MD01G1136100.v1.1

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014363
Physical Location & Seq
Reverse (-)
24502193 .. 24506790
4598 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1136100.v1.1.491

Sequence Viewer

Length: 2178 bp
ATGCCGTTTATCTCCAAAATTCAACGGCAGTCCGATTACAGACGCTTCCCTTCCTCCACTCCCATCGTCATCGACAATGGCGCCTCCTATTTTCGCATCGGGTGGGCAGGAGAGGCCGAGCCTCGTGTTATTTTCCGCAACATTGTTCAGAGGCCGCGCCACAAGGCCACCGGGGAAACTGTGACCATTGTTGGAGACCATGATCCTGCATTGTTGAAATACTTTGATTGCACGCGGTCGGGACCCCGCTCAGCTTTTGACAACAATGTTGTTTTCCAGTTTGAAATTATGGAATATATTCTTGACTTTGCCTTTGATCGGTTGGGTGCAAAAGGATCACAGATTGATCACCCGATCCTGATTACTGAATGCGTGTGCAACCCATTTCACTCTCGTAGTAAAATGGCTGAACTTTTGTTTGAGACATATGGAGTTCCATCTATAGCATATGGTGTCGATGCTGCATTCAGCTATAAGTATAATCAGCAGCATGGGGTTTGTGATAAAGATGGTCTTGCTATCTGTCCTGGTTTTACCACTACTCATGTTATTCCGTTTATTGATGGTGAGCCCGTATATAATGGAAGCTGTCGAACTAACATTGGTGGGTACCACATCACTGATTATATGAAGCAACTTCTCTCACTTAAATACCCTCATCACATGGCTAGGCTTACATGGGAGAAGGTTGAAGACCTGAAGATGGAGCACTGTTACATTGCTCTAGATTATGTGTCAGAAGCTCAGTTATTTCAGAGTGGGGCAAAGGAAGCTGAAGATAAAACAAGGATGTGGCAGCTTCCATGGGTTCCACCACCTACTGAGGAGCCCCCTTCCGAAGAAGAGATTGCAAGAAAGGCAGCCATTAAAGAGAAACAAGGTCAACGATTGCGAGAAATGGCTGAGGCGAAGAGATCTTCTAGAATAAATGAACTGGAGAATGAATCACACGGGTTGGAGTTTCTTCTAAAACAGCTTGAACAGGTGGAAGAGCCTGATATACCATCGTTCCTGTCAGCCACTGGCTATGTCTCCAAGAAGGAAGTAGAATCTGCCCTGGTGAAGGTAAGACAATCGTTACTGAAGACGAAAGGTGAACCAAAGGGTGAACAAGCTGAGCTTGATGAGAAGACGGATCCTGCAGGAATCGAAAGATTTCCTCTTTTAAATATACCTGACAACATGCTCACCCCTGAGCAGCTGAAGGAGAAAAAGAGACAGTTGTTTCTAAAAACCACATCTGAAGGTCGGCAGCGAGCTAAACAGAAGCGTCATGAAGAAGAATTAGAGCGGGAAAAGAGAAATCAACAAGATGAGAAAAAACGTTTAGAAAACCCGGAGCTTTATTTAGAGCAGTTGCATGCTAAATATAAAGATCTTTCAGAGAAAGTTGAACAGAGAAAACGGCTTAAGACAAATGGTGGGGGTCATACAAATGGAAATAATTTTTCTGGAAATGTTGGCCGTGGCGAGAGATTAAATGCTGCACAGAGGGAAAGGATGCGCCTTTTGACAACAGCAGCTTTTGATCGCGGCAAGGGTGAGGATACTTTTGGCGCAAAAGATGAAGACTGGCAATTATACAAACTCATGAGCAAAGATAACGATGATGATGATGAGGGACCAGATGTGGATGAAGCAGAGCTAGCGCGTCTCTCTTCTAGGCTCAAGGAAGTGGACCCGACATTTGTTCCCAAACCAGATATTGCAAACCCTCAATCTGCTGAAGCACCCAAGTTTCGTCCTCTTACCCAGGAAGATTTTCAAATTGTTTTCGGTGTTGAAAGGTTCAGGTGCCCAGAGGTCTTATTTTGTCCCAACTGGATTGGAATCGACCAGGCAGGCTTAGATGAGATGGCTGGGGTGTCGCTGAGGAGGCTGCCAACTAAGGGCCAAGAGCTGGAGGAGAGGCTAACCAGTTCGATATTTATGACTGGTGGGAGTAGTCTTTTTCCTGGCATGAGCGAGCGCTTGGAGGCAGGAATCCGGATGATTCGGCCGTGTGGTTCACCCATAAGGATAGTCAAGGCATTGGATCCAATTCTAGATGCATGGCGTGGAGCTTCCGCTTACGCCGCCGCCTCCCACTTCCCGACACAAACTTTCAGTAAGGAAGACTATTATGAGAAGGGTGAAGACTGCGTTCTCCGATACCAATTTCGGTATTATACATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

