Rorug01G0396200

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0010788
Physical Location & Seq
Forward (+)
50580672 .. 50582741
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0396200.1

Sequence Viewer

Length: 2070 bp
ATGGATGTGGCTCTAATTCCTCCAATGTTGGTTTCTTCAGGCTTTTTGCCTACTGGGTCATTCTTAGAGTCATTGATTCACATATGCAATGAGATTTGCTCCATGGAAAAGCTTCCTTTTCTTCAAGTCAGGAACATCTCCACCATGATAAGGAGGATTAAGCTTCTTTCTTCTTTGTTCGAAGAGATTCAAGAAACTAACGGCCCTCTCCCTCCATCTTCAATCCTGTGCCTCACCGAGCTCTTCTCCGTGATTCGAAGAATCAAGTTTTTGATTCAAGAGTGTAAGGACTGCAGCTTTTTGTGGGGACTTATGCAGATGGAGCTGGTTTCAAATCAGTTCTACGTGTTGGTCAAGGAGTTAGGGAGAGCACTTGATATTTTGCCTGTGAGTTTGCTCAATATTACGGCAGATATTAGAGAGCAGATTGAGCTTCTTCACCGGCAAGCGAAAAGGGCAGAGTTGTTTATTGACCTTAAAGAGGTGAAGAGAAGAGAAGAGCTTTTGGTATTGATGGGGAGCAACAATGAGAGGAACAAGAAGAACAAAGGGTTTGTAGACTTGGGCAAGGTGAAGGATATCTTGAGCAGCATTGGCTTGAGAAGCTCAATGGATTACGAGGAAGAGATTTCAAAGCTAGAGGCAGAAGCTCAAAAGCAAGCAGGGAGTGGCGGACTAATTGTGGTATGCAATATAAACAACCTCATCTCCCTTGTTTCATATTGCAAATCCATGATTTTCGTCTATGATGATTTCGAAACAACCAACAAGGAAGACTTCAAGCTGCTAGGTTCTACACCCTCAAATAGATATTATGATCACTCCTCATCTTCTCAATCCATAGTTGCCAACATCCCAGATGAATTTCGATGCCCGATTTCACTAGACTTGATGAGAGATCCTGTGATAGTAGCATCGGGGCATACATATGATCGAAATTCCATTGCTCAGTGGATCAATTCAGGGCACCAAACATGCCCCAAAAGTGGACAGAAGCTTATTCACATGGCCCTTATACCTAACTATGCACTGAAGAGTCTATTGCATCAATGGTGTGAGGAAAACAATGTTCCAGAAGTTGGATCATCACCATCATCTTCTTCAGATTTGGAGAGGAGCAGGAGCAAAAGAGATTCTTGTGAAAATGCTGTGGATCACATTTCTGTTGTAAAAGCTGCAGTTGATGCTGTGAAGTTAACAGCTGAGTTTCTGGTGGGAAAACTAGCAACAGGTTCACCAGATATCCAAAGACAAGCAGCATACGAGCTTCGGTTACTTGCTAAAACAGGCATGGATAACAGAAGAATAATAGCTGAAGCAGGAGCTATTCCTTTCTTAGTGACATTGCTGAGATCACATGATCCAAGAATTCAGGAAAATGCTGTCACCGCACTACTCAACCTTTCCATCTTCAACAACAACAAAATTTTGATAATGGCTGCAGGAGCAATTGATTACATAATCGATGTGTTGGAATCAGGGAACACAATGGAAGCAAGAGAGAATGCAGCAGCAGCAATTTTCAGCTTGTCCATTATTGATGACTGCAAGGTGACAATTGGAAAAAGACCAAGAGCCATTCCGGCATTGGTGGGGCTTTTGAAAGAAGGAACTCCGGCTGGTAAAAAGGACGCAGCCATTGCTCTCTTCAACCTTGCAGTCTACAATGCTAACAAGGTCAGTCTGGTGGTTGCTGGGGCGGGTCCCCTGCTCATTGATCTGTTGATGGATGACAAGGCGGGGATAACAGATGATGCCTTGGCAGTTCTTGCTCAACTTTTGGGATGCTCTGAAGGGTTGGAGGAGATTGGGAAGAGTAGGGTTCTAGTGCCAATTCTCATTGATCTTCTGAGATTTGGTTCTCCAAAAGGGAAGGAGAATTCAATAACTCTTCTGTTGGGGTTGTGCAAAGATGGAGGAGAGGAGGTTGCAAGACGACTATTGATGAACCCGCGGAGTATCCCTTCTCTTCAAAGCTTGGTTGCTGATGGATCCTTGAAAGCTCGAAGAAAAGCCGATGCACTGCTTAGATTACTGAACAGACGCTGCTTTCAATCTCAGAATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

