Rroxscaffold_4G00291880

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016938
Physical Location & Seq
Forward (+)
12233310 .. 12234716
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00291880.1

Sequence Viewer

Length: 1407 bp
ATGGAGCAGGTGATTTTCATGGCTTTGTGTTTACTTCCTCTATTGTATGCTTTTATCTACCTTTCTAAGCTGGTTCTTCAATGGAGAGACCAATGCTGCTACTTGTTGGCCTATGACTGCTACAAGGCCGCAGATGATAGGAAGCTCGACACCGATTCCTGCCTCAAAATCATTCTGCGCAACAAAAATCTTGGTCTAGAAGAGTACCGTTTTCTCTTAAAGACCATTGTCAATTCGGGTCTCGGAGAGGAAACATACAGTCCAAGGAATATCATGGCCGGCCGTGAAGACTGCCCTAGTTTGGGAGATGCACTATTGGAAATGGATGACATTTTCTTCACCACGCTCGACAATCTTTTTAATAGAAGTGGAGTTTCACCCTCGGAGATTGACATACTTGTTGTGAATGTGTCATTGTTCTCTCCATCACCGTCTCTAACGGCTAGGATCATAAACCGGTACAAGATGAAGAGTAGCATCAAGGCCTTTAACCTCTCGGGAATGGGATGCAGTGGAAGCCTTGTTGCTATTGATCTTGTACAAAACATGTTCAAGACATACACGAAGATGTTTGCCGTTGTCGTGAGTACCGAATCTGTGGGTCCGAATTGGTACAGTGGCCAAGTCAAGCCGATGATGCTTGCTAATTGTCTATACCGGTCAGGAGGGTGTTCTATGCTTTTCACAAACAAGAGAGGTCTTAAGCATTTAGCCCTCTTGAAATTGAAATACATAGTCCGAACCCATCTTGGTTCTAGTGACGAAGGTTATGGCTGCTCAATTCAAGTGGAAGATAAGCATGGCTACAGAGGATTTAACCTCAGCAAAAGCATAGCAAAAGTTGCTGCTAAAGCTCTTACTATGAACCTGAGAGTGCTGGCACTTAAAGCGTTACCGATTACAGAATTATTCCGCTATGCGCTCAATTCTCGCTGTCGAAACAAAGCTGCTGCTAAAGAAGTGGTAAGTATGGGTTTGAATCTCAAGGCTGGACTTGACCATTTCTGCATACCCCCAACTGAAGCTGCAGTTGTTAATGGGATTGGGAAGAGATTGGCACTTAATGACTATGATTTAGAACCTTCGAGAATGGCGCTTCATCGGTTCGGCAACACATCCTCTGGTGGGCTTTGGTACGTTTTGGGTTACATGGAAGCTAAGAAGAGGCTTAAGAAAGGTAACAAGATTCTGATGGTGGGCTTTGGGGCCGGTTATGAATGCAATAACTGTGTATGGAAGGTGTTGAGGGATTTGGATGATGCCAATGTGTGGAAAGACTGCGTTGACAGATACCCTCCACCAAGCTTGATCAACCCTTTCATGCAAAGGTATAGTTGGCTTAACTATGAATTTTTGAGCTTTATTAGATTTGATCAAATTAGGAATGCAATGGATCAGTGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

468

Amino Acids

52.79

Weight (kDa)

9.1

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 27 - 309 1.7e-106 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 332 - 413 7.6e-09 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 336 - 405 2.5e-06 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016938)

