Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0013248

Physical Location & Seq

Genomic Coordinates Reverse (-)

29762168 .. 29763586

Gene Structure 1419 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1201300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 414 bp

ATGTCTATTGTGGAGGTGAGAGTTCCAAACCTTGATTGTGAAGGATGTGCTTCCAAGTTGAAGAAAGCTCTCTCCAAACTGAAAGGCGTGGAAGAGGTGGAAGTAGAAATGGAGATCCAGAAAATAATAGTGAGGGGATATGCATTGGAGGAAAAGAAAGTGGTGAAGGCAATTAAGAAAGCTGGGAAAGCAGCAGAGCCATGGCCTTTCCCTGGATACTCTCACTTTGCCTCATTTTACAAGTACCCCACCTACATTGTCAACCATTACTATGACACTTACAAGAACCAAACCACCACCGGCGTCCACACCTTCTTCCACACTCCGTCGGTTTATTCCGTCGCCGTCGCATCCGACGAGGCCATTGCTTCGCTTTTTAGCGACGACAATCCGCATGCTTGTGCTATCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0008150 GO:0009987 GO:0019725 GO:0030001 GO:0030003 GO:0042592 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

138

Amino Acids

15.33

Weight (kDa)

6.95

Isoelectric Point (pI)

26.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	6 - 63	8.5e-14	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013248)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48970 AT3G48970 AT3G48970
fragaria_vesca	FvH4_7g29520
malus_domestica	MD01G1201300.v1.1
prunus_persica	Prupe.2G294700_v2.0.a1
pyrus_communis	pycom01g21100
rosa_chinensis	RchiOBHm_Chr1g0377361
rosa_laevigata	RLG00000026525
rosa_multiflora	Rmu_co8119758.1_g000001
rosa_roxburghii	Rroxscaffold_4G00281280
rosa_rugosa	Rorug01G0403000 Rorug01G0403100
rosa_samantha	Rh1AG420800 Rh1BG379600 Rh1CG393600 Rh1DG410600
rosa_wichuraiana	Rw1G036890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	392
AclWI	GGATC	1 cut(s)	109
AcyI	GRCGYC	1 cut(s)	303
AfaI	GTAC	1 cut(s)	245
AfiI	CCNNNNNNNGG	1 cut(s)	212
AgsI	TTSAA	1 cut(s)	61
AjnI	CCWGG	1 cut(s)	211
AluBI	AGCT	2 cut(s)	68, 182
AluI	AGCT	2 cut(s)	68, 182
AlwI	GGATC	1 cut(s)	109
AoxI	GGCC	2 cut(s)	203, 360
ApeKI	GCWGC	1 cut(s)	191
AsuHPI	GGTGA	2 cut(s)	28, 175
BbvI	GCAGC	1 cut(s)	203
BceAI	ACGGC	1 cut(s)	329
BcgI	CGANNNNNNTGC	2 cut(s)	347, 381
BciT130I	CCWGG	1 cut(s)	213
BciVI	GTATCC	1 cut(s)	209
BfuI	GTATCC	1 cut(s)	209
BisI	GCNGC	1 cut(s)	192
