Rw1G030660

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014363
Physical Location & Seq
Reverse (-)
58563126 .. 58567985
4860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G030660.1

Sequence Viewer

Length: 2154 bp
ATGCCTTTCATATCCAAAATTCAGCGCCAGACCGATTACCGGCGCTTTCCTTCCTCCACGCCCATCGTCATCGACAATGGCGCCTCCTATTTCCGCATCGGATGGGCTGGAGAGCCAGAGCCTCGTGTTATCTTCCGCAACATTGTTCAGAGACCCCGCCACAAAGTCACTGGTGAAACTGTGACCATAGTTGGTGATCATGATGTTGCTTTGTTGAGATACTTTGATTGCACGCGCTCGGGACCTCGGTCGGCTTTTGATAACAATGTGGTTTACCAGTTTGAAATAATGGAATATATTCTTGACTTTGCTTTTGATCGACTGGGGGCTGATGGATCAAGGATTGATCATCCTATCCTGATTACAGAATGCGTATGCAACCCATTTCAGTCTCGTAGTAAGATGGCGGAACTACTATTTGACACATATGGAGTCCCATCGATAGCATATGGTGTTGATGCTGCATTCAGCTACAAGTATAATCAACATCATGGGGTTTGTAATAAAGATGGTCTTGCTATCTGTCCTGGATTTATGACTACTCATGTTATTCCGTTTATTGATGGTGAGCCTGAGTATAAAGGATGCTGTCGGACTAACATTGGTGGGTACCACATCACTGACTATCTGAAGCAACTTCTTTCACTTAAATACCCCCATCACATGGCTAGGTTTACATGGGAGAAGGTCGAAGACTTGAAGATGGAGCACTGTTATATTGCTCTAGATTATATGTCAGAAGCTCGACTATTTCAGAATGGAACCAAGGAAGCTGAAGATAAAACAAGGTCGTGGCAGCTCCCATGGGTTCCACCACCTACTGAGGAGCCTCCTTCTGAAGAAGAGATTGCAAGAAAGGCAGCCATAAAAGAGAAACAAGGTCAACGGTTGCGAGAAATGGCTGAAGCGAAAAGATCTTCTAGGATAAATGAACTAGAAAATGAATCGCATGGTTTGGAATTTCTGTTAAAGCAGCTTGAACAGGTGGAAGAGCATGATATACGATCTTTCTTGTCAGCGACTGGATATGTCTCCAGGAAGGAAGTAGAATCTGCCCTTTTGAAGGTAACACAATCTCTACAAAAAGCAAAGGGTGAACAAGCTGAGTTTGAGGAGAAACCAGATGCAGAAAGGTTTCATCTTTTAAATAAACCTGACCACGAGTGCACTCCAGAAGAGCTCAAGATGAAAAAGAGACAGGTGTTTCTAAAATCCACATCTGAAGGCCGTCAGCGAGCTAAACAGAAACGCCATGAAGAAGAACTGGAGCGAGAAAGGAGAAACCAACAAGATGAGCAAAGACGTTCAAAGAACCCCGAGCTTTATAAAGAGCAGTTACTTGCTAAATATAAAGATCTTTCTGAGAAAGTGGAACAGCGAAAACGACTCAAGACAAATGGGGGTCAGACAAATGGGAATAATATTTCTGGAAATGTTGGCCGTGGCGAGAGATTAAATGCTGCACAGAGGGAAAGGATGCGCCTTTTGACCACAGCAGCTTTTGATCGCGGCAAGGGTGAGGATACTTTTGGTGCAAGAGATGAAGATTGGCAACTATACAAGCTAATGAGCAAAGATAATGACGATGACGATGAGGGACCGGATGCGGATGAAGCAGAGTTGGCACGTCTAACTTCTAGGCTTCGGGAAGTAGACCCGACATTTGTTCCCAGATCAGAAAATGGAAACTCGCAATCACTTGAAACACCACGGACTCGTCCTCTTACTGAGGAAGATTTTCGGATAGATCTTGGTGTTGAAAGGTTCCGCTGCCCTGAAATTTTATTTCGTCCCAACTGGGTTGGGATCGATCAGGCAGGCTTAGATGAGATGGCTGGGGTCTCGCTAAGGAGGCTGCCATCTAAGAGCATAGAGCTGGAGGAGAGGCTAACCAGTTCAATTTTTTTAACTGGTGGGAGTAGTCTATTTCCTGGAATGAGTGAGCGCTTGGAAGCTGGAATCCGGATGATTACGCCATGTGGTTCACCTATAAGGGTTGTCAAAGCATTGAATCCAACTCTCGATGCATGGCGTGGTGCTTCTGCTTACGCTGCTTCCCCCCAGTTCCCAGCACAAACTTTCACTAAGCAGGAATATTATGAGAAGGGTGAAGACGTAGTCCGCAGATACCAATTTCGATACCATGTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

