MD02G1008100.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016668
Physical Location & Seq
Reverse (-)
508606 .. 511191
2586 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1008100.v1.1.491

Sequence Viewer

Length: 2586 bp
ATGGGTGTTCATTTCTTTGCTTCTCTACTGGTTTTTTCACTGTTCCTGAATCCATTGGCTTCCCAACAACCCAATACAGATGGGTTCTTTGTCTCTGAGTTTTTGAAAAAAATGGGTTCGTTTTCTTCATCTTCTTCTTCCAGTCTCTACGACTTCTCTGCACCTGTTTGTTCATGGAAAGGAGTTTCTTGTGAATCCAACGATGTTATCGGCTTTGAGGCTTCCGGGTTGGAGCTCTCCGGTCCGGTTCCCGACACCACCATCGGAAAGCTCACAAAGCTTCAAACTTTGAACCTCAGCCGCAACAAAATCACCGGTCTTCCTTCGGATTTGTGGAGCATAAGTTCTCTCAAGCACCTTATTCTCTCCAACAACCAGATTTCCGGGTCGTTGCCGAACAACATTGGCAACTTCGGTCTGCTCGAAACCCTCGACGTTTCCAGCAATAATTTCTCTGGGGAGATTCCTGCTGGTATAAGCTCACTTGCCAGCCTGCGAGTTCTTAAGCTCAATGGAAATTCGTTTGAGAAGAGCATCCCATCAGGAATTCTGAGCTGTCAATCTTTGGTTTGGATTGACCTTTCGTCGAATCGGTTAAACGGTTCTCTTCCAGCTGGTTTTGGTGCTGCATTGCCTAAGCTGCAAGTATTGAACCTTGCTGGGAATGGGATTCATGGCCGGGGCTCCGATTTTTCGGAGATGAATTCCATTACTACTCTGAACATTTCAGGGAATGTGTTTCAGGGTTCAGTGATGGGAGTGTTTAAGGAGCTGTTGGAGGTGGCTGACCTTAGCAGGAACCGGTTTGAAGGCCACATTTCGAAGGTACAATTCAATGCTAGTTACAATTGGTCTCGTTTGGTTTATTTAGATTTGTCCGAAAATCAGCTTAATGGAGAAATTTTACATCATTGGAATCAATCCCAGAATCTCAAACACCTTAATCTAGCACACAATAGATTTACTAGGCAAGAATTCCCGAGAAGTGTGGAAACTTTTTCTGGTTTGGAGTACCTTAACTTGTCGAAATCTAGTCTTACCAGTCGCATTCCTGCTGAAATTTCTCGGCTGAGTAACTTGAACACACTTGATCTTTCTGAAAACCATCTTATAGGCAAAATCCCATTGCTTAATTTGAGAAATCTCCAAGTTTTCGATGTTTCTCACAACAATTTGAGCGGAGAAATCCCTGTGTCTCTCTTGGAGAAACTCCCTTGGATGGAGAGCTTCAACTTCTCTTACAATAACTTGACACTATGTACTTCTGGAATTTCCAATGCAACCCTTCAAGCATCTTTCTTTGGTTCAACAAACAGCTGCCCCATTGCAGCAAACCCGGGCCCTTTTGGAGCACGAGCTAATAAGGCTAAGGAGCACAAGGTACTAAAGCTTGCCTTGGTTTTGACCTTCTCAATGGTATTCTTGCTAGCTGGTCTGCTGTTTCTAGCAGTTTGTTGCAGAAGGAAAACCAGAAGGTGGGAGGTAAAACAAACCTCGCACAAGGAAGAGCAAAGCGTATCAGGCCCCTTTTCCTTCCACACTGATTCAACAACATGGGTTGCAGATGTTAAGCAAGCAAATTCAGTACCTGTAGTGATTTTCGAGAAGCCATTGCTGAATTTCACATTCGCAGATCTCTTATCTGCAACTTCGAACTTTGATCGAGGAACCCTTTTGGCTGAAGGGAAATTCGGGCCTGTTTATAGAGGATTTCTACCGGGTGGAATTCATGTAGCGGTGAAAGTTTTGGTCCACGGTTCTACACTGACAGACCAGGAAGCTGCAAGAGAGTTTGAGTATCTCGGACGAATCAAACACCCTAATCTTGCTCCATTGACGGGATATTGTTTAGCTGGGGACCAAAGGATTGCTATCTATGACTACATGGAGAATGGGAATATACAGAATTTGCTACATGACTTGCCGCTTGGGGTACTGCAAACAACAGAGGATTGGAGCACAGATACCTGGGAAGAAGACAATAACAATGGCATTCAAAATGTTGGCTCTGAAGGGTTGTTAACATCATGGGGGTTCCGTCACAAGATTGCACTTGGCACTGCCAGAGCACTGGCATTTCTACACCACGGTTGCTCTCCTCCAATTATTCATAGAGATGTTAAAGCTAGTAGTGTTTATCTGGACTATAACTTAGAGCCAAGATTATCTGATTTTGGACTGGCAAAGATATTTGGCAGCGGTATGGATGAGGAGATCTCTCGAGGGTCACCTGGCTACATGCCGCCAGAGTTTTCTCAGTCCGAGTATGATTCTCCAACGCCAAAATCTGATGTGTATTGCTTTGGAGTTGTTCTTTTTGAGCTGATTACTTCCAAGAAGCCATTTGGCGATGACTATCCCGAGGAGAAAGATGCAACCTTGGTGAGCTGGGTTAGAGGACTGATTAGGAAGAACAAAGGGACGAGCGCTATTGATCCAAAAGTTCGTGACACAGGACCAGATGACCAAATGGAGGAGGCCCTGAAGATTGGATATCTGTGCACAGCTGACCTTCCCTCAAAACGACCAAGCATGCATCAGATAGTCGGACTTCTTAAAGATATCGAGCAAACAGGTAACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

862

Amino Acids

94.58

Weight (kDa)

6.15

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 83 - 127 1.1e-08 Leucine rich repeat
LRR_14 PF23598 87 - 179 8.1e-11 Leucine-rich repeat region
LRR_14 PF23598 110 - 194 2.4e-08 Leucine-rich repeat region
LRR_8 PF13855 141 - 199 7.5e-06 Leucine rich repeat
LRR_8 PF13855 188 - 245 1.5e-06 Leucine rich repeat
Pkinase PF00069 556 - 849 4.7e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 557 - 850 1.6e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016668)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24230
fragaria_vesca FvH4_1g01000
malus_domestica MD02G1008100.v1.1 MD15G1152400.v1.1
prunus_persica Prupe.7G262300_v2.0.a1
pyrus_communis pycom15g13700
rosa_chinensis RchiOBHm_Chr2g0085731
rosa_laevigata RLG00000015715
rosa_multiflora Rmu_sc0009759.1_g000005 Rmu_sc0023131.1_g000002 Rmu_sc0033740.1_g000001
rosa_rugosa Rorug01G0462800
rosa_samantha Rh2CG013300
rosa_wichuraiana Rw2G001060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1476
AccBSI CCGCTC 1 cut(s) 1179
AciI CCGC 6 cut(s) 301, 1179, 1736, 1925, 2199, 2243
AclWI GGATC 1 cut(s) 2429
AcoI YGGCCR 1 cut(s) 676
AcuI CTGAAG 3 cut(s) 1701, 2031, 2504
AfaI GTAC 6 cut(s) 828, 1013, 1261, 1383, 1587, 1935
AfeI AGCGCT 1 cut(s) 2428
AfiI CCNNNNNNNGG 4 cut(s) 1219, 1476, 1838, 2473
AflII CTTAAG 1 cut(s) 503
AgeI ACCGGT 2 cut(s) 314, 801
AhdI GACNNNNNGTC 1 cut(s) 583
AjnI CCWGG 3 cut(s) 1773, 1967, 2230
AloI GAACNNNNNNTCC 2 cut(s) 328, 360
Alw21I GWGCWC 6 cut(s) 237, 1354, 1377, 1961, 2071, 2504
Alw26I GTCTC 4 cut(s) 97, 149, 858, 1200
Alw44I GTGCAC 1 cut(s) 2500
AlwI GGATC 1 cut(s) 2429
