RLG00000027259

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014363
Physical Location & Seq
Forward (+)
10087670 .. 10095700
8031 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027259

Sequence Viewer

Length: 3360 bp
ATGCCTTTCATATCCAAAATTCAGCGCCAGGCCGATTACCGGCGCTTCCCTTCCTCCACCCCCATCGTCATCGACAATGGCGCCTCCTATTTCCGCATCGGATGGGCCGGAGAGCCAGAGCCTCGTGTTATCTTCCGCAACATTGTTCAGAGACCCCGTCACAAAGTCACTGGTGAAACTGTGACCATAGTTGGTGATCATGATGTTGCTTTGTTGAGATACTTTGATTGCACGCGCTCGGGACCTCGGTCGGCTTTTGATAACAATGTGGTTTACCAGTTTGAAATAATGGAATATATTCTTGACTTCGCTTTTGATCGACTGGGGGCTGATGGATCCAAGATTGATCATCCTATCCTGATTACAGAATGCGTATGCAACCCATTTCAGTCTCGTAGTAAGATGGCGGAACTATTATTTGACACATATGGAGTCCCATCGATAGCATATGGTGTTGATGCTGCATTCAGCTACAAGTATAATCAACATCATGGGGTTTGTAATAAAGACGGTCTTGCTATCTGTCCTGGATTTATGACTACTCATGTTATTCCGTTTATTGATGGTGAGCCTGAGTATAAAGGATGCTGTCGGACTAACATTGGTGGGTACCACATCACTGACTATCTGAAGCAACTTCTTTCACTTAAATACCCCCATCACATGGCTCGGTTTACATGGGAGAAGGTCGAAGACTTGAAGATGGAGCACTGTTATATTGCTCTAGATTATATGTCAGAAGCTCGACTATTTCAGAATGGAACCAAGGAAGCTGAAGATAAAACAAGGTCGTGGCAGCTCCCATGGGTTCCACCACCTACTGAGGAGCCTCCTTCTGAAGAAGAGATTGCAAGAAAGGCAGCCATAAAAGAGAAACAAGGTCAACGGTTGCGAGAAATGGCTGAAGCGAAAAGATCTTCTAGGATAAATGAACTAGAAAATGAATCGCATGGTTTGGAATTTCTGTTAAAGCAGCTTGAACAGGTGGAAGAGCATGATATACGATCTTTCTTGTCAGCGACTGGATATGTCTCCAGGAAGGAAGTAGAATCTGCCCTTTTGAAGGTTACACAATCTCTACAAAAAGCAAAGGGTGAACAGGCTGAGTTTGAGGAGAAACCAGATGCAGAAAGGTTTCATCTTTTAAATAAACCTGACCACGAGTGCACTCCAGAAGAGCTCAAGATGAAAAAGAGACAGGTGTTTCTAAAATCCACATCTGAAGGCCGTCAGCGAGCTAAACAGAAACGCCATGAAGAAGAACTGGAGCGAGAAAGGAGAAACCAACAAGATGAGCAAAGACGTTCAAAGAACCCCGAGCTTTATAAAGAGCAGTTGCTTGCTAAATATAAAGATCTTTCTGAGAAAGTGGAACAGCGAAAACGACTCAAGACAAATGGGGGTCAGACAAATGGGAATAATATATCTGGAAATGTTGGCCGTGGCGAGAGATTAAATGCTGCACAGAGGGAAAGGATGCGCCTTTTGACCACAGCAGCTTTTGATCGCGGCAAAGGTGAGGATACTTTTGGTGCAAGAGATGAAGATTGGCAACTATACAAGCTAATGAGCAAAGATAATGACGATGATGATGAGGGACCGGATGCGGATGAAGCAGAATTGGCACGTCTAACTTCTAGGCTTCGGGAAGTAGACCCGACATTTGTTCCCAGATCAGAAAATGGAAACTCGCAATCACTTGAAACACCGCGGACTCGTCCTCTTACTGAGGAAGATTTTAGGATAGATCTTGGTGTTGAAAGGTTCCGCTGCCCTGAAATTTTATTTCGTCCCAACTGGGTTGGGATCGATCAGGCAGGCTTAGATGAGATGGCTGGGGCCTCGCTAAGGAGGCTGCCATCTAAGAGCACAGAGCTGGAGGAGAGGCTAACCAGTTCGATTTTTTTAACTGGTGGGAGTAGTCTATTTCCTGGAATGAGTGAGCGCTTGGAAGCTGGAATCCGGATGATTACGCCATGTGGTTCACCTATAAGGGTCGTCAAAGCATTGAATCCAACTCTCGATGCATGGCGTGGTGCTTCTGCTTACGCTGCTTCCCCCCGGTTCCCAGCACAAACTTTCAGTAAGCAAGAATATTATGAGAAGGGTGAAGACGTAGTCCGCAGATACCAATTTCGATACCATAGCACCCAAGTCTTCGTCTTCCTCCTCACTCCAAAACCCCCAGAGCCCTCCTTGCTCCCACACAACGAGGCCTTTTTCCGATCTTGGGTTTCTTCCACCTCCATCAATTTTCCAAACCCAACAAAACCCAGAAATGGATTCACCGCAAACTCCCCGGAAAACCGGGCTCAACCTCCCGACCGGGATTCCCAGCAAGAACATGTTGCGGTTCAAGACGGAGCATTCCGGGCCAAACTCGCCGTATTCGCCGTCGATTTTGAGGTTCTGGATGCTCCATCACTTCAAGATGACTTTTACTTGAATCTGGTGGACTGGTCCTCGCAAAATGTCCTTGCAGTTGGATTGGGAACCTCAGTTTATTTATGGAATGCATCAAACAGCAAGGTGACAAGACTGTGTGACTTGGGGCCTAATGATGGTGTCTGCTCTGTTCAATGGACCAAGGAGGGTTCGTATTTATCAATTGGTACAAACGGTGGCCAAGTTCAGGTTTGGGATGGGACTCAATGCAAGAAGGTCCGAACAATGAGTGGACATCAAACAAGAACTGGTGTTTTGGCATGGAACTCAAGGATACTAGCTTCAGGAAGCCGAGACCGGAATATCCTTCAACATGATATGCGAGTCTCAAATGACTGCATCAACAAGCTTGTTGGCCACAAGTCTGAGGTATGCGGGCTGAAATGGTCCAACGATGACAGGGAACTTGCTTCTGGTGGCAATGATAATCAGCTCTTGGTGTGGAACCAGCACTCTCAGCAACCTGCGTTGAGACTAACAGAGCACACAGCTGCAGTAAAGGCCATTGCTTGGTCACCCCACCAGAGCGGCCTTCTTGCTTCTGGAGGTGGAACTGCTGACAGATGTATACGCTTCTGGAACACTGCAAATGGCCACCAGTTGAACAGCGTTGACACGGGAAGCCAGGTTTGCAATCTCGCATGGAGTAAGAATGTGAATGAGATAGTTAGCACTCATGGATATTCCCAAAACCAAATTATGGTCTGGAAGTATCCATCCATGTCAAAGGTTGCCACTCTAACCGGCCATAGCATGCGAGTTCTATACCTTGCAATGTCACCAGATGGTCAGACAATAGTCACTGGTGCTGGGGATGAAACTCTCAGATTCTGGAATGTGTTTCCTTCGGTAAAAACTCCGACACCATGCAAAGAAACAGGTCTTTGGTCACTGGGACGAACTCAAATCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

1120

Amino Acids

126.77

Weight (kDa)

6.75

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 19 - 235 5.3e-28 Actin
Actin PF00022 581 - 710 1.1e-25 Actin
WD40_CDC20-Fz PF24807 804 - 1093 1.6e-128 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 816 - 905 1.8e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 819 - 921 2.2e-08 THOC3 beta-propeller domain
Beta-prop_WDR19_1st PF23389 819 - 917 2.7e-11 WDR19 first beta-propeller
Beta-prop_WDR3_2nd PF25172 822 - 1094 6e-18 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 822 - 991 3.1e-18 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 825 - 922 4.9e-10 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 830 - 923 4.2e-09 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 851 - 1094 1.1e-15 MABP1/WDR62 second WD40 domain
WDR55 PF24796 854 - 1098 5.3e-10 WDR55
WD40_Prp19 PF24814 904 - 1094 3e-20 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 905 - 1039 1.2e-17 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 920 - 1029 2.6e-08 TEP-1 second beta-propeller