726

Amino Acids

82.8

Weight (kDa)

5.79

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 19 - 221 2e-30 Actin
Actin PF00022 589 - 718 4.9e-23 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12380 AT3G12380
fragaria_vesca FvH4_7g22790
malus_domestica MD01G1136100.v1.1
prunus_persica Prupe.2G237600_v2.0.a1 Prupe.2G237600_v2.0.a1
pyrus_communis pycom01g15690
rosa_chinensis RchiOBHm_Chr1g0367891
rosa_laevigata RLG00000027259
rosa_multiflora Rmu_ssc0000395.1_g000040
rosa_roxburghii Rroxscaffold_4G00289150
rosa_rugosa Rorug01G0337500
rosa_samantha Rh1AG344400 Rh1BG306400 Rh1CG322000 Rh1DG338600
rosa_wichuraiana Rw1G030660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 609
AccB1I GGYRCC 3 cut(s) 80, 609, 1794
AccB7I CCANNNNNTGG 1 cut(s) 1900
AccBSI CCGCTC 2 cut(s) 249, 1291
AccII CGCG 4 cut(s) 157, 235, 1533, 1651
AccIII TCCGGA 1 cut(s) 1986
AciI CCGC 9 cut(s) 136, 155, 235, 247, 1291, 1533, 2067, 2076, 2079
AclI AACGTT 1 cut(s) 1324
AclWI GGATC 7 cut(s) 197, 343, 349, 1130, 1143, 2030, 2043
AcoI YGGCCR 2 cut(s) 1462, 1997
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 6 cut(s) 719, 795, 1103, 1223, 1263, 1746
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 611
AfeI AGCGCT 1 cut(s) 1970
AfiI CCNNNNNNNGG 5 cut(s) 318, 703, 1063, 1889, 1900
AflII CTTAAG 1 cut(s) 1409
AgsI TTSAA 8 cut(s) 23, 217, 284, 692, 980, 1394, 1766, 1784
AjnI CCWGG 5 cut(s) 526, 1056, 1752, 1836, 1954
Alw21I GWGCWC 1 cut(s) 711
Alw26I GTCTC 5 cut(s) 189, 416, 1036, 1210, 1658
AlwI GGATC 7 cut(s) 197, 343, 349, 1130, 1143, 2030, 2043
AlwNI CAGNNNCTG 1 cut(s) 1022
Aor13HI TCCGGA 1 cut(s) 1986
Aor51HI AGCGCT 1 cut(s) 1970
AoxI GGCC 6 cut(s) 114, 152, 165, 1462, 1891, 1997
ApeKI GCWGC 9 cut(s) 461, 487, 796, 860, 1198, 1252, 1484, 1520, 1879
ApoI RAATTY 1 cut(s) 18
ArsI GACNNNNNNTTYG 4 cut(s) 296, 328, 1670, 1702
Asp700I GAANNNNTTC 3 cut(s) 297, 1155, 1761
Asp718I GGTACC 1 cut(s) 609
AspLEI GCGC 6 cut(s) 83, 159, 1506, 1559, 1651, 1971
AspS9I GGNCC 4 cut(s) 242, 1622, 1678, 1891
AsuC2I CCSGG 2 cut(s) 172, 1337
AsuHPI GGTGA 9 cut(s) 341, 578, 1072, 1106, 1118, 1180, 1553, 2001, 2144
AsuNHI GCTAGC 1 cut(s) 1645
AvaII GGWCC 3 cut(s) 242, 1622, 1678
BaeGI GKGCMC 1 cut(s) 1799
BamHI GGATCC 2 cut(s) 1135, 2035
BanI GGYRCC 3 cut(s) 80, 609, 1794