AlwNI CAGNNNCTG 1 cut(s) 1589
Ama87I CYCGRG 4 cut(s) 979, 1336, 2220, 2360
Aor51HI AGCGCT 1 cut(s) 2428
AoxI GGCC 6 cut(s) 676, 811, 1339, 1522, 1694, 2478
ApaI GGGCCC 1 cut(s) 1343
ApaLI GTGCAC 1 cut(s) 2500
ApeKI GCWGC 6 cut(s) 626, 640, 1317, 1328, 1781, 2196
AsiGI ACCGGT 2 cut(s) 314, 801
AspLEI GCGC 1 cut(s) 2429
AspS9I GGNCC 9 cut(s) 242, 1339, 1340, 1523, 1694, 1750, 1858, 2456, 2479
AsuC2I CCSGG 6 cut(s) 226, 385, 680, 1337, 1338, 1719
AsuHPI GGTGA 4 cut(s) 304, 1750, 2220, 2395
AsuII TTCGAA 2 cut(s) 821, 1652
AsuNHI GCTAGC 1 cut(s) 1426
AvaI CYCGRG 4 cut(s) 979, 1336, 2220, 2360
AvaII GGWCC 4 cut(s) 242, 1750, 1858, 2456
BaeGI GKGCMC 2 cut(s) 1343, 2504
BanII GRGCYC 3 cut(s) 237, 686, 1343
BauI CACGAG 1 cut(s) 1353
BbsI GAAGAC 2 cut(s) 311, 1983
Bbv12I GWGCWC 6 cut(s) 237, 1354, 1377, 1961, 2071, 2504
BbvCI CCTCAGC 1 cut(s) 296
BbvI GCAGC 6 cut(s) 613, 627, 1304, 1340, 1768, 2208
BccI CCATC 6 cut(s) 74, 269, 547, 748, 1113, 1213
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 3 cut(s) 1775, 1969, 2232
BcnI CCSGG 6 cut(s) 226, 385, 680, 1337, 1338, 1719
BcoDI GTCTC 4 cut(s) 97, 149, 858, 1200
BfaI CTAG 7 cut(s) 840, 947, 966, 1032, 1427, 1445, 2127
BfmI CTRYAG 1 cut(s) 1590
BfoI RGCGCY 1 cut(s) 2430
BfrI CTTAAG 1 cut(s) 503
BglII AGATCT 2 cut(s) 1633, 2214
BisI GCNGC 9 cut(s) 301, 627, 641, 1318, 1329, 1782, 1925, 2197, 2243
BlsI GCNGC 9 cut(s) 302, 628, 642, 1319, 1330, 1783, 1926, 2198, 2244
Bme1390I CCNGG 9 cut(s) 226, 385, 680, 1337, 1338, 1719, 1775, 1969, 2232
Bme18I GGWCC 4 cut(s) 242, 1750, 1858, 2456
BmeRI GACNNNNNGTC 1 cut(s) 583
BmeT110I CYCGRG 4 cut(s) 979, 1336, 2220, 2360
BmgT120I GGNCC 9 cut(s) 242, 1339, 1340, 1523, 1694, 1750, 1858, 2456, 2479
BmiI GGNNCC 8 cut(s) 249, 685, 800, 1341, 1525, 1669, 1859, 2036
BmrFI CCNGG 9 cut(s) 226, 385, 680, 1337, 1338, 1719, 1775, 1969, 2232
BmsI GCATC 4 cut(s) 543, 1301, 2362, 2545
BmtI GCTAGC 1 cut(s) 1430
BpiI GAAGAC 2 cut(s) 311, 1983
BplI GAGNNNNNCTC 2 cut(s) 2201, 2233
Bpu10I CCTNAGC 4 cut(s) 296, 636, 791, 1368
Bpu14I TTCGAA 2 cut(s) 821, 1652
BpuEI CTTGAG 1 cut(s) 335
BpuMI CCSGG 6 cut(s) 226, 385, 680, 1337, 1338, 1719
BsaBI GATNNNNATC 2 cut(s) 1871, 2355
BsaI GGTCTC 1 cut(s) 858
BsaJI CCNNGG 9 cut(s) 679, 1214, 1336, 1395, 1753, 1968, 2086, 2361, 2379
