MD01G1194300.v1.1

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010788
Physical Location & Seq
Reverse (-)
29313749 .. 29316765
3017 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1194300.v1.1.491

Sequence Viewer

Length: 1737 bp
ATGTGTACCACATTTTTTGGAGCTTATCTTCTTGCTGGAATTCTCTTGTTGCTTCCCATTTGTTATTCGGATAAAGCTCCGGACTATACATTTGTTAGAGAAGCAATATCAGCCCCAGCAGTGGTTCACTATGACTACATAGTCATTGGTGGAGGCACCGCAGGGTGTCCGCTAGCGGCCACTCTCTCTCACGGTGCCACCGTTTTAGTCCTGGAAAGAGGCGGCTCGCCTTATGGCAATCCGAACATAATCAACATTGACAAATTTGCCCCCACTCTTTTGGACACCTCTCCCACATCCCCAGCCCAGCAGTTCACCTCCGAGGATGGCGTCTATAACATCCGGGCACGCGTGCTGGGTGGCGGCTCAGCAGTGAATGCTGGGTTTTACACGCGTGCCAGCACACATTACATCAAGGAAGTAGGTTGGAACCAGAGAATGGTCAATCAGTCGTATGAATGGGTTGAGAAAGTGGTGGCTTTTGAACCGGAGATTTTGCAGTGGGAGACTGCTTTCAGAGATGGTTTGCTTGAAGTGGGAGTCTTGCCTCACAATAGGTTTACCTATGAGCATTTGTATGGGACTAAAGTTGGTGGGAGTATTTTTGATGCAGATGGACATAGACACACAGCTGCAGATTTGCTTCAGTATGCAGACCCTAGAAAGATCAATGTTTATTTGAATGCACGTGTACAAAAAATCTTGTTTAGACACATTCCTGGAAGACTAAGACCACAAGCTTATGGTGTGATTTATAGAGATGCACACGGTATTAGGCACTATGCATACCTAAAAATGAACTCCAAGAAGAATGAAATTATCTTATCAGCCGGTGCGATTGGGAGCCCTCAACTATTGATGCTGAGCGGCGTGGGGCCCGCCTTCCATCTCCGAGCTCACGGCATCAAGGTGGTAGCAGACCAACCCATGGTCGGCCAGGGAATGGCGGATAATCCAATGAATCTTCTGCTCATTCCCTCACCTCAACCAGTTGAGGTCTCCCTGGTTCAAGTTGTGGGCATCACAAAGTTTGAAAGCTACATTGAAGGAGCTAGCGGATTGACCCTTCCCATTCCTCTGGCTCATAGGCTTTCCAATCACTTTAAGCACTTCTTGAGTCAGCCTGAGCACCATCCTTTTAGAGCTTCACCAAAAATCATGGCTTGGGCAGCTGATACTGTAAACGGAATTGTCAATAAAACTCTCAGAGCCGGAGTCATACTTGAAAAAATCATGGGTCCACTCTCCACAGGCCATCTTGCGCTGAGGAACACAAACCCTGATGCCAATCCATTTGTCACCTTTAACTACTTCAAAGAACCAGAAGACTTAAGGAAGTGCATTGAGGGCATGAGGACCATTATCGATGTTGTGAACTCCGAAGCCTACTCAAAGTTCCGTTACAAAAACATGCCGGTTGAAGCTCTCATTAACCTGATGTTGACTTTACCAGTTAACGGAAGGCGAAAGCATGCCAACGCTACATTTTCTTTGGAACAATTCTGCATAGACACTGTGATGACCATATGGCACTATCATGGAGGTTGTCAAGTTGGAAGGGTTGTTGATAAAGGATATAGGGTTCTTGGTATTGATTCTCTTCGGGTTGTTGATGGATCGACGTTTTATCGCAGCCCGGGTACTAATCCTCAAGCTACTGTTATGATGCTCGGAAGGTACATGGGACAAAGGATTCTGCATGACAGATTACTCCGTGGATCGAAAAAGAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

579

Amino Acids

64.04

Weight (kDa)

9.27

Isoelectric Point (pI)

38.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 44 - 319 5.8e-19 GMC oxidoreductase
GMC_oxred_C PF05199 414 - 558 2e-25 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010788)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56060 AT5G51930 AT5G51930 AT5G51950 AT5G51950 AT5G51950
