MD01G1184600.v1.1

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014954
Physical Location & Seq
Reverse (-)
28384993 .. 28386399
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1184600.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGCCCCAGCACCGCCGTACCGGCTGGCTTTCTCAGCCACCGGCTCCGGTGCTCCTTCTCCTCACGGTACTCACCCTCTTCCTCTCCGCCAACCCAGCCATTGCATGGCGCCCCTGGCCCCACCTCAAACACAACACCTCCGACCTCCGCTTCGGAGACAACAAAAAATTCGAAGGCTCCTCCGATTTCGTCAAGTTGCGCTACCACATGGGCCCTGTACTAACCGCCAACATAACCGTCCACACAATATGGTACGGCACGTGGCAGCCGCGCCAGAAGAAGATCATCCGCGAGTTCATCAACTCCATCTCTGCCGCCGGCTCCAAGCGCCCCTCCGTCGCCGGTTGGTGGAGGACCGTACAGCTTTACACCGACCAGACCGGCGCGAACATCTCGCGCACGGTCCAGCTCGGCGCCGGGAAAAACGACCGGTTTTACTCTCACGGTAAAACCCTGACGCGCCTCTCGATACAGTCCGTGATCAAAAGCGCCGTAACGGCTAAAACGAGGCCGTTACCCGTCAATCCACAGAGTGGGCTCTACCTCCTGCTCACATCTGATGACGTGTACGTCCAGGATTTCTGCAAACAGGTCTGTGGATTCCACTACTTTACTTTCCCCTCCCTCGTCGGATACACGCTGCCGTACGCGTGGATTGGAAACTCGGCCAAATTGTGTCCAGGTACGTGCGCCTACCCGTTCGCCGTACCCGATTATATCCCGGGGTTAAAGCCGCTAAAGTCACCTAACGGTGACGTGGGAGTTGAAGGGATGATAAGCGTTATCGCGCACGAGATTGCTGAGCTGGCGAGTAACCCGTTAGTGAACGCCTGGTACGCGGGTCAGGACCCGAGTTTTCCGGTCGAGATTGCTGATCTCTGCGAGGGAATCTACGGTACGGGAGGGGGCGGGTCGTACACGGGTCAGCTGATGGACGATCACGATGGTGCCACGTATAATATGAATGGGATCCGACGGAAGTTCTTGGTTCAGTGGGTTTGGAGCCACATTTTAAATTACTGTACCGGACCTAATGCACTCGATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0008150 GO:0009505 GO:0009506 GO:0009628 GO:0030054 GO:0030312 GO:0044464 GO:0050896 GO:0055044 GO:0071944 GO:0080167
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

350

Amino Acids

38.71

Weight (kDa)

9.11

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 66 - 343 2e-100 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51550
fragaria_vesca FvH4_7g27970
malus_domestica MD01G1184600.v1.1
prunus_persica Prupe.2G280800_v2.0.a1
pyrus_communis pycom01g19620 pycom07g22950
rosa_chinensis RchiOBHm_Chr1g0374921
rosa_laevigata RLG00000026706
rosa_multiflora Rmu_sc0003145.1_g000010
rosa_roxburghii Rroxscaffold_4G00283170
rosa_rugosa Rorug01G0386000
rosa_samantha Rh1AG397500 Rh1BG361400 Rh1CG375000 Rh1DG392400
rosa_wichuraiana Rw1G035250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 569
AccB1I GGYRCC 3 cut(s) 108, 413, 947
AccB7I CCANNNNNTGG 2 cut(s) 105, 533
AccII CGCG 8 cut(s) 271, 291, 386, 397, 460, 650, 788, 839
AclWI GGATC 2 cut(s) 964, 977
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 1 cut(s) 167
AcvI CACGTG 1 cut(s) 261
AcyI GRCGYC 2 cut(s) 109, 414
AdeI CACNNNGTG 1 cut(s) 533
AfiI CCNNNNNNNGG 3 cut(s) 105, 348, 533
AflIII ACRYGT 2 cut(s) 564, 648
AgeI ACCGGT 1 cut(s) 429
AgsI TTSAA 1 cut(s) 767