BsaWI WCCGGW 4 cut(s) 239, 244, 314, 801
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 4 cut(s) 1219, 1476, 1838, 2473
Bse118I RCCGGY 2 cut(s) 314, 801
Bse1I ACTGG 5 cut(s) 33, 141, 1041, 2076, 2184
Bse3DI GCAATG 4 cut(s) 629, 1124, 1323, 1610
Bse8I GATNNNNATC 2 cut(s) 1871, 2355
BseBI CCWGG 3 cut(s) 1775, 1969, 2232
BseDI CCNNGG 9 cut(s) 679, 1214, 1336, 1395, 1753, 1968, 2086, 2361, 2379
BseGI GGATG 3 cut(s) 534, 1224, 2212
BseJI GATNNNNATC 2 cut(s) 1871, 2355
BseLI CCNNNNNNNGG 4 cut(s) 1219, 1476, 1838, 2473
BseMI GCAATG 4 cut(s) 629, 1124, 1323, 1610
BseMII CTCAG 5 cut(s) 87, 310, 542, 1061, 2270
BseNI ACTGG 5 cut(s) 33, 141, 1041, 2076, 2184
BseRI GAGGAG 4 cut(s) 2088, 2225, 2378, 2489
BseSI GKGCMC 2 cut(s) 1343, 2504
BseXI GCAGC 6 cut(s) 613, 627, 1304, 1340, 1768, 2208
BseYI CCCAGC 3 cut(s) 659, 1853, 2388
BsgI GTGCAG 1 cut(s) 144
BshFI GGCC 6 cut(s) 678, 813, 1341, 1524, 1696, 2480
BshTI ACCGGT 2 cut(s) 314, 801
BsiHKAI GWGCWC 6 cut(s) 237, 1354, 1377, 1961, 2071, 2504
BsiHKCI CYCGRG 4 cut(s) 979, 1336, 2220, 2360
BsiSI CCGG 9 cut(s) 225, 240, 245, 315, 384, 679, 802, 1337, 1718
BslFI GGGAC 2 cut(s) 1871, 2434
BslI CCNNNNNNNGG 4 cut(s) 1219, 1476, 1838, 2473
BsmAI GTCTC 4 cut(s) 97, 149, 858, 1200
BsmFI GGGAC 2 cut(s) 1871, 2434
BsmI GAATGC 2 cut(s) 1047, 1992
BsnI GGCC 6 cut(s) 678, 813, 1341, 1524, 1696, 2480
Bso31I GGTCTC 1 cut(s) 858
BsoBI CYCGRG 4 cut(s) 979, 1336, 2220, 2360
Bsp119I TTCGAA 2 cut(s) 821, 1652
Bsp120I GGGCCC 1 cut(s) 1339
Bsp1286I GDGCHC 8 cut(s) 237, 686, 1343, 1354, 1377, 1961, 2071, 2504
Bsp143I GATC 5 cut(s) 1090, 1633, 1660, 2214, 2434
BspACI CCGC 6 cut(s) 301, 1179, 1736, 1925, 2199, 2243
BspANI GGCC 6 cut(s) 678, 813, 1341, 1524, 1696, 2480
BspCNI CTCAG 5 cut(s) 88, 309, 543, 1062, 2269
BspLI GGNNCC 8 cut(s) 249, 685, 800, 1341, 1525, 1669, 1859, 2036
BspOI GCTAGC 1 cut(s) 1430
BspPI GGATC 1 cut(s) 2429
BspQI GCTCTTC 2 cut(s) 524, 1500
BspT104I TTCGAA 2 cut(s) 821, 1652
BspTI CTTAAG 1 cut(s) 503
BspTNI GGTCTC 1 cut(s) 858
BsrBI CCGCTC 1 cut(s) 1179
BsrDI GCAATG 4 cut(s) 629, 1124, 1323, 1610
BsrFI RCCGGY 2 cut(s) 314, 801
BsrI ACTGG 5 cut(s) 33, 141, 1041, 2076, 2184
BssAI RCCGGY 2 cut(s) 314, 801