fragaria_vesca FvH4_7g28850
malus_domestica MD01G1194300.v1.1
prunus_persica Prupe.2G288400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0376521
rosa_laevigata RLG00000026604
rosa_multiflora Rmu_sc0001308.1_g000024 Rmu_sc0016049.1_g000002
rosa_roxburghii Rroxscaffold_4G00282040
rosa_rugosa Rorug01G0396200
rosa_samantha Rh1AG408000 Rh1BG372000 Rh1CG385800 Rh1DG403000
rosa_wichuraiana Rw1G036150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
AccB1I GGYRCC 2 cut(s) 155, 194
AccB7I CCANNNNNTGG 3 cut(s) 439, 928, 943
AccBSI CCGCTC 1 cut(s) 867
AccII CGCG 2 cut(s) 351, 394
AccIII TCCGGA 1 cut(s) 79
AciI CCGC 9 cut(s) 159, 170, 176, 222, 363, 867, 879, 947, 1056
AclWI GGATC 2 cut(s) 1624, 1726
AcoI YGGCCR 2 cut(s) 177, 934
AcsI RAATTY 2 cut(s) 39, 263
AcuI CTGAAG 1 cut(s) 629
AcvI CACGTG 1 cut(s) 689
AcyI GRCGYC 1 cut(s) 330
AfaI GTAC 4 cut(s) 7, 693, 1642, 1679
AfiI CCNNNNNNNGG 6 cut(s) 121, 439, 928, 932, 943, 1457
AflII CTTAAG 1 cut(s) 1330
AflIII ACRYGT 3 cut(s) 349, 392, 688
AgsI TTSAA 9 cut(s) 485, 533, 682, 1010, 1034, 1046, 1226, 1315, 1421
AjnI CCWGG 4 cut(s) 210, 718, 936, 1002
AloI GAACNNNNNNTCC 2 cut(s) 1566, 1598
Alw21I GWGCWC 2 cut(s) 898, 1131
Alw26I GTCTC 2 cut(s) 500, 1003
AlwI GGATC 2 cut(s) 1624, 1726
Ama87I CYCGRG 1 cut(s) 1636
Aor13HI TCCGGA 1 cut(s) 79
AoxI GGCC 4 cut(s) 177, 875, 934, 1252
ApaI GGGCCC 1 cut(s) 879
ApeKI GCWGC 3 cut(s) 632, 1169, 1632
ApoI RAATTY 2 cut(s) 39, 263
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AspLEI GCGC 1 cut(s) 1264
AspS9I GGNCC 4 cut(s) 875, 876, 1238, 1356
AsuC2I CCSGG 3 cut(s) 344, 1637, 1638
AsuHPI GGTGA 4 cut(s) 307, 972, 1140, 1291
AsuNHI GCTAGC 2 cut(s) 172, 1052
AvaI CYCGRG 1 cut(s) 1636
AvaII GGWCC 2 cut(s) 1238, 1356
BaeGI GKGCMC 2 cut(s) 349, 879
BanI GGYRCC 2 cut(s) 155, 194
BanII GRGCYC 3 cut(s) 848, 879, 898
BbrPI CACGTG 1 cut(s) 689
BbsI GAAGAC 2 cut(s) 730, 1332
Bbv12I GWGCWC 2 cut(s) 898, 1131
BbvCI CCTCAGC 1 cut(s) 1265
BbvI GCAGC 3 cut(s) 619, 1181, 1644
BccI CCATC 7 cut(s) 320, 515, 608, 894, 1140, 1263, 1607
BceAI ACGGC 1 cut(s) 916
BciT130I CCWGG 4 cut(s) 212, 720, 938, 1004
BcnI CCSGG 3 cut(s) 344, 1637, 1638
BcoDI GTCTC 2 cut(s) 500, 1003
BfaI CTAG 3 cut(s) 173, 660, 1053
BfmI CTRYAG 1 cut(s) 633
