Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0009798

Physical Location & Seq

Genomic Coordinates Forward (+)

29889957 .. 29894292

Gene Structure 4336 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1221900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1785 bp

ATGTCCAAACAGACCTACAGAGTCTGCTTCTGCTTCCAGCGGCGCTTCAAGCTCGCCGTCTCGGAAGCACCGCCGGAGGTCAGGGCCTTGTTCGATCAGTACTCGGAGAACGGCATCATGACCGCCGATCACTTCCACCGCTTTCTGAAGGAAGTCCAGAAGGAGGAGAGCGCCACCAAGGAGTTAGCTCAGGCCCTCATCGAACAGAGCCTCGCCGAGTTCAAACACCTCAACATCTTCCACCGCAAGGTCTTCAATCTCGAAGCATTCTTCAAATACCTCTTCGGCGACATCAACCCCCCAATCTCTCCAGCTCTCGGGGTGCACCATGATATGAGTGCGCCATTGTCGCATTATTTCATACACACAGGGCACAACTCTTATCTGACCGGGAACCAGCTGAGCAGTCACTGCAGTGACGTTCCGATTATAGAAGCTCTTCGCAGAGGAGTGAAGGTGATTGAGCTGGATATTTGGCCTAATTCGAATAAAGATGATGTCGATGTGCTTCACGGAGGGACATTGACTACACCAGTGGAACTTATCAAGTGTTTGAGGTCTATTAAGGAACATGCCTTTGCTGCCTCTGAATACCCTGTTGTGATTACTTTAGAAGACCACCTTACCCCTGATCTTCAAGCCAAAGTCGCTCAGATGCTCACTGAAACATTTGGGGGCGTACTGTTTCATCCTGGCTCGGAGTGTTTGAAAGAATTTCCATCCCCAGCGTCACTAAAGAAAAGGATTATTATTTCAACCAAACCGCCCAAGAAATATCCTGAGGCAAAGAATGCTGTGCAAGGAGAAAGTGATCCAAAGAGTGGAAAGACTCCAGCTGATGAGGAAGCGTGGGGTAAAGAAGTTCCGGACGCCAAAGGTGCAACTGTGGCAAATGACACGGATGAATTGGATAAAGACGATAGCACTGAGGAGGAAGATATTGATTATGGTGGTTCCAAGTCCGATCAAAATGTAGCACCTGAATATAAGCATTTAATAGCCATTCATGCTGGGAAACCTAAAGGTGGATTAGCAGAGTGTCTCAAAGTAGATACAGATAAAGTGCGGCGTATAAGCTTAAGTGAGCAACAGCTCGAGAAGGCAGTAGTAACGTACGGAAAGGAAATTGTCAGGTTTACTCAGAGGAATATTCTGAGGGTGTACCCAAAGGGCATACGTGTGGACTCATCAAATTACAACCCAATGATTGGGTGGTCGTATGGGGCTCAAATGGTTGCATTTAATATGCAGGGATATGGAAGACCATTGTGGGTGATGCAGGGAATGTTTAGAGCCAATGGTAGGTGCGGCTATGTGAAAAAACCGGATTTTCTTTTGAAGAGTGGTCCACATAATGAGGTGTTTGATCCTAAATATAGTTTACCAGTGAAAAAAACTTTGAAGGTAAAAGTATATATGGGAGAAGGATGGTACTATGATTTCAAGCACACACACTTTGATGCGTATTCTCCTCCAGATTTTTATACAAGGGTAGGAATTGCTGGAGTTGCTGATGATAGCGTGATGAAAAAGACGAAGGCCCTAGAGGACAATTGGATTCCGGCCTGGAACGAAGAATTCGAATTCCCATTAACCGTTCCAGAACTAGCTGTGCTCCGCATTGAAGTTCATGAGTACGACATGTCAGAGAAGGACGACTTTGGAGGCCAAACCTGCCTGCCGGTGTCTGAGCTACGTAGAGGGATTCGAGCAGTTCCCTTACACTGCCCTAAGGGAGAGAAGTACAAATCGGTGAAGCTTCTTATGCACTTCGATTTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004629 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007049 GO:0007275 GO:0008081 GO:0008150 GO:0009553 GO:0009555 GO:0009556 GO:0009605 GO:0009607 GO:0009617 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010600 GO:0010601 GO:0010817 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0034293 GO:0042578 GO:0042742 GO:0043207 GO:0043934 GO:0044464 GO:0044703 GO:0046885 GO:0046886 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051707 GO:0061458 GO:0065007 GO:0065008 GO:0071944 GO:0090354 GO:0090355 GO:0090567 GO:0098542 GO:0099402 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933