BanII GRGCYC 2 cut(s) 573, 831
BauI CACGAG 1 cut(s) 123
BbsI GAAGAC 6 cut(s) 699, 1091, 1136, 1575, 2121, 2142
Bbv12I GWGCWC 1 cut(s) 711
BbvCI CCTCAGC 2 cut(s) 903, 1871
BbvI GCAGC 9 cut(s) 448, 499, 808, 872, 1210, 1264, 1471, 1532, 1866
BccI CCATC 7 cut(s) 71, 445, 503, 557, 697, 1012, 1849
BceAI ACGGC 4 cut(s) 41, 1421, 1449, 1984
BciT130I CCWGG 5 cut(s) 528, 1058, 1754, 1838, 1956
BciVI GTATCC 1 cut(s) 1540
BclI TGATCA 1 cut(s) 346
BcnI CCSGG 2 cut(s) 172, 1337
BcoDI GTCTC 5 cut(s) 189, 416, 1036, 1210, 1658
BfaI CTAG 6 cut(s) 669, 725, 921, 1646, 1662, 2045
BfmI CTRYAG 2 cut(s) 441, 1140
BfoI RGCGCY 2 cut(s) 84, 1972
BfrI CTTAAG 1 cut(s) 1409
BfuI GTATCC 1 cut(s) 1540
BglII AGATCT 2 cut(s) 914, 1375
BlpI GCTNAGC 2 cut(s) 250, 1116
Bme1390I CCNGG 7 cut(s) 172, 528, 1058, 1337, 1754, 1838, 1956
Bme18I GGWCC 3 cut(s) 242, 1622, 1678
BmgT120I GGNCC 4 cut(s) 242, 1622, 1678, 1891
BmrFI CCNGG 7 cut(s) 172, 528, 1058, 1337, 1754, 1838, 1956
BmsI GCATC 4 cut(s) 105, 448, 1491, 2038
BmtI GCTAGC 1 cut(s) 1649
BpiI GAAGAC 6 cut(s) 699, 1091, 1136, 1575, 2121, 2142
BpmI CTGGAG 2 cut(s) 956, 1922
Bpu10I CCTNAGC 3 cut(s) 903, 1194, 1871
Bpu1102I GCTNAGC 2 cut(s) 250, 1116
BpuEI CTTGAG 1 cut(s) 1652
BpuMI CCSGG 2 cut(s) 172, 1337
BsaBI GATNNNNATC 1 cut(s) 1829
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 189
BsaJI CCNNGG 5 cut(s) 171, 803, 1056, 1465, 1752
BsaWI WCCGGW 1 cut(s) 1986
Bsc4I CCNNNNNNNGG 5 cut(s) 318, 703, 1063, 1889, 1900
Bse1I ACTGG 7 cut(s) 277, 939, 1027, 1577, 1826, 1917, 1939
Bse3DI GCAATG 1 cut(s) 717
Bse8I GATNNNNATC 1 cut(s) 1829
BseAI TCCGGA 1 cut(s) 1986
BseBI CCWGG 5 cut(s) 528, 1058, 1754, 1838, 1956
BseDI CCNNGG 5 cut(s) 171, 803, 1056, 1465, 1752
BseGI GGATG 4 cut(s) 795, 1506, 1639, 1995
BseJI GATNNNNATC 1 cut(s) 1829
BseLI CCNNNNNNNGG 5 cut(s) 318, 703, 1063, 1889, 1900
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 7 cut(s) 264, 758, 813, 894, 1107, 1185, 1862
BseNI ACTGG 7 cut(s) 277, 939, 1027, 1577, 1826, 1917, 1939
BseRI GAGGAG 3 cut(s) 839, 1888, 1919
BseSI GKGCMC 1 cut(s) 1799
BseX3I CGGCCG 1 cut(s) 1997
BseXI GCAGC 9 cut(s) 448, 499, 808, 872, 1210, 1264, 1471, 1532, 1866
BseYI CCCAGC 1 cut(s) 1859
BsgI GTGCAG 1 cut(s) 1470
Bsh1236I CGCG 4 cut(s) 157, 235, 1533, 1651
Bsh1285I CGRYCG 2 cut(s) 239, 2000
BshFI GGCC 6 cut(s) 116, 154, 167, 1464, 1893, 1999
BshNI GGYRCC 3 cut(s) 80, 609, 1794