717

Amino Acids

82.58

Weight (kDa)

6.16

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 19 - 235 2.9e-28 Actin
Actin PF00022 581 - 710 1.9e-25 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12380 AT3G12380
fragaria_vesca FvH4_7g22790
malus_domestica MD01G1136100.v1.1
prunus_persica Prupe.2G237600_v2.0.a1 Prupe.2G237600_v2.0.a1
pyrus_communis pycom01g15690
rosa_chinensis RchiOBHm_Chr1g0367891
rosa_laevigata RLG00000027259
rosa_multiflora Rmu_ssc0000395.1_g000040
rosa_roxburghii Rroxscaffold_4G00289150
rosa_rugosa Rorug01G0337500
rosa_samantha Rh1AG344400 Rh1BG306400 Rh1CG322000 Rh1DG338600
rosa_wichuraiana Rw1G030660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1326
Acc65I GGTACC 1 cut(s) 609
AccB1I GGYRCC 2 cut(s) 80, 609
AccB7I CCANNNNNTGG 1 cut(s) 664
AccI GTMKAC 1 cut(s) 1653
AccII CGCG 2 cut(s) 235, 1509
AccIII TCCGGA 1 cut(s) 1962
AciI CCGC 8 cut(s) 94, 136, 157, 407, 1509, 1607, 1768, 2122
AclWI GGATC 2 cut(s) 343, 1814
AcoI YGGCCR 1 cut(s) 1438
AcsI RAATTY 3 cut(s) 18, 959, 1779
AcuI CTGAAG 5 cut(s) 650, 795, 858, 924, 1242
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 611
AfeI AGCGCT 1 cut(s) 1946
AfiI CCNNNNNNNGG 3 cut(s) 39, 664, 1063
AgsI TTSAA 9 cut(s) 284, 700, 980, 1063, 1308, 1703, 1760, 1899, 2011
AjiI CACGTC 1 cut(s) 1628
AjnI CCWGG 3 cut(s) 526, 1034, 1930
Alw21I GWGCWC 3 cut(s) 711, 1169, 1182
Alw26I GTCTC 5 cut(s) 145, 396, 1036, 1189, 1846
Alw44I GTGCAC 1 cut(s) 1165
AlwI GGATC 2 cut(s) 343, 1814
AlwNI CAGNNNCTG 1 cut(s) 1022
Ama87I CYCGRG 2 cut(s) 238, 1316
Aor13HI TCCGGA 1 cut(s) 1962
Aor51HI AGCGCT 1 cut(s) 1946
AoxI GGCC 2 cut(s) 1225, 1438
ApaLI GTGCAC 1 cut(s) 1165
ApeKI GCWGC 9 cut(s) 461, 796, 860, 973, 1460, 1496, 1770, 1855, 2051
ApoI RAATTY 3 cut(s) 18, 959, 1779
ArsI GACNNNNNNTTYG 4 cut(s) 296, 328, 1646, 1678
Asp700I GAANNNNTTC 3 cut(s) 297, 1134, 1737
Asp718I GGTACC 1 cut(s) 609
AspLEI GCGC 6 cut(s) 27, 45, 83, 237, 1482, 1947
AspS9I GGNCC 2 cut(s) 242, 1598
AsuHPI GGTGA 7 cut(s) 185, 206, 578, 1106, 1529, 1977, 2120
AvaI CYCGRG 2 cut(s) 238, 1316
AvaII GGWCC 2 cut(s) 242, 1598
BaeGI GKGCMC 1 cut(s) 1169