Pfam Domains

Protein Families

C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

595

Amino Acids

67.18

Weight (kDa)

6.36

Isoelectric Point (pI)

44.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_like	PF09279	26 - 69	5.4e-06	Phosphoinositide-specific phospholipase C, efhand-like
PI-PLC-X	PF00388	112 - 254	2.2e-51	Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y	PF00387	357 - 444	2.2e-29	Phosphatidylinositol-specific phospholipase C, Y domain
C2	PF00168	466 - 567	9.8e-13	C2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009798)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08510 AT3G08510 AT3G08510 AT3G08510 AT3G55940
fragaria_vesca	FvH4_7g24471 FvH4_7g24471
malus_domestica	MD01G1153000.v1.1 MD07G1221900.v1.1
prunus_persica	Prupe.2G250100_v2.0.a1
pyrus_communis	pycom01g17060 pycom07g20040
rosa_chinensis	RchiOBHm_Chr1g0370231
rosa_laevigata	RLG00000027067
rosa_multiflora	Rmu_sc0017405.1_g000001 Rmu_sc0030456.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286730
rosa_rugosa	Rorug01G0354700 Rorug01G0354800
rosa_samantha	Rh1AG363500
rosa_wichuraiana	Rw1G031990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1682
AccB7I	CCANNNNNTGG	2 cut(s)	821, 1208
AccIII	TCCGGA	1 cut(s)	865
AciI	CCGC	9 cut(s)	40, 71, 123, 139, 244, 764, 1066, 1308, 1618
AclWI	GGATC	2 cut(s)	806, 1361
AcsI	RAATTY	3 cut(s)	713, 1577, 1583
AcuI	CTGAAG	1 cut(s)	167
AcyI	GRCGYC	1 cut(s)	870
AfaI	GTAC	7 cut(s)	101, 681, 1115, 1163, 1433, 1637, 1745
AfiI	CCNNNNNNNGG	7 cut(s)	163, 247, 317, 821, 1025, 1208, 1302
AflII	CTTAAG	1 cut(s)	1078
AflIII	ACRYGT	2 cut(s)	1177, 1641
AjnI	CCWGG	2 cut(s)	691, 1565
AleI	CACNNNNGTG	1 cut(s)	414
Alw21I	GWGCWC	2 cut(s)	327, 1617
Alw26I	GTCTC	2 cut(s)	64, 1046
Alw44I	GTGCAC	1 cut(s)	323
AlwI	GGATC	2 cut(s)	806, 1361
AlwNI	CAGNNNCTG	2 cut(s)	24, 411
Ama87I	CYCGRG	2 cut(s)	317, 1094
Aor13HI	TCCGGA	1 cut(s)	865
AoxI	GGCC	6 cut(s)	84, 192, 476, 1539, 1563, 1666
ApaLI	GTGCAC	1 cut(s)	323
ApeKI	GCWGC	1 cut(s)	581
ApoI	RAATTY	3 cut(s)	713, 1577, 1583
Asp700I	GAANNNNTTC	1 cut(s)	438
AspLEI	GCGC	3 cut(s)	45, 173, 343
AspS9I	GGNCC	4 cut(s)	84, 193, 1346, 1540
AsuC2I	CCSGG	1 cut(s)	391
AsuHPI	GGTGA	3 cut(s)	469, 1285, 1765
AsuII	TTCGAA	2 cut(s)	485, 1581