Species Orthologous Gene IDs
malus_domestica MD01G1119300.v1.1 MD07G1188100.v1.1
prunus_persica Prupe.2G226000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365261
rosa_laevigata RLG00000027436
rosa_multiflora Rmu_sc0020532.1_g000001
rosa_roxburghii Rroxscaffold_4G00291340 Rroxscaffold_4G00291880
rosa_rugosa Rorug01G0320500
rosa_samantha Rh1AG329500 Rh1BG290900 Rh1CG307800 Rh1DG322600
rosa_wichuraiana Rw1G029220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 179
AccB7I CCANNNNNTGG 1 cut(s) 1269
AciI CCGC 2 cut(s) 129, 913
AclWI GGATC 2 cut(s) 455, 1401
AcoI YGGCCR 3 cut(s) 276, 280, 619
AcsI RAATTY 1 cut(s) 1349
AcuI CTGAAG 1 cut(s) 1041
AfaI GTAC 6 cut(s) 206, 461, 540, 589, 614, 1136
AfiI CCNNNNNNNGG 4 cut(s) 301, 302, 1125, 1269
AflII CTTAAG 2 cut(s) 701, 1169
AflIII ACRYGT 1 cut(s) 546
AgeI ACCGGT 2 cut(s) 456, 657
AgsI TTSAA 6 cut(s) 80, 553, 721, 727, 785, 979
AluBI AGCT 8 cut(s) 70, 145, 854, 947, 1025, 1157, 1305, 1359
AluI AGCT 8 cut(s) 70, 145, 854, 947, 1025, 1157, 1305, 1359
Alw26I GTCTC 3 cut(s) 81, 245, 438
AlwI GGATC 2 cut(s) 455, 1401
Ama87I CYCGRG 1 cut(s) 496
AoxI GGCC 7 cut(s) 108, 126, 276, 280, 483, 619, 1206
ApeKI GCWGC 6 cut(s) 96, 774, 845, 947, 950, 1025
ApoI RAATTY 1 cut(s) 1349
AsiGI ACCGGT 2 cut(s) 456, 657
AspLEI GCGC 3 cut(s) 180, 922, 1096
AspS9I GGNCC 2 cut(s) 602, 1206
AsuHPI GGTGA 4 cut(s) 22, 331, 369, 420
AvaI CYCGRG 1 cut(s) 496
AvaII GGWCC 1 cut(s) 602
BalI TGGCCA 1 cut(s) 621
BbsI GAAGAC 1 cut(s) 294
BbvCI CCTCAGC 1 cut(s) 821
BbvI GCAGC 6 cut(s) 83, 761, 832, 934, 937, 1012
BccI CCATC 3 cut(s) 433, 753, 1186
BceAI ACGGC 3 cut(s) 267, 456, 560
BclI TGATCA 2 cut(s) 1308, 1372
BcoDI GTCTC 3 cut(s) 81, 245, 438
BfaI CTAG 4 cut(s) 197, 297, 444, 756
BfmI CTRYAG 2 cut(s) 805, 1026
BfoI RGCGCY 1 cut(s) 1097
BfrI CTTAAG 2 cut(s) 701, 1169
BisI GCNGC 7 cut(s) 97, 129, 775, 846, 948, 951, 1026
BlsI GCNGC 7 cut(s) 98, 130, 776, 847, 949, 952, 1027
Bme18I GGWCC 1 cut(s) 602
BmeT110I CYCGRG 1 cut(s) 496
BmgT120I GGNCC 2 cut(s) 602, 1206
BmiI GGNNCC 2 cut(s) 603, 1207
BmsI GCATC 5 cut(s) 298, 486, 497, 627, 1249
BoxI GACNNNNGTC 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 821
BpuEI CTTGAG 1 cut(s) 968
BsaI GGTCTC 2 cut(s) 81, 245
BsaJI CCNNGG 2 cut(s) 263, 381
BsaWI WCCGGW 2 cut(s) 456, 657
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 302, 1125, 1269
Bse118I RCCGGY 4 cut(s) 278, 456, 657, 1208
Bse3DI GCAATG 1 cut(s) 1395
BseDI CCNNGG 2 cut(s) 263, 381
BseGI GGATG 4 cut(s) 331, 512, 1115, 1261
BseLI CCNNNNNNNGG 4 cut(s) 301, 302, 1125, 1269
BseMI GCAATG 1 cut(s) 1395
