Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0018106

Physical Location & Seq

Genomic Coordinates Reverse (-)

25641266 .. 25643228

Gene Structure 1963 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1147400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 417 bp

ATGCAGGAAAATAAATCAGACTCGCCCAACAAAAACCCTAAGAAGGCCAATCTCCTCGACCACCACTCCATCAAACACACCCTCGACGAGTCCGTCACCGAGATCGTGACGGGCCGCGGATATGTGGAGGACGTGCGGCTGAGCAATGTGAGGCTGTTTCTGGGCACCATCATCATCGCCATCGCTCTCTTCGCTCAGTTTTACAAGAAGAAGTTCCCCGAAAACCGAGATTTTCTCATCCTCTGCATCCTTGAAGTGTGTTTGATCTTTGACTCTTGCACGTATATAGTCTTCAATGCGGTGTTGCAGCTCATCATATTCACCAAGGAGAAGAATGCCATTCTCTTTACGTATCCTCCCGTGGGAGCTTTTGTGTTTTCTTCTCTGGTTGACGCAACTGCTTACCCCTGTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005787 GO:0005789 GO:0006465 GO:0006508 GO:0006518 GO:0006605 GO:0006807 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016485 GO:0019538 GO:0031984 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034641 GO:0042175 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070727 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0098796 GO:0098827 GO:1901564 GO:1902494 GO:1905368

KEGG Pathways

Metabolic & Signaling

ko03060 map03060

Pfam Domains

Protein Families

SPC25

Protein Analysis

139

Amino Acids

15.65

Weight (kDa)

6.81

Isoelectric Point (pI)

41.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPC25	PF06703	24 - 121	1.3e-20	Microsomal signal peptidase 25 kDa subunit (SPC25)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018106)