689

Amino Acids

75.88

Weight (kDa)

6.56

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCAfunc PF19584 43 - 151 1.4e-06 MCAfunc domain
PUB10_N PF25368 46 - 153 2.8e-15 PUB10-like, N-terminal domain
U-box PF04564 285 - 356 4.1e-22 U-box domain
zf-Nse PF11789 288 - 330 4.8e-06 Zinc-finger of the MIZ type in Nse subunit
zf-RING_UBOX PF13445 291 - 327 5.2e-06 RING-type zinc-finger
ARM_PUB PF25598 417 - 683 2.7e-22 Plant U-box protein ARM repeat region
Arm PF00514 432 - 469 1.6e-10 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 432 - 617 1.9e-08 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010788)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56060 AT5G51930 AT5G51930 AT5G51950 AT5G51950 AT5G51950
fragaria_vesca FvH4_7g28850
malus_domestica MD01G1194300.v1.1
prunus_persica Prupe.2G288400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0376521
rosa_laevigata RLG00000026604
rosa_multiflora Rmu_sc0001308.1_g000024 Rmu_sc0016049.1_g000002
rosa_roxburghii Rroxscaffold_4G00282040
rosa_rugosa Rorug01G0396200
rosa_samantha Rh1AG408000 Rh1BG372000 Rh1CG385800 Rh1DG403000
rosa_wichuraiana Rw1G036150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 966
AccB7I CCANNNNNTGG 1 cut(s) 1079
AccI GTMKAC 2 cut(s) 558, 1662
AccII CGCG 1 cut(s) 1954
AciI CCGC 6 cut(s) 672, 1389, 1700, 1739, 1952, 1954
AclWI GGATC 7 cut(s) 893, 962, 1090, 1161, 1355, 1986, 1999
AcsI RAATTY 5 cut(s) 863, 937, 1368, 1425, 1879
AcuI CTGAAG 5 cut(s) 21, 1052, 1086, 1335, 1812
AfiI CCNNNNNNNGG 4 cut(s) 150, 481, 986, 1079
AflIII ACRYGT 1 cut(s) 345
AjuI GAANNNNNNNTTGG 2 cut(s) 16, 48
Alw21I GWGCWC 2 cut(s) 243, 373
AlwI GGATC 7 cut(s) 893, 962, 1090, 1161, 1355, 1986, 1999
AlwNI CAGNNNCTG 1 cut(s) 2046
AoxI GGCC 2 cut(s) 202, 1008
ApoI RAATTY 5 cut(s) 863, 937, 1368, 1425, 1879
Asp700I GAANNNNTTC 2 cut(s) 111, 186
AspS9I GGNCC 3 cut(s) 203, 1009, 1703
AsuHPI GGTGA 8 cut(s) 226, 431, 496, 583, 1080, 1227, 1378, 1564
AsuII TTCGAA 3 cut(s) 180, 256, 756
AvaII GGWCC 1 cut(s) 1703
BaeGI GKGCMC 1 cut(s) 969