BssECI CCNNGG 9 cut(s) 679, 1214, 1336, 1395, 1753, 1968, 2086, 2361, 2379
BssMI GATC 5 cut(s) 1090, 1633, 1660, 2214, 2434
BssSI CACGAG 1 cut(s) 1353
BssT1I CCWWGG 3 cut(s) 1214, 1395, 2379
Bst2BI CACGAG 1 cut(s) 1353
Bst2UI CCWGG 3 cut(s) 1775, 1969, 2232
Bst4CI ACNGT 4 cut(s) 42, 602, 1757, 2090
Bst6I CTCTTC 3 cut(s) 524, 612, 1500
BstAFI CTTAAG 1 cut(s) 503
BstBI TTCGAA 2 cut(s) 821, 1652
BstC8I GCNNGC 6 cut(s) 490, 494, 1392, 1428, 1575, 2534
BstDEI CTNAG 9 cut(s) 96, 296, 551, 636, 791, 1070, 1368, 2152, 2256
BstDSI CCRYGG 2 cut(s) 1753, 2086
BstEII GGTNACC 1 cut(s) 2226
BstF5I GGATG 3 cut(s) 534, 1224, 2212
BstH2I RGCGCY 1 cut(s) 2430
BstHHI GCGC 1 cut(s) 2429
BstKTI GATC 5 cut(s) 1093, 1636, 1663, 2217, 2437
BstMAI GTCTC 4 cut(s) 97, 149, 858, 1200
BstMBI GATC 5 cut(s) 1090, 1633, 1660, 2214, 2434
BstMWI GCNNNNNNNGC 4 cut(s) 277, 640, 1364, 1521
BstNI CCWGG 3 cut(s) 1775, 1969, 2232
BstNSI RCATGY 2 cut(s) 2242, 2536
BstPI GGTNACC 1 cut(s) 2226
BstSCI CCNGG 9 cut(s) 224, 383, 678, 1335, 1336, 1717, 1773, 1967, 2230
BstSFI CTRYAG 1 cut(s) 1590
BstSLI GKGCMC 2 cut(s) 1343, 2504
BstV1I GCAGC 6 cut(s) 613, 627, 1304, 1340, 1768, 2208
BstV2I GAAGAC 2 cut(s) 311, 1983
BstX2I RGATCY 2 cut(s) 1633, 2214
BstXI CCANNNNNNTGG 1 cut(s) 2071
BstYI RGATCY 2 cut(s) 1633, 2214
BsuRI GGCC 6 cut(s) 678, 813, 1341, 1524, 1696, 2480
BtgI CCRYGG 2 cut(s) 1753, 2086
BtgZI GCGATG 1 cut(s) 2364
BtsCI GGATG 3 cut(s) 534, 1224, 2212
BtsI GCAGTG 1 cut(s) 2058
BtsIMutI CAGTG 6 cut(s) 38, 756, 1539, 1763, 2058, 2069
Cac8I GCNNGC 6 cut(s) 490, 494, 1392, 1428, 1575, 2534
CaiI CAGNNNCTG 1 cut(s) 1589
CfoI GCGC 1 cut(s) 2429
Cfr10I RCCGGY 2 cut(s) 314, 801
Cfr13I GGNCC 9 cut(s) 242, 1339, 1340, 1523, 1694, 1750, 1858, 2456, 2479
Cfr9I CCCGGG 1 cut(s) 1336
CpoI CGGWCCG 1 cut(s) 242
Csp6I GTAC 6 cut(s) 827, 1012, 1260, 1382, 1586, 1934
CspAI ACCGGT 2 cut(s) 314, 801
CspI CGGWCCG 1 cut(s) 242
CviAII CATG 9 cut(s) 174, 674, 1554, 1730, 1885, 1916, 2028, 2239, 2533
CviQI GTAC 6 cut(s) 827, 1012, 1260, 1382, 1586, 1934
DdeI CTNAG 9 cut(s) 96, 296, 551, 636, 791, 1070, 1368, 2152, 2256
DpnI GATC 5 cut(s) 1092, 1635, 1662, 2216, 2436
DpnII GATC 5 cut(s) 1090, 1633, 1660, 2214, 2434
DriI GACNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 3 cut(s) 524, 612, 1500
