
AspS9I GGNCC 4 cut(s) 232, 254, 596, 1415
AsuHPI GGTGA 3 cut(s) 363, 1097, 1213
AsuII TTCGAA 1 cut(s) 377
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 3 cut(s) 254, 596, 1415
BalI TGGCCA 1 cut(s) 521
BanI GGYRCC 2 cut(s) 606, 1296
BanII GRGCYC 1 cut(s) 709
Bbv12I GWGCWC 2 cut(s) 584, 1008
BbvI GCAGC 5 cut(s) 488, 755, 826, 873, 891
BccI CCATC 3 cut(s) 310, 747, 1192
BciT130I CCWGG 1 cut(s) 1419
BciVI GTATCC 1 cut(s) 1145
BclI TGATCA 1 cut(s) 915
BcoDI GTCTC 3 cut(s) 53, 927, 1179
BfaI CTAG 4 cut(s) 191, 291, 438, 1082
BfmI CTRYAG 2 cut(s) 502, 1023
BfrI CTTAAG 1 cut(s) 1175
BfuAI ACCTGC 2 cut(s) 870, 1205
BfuI GTATCC 1 cut(s) 1145
BlpI GCTNAGC 1 cut(s) 945
Bme1390I CCNGG 1 cut(s) 1419
Bme18I GGWCC 3 cut(s) 254, 596, 1415
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 4 cut(s) 232, 254, 596, 1415
BmiI GGNNCC 4 cut(s) 83, 512, 608, 1298
BmrFI CCNGG 1 cut(s) 1419
BmsI GCATC 3 cut(s) 292, 958, 1255
BoxI GACNNNNGTC 1 cut(s) 221
Bpu10I CCTNAGC 1 cut(s) 1361
Bpu1102I GCTNAGC 1 cut(s) 945
Bpu14I TTCGAA 1 cut(s) 377
BpuEI CTTGAG 4 cut(s) 197, 680, 974, 1022
BsaJI CCNNGG 5 cut(s) 184, 235, 592, 610, 955
BsaWI WCCGGW 2 cut(s) 651, 1127
BsaXI ACNNNNNCTCC 2 cut(s) 1109, 1139
Bsc4I CCNNNNNNNGG 6 cut(s) 132, 295, 1028, 1170, 1275, 1327
Bse118I RCCGGY 1 cut(s) 651
Bse3DI GCAATG 2 cut(s) 24, 267
BseBI CCWGG 1 cut(s) 1419
BseDI CCNNGG 5 cut(s) 184, 235, 592, 610, 955
BseGI GGATG 2 cut(s) 325, 1121
BseLI CCNNNNNNNGG 6 cut(s) 132, 295, 1028, 1170, 1275, 1327
BseMI GCAATG 2 cut(s) 24, 267
BseMII CTCAG 3 cut(s) 138, 829, 936
BseRI GAGGAG 2 cut(s) 200, 1394
BseXI GCAGC 5 cut(s) 488, 755, 826, 873, 891
BsgI GTGCAG 3 cut(s) 554, 1053, 1233
Bsh1236I CGCG 3 cut(s) 279, 612, 957
BshNI GGYRCC 2 cut(s) 606, 1296
BshTI ACCGGT 1 cut(s) 651
BsiHKAI GWGCWC 2 cut(s) 584, 1008
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 2 cut(s) 652, 1128
BslFI GGGAC 2 cut(s) 574, 988
BslI CCNNNNNNNGG 6 cut(s) 132, 295, 1028, 1170, 1275, 1327
BsmAI GTCTC 3 cut(s) 53, 927, 1179
BsmFI GGGAC 2 cut(s) 574, 988
BsmI GAATGC 2 cut(s) 728, 1396
BsoBI CYCGRG 1 cut(s) 236
Bsp119I TTCGAA 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 584, 709, 1008
Bsp1407I TGTACA 1 cut(s) 562
Bsp143I GATC 4 cut(s) 526, 915, 1078, 1344
Bsp1720I GCTNAGC 1 cut(s) 945
BspCNI CTCAG 3 cut(s) 139, 828, 937
BspFNI CGCG 3 cut(s) 279, 612, 957
BspLI GGNNCC 4 cut(s) 83, 512, 608, 1298
BspMAI CTGCAG 1 cut(s) 506
BspMI ACCTGC 2 cut(s) 870, 1205
BspPI GGATC 1 cut(s) 1352
BspT104I TTCGAA 1 cut(s) 377
