Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0016633

Physical Location & Seq

Genomic Coordinates Forward (+)

29562645 .. 29563097

Gene Structure 453 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1197500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 318 bp

ATGGCATCCTTCAAGTCTCCTATCAACTCCTTCAGCTGCTTCCAAGCTGCTACAACCCTTGTGTTGTTGATTGCCCTGAGCCTCTCAGTGCACACTCAGAAGGCTGATGCTCAGAGCAGCAGTTGCTCGGCCGCGCTGAGCAGCCTAAATGTGTGCGCACCTTTTGTGGTGCCTGGCTCCACCAACACCAATCCGAACTCTGACTGCTGTGGCGCGCTCCAAGCCGTCCATCCTGAATGCCTTTGCAACGCTGCCAGGGTCGCCGCTCGCCTCCCCGCCCAGTGCAATCTCTCCCCCATCACTTGTGACACCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005319 GO:0005548 GO:0006649 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0008150 GO:0009987 GO:0010876 GO:0015711 GO:0015748 GO:0015914 GO:0016043 GO:0033036 GO:0051179 GO:0051234 GO:0061024 GO:0071702 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LTP_2 Tryp_alpha_amyl

Protein Analysis

106

Amino Acids

10.76

Weight (kDa)

6.66

Isoelectric Point (pI)