Eam1105I GACNNNNNGTC 1 cut(s) 583
EarI CTCTTC 3 cut(s) 524, 612, 1500
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 1214, 1395, 2379
Eco24I GRGCYC 3 cut(s) 237, 686, 1343
Eco31I GGTCTC 1 cut(s) 858
Eco32I GATATC 2 cut(s) 2495, 2563
Eco47I GGWCC 4 cut(s) 242, 1750, 1858, 2456
Eco47III AGCGCT 1 cut(s) 2428
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 3 cut(s) 1701, 2031, 2504
Eco88I CYCGRG 4 cut(s) 979, 1336, 2220, 2360
Eco91I GGTNACC 1 cut(s) 2226
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 3 cut(s) 1340, 1523, 2479
EcoO65I GGTNACC 1 cut(s) 2226
EcoRI GAATTC 4 cut(s) 546, 703, 974, 1725
EcoRII CCWGG 3 cut(s) 1773, 1967, 2230
EcoRV GATATC 2 cut(s) 2495, 2563
EcoT14I CCWWGG 3 cut(s) 1214, 1395, 2379
EcoT22I ATGCAT 1 cut(s) 2538
EcoT38I GRGCYC 3 cut(s) 237, 686, 1343
ErhI CCWWGG 3 cut(s) 1214, 1395, 2379
FaeI CATG 9 cut(s) 177, 677, 1557, 1733, 1888, 1919, 2031, 2242, 2536
FalI AAGNNNNNCTT 2 cut(s) 1379, 1411
FaqI GGGAC 2 cut(s) 1871, 2434
FatI CATG 9 cut(s) 173, 673, 1553, 1729, 1884, 1915, 2027, 2238, 2532
Fnu4HI GCNGC 9 cut(s) 301, 627, 641, 1318, 1329, 1782, 1925, 2197, 2243
FokI GGATG 3 cut(s) 521, 1231, 2219
FriOI GRGCYC 3 cut(s) 237, 686, 1343
Fsp4HI GCNGC 9 cut(s) 301, 627, 641, 1318, 1329, 1782, 1925, 2197, 2243
FspBI CTAG 7 cut(s) 840, 947, 966, 1032, 1427, 1445, 2127
GlaI GCGC 1 cut(s) 2428
GluI GCNGC 9 cut(s) 301, 627, 641, 1318, 1329, 1782, 1925, 2197, 2243
GsaI CCCAGC 3 cut(s) 663, 1857, 2392
HaeII RGCGCY 1 cut(s) 2430
HaeIII GGCC 6 cut(s) 678, 813, 1341, 1524, 1696, 2480
HapII CCGG 9 cut(s) 225, 240, 245, 315, 384, 679, 802, 1337, 1718
HhaI GCGC 1 cut(s) 2429
Hin1II CATG 9 cut(s) 177, 677, 1557, 1733, 1888, 1919, 2031, 2242, 2536
Hin6I GCGC 1 cut(s) 2427
HinP1I GCGC 1 cut(s) 2427
HincII GTYRAC 1 cut(s) 2022
HindII GTYRAC 1 cut(s) 2022
HindIII AAGCTT 2 cut(s) 278, 1388
HpaI GTTAAC 1 cut(s) 2022
HpaII CCGG 9 cut(s) 225, 240, 245, 315, 384, 679, 802, 1337, 1718
HphI GGTGA 4 cut(s) 304, 1750, 2220, 2395
Hpy166II GTNNAC 3 cut(s) 1753, 2022, 2502
Hpy8I GTNNAC 3 cut(s) 1753, 2022, 2502
Hpy99I CGWCG 2 cut(s) 437, 589
HpyCH4III ACNGT 4 cut(s) 42, 602, 1757, 2090
HpyCH4IV ACGT 1 cut(s) 435
HpyF10VI GCNNNNNNNGC 4 cut(s) 277, 640, 1364, 1521
HpyF3I CTNAG 9 cut(s) 96, 296, 551, 636, 791, 1070, 1368, 2152, 2256
