Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009798

Physical Location & Seq

Genomic Coordinates Forward (+)

26130364 .. 26134218

Gene Structure 3855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1153000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1779 bp

ATGTCGAAGCAGACCTACAAAGTGTGCTTCTGCTTCCAGCGGCGCTTCAAGCTCGCCGTCAAGGAAGCACCGCCGGAGATCAAGGCCTTGTTCGATCAATACTCGGAGAACGGCATCATGACCGCCGATCACTTCCACCGATTCCTGACGGAAGTGCAGAAGGAGGAGAGCGCCACCAAGGAGGAAGCTCAGGCCCTCATCGAACAGAGCCTCTCCGAGCTCAAACACCTCAACATCTTCCACCGCAAGGTCCTCAATCTCGAAGCATTCTTCAAGTACCTCTTCGGCGACATCAATCCCCCAGTCTCTCCAGCTCTCGGGGTGCACCATGATATGAATGCGCCATTGTCGCATTATTTCATATACACAGGGCACAATTCGTATCTGACTGGGAACCAGCTGAGCAGTGACTGCAGTGACGTTCCTATTATTCAAGCGCTTCACAGAGGAGTGAGGGTGATTGAGCTGGATATTTGGCCGAATTCGAATAAAGACGATGTCGATGTGCTTCATGGAGGGACATTGACTACCCCGGTGGAACTTATCAAGTGTTTGAAGTCTATTAAGGAACATGCCTTTGTTGCCTCTGAATACCCTGTTGTAATCACTTTGGAAGACCACCTGACCCCGGATCTTCAAACTAAAGTCGCTAAGATGGTCACTGAAACATTTGGAGAGGTACTGTTTTCTCCTGGCTCGGAATGTCTGAAAGAATTTCCATCCCCAGCGTCACTAAAGAAAAGGATTATTATCTCGACCAAACCGCCTCAGGAATATCGTGAGGCAAAGGATGCTCTGCAAAGTGAAAGTGATCCGAAGAGTGGAAAACCTCCAGCTGATGAGGAAGCGTGGGGTAAAGAAGTCCCGGACGCCAAAGGTGCAATTGCGGTAGATGACAAGCACGATGAATTGGATGAAGAAGATAGCAATGAGGAGGAAGATGTTGATAATGGTGGTTCCAAGTCCGTTGCACCTGAATATAAGCGTTTAATTGCCATTCATGCTGGGAAACCCAAAGGTGGATTAGCAGAGTGTCTCAAAGTAGATCCCAATAAAGTGCGGCGTCTTAGCTTAAGTGAGCAACAGCTCGAGAAGGCAGCAGTAACCTATGGAAAGGAAATTGTCAGGTTTACTCAGAGGAATATTTTGAGGGTGTACCCAAAGGGCATACGTGTTGACTCATCAAATTATAACCCAATGATTGGGTGGTCGCATGGGGCTCAAATGGTTGCATTCAATATGCAGGGATATGGAAGATCATTGTGGTTGATGCAGGGAATGTTTAGAGCCAATGGTGGGTGCGGCTATGTGAAAAAACCGGATTTTCTTTTGAAGAGTGGTCCACATAATGAGGTCTTTGATCCTAAATCTGATTTACCAGTGAAACAAACTTTGAAGGTAAAAGTATATATGGGAGAAGGATGGTACTATGATTTCAAGCATACACACTTCGATGCATATTCTCCTCCAGATTTTTATGCAAGGGTAGGAATTGCTGGAGTTGCTGCTGATAGTGTGATGAAGAAGACAAAGACCCTAGAGGACGACTGGAGTCCAGCGTGGAACGAAGAATTTGCATTCCCATTAACTGTTCCAGAACTAGCTGTGCTCCGCGTTGAAGTTCACGAGTACGACATGTCAGAAAAGGACGATTTCGGAGGCCAAACATGCCTACCGGTGTCTGAGCTCAGAACAGGGATTCGAGCAGTTCCATTAAACTGCCGGAAGGGAGTGCCGTACAAATCCGTAAAGCTTCTCATGCACTTTGATTTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004629 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007049 GO:0007275 GO:0008081 GO:0008150 GO:0009553 GO:0009555 GO:0009556 GO:0009605 GO:0009607 GO:0009617 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010600 GO:0010601 GO:0010817 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0034293 GO:0042578 GO:0042742 GO:0043207 GO:0043934 GO:0044464 GO:0044703 GO:0046885 GO:0046886 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051707 GO:0061458 GO:0065007 GO:0065008 GO:0071944 GO:0090354 GO:0090355 GO:0090567 GO:0098542 GO:0099402 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933

