RLG00000026568

Probable lipid transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016633
Physical Location & Seq
Reverse (-)
3880388 .. 3882424
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026568

Sequence Viewer

Length: 465 bp
ATGGCAGCCTTCAAGTCTATGATCAACTCCCAAGCTGCAGCACTACTGCTTGTGGCTTTGATGGGCCTCGCGCTGGTGCATATCCAGATGGCCGAGGCTCAGGACAGCTCGTGCGCTGCCGTCCTGACCAATCTGAATGTGTGTGCACCCTTTGTGGTTCCGGGGAGTAACGACAAACCAAGCTCCGACTGCTGCAATGCTGTCCAAGCTGCGGAAACGAGCTGTATTTGCAACACTCTCAGGATGGCTGCTCAACTTCCCTCTCAGTGCAATCTCCCTCCCCTCTCTTGTGGTGGTTACTTTTCTGGCATCGGAAACCGGAATCCAAATTCGACTGTTAAAGGCTATGGCTTTGCAGTTGCCTCACTCTTAAAGGAGGGGTCTTTCATCAGATGTGAAATTTGGCCACCTGGATTCGGCATTGAAAGGGGTCAATGCCAGAATCTGAGCTTGCTGTTTGTGTAA

Protein Analysis

155

Amino Acids

16.12

Weight (kDa)

