Rroxscaffold_4G00283170

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014954
Physical Location & Seq
Forward (+)
5160984 .. 5161880
897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283170.1

Sequence Viewer

Length: 897 bp
ATGCGCGGGTCACCGGCTCCGTTGCTCCTTTCCCTCCTCACCCTACTCTCCCTACTCCTCTCCGCCGCTTCATGGAGGCCCTGGCCCCACCTCAAGGCCAACAACACCGACCTTGTCCTTGGCAACAACAAAAAATTCGAAGGCTCCTCCGACTTTGTCCACCTTAAATACCACATGGGGCCGGTCCTCACCGCCGACATAACCGTCCACACAATCTGGTACGGCAGGTGGCAGCGCCGCCAGAAGAAGATCATTCGTGAGTTCATCAGCTCCATCTCCGCCGCAGACTCCAAGCCCCCTTCCGTCGCCGGTTGGTGGAAGACCGTACAGCTCTACACCGACCAAACCGGCCAGAACGTCTCCCGCACCGTCCACCTCGGCGCCGAGAAAAACGACCGCTTCTACTCCCACGGCAAATCCCTCACCCGCCTCTCCATCCAGTCCGTCATCAAGTCCGCCGTCACCGCCACCACCCGCCCCTTACCCACCAACCCACAAGGCGGCCTCTACCTCCTGCTGACGTCAGACGACGTCACCGTCCAGGACTTCTGCGGGCAGGTCTGCGGCTTCCACTACTTCACGTTCCCGTCCATCGTCGGCTACACTCTGCCGTACGCGTGGGTCGGGAACTCCGCGAAGCTCTGCCCCGGCGTCTGCGCGTACCCGTACGCCGTGCCGGAGTACATGCCGGGGTTGAAGCCGTTCAAGTCTCCGAACGGCGACGTCGGCGTGGAGGGGATGATAAGTGTTATAGCTCACGAGATTGCCGAGCTGGCGAGCAACCCTCTAGTGAACGCTTGGTACGCCGGTCAGGACCCCAGTTTTCCGGTGGAGATCGCCGACCTCTGCGAGGGGATCTACGGCACCGGAGGAGGGGGTCGTACACGGGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0008150 GO:0009505 GO:0009506 GO:0009628 GO:0030054 GO:0030312 GO:0044464 GO:0050896 GO:0055044 GO:0071944 GO:0080167
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

298

Amino Acids

32.56

Weight (kDa)