BamHI GGATCC 1 cut(s) 1991
BanI GGYRCC 1 cut(s) 966
BanII GRGCYC 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 780
Bbv12I GWGCWC 2 cut(s) 243, 373
BccI CCATC 8 cut(s) 223, 313, 508, 1099, 1415, 1720, 1907, 1982
BceAI ACGGC 2 cut(s) 217, 423
BciVI GTATCC 1 cut(s) 1970
BclI TGATCA 1 cut(s) 817
BfaI CTAG 5 cut(s) 638, 788, 884, 1223, 1826
BfmI CTRYAG 3 cut(s) 292, 1176, 1440
BfuI GTATCC 1 cut(s) 1970
BglI GCCNNNNNGGC 1 cut(s) 1583
Bme18I GGWCC 1 cut(s) 1703
BmgT120I GGNCC 3 cut(s) 203, 1009, 1703
BmiI GGNNCC 4 cut(s) 968, 1704, 1705, 1993
BmrI ACTGGG 1 cut(s) 63
BmsI GCATC 7 cut(s) 860, 923, 1054, 1174, 1744, 1775, 2008
BmuI ACTGGG 1 cut(s) 63
BpiI GAAGAC 1 cut(s) 780
BplI GAGNNNNNCTC 4 cut(s) 83, 115, 230, 262
Bpu14I TTCGAA 3 cut(s) 180, 256, 756
BpuEI CTTGAG 2 cut(s) 604, 619
Bsa29I ATCGAT 1 cut(s) 1464
BsaAI YACGTR 1 cut(s) 346
BsaJI CCNNGG 3 cut(s) 102, 1758, 1952
BsaXI ACNNNNNCTCC 4 cut(s) 692, 722, 1911, 1941
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 481, 986, 1079
Bse118I RCCGGY 1 cut(s) 441
Bse1I ACTGG 1 cut(s) 58
Bse3DI GCAATG 4 cut(s) 94, 942, 1343, 1638
BseCI ATCGAT 1 cut(s) 1464
BseDI CCNNGG 3 cut(s) 102, 1758, 1952
BseGI GGATG 4 cut(s) 10, 852, 1735, 1790
BseLI CCNNNNNNNGG 4 cut(s) 150, 481, 986, 1079
BseMI GCAATG 4 cut(s) 94, 942, 1343, 1638
BseMII CTCAG 4 cut(s) 962, 1194, 1340, 1841
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 5 cut(s) 814, 1129, 1817, 1932, 1937
BseSI GKGCMC 1 cut(s) 969
BseYI CCCAGC 1 cut(s) 1694
Bsh1236I CGCG 1 cut(s) 1954
BshFI GGCC 2 cut(s) 204, 1010
BshNI GGYRCC 1 cut(s) 966
BshVI ATCGAT 1 cut(s) 1464
BsiHKAI GWGCWC 2 cut(s) 243, 373
BsiSI CCGG 3 cut(s) 442, 1583, 1616
BslFI GGGAC 2 cut(s) 321, 1689
BslI CCNNNNNNNGG 4 cut(s) 150, 481, 986, 1079
BsmFI GGGAC 2 cut(s) 321, 1689
BsmI GAATGC 1 cut(s) 1510
BsnI GGCC 2 cut(s) 204, 1010
Bsp119I TTCGAA 3 cut(s) 180, 256, 756
Bsp1286I GDGCHC 3 cut(s) 243, 373, 969
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 6 cut(s) 672, 1389, 1700, 1739, 1952, 1954
BspANI GGCC 2 cut(s) 204, 1010
BspCNI CTCAG 4 cut(s) 961, 1195, 1341, 1842
BspDI ATCGAT 1 cut(s) 1464
BspFNI CGCG 1 cut(s) 1954
BspLI GGNNCC 4 cut(s) 968, 1704, 1705, 1993