BfrI CTTAAG 1 cut(s) 1330
BisI GCNGC 7 cut(s) 177, 223, 364, 633, 868, 1170, 1633
BlpI GCTNAGC 2 cut(s) 367, 863
BlsI GCNGC 7 cut(s) 178, 224, 365, 634, 869, 1171, 1634
Bme1390I CCNGG 7 cut(s) 212, 344, 720, 938, 1004, 1637, 1638
Bme18I GGWCC 2 cut(s) 1238, 1356
BmeT110I CYCGRG 1 cut(s) 1636
BmgT120I GGNCC 4 cut(s) 875, 876, 1238, 1356
BmiI GGNNCC 7 cut(s) 157, 196, 431, 845, 876, 877, 1239
BmrFI CCNGG 7 cut(s) 212, 344, 720, 938, 1004, 1637, 1638
BmsI GCATC 7 cut(s) 598, 751, 849, 912, 1029, 1273, 1656
BmtI GCTAGC 2 cut(s) 176, 1056
BpiI GAAGAC 2 cut(s) 730, 1332
Bpu10I CCTNAGC 2 cut(s) 1125, 1265
Bpu1102I GCTNAGC 2 cut(s) 367, 863
BpuEI CTTGAG 2 cut(s) 1135, 1635
BpuMI CCSGG 3 cut(s) 344, 1637, 1638
Bsa29I ATCGAT 1 cut(s) 1365
BsaAI YACGTR 1 cut(s) 689
BsaBI GATNNNNATC 1 cut(s) 1287
BsaHI GRCGYC 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 1003
BsaJI CCNNGG 6 cut(s) 321, 927, 937, 1002, 1636, 1714
BsaWI WCCGGW 2 cut(s) 79, 487
Bsc4I CCNNNNNNNGG 6 cut(s) 121, 439, 928, 932, 943, 1457
Bse118I RCCGGY 2 cut(s) 830, 1414
Bse1I ACTGG 2 cut(s) 989, 1451
Bse8I GATNNNNATC 1 cut(s) 1287
BseAI TCCGGA 1 cut(s) 79
BseBI CCWGG 4 cut(s) 212, 720, 938, 1004
BseCI ATCGAT 1 cut(s) 1365
BseDI CCNNGG 6 cut(s) 321, 927, 937, 1002, 1636, 1714
BseGI GGATG 4 cut(s) 296, 331, 339, 1132
BseJI GATNNNNATC 1 cut(s) 1287
BseLI CCNNNNNNNGG 6 cut(s) 121, 439, 928, 932, 943, 1457
BseMII CTCAG 5 cut(s) 381, 854, 1116, 1219, 1256
BseNI ACTGG 2 cut(s) 989, 1451
BseSI GKGCMC 2 cut(s) 349, 879
BseXI GCAGC 3 cut(s) 619, 1181, 1644
BseYI CCCAGC 5 cut(s) 115, 301, 306, 355, 380
Bsh1236I CGCG 2 cut(s) 351, 394
BshFI GGCC 4 cut(s) 179, 877, 936, 1254
BshNI GGYRCC 2 cut(s) 155, 194
BshVI ATCGAT 1 cut(s) 1365
BsiHKAI GWGCWC 2 cut(s) 898, 1131
BsiHKCI CYCGRG 1 cut(s) 1636
BsiSI CCGG 7 cut(s) 80, 343, 488, 831, 1212, 1415, 1637
BslFI GGGAC 2 cut(s) 595, 1698
BslI CCNNNNNNNGG 6 cut(s) 121, 439, 928, 932, 943, 1457
BsmAI GTCTC 2 cut(s) 500, 1003
BsmFI GGGAC 2 cut(s) 595, 1698
BsmI GAATGC 2 cut(s) 382, 688
BsnI GGCC 4 cut(s) 179, 877, 936, 1254
Bso31I GGTCTC 1 cut(s) 1003
BsoBI CYCGRG 1 cut(s) 1636
Bsp120I GGGCCC 1 cut(s) 875
Bsp1286I GDGCHC 5 cut(s) 349, 848, 879, 898, 1131
Bsp13I TCCGGA 1 cut(s) 79
Bsp1407I TGTACA 1 cut(s) 691
Bsp143I GATC 3 cut(s) 666, 1616, 1718
Bsp1720I GCTNAGC 2 cut(s) 367, 863
Bsp19I CCATGG 1 cut(s) 927