8.87

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 55 - 293 3e-86 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51550
fragaria_vesca FvH4_7g27970
malus_domestica MD01G1184600.v1.1
prunus_persica Prupe.2G280800_v2.0.a1
pyrus_communis pycom01g19620 pycom07g22950
rosa_chinensis RchiOBHm_Chr1g0374921
rosa_laevigata RLG00000026706
rosa_multiflora Rmu_sc0003145.1_g000010
rosa_roxburghii Rroxscaffold_4G00283170
rosa_rugosa Rorug01G0386000
rosa_samantha Rh1AG397500 Rh1BG361400 Rh1CG375000 Rh1DG392400
rosa_wichuraiana Rw1G035250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 216
AasI GACNNNNNNGTC 2 cut(s) 203, 536
AatII GACGTC 3 cut(s) 524, 534, 726
Acc36I ACCTGC 2 cut(s) 216, 547
AccB1I GGYRCC 2 cut(s) 380, 863
AccII CGCG 4 cut(s) 6, 617, 635, 659
AclWI GGATC 1 cut(s) 863
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 1 cut(s) 134
AcyI GRCGYC 5 cut(s) 381, 521, 531, 651, 723
AfaI GTAC 8 cut(s) 221, 327, 614, 662, 668, 683, 803, 883
AfiI CCNNNNNNNGG 6 cut(s) 72, 94, 315, 500, 850, 873
AflIII ACRYGT 1 cut(s) 615
AgsI TTSAA 2 cut(s) 697, 706
AjnI CCWGG 2 cut(s) 80, 540
AluBI AGCT 6 cut(s) 270, 331, 640, 755, 772, 894
AluI AGCT 6 cut(s) 270, 331, 640, 755, 772, 894
Alw26I GTCTC 2 cut(s) 364, 714
AlwI GGATC 1 cut(s) 863
AoxI GGCC 6 cut(s) 77, 83, 96, 179, 349, 502
ApeKI GCWGC 2 cut(s) 232, 891
ApoI RAATTY 1 cut(s) 134
Asp700I GAANNNNTTC 1 cut(s) 701
AspLEI GCGC 4 cut(s) 6, 237, 383, 659
AspS9I GGNCC 5 cut(s) 78, 84, 179, 184, 814
AsuC2I CCSGG 2 cut(s) 648, 690
AsuHPI GGTGA 6 cut(s) 3, 31, 181, 415, 454, 526
AsuII TTCGAA 1 cut(s) 138
AvaII GGWCC 2 cut(s) 184, 814
BaeI ACNNNNGTAYC 2 cut(s) 793, 826
BanI GGYRCC 2 cut(s) 380, 863
BauI CACGAG 1 cut(s) 758
BbsI GAAGAC 1 cut(s) 326
BbvI GCAGC 1 cut(s) 244
BccI CCATC 3 cut(s) 281, 443, 599
BceAI ACGGC 8 cut(s) 238, 427, 443, 595, 656, 685, 733, 877
BciT130I CCWGG 2 cut(s) 82, 542
BcnI CCSGG 2 cut(s) 648, 690
BcoDI GTCTC 2 cut(s) 364, 714
BfaI CTAG 1 cut(s) 788
BfoI RGCGCY 2 cut(s) 238, 384
BfuAI ACCTGC 2 cut(s) 216, 547
BglI GCCNNNNNGGC 1 cut(s) 773
BisI GCNGC 7 cut(s) 66, 233, 238, 282, 502, 565, 892
BlsI GCNGC 7 cut(s) 67, 234, 239, 283, 503, 566, 893
Bme1390I CCNGG 4 cut(s) 82, 542, 648, 690
Bme18I GGWCC 2 cut(s) 184, 814
BmgT120I GGNCC 5 cut(s) 78, 84, 179, 184, 814
BmiI GGNNCC 7 cut(s) 18, 86, 145, 180, 382, 816, 865
BmrFI CCNGG 4 cut(s) 82, 542, 648, 690
BmrI ACTGGG 1 cut(s) 813
BmuI ACTGGG 1 cut(s) 813
BpiI GAAGAC 1 cut(s) 326
BplI GAGNNNNNCTC 2 cut(s) 769, 801
Bpu14I TTCGAA 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 2 cut(s) 648, 690
BsaHI GRCGYC 5 cut(s) 381, 521, 531, 651, 723