WD40_Gbeta PF25391 924 - 1098 6.6e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 925 - 1039 4.4e-17 WDR3 first beta-propeller domain
WD40 PF00400 927 - 964 1.3e-06 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 928 - 1018 7e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 932 - 1041 9.6e-06 EIF3I
WD40_WDHD1_1st PF24817 934 - 994 6.9e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 935 - 1040 2e-21 THOC3 beta-propeller domain
Beta-prop_WDR19_1st PF23389 942 - 1094 1e-05 WDR19 first beta-propeller
WD40_MABP1-WDR62_1st PF24780 974 - 1096 9.3e-06 MABP1/WDR62 first WD40 domain
Beta-prop_EML PF23409 975 - 1096 2.2e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 976 - 1095 4.4e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 1001 - 1106 9.8e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 1001 - 1095 1.3e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 1023 - 1096 1.2e-08 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1044 - 1095 3e-08 THOC3 beta-propeller domain
WD40 PF00400 1058 - 1094 8.6e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12380 AT3G12380
fragaria_vesca FvH4_7g22790
malus_domestica MD01G1136100.v1.1
prunus_persica Prupe.2G237600_v2.0.a1 Prupe.2G237600_v2.0.a1
pyrus_communis pycom01g15690
rosa_chinensis RchiOBHm_Chr1g0367891
rosa_laevigata RLG00000027259
rosa_multiflora Rmu_ssc0000395.1_g000040
rosa_roxburghii Rroxscaffold_4G00289150
rosa_rugosa Rorug01G0337500
rosa_samantha Rh1AG344400 Rh1BG306400 Rh1CG322000 Rh1DG338600
rosa_wichuraiana Rw1G030660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1326
Acc36I ACCTGC 1 cut(s) 2917
Acc65I GGTACC 1 cut(s) 609
AccB1I GGYRCC 2 cut(s) 80, 609
AccB7I CCANNNNNTGG 3 cut(s) 664, 2955, 3145
AccBSI CCGCTC 1 cut(s) 2973
AccI GTMKAC 2 cut(s) 1653, 3013
AccII CGCG 3 cut(s) 235, 1509, 1711
AccIII TCCGGA 1 cut(s) 1962
AclWI GGATC 3 cut(s) 330, 343, 1814
AcoI YGGCCR 5 cut(s) 1438, 2623, 2800, 3037, 3190
AcsI RAATTY 3 cut(s) 18, 959, 1779
AcuI CTGAAG 6 cut(s) 650, 795, 858, 924, 1242, 2712
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 2 cut(s) 611, 2614
AfeI AGCGCT 1 cut(s) 1946
AflIII ACRYGT 1 cut(s) 2344
AjiI CACGTC 1 cut(s) 1628
AjnI CCWGG 5 cut(s) 27, 526, 1034, 1930, 3069
Alw21I GWGCWC 5 cut(s) 711, 1169, 1182, 1871, 2931
Alw26I GTCTC 7 cut(s) 145, 396, 1036, 1189, 2733, 2776, 2911
Alw44I GTGCAC 1 cut(s) 1165
AlwI GGATC 3 cut(s) 330, 343, 1814
AlwNI CAGNNNCTG 2 cut(s) 1022, 3276
Ama87I CYCGRG 2 cut(s) 238, 1316