Pfam Domains

Protein Families

C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

593

Amino Acids

66.67

Weight (kDa)

5.9

Isoelectric Point (pI)

47.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_like	PF09279	26 - 94	1.8e-06	Phosphoinositide-specific phospholipase C, efhand-like
PI-PLC-X	PF00388	112 - 254	1.2e-52	Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y	PF00387	356 - 442	1.4e-29	Phosphatidylinositol-specific phospholipase C, Y domain
C2	PF00168	464 - 565	3.2e-13	C2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009798)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08510 AT3G08510 AT3G08510 AT3G08510 AT3G55940
fragaria_vesca	FvH4_7g24471 FvH4_7g24471
malus_domestica	MD01G1153000.v1.1 MD07G1221900.v1.1
prunus_persica	Prupe.2G250100_v2.0.a1
pyrus_communis	pycom01g17060 pycom07g20040
rosa_chinensis	RchiOBHm_Chr1g0370231
rosa_laevigata	RLG00000027067
rosa_multiflora	Rmu_sc0017405.1_g000001 Rmu_sc0030456.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286730
rosa_rugosa	Rorug01G0354700 Rorug01G0354800
rosa_samantha	Rh1AG363500
rosa_wichuraiana	Rw1G031990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1191
AccB7I	CCANNNNNTGG	1 cut(s)	1202
AccII	CGCG	1 cut(s)	1614
AciI	CCGC	9 cut(s)	40, 71, 123, 244, 764, 887, 1060, 1302, 1612
AclWI	GGATC	4 cut(s)	639, 806, 1040, 1355
AcoI	YGGCCR	1 cut(s)	476
AcsI	RAATTY	3 cut(s)	481, 713, 1571
AcyI	GRCGYC	2 cut(s)	870, 1063
AfaI	GTAC	6 cut(s)	278, 681, 1157, 1427, 1631, 1739
AfeI	AGCGCT	1 cut(s)	438
AfiI	CCNNNNNNNGG	7 cut(s)	247, 317, 628, 821, 1019, 1202, 1296
AflII	CTTAAG	1 cut(s)	1072
AflIII	ACRYGT	2 cut(s)	1171, 1635
AgeI	ACCGGT	1 cut(s)	1675
AjnI	CCWGG	1 cut(s)	691
Alw21I	GWGCWC	4 cut(s)	222, 327, 1611, 1689
Alw26I	GTCTC	2 cut(s)	310, 1040
Alw44I	GTGCAC	1 cut(s)	323
AlwI	GGATC	4 cut(s)	639, 806, 1040, 1355
AlwNI	CAGNNNCTG	1 cut(s)	411
Ama87I	CYCGRG	2 cut(s)	317, 1088
Aor51HI	AGCGCT	1 cut(s)	438
AoxI	GGCC	4 cut(s)	84, 192, 476, 1660
ApaLI	GTGCAC	1 cut(s)	323
ApeKI	GCWGC	2 cut(s)	1097, 1505