BsaJI CCNNGG 6 cut(s) 80, 118, 376, 409, 646, 689
BsaWI WCCGGW 2 cut(s) 826, 866
Bsc4I CCNNNNNNNGG 6 cut(s) 72, 94, 315, 500, 850, 873
Bse118I RCCGGY 5 cut(s) 13, 181, 308, 347, 806
Bse1I ACTGG 2 cut(s) 439, 819
BseBI CCWGG 2 cut(s) 82, 542
BseDI CCNNGG 6 cut(s) 80, 118, 376, 409, 646, 689
BseGI GGATG 2 cut(s) 435, 744
BseLI CCNNNNNNNGG 6 cut(s) 72, 94, 315, 500, 850, 873
BseNI ACTGG 2 cut(s) 439, 819
BseRI GAGGAG 4 cut(s) 26, 47, 136, 885
BseXI GCAGC 1 cut(s) 244
Bsh1236I CGCG 4 cut(s) 6, 617, 635, 659
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 6 cut(s) 79, 85, 98, 181, 351, 504
BshNI GGYRCC 2 cut(s) 380, 863
BsiEI CGRYCG 1 cut(s) 397
BsiWI CGTACG 2 cut(s) 612, 666
BslI CCNNNNNNNGG 6 cut(s) 72, 94, 315, 500, 850, 873
BsmAI GTCTC 2 cut(s) 364, 714
BsmBI CGTCTC 1 cut(s) 364
BsnI GGCC 6 cut(s) 79, 85, 98, 181, 351, 504
Bsp119I TTCGAA 1 cut(s) 138
Bsp143I GATC 3 cut(s) 249, 834, 855
BspANI GGCC 6 cut(s) 79, 85, 98, 181, 351, 504
BspFNI CGCG 4 cut(s) 6, 617, 635, 659
BspLI GGNNCC 7 cut(s) 18, 86, 145, 180, 382, 816, 865
BspMI ACCTGC 2 cut(s) 216, 547
BspPI GGATC 1 cut(s) 863
BspT104I TTCGAA 1 cut(s) 138
BspT107I GGYRCC 2 cut(s) 380, 863
BsrFI RCCGGY 5 cut(s) 13, 181, 308, 347, 806
BsrI ACTGG 2 cut(s) 439, 819
BssAI RCCGGY 5 cut(s) 13, 181, 308, 347, 806
BssECI CCNNGG 6 cut(s) 80, 118, 376, 409, 646, 689
BssMI GATC 3 cut(s) 249, 834, 855
BssNI GRCGYC 5 cut(s) 381, 521, 531, 651, 723
BssSI CACGAG 1 cut(s) 758
BssT1I CCWWGG 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 758
Bst2UI CCWGG 2 cut(s) 82, 542
Bst4CI ACNGT 4 cut(s) 205, 325, 370, 538
BstACI GRCGYC 5 cut(s) 381, 521, 531, 651, 723
BstBI TTCGAA 1 cut(s) 138
BstC8I GCNNGC 3 cut(s) 554, 774, 778
BstDSI CCRYGG 1 cut(s) 409
BstEII GGTNACC 1 cut(s) 9
BstENI CCTNNNNNAGG 1 cut(s) 848
BstF5I GGATG 2 cut(s) 435, 744
BstFNI CGCG 4 cut(s) 6, 617, 635, 659
BstH2I RGCGCY 2 cut(s) 238, 384
BstHHI GCGC 4 cut(s) 6, 237, 383, 659
BstKTI GATC 3 cut(s) 252, 837, 858
BstMAI GTCTC 2 cut(s) 364, 714
BstMBI GATC 3 cut(s) 249, 834, 855
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 4 cut(s) 464, 726, 773, 803
BstNI CCWGG 2 cut(s) 82, 542
BstNSI RCATGY 1 cut(s) 688
BstPI GGTNACC 1 cut(s) 9
BstSCI CCNGG 4 cut(s) 80, 540, 646, 688
BstUI CGCG 4 cut(s) 6, 617, 635, 659
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 1 cut(s) 855
BstYI RGATCY 1 cut(s) 855
BsuRI GGCC 6 cut(s) 79, 85, 98, 181, 351, 504
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 2 cut(s) 435, 744
BveI ACCTGC 2 cut(s) 216, 547
Cac8I GCNNGC 3 cut(s) 554, 774, 778
CfoI GCGC 4 cut(s) 6, 237, 383, 659
Cfr10I RCCGGY 5 cut(s) 13, 181, 308, 347, 806