6.51

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 26 - 83 2.6e-07 Probable lipid transfer
Tryp_alpha_amyl PF00234 38 - 96 3.8e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016633)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07230 AT5G52160 AT5G62080
fragaria_vesca FvH4_7g29091
malus_domestica MD01G1197500.v1.1
prunus_persica Prupe.2G290900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0376811
rosa_laevigata RLG00000026568
rosa_roxburghii Rroxscaffold_4G00281700
rosa_samantha Rh1AG415900 Rh1BG375300 Rh1CG389100 Rh1DG405900
rosa_wichuraiana Rw1G036480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 71
AciI CCGC 1 cut(s) 212
AcoI YGGCCR 2 cut(s) 90, 404
AcsI RAATTY 2 cut(s) 328, 399
AfiI CCNNNNNNNGG 3 cut(s) 73, 211, 416
AgsI TTSAA 2 cut(s) 13, 425
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 6 cut(s) 35, 108, 183, 209, 222, 450
AluI AGCT 6 cut(s) 35, 108, 183, 209, 222, 450
Alw21I GWGCWC 1 cut(s) 148
Alw44I GTGCAC 1 cut(s) 144
AlwNI CAGNNNCTG 1 cut(s) 445
AoxI GGCC 3 cut(s) 64, 90, 404
ApaLI GTGCAC 1 cut(s) 144
ApeKI GCWGC 7 cut(s) 5, 35, 38, 116, 192, 209, 248
ApoI RAATTY 2 cut(s) 328, 399
AspLEI GCGC 2 cut(s) 73, 116
AspS9I GGNCC 1 cut(s) 64
AsuC2I CCSGG 1 cut(s) 162
BaeGI GKGCMC 1 cut(s) 148
BalI TGGCCA 1 cut(s) 406
BauI CACGAG 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 7 cut(s) 17, 22, 50, 103, 179, 196, 235
BccI CCATC 3 cut(s) 55, 82, 238
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 1 cut(s) 411
BclI TGATCA 1 cut(s) 21
BcnI CCSGG 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 36
BisI GCNGC 7 cut(s) 6, 36, 39, 117, 193, 210, 249
BlsI GCNGC 7 cut(s) 7, 37, 40, 118, 194, 211, 250
Bme1390I CCNGG 2 cut(s) 162, 411
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 2 cut(s) 162, 411
BmsI GCATC 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 99
BpuMI CCSGG 1 cut(s) 162
BsaJI CCNNGG 2 cut(s) 93, 161
BsaWI WCCGGW 1 cut(s) 318
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 211, 416
Bse3DI GCAATG 1 cut(s) 202
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 2 cut(s) 93, 161
BseGI GGATG 1 cut(s) 249
BseLI CCNNNNNNNGG 3 cut(s) 73, 211, 416
BseMI GCAATG 1 cut(s) 202
BseMII CTCAG 4 cut(s) 113, 253, 278, 437
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 7 cut(s) 17, 22, 50, 103, 179, 196, 235
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 3 cut(s) 66, 92, 406
BsiHKAI GWGCWC 1 cut(s) 148
BsiSI CCGG 2 cut(s) 161, 319
BslI CCNNNNNNNGG 3 cut(s) 73, 211, 416
BsnI GGCC 3 cut(s) 66, 92, 406
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 1 cut(s) 21
BspACI CCGC 1 cut(s) 212
BspANI GGCC 3 cut(s) 66, 92, 406
BspCNI CTCAG 4 cut(s) 112, 252, 277, 438
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 1 cut(s) 159
BspMAI CTGCAG 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 93, 161
BssMI GATC 1 cut(s) 21
BssSI CACGAG 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 109
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 452
BstDEI CTNAG 4 cut(s) 99, 239, 264, 446
BstF5I GGATG 1 cut(s) 249
BstFNI CGCG 1 cut(s) 71
BstHHI GCGC 2 cut(s) 73, 116
BstKTI GATC 1 cut(s) 24
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 3 cut(s) 189, 206, 228
BstNI CCWGG 1 cut(s) 411
BstSCI CCNGG 2 cut(s) 160, 409
BstSFI CTRYAG 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 148
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 7 cut(s) 17, 22, 50, 103, 179, 196, 235
BsuRI GGCC 3 cut(s) 66, 92, 406
BtsCI GGATG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 452
CaiI CAGNNNCTG 1 cut(s) 445
CfoI GCGC 2 cut(s) 73, 116
Cfr13I GGNCC 1 cut(s) 64
DdeI CTNAG 4 cut(s) 99, 239, 264, 446
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
EaeI YGGCCR 2 cut(s) 90, 404
EcoRII CCWGG 1 cut(s) 409
FaiI YATR 3 cut(s) 20, 81, 348
FbaI TGATCA 1 cut(s) 21
Fnu4HI GCNGC 7 cut(s) 6, 36, 39, 117, 193, 210, 249
FokI GGATG 1 cut(s) 256
Fsp4HI GCNGC 7 cut(s) 6, 36, 39, 117, 193, 210, 249
GlaI GCGC 2 cut(s) 72, 115
GluI GCNGC 7 cut(s) 6, 36, 39, 117, 193, 210, 249
HaeIII GGCC 3 cut(s) 66, 92, 406
HapII CCGG 2 cut(s) 161, 319
HhaI GCGC 2 cut(s) 73, 116
Hin6I GCGC 2 cut(s) 71, 114
HinP1I GCGC 2 cut(s) 71, 114
HinfI GANTC 3 cut(s) 322, 414, 442
HpaII CCGG 2 cut(s) 161, 319
Hpy166II GTNNAC 1 cut(s) 146
Hpy188I TCNGA 5 cut(s) 135, 187, 314, 392, 447
Hpy188III TCNNGA 4 cut(s) 85, 101, 124, 241
Hpy8I GTNNAC 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4V TGCA 7 cut(s) 38, 79, 146, 195, 231, 270, 356
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 206, 228
HpyF3I CTNAG 4 cut(s) 99, 239, 264, 446
HspAI GCGC 2 cut(s) 71, 114
Ksp22I TGATCA 1 cut(s) 21
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 7 cut(s) 17, 22, 50, 103, 179, 196, 235
LweI GCATC 1 cut(s) 318
MaeIII GTNAC 2 cut(s) 167, 296
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MhlI GDGCHC 1 cut(s) 148
MlsI TGGCCA 1 cut(s) 406
MluCI AATT 2 cut(s) 328, 399
MluNI TGGCCA 1 cut(s) 406
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 7 cut(s) 77, 88, 271, 288, 293, 370, 373
Mox20I TGGCCA 1 cut(s) 406
MscI TGGCCA 1 cut(s) 406
MseI TTAA 2 cut(s) 339, 371
Msp20I TGGCCA 1 cut(s) 406
MspI CCGG 2 cut(s) 161, 319
MspR9I CCNGG 2 cut(s) 162, 411
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 3 cut(s) 189, 206, 228
NciI CCSGG 1 cut(s) 162
NdeII GATC 1 cut(s) 21
NlaIV GGNNCC 1 cut(s) 159
NmeAIII GCCGAG 1 cut(s) 118
PfeI GAWTC 3 cut(s) 322, 414, 442
PkrI GCNGC 7 cut(s) 7, 37, 40, 118, 194, 211, 250
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 1 cut(s) 64
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 445
SaqAI TTAA 2 cut(s) 339, 371
SatI GCNGC 7 cut(s) 6, 36, 39, 117, 193, 210, 249
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 1 cut(s) 64
ScrFI CCNGG 2 cut(s) 162, 411
SduI GDGCHC 1 cut(s) 148
SetI ASST 7 cut(s) 37, 110, 185, 211, 224, 412, 452
SfaNI GCATC 1 cut(s) 318
SfcI CTRYAG 1 cut(s) 36
Sse9I AATT 2 cut(s) 328, 399
SsiI CCGC 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 160, 409
TaaI ACNGT 1 cut(s) 337
TaqI TCGA 1 cut(s) 332
TasI AATT 2 cut(s) 328, 399
TfiI GAWTC 3 cut(s) 322, 414, 442
Tru1I TTAA 2 cut(s) 339, 371
Tru9I TTAA 2 cut(s) 339, 371
TscAI CASTG 1 cut(s) 272
TseI GCWGC 7 cut(s) 5, 35, 38, 116, 192, 209, 248
TspDTI ATGAA 1 cut(s) 376
TspRI CASTG 1 cut(s) 272
VneI GTGCAC 1 cut(s) 144
XapI RAATTY 2 cut(s) 328, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.