BlsI	GCNGC	1 cut(s)	193
Bme1390I	CCNGG	1 cut(s)	213
BmrFI	CCNGG	1 cut(s)	213
BmsI	GCATC	1 cut(s)	359
BsaHI	GRCGYC	1 cut(s)	303
BsaJI	CCNNGG	2 cut(s)	200, 211
Bsc4I	CCNNNNNNNGG	1 cut(s)	212
Bse118I	RCCGGY	1 cut(s)	299
Bse3DI	GCAATG	1 cut(s)	363
BseBI	CCWGG	1 cut(s)	213
BseDI	CCNNGG	2 cut(s)	200, 211
BseGI	GGATG	2 cut(s)	50, 350
BseLI	CCNNNNNNNGG	1 cut(s)	212
BseMI	GCAATG	1 cut(s)	363
BseXI	GCAGC	1 cut(s)	203
BseYI	CCCAGC	1 cut(s)	182
BshFI	GGCC	2 cut(s)	205, 362
BsiSI	CCGG	1 cut(s)	300
BslI	CCNNNNNNNGG	1 cut(s)	212
BsnI	GGCC	2 cut(s)	205, 362
Bsp143I	GATC	1 cut(s)	114
Bsp19I	CCATGG	1 cut(s)	200
BspACI	CCGC	1 cut(s)	392
BspANI	GGCC	2 cut(s)	205, 362
BspPI	GGATC	1 cut(s)	109
BsrDI	GCAATG	1 cut(s)	363
BsrFI	RCCGGY	1 cut(s)	299
BssAI	RCCGGY	1 cut(s)	299
BssECI	CCNNGG	2 cut(s)	200, 211
BssMI	GATC	1 cut(s)	114
BssNI	GRCGYC	1 cut(s)	303
BssT1I	CCWWGG	1 cut(s)	200
Bst2UI	CCWGG	1 cut(s)	213
Bst6I	CTCTTC	1 cut(s)	87
BstACI	GRCGYC	1 cut(s)	303
BstC8I	GCNNGC	1 cut(s)	396
BstDSI	CCRYGG	1 cut(s)	200
BstF5I	GGATG	2 cut(s)	50, 350
BstKTI	GATC	1 cut(s)	117
BstMBI	GATC	1 cut(s)	114
BstMWI	GCNNNNNNNGC	1 cut(s)	188
BstNI	CCWGG	1 cut(s)	213
BstNSI	RCATGY	1 cut(s)	398
BstSCI	CCNGG	1 cut(s)	211
BstV1I	GCAGC	1 cut(s)	203
BstX2I	RGATCY	1 cut(s)	114
BstYI	RGATCY	1 cut(s)	114
BsuI	GTATCC	1 cut(s)	209
BsuRI	GGCC	2 cut(s)	205, 362
BtgI	CCRYGG	1 cut(s)	200
BtsCI	GGATG	2 cut(s)	50, 350
Cac8I	GCNNGC	1 cut(s)	396
Cfr10I	RCCGGY	1 cut(s)	299
CseI	GACGC	1 cut(s)	292
Csp6I	GTAC	1 cut(s)	244
CspCI	CAANNNNNGTGG	2 cut(s)	238, 273
CviAII	CATG	3 cut(s)	201, 395, 409
CviJI	RGCY	5 cut(s)	68, 182, 199, 205, 362
CviKI_1	RGCY	5 cut(s)	68, 182, 199, 205, 362
CviQI	GTAC	1 cut(s)	244
DpnI	GATC	1 cut(s)	116
DpnII	GATC	1 cut(s)	114
Eam1104I	CTCTTC	1 cut(s)	87
EarI	CTCTTC	1 cut(s)	87
Eco130I	CCWWGG	1 cut(s)	200
EcoRII	CCWGG	1 cut(s)	211
EcoT14I	CCWWGG	1 cut(s)	200
EcoT22I	ATGCAT	1 cut(s)	145
ErhI	CCWWGG	1 cut(s)	200
FaeI	CATG	3 cut(s)	204, 398, 412
FaiI	YATR	5 cut(s)	141, 202, 273, 396, 410
FatI	CATG	3 cut(s)	200, 394, 408
Fnu4HI	GCNGC	1 cut(s)	192
FokI	GGATG	2 cut(s)	57, 337
Fsp4HI	GCNGC	1 cut(s)	192