AvaI	CYCGRG	2 cut(s)	317, 1094
AvaII	GGWCC	1 cut(s)	1346
AxyI	CCTNAGG	2 cut(s)	780, 1731
BaeGI	GKGCMC	2 cut(s)	327, 375
BanII	GRGCYC	1 cut(s)	1228
BbsI	GAAGAC	3 cut(s)	244, 621, 1267
Bbv12I	GWGCWC	2 cut(s)	327, 1617
BbvI	GCAGC	1 cut(s)	568
BccI	CCATC	2 cut(s)	727, 1422
BceAI	ACGGC	2 cut(s)	41, 127
BciT130I	CCWGG	2 cut(s)	693, 1567
BcnI	CCSGG	1 cut(s)	391
BcoDI	GTCTC	2 cut(s)	64, 1046
BfaI	CTAG	2 cut(s)	1544, 1607
BfmI	CTRYAG	2 cut(s)	16, 412
BfoI	RGCGCY	2 cut(s)	46, 174
BfrI	CTTAAG	1 cut(s)	1078
BfuAI	ACCTGC	1 cut(s)	1682
BisI	GCNGC	4 cut(s)	41, 582, 1067, 1309
BlpI	GCTNAGC	1 cut(s)	401
BlsI	GCNGC	4 cut(s)	42, 583, 1068, 1310
BmcAI	AGTACT	1 cut(s)	101
Bme1390I	CCNGG	3 cut(s)	391, 693, 1567
Bme18I	GGWCC	1 cut(s)	1346
BmeT110I	CYCGRG	2 cut(s)	317, 1094
BmgT120I	GGNCC	4 cut(s)	84, 193, 1346, 1540
BmiI	GGNNCC	2 cut(s)	395, 955
BmrFI	CCNGG	3 cut(s)	391, 693, 1567
BmsI	GCATC	4 cut(s)	123, 645, 1266, 1450
BpiI	GAAGAC	3 cut(s)	244, 621, 1267
BpmI	CTGGAG	4 cut(s)	294, 816, 1458, 1524
Bpu10I	CCTNAGC	1 cut(s)	189
Bpu1102I	GCTNAGC	1 cut(s)	401
Bpu14I	TTCGAA	2 cut(s)	485, 1581
BpuMI	CCSGG	1 cut(s)	391
BsaAI	YACGTR	2 cut(s)	1178, 1697
BsaHI	GRCGYC	1 cut(s)	870
BsaJI	CCNNGG	1 cut(s)	177
BsaWI	WCCGGW	2 cut(s)	865, 1324
BsaXI	ACNNNNNCTCC	2 cut(s)	441, 471
Bsc4I	CCNNNNNNNGG	7 cut(s)	163, 247, 317, 821, 1025, 1208, 1302
Bse118I	RCCGGY	1 cut(s)	1681
Bse1I	ACTGG	2 cut(s)	533, 1385
Bse21I	CCTNAGG	2 cut(s)	780, 1731
BseAI	TCCGGA	1 cut(s)	865
BseBI	CCWGG	2 cut(s)	693, 1567
BseDI	CCNNGG	1 cut(s)	177
BseGI	GGATG	4 cut(s)	688, 719, 907, 1433
BseLI	CCNNNNNNNGG	7 cut(s)	163, 247, 317, 821, 1025, 1208, 1302
BseMII	CTCAG	8 cut(s)	203, 392, 665, 771, 918, 1145, 1154, 1680
BseNI	ACTGG	2 cut(s)	533, 1385
BseRI	GAGGAG	4 cut(s)	179, 462, 944, 1461
BseSI	GKGCMC	2 cut(s)	327, 375
BseXI	GCAGC	1 cut(s)	568
BseYI	CCCAGC	2 cut(s)	724, 1010
BshFI	GGCC	6 cut(s)	86, 194, 478, 1541, 1565, 1668
BsiHKAI	GWGCWC	2 cut(s)	327, 1617
BsiHKCI	CYCGRG	2 cut(s)	317, 1094
BsiSI	CCGG	6 cut(s)	74, 390, 866, 1325, 1562, 1682
BsiWI	CGTACG	1 cut(s)	1113
BslFI	GGGAC	1 cut(s)	532
BslI	CCNNNNNNNGG	7 cut(s)	163, 247, 317, 821, 1025, 1208, 1302
BsmAI	GTCTC	2 cut(s)	64, 1046
BsmBI	CGTCTC	1 cut(s)	64
BsmFI	GGGAC	1 cut(s)	532
BsmI	GAATGC	2 cut(s)	266, 796