AjiI CACGTC 2 cut(s) 565, 757
AjnI CCWGG 4 cut(s) 113, 573, 679, 830
AleI CACNNNNGTG 1 cut(s) 945
AluBI AGCT 4 cut(s) 364, 409, 805, 928
AluI AGCT 4 cut(s) 364, 409, 805, 928
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 2 cut(s) 964, 977
Ama87I CYCGRG 2 cut(s) 721, 850
AoxI GGCC 4 cut(s) 116, 211, 509, 666
ApaI GGGCCC 1 cut(s) 215
ApeKI GCWGC 2 cut(s) 265, 640
ApoI RAATTY 1 cut(s) 167
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AsiGI ACCGGT 1 cut(s) 429
AspS9I GGNCC 7 cut(s) 117, 211, 212, 354, 403, 847, 1028
AsuC2I CCSGG 3 cut(s) 418, 722, 723
AsuHPI GGTGA 3 cut(s) 64, 735, 764
AsuII TTCGAA 1 cut(s) 171
AvaI CYCGRG 2 cut(s) 721, 850
AvaII GGWCC 4 cut(s) 354, 403, 847, 1028
BaeGI GKGCMC 1 cut(s) 215
BaeI ACNNNNGTAYC 3 cut(s) 34, 826, 859
BamHI GGATCC 1 cut(s) 969
BanI GGYRCC 3 cut(s) 108, 413, 947
BanII GRGCYC 2 cut(s) 215, 540
BauI CACGAG 1 cut(s) 791
BbrPI CACGTG 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 2 cut(s) 277, 627
BccI CCATC 3 cut(s) 314, 925, 938
BceAI ACGGC 6 cut(s) 271, 476, 496, 513, 628, 689
BciT130I CCWGG 4 cut(s) 115, 575, 681, 832
BciVI GTATCC 1 cut(s) 626
BclI TGATCA 1 cut(s) 480
BcnI CCSGG 3 cut(s) 418, 722, 723
BcoDI GTCTC 1 cut(s) 150
BfoI RGCGCY 4 cut(s) 112, 331, 417, 492
BfuI GTATCC 1 cut(s) 626
BglI GCCNNNNNGGC 2 cut(s) 21, 497
BisI GCNGC 5 cut(s) 266, 269, 315, 641, 734
BlpI GCTNAGC 1 cut(s) 801
BlsI GCNGC 5 cut(s) 267, 270, 316, 642, 735
Bme1390I CCNGG 7 cut(s) 115, 418, 575, 681, 722, 723, 832
Bme18I GGWCC 4 cut(s) 354, 403, 847, 1028
BmeT110I CYCGRG 2 cut(s) 721, 850
BmgBI CACGTC 2 cut(s) 565, 757
BmgT120I GGNCC 7 cut(s) 117, 211, 212, 354, 403, 847, 1028
BmrFI CCNGG 7 cut(s) 115, 418, 575, 681, 722, 723, 832
Bpu1102I GCTNAGC 1 cut(s) 801
Bpu14I TTCGAA 1 cut(s) 171
BpuMI CCSGG 3 cut(s) 418, 722, 723
BsaAI YACGTR 3 cut(s) 261, 687, 954
BsaHI GRCGYC 2 cut(s) 109, 414
BsaJI CCNNGG 3 cut(s) 113, 721, 722
BsaWI WCCGGW 4 cut(s) 46, 429, 859, 1025
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 348, 533
Bse118I RCCGGY 6 cut(s) 20, 40, 317, 341, 380, 429
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 4 cut(s) 115, 575, 681, 832
BseDI CCNNGG 3 cut(s) 113, 721, 722
BseGI GGATG 2 cut(s) 285, 777
BseLI CCNNNNNNNGG 3 cut(s) 105, 348, 533
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 2 cut(s) 47, 792
BseRI GAGGAG 2 cut(s) 50, 169
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 2 cut(s) 277, 627
BseYI CCCAGC 2 cut(s) 6, 94
Bsh1236I CGCG 8 cut(s) 271, 291, 386, 397, 460, 650, 788, 839
Bsh1285I CGRYCG 2 cut(s) 430, 864
BshFI GGCC 4 cut(s) 118, 213, 511, 668