BanI GGYRCC 2 cut(s) 80, 609
BanII GRGCYC 1 cut(s) 1182
BauI CACGAG 2 cut(s) 123, 1160
BbsI GAAGAC 2 cut(s) 699, 2118
Bbv12I GWGCWC 3 cut(s) 711, 1169, 1182
BbvI GCAGC 9 cut(s) 448, 808, 872, 985, 1447, 1508, 1757, 1842, 2038
BceAI ACGGC 2 cut(s) 1212, 1425
BciT130I CCWGG 3 cut(s) 528, 1036, 1932
BciVI GTATCC 1 cut(s) 1516
BclI TGATCA 2 cut(s) 196, 346
BcoDI GTCTC 5 cut(s) 145, 396, 1036, 1189, 1846
BfaI CTAG 5 cut(s) 669, 725, 921, 935, 1638
BfoI RGCGCY 4 cut(s) 28, 46, 84, 1948
BfuI GTATCC 1 cut(s) 1516
BglII AGATCT 3 cut(s) 914, 1354, 1747
Bme1390I CCNGG 3 cut(s) 528, 1036, 1932
Bme18I GGWCC 2 cut(s) 242, 1598
BmeT110I CYCGRG 2 cut(s) 238, 1316
BmgBI CACGTC 1 cut(s) 1628
BmgT120I GGNCC 2 cut(s) 242, 1598
BmiI GGNNCC 8 cut(s) 82, 243, 611, 763, 810, 828, 1599, 1766
BmrFI CCNGG 3 cut(s) 528, 1036, 1932
BmrI ACTGGG 3 cut(s) 332, 1807, 2056
BmsI GCATC 7 cut(s) 105, 448, 575, 1114, 1467, 1594, 2014
BmuI ACTGGG 3 cut(s) 332, 1807, 2056
BoxI GACNNNNGTC 1 cut(s) 247
BpiI GAAGAC 2 cut(s) 699, 2118
BpmI CTGGAG 5 cut(s) 129, 1018, 1155, 1286, 1898
Bpu10I CCTNAGC 1 cut(s) 1847
BpuEI CTTGAG 2 cut(s) 1166, 1373
Bsa29I ATCGAT 2 cut(s) 440, 1809
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 2 cut(s) 145, 1846
BsaJI CCNNGG 5 cut(s) 245, 765, 803, 1441, 1709
BsaWI WCCGGW 2 cut(s) 1600, 1962
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 664, 1063
Bse118I RCCGGY 1 cut(s) 39
Bse1I ACTGG 9 cut(s) 175, 277, 327, 1027, 1269, 1802, 1893, 1915, 2062
BseAI TCCGGA 1 cut(s) 1962
BseBI CCWGG 3 cut(s) 528, 1036, 1932
BseCI ATCGAT 2 cut(s) 440, 1809
BseDI CCNNGG 5 cut(s) 245, 765, 803, 1441, 1709
BseGI GGATG 7 cut(s) 107, 349, 590, 1482, 1609, 1615, 1971
BseLI CCNNNNNNNGG 3 cut(s) 39, 664, 1063
BseMII CTCAG 5 cut(s) 564, 813, 1095, 1353, 1719
BseNI ACTGG 9 cut(s) 175, 277, 327, 1027, 1269, 1802, 1893, 1915, 2062
BseRI GAGGAG 3 cut(s) 839, 1127, 1895
BseSI GKGCMC 1 cut(s) 1169
BseXI GCAGC 9 cut(s) 448, 808, 872, 985, 1447, 1508, 1757, 1842, 2038
BseYI CCCAGC 2 cut(s) 1835, 2068
BsgI GTGCAG 1 cut(s) 1446
Bsh1236I CGCG 2 cut(s) 235, 1509
Bsh1285I CGRYCG 1 cut(s) 251
BshFI GGCC 2 cut(s) 1227, 1440
BshNI GGYRCC 2 cut(s) 80, 609
BshVI ATCGAT 2 cut(s) 440, 1809
BsiEI CGRYCG 1 cut(s) 251
BsiHKAI GWGCWC 3 cut(s) 711, 1169, 1182
BsiHKCI CYCGRG 2 cut(s) 238, 1316