BspMAI CTGCAG 3 cut(s) 296, 1180, 1444
BspPI GGATC 7 cut(s) 893, 962, 1090, 1161, 1355, 1986, 1999
BspQI GCTCTTC 2 cut(s) 248, 492
BspT104I TTCGAA 3 cut(s) 180, 256, 756
BspT107I GGYRCC 1 cut(s) 966
BsrDI GCAATG 4 cut(s) 94, 942, 1343, 1638
BsrFI RCCGGY 1 cut(s) 441
BsrI ACTGG 1 cut(s) 58
BssAI RCCGGY 1 cut(s) 441
BssECI CCNNGG 3 cut(s) 102, 1758, 1952
BssT1I CCWWGG 2 cut(s) 102, 1758
BstAPI GCANNNNNTGC 3 cut(s) 1184, 1640, 1769
BstBAI YACGTR 1 cut(s) 346
BstBI TTCGAA 3 cut(s) 180, 256, 756
BstC8I GCNNGC 2 cut(s) 447, 660
BstDEI CTNAG 8 cut(s) 64, 948, 1203, 1336, 1349, 1850, 2027, 2058
BstDSI CCRYGG 2 cut(s) 102, 1952
BstENI CCTNNNNNAGG 1 cut(s) 479
BstF5I GGATG 4 cut(s) 10, 852, 1735, 1790
BstFNI CGCG 1 cut(s) 1954
BstNSI RCATGY 1 cut(s) 978
BstSFI CTRYAG 3 cut(s) 292, 1176, 1440
BstSLI GKGCMC 1 cut(s) 969
BstUI CGCG 1 cut(s) 1954
BstV2I GAAGAC 1 cut(s) 780
BstX2I RGATCY 2 cut(s) 898, 1991
BstYI RGATCY 2 cut(s) 898, 1991
Bsu15I ATCGAT 1 cut(s) 1464
BsuI GTATCC 1 cut(s) 1970
BsuRI GGCC 2 cut(s) 204, 1010
BsuTUI ATCGAT 1 cut(s) 1464
BtgI CCRYGG 2 cut(s) 102, 1952
BtsCI GGATG 4 cut(s) 10, 852, 1735, 1790
BtsI GCAGTG 1 cut(s) 2021
BtsIMutI CAGTG 3 cut(s) 956, 1028, 2021
Cac8I GCNNGC 2 cut(s) 447, 660
CaiI CAGNNNCTG 1 cut(s) 2046
Cfr10I RCCGGY 1 cut(s) 441
Cfr13I GGNCC 3 cut(s) 203, 1009, 1703
Cfr42I CCGCGG 1 cut(s) 1955
ClaI ATCGAT 1 cut(s) 1464
CseI GACGC 2 cut(s) 1640, 2052
CviAII CATG 7 cut(s) 103, 145, 733, 975, 1006, 1291, 1358
DdeI CTNAG 8 cut(s) 64, 948, 1203, 1336, 1349, 1850, 2027, 2058
EciI GGCGGA 1 cut(s) 687
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 102, 1758
Eco24I GRGCYC 1 cut(s) 243
Eco32I GATATC 2 cut(s) 580, 1243
Eco47I GGWCC 1 cut(s) 1703
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 5 cut(s) 21, 1052, 1086, 1335, 1812
EcoICRI GAGCTC 1 cut(s) 241
EcoNI CCTNNNNNAGG 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 1703
EcoRI GAATTC 2 cut(s) 1368, 1879
EcoRV GATATC 2 cut(s) 580, 1243
EcoT14I CCWWGG 2 cut(s) 102, 1758
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 2 cut(s) 102, 1758
FaeI CATG 7 cut(s) 106, 148, 736, 978, 1009, 1294, 1361
FalI AAGNNNNNCTT 6 cut(s) 566, 598, 761, 793, 1120, 1152
FaqI GGGAC 2 cut(s) 321, 1689
