RLG00000026525

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013248
Physical Location & Seq
Forward (+)
3576463 .. 3577095
633 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026525

Sequence Viewer

Length: 414 bp
ATGTCTATGGTGGAGGTGAGAGTTCCAAATCTTGACTGTCAGGGATGTGCTTCCAAGTTAAAGAAAGCTCTCTTCAAGATCAAAGGTGTCGAAGTAGTGGAAATAGAGATGGAGATCCAAAAGATAAGCGTGAGAGGGTACGCGTTGGATGAAAGGAAAGTGCTAAAGGCAATTAAGCGAGCTGGAAAAGCAGTGGAGCCATGGCCATTTCCTGGTTACTCACATTATGCCTCATTTTACAAGTACCCTACATACATTAAGAACCATTACTACGACACCTACAAAAACGAAACCACCACCGGCGTACACACCTTCTTCCACACTCCGGCAGTTTATTCAGTCGCCGTGGCATCCGATGAGGCCATTGCTTCACTCTTTAGTGATGACAATCCACATGCTTGCACTATTATGTGA

Protein Analysis

138

Amino Acids

15.51

Weight (kDa)

8.36

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 6 - 63 8.1e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48970 AT3G48970 AT3G48970
fragaria_vesca FvH4_7g29520
malus_domestica MD01G1201300.v1.1
prunus_persica Prupe.2G294700_v2.0.a1
pyrus_communis pycom01g21100
rosa_chinensis RchiOBHm_Chr1g0377361
rosa_laevigata RLG00000026525
rosa_multiflora Rmu_co8119758.1_g000001
rosa_roxburghii Rroxscaffold_4G00281280
rosa_rugosa Rorug01G0403000 Rorug01G0403100
rosa_samantha Rh1AG420800 Rh1BG379600 Rh1CG393600 Rh1DG410600
rosa_wichuraiana Rw1G036890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 212
AccII CGCG 1 cut(s) 143
AclWI GGATC 1 cut(s) 109
AcoI YGGCCR 1 cut(s) 203
AfaI GTAC 3 cut(s) 140, 245, 306
AfiI CCNNNNNNNGG 2 cut(s) 212, 325
AflIII ACRYGT 1 cut(s) 141
AgsI TTSAA 1 cut(s) 76
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 2 cut(s) 68, 182
AluI AGCT 2 cut(s) 68, 182
AlwI GGATC 1 cut(s) 109
AoxI GGCC 2 cut(s) 203, 360
AsuHPI GGTGA 1 cut(s) 28
BalI TGGCCA 1 cut(s) 205
BccI CCATC 1 cut(s) 103
BceAI ACGGC 1 cut(s) 329
BciT130I CCWGG 1 cut(s) 213
Bme1390I CCNGG 1 cut(s) 213
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 1 cut(s) 359
BsaBI GATNNNNATC 2 cut(s) 113, 387
BsaJI CCNNGG 2 cut(s) 200, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 325
Bse118I RCCGGY 1 cut(s) 299
Bse3DI GCAATG 1 cut(s) 363
Bse8I GATNNNNATC 2 cut(s) 113, 387
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 200, 345
BseGI GGATG 3 cut(s) 50, 154, 350
BseJI GATNNNNATC 2 cut(s) 113, 387
BseLI CCNNNNNNNGG 2 cut(s) 212, 325
BseMI GCAATG 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 2 cut(s) 205, 362
BsiSI CCGG 2 cut(s) 300, 326
BslI CCNNNNNNNGG 2 cut(s) 212, 325
BsnI GGCC 2 cut(s) 205, 362
Bsp143I GATC 2 cut(s) 78, 114
Bsp19I CCATGG 1 cut(s) 200
BspANI GGCC 2 cut(s) 205, 362
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 1 cut(s) 198
BspPI GGATC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 363
BsrFI RCCGGY 1 cut(s) 299
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 2 cut(s) 200, 345
BssMI GATC 2 cut(s) 78, 114
BssT1I CCWWGG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 38
Bst6I CTCTTC 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 180, 400
BstDSI CCRYGG 2 cut(s) 200, 345
