Rw1G035250

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014954
Physical Location & Seq
Reverse (-)
63104362 .. 63105962
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035250.1

Sequence Viewer

Length: 1017 bp
ATGCGCCGTTCACCGGCTCCGTTGCTCGTCTCCGTCCTCACCCTACTCTCCCTACTCCTCTCCGCCGCTTCATGGCGGCCCTGGCCCCACCTCAAGGCCAACAACACTGACCTCGCCCTCGGCAACAACAAAAAATTCGAAGGCTCCTCCGACTTTGTCCACCTTAAATACCACATGGGGCCGGTCCTCACCGCCGACATAACCGTCCACACAATCTGGTACGGCACGTGGCAGCGCCGCCAGAAGAAGATCATTCGTGAGTTCATCAGCTCCATCTCCGCCGCCGACTCCAAGCCCCCTTCCGTCGCCGGTTGGTGGAAGACCGTACAGCTCTACACCGACCAAACCGGCCAGAACGTCTCCCGCACCGTCCACCTCGGCGCCGAGAAAAACGACCGCTTCTACTCCCACGGCAAATCCCTCACCCGCCTCTCCATCCAGTCCGTCATCAAGTCCGCCGTCACCGCCACCACCCGCCCCTTACCCACCAACCCACAAGGCGGCCTCTACCTCCTGCTGACGTCAGACGACGTCACCGTCCAGGACTTCTGCGGCCAGGTCTGCGGCTTCCACTACTTCACGTTCCCGTCCATCGTCGGCTACACTCTGCCGTACGCGTGGGTCGGGAACTCCGCGAAGCTCTGCCCCGGGGTCTGCGCGTACCCGTACGCCGTGCCGGAGTACATGCCGGGGTTGAAGCCGTTCAAGTCTCCGAACGGCGACGTCGGCGTGGAGGGGATGATAAGTGTTATTGCTCACGAGATTGCCGAGCTGGCCAGCAACCCTCTAGTGAACGCTTGGTACGCCGGTCAGGACCCCAGTTTTCCGGTGGAGATCGCCGACCTGTGCGAGGGGATCTACGGCACCGGAGGAGGGGGGTCGTACACGGGGCAGCTGATGGACGACCACGACGGCGCCACGTACAACATGAACGGAATCCGGCGCAAGTTTTTGGTGCAGTGGGTTTGGAGTCACGTTTTGAGTTACTGTACCGGACCTAATGCACTCGACCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0008150 GO:0009505 GO:0009506 GO:0009628 GO:0030054 GO:0030312 GO:0044464 GO:0050896 GO:0055044 GO:0071944 GO:0080167
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

338

Amino Acids

37.16

Weight (kDa)