ApoI	RAATTY	3 cut(s)	481, 713, 1571
AsiGI	ACCGGT	1 cut(s)	1675
AspLEI	GCGC	4 cut(s)	45, 173, 343, 439
AspS9I	GGNCC	3 cut(s)	193, 250, 1340
AsuC2I	CCSGG	3 cut(s)	533, 629, 866
AsuHPI	GGTGA	1 cut(s)	469
AsuII	TTCGAA	1 cut(s)	485
AvaI	CYCGRG	2 cut(s)	317, 1088
AvaII	GGWCC	2 cut(s)	250, 1340
AxyI	CCTNAGG	1 cut(s)	768
BaeGI	GKGCMC	2 cut(s)	327, 375
BanII	GRGCYC	3 cut(s)	222, 1222, 1689
BarI	GAAGNNNNNNTAC	1 cut(s)	31
BauI	CACGAG	1 cut(s)	1625
BbsI	GAAGAC	2 cut(s)	621, 1532
Bbv12I	GWGCWC	4 cut(s)	222, 327, 1611, 1689
BbvI	GCAGC	2 cut(s)	1109, 1492
BccI	CCATC	3 cut(s)	649, 727, 1416
BceAI	ACGGC	3 cut(s)	41, 127, 1720
BcgI	CGANNNNNNTGC	2 cut(s)	1556, 1590
BciT130I	CCWGG	1 cut(s)	693
BcnI	CCSGG	3 cut(s)	533, 629, 866
BcoDI	GTCTC	2 cut(s)	310, 1040
BfaI	CTAG	2 cut(s)	1538, 1601
BfmI	CTRYAG	1 cut(s)	412
BfoI	RGCGCY	3 cut(s)	46, 174, 440
BfrI	CTTAAG	1 cut(s)	1072
BisI	GCNGC	5 cut(s)	41, 1061, 1098, 1303, 1506
BlpI	GCTNAGC	1 cut(s)	401
BlsI	GCNGC	5 cut(s)	42, 1062, 1099, 1304, 1507
Bme1390I	CCNGG	4 cut(s)	533, 629, 693, 866
Bme18I	GGWCC	2 cut(s)	250, 1340
BmeT110I	CYCGRG	2 cut(s)	317, 1088
BmgT120I	GGNCC	3 cut(s)	193, 250, 1340
BmiI	GGNNCC	2 cut(s)	395, 958
BmrFI	CCNGG	4 cut(s)	533, 629, 693, 866
BmrI	ACTGGG	2 cut(s)	296, 399
BmsI	GCATC	4 cut(s)	123, 781, 1260, 1444
BmuI	ACTGGG	2 cut(s)	296, 399
BoxI	GACNNNNGTC	1 cut(s)	1551
BpiI	GAAGAC	2 cut(s)	621, 1532
BpmI	CTGGAG	5 cut(s)	294, 816, 1452, 1518, 1570
Bpu10I	CCTNAGC	1 cut(s)	189
Bpu1102I	GCTNAGC	1 cut(s)	401
Bpu14I	TTCGAA	1 cut(s)	485
BpuMI	CCSGG	3 cut(s)	533, 629, 866
BsaAI	YACGTR	1 cut(s)	1172
BsaBI	GATNNNNATC	1 cut(s)	749
BsaHI	GRCGYC	2 cut(s)	870, 1063
BsaJI	CCNNGG	3 cut(s)	177, 531, 627
BsaWI	WCCGGW	2 cut(s)	1318, 1675
BsaXI	ACNNNNNCTCC	2 cut(s)	441, 471
Bsc4I	CCNNNNNNNGG	7 cut(s)	247, 317, 628, 821, 1019, 1202, 1296
Bse118I	RCCGGY	1 cut(s)	1675
Bse1I	ACTGG	4 cut(s)	302, 394, 1379, 1553
Bse21I	CCTNAGG	1 cut(s)	768
Bse3DI	GCAATG	1 cut(s)	934
Bse8I	GATNNNNATC	1 cut(s)	749
BseBI	CCWGG	1 cut(s)	693