BstF5I GGATG 3 cut(s) 50, 154, 350
BstFNI CGCG 1 cut(s) 143
BstKTI GATC 2 cut(s) 81, 117
BstMBI GATC 2 cut(s) 78, 114
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 211
BstUI CGCG 1 cut(s) 143
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
BsuRI GGCC 2 cut(s) 205, 362
BtgI CCRYGG 2 cut(s) 200, 345
BtsCI GGATG 3 cut(s) 50, 154, 350
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 180, 400
Cfr10I RCCGGY 1 cut(s) 299
Csp6I GTAC 3 cut(s) 139, 244, 305
CviAII CATG 2 cut(s) 201, 395
CviJI RGCY 5 cut(s) 68, 182, 199, 205, 362
CviKI_1 RGCY 5 cut(s) 68, 182, 199, 205, 362
CviQI GTAC 3 cut(s) 139, 244, 305
DpnI GATC 2 cut(s) 80, 116
DpnII GATC 2 cut(s) 78, 114
EaeI YGGCCR 1 cut(s) 203
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 2 cut(s) 204, 398
FaiI YATR 6 cut(s) 8, 202, 228, 253, 396, 410
FatI CATG 2 cut(s) 200, 394
FokI GGATG 3 cut(s) 57, 161, 337
HaeIII GGCC 2 cut(s) 205, 362
HapII CCGG 2 cut(s) 300, 326
Hin1II CATG 2 cut(s) 204, 398
HpaII CCGG 2 cut(s) 300, 326
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 1 cut(s) 355
Hpy188III TCNNGA 2 cut(s) 32, 76
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 1 cut(s) 322
HpyCH4III ACNGT 1 cut(s) 38
HpyCH4V TGCA 1 cut(s) 402
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
Hsp92II CATG 2 cut(s) 204, 398
Kzo9I GATC 2 cut(s) 78, 114
LmnI GCTCC 1 cut(s) 196
LpnPI CCDG 6 cut(s) 26, 168, 198, 225, 313, 339
LweI GCATC 1 cut(s) 359
MaeIII GTNAC 1 cut(s) 215
MalI GATC 2 cut(s) 80, 116
MboI GATC 2 cut(s) 78, 114
MboII GAAGA 2 cut(s) 64, 307
MflI RGATCY 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 205
MluCI AATT 1 cut(s) 171
MluI ACGCGT 1 cut(s) 141
MluNI TGGCCA 1 cut(s) 205
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 4 cut(s) 7, 128, 241, 352
Mox20I TGGCCA 1 cut(s) 205
MscI TGGCCA 1 cut(s) 205
MseI TTAA 3 cut(s) 59, 174, 258
MslI CAYNNNNRTG 1 cut(s) 407
Msp20I TGGCCA 1 cut(s) 205
MspI CCGG 2 cut(s) 300, 326
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 188
NcoI CCATGG 1 cut(s) 200
NdeII GATC 2 cut(s) 78, 114
NlaIII CATG 2 cut(s) 204, 398
NlaIV GGNNCC 1 cut(s) 198
NspI RCATGY 1 cut(s) 398
PflMI CCANNNNNTGG 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 1 cut(s) 198
PsrI GAACNNNNNNTAC 2 cut(s) 254, 286
PsuI RGATCY 1 cut(s) 114
RsaI GTAC 3 cut(s) 140, 245, 306
RsaNI GTAC 3 cut(s) 139, 244, 305
RseI CAYNNNNRTG 1 cut(s) 407
SaqAI TTAA 3 cut(s) 59, 174, 258
Sau3AI GATC 2 cut(s) 78, 114
ScrFI CCNGG 1 cut(s) 213
SetI ASST 6 cut(s) 18, 70, 88, 184, 281, 314
SfaNI GCATC 1 cut(s) 359
SgrAI CRCCGGYG 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 407
Sse9I AATT 1 cut(s) 171
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 1 cut(s) 38
TaqI TCGA 1 cut(s) 90
TasI AATT 1 cut(s) 171
Tru1I TTAA 3 cut(s) 59, 174, 258
Tru9I TTAA 3 cut(s) 59, 174, 258
TscAI CASTG 1 cut(s) 198
TspDTI ATGAA 1 cut(s) 165
TspRI CASTG 1 cut(s) 198
Van91I CCANNNNNTGG 1 cut(s) 212
XceI RCATGY 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.