BsnI	GGCC	6 cut(s)	86, 194, 478, 1541, 1565, 1668
BsoBI	CYCGRG	2 cut(s)	317, 1094
Bsp119I	TTCGAA	2 cut(s)	485, 1581
Bsp1286I	GDGCHC	4 cut(s)	327, 375, 1228, 1617
Bsp13I	TCCGGA	1 cut(s)	865
Bsp143I	GATC	6 cut(s)	94, 127, 631, 811, 964, 1366
Bsp1720I	GCTNAGC	1 cut(s)	401
BspACI	CCGC	9 cut(s)	40, 71, 123, 139, 244, 764, 1066, 1308, 1618
BspANI	GGCC	6 cut(s)	86, 194, 478, 1541, 1565, 1668
BspCNI	CTCAG	8 cut(s)	202, 393, 664, 772, 919, 1146, 1153, 1681
BspEI	TCCGGA	1 cut(s)	865
BspHI	TCATGA	2 cut(s)	117, 1630
BspLI	GGNNCC	2 cut(s)	395, 955
BspMAI	CTGCAG	1 cut(s)	416
BspMI	ACCTGC	1 cut(s)	1682
BspPI	GGATC	2 cut(s)	806, 1361
BspQI	GCTCTTC	1 cut(s)	444
BspT104I	TTCGAA	2 cut(s)	485, 1581
BspTI	CTTAAG	1 cut(s)	1078
BsrFI	RCCGGY	1 cut(s)	1681
BsrI	ACTGG	2 cut(s)	533, 1385
BssAI	RCCGGY	1 cut(s)	1681
BssECI	CCNNGG	1 cut(s)	177
BssMI	GATC	6 cut(s)	94, 127, 631, 811, 964, 1366
BssNI	GRCGYC	1 cut(s)	870
BssT1I	CCWWGG	1 cut(s)	177
Bst2UI	CCWGG	2 cut(s)	693, 1567
Bst4CI	ACNGT	3 cut(s)	684, 886, 1597
Bst6I	CTCTTC	3 cut(s)	287, 444, 1334
BstACI	GRCGYC	1 cut(s)	870
BstAFI	CTTAAG	1 cut(s)	1078
BstAPI	GCANNNNNTGC	2 cut(s)	411, 791
BstBAI	YACGTR	2 cut(s)	1178, 1697
BstBI	TTCGAA	2 cut(s)	485, 1581
BstC8I	GCNNGC	2 cut(s)	54, 1679
BstDEI	CTNAG	9 cut(s)	189, 401, 651, 780, 927, 1140, 1154, 1689, 1731
BstF5I	GGATG	4 cut(s)	688, 719, 907, 1433
BstH2I	RGCGCY	2 cut(s)	46, 174
BstHHI	GCGC	3 cut(s)	45, 173, 343
BstKTI	GATC	6 cut(s)	97, 130, 634, 814, 967, 1369
BstMAI	GTCTC	2 cut(s)	64, 1046
BstMBI	GATC	6 cut(s)	94, 127, 631, 811, 964, 1366
BstNI	CCWGG	2 cut(s)	693, 1567
BstNSI	RCATGY	2 cut(s)	575, 1645
BstSCI	CCNGG	3 cut(s)	389, 691, 1565
BstSFI	CTRYAG	2 cut(s)	16, 412
BstSLI	GKGCMC	2 cut(s)	327, 375
BstSNI	TACGTA	1 cut(s)	1697
BstV1I	GCAGC	1 cut(s)	568
BstV2I	GAAGAC	3 cut(s)	244, 621, 1267
Bsu36I	CCTNAGG	2 cut(s)	780, 1731
BsuRI	GGCC	6 cut(s)	86, 194, 478, 1541, 1565, 1668
BtsCI	GGATG	4 cut(s)	688, 719, 907, 1433
BtsI	GCAGTG	3 cut(s)	409, 421, 1723
BtsIMutI	CAGTG	7 cut(s)	409, 421, 540, 660, 924, 1392, 1723
BveI	ACCTGC	1 cut(s)	1682
Cac8I	GCNNGC	2 cut(s)	54, 1679
CaiI	CAGNNNCTG	2 cut(s)	24, 411
CciI	TCATGA	2 cut(s)	117, 1630
CfoI	GCGC	3 cut(s)	45, 173, 343
Cfr10I	RCCGGY	1 cut(s)	1681
Cfr13I	GGNCC	4 cut(s)	84, 193, 1346, 1540
CseI	GACGC	2 cut(s)	717, 878