BseMII CTCAG 2 cut(s) 835, 860
BseX3I CGGCCG 1 cut(s) 280
BseXI GCAGC 6 cut(s) 83, 761, 832, 934, 937, 1012
Bsh1285I CGRYCG 1 cut(s) 283
BshFI GGCC 7 cut(s) 110, 128, 278, 282, 485, 621, 1208
BshTI ACCGGT 2 cut(s) 456, 657
BsiEI CGRYCG 1 cut(s) 283
BsiHKCI CYCGRG 1 cut(s) 496
BsiSI CCGG 4 cut(s) 279, 457, 658, 1209
BslI CCNNNNNNNGG 4 cut(s) 301, 302, 1125, 1269
BsmAI GTCTC 3 cut(s) 81, 245, 438
BsmBI CGTCTC 1 cut(s) 438
BsmI GAATGC 2 cut(s) 1223, 1390
BsnI GGCC 7 cut(s) 110, 128, 278, 282, 485, 621, 1208
Bso31I GGTCTC 2 cut(s) 81, 245
BsoBI CYCGRG 1 cut(s) 496
Bsp1407I TGTACA 1 cut(s) 538
Bsp143I GATC 5 cut(s) 447, 532, 1308, 1372, 1393
BspACI CCGC 2 cut(s) 129, 913
BspANI GGCC 7 cut(s) 110, 128, 278, 282, 485, 621, 1208
BspCNI CTCAG 2 cut(s) 834, 861
BspLI GGNNCC 2 cut(s) 603, 1207
BspMAI CTGCAG 1 cut(s) 1030
BspPI GGATC 2 cut(s) 455, 1401
BspTI CTTAAG 2 cut(s) 701, 1169
BspTNI GGTCTC 2 cut(s) 81, 245
BsrDI GCAATG 1 cut(s) 1395
BsrFI RCCGGY 4 cut(s) 278, 456, 657, 1208
BsrGI TGTACA 1 cut(s) 538
BssAI RCCGGY 4 cut(s) 278, 456, 657, 1208
BssECI CCNNGG 2 cut(s) 263, 381
BssMI GATC 5 cut(s) 447, 532, 1308, 1372, 1393
BssT1I CCWWGG 1 cut(s) 263
Bst4CI ACNGT 5 cut(s) 209, 260, 432, 617, 1229
Bst6I CTCTTC 4 cut(s) 195, 464, 1043, 1157
BstAFI CTTAAG 2 cut(s) 701, 1169
BstAPI GCANNNNNTGC 1 cut(s) 842
BstAUI TGTACA 1 cut(s) 538
BstC8I GCNNGC 3 cut(s) 280, 642, 879
BstDEI CTNAG 4 cut(s) 66, 821, 869, 1158
BstF5I GGATG 4 cut(s) 331, 512, 1115, 1261
BstH2I RGCGCY 1 cut(s) 1097
BstHHI GCGC 3 cut(s) 180, 922, 1096
BstKTI GATC 5 cut(s) 450, 535, 1311, 1375, 1396
BstMAI GTCTC 3 cut(s) 81, 245, 438
BstMBI GATC 5 cut(s) 447, 532, 1308, 1372, 1393
BstMCI CGRYCG 1 cut(s) 283
BstMWI GCNNNNNNNGC 5 cut(s) 516, 637, 842, 851, 887
BstNSI RCATGY 1 cut(s) 550
BstPAI GACNNNNGTC 1 cut(s) 227
BstSFI CTRYAG 2 cut(s) 805, 1026
BstV1I GCAGC 6 cut(s) 83, 761, 832, 934, 937, 1012
BstV2I GAAGAC 1 cut(s) 294
BstZI CGGCCG 1 cut(s) 280
BsuRI GGCC 7 cut(s) 110, 128, 278, 282, 485, 621, 1208
BtsCI GGATG 4 cut(s) 331, 512, 1115, 1261
BtsI GCAGTG 1 cut(s) 517
BtsIMutI CAGTG 3 cut(s) 517, 622, 1403
Cac8I GCNNGC 3 cut(s) 280, 642, 879
CfoI GCGC 3 cut(s) 180, 922, 1096
Cfr10I RCCGGY 4 cut(s) 278, 456, 657, 1208
Cfr13I GGNCC 2 cut(s) 602, 1206
Csp6I GTAC 6 cut(s) 205, 460, 539, 588, 613, 1135
CspAI ACCGGT 2 cut(s) 456, 657
CspCI CAANNNNNGTGG 4 cut(s) 768, 803, 1287, 1322
CviAII CATG 6 cut(s) 19, 274, 547, 800, 1150, 1321
CviQI GTAC 6 cut(s) 205, 460, 539, 588, 613, 1135
DdeI CTNAG 4 cut(s) 66, 821, 869, 1158
DpnI GATC 5 cut(s) 449, 534, 1310, 1374, 1395
DpnII GATC 5 cut(s) 447, 532, 1308, 1372, 1393