Species	Orthologous Gene IDs
malus_domestica	MD01G1147400.v1.1 MD08G1058400.v1.1
prunus_persica	Prupe.2G246300_v2.0.a1
pyrus_communis	pycom07g19680
rosa_chinensis	RchiOBHm_Chr1g0369421
rosa_multiflora	Rmu_sc0006332.1_g000002
rosa_rugosa	Rorug01G0350000
rosa_samantha	Rh1AG357600 Rh1BG321700 Rh1DG352300 Rh6CG416000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	92
AccB1I	GGYRCC	1 cut(s)	164
AccII	CGCG	1 cut(s)	117
AciI	CCGC	4 cut(s)	115, 117, 136, 299
AfiI	CCNNNNNNNGG	2 cut(s)	43, 362
AgsI	TTSAA	2 cut(s)	254, 295
AjiI	CACGTC	1 cut(s)	133
AluBI	AGCT	2 cut(s)	310, 368
AluI	AGCT	2 cut(s)	310, 368
AoxI	GGCC	2 cut(s)	45, 112
ApeKI	GCWGC	1 cut(s)	307
Asp700I	GAANNNNTTC	1 cut(s)	212
AspS9I	GGNCC	1 cut(s)	112
AsuHPI	GGTGA	2 cut(s)	88, 313
BaeGI	GKGCMC	1 cut(s)	167
BanI	GGYRCC	1 cut(s)	164
BarI	GAAGNNNNNNTAC	2 cut(s)	275, 307
BbsI	GAAGAC	1 cut(s)	283
BbvI	GCAGC	1 cut(s)	319
BccI	CCATC	3 cut(s)	77, 176, 188
BciVI	GTATCC	1 cut(s)	363
BfuI	GTATCC	1 cut(s)	363
BisI	GCNGC	3 cut(s)	115, 137, 308
BlpI	GCTNAGC	1 cut(s)	140
BlsI	GCNGC	3 cut(s)	116, 138, 309
BmgBI	CACGTC	1 cut(s)	133
BmgT120I	GGNCC	1 cut(s)	112
BmiI	GGNNCC	1 cut(s)	166
BmsI	GCATC	1 cut(s)	255
BpiI	GAAGAC	1 cut(s)	283
BplI	GAGNNNNNCTC	2 cut(s)	219, 251
Bpu1102I	GCTNAGC	1 cut(s)	140
BsaAI	YACGTR	2 cut(s)	282, 351
BsaJI	CCNNGG	3 cut(s)	115, 324, 360
BsaXI	ACNNNNNCTCC	6 cut(s)	50, 80, 340, 357, 370, 387
Bsc4I	CCNNNNNNNGG	2 cut(s)	43, 362
Bse3DI	GCAATG	1 cut(s)	151
BseDI	CCNNGG	3 cut(s)	115, 324, 360
BseGI	GGATG	2 cut(s)	237, 246
BseLI	CCNNNNNNNGG	2 cut(s)	43, 362
BseMI	GCAATG	1 cut(s)	151
BseMII	CTCAG	2 cut(s)	131, 209
BseRI	GAGGAG	1 cut(s)	44
BseSI	GKGCMC	1 cut(s)	167
BseXI	GCAGC	1 cut(s)	319
Bsh1236I	CGCG	1 cut(s)	117
BshFI	GGCC	2 cut(s)	47, 114
BshNI	GGYRCC	1 cut(s)	164
BslI	CCNNNNNNNGG	2 cut(s)	43, 362
BsmI	GAATGC	1 cut(s)	340
BsnI	GGCC	2 cut(s)	47, 114
Bsp1286I	GDGCHC	1 cut(s)	167
Bsp143I	GATC	2 cut(s)	102, 264
Bsp1720I	GCTNAGC	1 cut(s)	140
BspACI	CCGC	4 cut(s)	115, 117, 136, 299
BspANI	GGCC	2 cut(s)	47, 114
BspCNI	CTCAG	2 cut(s)	132, 208
BspFNI	CGCG	1 cut(s)	117
BspLI	GGNNCC	1 cut(s)	166
BspT107I	GGYRCC	1 cut(s)	164
BsrDI	GCAATG	1 cut(s)	151
BssECI	CCNNGG	3 cut(s)	115, 324, 360
BssMI	GATC	2 cut(s)	102, 264
BssT1I	CCWWGG	1 cut(s)	324
Bst6I	CTCTTC	1 cut(s)	194
BstBAI	YACGTR	2 cut(s)	282, 351
BstDEI	CTNAG	3 cut(s)	39, 140, 195
BstDSI	CCRYGG	2 cut(s)	115, 360
BstF5I	GGATG	2 cut(s)	237, 246
BstFNI	CGCG	1 cut(s)	117
BstKTI	GATC	2 cut(s)	105, 267
BstMBI	GATC	2 cut(s)	102, 264
BstMWI	GCNNNNNNNGC	1 cut(s)	191
BstSLI	GKGCMC	1 cut(s)	167
BstSNI	TACGTA	1 cut(s)	351
BstUI	CGCG	1 cut(s)	117
BstV1I	GCAGC	1 cut(s)	319
BstV2I	GAAGAC	1 cut(s)	283
BsuI	GTATCC	1 cut(s)	363
BsuRI	GGCC	2 cut(s)	47, 114
BtgI	CCRYGG	2 cut(s)	115, 360
BtgZI	GCGATG	2 cut(s)	160, 166
BtrI	CACGTC	1 cut(s)	133