Csp6I	GTAC	7 cut(s)	100, 680, 1114, 1162, 1432, 1636, 1744
CviAII	CATG	6 cut(s)	118, 329, 572, 1007, 1631, 1642
CviQI	GTAC	7 cut(s)	100, 680, 1114, 1162, 1432, 1636, 1744
DdeI	CTNAG	9 cut(s)	189, 401, 651, 780, 927, 1140, 1154, 1689, 1731
DpnI	GATC	6 cut(s)	96, 129, 633, 813, 966, 1368
DpnII	GATC	6 cut(s)	94, 127, 631, 811, 964, 1366
Eam1104I	CTCTTC	3 cut(s)	287, 444, 1334
EarI	CTCTTC	3 cut(s)	287, 444, 1334
Eco105I	TACGTA	1 cut(s)	1697
Eco130I	CCWWGG	1 cut(s)	177
Eco24I	GRGCYC	1 cut(s)	1228
Eco47I	GGWCC	1 cut(s)	1346
Eco57I	CTGAAG	1 cut(s)	167
Eco81I	CCTNAGG	2 cut(s)	780, 1731
Eco88I	CYCGRG	2 cut(s)	317, 1094
EcoO109I	RGGNCCY	3 cut(s)	84, 193, 1540
EcoRI	GAATTC	2 cut(s)	1577, 1583
EcoRII	CCWGG	2 cut(s)	691, 1565
EcoT14I	CCWWGG	1 cut(s)	177
EcoT38I	GRGCYC	1 cut(s)	1228
ErhI	CCWWGG	1 cut(s)	177
Esp3I	CGTCTC	1 cut(s)	64
FaeI	CATG	6 cut(s)	121, 332, 575, 1010, 1634, 1645
FalI	AAGNNNNNCTT	4 cut(s)	606, 638, 1643, 1675
FaqI	GGGAC	1 cut(s)	532
FatI	CATG	6 cut(s)	117, 328, 571, 1006, 1630, 1641
Fnu4HI	GCNGC	4 cut(s)	41, 582, 1067, 1309
FokI	GGATG	4 cut(s)	675, 706, 914, 1440
FriOI	GRGCYC	1 cut(s)	1228
Fsp4HI	GCNGC	4 cut(s)	41, 582, 1067, 1309
FspBI	CTAG	2 cut(s)	1544, 1607
GlaI	GCGC	3 cut(s)	44, 172, 342
GluI	GCNGC	4 cut(s)	41, 582, 1067, 1309
GsaI	CCCAGC	2 cut(s)	728, 1014
GsuI	CTGGAG	4 cut(s)	294, 816, 1458, 1524
HaeII	RGCGCY	2 cut(s)	46, 174
HaeIII	GGCC	6 cut(s)	86, 194, 478, 1541, 1565, 1668
HapII	CCGG	6 cut(s)	74, 390, 866, 1325, 1562, 1682
HgaI	GACGC	2 cut(s)	717, 878
HhaI	GCGC	3 cut(s)	45, 173, 343
Hin1I	GRCGYC	1 cut(s)	870
Hin1II	CATG	6 cut(s)	121, 332, 575, 1010, 1634, 1645
Hin6I	GCGC	3 cut(s)	43, 171, 341
HinP1I	GCGC	3 cut(s)	43, 171, 341
HindIII	AAGCTT	2 cut(s)	1075, 1757
HinfI	GANTC	5 cut(s)	21, 829, 1184, 1558, 1705
HpaII	CCGG	6 cut(s)	74, 390, 866, 1325, 1562, 1682
HphI	GGTGA	3 cut(s)	469, 1285, 1765
Hpy166II	GTNNAC	6 cut(s)	325, 1137, 1162, 1183, 1349, 1382
Hpy188III	TCNNGA	9 cut(s)	118, 157, 260, 779, 866, 1096, 1475, 1601, 1631
Hpy8I	GTNNAC	6 cut(s)	325, 1137, 1162, 1183, 1349, 1382
HpyAV	CCTTC	8 cut(s)	142, 154, 448, 1093, 1396, 1418, 1531, 1645
HpyCH4III	ACNGT	3 cut(s)	684, 886, 1597
HpyCH4IV	ACGT	4 cut(s)	420, 1112, 1177, 1696
HpyCH4V	TGCA	8 cut(s)	325, 414, 799, 881, 1238, 1249, 1279, 1768