Cfr13I GGNCC 5 cut(s) 78, 84, 179, 184, 814
CseI GACGC 1 cut(s) 640
Csp6I GTAC 8 cut(s) 220, 326, 613, 661, 667, 682, 802, 882
CviAII CATG 3 cut(s) 72, 175, 685
CviQI GTAC 8 cut(s) 220, 326, 613, 661, 667, 682, 802, 882
DinI GGCGCC 1 cut(s) 382
DpnI GATC 3 cut(s) 251, 836, 857
DpnII GATC 3 cut(s) 249, 834, 855
DrdI GACNNNNNNGTC 2 cut(s) 203, 536
DseDI GACNNNNNNGTC 2 cut(s) 203, 536
EaeI YGGCCR 1 cut(s) 349
EciI GGCGGA 3 cut(s) 52, 268, 445
Eco130I CCWWGG 1 cut(s) 118
Eco47I GGWCC 2 cut(s) 184, 814
Eco91I GGTNACC 1 cut(s) 9
EcoNI CCTNNNNNAGG 1 cut(s) 848
EcoO109I RGGNCCY 2 cut(s) 78, 814
EcoO65I GGTNACC 1 cut(s) 9
EcoRII CCWGG 2 cut(s) 80, 540
EcoT14I CCWWGG 1 cut(s) 118
EgeI GGCGCC 1 cut(s) 382
EheI GGCGCC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 118
Esp3I CGTCTC 1 cut(s) 364
FaeI CATG 3 cut(s) 75, 178, 688
FaiI YATR 5 cut(s) 73, 176, 200, 686, 752
FatI CATG 3 cut(s) 71, 174, 684
FauI CCCGC 4 cut(s) 371, 434, 482, 545
Fnu4HI GCNGC 7 cut(s) 66, 233, 238, 282, 502, 565, 892
FokI GGATG 2 cut(s) 422, 751
Fsp4HI GCNGC 7 cut(s) 66, 233, 238, 282, 502, 565, 892
FspBI CTAG 1 cut(s) 788
GlaI GCGC 4 cut(s) 5, 236, 382, 658
GluI GCNGC 7 cut(s) 66, 233, 238, 282, 502, 565, 892
HaeII RGCGCY 2 cut(s) 238, 384
HaeIII GGCC 6 cut(s) 79, 85, 98, 181, 351, 504
HgaI GACGC 1 cut(s) 640
HhaI GCGC 4 cut(s) 6, 237, 383, 659
Hin1I GRCGYC 5 cut(s) 381, 521, 531, 651, 723
Hin1II CATG 3 cut(s) 75, 178, 688
Hin6I GCGC 4 cut(s) 4, 235, 381, 657
HinP1I GCGC 4 cut(s) 4, 235, 381, 657
HinfI GANTC 1 cut(s) 287
HphI GGTGA 6 cut(s) 3, 31, 181, 415, 454, 526
Hpy166II GTNNAC 5 cut(s) 160, 208, 373, 793, 884
Hpy188I TCNGA 3 cut(s) 151, 526, 714
Hpy188III TCNNGA 4 cut(s) 257, 625, 758, 812
Hpy8I GTNNAC 5 cut(s) 160, 208, 373, 793, 884
Hpy99I CGWCG 5 cut(s) 308, 533, 599, 725, 728
HpyAV CCTTC 2 cut(s) 134, 309
HpyCH4III ACNGT 4 cut(s) 205, 325, 370, 538
HpyCH4IV ACGT 5 cut(s) 357, 521, 531, 581, 723
HpyF10VI GCNNNNNNNGC 4 cut(s) 464, 726, 773, 803
HpySE526I ACGT 5 cut(s) 357, 521, 531, 581, 723
Hsp92I GRCGYC 5 cut(s) 381, 521, 531, 651, 723
Hsp92II CATG 3 cut(s) 75, 178, 688
HspAI GCGC 4 cut(s) 4, 235, 381, 657
KasI GGCGCC 1 cut(s) 380
Kzo9I GATC 3 cut(s) 249, 834, 855
LmnI GCTCC 4 cut(s) 22, 30, 149, 275
Lsp1109I GCAGC 1 cut(s) 244
MaeI CTAG 1 cut(s) 788
MaeII ACGT 5 cut(s) 357, 521, 531, 581, 723
MaeIII GTNAC 3 cut(s) 9, 460, 532
MalI GATC 3 cut(s) 251, 836, 857
MboI GATC 3 cut(s) 249, 834, 855
MboII GAAGA 3 cut(s) 256, 259, 331
MflI RGATCY 1 cut(s) 855
MluCI AATT 1 cut(s) 134
MluI ACGCGT 1 cut(s) 615
Mly113I GGCGCC 1 cut(s) 381
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 701