8.14

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 55 - 332 5.9e-101 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51550
fragaria_vesca FvH4_7g27970
malus_domestica MD01G1184600.v1.1
prunus_persica Prupe.2G280800_v2.0.a1
pyrus_communis pycom01g19620 pycom07g22950
rosa_chinensis RchiOBHm_Chr1g0374921
rosa_laevigata RLG00000026706
rosa_multiflora Rmu_sc0003145.1_g000010
rosa_roxburghii Rroxscaffold_4G00283170
rosa_rugosa Rorug01G0386000
rosa_samantha Rh1AG397500 Rh1BG361400 Rh1CG375000 Rh1DG392400
rosa_wichuraiana Rw1G035250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 203, 536
AatII GACGTC 3 cut(s) 524, 534, 726
AccB1I GGYRCC 3 cut(s) 380, 863, 914
AccII CGCG 3 cut(s) 617, 635, 659
AclWI GGATC 1 cut(s) 863
AcoI YGGCCR 3 cut(s) 349, 553, 774
AcsI RAATTY 1 cut(s) 134
AcvI CACGTG 1 cut(s) 228
AcyI GRCGYC 5 cut(s) 381, 521, 531, 723, 915
AfiI CCNNNNNNNGG 7 cut(s) 13, 72, 94, 315, 500, 850, 873
AflIII ACRYGT 1 cut(s) 615
AgsI TTSAA 2 cut(s) 697, 706
AjnI CCWGG 3 cut(s) 80, 540, 555
AluBI AGCT 5 cut(s) 270, 331, 640, 772, 895
AluI AGCT 5 cut(s) 270, 331, 640, 772, 895
Alw26I GTCTC 3 cut(s) 34, 364, 714
AlwI GGATC 1 cut(s) 863
Ama87I CYCGRG 1 cut(s) 647
AoxI GGCC 8 cut(s) 77, 83, 96, 179, 349, 502, 553, 774
ApeKI GCWGC 2 cut(s) 232, 892
ApoI RAATTY 1 cut(s) 134
Asp700I GAANNNNTTC 1 cut(s) 701
AspLEI GCGC 6 cut(s) 6, 237, 383, 659, 917, 945
AspS9I GGNCC 6 cut(s) 78, 84, 179, 184, 814, 995
AsuC2I CCSGG 3 cut(s) 648, 649, 690
AsuHPI GGTGA 6 cut(s) 3, 31, 181, 415, 454, 526
AsuII TTCGAA 1 cut(s) 138
AvaI CYCGRG 1 cut(s) 647
AvaII GGWCC 3 cut(s) 184, 814, 995
BaeI ACNNNNGTAYC 2 cut(s) 793, 826
BalI TGGCCA 1 cut(s) 776
BanI GGYRCC 3 cut(s) 380, 863, 914
BauI CACGAG 1 cut(s) 758
BbrPI CACGTG 1 cut(s) 228
BbsI GAAGAC 1 cut(s) 326
BbvI GCAGC 2 cut(s) 244, 904
BccI CCATC 4 cut(s) 281, 443, 599, 892
BceAI ACGGC 9 cut(s) 238, 427, 443, 595, 656, 685, 733, 877, 928
BciT130I CCWGG 3 cut(s) 82, 542, 557
BcnI CCSGG 3 cut(s) 648, 649, 690
BcoDI GTCTC 3 cut(s) 34, 364, 714
BfaI CTAG 1 cut(s) 788
BfoI RGCGCY 3 cut(s) 238, 384, 918
BglI GCCNNNNNGGC 1 cut(s) 773
BisI GCNGC 9 cut(s) 66, 77, 233, 238, 282, 502, 553, 565, 893
BlsI GCNGC 9 cut(s) 67, 78, 234, 239, 283, 503, 554, 566, 894
Bme1390I CCNGG 6 cut(s) 82, 542, 557, 648, 649, 690
Bme18I GGWCC 3 cut(s) 184, 814, 995
BmeT110I CYCGRG 1 cut(s) 647
BmgT120I GGNCC 6 cut(s) 78, 84, 179, 184, 814, 995
BmiI GGNNCC 8 cut(s) 18, 86, 145, 180, 382, 816, 865, 916
BmrFI CCNGG 6 cut(s) 82, 542, 557, 648, 649, 690
BmrI ACTGGG 1 cut(s) 813
BmuI ACTGGG 1 cut(s) 813
BpiI GAAGAC 1 cut(s) 326
Bpu14I TTCGAA 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 3 cut(s) 648, 649, 690
BsaAI YACGTR 2 cut(s) 228, 921
BsaHI GRCGYC 5 cut(s) 381, 521, 531, 723, 915
BsaJI CCNNGG 8 cut(s) 80, 118, 376, 409, 646, 647, 648, 689
BsaWI WCCGGW 3 cut(s) 826, 866, 992