BseDI	CCNNGG	3 cut(s)	177, 531, 627
BseGI	GGATG	4 cut(s)	719, 796, 919, 1427
BseJI	GATNNNNATC	1 cut(s)	749
BseLI	CCNNNNNNNGG	7 cut(s)	247, 317, 628, 821, 1019, 1202, 1296
BseMI	GCAATG	1 cut(s)	934
BseMII	CTCAG	6 cut(s)	203, 392, 782, 1148, 1674, 1702
BseNI	ACTGG	4 cut(s)	302, 394, 1379, 1553
BseRI	GAGGAG	4 cut(s)	179, 462, 947, 1455
BseSI	GKGCMC	2 cut(s)	327, 375
BseXI	GCAGC	2 cut(s)	1109, 1492
BseYI	CCCAGC	2 cut(s)	724, 1004
BsgI	GTGCAG	1 cut(s)	176
Bsh1236I	CGCG	1 cut(s)	1614
BshFI	GGCC	4 cut(s)	86, 194, 478, 1662
BshTI	ACCGGT	1 cut(s)	1675
BsiHKAI	GWGCWC	4 cut(s)	222, 327, 1611, 1689
BsiHKCI	CYCGRG	2 cut(s)	317, 1088
BsiSI	CCGG	7 cut(s)	74, 533, 629, 866, 1319, 1676, 1723
BslFI	GGGAC	2 cut(s)	532, 848
BslI	CCNNNNNNNGG	7 cut(s)	247, 317, 628, 821, 1019, 1202, 1296
BsmAI	GTCTC	2 cut(s)	310, 1040
BsmFI	GGGAC	2 cut(s)	532, 848
BsmI	GAATGC	4 cut(s)	266, 343, 1232, 1577
BsnI	GGCC	4 cut(s)	86, 194, 478, 1662
BsoBI	CYCGRG	2 cut(s)	317, 1088
Bsp119I	TTCGAA	1 cut(s)	485
Bsp1286I	GDGCHC	6 cut(s)	222, 327, 375, 1222, 1611, 1689
Bsp143I	GATC	8 cut(s)	78, 94, 127, 631, 811, 1045, 1256, 1360
Bsp1720I	GCTNAGC	1 cut(s)	401
BspACI	CCGC	9 cut(s)	40, 71, 123, 244, 764, 887, 1060, 1302, 1612
BspANI	GGCC	4 cut(s)	86, 194, 478, 1662
BspCNI	CTCAG	6 cut(s)	202, 393, 781, 1147, 1675, 1701
BspFNI	CGCG	1 cut(s)	1614
BspHI	TCATGA	1 cut(s)	117
BspLI	GGNNCC	2 cut(s)	395, 958
BspMAI	CTGCAG	1 cut(s)	416
BspPI	GGATC	4 cut(s)	639, 806, 1040, 1355
BspT104I	TTCGAA	1 cut(s)	485
BspTI	CTTAAG	1 cut(s)	1072
BsrDI	GCAATG	1 cut(s)	934
BsrFI	RCCGGY	1 cut(s)	1675
BsrI	ACTGG	4 cut(s)	302, 394, 1379, 1553
BssAI	RCCGGY	1 cut(s)	1675
BssECI	CCNNGG	3 cut(s)	177, 531, 627
BssMI	GATC	8 cut(s)	78, 94, 127, 631, 811, 1045, 1256, 1360
BssNI	GRCGYC	2 cut(s)	870, 1063
BssSI	CACGAG	1 cut(s)	1625
BssT1I	CCWWGG	1 cut(s)	177
Bst2BI	CACGAG	1 cut(s)	1625
Bst2UI	CCWGG	1 cut(s)	693
Bst4CI	ACNGT	2 cut(s)	684, 1591
Bst6I	CTCTTC	3 cut(s)	287, 812, 1328
BstACI	GRCGYC	2 cut(s)	870, 1063
BstAFI	CTTAAG	1 cut(s)	1072
