Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0018106

Physical Location & Seq

Genomic Coordinates Reverse (-)

4658576 .. 4659241

Gene Structure 666 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1058400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 276 bp

ATGTCGGAGACGAAGGCCGAGAGCAAGAGCAAAAACCCTAAGAAGGTCAATCTCTCAGATCAGAGCTCCATCAAGCACCTCCTCGACGAGTCCGTCTCCGAGATTGTTAAGAGTCGCGGGTATGTGGAGAATACGAGGCTGAGTAACTTGAAGTTGTTGATTGGAACCGTTGTGATAATCATTGCTTTGTTTGCTCAGTTCTACAACAAGAAATTTCCGGAGAACCGAGATTTCCTGCTTGGATGCATTGCATTATATCCTTTTTCTTTGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005787 GO:0005789 GO:0006465 GO:0006508 GO:0006518 GO:0006605 GO:0006807 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016485 GO:0019538 GO:0031984 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034641 GO:0042175 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070727 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0098796 GO:0098827 GO:1901564 GO:1902494 GO:1905368

KEGG Pathways

Metabolic & Signaling

ko03060 map03060

Pfam Domains

Protein Families

SPC25

Protein Analysis

92

Amino Acids

10.29

Weight (kDa)

9.34

Isoelectric Point (pI)

37.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPC25	PF06703	24 - 86	8e-16	Microsomal signal peptidase 25 kDa subunit (SPC25)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018106)