Aor13HI TCCGGA 1 cut(s) 1962
Aor51HI AGCGCT 1 cut(s) 1946
ApaLI GTGCAC 1 cut(s) 1165
ApoI RAATTY 3 cut(s) 18, 959, 1779
ArsI GACNNNNNNTTYG 4 cut(s) 296, 328, 1646, 1678
Asp700I GAANNNNTTC 2 cut(s) 297, 1134
Asp718I GGTACC 1 cut(s) 609
AspLEI GCGC 6 cut(s) 27, 45, 83, 237, 1482, 1947
AsuC2I CCSGG 5 cut(s) 2062, 2300, 2309, 2327, 2372
AvaI CYCGRG 2 cut(s) 238, 1316
AvaII GGWCC 6 cut(s) 242, 1598, 2460, 2583, 2662, 2832
BaeGI GKGCMC 1 cut(s) 1169
BalI TGGCCA 3 cut(s) 2625, 2802, 3039
BamHI GGATCC 1 cut(s) 335
BanI GGYRCC 2 cut(s) 80, 609
BanII GRGCYC 3 cut(s) 1182, 2193, 2314
BauI CACGAG 2 cut(s) 123, 1160
BbsI GAAGAC 4 cut(s) 699, 2118, 2149, 2155
Bbv12I GWGCWC 5 cut(s) 711, 1169, 1182, 1871, 2931
BceAI ACGGC 4 cut(s) 1212, 1425, 2369, 2378
BciT130I CCWGG 5 cut(s) 29, 528, 1036, 1932, 3071
BciVI GTATCC 3 cut(s) 1516, 2712, 3168
BclI TGATCA 2 cut(s) 196, 346
BcnI CCSGG 5 cut(s) 2062, 2300, 2309, 2327, 2372
BcoDI GTCTC 7 cut(s) 145, 396, 1036, 1189, 2733, 2776, 2911
BfaI CTAG 5 cut(s) 725, 921, 935, 1638, 2723
BfmI CTRYAG 1 cut(s) 2937
BfoI RGCGCY 4 cut(s) 28, 46, 84, 1948
BfuAI ACCTGC 1 cut(s) 2917
BfuI GTATCC 3 cut(s) 1516, 2712, 3168
BglII AGATCT 3 cut(s) 914, 1354, 1747
Bme18I GGWCC 6 cut(s) 242, 1598, 2460, 2583, 2662, 2832
BmeT110I CYCGRG 2 cut(s) 238, 1316
BmgBI CACGTC 1 cut(s) 1628
BmrI ACTGGG 3 cut(s) 332, 1807, 3347
BmuI ACTGGG 3 cut(s) 332, 1807, 3347
BoxI GACNNNNGTC 2 cut(s) 247, 3242
BpiI GAAGAC 4 cut(s) 699, 2118, 2149, 2155
BpmI CTGGAG 5 cut(s) 1018, 1155, 1286, 1898, 3009
Bpu10I CCTNAGC 1 cut(s) 1847
BpuEI CTTGAG 3 cut(s) 1166, 1373, 2698
BpuMI CCSGG 5 cut(s) 2062, 2300, 2309, 2327, 2372
Bsa29I ATCGAT 2 cut(s) 440, 1809
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 2 cut(s) 145, 2733
BsaJI CCNNGG 8 cut(s) 245, 765, 803, 1441, 1709, 2060, 2298, 2586
BsaWI WCCGGW 3 cut(s) 1600, 1962, 2742
BsaXI ACNNNNNCTCC 2 cut(s) 2582, 2612
Bse118I RCCGGY 2 cut(s) 39, 3188
Bse3DI GCAATG 3 cut(s) 2872, 2949, 3225
BseAI TCCGGA 1 cut(s) 1962
BseBI CCWGG 5 cut(s) 29, 528, 1036, 1932, 3071
BseCI ATCGAT 2 cut(s) 440, 1809
BseDI CCNNGG 8 cut(s) 245, 765, 803, 1441, 1709, 2060, 2298, 2586
BseMI GCAATG 3 cut(s) 2872, 2949, 3225
BseMII CTCAG 9 cut(s) 564, 813, 1095, 1353, 1719, 2511, 2802, 2915, 3283
BseRI GAGGAG 4 cut(s) 839, 1127, 1895, 2159
BseSI GKGCMC 1 cut(s) 1169
BseYI CCCAGC 4 cut(s) 1835, 2068, 2334, 3254
BsgI GTGCAG 1 cut(s) 1446