BtsCI	GGATG	2 cut(s)	237, 246
Cfr13I	GGNCC	1 cut(s)	112
Cfr42I	CCGCGG	1 cut(s)	118
CseI	GACGC	1 cut(s)	401
CviJI	RGCY	6 cut(s)	47, 114, 139, 154, 310, 368
CviKI_1	RGCY	6 cut(s)	47, 114, 139, 154, 310, 368
DdeI	CTNAG	3 cut(s)	39, 140, 195
DpnI	GATC	2 cut(s)	104, 266
DpnII	GATC	2 cut(s)	102, 264
DrdI	GACNNNNNNGTC	1 cut(s)	92
DseDI	GACNNNNNNGTC	1 cut(s)	92
Eam1104I	CTCTTC	1 cut(s)	194
EarI	CTCTTC	1 cut(s)	194
Eco105I	TACGTA	1 cut(s)	351
Eco130I	CCWWGG	1 cut(s)	324
EcoT14I	CCWWGG	1 cut(s)	324
ErhI	CCWWGG	1 cut(s)	324
FaiI	YATR	4 cut(s)	123, 285, 287, 317
Fnu4HI	GCNGC	3 cut(s)	115, 137, 308
FokI	GGATG	2 cut(s)	224, 233
Fsp4HI	GCNGC	3 cut(s)	115, 137, 308
GluI	GCNGC	3 cut(s)	115, 137, 308
HaeIII	GGCC	2 cut(s)	47, 114
HgaI	GACGC	1 cut(s)	401
HincII	GTYRAC	1 cut(s)	391
HindII	GTYRAC	1 cut(s)	391
HinfI	GANTC	3 cut(s)	20, 89, 272
HphI	GGTGA	2 cut(s)	88, 313
Hpy166II	GTNNAC	1 cut(s)	391
Hpy188I	TCNGA	1 cut(s)	19
Hpy188III	TCNNGA	1 cut(s)	106
Hpy8I	GTNNAC	1 cut(s)	391
Hpy99I	CGWCG	1 cut(s)	89
HpyAV	CCTTC	1 cut(s)	37
HpyCH4IV	ACGT	3 cut(s)	132, 281, 350
HpyCH4V	TGCA	4 cut(s)	4, 246, 279, 307
HpyF10VI	GCNNNNNNNGC	1 cut(s)	191
HpyF3I	CTNAG	3 cut(s)	39, 140, 195
HpySE526I	ACGT	3 cut(s)	132, 281, 350
KspI	CCGCGG	1 cut(s)	118
Kzo9I	GATC	2 cut(s)	102, 264
LmnI	GCTCC	1 cut(s)	365
LpnPI	CCDG	2 cut(s)	146, 371
Lsp1109I	GCAGC	1 cut(s)	319
LweI	GCATC	1 cut(s)	255
MaeII	ACGT	3 cut(s)	132, 281, 350
MaeIII	GTNAC	2 cut(s)	94, 106
MalI	GATC	2 cut(s)	104, 266
MboI	GATC	2 cut(s)	102, 264
MboII	GAAGA	5 cut(s)	181, 220, 283, 343, 372
MhlI	GDGCHC	1 cut(s)	167
MlyI	GAGTC	3 cut(s)	14, 98, 266
MnlI	CCTC	6 cut(s)	65, 92, 121, 144, 251, 366
MroXI	GAANNNNTTC	1 cut(s)	212
MspA1I	CMGCKG	1 cut(s)	117
Mva1269I	GAATGC	1 cut(s)	340
MvnI	CGCG	1 cut(s)	117
MwoI	GCNNNNNNNGC	1 cut(s)	191
NdeII	GATC	2 cut(s)	102, 264
NlaIV	GGNNCC	1 cut(s)	166
NmuCI	GTSAC	2 cut(s)	94, 106
PcsI	WCGNNNNNNNCGW	1 cut(s)	90
PctI	GAATGC	1 cut(s)	340
PdmI	GAANNNNTTC	1 cut(s)	212
PkrI	GCNGC	3 cut(s)	116, 138, 309
PleI	GAGTC	3 cut(s)	14, 97, 266
PpsI	GAGTC	3 cut(s)	14, 97, 266
Ppu21I	YACGTR	2 cut(s)	282, 351
PspN4I	GGNNCC	1 cut(s)	166
PspPI	GGNCC	1 cut(s)	112
SacII	CCGCGG	1 cut(s)	118
SatI	GCNGC	3 cut(s)	115, 137, 308
Sau3AI	GATC	2 cut(s)	102, 264
Sau96I	GGNCC	1 cut(s)	112
SchI	GAGTC	3 cut(s)	14, 98, 266
SduI	GDGCHC	1 cut(s)	167
SetI	ASST	5 cut(s)	135, 284, 312, 353, 370
SfaNI	GCATC	1 cut(s)	255
Sfr303I	CCGCGG	1 cut(s)	118
SgrBI	CCGCGG	1 cut(s)	118
SnaBI	TACGTA	1 cut(s)	351
SsiI	CCGC	4 cut(s)	115, 117, 136, 299
StyI	CCWWGG	1 cut(s)	324
TaiI	ACGT	3 cut(s)	135, 284, 353
TaqI	TCGA	2 cut(s)	57, 84
TauI	GCSGC	2 cut(s)	117, 139
TseFI	GTSAC	2 cut(s)	94, 106
TseI	GCWGC	1 cut(s)	307
Tsp45I	GTSAC	2 cut(s)	94, 106
TspGWI	ACGGA	1 cut(s)	82
XmnI	GAANNNNTTC	1 cut(s)	212

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.