Species	Orthologous Gene IDs
malus_domestica	MD01G1147400.v1.1 MD08G1058400.v1.1
prunus_persica	Prupe.2G246300_v2.0.a1
pyrus_communis	pycom07g19680
rosa_chinensis	RchiOBHm_Chr1g0369421
rosa_multiflora	Rmu_sc0006332.1_g000002
rosa_rugosa	Rorug01G0350000
rosa_samantha	Rh1AG357600 Rh1BG321700 Rh1DG352300 Rh6CG416000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	92
AccII	CGCG	1 cut(s)	117
AccIII	TCCGGA	1 cut(s)	217
AciI	CCGC	1 cut(s)	117
AcsI	RAATTY	1 cut(s)	212
AfiI	CCNNNNNNNGG	1 cut(s)	43
AgsI	TTSAA	2 cut(s)	151, 271
AluBI	AGCT	1 cut(s)	66
AluI	AGCT	1 cut(s)	66
Alw21I	GWGCWC	1 cut(s)	68
Alw26I	GTCTC	2 cut(s)	2, 100
Aor13HI	TCCGGA	1 cut(s)	217
AoxI	GGCC	1 cut(s)	15
ApoI	RAATTY	1 cut(s)	212
BanII	GRGCYC	1 cut(s)	68
Bbv12I	GWGCWC	1 cut(s)	68
BccI	CCATC	1 cut(s)	77
BcoDI	GTCTC	2 cut(s)	2, 100
BmiI	GGNNCC	1 cut(s)	166
BmsI	GCATC	1 cut(s)	233
BplI	GAGNNNNNCTC	2 cut(s)	80, 112
BsaWI	WCCGGW	1 cut(s)	217
Bsc4I	CCNNNNNNNGG	1 cut(s)	43
Bse3DI	GCAATG	2 cut(s)	180, 246
BseAI	TCCGGA	1 cut(s)	217
BseGI	GGATG	1 cut(s)	248
BseLI	CCNNNNNNNGG	1 cut(s)	43
BseMI	GCAATG	2 cut(s)	180, 246
BseMII	CTCAG	3 cut(s)	69, 131, 209
BseRI	GAGGAG	1 cut(s)	71
Bsh1236I	CGCG	1 cut(s)	117
BshFI	GGCC	1 cut(s)	17
BsiHKAI	GWGCWC	1 cut(s)	68
BsiSI	CCGG	1 cut(s)	218
BslI	CCNNNNNNNGG	1 cut(s)	43
BsmAI	GTCTC	2 cut(s)	2, 100
BsmBI	CGTCTC	2 cut(s)	2, 100
BsnI	GGCC	1 cut(s)	17
Bsp1286I	GDGCHC	1 cut(s)	68
Bsp13I	TCCGGA	1 cut(s)	217
Bsp143I	GATC	1 cut(s)	58
BspACI	CCGC	1 cut(s)	117
BspANI	GGCC	1 cut(s)	17
BspCNI	CTCAG	3 cut(s)	68, 132, 208
BspEI	TCCGGA	1 cut(s)	217
BspFNI	CGCG	1 cut(s)	117
BspLI	GGNNCC	1 cut(s)	166
BsrDI	GCAATG	2 cut(s)	180, 246
BssMI	GATC	1 cut(s)	58
Bst4CI	ACNGT	1 cut(s)	169
BstDEI	CTNAG	4 cut(s)	39, 55, 140, 195
BstF5I	GGATG	1 cut(s)	248
BstFNI	CGCG	1 cut(s)	117
BstKTI	GATC	1 cut(s)	61
BstMAI	GTCTC	2 cut(s)	2, 100
BstMBI	GATC	1 cut(s)	58
BstMWI	GCNNNNNNNGC	1 cut(s)	191
BstUI	CGCG	1 cut(s)	117
BsuRI	GGCC	1 cut(s)	17
BtsCI	GGATG	1 cut(s)	248
CviJI	RGCY	3 cut(s)	17, 66, 139
CviKI_1	RGCY	3 cut(s)	17, 66, 139
DdeI	CTNAG	4 cut(s)	39, 55, 140, 195
DpnI	GATC	1 cut(s)	60
DpnII	GATC	1 cut(s)	58
DrdI	GACNNNNNNGTC	1 cut(s)	92
DseDI	GACNNNNNNGTC	1 cut(s)	92
Ecl136II	GAGCTC	1 cut(s)	66
Eco24I	GRGCYC	1 cut(s)	68
Eco53kI	GAGCTC	1 cut(s)	66
EcoICRI	GAGCTC	1 cut(s)	66
EcoT22I	ATGCAT	1 cut(s)	248
EcoT38I	GRGCYC	1 cut(s)	68
Esp3I	CGTCTC	2 cut(s)	2, 100
FaiI	YATR	2 cut(s)	123, 256
FauI	CCCGC	1 cut(s)	110
FokI	GGATG	1 cut(s)	255
FriOI	GRGCYC	1 cut(s)	68
HaeIII	GGCC	1 cut(s)	17
HapII	CCGG	1 cut(s)	218
HinfI	GANTC	2 cut(s)	89, 112
HpaII	CCGG	1 cut(s)	218
Hpy188I	TCNGA	4 cut(s)	7, 58, 63, 100
Hpy188III	TCNNGA	1 cut(s)	218
Hpy99I	CGWCG	1 cut(s)	89
HpyAV	CCTTC	2 cut(s)	7, 37
HpyCH4III	ACNGT	1 cut(s)	169
HpyCH4V	TGCA	2 cut(s)	246, 251
HpyF10VI	GCNNNNNNNGC	1 cut(s)	191
HpyF3I	CTNAG	4 cut(s)	39, 55, 140, 195
Kpn2I	TCCGGA	1 cut(s)	217
Kzo9I	GATC	1 cut(s)	58
LmnI	GCTCC	1 cut(s)	71
LpnPI	CCDG	2 cut(s)	231, 248
LweI	GCATC	1 cut(s)	233
MaeIII	GTNAC	1 cut(s)	143
MalI	GATC	1 cut(s)	60
MboI	GATC	1 cut(s)	58
MhlI	GDGCHC	1 cut(s)	68
MluCI	AATT	2 cut(s)	212, 271
MlyI	GAGTC	2 cut(s)	98, 121
MnlI	CCTC	3 cut(s)	89, 92, 129
Mph1103I	ATGCAT	1 cut(s)	248
MroI	TCCGGA	1 cut(s)	217
MseI	TTAA	1 cut(s)	108
MspI	CCGG	1 cut(s)	218
MvnI	CGCG	1 cut(s)	117
MwoI	GCNNNNNNNGC	1 cut(s)	191
NdeII	GATC	1 cut(s)	58
NlaIV	GGNNCC	1 cut(s)	166
NmeAIII	GCCGAG	1 cut(s)	43
NsiI	ATGCAT	1 cut(s)	248
PcsI	WCGNNNNNNNCGW	1 cut(s)	90
PleI	GAGTC	2 cut(s)	97, 120
PpsI	GAGTC	2 cut(s)	97, 120
Psp124BI	GAGCTC	1 cut(s)	68
PspN4I	GGNNCC	1 cut(s)	166
SacI	GAGCTC	1 cut(s)	68
SaqAI	TTAA	1 cut(s)	108
Sau3AI	GATC	1 cut(s)	58
SchI	GAGTC	2 cut(s)	98, 121
SduI	GDGCHC	1 cut(s)	68
SetI	ASST	3 cut(s)	48, 68, 81
SfaNI	GCATC	1 cut(s)	233
Sse9I	AATT	2 cut(s)	212, 271
SsiI	CCGC	1 cut(s)	117
SstI	GAGCTC	1 cut(s)	68
TaaI	ACNGT	1 cut(s)	169
TaqI	TCGA	1 cut(s)	84
TasI	AATT	2 cut(s)	212, 271
Tru1I	TTAA	1 cut(s)	108
Tru9I	TTAA	1 cut(s)	108
TspGWI	ACGGA	1 cut(s)	82
XapI	RAATTY	1 cut(s)	212
Zsp2I	ATGCAT	1 cut(s)	248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.