Bsh1236I CGCG 3 cut(s) 235, 1509, 1711
Bsh1285I CGRYCG 2 cut(s) 251, 2326
BshNI GGYRCC 2 cut(s) 80, 609
BshVI ATCGAT 2 cut(s) 440, 1809
BsiEI CGRYCG 2 cut(s) 251, 2326
BsiHKAI GWGCWC 5 cut(s) 711, 1169, 1182, 1871, 2931
BsiHKCI CYCGRG 2 cut(s) 238, 1316
BslFI GGGAC 6 cut(s) 255, 419, 1611, 1776, 2659, 3354
BsmAI GTCTC 7 cut(s) 145, 396, 1036, 1189, 2733, 2776, 2911
BsmFI GGGAC 6 cut(s) 255, 419, 1611, 1776, 2659, 3354
BsmI GAATGC 4 cut(s) 374, 464, 2366, 2518
Bso31I GGTCTC 2 cut(s) 145, 2733
BsoBI CYCGRG 2 cut(s) 238, 1316
Bsp1286I GDGCHC 7 cut(s) 711, 1169, 1182, 1871, 2193, 2314, 2931
Bsp13I TCCGGA 1 cut(s) 1962
Bsp19I CCATGG 1 cut(s) 803
BspCNI CTCAG 9 cut(s) 565, 814, 1096, 1354, 1720, 2510, 2803, 2914, 3282
BspDI ATCGAT 2 cut(s) 440, 1809
BspEI TCCGGA 1 cut(s) 1962
BspFNI CGCG 3 cut(s) 235, 1509, 1711
BspHI TCATGA 1 cut(s) 199
BspMAI CTGCAG 1 cut(s) 2941
BspMI ACCTGC 1 cut(s) 2917
BspPI GGATC 3 cut(s) 330, 343, 1814
BspQI GCTCTTC 2 cut(s) 984, 1170
BspT107I GGYRCC 2 cut(s) 80, 609
BspTNI GGTCTC 2 cut(s) 145, 2733
BsrBI CCGCTC 1 cut(s) 2973
BsrDI GCAATG 3 cut(s) 2872, 2949, 3225
BsrFI RCCGGY 2 cut(s) 39, 3188
BssAI RCCGGY 2 cut(s) 39, 3188
BssECI CCNNGG 8 cut(s) 245, 765, 803, 1441, 1709, 2060, 2298, 2586
BssNAI GTATAC 1 cut(s) 3014
BssNI GRCGYC 1 cut(s) 81
BssSI CACGAG 2 cut(s) 123, 1160
BssT1I CCWWGG 3 cut(s) 765, 803, 2586
Bst1107I GTATAC 1 cut(s) 3014
Bst2BI CACGAG 2 cut(s) 123, 1160
Bst2UI CCWGG 5 cut(s) 29, 528, 1036, 1932, 3071
Bst4CI ACNGT 6 cut(s) 181, 512, 713, 888, 2541, 2621
Bst6I CTCTTC 3 cut(s) 837, 984, 1170
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 6 cut(s) 233, 1236, 1341, 1819, 2822, 3200
BstDSI CCRYGG 3 cut(s) 803, 1441, 1709
BstEII GGTNACC 1 cut(s) 2958
BstENI CCTNNNNNAGG 2 cut(s) 1061, 1846
BstFNI CGCG 3 cut(s) 235, 1509, 1711
BstH2I RGCGCY 4 cut(s) 28, 46, 84, 1948
BstHHI GCGC 6 cut(s) 27, 45, 83, 237, 1482, 1947
BstMAI GTCTC 7 cut(s) 145, 396, 1036, 1189, 2733, 2776, 2911
BstMCI CGRYCG 2 cut(s) 251, 2326
BstNI CCWGG 5 cut(s) 29, 528, 1036, 1932, 3071
BstNSI RCATGY 2 cut(s) 2348, 3202
BstPAI GACNNNNGTC 2 cut(s) 247, 3242
BstPI GGTNACC 1 cut(s) 2958
BstSFI CTRYAG 1 cut(s) 2937
BstSLI GKGCMC 1 cut(s) 1169
BstUI CGCG 3 cut(s) 235, 1509, 1711
BstV2I GAAGAC 4 cut(s) 699, 2118, 2149, 2155
BstX2I RGATCY 4 cut(s) 335, 914, 1354, 1747
BstYI RGATCY 4 cut(s) 335, 914, 1354, 1747
BstZ17I GTATAC 1 cut(s) 3014