BsiSI CCGG 3 cut(s) 40, 1601, 1963
BslFI GGGAC 4 cut(s) 255, 419, 1611, 1776
BslI CCNNNNNNNGG 3 cut(s) 39, 664, 1063
BsmAI GTCTC 5 cut(s) 145, 396, 1036, 1189, 1846
BsmFI GGGAC 4 cut(s) 255, 419, 1611, 1776
BsmI GAATGC 2 cut(s) 374, 464
BsnI GGCC 2 cut(s) 1227, 1440
Bso31I GGTCTC 2 cut(s) 145, 1846
BsoBI CYCGRG 2 cut(s) 238, 1316
Bsp1286I GDGCHC 3 cut(s) 711, 1169, 1182
Bsp13I TCCGGA 1 cut(s) 1962
Bsp19I CCATGG 1 cut(s) 803
BspACI CCGC 8 cut(s) 94, 136, 157, 407, 1509, 1607, 1768, 2122
BspANI GGCC 2 cut(s) 1227, 1440
BspCNI CTCAG 5 cut(s) 565, 814, 1096, 1354, 1720
BspDI ATCGAT 2 cut(s) 440, 1809
BspEI TCCGGA 1 cut(s) 1962
BspFNI CGCG 2 cut(s) 235, 1509
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 8 cut(s) 82, 243, 611, 763, 810, 828, 1599, 1766
BspPI GGATC 2 cut(s) 343, 1814
BspQI GCTCTTC 2 cut(s) 984, 1170
BspT107I GGYRCC 2 cut(s) 80, 609
BspTNI GGTCTC 2 cut(s) 145, 1846
BsrFI RCCGGY 1 cut(s) 39
BsrI ACTGG 9 cut(s) 175, 277, 327, 1027, 1269, 1802, 1893, 1915, 2062
BssAI RCCGGY 1 cut(s) 39
BssECI CCNNGG 5 cut(s) 245, 765, 803, 1441, 1709
BssNI GRCGYC 1 cut(s) 81
BssSI CACGAG 2 cut(s) 123, 1160
BssT1I CCWWGG 2 cut(s) 765, 803
Bst2BI CACGAG 2 cut(s) 123, 1160
Bst2UI CCWGG 3 cut(s) 528, 1036, 1932
Bst4CI ACNGT 3 cut(s) 181, 713, 888
Bst6I CTCTTC 3 cut(s) 837, 984, 1170
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 3 cut(s) 233, 1236, 1819
BstDEI CTNAG 9 cut(s) 573, 822, 1104, 1362, 1728, 1822, 1847, 1863, 2085
BstDSI CCRYGG 3 cut(s) 803, 1441, 1709
BstENI CCTNNNNNAGG 1 cut(s) 1061
BstF5I GGATG 7 cut(s) 107, 349, 590, 1482, 1609, 1615, 1971
BstFNI CGCG 2 cut(s) 235, 1509
BstH2I RGCGCY 4 cut(s) 28, 46, 84, 1948
BstHHI GCGC 6 cut(s) 27, 45, 83, 237, 1482, 1947
BstMAI GTCTC 5 cut(s) 145, 396, 1036, 1189, 1846
BstMCI CGRYCG 1 cut(s) 251
BstMWI GCNNNNNNNGC 5 cut(s) 857, 1613, 1622, 1852, 2051
BstNI CCWGG 3 cut(s) 528, 1036, 1932
BstPAI GACNNNNGTC 1 cut(s) 247
BstSCI CCNGG 3 cut(s) 526, 1034, 1930
BstSLI GKGCMC 1 cut(s) 1169
BstUI CGCG 2 cut(s) 235, 1509
BstV1I GCAGC 9 cut(s) 448, 808, 872, 985, 1447, 1508, 1757, 1842, 2038
BstV2I GAAGAC 2 cut(s) 699, 2118
BstX2I RGATCY 3 cut(s) 914, 1354, 1747
BstYI RGATCY 3 cut(s) 914, 1354, 1747
Bsu15I ATCGAT 2 cut(s) 440, 1809
BsuI GTATCC 1 cut(s) 1516
BsuRI GGCC 2 cut(s) 1227, 1440