EaeI YGGCCR 3 cut(s) 276, 280, 619
EagI CGGCCG 1 cut(s) 280
Eam1104I CTCTTC 4 cut(s) 195, 464, 1043, 1157
EarI CTCTTC 4 cut(s) 195, 464, 1043, 1157
EclXI CGGCCG 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 263
Eco147I AGGCCT 1 cut(s) 485
Eco31I GGTCTC 2 cut(s) 81, 245
Eco47I GGWCC 1 cut(s) 602
Eco52I CGGCCG 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 1041
Eco88I CYCGRG 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 263
Esp3I CGTCTC 1 cut(s) 438
FaeI CATG 6 cut(s) 22, 277, 550, 803, 1153, 1324
FatI CATG 6 cut(s) 18, 273, 546, 799, 1149, 1320
FbaI TGATCA 2 cut(s) 1308, 1372
Fnu4HI GCNGC 7 cut(s) 97, 129, 775, 846, 948, 951, 1026
FokI GGATG 4 cut(s) 338, 519, 1102, 1268
FseI GGCCGGCC 1 cut(s) 282
Fsp4HI GCNGC 7 cut(s) 97, 129, 775, 846, 948, 951, 1026
FspBI CTAG 4 cut(s) 197, 297, 444, 756
FspI TGCGCA 1 cut(s) 179
GlaI GCGC 3 cut(s) 179, 921, 1095
GluI GCNGC 7 cut(s) 97, 129, 775, 846, 948, 951, 1026
HaeII RGCGCY 1 cut(s) 1097
HaeIII GGCC 7 cut(s) 110, 128, 278, 282, 485, 621, 1208
HapII CCGG 4 cut(s) 279, 457, 658, 1209
HhaI GCGC 3 cut(s) 180, 922, 1096
Hin1II CATG 6 cut(s) 22, 277, 550, 803, 1153, 1324
Hin6I GCGC 3 cut(s) 178, 920, 1094
HinP1I GCGC 3 cut(s) 178, 920, 1094
HincII GTYRAC 1 cut(s) 1285
HindII GTYRAC 1 cut(s) 1285
HindIII AAGCTT 1 cut(s) 1303
HinfI GANTC 4 cut(s) 155, 593, 979, 1186
HpaII CCGG 4 cut(s) 279, 457, 658, 1209
HphI GGTGA 4 cut(s) 22, 331, 369, 420
Hpy166II GTNNAC 2 cut(s) 32, 1285
Hpy188I TCNGA 5 cut(s) 245, 385, 606, 740, 1191
Hpy188III TCNNGA 7 cut(s) 197, 498, 553, 583, 663, 718, 1086
Hpy8I GTNNAC 2 cut(s) 32, 1285
HpyAV CCTTC 3 cut(s) 758, 1092, 1231
HpyCH4III ACNGT 5 cut(s) 209, 260, 432, 617, 1229
HpyCH4IV ACGT 1 cut(s) 1137
HpyCH4V TGCA 7 cut(s) 311, 510, 1008, 1028, 1221, 1324, 1388
HpyF10VI GCNNNNNNNGC 5 cut(s) 516, 637, 842, 851, 887
HpyF3I CTNAG 4 cut(s) 66, 821, 869, 1158
HpySE526I ACGT 1 cut(s) 1137
Hsp92II CATG 6 cut(s) 22, 277, 550, 803, 1153, 1324
HspAI GCGC 3 cut(s) 178, 920, 1094
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Ksp22I TGATCA 2 cut(s) 1308, 1372
Kzo9I GATC 5 cut(s) 447, 532, 1308, 1372, 1393
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 6 cut(s) 83, 761, 832, 934, 937, 1012
LweI GCATC 5 cut(s) 298, 486, 497, 627, 1249
MaeI CTAG 4 cut(s) 197, 297, 444, 756
MaeII ACGT 1 cut(s) 1137
MaeIII GTNAC 4 cut(s) 758, 891, 1145, 1178
MalI GATC 5 cut(s) 449, 534, 1310, 1374, 1395
MboI GATC 5 cut(s) 447, 532, 1308, 1372, 1393
MboII GAAGA 9 cut(s) 68, 212, 299, 328, 481, 577, 803, 1060, 1174
MlsI TGGCCA 1 cut(s) 621