Bsu15I ATCGAT 2 cut(s) 440, 1809
BsuI GTATCC 3 cut(s) 1516, 2712, 3168
BsuTUI ATCGAT 2 cut(s) 440, 1809
BtgI CCRYGG 3 cut(s) 803, 1441, 1709
BtrI CACGTC 1 cut(s) 1628
BtsI GCAGTG 1 cut(s) 3027
BtsIMutI CAGTG 6 cut(s) 168, 618, 709, 3027, 3246, 3335
BveI ACCTGC 1 cut(s) 2917
Cac8I GCNNGC 6 cut(s) 233, 1236, 1341, 1819, 2822, 3200
CaiI CAGNNNCTG 2 cut(s) 1022, 3276
CciI TCATGA 1 cut(s) 199
CfoI GCGC 6 cut(s) 27, 45, 83, 237, 1482, 1947
Cfr10I RCCGGY 2 cut(s) 39, 3188
Cfr42I CCGCGG 1 cut(s) 1712
ClaI ATCGAT 2 cut(s) 440, 1809
Csp6I GTAC 2 cut(s) 610, 2613
CviQI GTAC 2 cut(s) 610, 2613
DinI GGCGCC 1 cut(s) 82
DraI TTTAAA 1 cut(s) 1146
EaeI YGGCCR 5 cut(s) 1438, 2623, 2800, 3037, 3190
Eam1104I CTCTTC 3 cut(s) 837, 984, 1170
EarI CTCTTC 3 cut(s) 837, 984, 1170
EciI GGCGGA 1 cut(s) 422
Ecl136II GAGCTC 1 cut(s) 1180
Eco130I CCWWGG 3 cut(s) 765, 803, 2586
Eco147I AGGCCT 1 cut(s) 2216
Eco24I GRGCYC 3 cut(s) 1182, 2193, 2314
Eco31I GGTCTC 2 cut(s) 145, 2733
Eco47I GGWCC 6 cut(s) 242, 1598, 2460, 2583, 2662, 2832
Eco47III AGCGCT 1 cut(s) 1946
Eco53kI GAGCTC 1 cut(s) 1180
Eco57I CTGAAG 6 cut(s) 650, 795, 858, 924, 1242, 2712
Eco88I CYCGRG 2 cut(s) 238, 1316
Eco91I GGTNACC 1 cut(s) 2958
EcoICRI GAGCTC 1 cut(s) 1180
EcoNI CCTNNNNNAGG 2 cut(s) 1061, 1846
EcoO109I RGGNCCY 3 cut(s) 242, 1839, 2552
EcoO65I GGTNACC 1 cut(s) 2958
EcoRII CCWGG 5 cut(s) 27, 526, 1034, 1930, 3069
EcoT14I CCWWGG 3 cut(s) 765, 803, 2586
EcoT22I ATGCAT 2 cut(s) 2029, 2518
EcoT38I GRGCYC 3 cut(s) 1182, 2193, 2314
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
ErhI CCWWGG 3 cut(s) 765, 803, 2586
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 6 cut(s) 255, 419, 1611, 1776, 2659, 3354
FauI CCCGC 1 cut(s) 2813
FauNDI CATATG 2 cut(s) 427, 448
FbaI TGATCA 2 cut(s) 196, 346
FblI GTMKAC 2 cut(s) 1653, 3013
FriOI GRGCYC 3 cut(s) 1182, 2193, 2314
FspBI CTAG 5 cut(s) 725, 921, 935, 1638, 2723
GlaI GCGC 6 cut(s) 26, 44, 82, 236, 1481, 1946
GsaI CCCAGC 4 cut(s) 1839, 2072, 2338, 3258
GsuI CTGGAG 5 cut(s) 1018, 1155, 1286, 1898, 3009
HaeII RGCGCY 4 cut(s) 28, 46, 84, 1948
HhaI GCGC 6 cut(s) 27, 45, 83, 237, 1482, 1947
Hin1I GRCGYC 1 cut(s) 81
Hin6I GCGC 6 cut(s) 25, 43, 81, 235, 1480, 1945
HinP1I GCGC 6 cut(s) 25, 43, 81, 235, 1480, 1945
HincII GTYRAC 2 cut(s) 884, 3058
HindII GTYRAC 2 cut(s) 884, 3058
HindIII AAGCTT 1 cut(s) 2792
Hpy99I CGWCG 1 cut(s) 2399