BspACI CCGC 9 cut(s) 159, 170, 176, 222, 363, 867, 879, 947, 1056
BspANI GGCC 4 cut(s) 179, 877, 936, 1254
BspCNI CTCAG 5 cut(s) 380, 855, 1117, 1218, 1257
BspDI ATCGAT 1 cut(s) 1365
BspEI TCCGGA 1 cut(s) 79
BspFNI CGCG 2 cut(s) 351, 394
BspLI GGNNCC 7 cut(s) 157, 196, 431, 845, 876, 877, 1239
BspMAI CTGCAG 1 cut(s) 637
BspOI GCTAGC 2 cut(s) 176, 1056
BspPI GGATC 2 cut(s) 1624, 1726
BspT107I GGYRCC 2 cut(s) 155, 194
BspTI CTTAAG 1 cut(s) 1330
BspTNI GGTCTC 1 cut(s) 1003
BsrBI CCGCTC 1 cut(s) 867
BsrFI RCCGGY 2 cut(s) 830, 1414
BsrGI TGTACA 1 cut(s) 691
BsrI ACTGG 2 cut(s) 989, 1451
BssAI RCCGGY 2 cut(s) 830, 1414
BssECI CCNNGG 6 cut(s) 321, 927, 937, 1002, 1636, 1714
BssMI GATC 3 cut(s) 666, 1616, 1718
BssNI GRCGYC 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 927
Bst2UI CCWGG 4 cut(s) 212, 720, 938, 1004
Bst4CI ACNGT 6 cut(s) 194, 202, 770, 1180, 1516, 1660
Bst6I CTCTTC 1 cut(s) 1605
BstACI GRCGYC 1 cut(s) 330
BstAFI CTTAAG 1 cut(s) 1330
BstAPI GCANNNNNTGC 1 cut(s) 377
BstAUI TGTACA 1 cut(s) 691
BstBAI YACGTR 1 cut(s) 689
BstC8I GCNNGC 9 cut(s) 174, 227, 349, 353, 396, 400, 879, 1054, 1473
BstDEI CTNAG 6 cut(s) 367, 728, 863, 1125, 1205, 1265
BstDSI CCRYGG 2 cut(s) 927, 1714
BstF5I GGATG 4 cut(s) 296, 331, 339, 1132
BstFNI CGCG 2 cut(s) 351, 394
BstHHI GCGC 1 cut(s) 1264
BstKTI GATC 3 cut(s) 669, 1619, 1721
BstMAI GTCTC 2 cut(s) 500, 1003
BstMBI GATC 3 cut(s) 666, 1616, 1718
BstMWI GCNNNNNNNGC 4 cut(s) 110, 377, 1169, 1347
BstNI CCWGG 4 cut(s) 212, 720, 938, 1004
BstNSI RCATGY 2 cut(s) 1414, 1475
BstSCI CCNGG 7 cut(s) 210, 342, 718, 936, 1002, 1635, 1636
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 2 cut(s) 349, 879
BstUI CGCG 2 cut(s) 351, 394
BstV1I GCAGC 3 cut(s) 619, 1181, 1644
BstV2I GAAGAC 2 cut(s) 730, 1332
BstXI CCANNNNNNTGG 2 cut(s) 280, 1078
Bsu15I ATCGAT 1 cut(s) 1365
BsuRI GGCC 4 cut(s) 179, 877, 936, 1254
BsuTUI ATCGAT 1 cut(s) 1365
BtgI CCRYGG 2 cut(s) 927, 1714
BtsCI GGATG 4 cut(s) 296, 331, 339, 1132
BtsI GCAGTG 3 cut(s) 126, 378, 506
BtsIMutI CAGTG 4 cut(s) 126, 378, 506, 1512
Cac8I GCNNGC 9 cut(s) 174, 227, 349, 353, 396, 400, 879, 1054, 1473
CfoI GCGC 1 cut(s) 1264
Cfr10I RCCGGY 2 cut(s) 830, 1414
Cfr13I GGNCC 4 cut(s) 875, 876, 1238, 1356
Cfr9I CCCGGG 1 cut(s) 1636
ClaI ATCGAT 1 cut(s) 1365
CseI GACGC 1 cut(s) 319
Csp6I GTAC 4 cut(s) 6, 692, 1641, 1678