Bsc4I CCNNNNNNNGG 7 cut(s) 13, 72, 94, 315, 500, 850, 873
Bse118I RCCGGY 5 cut(s) 13, 181, 308, 347, 806
Bse1I ACTGG 3 cut(s) 439, 819, 1012
BseBI CCWGG 3 cut(s) 82, 542, 557
BseDI CCNNGG 8 cut(s) 80, 118, 376, 409, 646, 647, 648, 689
BseGI GGATG 2 cut(s) 435, 744
BseLI CCNNNNNNNGG 7 cut(s) 13, 72, 94, 315, 500, 850, 873
BseNI ACTGG 3 cut(s) 439, 819, 1012
BseRI GAGGAG 3 cut(s) 47, 136, 885
BseXI GCAGC 2 cut(s) 244, 904
BsgI GTGCAG 1 cut(s) 977
Bsh1236I CGCG 3 cut(s) 617, 635, 659
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 8 cut(s) 79, 85, 98, 181, 351, 504, 555, 776
BshNI GGYRCC 3 cut(s) 380, 863, 914
BsiEI CGRYCG 1 cut(s) 397
BsiHKCI CYCGRG 1 cut(s) 647
BsiWI CGTACG 2 cut(s) 612, 666
BslI CCNNNNNNNGG 7 cut(s) 13, 72, 94, 315, 500, 850, 873
BsmAI GTCTC 3 cut(s) 34, 364, 714
BsmBI CGTCTC 2 cut(s) 34, 364
BsnI GGCC 8 cut(s) 79, 85, 98, 181, 351, 504, 555, 776
BsoBI CYCGRG 1 cut(s) 647
Bsp119I TTCGAA 1 cut(s) 138
Bsp143I GATC 3 cut(s) 249, 834, 855
BspANI GGCC 8 cut(s) 79, 85, 98, 181, 351, 504, 555, 776
BspFNI CGCG 3 cut(s) 617, 635, 659
BspLI GGNNCC 8 cut(s) 18, 86, 145, 180, 382, 816, 865, 916
BspPI GGATC 1 cut(s) 863
BspT104I TTCGAA 1 cut(s) 138
BspT107I GGYRCC 3 cut(s) 380, 863, 914
BsrFI RCCGGY 5 cut(s) 13, 181, 308, 347, 806
BsrI ACTGG 3 cut(s) 439, 819, 1012
BssAI RCCGGY 5 cut(s) 13, 181, 308, 347, 806
BssECI CCNNGG 8 cut(s) 80, 118, 376, 409, 646, 647, 648, 689
BssMI GATC 3 cut(s) 249, 834, 855
BssNI GRCGYC 5 cut(s) 381, 521, 531, 723, 915
BssSI CACGAG 1 cut(s) 758
Bst2BI CACGAG 1 cut(s) 758
Bst2UI CCWGG 3 cut(s) 82, 542, 557
Bst4CI ACNGT 5 cut(s) 205, 325, 370, 538, 989
BstACI GRCGYC 5 cut(s) 381, 521, 531, 723, 915
BstBAI YACGTR 2 cut(s) 228, 921
BstBI TTCGAA 1 cut(s) 138
BstC8I GCNNGC 2 cut(s) 774, 778
BstDSI CCRYGG 1 cut(s) 409
BstENI CCTNNNNNAGG 1 cut(s) 848
BstF5I GGATG 2 cut(s) 435, 744
BstFNI CGCG 3 cut(s) 617, 635, 659
BstH2I RGCGCY 3 cut(s) 238, 384, 918
BstHHI GCGC 6 cut(s) 6, 237, 383, 659, 917, 945
BstKTI GATC 3 cut(s) 252, 837, 858
BstMAI GTCTC 3 cut(s) 34, 364, 714
BstMBI GATC 3 cut(s) 249, 834, 855
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 6 cut(s) 82, 464, 561, 726, 773, 803
BstNI CCWGG 3 cut(s) 82, 542, 557
BstNSI RCATGY 1 cut(s) 688
BstSCI CCNGG 6 cut(s) 80, 540, 555, 646, 647, 688
BstUI CGCG 3 cut(s) 617, 635, 659
BstV1I GCAGC 2 cut(s) 244, 904
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 1 cut(s) 855
BstYI RGATCY 1 cut(s) 855
BsuRI GGCC 8 cut(s) 79, 85, 98, 181, 351, 504, 555, 776
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 2 cut(s) 435, 744
BtsI GCAGTG 1 cut(s) 965
BtsIMutI CAGTG 2 cut(s) 105, 965
Cac8I GCNNGC 2 cut(s) 774, 778
CfoI GCGC 6 cut(s) 6, 237, 383, 659, 917, 945
Cfr10I RCCGGY 5 cut(s) 13, 181, 308, 347, 806
Cfr13I GGNCC 6 cut(s) 78, 84, 179, 184, 814, 995
Cfr9I CCCGGG 1 cut(s) 647