HpyF3I	CTNAG	9 cut(s)	189, 401, 651, 780, 927, 1140, 1154, 1689, 1731
HpySE526I	ACGT	4 cut(s)	420, 1112, 1177, 1696
Hsp92I	GRCGYC	1 cut(s)	870
Hsp92II	CATG	6 cut(s)	121, 332, 575, 1010, 1634, 1645
HspAI	GCGC	3 cut(s)	43, 171, 341
Kpn2I	TCCGGA	1 cut(s)	865
Kzo9I	GATC	6 cut(s)	94, 127, 631, 811, 964, 1366
LguI	GCTCTTC	1 cut(s)	444
LmnI	GCTCC	1 cut(s)	1620
Lsp1109I	GCAGC	1 cut(s)	568
LweI	GCATC	4 cut(s)	123, 645, 1266, 1450
MaeI	CTAG	2 cut(s)	1544, 1607
MaeII	ACGT	4 cut(s)	420, 1112, 1177, 1696
MaeIII	GTNAC	4 cut(s)	407, 416, 729, 1108
MalI	GATC	6 cut(s)	96, 129, 633, 813, 966, 1368
MboI	GATC	6 cut(s)	94, 127, 631, 811, 964, 1366
MfeI	CAATTG	1 cut(s)	1552
MhlI	GDGCHC	4 cut(s)	327, 375, 1228, 1617
MluCI	AATT	9 cut(s)	481, 713, 905, 1125, 1192, 1497, 1552, 1577, 1583
MlyI	GAGTC	3 cut(s)	30, 823, 1178
MroI	TCCGGA	1 cut(s)	865
MroXI	GAANNNNTTC	1 cut(s)	438
MseI	TTAA	5 cut(s)	564, 995, 1079, 1242, 1592
MslI	CAYNNNNRTG	3 cut(s)	414, 1178, 1458
MspA1I	CMGCKG	3 cut(s)	40, 400, 836
MspCI	CTTAAG	1 cut(s)	1078
MspI	CCGG	6 cut(s)	74, 390, 866, 1325, 1562, 1682
MspR9I	CCNGG	3 cut(s)	391, 693, 1567
MunI	CAATTG	1 cut(s)	1552
Mva1269I	GAATGC	2 cut(s)	266, 796
MvaI	CCWGG	2 cut(s)	693, 1567
NciI	CCSGG	1 cut(s)	391
NdeII	GATC	6 cut(s)	94, 127, 631, 811, 964, 1366
NlaIII	CATG	6 cut(s)	121, 332, 575, 1010, 1634, 1645
NlaIV	GGNNCC	2 cut(s)	395, 955
NmeAIII	GCCGAG	1 cut(s)	241
NmuCI	GTSAC	3 cut(s)	407, 416, 729
NspI	RCATGY	2 cut(s)	575, 1645
NspV	TTCGAA	2 cut(s)	485, 1581
OliI	CACNNNNGTG	1 cut(s)	414
PaeR7I	CTCGAG	1 cut(s)	1094
PagI	TCATGA	2 cut(s)	117, 1630
PciI	ACATGT	1 cut(s)	1641
PciSI	GCTCTTC	1 cut(s)	444
PcsI	WCGNNNNNNNCGW	1 cut(s)	1578
PctI	GAATGC	2 cut(s)	266, 796
PdmI	GAANNNNTTC	1 cut(s)	438
PfeI	GAWTC	2 cut(s)	1558, 1705
Pfl23II	CGTACG	1 cut(s)	1113
PflMI	CCANNNNNTGG	2 cut(s)	821, 1208
PkrI	GCNGC	4 cut(s)	42, 583, 1068, 1310
PleI	GAGTC	3 cut(s)	29, 823, 1178
PpsI	GAGTC	3 cut(s)	29, 823, 1178
Ppu21I	YACGTR	2 cut(s)	1178, 1697
PscI	ACATGT	1 cut(s)	1641
Psp6I	CCWGG	2 cut(s)	691, 1565
PspFI	CCCAGC	2 cut(s)	724, 1010
PspGI	CCWGG	2 cut(s)	691, 1565
PspLI	CGTACG	1 cut(s)	1113
PspN4I	GGNNCC	2 cut(s)	395, 955
PspPI	GGNCC	4 cut(s)	84, 193, 1346, 1540
PstI	CTGCAG	1 cut(s)	416