HpySE526I ACGT 1 cut(s) 435
Hsp92II CATG 9 cut(s) 177, 677, 1557, 1733, 1888, 1919, 2031, 2242, 2536
HspAI GCGC 1 cut(s) 2427
KspAI GTTAAC 1 cut(s) 2022
Kzo9I GATC 5 cut(s) 1090, 1633, 1660, 2214, 2434
LguI GCTCTTC 2 cut(s) 524, 1500
LmnI GCTCC 8 cut(s) 232, 336, 689, 769, 1349, 1372, 1834, 1956
Lsp1109I GCAGC 6 cut(s) 613, 627, 1304, 1340, 1768, 2208
LweI GCATC 4 cut(s) 543, 1301, 2362, 2545
MaeI CTAG 7 cut(s) 840, 947, 966, 1032, 1427, 1445, 2127
MaeII ACGT 1 cut(s) 435
MaeIII GTNAC 6 cut(s) 842, 1073, 2039, 2226, 2447, 2576
MalI GATC 5 cut(s) 1092, 1635, 1662, 2216, 2436
MbiI CCGCTC 1 cut(s) 1179
MboI GATC 5 cut(s) 1090, 1633, 1660, 2214, 2434
MfeI CAATTG 1 cut(s) 847
MflI RGATCY 2 cut(s) 1633, 2214
MhlI GDGCHC 8 cut(s) 237, 686, 1343, 1354, 1377, 1961, 2071, 2504
MmeI TCCRAC 6 cut(s) 210, 222, 393, 756, 2300, 2527
Mph1103I ATGCAT 1 cut(s) 2538
MslI CAYNNNNRTG 1 cut(s) 2115
MspA1I CMGCKG 4 cut(s) 614, 1317, 2199, 2507
MspCI CTTAAG 1 cut(s) 503
MspI CCGG 9 cut(s) 225, 240, 245, 315, 384, 679, 802, 1337, 1718
MspR9I CCNGG 9 cut(s) 226, 385, 680, 1337, 1338, 1719, 1775, 1969, 2232
MunI CAATTG 1 cut(s) 847
Mva1269I GAATGC 2 cut(s) 1047, 1992
MvaI CCWGG 3 cut(s) 1775, 1969, 2232
MwoI GCNNNNNNNGC 4 cut(s) 277, 640, 1364, 1521
NciI CCSGG 6 cut(s) 226, 385, 680, 1337, 1338, 1719
NdeII GATC 5 cut(s) 1090, 1633, 1660, 2214, 2434
NheI GCTAGC 1 cut(s) 1426
NlaIII CATG 9 cut(s) 177, 677, 1557, 1733, 1888, 1919, 2031, 2242, 2536
NlaIV GGNNCC 8 cut(s) 249, 685, 800, 1341, 1525, 1669, 1859, 2036
NmeAIII GCCGAG 1 cut(s) 1045
NmuCI GTSAC 3 cut(s) 2039, 2226, 2447
NsiI ATGCAT 1 cut(s) 2538
NspI RCATGY 2 cut(s) 2242, 2536
NspV TTCGAA 2 cut(s) 821, 1652
PaeI GCATGC 1 cut(s) 2536
PaeR7I CTCGAG 1 cut(s) 2220
PciSI GCTCTTC 2 cut(s) 524, 1500
PcsI WCGNNNNNNNCGW 2 cut(s) 420, 429
PctI GAATGC 2 cut(s) 1047, 1992
PflMI CCANNNNNTGG 1 cut(s) 1476
PinAI ACCGGT 2 cut(s) 314, 801
PkrI GCNGC 9 cut(s) 302, 628, 642, 1319, 1330, 1783, 1926, 2198, 2244
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 3 cut(s) 1773, 1967, 2230
PspEI GGTNACC 1 cut(s) 2226
PspFI CCCAGC 3 cut(s) 659, 1853, 2388
PspGI CCWGG 3 cut(s) 1773, 1967, 2230
PspN4I GGNNCC 8 cut(s) 249, 685, 800, 1341, 1525, 1669, 1859, 2036