HpyCH4III ACNGT 6 cut(s) 181, 512, 713, 888, 2541, 2621
HpyCH4IV ACGT 3 cut(s) 1303, 1627, 2115
HpySE526I ACGT 3 cut(s) 1303, 1627, 2115
Hsp92I GRCGYC 1 cut(s) 81
HspAI GCGC 6 cut(s) 25, 43, 81, 235, 1480, 1945
KasI GGCGCC 1 cut(s) 80
Kpn2I TCCGGA 1 cut(s) 1962
KpnI GGTACC 1 cut(s) 613
Ksp22I TGATCA 2 cut(s) 196, 346
KspI CCGCGG 1 cut(s) 1712
LguI GCTCTTC 2 cut(s) 984, 1170
LmnI GCTCC 7 cut(s) 706, 804, 826, 1267, 2205, 2363, 2422
MaeI CTAG 5 cut(s) 725, 921, 935, 1638, 2723
MaeII ACGT 3 cut(s) 1303, 1627, 2115
MbiI CCGCTC 1 cut(s) 2973
MfeI CAATTG 1 cut(s) 2607
MflI RGATCY 4 cut(s) 335, 914, 1354, 1747
MhlI GDGCHC 7 cut(s) 711, 1169, 1182, 1871, 2193, 2314, 2931
MlsI TGGCCA 3 cut(s) 2625, 2802, 3039
MluCI AATT 8 cut(s) 18, 959, 1619, 1779, 2132, 2251, 2607, 3141
MluNI TGGCCA 3 cut(s) 2625, 2802, 3039
Mly113I GGCGCC 1 cut(s) 81
MlyI GAGTC 5 cut(s) 441, 1380, 1708, 2641, 2778
MmeI TCCRAC 5 cut(s) 572, 2039, 2464, 2859, 3329
Mox20I TGGCCA 3 cut(s) 2625, 2802, 3039
Mph1103I ATGCAT 2 cut(s) 2029, 2518
MroI TCCGGA 1 cut(s) 1962
MroXI GAANNNNTTC 2 cut(s) 297, 1134
MscI TGGCCA 3 cut(s) 2625, 2802, 3039
MseI TTAA 5 cut(s) 648, 968, 1145, 1454, 1907
Msp20I TGGCCA 3 cut(s) 2625, 2802, 3039
MspA1I CMGCKG 3 cut(s) 1711, 1770, 2936
MunI CAATTG 1 cut(s) 2607
Mva1269I GAATGC 4 cut(s) 374, 464, 2366, 2518
MvaI CCWGG 5 cut(s) 29, 528, 1036, 1932, 3071
MvnI CGCG 3 cut(s) 235, 1509, 1711
NarI GGCGCC 1 cut(s) 81
NciI CCSGG 5 cut(s) 2062, 2300, 2309, 2327, 2372
NcoI CCATGG 1 cut(s) 803
NdeI CATATG 2 cut(s) 427, 448
NmeAIII GCCGAG 1 cut(s) 2762
NmuCI GTSAC 9 cut(s) 158, 166, 181, 2530, 2543, 2958, 3222, 3244, 3333
NsiI ATGCAT 2 cut(s) 2029, 2518
NspI RCATGY 2 cut(s) 2348, 3202
PaeI GCATGC 1 cut(s) 3202
PagI TCATGA 1 cut(s) 199
PceI AGGCCT 1 cut(s) 2216
PciI ACATGT 1 cut(s) 2344
PciSI GCTCTTC 2 cut(s) 984, 1170
PctI GAATGC 4 cut(s) 374, 464, 2366, 2518
PdmI GAANNNNTTC 2 cut(s) 297, 1134
PfeI GAWTC 8 cut(s) 944, 1049, 1959, 2011, 2283, 2330, 2446, 3273
PflFI GACNNNGTC 2 cut(s) 156, 2117
PflMI CCANNNNNTGG 3 cut(s) 664, 2955, 3145
PfoI TCCNGGA 3 cut(s) 526, 1034, 1930
PleI GAGTC 5 cut(s) 440, 1380, 1708, 2641, 2777
PluTI GGCGCC 1 cut(s) 84
PpsI GAGTC 5 cut(s) 440, 1380, 1708, 2641, 2777
PpuMI RGGWCCY 1 cut(s) 242
PscI ACATGT 1 cut(s) 2344
PshAI GACNNNNGTC 2 cut(s) 247, 3242
PsiI TTATAA 1 cut(s) 1326
Psp124BI GAGCTC 1 cut(s) 1182
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 5 cut(s) 27, 526, 1034, 1930, 3069