BspT107I GGYRCC 2 cut(s) 606, 1296
BspTI CTTAAG 1 cut(s) 1175
BsrDI GCAATG 2 cut(s) 24, 267
BsrFI RCCGGY 1 cut(s) 651
BsrGI TGTACA 1 cut(s) 562
BssAI RCCGGY 1 cut(s) 651
BssECI CCNNGG 5 cut(s) 184, 235, 592, 610, 955
BssMI GATC 4 cut(s) 526, 915, 1078, 1344
BssT1I CCWWGG 2 cut(s) 184, 592
Bst2UI CCWGG 1 cut(s) 1419
Bst4CI ACNGT 3 cut(s) 254, 309, 644
Bst6I CTCTTC 1 cut(s) 416
BstAFI CTTAAG 1 cut(s) 1175
BstAPI GCANNNNNTGC 1 cut(s) 501
BstAUI TGTACA 1 cut(s) 562
BstBI TTCGAA 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 102, 274, 1089
BstDEI CTNAG 5 cut(s) 147, 815, 945, 1337, 1361
BstDSI CCRYGG 2 cut(s) 610, 955
BstENI CCTNNNNNAGG 1 cut(s) 1026
BstF5I GGATG 2 cut(s) 325, 1121
BstFNI CGCG 3 cut(s) 279, 612, 957
BstHHI GCGC 3 cut(s) 424, 879, 909
BstKTI GATC 4 cut(s) 529, 918, 1081, 1347
BstMAI GTCTC 3 cut(s) 53, 927, 1179
BstMBI GATC 4 cut(s) 526, 915, 1078, 1344
BstMWI GCNNNNNNNGC 9 cut(s) 285, 501, 510, 768, 836, 845, 883, 1097, 1180
BstNI CCWGG 1 cut(s) 1419
BstNSI RCATGY 1 cut(s) 569
BstPAI GACNNNNGTC 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 1417
BstSFI CTRYAG 2 cut(s) 502, 1023
BstUI CGCG 3 cut(s) 279, 612, 957
BstV1I GCAGC 5 cut(s) 488, 755, 826, 873, 891
BsuI GTATCC 1 cut(s) 1145
BtgI CCRYGG 2 cut(s) 610, 955
BtgZI GCGATG 1 cut(s) 935
BtsCI GGATG 2 cut(s) 325, 1121
BtsI GCAGTG 2 cut(s) 511, 1401
BtsIMutI CAGTG 4 cut(s) 144, 305, 511, 1401
BveI ACCTGC 2 cut(s) 870, 1205
Cac8I GCNNGC 3 cut(s) 102, 274, 1089
CfoI GCGC 3 cut(s) 424, 879, 909
Cfr10I RCCGGY 1 cut(s) 651
Cfr13I GGNCC 4 cut(s) 232, 254, 596, 1415
Cfr42I CCGCGG 2 cut(s) 613, 958
Csp6I GTAC 3 cut(s) 563, 607, 1297
CspAI ACCGGT 1 cut(s) 651
CviAII CATG 9 cut(s) 13, 496, 566, 743, 794, 919, 1156, 1262, 1327
CviQI GTAC 3 cut(s) 563, 607, 1297
DdeI CTNAG 5 cut(s) 147, 815, 945, 1337, 1361
DpnI GATC 4 cut(s) 528, 917, 1080, 1346
DpnII GATC 4 cut(s) 526, 915, 1078, 1344
DriI GACNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 2 cut(s) 274, 519
Eam1104I CTCTTC 1 cut(s) 416
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 184, 592
Eco147I AGGCCT 2 cut(s) 479, 1065
Eco24I GRGCYC 1 cut(s) 709
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 3 cut(s) 254, 596, 1415
Eco88I CYCGRG 1 cut(s) 236
EcoNI CCTNNNNNAGG 1 cut(s) 1026
EcoRII CCWGG 1 cut(s) 1417
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 2 cut(s) 184, 592
EcoT22I ATGCAT 1 cut(s) 728
EcoT38I GRGCYC 1 cut(s) 709
ErhI CCWWGG 2 cut(s) 184, 592
FaeI CATG 9 cut(s) 16, 499, 569, 746, 797, 922, 1159, 1265, 1330