BshNI GGYRCC 3 cut(s) 108, 413, 947
BshTI ACCGGT 1 cut(s) 429
BsiEI CGRYCG 2 cut(s) 430, 864
BsiHKAI GWGCWC 1 cut(s) 54
BsiHKCI CYCGRG 2 cut(s) 721, 850
BsiWI CGTACG 1 cut(s) 645
BslI CCNNNNNNNGG 3 cut(s) 105, 348, 533
BsmAI GTCTC 1 cut(s) 150
BsnI GGCC 4 cut(s) 118, 213, 511, 668
BsoBI CYCGRG 2 cut(s) 721, 850
Bsp119I TTCGAA 1 cut(s) 171
Bsp120I GGGCCC 1 cut(s) 211
Bsp1286I GDGCHC 3 cut(s) 54, 215, 540
Bsp143I GATC 6 cut(s) 282, 480, 874, 937, 969, 1042
Bsp1720I GCTNAGC 1 cut(s) 801
BspANI GGCC 4 cut(s) 118, 213, 511, 668
BspCNI CTCAG 2 cut(s) 46, 793
BspFNI CGCG 8 cut(s) 271, 291, 386, 397, 460, 650, 788, 839
BspPI GGATC 2 cut(s) 964, 977
BspT104I TTCGAA 1 cut(s) 171
BspT107I GGYRCC 3 cut(s) 108, 413, 947
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 6 cut(s) 20, 40, 317, 341, 380, 429
BssAI RCCGGY 6 cut(s) 20, 40, 317, 341, 380, 429
BssECI CCNNGG 3 cut(s) 113, 721, 722
BssMI GATC 6 cut(s) 282, 480, 874, 937, 969, 1042
BssNI GRCGYC 2 cut(s) 109, 414
BssSI CACGAG 1 cut(s) 791
Bst2BI CACGAG 1 cut(s) 791
Bst2UI CCWGG 4 cut(s) 115, 575, 681, 832
Bst4CI ACNGT 9 cut(s) 67, 238, 358, 403, 446, 474, 752, 896, 1022
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 2 cut(s) 109, 414
BstBAI YACGTR 3 cut(s) 261, 687, 954
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 3 cut(s) 26, 319, 807
BstDEI CTNAG 2 cut(s) 33, 801
BstF5I GGATG 2 cut(s) 285, 777
BstFNI CGCG 8 cut(s) 271, 291, 386, 397, 460, 650, 788, 839
BstH2I RGCGCY 4 cut(s) 112, 331, 417, 492
BstKTI GATC 6 cut(s) 285, 483, 877, 940, 972, 1045
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 6 cut(s) 282, 480, 874, 937, 969, 1042
BstMCI CGRYCG 2 cut(s) 430, 864
BstMWI GCNNNNNNNGC 8 cut(s) 21, 34, 95, 115, 327, 497, 806, 836
BstNI CCWGG 4 cut(s) 115, 575, 681, 832
BstSCI CCNGG 7 cut(s) 113, 416, 573, 679, 720, 721, 830
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 8 cut(s) 271, 291, 386, 397, 460, 650, 788, 839
BstV1I GCAGC 2 cut(s) 277, 627
BstX2I RGATCY 1 cut(s) 969
BstYI RGATCY 1 cut(s) 969
BsuI GTATCC 1 cut(s) 626
BsuRI GGCC 4 cut(s) 118, 213, 511, 668
BtrI CACGTC 2 cut(s) 565, 757
BtsCI GGATG 2 cut(s) 285, 777
BtsIMutI CAGTG 1 cut(s) 998
Cac8I GCNNGC 3 cut(s) 26, 319, 807
Cfr10I RCCGGY 6 cut(s) 20, 40, 317, 341, 380, 429
Cfr13I GGNCC 7 cut(s) 117, 211, 212, 354, 403, 847, 1028
Cfr9I CCCGGG 1 cut(s) 721
CseI GACGC 1 cut(s) 466
CspAI ACCGGT 1 cut(s) 429
CviAII CATG 2 cut(s) 105, 208
DdeI CTNAG 2 cut(s) 33, 801
DinI GGCGCC 2 cut(s) 110, 415
DpnI GATC 6 cut(s) 284, 482, 876, 939, 971, 1044
DpnII GATC 6 cut(s) 282, 480, 874, 937, 969, 1042
DraI TTTAAA 1 cut(s) 1014