CviAII CATG 4 cut(s) 72, 175, 685, 928
DinI GGCGCC 2 cut(s) 382, 916
DpnI GATC 3 cut(s) 251, 836, 857
DpnII GATC 3 cut(s) 249, 834, 855
DrdI GACNNNNNNGTC 2 cut(s) 203, 536
DseDI GACNNNNNNGTC 2 cut(s) 203, 536
EaeI YGGCCR 3 cut(s) 349, 553, 774
EciI GGCGGA 3 cut(s) 52, 268, 445
Eco47I GGWCC 3 cut(s) 184, 814, 995
Eco72I CACGTG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 647
EcoNI CCTNNNNNAGG 1 cut(s) 848
EcoO109I RGGNCCY 1 cut(s) 814
EcoRII CCWGG 3 cut(s) 80, 540, 555
EgeI GGCGCC 2 cut(s) 382, 916
EheI GGCGCC 2 cut(s) 382, 916
Esp3I CGTCTC 2 cut(s) 34, 364
FaeI CATG 4 cut(s) 75, 178, 688, 931
FaiI YATR 5 cut(s) 73, 176, 200, 686, 929
FatI CATG 4 cut(s) 71, 174, 684, 927
FauI CCCGC 3 cut(s) 371, 434, 482
Fnu4HI GCNGC 9 cut(s) 66, 77, 233, 238, 282, 502, 553, 565, 893
FokI GGATG 2 cut(s) 422, 751
Fsp4HI GCNGC 9 cut(s) 66, 77, 233, 238, 282, 502, 553, 565, 893
FspBI CTAG 1 cut(s) 788
GlaI GCGC 6 cut(s) 5, 236, 382, 658, 916, 944
GluI GCNGC 9 cut(s) 66, 77, 233, 238, 282, 502, 553, 565, 893
HaeII RGCGCY 3 cut(s) 238, 384, 918
HaeIII GGCC 8 cut(s) 79, 85, 98, 181, 351, 504, 555, 776
HhaI GCGC 6 cut(s) 6, 237, 383, 659, 917, 945
Hin1I GRCGYC 5 cut(s) 381, 521, 531, 723, 915
Hin1II CATG 4 cut(s) 75, 178, 688, 931
Hin6I GCGC 6 cut(s) 4, 235, 381, 657, 915, 943
HinP1I GCGC 6 cut(s) 4, 235, 381, 657, 915, 943
HinfI GANTC 3 cut(s) 287, 936, 970
HphI GGTGA 6 cut(s) 3, 31, 181, 415, 454, 526
Hpy166II GTNNAC 6 cut(s) 11, 160, 208, 373, 793, 885
Hpy188I TCNGA 3 cut(s) 151, 526, 714
Hpy188III TCNNGA 4 cut(s) 257, 625, 758, 812
Hpy8I GTNNAC 6 cut(s) 11, 160, 208, 373, 793, 885
Hpy99I CGWCG 6 cut(s) 308, 533, 599, 725, 728, 914
HpyAV CCTTC 2 cut(s) 134, 309
HpyCH4III ACNGT 5 cut(s) 205, 325, 370, 538, 989
HpyCH4IV ACGT 8 cut(s) 227, 357, 521, 531, 581, 723, 920, 975
HpyCH4V TGCA 2 cut(s) 958, 1004
HpyF10VI GCNNNNNNNGC 6 cut(s) 82, 464, 561, 726, 773, 803
HpySE526I ACGT 8 cut(s) 227, 357, 521, 531, 581, 723, 920, 975
Hsp92I GRCGYC 5 cut(s) 381, 521, 531, 723, 915
Hsp92II CATG 4 cut(s) 75, 178, 688, 931
HspAI GCGC 6 cut(s) 4, 235, 381, 657, 915, 943
KasI GGCGCC 2 cut(s) 380, 914
Kzo9I GATC 3 cut(s) 249, 834, 855
LmnI GCTCC 3 cut(s) 22, 149, 275
Lsp1109I GCAGC 2 cut(s) 244, 904
MaeI CTAG 1 cut(s) 788
MaeII ACGT 8 cut(s) 227, 357, 521, 531, 581, 723, 920, 975
MaeIII GTNAC 4 cut(s) 460, 532, 971, 983
MalI GATC 3 cut(s) 251, 836, 857
MboI GATC 3 cut(s) 249, 834, 855
MboII GAAGA 3 cut(s) 256, 259, 331
MflI RGATCY 1 cut(s) 855
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 1 cut(s) 134
MluI ACGCGT 1 cut(s) 615
MluNI TGGCCA 1 cut(s) 776
Mly113I GGCGCC 2 cut(s) 381, 915
MlyI GAGTC 2 cut(s) 281, 979
MmeI TCCRAC 1 cut(s) 174
Mox20I TGGCCA 1 cut(s) 776
MroXI GAANNNNTTC 1 cut(s) 701
MscI TGGCCA 1 cut(s) 776
MseI TTAA 1 cut(s) 165
Msp20I TGGCCA 1 cut(s) 776
MspA1I CMGCKG 1 cut(s) 895