CspCI CAANNNNNGTGG 1 cut(s) 33
CviAII CATG 9 cut(s) 928, 1159, 1234, 1351, 1411, 1472, 1538, 1681, 1700
CviQI GTAC 4 cut(s) 6, 692, 1641, 1678
DdeI CTNAG 6 cut(s) 367, 728, 863, 1125, 1205, 1265
DpnI GATC 3 cut(s) 668, 1618, 1720
DpnII GATC 3 cut(s) 666, 1616, 1718
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 2 cut(s) 177, 934
Eam1104I CTCTTC 1 cut(s) 1605
EarI CTCTTC 1 cut(s) 1605
EciI GGCGGA 1 cut(s) 962
Ecl136II GAGCTC 1 cut(s) 896
Eco130I CCWWGG 1 cut(s) 927
Eco24I GRGCYC 3 cut(s) 848, 879, 898
Eco31I GGTCTC 1 cut(s) 1003
Eco47I GGWCC 2 cut(s) 1238, 1356
Eco53kI GAGCTC 1 cut(s) 896
Eco57I CTGAAG 1 cut(s) 629
Eco72I CACGTG 1 cut(s) 689
Eco88I CYCGRG 1 cut(s) 1636
EcoICRI GAGCTC 1 cut(s) 896
EcoO109I RGGNCCY 1 cut(s) 875
EcoRI GAATTC 1 cut(s) 39
EcoRII CCWGG 4 cut(s) 210, 718, 936, 1002
EcoT14I CCWWGG 1 cut(s) 927
EcoT22I ATGCAT 1 cut(s) 787
EcoT38I GRGCYC 3 cut(s) 848, 879, 898
ErhI CCWWGG 1 cut(s) 927
FaeI CATG 9 cut(s) 931, 1162, 1237, 1354, 1414, 1475, 1541, 1684, 1703
FalI AAGNNNNNCTT 2 cut(s) 1097, 1129
FaqI GGGAC 2 cut(s) 595, 1698
FatI CATG 9 cut(s) 927, 1158, 1233, 1350, 1410, 1471, 1537, 1680, 1699
FauI CCCGC 1 cut(s) 886
FauNDI CATATG 1 cut(s) 1526
Fnu4HI GCNGC 7 cut(s) 177, 223, 364, 633, 868, 1170, 1633
FokI GGATG 4 cut(s) 283, 326, 338, 1119
FriOI GRGCYC 3 cut(s) 848, 879, 898
Fsp4HI GCNGC 7 cut(s) 177, 223, 364, 633, 868, 1170, 1633
FspBI CTAG 3 cut(s) 173, 660, 1053
GlaI GCGC 1 cut(s) 1263
GluI GCNGC 7 cut(s) 177, 223, 364, 633, 868, 1170, 1633
GsaI CCCAGC 5 cut(s) 119, 305, 310, 359, 384
HaeIII GGCC 4 cut(s) 179, 877, 936, 1254
HapII CCGG 7 cut(s) 80, 343, 488, 831, 1212, 1415, 1637
HgaI GACGC 1 cut(s) 319
HhaI GCGC 1 cut(s) 1264
Hin1I GRCGYC 1 cut(s) 330
Hin1II CATG 9 cut(s) 931, 1162, 1237, 1354, 1414, 1475, 1541, 1684, 1703
Hin6I GCGC 1 cut(s) 1262
HinP1I GCGC 1 cut(s) 1262
HincII GTYRAC 2 cut(s) 1443, 1456
HindII GTYRAC 2 cut(s) 1443, 1456
HindIII AAGCTT 1 cut(s) 738
HinfI GANTC 6 cut(s) 540, 961, 1117, 1215, 1595, 1693
HpaI GTTAAC 1 cut(s) 1456
HpaII CCGG 7 cut(s) 80, 343, 488, 831, 1212, 1415, 1637
HphI GGTGA 4 cut(s) 307, 972, 1140, 1291
Hpy188I TCNGA 8 cut(s) 70, 243, 322, 518, 893, 1208, 1381, 1673
Hpy188III TCNNGA 2 cut(s) 80, 1114
Hpy99I CGWCG 1 cut(s) 1624
HpyAV CCTTC 6 cut(s) 892, 1040, 1076, 1455, 1551, 1668