BsuTUI ATCGAT 2 cut(s) 440, 1809
BtgI CCRYGG 3 cut(s) 803, 1441, 1709
BtrI CACGTC 1 cut(s) 1628
BtsCI GGATG 7 cut(s) 107, 349, 590, 1482, 1609, 1615, 1971
BtsIMutI CAGTG 3 cut(s) 168, 618, 709
Cac8I GCNNGC 3 cut(s) 233, 1236, 1819
CaiI CAGNNNCTG 1 cut(s) 1022
CciI TCATGA 1 cut(s) 199
CfoI GCGC 6 cut(s) 27, 45, 83, 237, 1482, 1947
Cfr10I RCCGGY 1 cut(s) 39
Cfr13I GGNCC 2 cut(s) 242, 1598
ClaI ATCGAT 2 cut(s) 440, 1809
Csp6I GTAC 1 cut(s) 610
CviQI GTAC 1 cut(s) 610
DdeI CTNAG 9 cut(s) 573, 822, 1104, 1362, 1728, 1822, 1847, 1863, 2085
DinI GGCGCC 1 cut(s) 82
DraI TTTAAA 1 cut(s) 1146
EaeI YGGCCR 1 cut(s) 1438
Eam1104I CTCTTC 3 cut(s) 837, 984, 1170
EarI CTCTTC 3 cut(s) 837, 984, 1170
EciI GGCGGA 1 cut(s) 422
Ecl136II GAGCTC 1 cut(s) 1180
Eco130I CCWWGG 2 cut(s) 765, 803
Eco24I GRGCYC 1 cut(s) 1182
Eco31I GGTCTC 2 cut(s) 145, 1846
Eco47I GGWCC 2 cut(s) 242, 1598
Eco47III AGCGCT 1 cut(s) 1946
Eco53kI GAGCTC 1 cut(s) 1180
Eco57I CTGAAG 5 cut(s) 650, 795, 858, 924, 1242
Eco88I CYCGRG 2 cut(s) 238, 1316
EcoICRI GAGCTC 1 cut(s) 1180
EcoNI CCTNNNNNAGG 1 cut(s) 1061
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 3 cut(s) 526, 1034, 1930
EcoT14I CCWWGG 2 cut(s) 765, 803
EcoT22I ATGCAT 1 cut(s) 2029
EcoT38I GRGCYC 1 cut(s) 1182
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
ErhI CCWWGG 2 cut(s) 765, 803
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 4 cut(s) 255, 419, 1611, 1776
FauI CCCGC 1 cut(s) 164
FauNDI CATATG 2 cut(s) 427, 448
FbaI TGATCA 2 cut(s) 196, 346
FblI GTMKAC 1 cut(s) 1653
FokI GGATG 7 cut(s) 114, 336, 597, 1489, 1616, 1622, 1978
FriOI GRGCYC 1 cut(s) 1182
FspBI CTAG 5 cut(s) 669, 725, 921, 935, 1638
GlaI GCGC 6 cut(s) 26, 44, 82, 236, 1481, 1946
GsaI CCCAGC 2 cut(s) 1839, 2072
GsuI CTGGAG 5 cut(s) 129, 1018, 1155, 1286, 1898
HaeII RGCGCY 4 cut(s) 28, 46, 84, 1948
HaeIII GGCC 2 cut(s) 1227, 1440
HapII CCGG 3 cut(s) 40, 1601, 1963
HhaI GCGC 6 cut(s) 27, 45, 83, 237, 1482, 1947
Hin1I GRCGYC 1 cut(s) 81
Hin6I GCGC 6 cut(s) 25, 43, 81, 235, 1480, 1945
HinP1I GCGC 6 cut(s) 25, 43, 81, 235, 1480, 1945
HincII GTYRAC 1 cut(s) 884
HindII GTYRAC 1 cut(s) 884
HinfI GANTC 7 cut(s) 432, 944, 1049, 1386, 1714, 1959, 2011
HpaII CCGG 3 cut(s) 40, 1601, 1963
HphI GGTGA 7 cut(s) 185, 206, 578, 1106, 1529, 1977, 2120