FatI CATG 7 cut(s) 102, 144, 732, 974, 1005, 1290, 1357
FauI CCCGC 3 cut(s) 1693, 1732, 1959
FauNDI CATATG 2 cut(s) 83, 928
FbaI TGATCA 1 cut(s) 817
FblI GTMKAC 2 cut(s) 558, 1662
FokI GGATG 4 cut(s) 17, 839, 1742, 1797
FriOI GRGCYC 1 cut(s) 243
FspBI CTAG 5 cut(s) 638, 788, 884, 1223, 1826
GsaI CCCAGC 1 cut(s) 1698
HaeIII GGCC 2 cut(s) 204, 1010
HapII CCGG 3 cut(s) 442, 1583, 1616
HgaI GACGC 2 cut(s) 1640, 2052
Hin1II CATG 7 cut(s) 106, 148, 736, 978, 1009, 1294, 1361
HincII GTYRAC 1 cut(s) 1197
HindII GTYRAC 1 cut(s) 1197
HindIII AAGCTT 4 cut(s) 110, 161, 995, 1975
HpaI GTTAAC 1 cut(s) 1197
HpaII CCGG 3 cut(s) 442, 1583, 1616
HphI GGTGA 8 cut(s) 226, 431, 496, 583, 1080, 1227, 1378, 1564
Hpy166II GTNNAC 5 cut(s) 559, 989, 1197, 1235, 1663
Hpy188I TCNGA 4 cut(s) 1105, 1792, 1851, 2061
Hpy188III TCNNGA 6 cut(s) 130, 191, 278, 583, 1073, 1373
Hpy8I GTNNAC 5 cut(s) 559, 989, 1197, 1235, 1663
HpyAV CCTTC 5 cut(s) 568, 1601, 1787, 1867, 1974
HpyCH4IV ACGT 1 cut(s) 345
HpyF3I CTNAG 8 cut(s) 64, 948, 1203, 1336, 1349, 1850, 2027, 2058
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 7 cut(s) 106, 148, 736, 978, 1009, 1294, 1361
KflI GGGWCCC 1 cut(s) 1703
Ksp22I TGATCA 1 cut(s) 817
KspAI GTTAAC 1 cut(s) 1197
KspI CCGCGG 1 cut(s) 1955
LguI GCTCTTC 2 cut(s) 248, 492
LmnI GCTCC 7 cut(s) 104, 322, 519, 1116, 1122, 1322, 1445
LweI GCATC 7 cut(s) 860, 923, 1054, 1174, 1744, 1775, 2008
MaeI CTAG 5 cut(s) 638, 788, 884, 1223, 1826
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 4 cut(s) 1272, 1339, 1384, 1552
MfeI CAATTG 2 cut(s) 1449, 1557
MflI RGATCY 2 cut(s) 898, 1991
MhlI GDGCHC 3 cut(s) 243, 373, 969
MlyI GAGTC 2 cut(s) 77, 1045
MmeI TCCRAC 3 cut(s) 1060, 1452, 1779
MroXI GAANNNNTTC 2 cut(s) 111, 186
MseI TTAA 3 cut(s) 159, 477, 1196
MslI CAYNNNNRTG 1 cut(s) 927
MspA1I CMGCKG 2 cut(s) 1202, 1954
MspI CCGG 3 cut(s) 442, 1583, 1616
MunI CAATTG 2 cut(s) 1449, 1557
Mva1269I GAATGC 1 cut(s) 1510
MvnI CGCG 1 cut(s) 1954
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 2 cut(s) 83, 928
NlaIII CATG 7 cut(s) 106, 148, 736, 978, 1009, 1294, 1361
NlaIV GGNNCC 4 cut(s) 968, 1704, 1705, 1993
NmuCI GTSAC 3 cut(s) 1339, 1384, 1552
NspI RCATGY 1 cut(s) 978