BsiEI CGRYCG 2 cut(s) 239, 2000
BsiHKAI GWGCWC 1 cut(s) 711
BsiSI CCGG 3 cut(s) 171, 1337, 1987
BslFI GGGAC 3 cut(s) 255, 1635, 1800
BslI CCNNNNNNNGG 5 cut(s) 318, 703, 1063, 1889, 1900
BsmAI GTCTC 5 cut(s) 189, 416, 1036, 1210, 1658
BsmBI CGTCTC 1 cut(s) 1658
BsmFI GGGAC 3 cut(s) 255, 1635, 1800
BsmI GAATGC 2 cut(s) 374, 464
BsnI GGCC 6 cut(s) 116, 154, 167, 1464, 1893, 1999
Bso31I GGTCTC 1 cut(s) 189
Bsp1286I GDGCHC 4 cut(s) 573, 711, 831, 1799
Bsp13I TCCGGA 1 cut(s) 1986
Bsp1720I GCTNAGC 2 cut(s) 250, 1116
Bsp19I CCATGG 1 cut(s) 803
BspACI CCGC 9 cut(s) 136, 155, 235, 247, 1291, 1533, 2067, 2076, 2079
BspANI GGCC 6 cut(s) 116, 154, 167, 1464, 1893, 1999
BspCNI CTCAG 7 cut(s) 263, 757, 814, 895, 1108, 1186, 1863
BspEI TCCGGA 1 cut(s) 1986
BspFNI CGCG 4 cut(s) 157, 235, 1533, 1651
BspHI TCATGA 2 cut(s) 1273, 1590
BspMAI CTGCAG 1 cut(s) 1144
BspOI GCTAGC 1 cut(s) 1649
BspPI GGATC 7 cut(s) 197, 343, 349, 1130, 1143, 2030, 2043
BspQI GCTCTTC 1 cut(s) 984
BspT107I GGYRCC 3 cut(s) 80, 609, 1794
BspTI CTTAAG 1 cut(s) 1409
BspTNI GGTCTC 1 cut(s) 189
BsrBI CCGCTC 2 cut(s) 249, 1291
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 7 cut(s) 277, 939, 1027, 1577, 1826, 1917, 1939
BssECI CCNNGG 5 cut(s) 171, 803, 1056, 1465, 1752
BssNI GRCGYC 1 cut(s) 81
BssSI CACGAG 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 803
Bst2BI CACGAG 1 cut(s) 123
Bst2UI CCWGG 5 cut(s) 528, 1058, 1754, 1838, 1956
Bst4CI ACNGT 3 cut(s) 181, 713, 1221
Bst6I CTCTTC 4 cut(s) 837, 905, 984, 1663
BstACI GRCGYC 1 cut(s) 81
BstAFI CTTAAG 1 cut(s) 1409
BstC8I GCNNGC 6 cut(s) 233, 1257, 1362, 1647, 1843, 1967
BstDEI CTNAG 9 cut(s) 250, 744, 822, 903, 1116, 1194, 1846, 1871, 1887
BstDSI CCRYGG 2 cut(s) 803, 1465
BstENI CCTNNNNNAGG 1 cut(s) 1061
BstF5I GGATG 4 cut(s) 795, 1506, 1639, 1995
BstFNI CGCG 4 cut(s) 157, 235, 1533, 1651
BstH2I RGCGCY 2 cut(s) 84, 1972
BstHHI GCGC 6 cut(s) 83, 159, 1506, 1559, 1651, 1971
BstMAI GTCTC 5 cut(s) 189, 416, 1036, 1210, 1658
BstMCI CGRYCG 2 cut(s) 239, 2000
BstMWI GCNNNNNNNGC 6 cut(s) 113, 770, 857, 1646, 1876, 2075
BstNI CCWGG 5 cut(s) 528, 1058, 1754, 1838, 1956
BstNSI RCATGY 2 cut(s) 1186, 1364
BstSCI CCNGG 7 cut(s) 170, 526, 1056, 1335, 1752, 1836, 1954
BstSFI CTRYAG 2 cut(s) 441, 1140
BstSLI GKGCMC 1 cut(s) 1799
BstUI CGCG 4 cut(s) 157, 235, 1533, 1651
BstV1I GCAGC 9 cut(s) 448, 499, 808, 872, 1210, 1264, 1471, 1532, 1866