DraIII CACNNNGTG 1 cut(s) 533
DrdI GACNNNNNNGTC 1 cut(s) 569
DseDI GACNNNNNNGTC 1 cut(s) 569
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EciI GGCGGA 1 cut(s) 76
Eco24I GRGCYC 2 cut(s) 215, 540
Eco47I GGWCC 4 cut(s) 354, 403, 847, 1028
Eco72I CACGTG 1 cut(s) 261
Eco88I CYCGRG 2 cut(s) 721, 850
EcoO109I RGGNCCY 2 cut(s) 212, 847
EcoRII CCWGG 4 cut(s) 113, 573, 679, 830
EcoT38I GRGCYC 2 cut(s) 215, 540
EgeI GGCGCC 2 cut(s) 110, 415
EheI GGCGCC 2 cut(s) 110, 415
FaeI CATG 2 cut(s) 108, 211
FaiI YATR 7 cut(s) 106, 209, 233, 250, 717, 957, 962
FatI CATG 2 cut(s) 104, 207
FauI CCCGC 2 cut(s) 832, 902
FbaI TGATCA 1 cut(s) 480
Fnu4HI GCNGC 5 cut(s) 266, 269, 315, 641, 734
FokI GGATG 2 cut(s) 272, 784
FriOI GRGCYC 2 cut(s) 215, 540
Fsp4HI GCNGC 5 cut(s) 266, 269, 315, 641, 734
GluI GCNGC 5 cut(s) 266, 269, 315, 641, 734
GsaI CCCAGC 2 cut(s) 10, 98
HaeII RGCGCY 4 cut(s) 112, 331, 417, 492
HaeIII GGCC 4 cut(s) 118, 213, 511, 668
HgaI GACGC 1 cut(s) 466
Hin1I GRCGYC 2 cut(s) 109, 414
Hin1II CATG 2 cut(s) 108, 211
HinfI GANTC 2 cut(s) 600, 888
HphI GGTGA 3 cut(s) 64, 735, 764
Hpy166II GTNNAC 4 cut(s) 241, 568, 826, 918
Hpy188I TCNGA 6 cut(s) 142, 155, 184, 559, 632, 974
Hpy188III TCNNGA 4 cut(s) 466, 845, 865, 941
Hpy8I GTNNAC 4 cut(s) 241, 568, 826, 918
Hpy99I CGWCG 3 cut(s) 341, 632, 978
HpyAV CCTTC 3 cut(s) 65, 167, 761
HpyCH4III ACNGT 9 cut(s) 67, 238, 358, 403, 446, 474, 752, 896, 1022
HpyCH4IV ACGT 6 cut(s) 260, 564, 570, 686, 756, 953
HpyCH4V TGCA 3 cut(s) 104, 585, 1037
HpyF10VI GCNNNNNNNGC 8 cut(s) 21, 34, 95, 115, 327, 497, 806, 836
HpyF3I CTNAG 2 cut(s) 33, 801
HpySE526I ACGT 6 cut(s) 260, 564, 570, 686, 756, 953
Hsp92I GRCGYC 2 cut(s) 109, 414
Hsp92II CATG 2 cut(s) 108, 211
KasI GGCGCC 2 cut(s) 108, 413
KroI GCCGGC 1 cut(s) 317
KroNI GCCGGC 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 480
Kzo9I GATC 6 cut(s) 282, 480, 874, 937, 969, 1042
LmnI GCTCC 5 cut(s) 49, 57, 182, 326, 1002
Lsp1109I GCAGC 2 cut(s) 277, 627
MaeII ACGT 6 cut(s) 260, 564, 570, 686, 756, 953
MaeIII GTNAC 5 cut(s) 493, 513, 741, 752, 812
MalI GATC 6 cut(s) 284, 482, 876, 939, 971, 1044
MboI GATC 6 cut(s) 282, 480, 874, 937, 969, 1042
MboII GAAGA 3 cut(s) 70, 289, 292
MflI RGATCY 1 cut(s) 969
MhlI GDGCHC 3 cut(s) 54, 215, 540
MluCI AATT 3 cut(s) 167, 671, 1015
MluI ACGCGT 1 cut(s) 648
Mly113I GGCGCC 2 cut(s) 109, 414
MmeI TCCRAC 3 cut(s) 165, 610, 997
MroNI GCCGGC 1 cut(s) 317
MseI TTAA 2 cut(s) 728, 1013
MslI CAYNNNNRTG 1 cut(s) 945
MspA1I CMGCKG 1 cut(s) 928
MspR9I CCNGG 7 cut(s) 115, 418, 575, 681, 722, 723, 832
MvaI CCWGG 4 cut(s) 115, 575, 681, 832