GluI	GCNGC	1 cut(s)	192
GsaI	CCCAGC	1 cut(s)	186
HaeIII	GGCC	2 cut(s)	205, 362
HapII	CCGG	1 cut(s)	300
HgaI	GACGC	1 cut(s)	292
Hin1I	GRCGYC	1 cut(s)	303
Hin1II	CATG	3 cut(s)	204, 398, 412
HincII	GTYRAC	1 cut(s)	262
HindII	GTYRAC	1 cut(s)	262
HpaII	CCGG	1 cut(s)	300
HphI	GGTGA	2 cut(s)	28, 175
Hpy166II	GTNNAC	2 cut(s)	262, 307
Hpy188I	TCNGA	1 cut(s)	355
Hpy188III	TCNNGA	1 cut(s)	118
Hpy8I	GTNNAC	2 cut(s)	262, 307
Hpy99I	CGWCG	5 cut(s)	331, 344, 350, 359, 386
HpyAV	CCTTC	3 cut(s)	35, 160, 322
HpyCH4V	TGCA	1 cut(s)	143
HpyF10VI	GCNNNNNNNGC	1 cut(s)	188
Hsp92I	GRCGYC	1 cut(s)	303
Hsp92II	CATG	3 cut(s)	204, 398, 412
Kzo9I	GATC	1 cut(s)	114
LpnPI	CCDG	5 cut(s)	131, 168, 198, 225, 313
Lsp1109I	GCAGC	1 cut(s)	203
LweI	GCATC	1 cut(s)	359
MalI	GATC	1 cut(s)	116
MboI	GATC	1 cut(s)	114
MboII	GAAGA	3 cut(s)	73, 104, 307
MflI	RGATCY	1 cut(s)	114
MluCI	AATT	1 cut(s)	171
MmeI	TCCRAC	1 cut(s)	378
MnlI	CCTC	6 cut(s)	7, 88, 126, 142, 241, 352
Mph1103I	ATGCAT	1 cut(s)	145
MseI	TTAA	1 cut(s)	174
MslI	CAYNNNNRTG	2 cut(s)	270, 399
MspI	CCGG	1 cut(s)	300
MspR9I	CCNGG	1 cut(s)	213
MvaI	CCWGG	1 cut(s)	213
MwoI	GCNNNNNNNGC	1 cut(s)	188
NcoI	CCATGG	1 cut(s)	200
NdeII	GATC	1 cut(s)	114
NlaIII	CATG	3 cut(s)	204, 398, 412
NsiI	ATGCAT	1 cut(s)	145
NspI	RCATGY	1 cut(s)	398
PaeI	GCATGC	1 cut(s)	398
PcsI	WCGNNNNNNNCGW	1 cut(s)	354
PkrI	GCNGC	1 cut(s)	193
Psp6I	CCWGG	1 cut(s)	211
PspFI	CCCAGC	1 cut(s)	182
PspGI	CCWGG	1 cut(s)	211
PsuI	RGATCY	1 cut(s)	114
RsaI	GTAC	1 cut(s)	245
RsaNI	GTAC	1 cut(s)	244
RseI	CAYNNNNRTG	2 cut(s)	270, 399
SaqAI	TTAA	1 cut(s)	174
SatI	GCNGC	1 cut(s)	192
Sau3AI	GATC	1 cut(s)	114
ScrFI	CCNGG	1 cut(s)	213
SetI	ASST	7 cut(s)	18, 33, 70, 99, 184, 254, 314
SfaNI	GCATC	1 cut(s)	359
SgrAI	CRCCGGYG	1 cut(s)	299
SmiMI	CAYNNNNRTG	2 cut(s)	270, 399
SphI	GCATGC	1 cut(s)	398
Sse9I	AATT	1 cut(s)	171
SsiI	CCGC	1 cut(s)	392
StyD4I	CCNGG	1 cut(s)	211
StyI	CCWWGG	1 cut(s)	200
TasI	AATT	1 cut(s)	171
Tru1I	TTAA	1 cut(s)	174
Tru9I	TTAA	1 cut(s)	174
TseI	GCWGC	1 cut(s)	191
TspGWI	ACGGA	2 cut(s)	315, 328
XceI	RCATGY	1 cut(s)	398
Zsp2I	ATGCAT	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.