44.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LTP_2	PF14368	24 - 87	2.7e-08	Probable lipid transfer
Tryp_alpha_amyl	PF00234	42 - 101	2.5e-07	Protease inhibitor/seed storage/LTP family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016633)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07230 AT5G52160 AT5G62080
fragaria_vesca	FvH4_7g29091
malus_domestica	MD01G1197500.v1.1
prunus_persica	Prupe.2G290900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0376811
rosa_laevigata	RLG00000026568
rosa_roxburghii	Rroxscaffold_4G00281700
rosa_samantha	Rh1AG415900 Rh1BG375300 Rh1CG389100 Rh1DG405900
rosa_wichuraiana	Rw1G036480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	157
AccB1I	GGYRCC	1 cut(s)	169
AccBSI	CCGCTC	1 cut(s)	266
AccII	CGCG	2 cut(s)	134, 215
AciI	CCGC	3 cut(s)	132, 264, 276
AcoI	YGGCCR	1 cut(s)	129
AcuI	CTGAAG	1 cut(s)	16
AgsI	TTSAA	1 cut(s)	13
AjnI	CCWGG	2 cut(s)	172, 254
AluBI	AGCT	2 cut(s)	36, 47
AluI	AGCT	2 cut(s)	36, 47
Alw21I	GWGCWC	1 cut(s)	93
Alw26I	GTCTC	1 cut(s)	21
Alw44I	GTGCAC	1 cut(s)	89
AoxI	GGCC	1 cut(s)	129
ApaLI	GTGCAC	1 cut(s)	89
ApeKI	GCWGC	5 cut(s)	36, 47, 117, 141, 251
AspLEI	GCGC	4 cut(s)	136, 158, 215, 217
BaeGI	GKGCMC	1 cut(s)	93
BanI	GGYRCC	1 cut(s)	169
Bbv12I	GWGCWC	1 cut(s)	93
BbvI	GCAGC	5 cut(s)	23, 34, 129, 153, 238
BccI	CCATC	2 cut(s)	237, 305
BceAI	ACGGC	1 cut(s)	209
BciT130I	CCWGG	2 cut(s)	174, 256
BcoDI	GTCTC	1 cut(s)	21
BisI	GCNGC	7 cut(s)	37, 48, 118, 132, 142, 252, 264
BlpI	GCTNAGC	1 cut(s)	137
BlsI	GCNGC	7 cut(s)	38, 49, 119, 133, 143, 253, 265
Bme1390I	CCNGG	2 cut(s)	174, 256
BmiI	GGNNCC	2 cut(s)	171, 178
BmrFI	CCNGG	2 cut(s)	174, 256
BmrI	ACTGGG	1 cut(s)	274
BmsI	GCATC	2 cut(s)	14, 97
BmuI	ACTGGG	1 cut(s)	274
Bpu10I	CCTNAGC	1 cut(s)	77
Bpu1102I	GCTNAGC	1 cut(s)	137
BsaJI	CCNNGG	1 cut(s)	255
Bse1I	ACTGG	1 cut(s)	280
BseBI	CCWGG	2 cut(s)	174, 256
BseDI	CCNNGG	1 cut(s)	255
BseGI	GGATG	2 cut(s)	5, 229
BseMII	CTCAG	5 cut(s)	68, 99, 110, 125, 128
BseNI	ACTGG	1 cut(s)	280
BsePI	GCGCGC	1 cut(s)	213
BseSI	GKGCMC	1 cut(s)	93
BseX3I	CGGCCG	1 cut(s)	129
BseXI	GCAGC	5 cut(s)	23, 34, 129, 153, 238
Bsh1236I	CGCG	2 cut(s)	134, 215
Bsh1285I	CGRYCG	1 cut(s)	132
BshFI	GGCC	1 cut(s)	131
BshNI	GGYRCC	1 cut(s)	169
BsiEI	CGRYCG	1 cut(s)	132
BsiHKAI	GWGCWC	1 cut(s)	93
BsmAI	GTCTC	1 cut(s)	21
BsmI	GAATGC	1 cut(s)	242
BsnI	GGCC	1 cut(s)	131
Bsp1286I	GDGCHC	1 cut(s)	93
Bsp1720I	GCTNAGC	1 cut(s)	137
BspACI	CCGC	3 cut(s)	132, 264, 276
BspANI	GGCC	1 cut(s)	131
BspCNI	CTCAG	5 cut(s)	69, 98, 109, 124, 129
BspFNI	CGCG	2 cut(s)	134, 215
BspLI	GGNNCC	2 cut(s)	171, 178
BspT107I	GGYRCC	1 cut(s)	169
BsrBI	CCGCTC	1 cut(s)	266
BsrI	ACTGG	1 cut(s)	280
BssECI	CCNNGG	1 cut(s)	255
BssHII	GCGCGC	1 cut(s)	213
Bst2UI	CCWGG	2 cut(s)	174, 256
BstAPI	GCANNNNNTGC	1 cut(s)	123
BstC8I	GCNNGC	2 cut(s)	215, 268
BstDEI	CTNAG	5 cut(s)	77, 85, 96, 111, 137
BstF5I	GGATG	2 cut(s)	5, 229