PspOMI GGGCCC 1 cut(s) 1339
PspPI GGNCC 9 cut(s) 242, 1339, 1340, 1523, 1694, 1750, 1858, 2456, 2479
PstNI CAGNNNCTG 1 cut(s) 1589
PsuI RGATCY 2 cut(s) 1633, 2214
PvuII CAGCTG 3 cut(s) 614, 1317, 2507
RsaI GTAC 6 cut(s) 828, 1013, 1261, 1383, 1587, 1935
RsaNI GTAC 6 cut(s) 827, 1012, 1260, 1382, 1586, 1934
RseI CAYNNNNRTG 1 cut(s) 2115
Rsr2I CGGWCCG 1 cut(s) 242
RsrII CGGWCCG 1 cut(s) 242
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 2 cut(s) 524, 1500
SatI GCNGC 9 cut(s) 301, 627, 641, 1318, 1329, 1782, 1925, 2197, 2243
Sau3AI GATC 5 cut(s) 1090, 1633, 1660, 2214, 2434
Sau96I GGNCC 9 cut(s) 242, 1339, 1340, 1523, 1694, 1750, 1858, 2456, 2479
ScrFI CCNGG 9 cut(s) 226, 385, 680, 1337, 1338, 1719, 1775, 1969, 2232
SduI GDGCHC 8 cut(s) 237, 686, 1343, 1354, 1377, 1961, 2071, 2504
SfaNI GCATC 4 cut(s) 543, 1301, 2362, 2545
SfcI CTRYAG 1 cut(s) 1590
Sfr274I CTCGAG 1 cut(s) 2220
SfuI TTCGAA 2 cut(s) 821, 1652
SinI GGWCC 4 cut(s) 242, 1750, 1858, 2456
SlaI CTCGAG 1 cut(s) 2220
SmaI CCCGGG 1 cut(s) 1338
SmiMI CAYNNNNRTG 1 cut(s) 2115
SmlI CTYRAG 3 cut(s) 350, 503, 2220
SmoI CTYRAG 3 cut(s) 350, 503, 2220
SphI GCATGC 1 cut(s) 2536
SsiI CCGC 6 cut(s) 301, 1179, 1736, 1925, 2199, 2243
SspMI CTAG 7 cut(s) 840, 947, 966, 1032, 1427, 1445, 2127
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 9 cut(s) 224, 383, 678, 1335, 1336, 1717, 1773, 1967, 2230
StyI CCWWGG 3 cut(s) 1214, 1395, 2379
TaaI ACNGT 4 cut(s) 42, 602, 1757, 2090
TaiI ACGT 1 cut(s) 438
TaqII GACCGA 1 cut(s) 404
TatI WGTACW 1 cut(s) 1259
TauI GCSGC 3 cut(s) 303, 1927, 2245
TscAI CASTG 6 cut(s) 45, 756, 1546, 1770, 2065, 2076
TseFI GTSAC 3 cut(s) 2039, 2226, 2447
TseI GCWGC 6 cut(s) 626, 640, 1317, 1328, 1781, 2196
Tsp45I GTSAC 3 cut(s) 2039, 2226, 2447
TspDTI ATGAA 6 cut(s) 117, 162, 662, 716, 1718, 2099
TspGWI ACGGA 1 cut(s) 2027
TspMI CCCGGG 1 cut(s) 1336
TspRI CASTG 6 cut(s) 45, 756, 1546, 1770, 2065, 2076
Van91I CCANNNNNTGG 1 cut(s) 1476
Vha464I CTTAAG 1 cut(s) 503
VneI GTGCAC 1 cut(s) 2500
VpaK11BI GGWCC 4 cut(s) 242, 1750, 1858, 2456
XceI RCATGY 2 cut(s) 2242, 2536
XhoI CTCGAG 1 cut(s) 2220
XmaI CCCGGG 1 cut(s) 1336
XspI CTAG 7 cut(s) 840, 947, 966, 1032, 1427, 1445, 2127
Zsp2I ATGCAT 1 cut(s) 2538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.