MvnI CGCG 8 cut(s) 271, 291, 386, 397, 460, 650, 788, 839
MwoI GCNNNNNNNGC 8 cut(s) 21, 34, 95, 115, 327, 497, 806, 836
NaeI GCCGGC 1 cut(s) 319
NarI GGCGCC 2 cut(s) 109, 414
NciI CCSGG 3 cut(s) 418, 722, 723
NdeII GATC 6 cut(s) 282, 480, 874, 937, 969, 1042
NgoMIV GCCGGC 1 cut(s) 317
NlaIII CATG 2 cut(s) 108, 211
NmeAIII GCCGAG 2 cut(s) 390, 644
NmuCI GTSAC 2 cut(s) 741, 752
NspV TTCGAA 1 cut(s) 171
OliI CACNNNNGTG 1 cut(s) 945
PcsI WCGNNNNNNNCGW 3 cut(s) 464, 503, 708
PdiI GCCGGC 1 cut(s) 319
PfeI GAWTC 2 cut(s) 600, 888
Pfl23II CGTACG 1 cut(s) 645
PflMI CCANNNNNTGG 2 cut(s) 105, 533
PfoI TCCNGGA 1 cut(s) 573
PinAI ACCGGT 1 cut(s) 429
PkrI GCNGC 5 cut(s) 267, 270, 316, 642, 735
PluTI GGCGCC 2 cut(s) 112, 417
PmaCI CACGTG 1 cut(s) 261
PmlI CACGTG 1 cut(s) 261
Ppu21I YACGTR 3 cut(s) 261, 687, 954
PpuMI RGGWCCY 1 cut(s) 847
Psp5II RGGWCCY 1 cut(s) 847
Psp6I CCWGG 4 cut(s) 113, 573, 679, 830
PspCI CACGTG 1 cut(s) 261
PspFI CCCAGC 2 cut(s) 6, 94
PspGI CCWGG 4 cut(s) 113, 573, 679, 830
PspLI CGTACG 1 cut(s) 645
PspOMI GGGCCC 1 cut(s) 211
PspPI GGNCC 7 cut(s) 117, 211, 212, 354, 403, 847, 1028
PspPPI RGGWCCY 1 cut(s) 847
PsrI GAACNNNNNNTAC 2 cut(s) 818, 850
PsuI RGATCY 1 cut(s) 969
PvuII CAGCTG 1 cut(s) 928
RseI CAYNNNNRTG 1 cut(s) 945
SaqAI TTAA 2 cut(s) 728, 1013
SatI GCNGC 5 cut(s) 266, 269, 315, 641, 734
Sau3AI GATC 6 cut(s) 282, 480, 874, 937, 969, 1042
Sau96I GGNCC 7 cut(s) 117, 211, 212, 354, 403, 847, 1028
ScrFI CCNGG 7 cut(s) 115, 418, 575, 681, 722, 723, 832
SduI GDGCHC 3 cut(s) 54, 215, 540
SfoI GGCGCC 2 cut(s) 110, 415
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 4 cut(s) 354, 403, 847, 1028
SmaI CCCGGG 1 cut(s) 723
SmiMI CAYNNNNRTG 1 cut(s) 945
Sse9I AATT 3 cut(s) 167, 671, 1015
SspDI GGCGCC 2 cut(s) 108, 413
StyD4I CCNGG 7 cut(s) 113, 416, 573, 679, 720, 721, 830
TaaI ACNGT 9 cut(s) 67, 238, 358, 403, 446, 474, 752, 896, 1022
TaiI ACGT 6 cut(s) 263, 567, 573, 689, 759, 956
TaqI TCGA 4 cut(s) 171, 467, 864, 1041
TasI AATT 3 cut(s) 167, 671, 1015
TatI WGTACW 1 cut(s) 217
TauI GCSGC 3 cut(s) 271, 317, 736
TfiI GAWTC 2 cut(s) 600, 888
Tru1I TTAA 2 cut(s) 728, 1013
Tru9I TTAA 2 cut(s) 728, 1013
TscAI CASTG 1 cut(s) 998
TseFI GTSAC 2 cut(s) 741, 752
TseI GCWGC 2 cut(s) 265, 640
Tsp45I GTSAC 2 cut(s) 741, 752
TspDTI ATGAA 2 cut(s) 286, 977
TspGWI ACGGA 3 cut(s) 325, 466, 991
TspMI CCCGGG 1 cut(s) 721
TspRI CASTG 1 cut(s) 998
Van91I CCANNNNNTGG 2 cut(s) 105, 533
VpaK11BI GGWCC 4 cut(s) 354, 403, 847, 1028
XapI RAATTY 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 102
XmaI CCCGGG 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.