MluCI AATT 9 cut(s) 232, 607, 646, 722, 780, 905, 925, 1349, 1377
MluNI TGGCCA 1 cut(s) 621
Mox20I TGGCCA 1 cut(s) 621
MroNI GCCGGC 1 cut(s) 278
MscI TGGCCA 1 cut(s) 621
MslI CAYNNNNRTG 1 cut(s) 566
Msp20I TGGCCA 1 cut(s) 621
MspCI CTTAAG 2 cut(s) 701, 1169
MspI CCGG 4 cut(s) 279, 457, 658, 1209
Mva1269I GAATGC 2 cut(s) 1223, 1390
MwoI GCNNNNNNNGC 5 cut(s) 516, 637, 842, 851, 887
NaeI GCCGGC 1 cut(s) 280
NdeII GATC 5 cut(s) 447, 532, 1308, 1372, 1393
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 6 cut(s) 22, 277, 550, 803, 1153, 1324
NlaIV GGNNCC 2 cut(s) 603, 1207
NmuCI GTSAC 1 cut(s) 758
NsbI TGCGCA 1 cut(s) 179
NspI RCATGY 1 cut(s) 550
PceI AGGCCT 1 cut(s) 485
PciI ACATGT 1 cut(s) 546
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PctI GAATGC 2 cut(s) 1223, 1390
PdiI GCCGGC 1 cut(s) 280
PfeI GAWTC 4 cut(s) 155, 593, 979, 1186
PflMI CCANNNNNTGG 1 cut(s) 1269
PinAI ACCGGT 2 cut(s) 456, 657
PkrI GCNGC 7 cut(s) 98, 130, 776, 847, 949, 952, 1027
PscI ACATGT 1 cut(s) 546
PshAI GACNNNNGTC 1 cut(s) 227
PspN4I GGNNCC 2 cut(s) 603, 1207
PspPI GGNCC 2 cut(s) 602, 1206
PstI CTGCAG 1 cut(s) 1030
RigI GGCCGGCC 1 cut(s) 282
RsaI GTAC 6 cut(s) 206, 461, 540, 589, 614, 1136
RsaNI GTAC 6 cut(s) 205, 460, 539, 588, 613, 1135
RseI CAYNNNNRTG 1 cut(s) 566
SatI GCNGC 7 cut(s) 97, 129, 775, 846, 948, 951, 1026
Sau3AI GATC 5 cut(s) 447, 532, 1308, 1372, 1393
Sau96I GGNCC 2 cut(s) 602, 1206
SfaNI GCATC 5 cut(s) 298, 486, 497, 627, 1249
SfcI CTRYAG 2 cut(s) 805, 1026
SinI GGWCC 1 cut(s) 602
SmiMI CAYNNNNRTG 1 cut(s) 566
SmlI CTYRAG 3 cut(s) 701, 983, 1169
SmoI CTYRAG 3 cut(s) 701, 983, 1169
Sse9I AATT 9 cut(s) 232, 607, 646, 722, 780, 905, 925, 1349, 1377
SseBI AGGCCT 1 cut(s) 485
SsiI CCGC 2 cut(s) 129, 913
SspMI CTAG 4 cut(s) 197, 297, 444, 756
StuI AGGCCT 1 cut(s) 485
StyI CCWWGG 1 cut(s) 263
TaaI ACNGT 5 cut(s) 209, 260, 432, 617, 1229
TaiI ACGT 1 cut(s) 1140
TaqI TCGA 4 cut(s) 147, 348, 937, 1085
TasI AATT 9 cut(s) 232, 607, 646, 722, 780, 905, 925, 1349, 1377
TatI WGTACW 1 cut(s) 538
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 4 cut(s) 155, 593, 979, 1186
TscAI CASTG 3 cut(s) 517, 622, 1403
TseFI GTSAC 1 cut(s) 758
TseI GCWGC 6 cut(s) 96, 774, 845, 947, 950, 1025
Tsp45I GTSAC 1 cut(s) 758
TspDTI ATGAA 7 cut(s) 7, 482, 878, 1088, 1230, 1309, 1362
TspRI CASTG 3 cut(s) 517, 622, 1403
Van91I CCANNNNNTGG 1 cut(s) 1269
Vha464I CTTAAG 2 cut(s) 701, 1169
VpaK11BI GGWCC 1 cut(s) 602
XapI RAATTY 1 cut(s) 1349
XbaI TCTAGA 1 cut(s) 196
XceI RCATGY 1 cut(s) 550
XspI CTAG 4 cut(s) 197, 297, 444, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.