PstNI	CAGNNNCTG	2 cut(s)	24, 411
PvuII	CAGCTG	2 cut(s)	400, 836
RsaI	GTAC	7 cut(s)	101, 681, 1115, 1163, 1433, 1637, 1745
RsaNI	GTAC	7 cut(s)	100, 680, 1114, 1162, 1432, 1636, 1744
RseI	CAYNNNNRTG	3 cut(s)	414, 1178, 1458
SapI	GCTCTTC	1 cut(s)	444
SaqAI	TTAA	5 cut(s)	564, 995, 1079, 1242, 1592
SatI	GCNGC	4 cut(s)	41, 582, 1067, 1309
Sau3AI	GATC	6 cut(s)	94, 127, 631, 811, 964, 1366
Sau96I	GGNCC	4 cut(s)	84, 193, 1346, 1540
ScaI	AGTACT	1 cut(s)	101
SchI	GAGTC	3 cut(s)	30, 823, 1178
ScrFI	CCNGG	3 cut(s)	391, 693, 1567
SduI	GDGCHC	4 cut(s)	327, 375, 1228, 1617
SfaNI	GCATC	4 cut(s)	123, 645, 1266, 1450
SfcI	CTRYAG	2 cut(s)	16, 412
Sfr274I	CTCGAG	1 cut(s)	1094
SfuI	TTCGAA	2 cut(s)	485, 1581
SinI	GGWCC	1 cut(s)	1346
SlaI	CTCGAG	1 cut(s)	1094
SmiMI	CAYNNNNRTG	3 cut(s)	414, 1178, 1458
SmlI	CTYRAG	2 cut(s)	1078, 1094
SmoI	CTYRAG	2 cut(s)	1078, 1094
SnaBI	TACGTA	1 cut(s)	1697
Sse9I	AATT	9 cut(s)	481, 713, 905, 1125, 1192, 1497, 1552, 1577, 1583
SsiI	CCGC	9 cut(s)	40, 71, 123, 139, 244, 764, 1066, 1308, 1618
SspI	AATATT	1 cut(s)	1150
SspMI	CTAG	2 cut(s)	1544, 1607
StyD4I	CCNGG	3 cut(s)	389, 691, 1565
StyI	CCWWGG	1 cut(s)	177
TaaI	ACNGT	3 cut(s)	684, 886, 1597
TaiI	ACGT	4 cut(s)	423, 1115, 1180, 1699
TaqI	TCGA	9 cut(s)	93, 201, 261, 485, 501, 1095, 1581, 1708, 1773
TasI	AATT	9 cut(s)	481, 713, 905, 1125, 1192, 1497, 1552, 1577, 1583
TatI	WGTACW	2 cut(s)	99, 1743
TauI	GCSGC	3 cut(s)	43, 1069, 1311
TfiI	GAWTC	2 cut(s)	1558, 1705
Tru1I	TTAA	5 cut(s)	564, 995, 1079, 1242, 1592
Tru9I	TTAA	5 cut(s)	564, 995, 1079, 1242, 1592
TscAI	CASTG	7 cut(s)	416, 421, 540, 667, 931, 1392, 1730
TseFI	GTSAC	3 cut(s)	407, 416, 729
TseI	GCWGC	1 cut(s)	581
Tsp45I	GTSAC	3 cut(s)	407, 416, 729
TspDTI	ATGAA	6 cut(s)	349, 677, 918, 995, 1541, 1619
TspGWI	ACGGA	3 cut(s)	528, 914, 1131
TspRI	CASTG	7 cut(s)	416, 421, 540, 667, 931, 1392, 1730
Van91I	CCANNNNNTGG	2 cut(s)	821, 1208
Vha464I	CTTAAG	1 cut(s)	1078
VneI	GTGCAC	1 cut(s)	323
VpaK11BI	GGWCC	1 cut(s)	1346
XapI	RAATTY	3 cut(s)	713, 1577, 1583
XceI	RCATGY	2 cut(s)	575, 1645
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1209
XhoI	CTCGAG	1 cut(s)	1094
XmnI	GAANNNNTTC	1 cut(s)	438
XspI	CTAG	2 cut(s)	1544, 1607
ZrmI	AGTACT	1 cut(s)	101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.