BstAPI	GCANNNNNTGC	2 cut(s)	411, 791
BstBAI	YACGTR	1 cut(s)	1172
BstBI	TTCGAA	1 cut(s)	485
BstC8I	GCNNGC	1 cut(s)	54
BstDEI	CTNAG	8 cut(s)	189, 401, 651, 768, 1067, 1134, 1683, 1688
BstF5I	GGATG	4 cut(s)	719, 796, 919, 1427
BstFNI	CGCG	1 cut(s)	1614
BstH2I	RGCGCY	3 cut(s)	46, 174, 440
BstHHI	GCGC	4 cut(s)	45, 173, 343, 439
BstKTI	GATC	8 cut(s)	81, 97, 130, 634, 814, 1048, 1259, 1363
BstMAI	GTCTC	2 cut(s)	310, 1040
BstMBI	GATC	8 cut(s)	78, 94, 127, 631, 811, 1045, 1256, 1360
BstNI	CCWGG	1 cut(s)	693
BstNSI	RCATGY	3 cut(s)	575, 1639, 1671
BstPAI	GACNNNNGTC	1 cut(s)	1551
BstSCI	CCNGG	4 cut(s)	531, 627, 691, 864
BstSFI	CTRYAG	1 cut(s)	412
BstSLI	GKGCMC	2 cut(s)	327, 375
BstUI	CGCG	1 cut(s)	1614
BstV1I	GCAGC	2 cut(s)	1109, 1492
BstV2I	GAAGAC	2 cut(s)	621, 1532
BstX2I	RGATCY	2 cut(s)	631, 1045
BstYI	RGATCY	2 cut(s)	631, 1045
Bsu36I	CCTNAGG	1 cut(s)	768
BsuRI	GGCC	4 cut(s)	86, 194, 478, 1662
BtsCI	GGATG	4 cut(s)	719, 796, 919, 1427
BtsI	GCAGTG	2 cut(s)	412, 421
BtsIMutI	CAGTG	4 cut(s)	412, 421, 660, 1386
Cac8I	GCNNGC	1 cut(s)	54
CaiI	CAGNNNCTG	1 cut(s)	411
CciI	TCATGA	1 cut(s)	117
CfoI	GCGC	4 cut(s)	45, 173, 343, 439
Cfr10I	RCCGGY	1 cut(s)	1675
Cfr13I	GGNCC	3 cut(s)	193, 250, 1340
CseI	GACGC	3 cut(s)	717, 878, 1052
Csp6I	GTAC	6 cut(s)	277, 680, 1156, 1426, 1630, 1738
CspAI	ACCGGT	1 cut(s)	1675
CviAII	CATG	9 cut(s)	118, 329, 512, 572, 1001, 1214, 1636, 1668, 1759
CviQI	GTAC	6 cut(s)	277, 680, 1156, 1426, 1630, 1738
DdeI	CTNAG	8 cut(s)	189, 401, 651, 768, 1067, 1134, 1683, 1688
DpnI	GATC	8 cut(s)	80, 96, 129, 633, 813, 1047, 1258, 1362
DpnII	GATC	8 cut(s)	78, 94, 127, 631, 811, 1045, 1256, 1360
EaeI	YGGCCR	1 cut(s)	476
Eam1104I	CTCTTC	3 cut(s)	287, 812, 1328
EarI	CTCTTC	3 cut(s)	287, 812, 1328
Ecl136II	GAGCTC	2 cut(s)	220, 1687
Eco130I	CCWWGG	1 cut(s)	177
Eco147I	AGGCCT	1 cut(s)	86
Eco24I	GRGCYC	3 cut(s)	222, 1222, 1689
Eco47I	GGWCC	2 cut(s)	250, 1340
Eco47III	AGCGCT	1 cut(s)	438
Eco53kI	GAGCTC	2 cut(s)	220, 1687
Eco81I	CCTNAGG	1 cut(s)	768
Eco88I	CYCGRG	2 cut(s)	317, 1088