BstV2I GAAGAC 6 cut(s) 699, 1091, 1136, 1575, 2121, 2142
BstX2I RGATCY 4 cut(s) 914, 1135, 1375, 2035
BstYI RGATCY 4 cut(s) 914, 1135, 1375, 2035
BstZI CGGCCG 1 cut(s) 1997
BsuI GTATCC 1 cut(s) 1540
BsuRI GGCC 6 cut(s) 116, 154, 167, 1464, 1893, 1999
BtgI CCRYGG 2 cut(s) 803, 1465
BtsCI GGATG 4 cut(s) 795, 1506, 1639, 1995
BtsIMutI CAGTG 3 cut(s) 618, 709, 1020
Cac8I GCNNGC 6 cut(s) 233, 1257, 1362, 1647, 1843, 1967
CaiI CAGNNNCTG 1 cut(s) 1022
CciI TCATGA 2 cut(s) 1273, 1590
CfoI GCGC 6 cut(s) 83, 159, 1506, 1559, 1651, 1971
Cfr13I GGNCC 4 cut(s) 242, 1622, 1678, 1891
CseI GACGC 3 cut(s) 51, 1259, 1640
Csp6I GTAC 1 cut(s) 610
CviQI GTAC 1 cut(s) 610
DdeI CTNAG 9 cut(s) 250, 744, 822, 903, 1116, 1194, 1846, 1871, 1887
DinI GGCGCC 1 cut(s) 82
DraI TTTAAA 1 cut(s) 1167
EaeI YGGCCR 2 cut(s) 1462, 1997
EagI CGGCCG 1 cut(s) 1997
Eam1104I CTCTTC 4 cut(s) 837, 905, 984, 1663
EarI CTCTTC 4 cut(s) 837, 905, 984, 1663
EclXI CGGCCG 1 cut(s) 1997
Eco130I CCWWGG 1 cut(s) 803
Eco24I GRGCYC 2 cut(s) 573, 831
Eco31I GGTCTC 1 cut(s) 189
Eco47I GGWCC 3 cut(s) 242, 1622, 1678
Eco47III AGCGCT 1 cut(s) 1970
Eco52I CGGCCG 1 cut(s) 1997
Eco57I CTGAAG 6 cut(s) 719, 795, 1103, 1223, 1263, 1746
EcoNI CCTNNNNNAGG 1 cut(s) 1061
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 5 cut(s) 526, 1056, 1752, 1836, 1954
EcoT14I CCWWGG 1 cut(s) 803
EcoT22I ATGCAT 1 cut(s) 2053
EcoT38I GRGCYC 2 cut(s) 573, 831
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 803
Esp3I CGTCTC 1 cut(s) 1658
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 1104, 1136
FaqI GGGAC 3 cut(s) 255, 1635, 1800
FauI CCCGC 2 cut(s) 254, 1284
FauNDI CATATG 2 cut(s) 427, 448
FbaI TGATCA 1 cut(s) 346
FokI GGATG 4 cut(s) 802, 1513, 1646, 2002
FriOI GRGCYC 2 cut(s) 573, 831
FspBI CTAG 6 cut(s) 669, 725, 921, 1646, 1662, 2045
GlaI GCGC 6 cut(s) 82, 158, 1505, 1558, 1650, 1970
GsaI CCCAGC 1 cut(s) 1863
GsuI CTGGAG 2 cut(s) 956, 1922
HaeII RGCGCY 2 cut(s) 84, 1972
HaeIII GGCC 6 cut(s) 116, 154, 167, 1464, 1893, 1999
HapII CCGG 3 cut(s) 171, 1337, 1987
HgaI GACGC 3 cut(s) 51, 1259, 1640
HhaI GCGC 6 cut(s) 83, 159, 1506, 1559, 1651, 1971
Hin1I GRCGYC 1 cut(s) 81
Hin6I GCGC 6 cut(s) 81, 157, 1504, 1557, 1649, 1969
HinP1I GCGC 6 cut(s) 81, 157, 1504, 1557, 1649, 1969
HincII GTYRAC 1 cut(s) 884
HindII GTYRAC 1 cut(s) 884
HinfI GANTC 6 cut(s) 944, 1049, 1146, 1830, 1983, 1993