MspR9I CCNGG 6 cut(s) 82, 542, 557, 648, 649, 690
MvaI CCWGG 3 cut(s) 82, 542, 557
MvnI CGCG 3 cut(s) 617, 635, 659
MwoI GCNNNNNNNGC 6 cut(s) 82, 464, 561, 726, 773, 803
NarI GGCGCC 2 cut(s) 381, 915
NciI CCSGG 3 cut(s) 648, 649, 690
NdeII GATC 3 cut(s) 249, 834, 855
NlaIII CATG 4 cut(s) 75, 178, 688, 931
NlaIV GGNNCC 8 cut(s) 18, 86, 145, 180, 382, 816, 865, 916
NmeAIII GCCGAG 4 cut(s) 99, 357, 409, 793
NmuCI GTSAC 3 cut(s) 460, 532, 971
NspI RCATGY 1 cut(s) 688
NspV TTCGAA 1 cut(s) 138
PcsI WCGNNNNNNNCGW 2 cut(s) 534, 765
PdmI GAANNNNTTC 1 cut(s) 701
PfeI GAWTC 1 cut(s) 936
Pfl23II CGTACG 2 cut(s) 612, 666
PflFI GACNNNGTC 2 cut(s) 155, 530
PfoI TCCNGGA 1 cut(s) 540
PkrI GCNGC 9 cut(s) 67, 78, 234, 239, 283, 503, 554, 566, 894
PleI GAGTC 2 cut(s) 281, 978
PluTI GGCGCC 2 cut(s) 384, 918
PmaCI CACGTG 1 cut(s) 228
PmlI CACGTG 1 cut(s) 228
PpsI GAGTC 2 cut(s) 281, 978
Ppu21I YACGTR 2 cut(s) 228, 921
PpuMI RGGWCCY 1 cut(s) 814
Psp5II RGGWCCY 1 cut(s) 814
Psp6I CCWGG 3 cut(s) 80, 540, 555
PspCI CACGTG 1 cut(s) 228
PspGI CCWGG 3 cut(s) 80, 540, 555
PspLI CGTACG 2 cut(s) 612, 666
PspN4I GGNNCC 8 cut(s) 18, 86, 145, 180, 382, 816, 865, 916
PspPI GGNCC 6 cut(s) 78, 84, 179, 184, 814, 995
PspPPI RGGWCCY 1 cut(s) 814
PsrI GAACNNNNNNTAC 2 cut(s) 785, 817
PsuI RGATCY 1 cut(s) 855
PsyI GACNNNGTC 2 cut(s) 155, 530
PvuII CAGCTG 1 cut(s) 895
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 9 cut(s) 66, 77, 233, 238, 282, 502, 553, 565, 893
Sau3AI GATC 3 cut(s) 249, 834, 855
Sau96I GGNCC 6 cut(s) 78, 84, 179, 184, 814, 995
SchI GAGTC 2 cut(s) 281, 979
ScrFI CCNGG 6 cut(s) 82, 542, 557, 648, 649, 690
SfoI GGCGCC 2 cut(s) 382, 916
SfuI TTCGAA 1 cut(s) 138
SinI GGWCC 3 cut(s) 184, 814, 995
SmaI CCCGGG 1 cut(s) 649
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 1 cut(s) 134
SspDI GGCGCC 2 cut(s) 380, 914
SspMI CTAG 1 cut(s) 788
StyD4I CCNGG 6 cut(s) 80, 540, 555, 646, 647, 688
TaaI ACNGT 5 cut(s) 205, 325, 370, 538, 989
TaiI ACGT 8 cut(s) 230, 360, 524, 534, 584, 726, 923, 978
TaqI TCGA 2 cut(s) 138, 1008
TasI AATT 1 cut(s) 134
TatI WGTACW 1 cut(s) 681
TauI GCSGC 7 cut(s) 68, 79, 240, 284, 504, 555, 567
TfiI GAWTC 1 cut(s) 936
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 2 cut(s) 112, 965
TseFI GTSAC 3 cut(s) 460, 532, 971
TseI GCWGC 2 cut(s) 232, 892
Tsp45I GTSAC 3 cut(s) 460, 532, 971
TspDTI ATGAA 3 cut(s) 60, 253, 944
TspGWI ACGGA 5 cut(s) 9, 22, 292, 433, 948
TspMI CCCGGG 1 cut(s) 647
TspRI CASTG 2 cut(s) 112, 965
Tth111I GACNNNGTC 2 cut(s) 155, 530
VpaK11BI GGWCC 3 cut(s) 184, 814, 995
XagI CCTNNNNNAGG 1 cut(s) 848
XapI RAATTY 1 cut(s) 134
XceI RCATGY 1 cut(s) 688
XcmI CCANNNNNNNNNTGG 1 cut(s) 826
XmaI CCCGGG 1 cut(s) 647
XmnI GAANNNNTTC 1 cut(s) 701
XspI CTAG 1 cut(s) 788
ZraI GACGTC 3 cut(s) 522, 532, 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.