EcoICRI	GAGCTC	2 cut(s)	220, 1687
EcoO109I	RGGNCCY	2 cut(s)	193, 250
EcoRI	GAATTC	1 cut(s)	481
EcoRII	CCWGG	1 cut(s)	691
EcoT14I	CCWWGG	1 cut(s)	177
EcoT22I	ATGCAT	1 cut(s)	1459
EcoT38I	GRGCYC	3 cut(s)	222, 1222, 1689
ErhI	CCWWGG	1 cut(s)	177
FaeI	CATG	9 cut(s)	121, 332, 515, 575, 1004, 1217, 1639, 1671, 1762
FalI	AAGNNNNNCTT	2 cut(s)	266, 298
FaqI	GGGAC	2 cut(s)	532, 848
FatI	CATG	9 cut(s)	117, 328, 511, 571, 1000, 1213, 1635, 1667, 1758
Fnu4HI	GCNGC	5 cut(s)	41, 1061, 1098, 1303, 1506
FokI	GGATG	4 cut(s)	706, 803, 926, 1434
FriOI	GRGCYC	3 cut(s)	222, 1222, 1689
Fsp4HI	GCNGC	5 cut(s)	41, 1061, 1098, 1303, 1506
FspBI	CTAG	2 cut(s)	1538, 1601
GlaI	GCGC	4 cut(s)	44, 172, 342, 438
GluI	GCNGC	5 cut(s)	41, 1061, 1098, 1303, 1506
GsaI	CCCAGC	2 cut(s)	728, 1008
GsuI	CTGGAG	5 cut(s)	294, 816, 1452, 1518, 1570
HaeII	RGCGCY	3 cut(s)	46, 174, 440
HaeIII	GGCC	4 cut(s)	86, 194, 478, 1662
HapII	CCGG	7 cut(s)	74, 533, 629, 866, 1319, 1676, 1723
HgaI	GACGC	3 cut(s)	717, 878, 1052
HhaI	GCGC	4 cut(s)	45, 173, 343, 439
Hin1I	GRCGYC	2 cut(s)	870, 1063
Hin1II	CATG	9 cut(s)	121, 332, 515, 575, 1004, 1217, 1639, 1671, 1762
Hin6I	GCGC	4 cut(s)	43, 171, 341, 437
HinP1I	GCGC	4 cut(s)	43, 171, 341, 437
HincII	GTYRAC	1 cut(s)	1177
HindII	GTYRAC	1 cut(s)	1177
HindIII	AAGCTT	1 cut(s)	1751
HinfI	GANTC	4 cut(s)	141, 1178, 1552, 1699
HpaII	CCGG	7 cut(s)	74, 533, 629, 866, 1319, 1676, 1723
HphI	GGTGA	1 cut(s)	469
Hpy166II	GTNNAC	6 cut(s)	325, 1131, 1156, 1177, 1343, 1624
Hpy8I	GTNNAC	6 cut(s)	325, 1131, 1156, 1177, 1343, 1624
HpyAV	CCTTC	5 cut(s)	154, 1087, 1390, 1412, 1720
HpyCH4III	ACNGT	2 cut(s)	684, 1591
HpyCH4IV	ACGT	2 cut(s)	420, 1171
HpyF3I	CTNAG	8 cut(s)	189, 401, 651, 768, 1067, 1134, 1683, 1688
HpySE526I	ACGT	2 cut(s)	420, 1171
Hsp92I	GRCGYC	2 cut(s)	870, 1063
Hsp92II	CATG	9 cut(s)	121, 332, 515, 575, 1004, 1217, 1639, 1671, 1762
HspAI	GCGC	4 cut(s)	43, 171, 341, 437
Kzo9I	GATC	8 cut(s)	78, 94, 127, 631, 811, 1045, 1256, 1360
LmnI	GCTCC	1 cut(s)	1614
Lsp1109I	GCAGC	2 cut(s)	1109, 1492