HpaII CCGG 3 cut(s) 171, 1337, 1987
HphI GGTGA 9 cut(s) 341, 578, 1072, 1106, 1118, 1180, 1553, 2001, 2144
Hpy166II GTNNAC 5 cut(s) 884, 1097, 1109, 1678, 2009
Hpy188I TCNGA 8 cut(s) 34, 150, 739, 756, 838, 1243, 1384, 2150
Hpy8I GTNNAC 5 cut(s) 884, 1097, 1109, 1678, 2009
HpyAV CCTTC 8 cut(s) 60, 679, 843, 1033, 1057, 1198, 1238, 2122
HpyCH4III ACNGT 3 cut(s) 181, 713, 1221
HpyCH4IV ACGT 1 cut(s) 1324
HpyF10VI GCNNNNNNNGC 6 cut(s) 113, 770, 857, 1646, 1876, 2075
HpyF3I CTNAG 9 cut(s) 250, 744, 822, 903, 1116, 1194, 1846, 1871, 1887
HpySE526I ACGT 1 cut(s) 1324
Hsp92I GRCGYC 1 cut(s) 81
HspAI GCGC 6 cut(s) 81, 157, 1504, 1557, 1649, 1969
KasI GGCGCC 1 cut(s) 80
KflI GGGWCCC 1 cut(s) 242
Kpn2I TCCGGA 1 cut(s) 1986
KpnI GGTACC 1 cut(s) 613
Ksp22I TGATCA 1 cut(s) 346
LguI GCTCTTC 1 cut(s) 984
LmnI GCTCC 4 cut(s) 706, 826, 1339, 2060
Lsp1109I GCAGC 9 cut(s) 448, 499, 808, 872, 1210, 1264, 1471, 1532, 1866
LweI GCATC 4 cut(s) 105, 448, 1491, 2038
MaeI CTAG 6 cut(s) 669, 725, 921, 1646, 1662, 2045
MaeII ACGT 1 cut(s) 1324
MaeIII GTNAC 3 cut(s) 181, 713, 1077
MbiI CCGCTC 2 cut(s) 249, 1291
MflI RGATCY 4 cut(s) 914, 1135, 1375, 2035
MhlI GDGCHC 4 cut(s) 573, 711, 831, 1799
MluCI AATT 8 cut(s) 18, 285, 1283, 1444, 1577, 1767, 2040, 2156
Mly113I GGCGCC 1 cut(s) 81
MmeI TCCRAC 2 cut(s) 172, 936
Mph1103I ATGCAT 1 cut(s) 2053
MroI TCCGGA 1 cut(s) 1986
MroXI GAANNNNTTC 3 cut(s) 297, 1155, 1761
MseI TTAA 5 cut(s) 648, 867, 1166, 1410, 1478
MslI CAYNNNNRTG 1 cut(s) 1434
MspA1I CMGCKG 1 cut(s) 1201
MspCI CTTAAG 1 cut(s) 1409
MspI CCGG 3 cut(s) 171, 1337, 1987
MspR9I CCNGG 7 cut(s) 172, 528, 1058, 1337, 1754, 1838, 1956
Mva1269I GAATGC 2 cut(s) 374, 464
MvaI CCWGG 5 cut(s) 528, 1058, 1754, 1838, 1956
MvnI CGCG 4 cut(s) 157, 235, 1533, 1651
MwoI GCNNNNNNNGC 6 cut(s) 113, 770, 857, 1646, 1876, 2075
NarI GGCGCC 1 cut(s) 81
NciI CCSGG 2 cut(s) 172, 1337
NcoI CCATGG 1 cut(s) 803
NdeI CATATG 2 cut(s) 427, 448
NheI GCTAGC 1 cut(s) 1645
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 181
NsiI ATGCAT 1 cut(s) 2053
NspI RCATGY 2 cut(s) 1186, 1364
PaeI GCATGC 1 cut(s) 1364
PagI TCATGA 2 cut(s) 1273, 1590
PciSI GCTCTTC 1 cut(s) 984
PctI GAATGC 2 cut(s) 374, 464
PdmI GAANNNNTTC 3 cut(s) 297, 1155, 1761
PfeI GAWTC 6 cut(s) 944, 1049, 1146, 1830, 1983, 1993
PflMI CCANNNNNTGG 1 cut(s) 1900
PluTI GGCGCC 1 cut(s) 84
PpuMI RGGWCCY 1 cut(s) 242
Psp1406I AACGTT 1 cut(s) 1324