PspEI GGTNACC 1 cut(s) 2958
PspFI CCCAGC 4 cut(s) 1835, 2068, 2334, 3254
PspGI CCWGG 5 cut(s) 27, 526, 1034, 1930, 3069
PspPPI RGGWCCY 1 cut(s) 242
PstI CTGCAG 1 cut(s) 2941
PstNI CAGNNNCTG 2 cut(s) 1022, 3276
PsuI RGATCY 4 cut(s) 335, 914, 1354, 1747
PsyI GACNNNGTC 2 cut(s) 156, 2117
PvuII CAGCTG 1 cut(s) 2936
RsaI GTAC 2 cut(s) 611, 2614
RsaNI GTAC 2 cut(s) 610, 2613
SacI GAGCTC 1 cut(s) 1182
SacII CCGCGG 1 cut(s) 1712
SapI GCTCTTC 2 cut(s) 984, 1170
SaqAI TTAA 5 cut(s) 648, 968, 1145, 1454, 1907
SchI GAGTC 5 cut(s) 441, 1380, 1708, 2641, 2778
SduI GDGCHC 7 cut(s) 711, 1169, 1182, 1871, 2193, 2314, 2931
SfcI CTRYAG 1 cut(s) 2937
SfoI GGCGCC 1 cut(s) 82
Sfr303I CCGCGG 1 cut(s) 1712
SgrBI CCGCGG 1 cut(s) 1712
SinI GGWCC 6 cut(s) 242, 1598, 2460, 2583, 2662, 2832
SmlI CTYRAG 3 cut(s) 1181, 1388, 2713
SmoI CTYRAG 3 cut(s) 1181, 1388, 2713
SphI GCATGC 1 cut(s) 3202
Sse9I AATT 8 cut(s) 18, 959, 1619, 1779, 2132, 2251, 2607, 3141
SseBI AGGCCT 1 cut(s) 2216
SspDI GGCGCC 1 cut(s) 80
SspI AATATT 1 cut(s) 2096
SspMI CTAG 5 cut(s) 725, 921, 935, 1638, 2723
SstI GAGCTC 1 cut(s) 1182
StuI AGGCCT 1 cut(s) 2216
StyI CCWWGG 3 cut(s) 765, 803, 2586
TaaI ACNGT 6 cut(s) 181, 512, 713, 888, 2541, 2621
TaiI ACGT 3 cut(s) 1306, 1630, 2118
TaqII GACCGA 1 cut(s) 237
TasI AATT 8 cut(s) 18, 959, 1619, 1779, 2132, 2251, 2607, 3141
TauI GCSGC 2 cut(s) 1512, 2976
TfiI GAWTC 8 cut(s) 944, 1049, 1959, 2011, 2283, 2330, 2446, 3273
Tru1I TTAA 5 cut(s) 648, 968, 1145, 1454, 1907
Tru9I TTAA 5 cut(s) 648, 968, 1145, 1454, 1907
TscAI CASTG 6 cut(s) 175, 625, 716, 3034, 3253, 3342
TseFI GTSAC 9 cut(s) 158, 166, 181, 2530, 2543, 2958, 3222, 3244, 3333
Tsp45I GTSAC 9 cut(s) 158, 166, 181, 2530, 2543, 2958, 3222, 3244, 3333
TspDTI ATGAA 8 cut(s) 945, 957, 1127, 1202, 1269, 1557, 1626, 3276
TspGWI ACGGA 2 cut(s) 543, 2376
TspRI CASTG 6 cut(s) 175, 625, 716, 3034, 3253, 3342
Tth111I GACNNNGTC 2 cut(s) 156, 2117
Van91I CCANNNNNTGG 3 cut(s) 664, 2955, 3145
VneI GTGCAC 1 cut(s) 1165
VpaK11BI GGWCC 6 cut(s) 242, 1598, 2460, 2583, 2662, 2832
XagI CCTNNNNNAGG 2 cut(s) 1061, 1846
XapI RAATTY 3 cut(s) 18, 959, 1779
XbaI TCTAGA 1 cut(s) 724
XceI RCATGY 2 cut(s) 2348, 3202
XmiI GTMKAC 2 cut(s) 1653, 3013
XmnI GAANNNNTTC 2 cut(s) 297, 1134
XspI CTAG 5 cut(s) 725, 921, 935, 1638, 2723
Zsp2I ATGCAT 2 cut(s) 2029, 2518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.