Hpy166II GTNNAC 7 cut(s) 274, 675, 884, 1097, 1167, 1654, 1985
Hpy8I GTNNAC 7 cut(s) 274, 675, 884, 1097, 1167, 1654, 1985
HpyAV CCTTC 7 cut(s) 60, 679, 843, 1033, 1057, 1217, 2098
HpyCH4III ACNGT 3 cut(s) 181, 713, 888
HpyCH4IV ACGT 3 cut(s) 1303, 1627, 2115
HpyCH4V TGCA 9 cut(s) 231, 378, 464, 851, 1127, 1167, 1463, 1535, 2027
HpyF10VI GCNNNNNNNGC 5 cut(s) 857, 1613, 1622, 1852, 2051
HpyF3I CTNAG 9 cut(s) 573, 822, 1104, 1362, 1728, 1822, 1847, 1863, 2085
HpySE526I ACGT 3 cut(s) 1303, 1627, 2115
Hsp92I GRCGYC 1 cut(s) 81
HspAI GCGC 6 cut(s) 25, 43, 81, 235, 1480, 1945
KasI GGCGCC 1 cut(s) 80
Kpn2I TCCGGA 1 cut(s) 1962
KpnI GGTACC 1 cut(s) 613
Ksp22I TGATCA 2 cut(s) 196, 346
LguI GCTCTTC 2 cut(s) 984, 1170
LmnI GCTCC 4 cut(s) 706, 804, 826, 1267
Lsp1109I GCAGC 9 cut(s) 448, 808, 872, 985, 1447, 1508, 1757, 1842, 2038
LweI GCATC 7 cut(s) 105, 448, 575, 1114, 1467, 1594, 2014
MaeI CTAG 5 cut(s) 669, 725, 921, 935, 1638
MaeII ACGT 3 cut(s) 1303, 1627, 2115
MaeIII GTNAC 4 cut(s) 166, 181, 1066, 1335
MflI RGATCY 3 cut(s) 914, 1354, 1747
MhlI GDGCHC 3 cut(s) 711, 1169, 1182
MluCI AATT 5 cut(s) 18, 959, 1779, 1899, 2132
Mly113I GGCGCC 1 cut(s) 81
MlyI GAGTC 3 cut(s) 441, 1380, 1708
MmeI TCCRAC 2 cut(s) 572, 2039
Mph1103I ATGCAT 1 cut(s) 2029
MroI TCCGGA 1 cut(s) 1962
MroXI GAANNNNTTC 3 cut(s) 297, 1134, 1737
MseI TTAA 5 cut(s) 648, 968, 1145, 1454, 1907
MspA1I CMGCKG 1 cut(s) 1770
MspI CCGG 3 cut(s) 40, 1601, 1963
MspR9I CCNGG 3 cut(s) 528, 1036, 1932
Mva1269I GAATGC 2 cut(s) 374, 464
MvaI CCWGG 3 cut(s) 528, 1036, 1932
MvnI CGCG 2 cut(s) 235, 1509
MwoI GCNNNNNNNGC 5 cut(s) 857, 1613, 1622, 1852, 2051
NarI GGCGCC 1 cut(s) 81
NcoI CCATGG 1 cut(s) 803
NdeI CATATG 2 cut(s) 427, 448
NlaIV GGNNCC 8 cut(s) 82, 243, 611, 763, 810, 828, 1599, 1766
NmuCI GTSAC 2 cut(s) 166, 181
NsiI ATGCAT 1 cut(s) 2029
PagI TCATGA 1 cut(s) 199
PciSI GCTCTTC 2 cut(s) 984, 1170
PctI GAATGC 2 cut(s) 374, 464
PdmI GAANNNNTTC 3 cut(s) 297, 1134, 1737
PfeI GAWTC 4 cut(s) 944, 1049, 1959, 2011
PflFI GACNNNGTC 1 cut(s) 2117
PflMI CCANNNNNTGG 1 cut(s) 664
PfoI TCCNGGA 3 cut(s) 526, 1034, 1930
PleI GAGTC 3 cut(s) 440, 1380, 1708
PluTI GGCGCC 1 cut(s) 84
PpsI GAGTC 3 cut(s) 440, 1380, 1708
PpuMI RGGWCCY 1 cut(s) 242
PshAI GACNNNNGTC 1 cut(s) 247
PsiI TTATAA 1 cut(s) 1326