HpyCH4III ACNGT 6 cut(s) 194, 202, 770, 1180, 1516, 1660
HpyCH4IV ACGT 2 cut(s) 688, 1622
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 377, 1169, 1347
HpyF3I CTNAG 6 cut(s) 367, 728, 863, 1125, 1205, 1265
HpySE526I ACGT 2 cut(s) 688, 1622
Hsp92I GRCGYC 1 cut(s) 330
Hsp92II CATG 9 cut(s) 931, 1162, 1237, 1354, 1414, 1475, 1541, 1684, 1703
HspAI GCGC 1 cut(s) 1262
Kpn2I TCCGGA 1 cut(s) 79
KspAI GTTAAC 1 cut(s) 1456
Kzo9I GATC 3 cut(s) 666, 1616, 1718
LmnI GCTCC 4 cut(s) 20, 82, 843, 1049
Lsp1109I GCAGC 3 cut(s) 619, 1181, 1644
LweI GCATC 7 cut(s) 598, 751, 849, 912, 1029, 1273, 1656
MaeI CTAG 3 cut(s) 173, 660, 1053
MaeII ACGT 2 cut(s) 688, 1622
MaeIII GTNAC 2 cut(s) 1297, 1400
MalI GATC 3 cut(s) 668, 1618, 1720
MbiI CCGCTC 1 cut(s) 867
MboI GATC 3 cut(s) 666, 1616, 1718
MboII GAAGA 6 cut(s) 20, 735, 820, 956, 1337, 1592
MhlI GDGCHC 5 cut(s) 349, 848, 879, 898, 1131
MluCI AATT 6 cut(s) 39, 263, 816, 1188, 1499, 1732
MluI ACGCGT 2 cut(s) 349, 392
MlyI GAGTC 3 cut(s) 549, 1126, 1224
MmeI TCCRAC 2 cut(s) 407, 1534
Mph1103I ATGCAT 1 cut(s) 787
MroI TCCGGA 1 cut(s) 79
MseI TTAA 5 cut(s) 1104, 1305, 1331, 1431, 1455
MslI CAYNNNNRTG 4 cut(s) 576, 908, 1517, 1536
MspA1I CMGCKG 2 cut(s) 632, 1172
MspCI CTTAAG 1 cut(s) 1330
MspI CCGG 7 cut(s) 80, 343, 488, 831, 1212, 1415, 1637
MspR9I CCNGG 7 cut(s) 212, 344, 720, 938, 1004, 1637, 1638
Mva1269I GAATGC 2 cut(s) 382, 688
MvaI CCWGG 4 cut(s) 212, 720, 938, 1004
MvnI CGCG 2 cut(s) 351, 394
MwoI GCNNNNNNNGC 4 cut(s) 110, 377, 1169, 1347
NciI CCSGG 3 cut(s) 344, 1637, 1638
NcoI CCATGG 1 cut(s) 927
NdeI CATATG 1 cut(s) 1526
NdeII GATC 3 cut(s) 666, 1616, 1718
NheI GCTAGC 2 cut(s) 172, 1052
NlaIII CATG 9 cut(s) 931, 1162, 1237, 1354, 1414, 1475, 1541, 1684, 1703
NlaIV GGNNCC 7 cut(s) 157, 196, 431, 845, 876, 877, 1239
NmuCI GTSAC 1 cut(s) 1297
NsiI ATGCAT 1 cut(s) 787
NspI RCATGY 2 cut(s) 1414, 1475
PaeI GCATGC 1 cut(s) 1475
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 2 cut(s) 382, 688
PfeI GAWTC 3 cut(s) 961, 1595, 1693
PflMI CCANNNNNTGG 3 cut(s) 439, 928, 943
PfoI TCCNGGA 2 cut(s) 210, 718
PkrI GCNGC 7 cut(s) 178, 224, 365, 634, 869, 1171, 1634
PleI GAGTC 3 cut(s) 548, 1125, 1223
PmaCI CACGTG 1 cut(s) 689
PmlI CACGTG 1 cut(s) 689
PpsI GAGTC 3 cut(s) 548, 1125, 1223
Ppu21I YACGTR 1 cut(s) 689
Psp124BI GAGCTC 1 cut(s) 898