FaqI GGGAC 2 cut(s) 574, 988
FatI CATG 9 cut(s) 12, 495, 565, 742, 793, 918, 1155, 1261, 1326
FbaI TGATCA 1 cut(s) 915
FokI GGATG 2 cut(s) 332, 1108
FriOI GRGCYC 1 cut(s) 709
FspBI CTAG 4 cut(s) 191, 291, 438, 1082
FspI TGCGCA 1 cut(s) 878
GlaI GCGC 3 cut(s) 423, 878, 908
HapII CCGG 2 cut(s) 652, 1128
HhaI GCGC 3 cut(s) 424, 879, 909
Hin1II CATG 9 cut(s) 16, 499, 569, 746, 797, 922, 1159, 1265, 1330
Hin6I GCGC 3 cut(s) 422, 877, 907
HinP1I GCGC 3 cut(s) 422, 877, 907
HincII GTYRAC 1 cut(s) 1318
HindII GTYRAC 1 cut(s) 1318
HindIII AAGCTT 1 cut(s) 1363
HinfI GANTC 5 cut(s) 149, 587, 631, 985, 1192
HpaII CCGG 2 cut(s) 652, 1128
HphI GGTGA 3 cut(s) 363, 1097, 1213
Hpy166II GTNNAC 1 cut(s) 1318
Hpy188I TCNGA 1 cut(s) 1197
Hpy188III TCNNGA 5 cut(s) 214, 547, 657, 712, 1082
Hpy8I GTNNAC 1 cut(s) 1318
HpyAV CCTTC 6 cut(s) 384, 435, 460, 490, 752, 1022
HpyCH4III ACNGT 3 cut(s) 254, 309, 644
HpyCH4IV ACGT 1 cut(s) 434
HpyF10VI GCNNNNNNNGC 9 cut(s) 285, 501, 510, 768, 836, 845, 883, 1097, 1180
HpyF3I CTNAG 5 cut(s) 147, 815, 945, 1337, 1361
HpySE526I ACGT 1 cut(s) 434
Hsp92II CATG 9 cut(s) 16, 499, 569, 746, 797, 922, 1159, 1265, 1330
HspAI GCGC 3 cut(s) 422, 877, 907
KpnI GGTACC 2 cut(s) 610, 1300
Ksp22I TGATCA 1 cut(s) 915
KspI CCGCGG 2 cut(s) 613, 958
Kzo9I GATC 4 cut(s) 526, 915, 1078, 1344
LmnI GCTCC 4 cut(s) 4, 510, 776, 965
Lsp1109I GCAGC 5 cut(s) 488, 755, 826, 873, 891
LweI GCATC 3 cut(s) 292, 958, 1255
MaeI CTAG 4 cut(s) 191, 291, 438, 1082
MaeII ACGT 1 cut(s) 434
MaeIII GTNAC 3 cut(s) 334, 405, 1369
MalI GATC 4 cut(s) 528, 917, 1080, 1346
MboI GATC 4 cut(s) 526, 915, 1078, 1344
MboII GAAGA 8 cut(s) 62, 206, 254, 293, 311, 403, 475, 797
MhlI GDGCHC 3 cut(s) 584, 709, 1008
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 6 cut(s) 226, 366, 601, 716, 899, 1231
MluNI TGGCCA 1 cut(s) 521
MlyI GAGTC 2 cut(s) 158, 596
MmeI TCCRAC 4 cut(s) 943, 1322, 1328, 1393
Mox20I TGGCCA 1 cut(s) 521
Mph1103I ATGCAT 1 cut(s) 728
MroXI GAANNNNTTC 1 cut(s) 542
MscI TGGCCA 1 cut(s) 521
MseI TTAA 3 cut(s) 810, 1041, 1176
MslI CAYNNNNRTG 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 521
MspA1I CMGCKG 4 cut(s) 172, 612, 892, 957
MspCI CTTAAG 1 cut(s) 1175
MspI CCGG 2 cut(s) 652, 1128
MspR9I CCNGG 1 cut(s) 1419
Mva1269I GAATGC 2 cut(s) 728, 1396
MvaI CCWGG 1 cut(s) 1419
MvnI CGCG 3 cut(s) 279, 612, 957
MwoI GCNNNNNNNGC 9 cut(s) 285, 501, 510, 768, 836, 845, 883, 1097, 1180
NdeII GATC 4 cut(s) 526, 915, 1078, 1344
NlaIII CATG 9 cut(s) 16, 499, 569, 746, 797, 922, 1159, 1265, 1330