Psp124BI GAGCTC 1 cut(s) 1182
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 3 cut(s) 526, 1034, 1930
PspFI CCCAGC 2 cut(s) 1835, 2068
PspGI CCWGG 3 cut(s) 526, 1034, 1930
PspN4I GGNNCC 8 cut(s) 82, 243, 611, 763, 810, 828, 1599, 1766
PspPI GGNCC 2 cut(s) 242, 1598
PspPPI RGGWCCY 1 cut(s) 242
PstNI CAGNNNCTG 1 cut(s) 1022
PsuI RGATCY 3 cut(s) 914, 1354, 1747
PsyI GACNNNGTC 1 cut(s) 2117
RsaI GTAC 1 cut(s) 611
RsaNI GTAC 1 cut(s) 610
SacI GAGCTC 1 cut(s) 1182
SapI GCTCTTC 2 cut(s) 984, 1170
SaqAI TTAA 5 cut(s) 648, 968, 1145, 1454, 1907
Sau96I GGNCC 2 cut(s) 242, 1598
SchI GAGTC 3 cut(s) 441, 1380, 1708
ScrFI CCNGG 3 cut(s) 528, 1036, 1932
SduI GDGCHC 3 cut(s) 711, 1169, 1182
SfaNI GCATC 7 cut(s) 105, 448, 575, 1114, 1467, 1594, 2014
SfoI GGCGCC 1 cut(s) 82
SinI GGWCC 2 cut(s) 242, 1598
SmlI CTYRAG 2 cut(s) 1181, 1388
SmoI CTYRAG 2 cut(s) 1181, 1388
Sse9I AATT 5 cut(s) 18, 959, 1779, 1899, 2132
SsiI CCGC 8 cut(s) 94, 136, 157, 407, 1509, 1607, 1768, 2122
SspDI GGCGCC 1 cut(s) 80
SspI AATATT 2 cut(s) 1423, 2096
SspMI CTAG 5 cut(s) 669, 725, 921, 935, 1638
SstI GAGCTC 1 cut(s) 1182
StyD4I CCNGG 3 cut(s) 526, 1034, 1930
StyI CCWWGG 2 cut(s) 765, 803
TaaI ACNGT 3 cut(s) 181, 713, 888
TaiI ACGT 3 cut(s) 1306, 1630, 2118
TaqI TCGA 8 cut(s) 72, 319, 440, 690, 745, 1809, 2022, 2137
TaqII GACCGA 2 cut(s) 47, 237
TasI AATT 5 cut(s) 18, 959, 1779, 1899, 2132
TauI GCSGC 1 cut(s) 1512
TfiI GAWTC 4 cut(s) 944, 1049, 1959, 2011
Tru1I TTAA 5 cut(s) 648, 968, 1145, 1454, 1907
Tru9I TTAA 5 cut(s) 648, 968, 1145, 1454, 1907
TscAI CASTG 3 cut(s) 175, 625, 716
TseFI GTSAC 2 cut(s) 166, 181
TseI GCWGC 9 cut(s) 461, 796, 860, 973, 1460, 1496, 1770, 1855, 2051
Tsp45I GTSAC 2 cut(s) 166, 181
TspDTI ATGAA 7 cut(s) 945, 957, 1127, 1202, 1269, 1557, 1626
TspGWI ACGGA 2 cut(s) 543, 1726
TspRI CASTG 3 cut(s) 175, 625, 716
Tth111I GACNNNGTC 1 cut(s) 2117
Van91I CCANNNNNTGG 1 cut(s) 664
VneI GTGCAC 1 cut(s) 1165
VpaK11BI GGWCC 2 cut(s) 242, 1598
XagI CCTNNNNNAGG 1 cut(s) 1061
XapI RAATTY 3 cut(s) 18, 959, 1779
XbaI TCTAGA 1 cut(s) 724
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 1653
XmnI GAANNNNTTC 3 cut(s) 297, 1134, 1737
XspI CTAG 5 cut(s) 669, 725, 921, 935, 1638
Zsp2I ATGCAT 1 cut(s) 2029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.