Psp6I CCWGG 4 cut(s) 210, 718, 936, 1002
PspCI CACGTG 1 cut(s) 689
PspFI CCCAGC 5 cut(s) 115, 301, 306, 355, 380
PspGI CCWGG 4 cut(s) 210, 718, 936, 1002
PspN4I GGNNCC 7 cut(s) 157, 196, 431, 845, 876, 877, 1239
PspOMI GGGCCC 1 cut(s) 875
PspPI GGNCC 4 cut(s) 875, 876, 1238, 1356
PstI CTGCAG 1 cut(s) 637
PvuII CAGCTG 2 cut(s) 632, 1172
RsaI GTAC 4 cut(s) 7, 693, 1642, 1679
RsaNI GTAC 4 cut(s) 6, 692, 1641, 1678
RseI CAYNNNNRTG 4 cut(s) 576, 908, 1517, 1536
SacI GAGCTC 1 cut(s) 898
SaqAI TTAA 5 cut(s) 1104, 1305, 1331, 1431, 1455
SatI GCNGC 7 cut(s) 177, 223, 364, 633, 868, 1170, 1633
Sau3AI GATC 3 cut(s) 666, 1616, 1718
Sau96I GGNCC 4 cut(s) 875, 876, 1238, 1356
SchI GAGTC 3 cut(s) 549, 1126, 1224
ScrFI CCNGG 7 cut(s) 212, 344, 720, 938, 1004, 1637, 1638
SduI GDGCHC 5 cut(s) 349, 848, 879, 898, 1131
SfaNI GCATC 7 cut(s) 598, 751, 849, 912, 1029, 1273, 1656
SfcI CTRYAG 1 cut(s) 633
SinI GGWCC 2 cut(s) 1238, 1356
SmaI CCCGGG 1 cut(s) 1638
SmiMI CAYNNNNRTG 4 cut(s) 576, 908, 1517, 1536
SmlI CTYRAG 3 cut(s) 1114, 1330, 1650
SmoI CTYRAG 3 cut(s) 1114, 1330, 1650
SphI GCATGC 1 cut(s) 1475
Sse9I AATT 6 cut(s) 39, 263, 816, 1188, 1499, 1732
SsiI CCGC 9 cut(s) 159, 170, 176, 222, 363, 867, 879, 947, 1056
SspMI CTAG 3 cut(s) 173, 660, 1053
SstI GAGCTC 1 cut(s) 898
StyD4I CCNGG 7 cut(s) 210, 342, 718, 936, 1002, 1635, 1636
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 6 cut(s) 194, 202, 770, 1180, 1516, 1660
TaiI ACGT 2 cut(s) 691, 1625
TaqI TCGA 3 cut(s) 1365, 1619, 1721
TasI AATT 6 cut(s) 39, 263, 816, 1188, 1499, 1732
TatI WGTACW 1 cut(s) 691
TauI GCSGC 4 cut(s) 179, 225, 366, 870
TfiI GAWTC 3 cut(s) 961, 1595, 1693
Tru1I TTAA 5 cut(s) 1104, 1305, 1331, 1431, 1455
Tru9I TTAA 5 cut(s) 1104, 1305, 1331, 1431, 1455
TscAI CASTG 4 cut(s) 126, 378, 506, 1519
TseFI GTSAC 1 cut(s) 1297
TseI GCWGC 3 cut(s) 632, 1169, 1632
Tsp45I GTSAC 1 cut(s) 1297
TspDTI ATGAA 4 cut(s) 471, 812, 828, 974
TspGWI ACGGA 4 cut(s) 1200, 1388, 1473, 1703
TspMI CCCGGG 1 cut(s) 1636
TspRI CASTG 4 cut(s) 126, 378, 506, 1519
Van91I CCANNNNNTGG 3 cut(s) 439, 928, 943
Vha464I CTTAAG 1 cut(s) 1330
VpaK11BI GGWCC 2 cut(s) 1238, 1356
XapI RAATTY 2 cut(s) 39, 263
XceI RCATGY 2 cut(s) 1414, 1475
XmaI CCCGGG 1 cut(s) 1636
XspI CTAG 3 cut(s) 173, 660, 1053
Zsp2I ATGCAT 1 cut(s) 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.