NspV TTCGAA 3 cut(s) 180, 256, 756
PciSI GCTCTTC 2 cut(s) 248, 492
PctI GAATGC 1 cut(s) 1510
PdmI GAANNNNTTC 2 cut(s) 111, 186
PfeI GAWTC 8 cut(s) 76, 187, 253, 261, 274, 1133, 1475, 2062
PflMI CCANNNNNTGG 1 cut(s) 1079
PleI GAGTC 2 cut(s) 76, 1044
PpsI GAGTC 2 cut(s) 76, 1044
Ppu21I YACGTR 1 cut(s) 346
PpuMI RGGWCCY 1 cut(s) 1703
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 1703
PspFI CCCAGC 1 cut(s) 1694
PspN4I GGNNCC 4 cut(s) 968, 1704, 1705, 1993
PspPI GGNCC 3 cut(s) 203, 1009, 1703
PspPPI RGGWCCY 1 cut(s) 1703
PstI CTGCAG 3 cut(s) 296, 1180, 1444
PstNI CAGNNNCTG 1 cut(s) 2046
PsuI RGATCY 2 cut(s) 898, 1991
PvuII CAGCTG 1 cut(s) 1202
RseI CAYNNNNRTG 1 cut(s) 927
SacI GAGCTC 1 cut(s) 243
SacII CCGCGG 1 cut(s) 1955
SapI GCTCTTC 2 cut(s) 248, 492
SaqAI TTAA 3 cut(s) 159, 477, 1196
Sau96I GGNCC 3 cut(s) 203, 1009, 1703
SchI GAGTC 2 cut(s) 77, 1045
SduI GDGCHC 3 cut(s) 243, 373, 969
SfaNI GCATC 7 cut(s) 860, 923, 1054, 1174, 1744, 1775, 2008
SfcI CTRYAG 3 cut(s) 292, 1176, 1440
Sfr303I CCGCGG 1 cut(s) 1955
SfuI TTCGAA 3 cut(s) 180, 256, 756
SgrBI CCGCGG 1 cut(s) 1955
SinI GGWCC 1 cut(s) 1703
SmiMI CAYNNNNRTG 1 cut(s) 927
SmlI CTYRAG 2 cut(s) 583, 598
SmoI CTYRAG 2 cut(s) 583, 598
SsiI CCGC 6 cut(s) 672, 1389, 1700, 1739, 1952, 1954
SspI AATATT 1 cut(s) 403
SspMI CTAG 5 cut(s) 638, 788, 884, 1223, 1826
SstI GAGCTC 1 cut(s) 243
StyI CCWWGG 2 cut(s) 102, 1758
TaiI ACGT 1 cut(s) 348
TaqI TCGA 7 cut(s) 180, 256, 756, 868, 934, 1464, 2005
TfiI GAWTC 8 cut(s) 76, 187, 253, 261, 274, 1133, 1475, 2062
Tru1I TTAA 3 cut(s) 159, 477, 1196
Tru9I TTAA 3 cut(s) 159, 477, 1196
TscAI CASTG 3 cut(s) 956, 1035, 2028
TseFI GTSAC 3 cut(s) 1339, 1384, 1552
Tsp45I GTSAC 3 cut(s) 1339, 1384, 1552
TspDTI ATGAA 3 cut(s) 708, 876, 1961
TspGWI ACGGA 1 cut(s) 238
TspRI CASTG 3 cut(s) 956, 1035, 2028
Van91I CCANNNNNTGG 1 cut(s) 1079
VpaK11BI GGWCC 1 cut(s) 1703
XagI CCTNNNNNAGG 1 cut(s) 479
XapI RAATTY 5 cut(s) 863, 937, 1368, 1425, 1879
XceI RCATGY 1 cut(s) 978
XcmI CCANNNNNNNNNTGG 1 cut(s) 1585
XmiI GTMKAC 2 cut(s) 558, 1662
XmnI GAANNNNTTC 2 cut(s) 111, 186
XspI CTAG 5 cut(s) 638, 788, 884, 1223, 1826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.