BstFNI	CGCG	2 cut(s)	134, 215
BstHHI	GCGC	4 cut(s)	136, 158, 215, 217
BstMAI	GTCTC	1 cut(s)	21
BstMCI	CGRYCG	1 cut(s)	132
BstMWI	GCNNNNNNNGC	3 cut(s)	123, 221, 260
BstNI	CCWGG	2 cut(s)	174, 256
BstSCI	CCNGG	2 cut(s)	172, 254
BstSLI	GKGCMC	1 cut(s)	93
BstUI	CGCG	2 cut(s)	134, 215
BstV1I	GCAGC	5 cut(s)	23, 34, 129, 153, 238
BstZI	CGGCCG	1 cut(s)	129
BsuRI	GGCC	1 cut(s)	131
BtsCI	GGATG	2 cut(s)	5, 229
BtsIMutI	CAGTG	2 cut(s)	93, 287
Cac8I	GCNNGC	2 cut(s)	215, 268
CfoI	GCGC	4 cut(s)	136, 158, 215, 217
CviJI	RGCY	8 cut(s)	36, 47, 81, 104, 131, 144, 177, 224
CviKI_1	RGCY	8 cut(s)	36, 47, 81, 104, 131, 144, 177, 224
DdeI	CTNAG	5 cut(s)	77, 85, 96, 111, 137
EaeI	YGGCCR	1 cut(s)	129
EagI	CGGCCG	1 cut(s)	129
EclXI	CGGCCG	1 cut(s)	129
Eco52I	CGGCCG	1 cut(s)	129
Eco57I	CTGAAG	1 cut(s)	16
EcoRII	CCWGG	2 cut(s)	172, 254
FauI	CCCGC	1 cut(s)	283
Fnu4HI	GCNGC	7 cut(s)	37, 48, 118, 132, 142, 252, 264
FokI	GGATG	1 cut(s)	216
Fsp4HI	GCNGC	7 cut(s)	37, 48, 118, 132, 142, 252, 264
FspAI	RTGCGCAY	1 cut(s)	157
FspI	TGCGCA	1 cut(s)	157
GlaI	GCGC	4 cut(s)	135, 157, 214, 216
GluI	GCNGC	7 cut(s)	37, 48, 118, 132, 142, 252, 264
HaeIII	GGCC	1 cut(s)	131
HhaI	GCGC	4 cut(s)	136, 158, 215, 217
Hin6I	GCGC	4 cut(s)	134, 156, 213, 215
HinP1I	GCGC	4 cut(s)	134, 156, 213, 215
Hpy166II	GTNNAC	1 cut(s)	91
Hpy188I	TCNGA	4 cut(s)	99, 114, 195, 202
Hpy188III	TCNNGA	1 cut(s)	233
Hpy8I	GTNNAC	1 cut(s)	91
HpyAV	CCTTC	3 cut(s)	19, 40, 94
HpyCH4V	TGCA	3 cut(s)	91, 246, 285
HpyF10VI	GCNNNNNNNGC	3 cut(s)	123, 221, 260
HpyF3I	CTNAG	5 cut(s)	77, 85, 96, 111, 137
HspAI	GCGC	4 cut(s)	134, 156, 213, 215
LmnI	GCTCC	2 cut(s)	182, 222
LpnPI	CCDG	7 cut(s)	89, 159, 186, 241, 246, 268, 293
Lsp1109I	GCAGC	5 cut(s)	23, 34, 129, 153, 238
LweI	GCATC	2 cut(s)	14, 97
MaeIII	GTNAC	1 cut(s)	305
MbiI	CCGCTC	1 cut(s)	266
MhlI	GDGCHC	1 cut(s)	93
MnlI	CCTC	2 cut(s)	92, 281
MspA1I	CMGCKG	1 cut(s)	36
MspR9I	CCNGG	2 cut(s)	174, 256
Mva1269I	GAATGC	1 cut(s)	242
MvaI	CCWGG	2 cut(s)	174, 256
MvnI	CGCG	2 cut(s)	134, 215
MwoI	GCNNNNNNNGC	3 cut(s)	123, 221, 260
NlaIV	GGNNCC	2 cut(s)	171, 178
NmeAIII	GCCGAG	1 cut(s)	107
NmuCI	GTSAC	1 cut(s)	305
NsbI	TGCGCA	1 cut(s)	157
PauI	GCGCGC	1 cut(s)	213
PctI	GAATGC	1 cut(s)	242
PkrI	GCNGC	7 cut(s)	38, 49, 119, 133, 143, 253, 265
Psp6I	CCWGG	2 cut(s)	172, 254
PspGI	CCWGG	2 cut(s)	172, 254
PspN4I	GGNNCC	2 cut(s)	171, 178
PteI	GCGCGC	1 cut(s)	213
PvuII	CAGCTG	1 cut(s)	36
SatI	GCNGC	7 cut(s)	37, 48, 118, 132, 142, 252, 264
ScrFI	CCNGG	2 cut(s)	174, 256
SduI	GDGCHC	1 cut(s)	93
SetI	ASST	3 cut(s)	38, 49, 163
SfaNI	GCATC	2 cut(s)	14, 97
SsiI	CCGC	3 cut(s)	132, 264, 276
StyD4I	CCNGG	2 cut(s)	172, 254
TauI	GCSGC	2 cut(s)	134, 266
TscAI	CASTG	2 cut(s)	93, 287
TseFI	GTSAC	1 cut(s)	305
TseI	GCWGC	5 cut(s)	36, 47, 117, 141, 251
Tsp45I	GTSAC	1 cut(s)	305
TspRI	CASTG	2 cut(s)	93, 287
VneI	GTGCAC	1 cut(s)	89

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.