LweI	GCATC	4 cut(s)	123, 781, 1260, 1444
MaeI	CTAG	2 cut(s)	1538, 1601
MaeII	ACGT	2 cut(s)	420, 1171
MaeIII	GTNAC	5 cut(s)	407, 416, 658, 729, 1102
MalI	GATC	8 cut(s)	80, 96, 129, 633, 813, 1047, 1258, 1362
MboI	GATC	8 cut(s)	78, 94, 127, 631, 811, 1045, 1256, 1360
MfeI	CAATTG	1 cut(s)	882
MflI	RGATCY	2 cut(s)	631, 1045
MhlI	GDGCHC	6 cut(s)	222, 327, 375, 1222, 1611, 1689
MlyI	GAGTC	2 cut(s)	1172, 1561
Mph1103I	ATGCAT	1 cut(s)	1459
MseI	TTAA	5 cut(s)	564, 989, 1073, 1586, 1715
MslI	CAYNNNNRTG	1 cut(s)	1452
MspA1I	CMGCKG	3 cut(s)	40, 400, 836
MspCI	CTTAAG	1 cut(s)	1072
MspI	CCGG	7 cut(s)	74, 533, 629, 866, 1319, 1676, 1723
MspR9I	CCNGG	4 cut(s)	533, 629, 693, 866
MunI	CAATTG	1 cut(s)	882
Mva1269I	GAATGC	4 cut(s)	266, 343, 1232, 1577
MvaI	CCWGG	1 cut(s)	693
MvnI	CGCG	1 cut(s)	1614
NciI	CCSGG	3 cut(s)	533, 629, 866
NdeII	GATC	8 cut(s)	78, 94, 127, 631, 811, 1045, 1256, 1360
NlaIII	CATG	9 cut(s)	121, 332, 515, 575, 1004, 1217, 1639, 1671, 1762
NlaIV	GGNNCC	2 cut(s)	395, 958
NmuCI	GTSAC	4 cut(s)	407, 416, 658, 729
NsiI	ATGCAT	1 cut(s)	1459
NspI	RCATGY	3 cut(s)	575, 1639, 1671
NspV	TTCGAA	1 cut(s)	485
PaeR7I	CTCGAG	1 cut(s)	1088
PagI	TCATGA	1 cut(s)	117
PceI	AGGCCT	1 cut(s)	86
PciI	ACATGT	1 cut(s)	1635
PctI	GAATGC	4 cut(s)	266, 343, 1232, 1577
PfeI	GAWTC	2 cut(s)	141, 1699
PflFI	GACNNNGTC	1 cut(s)	497
PflMI	CCANNNNNTGG	1 cut(s)	1202
PfoI	TCCNGGA	1 cut(s)	864
PinAI	ACCGGT	1 cut(s)	1675
PkrI	GCNGC	5 cut(s)	42, 1062, 1099, 1304, 1507
PleI	GAGTC	2 cut(s)	1172, 1560
PpsI	GAGTC	2 cut(s)	1172, 1560
Ppu21I	YACGTR	1 cut(s)	1172
PpuMI	RGGWCCY	1 cut(s)	250
PscI	ACATGT	1 cut(s)	1635
PshAI	GACNNNNGTC	1 cut(s)	1551
PsiI	TTATAA	1 cut(s)	1191
Psp124BI	GAGCTC	2 cut(s)	222, 1689
Psp5II	RGGWCCY	1 cut(s)	250
Psp6I	CCWGG	1 cut(s)	691
PspFI	CCCAGC	2 cut(s)	724, 1004
PspGI	CCWGG	1 cut(s)	691
PspN4I	GGNNCC	2 cut(s)	395, 958
PspPI	GGNCC	3 cut(s)	193, 250, 1340
PspPPI	RGGWCCY	1 cut(s)	250
PstI	CTGCAG	1 cut(s)	416
PstNI	CAGNNNCTG	1 cut(s)	411
PsuI	RGATCY	2 cut(s)	631, 1045
PsyI	GACNNNGTC	1 cut(s)	497