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 5 cut(s) 526, 1056, 1752, 1836, 1954
PspFI CCCAGC 1 cut(s) 1859
PspGI CCWGG 5 cut(s) 526, 1056, 1752, 1836, 1954
PspPI GGNCC 4 cut(s) 242, 1622, 1678, 1891
PspPPI RGGWCCY 1 cut(s) 242
PstI CTGCAG 1 cut(s) 1144
PstNI CAGNNNCTG 1 cut(s) 1022
PsuI RGATCY 4 cut(s) 914, 1135, 1375, 2035
PvuII CAGCTG 1 cut(s) 1201
RsaI GTAC 1 cut(s) 611
RsaNI GTAC 1 cut(s) 610
RseI CAYNNNNRTG 1 cut(s) 1434
SapI GCTCTTC 1 cut(s) 984
SaqAI TTAA 5 cut(s) 648, 867, 1166, 1410, 1478
Sau96I GGNCC 4 cut(s) 242, 1622, 1678, 1891
SbfI CCTGCAGG 1 cut(s) 1144
ScrFI CCNGG 7 cut(s) 172, 528, 1058, 1337, 1754, 1838, 1956
SdaI CCTGCAGG 1 cut(s) 1144
SduI GDGCHC 4 cut(s) 573, 711, 831, 1799
SfaNI GCATC 4 cut(s) 105, 448, 1491, 2038
SfcI CTRYAG 2 cut(s) 441, 1140
SfoI GGCGCC 1 cut(s) 82
SinI GGWCC 3 cut(s) 242, 1622, 1678
SmiMI CAYNNNNRTG 1 cut(s) 1434
SmlI CTYRAG 2 cut(s) 1409, 1667
SmoI CTYRAG 2 cut(s) 1409, 1667
SphI GCATGC 1 cut(s) 1364
Sse8387I CCTGCAGG 1 cut(s) 1144
Sse9I AATT 8 cut(s) 18, 285, 1283, 1444, 1577, 1767, 2040, 2156
SsiI CCGC 9 cut(s) 136, 155, 235, 247, 1291, 1533, 2067, 2076, 2079
SspDI GGCGCC 1 cut(s) 80
SspMI CTAG 6 cut(s) 669, 725, 921, 1646, 1662, 2045
StyD4I CCNGG 7 cut(s) 170, 526, 1056, 1335, 1752, 1836, 1954
StyI CCWWGG 1 cut(s) 803
TaaI ACNGT 3 cut(s) 181, 713, 1221
TaiI ACGT 1 cut(s) 1327
TaqI TCGA 6 cut(s) 72, 456, 592, 1149, 1833, 1922
TasI AATT 8 cut(s) 18, 285, 1283, 1444, 1577, 1767, 2040, 2156
TauI GCSGC 4 cut(s) 157, 1536, 2078, 2081
TfiI GAWTC 6 cut(s) 944, 1049, 1146, 1830, 1983, 1993
Tru1I TTAA 5 cut(s) 648, 867, 1166, 1410, 1478
Tru9I TTAA 5 cut(s) 648, 867, 1166, 1410, 1478
TscAI CASTG 3 cut(s) 625, 716, 1027
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 9 cut(s) 461, 487, 796, 860, 1198, 1252, 1484, 1520, 1879
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 6 cut(s) 644, 945, 957, 1290, 1581, 1650
TspGWI ACGGA 2 cut(s) 543, 1148
TspRI CASTG 3 cut(s) 625, 716, 1027
Van91I CCANNNNNTGG 1 cut(s) 1900
Vha464I CTTAAG 1 cut(s) 1409
VpaK11BI GGWCC 3 cut(s) 242, 1622, 1678
XagI CCTNNNNNAGG 1 cut(s) 1061
XapI RAATTY 1 cut(s) 18
XbaI TCTAGA 3 cut(s) 724, 920, 2044
XceI RCATGY 2 cut(s) 1186, 1364
XmnI GAANNNNTTC 3 cut(s) 297, 1155, 1761
XspI CTAG 6 cut(s) 669, 725, 921, 1646, 1662, 2045
Zsp2I ATGCAT 1 cut(s) 2053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.