MseI TTAA 1 cut(s) 165
MspA1I CMGCKG 1 cut(s) 894
MspR9I CCNGG 4 cut(s) 82, 542, 648, 690
MvaI CCWGG 2 cut(s) 82, 542
MvnI CGCG 4 cut(s) 6, 617, 635, 659
MwoI GCNNNNNNNGC 4 cut(s) 464, 726, 773, 803
NarI GGCGCC 1 cut(s) 381
NciI CCSGG 2 cut(s) 648, 690
NdeII GATC 3 cut(s) 249, 834, 855
NlaIII CATG 3 cut(s) 75, 178, 688
NlaIV GGNNCC 7 cut(s) 18, 86, 145, 180, 382, 816, 865
NmeAIII GCCGAG 3 cut(s) 357, 409, 793
NmuCI GTSAC 3 cut(s) 9, 460, 532
NspI RCATGY 1 cut(s) 688
NspV TTCGAA 1 cut(s) 138
PaqCI CACCTGC 1 cut(s) 216
PcsI WCGNNNNNNNCGW 2 cut(s) 534, 765
PdmI GAANNNNTTC 1 cut(s) 701
Pfl23II CGTACG 2 cut(s) 612, 666
PflFI GACNNNGTC 3 cut(s) 113, 155, 530
PfoI TCCNGGA 1 cut(s) 540
PkrI GCNGC 7 cut(s) 67, 234, 239, 283, 503, 566, 893
PleI GAGTC 1 cut(s) 281
PluTI GGCGCC 1 cut(s) 384
PpsI GAGTC 1 cut(s) 281
PpuMI RGGWCCY 1 cut(s) 814
Psp5II RGGWCCY 1 cut(s) 814
Psp6I CCWGG 2 cut(s) 80, 540
PspEI GGTNACC 1 cut(s) 9
PspGI CCWGG 2 cut(s) 80, 540
PspLI CGTACG 2 cut(s) 612, 666
PspN4I GGNNCC 7 cut(s) 18, 86, 145, 180, 382, 816, 865
PspPI GGNCC 5 cut(s) 78, 84, 179, 184, 814
PspPPI RGGWCCY 1 cut(s) 814
PsrI GAACNNNNNNTAC 2 cut(s) 785, 817
PsuI RGATCY 1 cut(s) 855
PsyI GACNNNGTC 3 cut(s) 113, 155, 530
PvuII CAGCTG 1 cut(s) 894
RsaI GTAC 8 cut(s) 221, 327, 614, 662, 668, 683, 803, 883
RsaNI GTAC 8 cut(s) 220, 326, 613, 661, 667, 682, 802, 882
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 7 cut(s) 66, 233, 238, 282, 502, 565, 892
Sau3AI GATC 3 cut(s) 249, 834, 855
Sau96I GGNCC 5 cut(s) 78, 84, 179, 184, 814
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 4 cut(s) 82, 542, 648, 690
SfoI GGCGCC 1 cut(s) 382
SfuI TTCGAA 1 cut(s) 138
SinI GGWCC 2 cut(s) 184, 814
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 1 cut(s) 134
SspDI GGCGCC 1 cut(s) 380
SspMI CTAG 1 cut(s) 788
StyD4I CCNGG 4 cut(s) 80, 540, 646, 688
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 4 cut(s) 205, 325, 370, 538
TaiI ACGT 5 cut(s) 360, 524, 534, 584, 726
TaqI TCGA 1 cut(s) 138
TasI AATT 1 cut(s) 134
TatI WGTACW 1 cut(s) 681
TauI GCSGC 5 cut(s) 68, 240, 284, 504, 567
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TseFI GTSAC 3 cut(s) 9, 460, 532
TseI GCWGC 2 cut(s) 232, 891
Tsp45I GTSAC 3 cut(s) 9, 460, 532
TspDTI ATGAA 2 cut(s) 60, 253
TspGWI ACGGA 3 cut(s) 9, 292, 433
Tth111I GACNNNGTC 3 cut(s) 113, 155, 530
VpaK11BI GGWCC 2 cut(s) 184, 814
XagI CCTNNNNNAGG 1 cut(s) 848
XapI RAATTY 1 cut(s) 134
XceI RCATGY 1 cut(s) 688
XcmI CCANNNNNNNNNTGG 1 cut(s) 826
XmnI GAANNNNTTC 1 cut(s) 701
XspI CTAG 1 cut(s) 788
ZraI GACGTC 3 cut(s) 522, 532, 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.