PvuII	CAGCTG	2 cut(s)	400, 836
RsaI	GTAC	6 cut(s)	278, 681, 1157, 1427, 1631, 1739
RsaNI	GTAC	6 cut(s)	277, 680, 1156, 1426, 1630, 1738
RseI	CAYNNNNRTG	1 cut(s)	1452
SacI	GAGCTC	2 cut(s)	222, 1689
SaqAI	TTAA	5 cut(s)	564, 989, 1073, 1586, 1715
SatI	GCNGC	5 cut(s)	41, 1061, 1098, 1303, 1506
Sau3AI	GATC	8 cut(s)	78, 94, 127, 631, 811, 1045, 1256, 1360
Sau96I	GGNCC	3 cut(s)	193, 250, 1340
SchI	GAGTC	2 cut(s)	1172, 1561
ScrFI	CCNGG	4 cut(s)	533, 629, 693, 866
SduI	GDGCHC	6 cut(s)	222, 327, 375, 1222, 1611, 1689
SfaNI	GCATC	4 cut(s)	123, 781, 1260, 1444
SfcI	CTRYAG	1 cut(s)	412
Sfr274I	CTCGAG	1 cut(s)	1088
SfuI	TTCGAA	1 cut(s)	485
SinI	GGWCC	2 cut(s)	250, 1340
SlaI	CTCGAG	1 cut(s)	1088
SmiMI	CAYNNNNRTG	1 cut(s)	1452
SmlI	CTYRAG	2 cut(s)	1072, 1088
SmoI	CTYRAG	2 cut(s)	1072, 1088
SseBI	AGGCCT	1 cut(s)	86
SsiI	CCGC	9 cut(s)	40, 71, 123, 244, 764, 887, 1060, 1302, 1612
SspI	AATATT	1 cut(s)	1144
SspMI	CTAG	2 cut(s)	1538, 1601
SstI	GAGCTC	2 cut(s)	222, 1689
StuI	AGGCCT	1 cut(s)	86
StyD4I	CCNGG	4 cut(s)	531, 627, 691, 864
StyI	CCWWGG	1 cut(s)	177
TaaI	ACNGT	2 cut(s)	684, 1591
TaiI	ACGT	2 cut(s)	423, 1174
TauI	GCSGC	3 cut(s)	43, 1063, 1305
TfiI	GAWTC	2 cut(s)	141, 1699
Tru1I	TTAA	5 cut(s)	564, 989, 1073, 1586, 1715
Tru9I	TTAA	5 cut(s)	564, 989, 1073, 1586, 1715
TscAI	CASTG	4 cut(s)	412, 421, 667, 1386
TseFI	GTSAC	4 cut(s)	407, 416, 658, 729
TseI	GCWGC	2 cut(s)	1097, 1505
Tsp45I	GTSAC	4 cut(s)	407, 416, 658, 729
TspDTI	ATGAA	7 cut(s)	349, 350, 500, 921, 930, 989, 1535
TspGWI	ACGGA	3 cut(s)	164, 955, 1735
TspRI	CASTG	4 cut(s)	412, 421, 667, 1386
Tth111I	GACNNNGTC	1 cut(s)	497
Van91I	CCANNNNNTGG	1 cut(s)	1202
Vha464I	CTTAAG	1 cut(s)	1072
VneI	GTGCAC	1 cut(s)	323
VpaK11BI	GGWCC	2 cut(s)	250, 1340
XapI	RAATTY	3 cut(s)	481, 713, 1571
XceI	RCATGY	3 cut(s)	575, 1639, 1671
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1203
XhoI	CTCGAG	1 cut(s)	1088
XspI	CTAG	2 cut(s)	1538, 1601
Zsp2I	ATGCAT	1 cut(s)	1459

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.