NlaIV GGNNCC 4 cut(s) 83, 512, 608, 1298
NmuCI GTSAC 3 cut(s) 334, 405, 1369
NsbI TGCGCA 1 cut(s) 878
NsiI ATGCAT 1 cut(s) 728
NspI RCATGY 1 cut(s) 569
NspV TTCGAA 1 cut(s) 377
PceI AGGCCT 2 cut(s) 479, 1065
PctI GAATGC 2 cut(s) 728, 1396
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 3 cut(s) 631, 985, 1192
PflMI CCANNNNNTGG 1 cut(s) 1275
PinAI ACCGGT 1 cut(s) 651
PleI GAGTC 2 cut(s) 157, 595
PpsI GAGTC 2 cut(s) 157, 595
PshAI GACNNNNGTC 1 cut(s) 221
PsiI TTATAA 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 1417
PspGI CCWGG 1 cut(s) 1417
PspN4I GGNNCC 4 cut(s) 83, 512, 608, 1298
PspPI GGNCC 4 cut(s) 232, 254, 596, 1415
PstI CTGCAG 1 cut(s) 506
RsaI GTAC 3 cut(s) 564, 608, 1298
RsaNI GTAC 3 cut(s) 563, 607, 1297
RseI CAYNNNNRTG 1 cut(s) 17
SacII CCGCGG 2 cut(s) 613, 958
SaqAI TTAA 3 cut(s) 810, 1041, 1176
Sau3AI GATC 4 cut(s) 526, 915, 1078, 1344
Sau96I GGNCC 4 cut(s) 232, 254, 596, 1415
SchI GAGTC 2 cut(s) 158, 596
ScrFI CCNGG 1 cut(s) 1419
SduI GDGCHC 3 cut(s) 584, 709, 1008
SfaNI GCATC 3 cut(s) 292, 958, 1255
SfcI CTRYAG 2 cut(s) 502, 1023
Sfr303I CCGCGG 2 cut(s) 613, 958
SfuI TTCGAA 1 cut(s) 377
SgrBI CCGCGG 2 cut(s) 613, 958
SinI GGWCC 3 cut(s) 254, 596, 1415
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 5 cut(s) 212, 695, 989, 1001, 1175
SmoI CTYRAG 5 cut(s) 212, 695, 989, 1001, 1175
Sse9I AATT 6 cut(s) 226, 366, 601, 716, 899, 1231
SseBI AGGCCT 2 cut(s) 479, 1065
SspMI CTAG 4 cut(s) 191, 291, 438, 1082
StuI AGGCCT 2 cut(s) 479, 1065
StyD4I CCNGG 1 cut(s) 1417
StyI CCWWGG 2 cut(s) 184, 592
TaaI ACNGT 3 cut(s) 254, 309, 644
TaiI ACGT 1 cut(s) 437
TaqI TCGA 5 cut(s) 141, 377, 384, 749, 1093
TasI AATT 6 cut(s) 226, 366, 601, 716, 899, 1231
TatI WGTACW 1 cut(s) 562
TauI GCSGC 6 cut(s) 279, 615, 892, 930, 957, 1174
TfiI GAWTC 3 cut(s) 631, 985, 1192
Tru1I TTAA 3 cut(s) 810, 1041, 1176
Tru9I TTAA 3 cut(s) 810, 1041, 1176
TscAI CASTG 4 cut(s) 151, 312, 511, 1401
TseFI GTSAC 3 cut(s) 334, 405, 1369
TseI GCWGC 5 cut(s) 501, 768, 839, 886, 904
Tsp45I GTSAC 3 cut(s) 334, 405, 1369
TspDTI ATGAA 6 cut(s) 476, 771, 872, 1315, 1368, 1403
TspRI CASTG 4 cut(s) 151, 312, 511, 1401
Van91I CCANNNNNTGG 1 cut(s) 1275
Vha464I CTTAAG 1 cut(s) 1175
VpaK11BI GGWCC 3 cut(s) 254, 596, 1415
XagI CCTNNNNNAGG 1 cut(s) 1026
XapI RAATTY 1 cut(s) 366
XbaI TCTAGA 1 cut(s) 1081
XceI RCATGY 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 4 cut(s) 191, 291, 438, 1082
Zsp2I ATGCAT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.